JP6424161B2 - Fermented milk-containing beverage - Google Patents

Fermented milk-containing beverage Download PDF

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JP6424161B2
JP6424161B2 JP2015522727A JP2015522727A JP6424161B2 JP 6424161 B2 JP6424161 B2 JP 6424161B2 JP 2015522727 A JP2015522727 A JP 2015522727A JP 2015522727 A JP2015522727 A JP 2015522727A JP 6424161 B2 JP6424161 B2 JP 6424161B2
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fermented milk
milk
beverage
pectin
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JPWO2014199876A1 (en
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桃子 宗
桃子 宗
康伸 大塚
康伸 大塚
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Asahi Soft Drinks Co Ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00Milk preparations; Milk powder or milk powder preparations
    • A23C9/12Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/13Fermented milk preparations; Treatment using microorganisms or enzymes using additives
    • A23C9/137Thickening substances
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38Other non-alcoholic beverages
    • A23L2/382Other non-alcoholic beverages fermented
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • A23L2/66Proteins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

Description

本発明は、保存時において長期間乳蛋白質の凝集、沈殿及び離水が抑制され、さらに風味として糊感がなく、喉越しが良好な発酵乳含有飲料に関する。   The present invention relates to a fermented milk-containing beverage that suppresses aggregation, precipitation, and water separation of milk proteins for a long period of time during storage, and that does not have a sticky feeling as a flavor and has a good feeling over the throat.

乳性飲料を製造するに当たり、発酵乳を多く含有する乳性飲料の場合には、飲料中の乳蛋白質の粒子径が大きくなり、保存時に該乳蛋白質を主とする沈殿量が増え、また離水が発生する。これは、発酵乳中の乳蛋白質粒子が、発酵過程で生成した酸による変性を受け、凝集や沈殿を生じやすいためである。保存安定性を向上させるための方策として、安定剤の添加や均質化処理を行うことが一般的に知られているが、十分な効果を得ることができていない。
例えば、非特許文献1には、安定剤の添加量は乳蛋白質粒子径、乳成分含有量と賞味期間を考慮し決定することが記載されている。さらに、粒子径が大きい発酵乳を使用する場合は、ペクチンなどの粘性が高い安定剤を使用すること、酸性化乳の場合は粒子径が比較的小さいので粘性の少ない大豆多糖類でも保存安定性を保つことができること、乳固形分が多い製品には両者を併用する場合もあることが記載されている。また、酸性化乳、発酵乳どちらを使用するにしても製品の保存安定性を保つためには、均質化処理をする必要があることが記載されている。すなわち、乳蛋白質粒子を微細化し安定化させる以外に、安定剤や乳化剤を使用する場合の分散効率を上げるためにホモゲナイザーを用い、10〜20MPaの圧力下で均質化処理することが記載されている。さらに、均質化後の80%粒子径が3μmを越える製品は沈殿が多く商品価値が保てないことが記載されている。
In the production of a dairy beverage, in the case of a dairy beverage that contains a large amount of fermented milk, the particle size of the milk protein in the beverage increases, the amount of precipitation mainly consisting of the milk protein increases during storage, and water separation occurs. Will occur. This is because the milk protein particles in the fermented milk are subject to denaturation by the acid generated during the fermentation process, and tend to cause aggregation and precipitation. As a measure for improving storage stability, it is generally known to add a stabilizer or homogenize, but a sufficient effect cannot be obtained.
For example, Non-Patent Document 1 describes that the amount of stabilizer added is determined in consideration of milk protein particle size, milk component content and shelf life. In addition, when using fermented milk with a large particle size, use a stabilizer with high viscosity such as pectin. In the case of acidified milk, the particle size is relatively small so that even soybean polysaccharides with low viscosity can be stored stably. It is described that both can be used together in products with a high milk solid content. Moreover, it is described that it is necessary to perform a homogenization treatment in order to maintain the storage stability of the product regardless of whether acidified milk or fermented milk is used. That is, in addition to making the milk protein particles finer and stabilized, it is described that a homogenizer is used under a pressure of 10 to 20 MPa in order to increase the dispersion efficiency when a stabilizer or an emulsifier is used. . Further, it is described that a product having an 80% particle diameter of more than 3 μm after homogenization has a large amount of precipitation and cannot maintain a commercial value.

また、特許文献1は、乳に酸味料を添加してpHを3.0〜4.2に調整する乳含有酸性乳飲料においては、大豆食物繊維及びペクチンを使用し、10〜50MPaの圧力下で均質化することで、長期間の保存において凝集・沈殿が実質的に生じることのない、風味に優れ、保存安定性良好な乳含有酸性乳飲料の製造方法を開示している。しかし、特許文献1の酸性乳飲料は発酵乳を含まないものであり、発酵過程で変性を受けた乳蛋白質粒子は含有されておらず、保存安定化させることが極度に困難な飲料ではない。
特許文献2は、安定剤、糖類を溶解し、これに乳成分を混合溶解後、酸性物質を添加してpHを3.5〜4.5に調整し、500〜1500kg/cm2で加圧して均質化処理することにより得られる、保存中に沈殿が少ない酸性乳飲料及びその製造方法を開示している。
特許文献3は、乳性蛋白質含有酸性飲料の製造方法として、発酵乳に糖類等を添加し均質化した後に加熱殺菌して容器充填後、冷却して振とう処理を行う方法を開示している。
Patent Document 1 discloses that in a milk-containing acidic milk beverage in which a sour agent is added to milk and the pH is adjusted to 3.0 to 4.2, soy dietary fiber and pectin are used under a pressure of 10 to 50 MPa. The method for producing a milk-containing acidic milk beverage having excellent flavor and good storage stability, in which aggregation and precipitation do not substantially occur during long-term storage, is disclosed. However, the acidic milk drink of Patent Document 1 does not contain fermented milk, does not contain milk protein particles that have been modified during the fermentation process, and is not a drink that is extremely difficult to preserve and stabilize.
In Patent Document 2, a stabilizer and a saccharide are dissolved, and milk components are mixed and dissolved therein, then an acidic substance is added to adjust the pH to 3.5 to 4.5, and the pressure is increased to 500 to 1500 kg / cm 2. An acidic milk beverage that is obtained by homogenization and has less precipitation during storage and a method for producing the same are disclosed.
Patent Document 3 discloses a method for producing a milky protein-containing acidic beverage by adding sugars and the like to fermented milk, homogenizing it, heat-sterilizing it, filling the container, and then cooling and shaking. .

また、特許文献4は、低酸度で発酵させたヨーグルト原液に果汁を加え、250〜550気圧、55〜75℃で均質化を行った後、溶存酸素を除去し、密封容器に充填後加熱処理することで、品質が安定して長期間分離せず、褐変及び酸化が極めて少ない風味の高い嗜好性のよい果汁入りヨーグルト飲料が製造できることを開示している。
特許文献5は、発酵乳の原料に添加する乳酸菌として乳酸桿菌及び乳酸球菌を用いる液状発酵乳の製造方法であって、50〜100MPaの均質化圧力で液状発酵乳を得る均質化工程を含む、液状発酵乳の製造方法を開示している。
特許文献6は、殺菌液状発酵乳及び殺菌乳酸菌飲料を製造する際に、ハイメトキシル(HM)ペクチン及び発酵乳を加え、pHを調整して混合液とした後、30℃以上の加熱下で均質化処理を行う製造法を開示している。
Moreover, patent document 4 adds fruit juice to the yogurt stock solution fermented by low acidity, homogenizes at 250-550 atmospheres and 55-75 degreeC, removes dissolved oxygen, and heat-processes after filling a sealed container. By doing so, it is disclosed that a yogurt drink containing fruit juice with good taste and high flavor that has a stable quality and does not separate for a long period of time and has very little browning and oxidation can be produced.
Patent Document 5 is a method for producing liquid fermented milk that uses lactobacilli and lactic acid cocci as lactic acid bacteria to be added to the raw material of fermented milk, and includes a homogenization step of obtaining liquid fermented milk at a homogenizing pressure of 50 to 100 MPa. A method for producing liquid fermented milk is disclosed.
Patent Document 6 discloses that when producing sterilized liquid fermented milk and sterilized lactic acid bacteria beverages, high methoxyl (HM) pectin and fermented milk are added, and the pH is adjusted to obtain a mixed solution, which is then homogeneously heated at 30 ° C. or higher. The manufacturing method which performs a chemical conversion treatment is disclosed.

特開2001−190254号公報JP 2001-190254 A 特開平5−43号公報Japanese Patent Laid-Open No. 5-43 特開平6−319449号公報JP-A-6-319449 特開平6−22688号公報Japanese Patent Laid-Open No. 6-22688 特許第4963747号公報Japanese Patent No. 4963747 特開平3−285641号公報JP-A-3-2855641

「最新・ソフトドリンクス」、株式会社光琳、平成15年9月30日、p.365−378“Latest Soft Drinks”, Kozo Co., Ltd., September 30, 2003, p. 365-378

上記のように、乳成分に酸味料を加えて酸性化する飲料における製造方法や、ヨーグルト原液あるいは発酵乳に対して加熱条件下及び/又は高圧条件下で均質化処理するヨーグルトドリンク様飲料の製造方法が知られている。しかしながら、発酵乳を含み、長期間乳蛋白質の凝集及び沈殿が抑制され、外観上の商品価値が保たれ、さらに風味として糊感がなく、喉越しが良好な発酵乳含有飲料及びその製造方法は知られていない。   As described above, a production method in a beverage that is acidified by adding a sour agent to a milk component, or a yogurt drink-like beverage that is homogenized under heating conditions and / or high-pressure conditions on a yogurt stock solution or fermented milk The method is known. However, fermented milk-containing beverages that contain fermented milk, that prevent aggregation and precipitation of milk proteins for a long period of time, maintain the commercial value of the appearance, have no sticky feeling as a flavor, and have a good feeling over the throat unknown.

本発明の課題は、発酵過程で変性を受けた乳蛋白質粒子を含み、長期保存安定化が極度に困難な発酵乳含有飲料において、長期間乳蛋白質の凝集及び沈殿、並びに離水が抑制され(以後、単に、長期保存安定性と称することもある)、さらに風味として糊感がなく、喉越しが良好な発酵乳含有飲料を提供することにある。即ち、良好な風味と長期保存安定性を両立した発酵乳含有飲料を提供することを目的とする。
また、本発明の別の観点の課題は、長期保存安定性と良好な風味を両立できる、発酵乳含有飲料の製造方法を提供することにある。更に、発酵乳を多く含有する発酵乳含有飲料の長期保存安定性に有効な、該発酵乳含有飲料の離水及び沈殿抑制方法を提供することにある。
An object of the present invention is to contain milk protein particles that have been modified during the fermentation process, and in a fermented milk-containing beverage that is extremely difficult to stabilize for a long period of time, aggregation and precipitation of milk protein for a long period of time and water separation are suppressed (hereinafter referred to as “water storage”). Furthermore, the present invention is to provide a fermented milk-containing beverage that does not have a sticky feeling as a flavor and has a good feeling over the throat. That is, an object of the present invention is to provide a fermented milk-containing beverage that has both good flavor and long-term storage stability.
Moreover, the subject of another viewpoint of this invention is providing the manufacturing method of fermented milk containing drink which can make long-term storage stability and favorable flavor compatible. Furthermore, it is providing the water separation and precipitation suppression method of this fermented milk containing drink effective for the long-term storage stability of the fermented milk containing drink containing much fermented milk.

本発明者らは、上記課題を解決するために鋭意検討を行った結果、大豆多糖類及びHMペクチンを、飲料全量に対して特定の含有量で、かつ両者を特定の比率で配合使用することにより、無脂乳固形分の多い発酵乳を多く含有する、長期保存安定性と良好な風味を両立させた発酵乳含有飲料及びその製造方法を開発し、本発明を完成するに至った。更に、長期保存安定性に資する、発酵乳含有飲料の離水及び沈殿抑制方法の発明も完成させた。   As a result of intensive studies to solve the above-mentioned problems, the inventors of the present invention use soy polysaccharide and HM pectin in a specific content with respect to the total amount of beverage and use both in a specific ratio. Has developed a fermented milk-containing beverage that contains a large amount of non-fat milk solid content fermented milk and has both long-term storage stability and good flavor, and a method for producing the same, and has completed the present invention. Furthermore, the invention of the water separation and precipitation suppression method of fermented milk containing drinks which contribute to long-term storage stability was also completed.

即ち、本発明によれば、無脂乳固形分を含む発酵乳と、大豆多糖類と、HMペクチンと、を含有し、前記無脂乳固形分の含有量が、飲料全量に対して0.5〜6.0質量%であり、前記大豆多糖類及び前記HMペクチンの合計含有量が、飲料全量に対して0.3〜0.8質量%であり、前記大豆多糖類と前記HMペクチンとの含有比率が、質量比で3:1〜1:4である発酵乳液であって、該発酵乳液を温度50〜90℃及び圧力30〜70MPaの条件で均質化処理することにより、飲料中の乳蛋白質粒子のメジアン径が0.7μm以下である、発酵乳含有飲料が提供される。
That is, according to this invention, fermented milk containing non-fat milk solid content, soybean polysaccharide, and HM pectin are contained, and the content of the non-fat milk solid content is 0. 5 to 6.0% by mass, and the total content of the soybean polysaccharide and the HM pectin is 0.3 to 0.8% by mass with respect to the total amount of beverage, and the soybean polysaccharide and the HM pectin Is a fermented milk emulsion having a mass ratio of 3: 1 to 1: 4 , and the fermented milk liquid is homogenized under conditions of a temperature of 50 to 90 ° C. and a pressure of 30 to 70 MPa. A fermented milk-containing beverage in which the median diameter of milk protein particles is 0.7 μm or less is provided.

また、本発明によれば、発酵乳に、大豆多糖類及びHMペクチンを、前記大豆多糖類及び前記HMペクチンの合計含有量が飲料全量に対して0.3〜0.8質量%、かつ前記大豆多糖類と前記HMペクチンとの含有比率が質量比で3:1〜1:4となるように添加して発酵乳液を得る工程と、前記発酵乳液中の無脂乳固形分の含有量を、飲料全量に対して0.5〜6.0質量%に調整する工程と、前記発酵乳液を、温度50〜90℃及び圧力30〜70MPaの条件で均質化処理する工程と、を有する、発酵乳含有飲料の製造方法が提供される。   According to the present invention, the fermented milk contains soybean polysaccharide and HM pectin, and the total content of the soybean polysaccharide and HM pectin is 0.3 to 0.8% by mass with respect to the total amount of beverage, and The step of adding fermented milk so that the content ratio of soybean polysaccharide and the HM pectin is 3: 1 to 1: 4 by mass ratio, and the content of non-fat milk solids in the fermented milk And a step of adjusting to 0.5 to 6.0% by mass with respect to the total amount of beverage, and a step of homogenizing the fermented milk under conditions of a temperature of 50 to 90 ° C. and a pressure of 30 to 70 MPa. A method for producing a milk-containing beverage is provided.

更に、本発明によれば、無脂乳固形分を0.5〜6.0質量%含有する発酵乳に、大豆多糖類及びHMペクチンを、前記大豆多糖類及び前記HMペクチンの合計含有量が飲料全量に対して0.3〜0.8質量%、かつ前記大豆多糖類と前記HMペクチンとの含有比率が質量比で3:1〜1:4となるように添加し、温度50〜90℃及び圧力30〜70MPaの条件で均質化処理を実施する、発酵乳含有飲料の離水及び沈殿抑制方法が提供される。   Furthermore, according to the present invention, fermented milk containing 0.5 to 6.0% by mass of non-fat milk solids, soybean polysaccharide and HM pectin, and the total content of soybean polysaccharide and HM pectin are It is added so that the content ratio of the soy polysaccharide and the HM pectin is 3: 1 to 1: 4 by mass ratio with respect to the total amount of the beverage, and the temperature is 50 to 90. Provided is a method for inhibiting water separation and precipitation of a fermented milk-containing beverage, wherein the homogenization is performed under the conditions of ° C and a pressure of 30 to 70 MPa.

本発明の発酵乳含有飲料は、大豆多糖類及びHMペクチンを、飲料全量に対して特定の含有量で、かつ両者を特定の比率で配合使用しているので、保存時において長期間乳蛋白質の凝集及び沈殿、並びに離水が抑制され、さらに風味として糊感がなく、喉越しが良好な発酵乳含有飲料を提供することができる。また、発酵乳を多く含有する飲料でありながら、常温で4ヶ月またはそれ以上の長期間に渡って安定性を維持できるので、常温流通可能な発酵乳含有飲料として提供することができる。
また、本発明の製造方法は、無脂乳固形分の多い発酵乳を多く含有する、長期保存安定性と良好な風味を両立させた発酵乳含有飲料を製造でき、また、本発明の発酵乳含有飲料の離水及び沈殿抑制方法は、当該発酵乳を多く含有する発酵乳含有飲料の安定的な長期保存に有効である。
The fermented milk-containing beverage of the present invention uses soy polysaccharides and HM pectin in a specific content with respect to the total amount of the beverage and uses both in a specific ratio. It is possible to provide a fermented milk-containing beverage in which aggregation and precipitation, and water separation are suppressed, and there is no paste feeling as a flavor, and the throat is good. Moreover, since it is a drink which contains many fermented milk, stability can be maintained over a long period of four months or more at normal temperature, Therefore It can provide as a fermented milk containing drink which can be distributed at normal temperature.
In addition, the production method of the present invention can produce a fermented milk-containing beverage having both long-term storage stability and good flavor, which contains a large amount of non-fat milk solid fermented milk, and the fermented milk of the present invention. The water separation and precipitation suppression method for the contained beverage is effective for stable long-term storage of the fermented milk-containing beverage containing a large amount of the fermented milk.

実施例4,5及び比較例6,7の「とろみ」の評価結果を示すグラフ図である。It is a graph which shows the evaluation result of "Thoromi" of Examples 4 and 5 and Comparative Examples 6 and 7. 実施例4,5及び比較例6,7の「とろみの好ましさ」の評価結果を示すグラフ図である。It is a graph which shows the evaluation result of Examples 4 and 5 and Comparative Examples 6 and 7 "preference for thickness". 実施例4,5及び比較例6,7の「喉越しの良さ」の評価結果を示すグラフ図である。It is a graph which shows the evaluation result of "goodness over the throat" of Examples 4 and 5 and Comparative Examples 6 and 7. 実施例4,5及び比較例6,7の「全体の風味」の評価結果を示すグラフ図である。It is a graph which shows the evaluation result of "the whole flavor" of Examples 4 and 5 and Comparative Examples 6 and 7.

以下、本発明を更に詳細に説明する。
本発明の発酵乳を得るための原料乳としては、動物又は植物由来の乳等、いずれの乳をも用いることができる。例えば、牛乳、山羊乳、羊乳、及び馬乳等の獣乳、並びに豆乳等の植物乳が挙げられる。乳の入手し易さの点で牛乳が好ましい。なお、これらの乳は、単独で、又は2種類以上が配合されて使用されても良い。これらの乳を原料乳として使用する場合、その形態は特に限定されず、全脂乳、脱脂乳あるいは乳清、さらには乳蛋白質濃縮物等を用いることができ、また、粉乳、濃縮乳から還元した乳も使用できる。
Hereinafter, the present invention will be described in more detail.
As the raw material milk for obtaining the fermented milk of the present invention, any milk such as animal or plant-derived milk can be used. Examples thereof include animal milk such as cow's milk, goat milk, sheep milk, and horse milk, and vegetable milk such as soy milk. Milk is preferable from the viewpoint of easy availability of milk. In addition, these milk may be used individually or in mixture of 2 or more types. When these milks are used as raw material milk, the form is not particularly limited, and whole milk, skim milk or whey, and milk protein concentrate can be used, and reduced from powdered milk and concentrated milk. Milk can also be used.

本発明で使用する発酵乳は、上記原料乳の微生物による発酵工程を経て製造される発酵酸性乳である。微生物による発酵方法は、通常の発酵乳製造に使用される発酵方法で行えばよく、静置発酵、攪拌発酵、振とう発酵、通気発酵などが挙げられる。発酵は、通常30〜40℃の温度で、pHが酸性になるまで行なえばよい。好ましくはpH3.3〜3.8である。
また、本発明で使用する発酵乳は、予め均質化処理が施されていることが望ましく、さらに、当該発酵乳中の乳蛋白質粒子のメジアン径が1〜10μmであることが好ましく、1〜5μmであることがより好ましい。
この場合の均質化処理は、ホモゲナイザーを用いて実施することが好ましい。他にはダイノーミル等、食品加工に一般に用いられる均質化装置を使用できる。使用するホモゲナイザーの段数(一段式、多段式)については特に限定されない。
均質化温度は、5〜25℃が好ましく、5〜15℃がさらに好ましい。また、均質化圧力については特に限定されないが、15〜50MPaが好ましい。
The fermented milk used in the present invention is fermented acidic milk produced through a fermentation process by microorganisms of the raw material milk. The fermentation method using microorganisms may be a fermentation method used for ordinary fermented milk production, and examples thereof include stationary fermentation, stirring fermentation, shake fermentation, and aeration fermentation. Fermentation is usually performed at a temperature of 30 to 40 ° C. until the pH becomes acidic. Preferably it is pH 3.3-3.8.
Moreover, it is desirable that the fermented milk used in the present invention is subjected to a homogenization treatment in advance, and the median diameter of milk protein particles in the fermented milk is preferably 1 to 10 μm, and preferably 1 to 5 μm. It is more preferable that
The homogenization treatment in this case is preferably performed using a homogenizer. In addition, a homogenizer generally used for food processing such as dyno mill can be used. There are no particular restrictions on the number of homogenizers used (single-stage, multi-stage).
The homogenization temperature is preferably 5 to 25 ° C, more preferably 5 to 15 ° C. Moreover, it does not specifically limit about a homogenization pressure, However, 15-50 Mpa is preferable.

本発明で使用する大豆多糖類は、大豆製品の製造工程において副成するオカラから抽出精製された多糖類であり、含有されるガラクツロン酸のカルボキシル基に由来して酸性下マイナスに帯電しているものであれば良い。なお、市販のものを用いることができ、例えば、「SM−1200(三栄源エフ・エフ・アイ株式会社製)」を使用できる。
大豆多糖類は、乳蛋白質を含めた乳固形分量が比較的少ない、清涼飲料水に分類されるような酸性の乳性飲料に対する安定化剤として使用される。しかし、本発明が対象とする、発酵過程において変性を受けた乳蛋白質量の多い発酵乳を多く含む飲料(発酵乳含有飲料)に対しては、単独使用ではその安定化性能が不十分であり、長期間に渡って離水、及び乳蛋白質粒子の凝集・沈殿を抑制することができない。
The soybean polysaccharide used in the present invention is a polysaccharide extracted and purified from okara that is a by-product in the production process of soybean products, and is negatively charged under acidity derived from the carboxyl group of galacturonic acid contained therein. Anything is fine. In addition, a commercially available thing can be used, for example, "SM-1200 (made by San-Ei Gen FFI Co., Ltd.)" can be used.
Soy polysaccharides are used as stabilizers for acidic dairy drinks, which are classified as soft drinks, having a relatively low milk solid content including milk proteins. However, for a beverage (fermented milk-containing beverage) containing a large amount of fermented milk with a large amount of milk protein that has been modified during the fermentation process, which is the subject of the present invention, its stabilization performance is insufficient when used alone. In addition, water separation over a long period of time and aggregation / precipitation of milk protein particles cannot be suppressed.

本発明で使用するHMペクチンとは、主に柑橘類から抽出される多糖類である。市販のHMペクチンを用いることができ、エステル化度が65〜75%のものが好適である。例えば、「YM−115−LJ(三晶株式会社製)」等を使用できる。
なお、HMペクチンは酸性の乳性飲料の安定化剤として使用される。しかし、本発明が対象とする、発酵過程において変性を受けた乳蛋白質量の多い発酵乳を多く含む飲料に対しては、大豆多糖類同様、単独使用ではその安定化性能が不十分であり、長期間に渡って離水、及び乳蛋白質粒子の凝集・沈殿を抑制することができない。
The HM pectin used in the present invention is a polysaccharide mainly extracted from citrus fruits. Commercially available HM pectin can be used, and those having a degree of esterification of 65 to 75% are suitable. For example, “YM-115-LJ (manufactured by Sanki Co., Ltd.)” or the like can be used.
HM pectin is used as a stabilizer for acidic milk beverages. However, for beverages containing a large amount of fermented milk with a large amount of milk protein that has been modified during the fermentation process, which is the subject of the present invention, as in the case of soybean polysaccharides, its sole use performance is insufficient, Water separation and aggregation / precipitation of milk protein particles cannot be suppressed over a long period of time.

また、発酵過程において変性を受けた乳蛋白質量の多い発酵乳を多く含む飲料に対して、単に大豆多糖類とHMペクチンを配合使用するだけでは、離水防止、及び乳蛋白質粒子の凝集・沈殿抑制の点で、満足のいく効果を得ることができない。
発酵過程による酸性度の変化等、発酵に伴う変性の影響を受けた乳蛋白質等の無脂乳固形分粒子は、当該無脂乳固形分自体が多く含有されていることとも相まって、発酵乳中での長期間の分散安定化が困難となる。発酵過程において、乳蛋白質粒子等の無脂乳固形分粒子に、何らかの凝集点が形成されることが予想されるが、明確なことは解明されていない。
このように安定性に問題のある発酵乳含有飲料の長期保存安定性を達成するためには、糖を添加して糖度の高い、即ち、ブリックス(Brix)値の高い飲料とすることが従来から実施されている手段であった。これは、糖の分散力によって長期保存安定性を付与するという技術である。従って、糖度を抑え(低ブリックス値の)まろやかかつ爽やかな風味を有する発酵乳含有飲料とするということは、その発想自体ほとんど考えられてこなかった。
In addition, by simply using soy polysaccharide and HM pectin in combination with fermented milk with a large amount of milk protein that has been modified in the fermentation process, it is possible to prevent water separation and to suppress aggregation and precipitation of milk protein particles. In this respect, a satisfactory effect cannot be obtained.
Non-fat milk solids particles such as milk proteins affected by fermentation-related modification such as changes in acidity during the fermentation process, coupled with the fact that the non-fat milk solids themselves are contained in a large amount, It becomes difficult to stabilize the dispersion for a long time. In the fermentation process, some aggregation point is expected to be formed on the non-fat milk solid particles such as milk protein particles, but the clearness has not been elucidated.
Thus, in order to achieve long-term storage stability of a fermented milk-containing beverage having a problem with stability, it has been conventionally necessary to add sugar to obtain a beverage having a high sugar content, that is, a high Brix value. It was the means being implemented. This is a technique of imparting long-term storage stability by the dispersibility of sugar. Therefore, it has hardly been thought of itself as a fermented milk-containing beverage with a low sugar content (low Brix value) and a mellow and refreshing flavor.

以上のように、本発明の発酵乳含有飲料は、発酵に伴う変性の影響を受けた乳蛋白質等の無脂乳固形分を多く含有し、具体的には、無脂乳固形分を0.5〜6.0質量%含有するものである。無脂乳固形分の含有量の下限は、0.9質量%がより好ましく、上限としては5.0質量%がより好ましく、4.0質量%がさらに好ましい。
無脂乳固形分が0.5質量%未満では、発酵乳含有飲料に期待される「風味の厚み」が感じられず満足のいく味とならない。一方、無脂乳固形分が6.0質量%を超えると、乳蛋白質粒子の凝集・沈殿抑制が極度に困難となる。
As described above, the fermented milk-containing beverage of the present invention contains a large amount of non-fat milk solids such as milk proteins affected by the denaturation associated with fermentation. 5 to 6.0% by mass is contained. The lower limit of the content of the non-fat milk solid is more preferably 0.9% by mass, the upper limit is more preferably 5.0% by mass, and further preferably 4.0% by mass.
If the non-fat milk solid content is less than 0.5% by mass, the “flavor thickness” expected for a fermented milk-containing beverage is not felt, and a satisfactory taste is not achieved. On the other hand, when the non-fat milk solid content exceeds 6.0% by mass, it is extremely difficult to suppress aggregation and precipitation of milk protein particles.

このように、発酵に伴う変性の影響を受けた無脂乳固形分を多く含む(すなわち、乳蛋白質粒子を多く含む)発酵乳を多く含有する飲料は、長期保存安定性と良好な風味を両立させることが極めて困難であるところ、大豆多糖類及びHMペクチンの特異的な配合使用により、本発明はその両立を達成したものである。
すなわち、本発明においては、大豆多糖類及びHMペクチンの合計含有量が飲料全量に対して0.3〜0.8質量%、かつ大豆多糖類とHMペクチンとの含有比率が質量比で3:1〜1:4となるように添加された発酵乳含有飲料とすることにより、顕著に良好な離水防止、及び乳蛋白質粒子の凝集・沈殿抑制を達成し得たものである。
大豆多糖類とHMペクチンの含有比率が質量比で3:1〜1:4であるという条件において、大豆多糖類とHMペクチンを上記範囲で配合使用することが必要である。両者の合計含有量が0.3質量%未満では、発酵乳含有飲料中で乳蛋白質を長期間安定的に分散させることができず、凝集及び沈殿が発生する恐れがある。一方、両者の合計含有量が0.8質量%を超えると、得られる飲料の風味不良が生じたり、粘度が高くなり糊感が生じて清涼感が失われたりする恐れがある。
なお、より好ましくは、上記要件を満足する条件において、HMペクチンの含有量を飲料全量に対して0.1〜0.4質量%とする。長期保存安定性と良好な清涼感を高度に両立できるからである。特に、0.4質量%以下であれば、糊感の発生を良好に抑制することができる。
In this way, beverages containing a large amount of non-fat milk solids that are affected by fermentation-related denaturation (ie, containing a lot of milk protein particles) have both long-term storage stability and good flavor. Although it is extremely difficult to achieve this, the present invention achieves the compatibility by using a specific blend of soybean polysaccharide and HM pectin.
That is, in the present invention, the total content of soybean polysaccharide and HM pectin is 0.3 to 0.8% by mass relative to the total amount of beverage, and the content ratio of soybean polysaccharide and HM pectin is 3: By making the fermented milk-containing beverage added so as to be 1 to 1: 4, it is possible to achieve remarkably good prevention of water separation and suppression of aggregation and precipitation of milk protein particles.
On the condition that the content ratio of soybean polysaccharide and HM pectin is 3: 1 to 1: 4 in terms of mass ratio, it is necessary to blend and use soybean polysaccharide and HM pectin within the above range. If the total content of both is less than 0.3% by mass, the milk protein cannot be stably dispersed in the fermented milk-containing beverage for a long period of time, and aggregation and precipitation may occur. On the other hand, if the total content of both exceeds 0.8% by mass, the resulting beverage may have poor flavor, or the viscosity may increase and a feeling of paste may occur, resulting in a loss of refreshing feeling.
In addition, More preferably, content of HM pectin shall be 0.1-0.4 mass% with respect to drink whole quantity on the conditions which satisfy | fill the said requirements. This is because long-term storage stability and a good refreshing feeling can be achieved at a high level. In particular, when the content is 0.4% by mass or less, the occurrence of a sticky feeling can be satisfactorily suppressed.

本発明の発酵乳含有飲料は、喉越しの良さ及び清涼感の点で、ブリックス値が20未満であることが好ましく、10〜19がより好ましい。ブリックス値が20未満であることは、飲料中における乳蛋白質粒子の安定的な分散に寄与すると考えられる糖の含有量が少ないということであり、上記した通り、本発明が目指した発酵乳含有飲料は、離水防止、及び乳蛋白質粒子の凝集・沈殿抑制が非常に難しい飲料である。
なお、本発明におけるブリックス値は、20℃における糖用屈折計の示度であり、例えばデジタル屈折計Rx-5000(アタゴ社製)を使用して20℃で測定した固形分量とすることができる。
The fermented milk-containing beverage of the present invention preferably has a Brix value of less than 20 and more preferably 10 to 19 from the viewpoint of good feeling over the throat and a refreshing feeling. A Brix value of less than 20 means that the sugar content, which is considered to contribute to the stable dispersion of milk protein particles in the beverage, is low, and as described above, the fermented milk-containing beverage aimed at by the present invention. Is a beverage that is very difficult to prevent water separation and to suppress aggregation and precipitation of milk protein particles.
The Brix value in the present invention is an indication of a refractometer for sugar at 20 ° C., and can be a solid content measured at 20 ° C. using a digital refractometer Rx-5000 (manufactured by Atago Co., Ltd.), for example. .

ブリックス値の調整は、例えば、糖類や甘味料を含有させる方法により行うことができる。糖類としては、例えば、砂糖、果糖、ぶどう糖、乳糖、麦芽糖等の単糖や二糖、エリスリトール、マルチトール等の糖アルコールが挙げられる。甘味料としては、例えば、スクラロース、アスパルテーム、アセスルファムカリウム、ステビア等の高甘味度甘味料が挙げられる。このとき、最終製品の発酵乳含有飲料におけるブリックス値が上記のように20未満となるようにすることが望ましい。   The Brix value can be adjusted by, for example, a method of containing a saccharide or a sweetener. Examples of the saccharide include monosaccharides such as sugar, fructose, glucose, lactose, and maltose, and sugar alcohols such as disaccharides, erythritol, and maltitol. Examples of the sweetener include high-intensity sweeteners such as sucralose, aspartame, acesulfame potassium, stevia and the like. At this time, it is desirable that the Brix value in the fermented milk-containing beverage of the final product is less than 20 as described above.

本発明の発酵乳含有飲料は酸性であることが好ましく、そのpHとして2.5〜4.5が好ましく、3.0〜4.0がより好ましい。pHがこの範囲であれば喉越しが良く清涼感のある発酵乳含有飲料とすることができる。
本発明の発酵乳含有飲料のpHを調整するために、酸味料や、果汁等を使用することができる。酸味料としては、例えば、乳酸、クエン酸、リンゴ酸、酒石酸、酢酸、フィチン酸、グルコン酸、コハク酸、フマール酸等の有機酸やリン酸等の無機酸や、それらの塩が挙げられる。果汁としては、例えば、オレンジ、レモン、グレープフルーツ、グレープ、モモ、リンゴ等の果汁が挙げられる。また、酸味料や果汁等を使用せず、発酵乳の発酵度を調節してpHを上記好ましい範囲に調整してもよい。
The fermented milk-containing beverage of the present invention is preferably acidic, and its pH is preferably 2.5 to 4.5, more preferably 3.0 to 4.0. If pH is this range, it can be set as the fermented milk containing drink with a good throat feeling and a refreshing feeling.
In order to adjust the pH of the fermented milk-containing beverage of the present invention, acidulant, fruit juice, and the like can be used. Examples of the acidulant include organic acids such as lactic acid, citric acid, malic acid, tartaric acid, acetic acid, phytic acid, gluconic acid, succinic acid, and fumaric acid, inorganic acids such as phosphoric acid, and salts thereof. Examples of the fruit juice include fruit juices such as orange, lemon, grapefruit, grape, peach, and apple. Moreover, you may adjust pH of the fermented milk to the said preferable range by adjusting the degree of fermentation of fermented milk, without using a sour agent, fruit juice, etc.

本発明の発酵乳含有飲料は、その他の成分として、所望により、全脂乳、脱脂乳あるいは乳清、さらには乳蛋白質濃縮物等、また、粉乳、濃縮乳から還元した乳を用いてもよく、その他、クリーム、練乳等の乳製品を配合してもよい。   The fermented milk-containing beverage of the present invention may use, as other components, milk, skim milk or whey, milk protein concentrate, milk powder reduced from milk powder or concentrated milk, as desired. In addition, dairy products such as cream and condensed milk may be blended.

本発明の発酵乳含有飲料中の乳蛋白質粒子の粒子径(直径)は、メジアン径として0.7μm以下である。ここで、メジアン径とは、粒度分布を有する粒子群の粒度を表す指標として一般的に用いられる値であり、分布の中央値に対応する粒子径である。
メジアン径は、粒度分布測定装置、例えば、(株)堀場製作所製の型式LA−920等を用いて測定することができる。
The particle diameter (diameter) of the milk protein particles in the fermented milk-containing beverage of the present invention is 0.7 μm or less as the median diameter. Here, the median diameter is a value generally used as an index representing the particle size of a particle group having a particle size distribution, and is a particle size corresponding to the median value of the distribution.
The median diameter can be measured using a particle size distribution measuring apparatus, for example, model LA-920 manufactured by Horiba, Ltd.

また、本発明の発酵乳含有飲料においては、飲料としての一般的な粘度であれば特に限定されるものではないが、粘度が高くなると糊感が生じ、とろみとして好ましくなく、清涼感が失われるので、室温下で、10mPa・s以下の粘度であることが好ましい。
なお、発酵乳含有飲料の粘度は、例えば、芝浦セムテック株式会社製のビスメトロン粘度計を用いて測定することができる。
In addition, the fermented milk-containing beverage of the present invention is not particularly limited as long as it has a general viscosity as a beverage, but when the viscosity becomes high, a feeling of paste is generated, which is not preferable as a thickening, and a refreshing feeling is lost. Therefore, the viscosity is preferably 10 mPa · s or less at room temperature.
The viscosity of the fermented milk-containing beverage can be measured, for example, using a bismetholone viscometer manufactured by Shibaura Semtec Co., Ltd.

次に、本発明の発酵乳含有飲料の製造方法について説明する。
原乳を、乳酸菌等を使用して発酵させた後、ホモゲナイザーで均質化処理して発酵乳を得る。この段階での均質化は、例えば、温度5〜25℃、圧力15MPaの条件で行う。当該条件以外であっても、発酵乳中の乳蛋白質粒子のメジアン径が1〜10μmとなるように均質化できれば、他の均質化条件を採用してもよい。
つづいて、上記発酵乳に大豆多糖類溶液及びHMペクチン溶液、並びに所望により他の添加成分を加え、必要に応じて精製水等の水により濃度を調整する。なお、この中間段階のものを便宜上発酵乳液と称することとする。
その後、更に、ホモゲナイザーによる発酵乳液の均質化処理を実施する。この段階での均質化条件としては次の通りである。まず、温度は下限として50℃以上が好ましく、60℃以上がより好ましい。一方、上限としては、90℃以下が好ましく、80℃以下がより好ましい。圧力としては、下限として30MPa以上が好ましく、40MPa以上がより好ましく、50MPa以上がさらに好ましい。一方、上限としては、70MPa以下が好ましい。
Next, the manufacturing method of the fermented milk containing drink of this invention is demonstrated.
Raw milk is fermented using lactic acid bacteria and the like, and then homogenized with a homogenizer to obtain fermented milk. Homogenization at this stage is performed, for example, under conditions of a temperature of 5 to 25 ° C. and a pressure of 15 MPa. Even if it is other than the said conditions, as long as it can homogenize so that the median diameter of the milk protein particle | grains in fermented milk may become 1-10 micrometers, you may employ | adopt other homogenization conditions.
Subsequently, the soybean polysaccharide solution and the HM pectin solution, and other additive components as desired are added to the fermented milk, and the concentration is adjusted with water such as purified water as necessary. This intermediate stage is referred to as fermented milk for convenience.
Then, the homogenization process of the fermented milk liquid by a homogenizer is further implemented. The homogenization conditions at this stage are as follows. First, the lower limit of the temperature is preferably 50 ° C. or higher, and more preferably 60 ° C. or higher. On the other hand, as an upper limit, 90 degrees C or less is preferable and 80 degrees C or less is more preferable. The lower limit of the pressure is preferably 30 MPa or more, more preferably 40 MPa or more, and further preferably 50 MPa or more. On the other hand, the upper limit is preferably 70 MPa or less.

発酵乳液に上記均質化処理を実施した後、容器充填して40℃以下に冷却した後に振とう処理を施す。振とう処理は、機械的振とうが好ましく、往復又は、回転運動等を伴う方式で連続処理できる装置を使用することにより行うのが好ましい。具体的には、350mL容器を例とした場合、ストローク10〜30cmで1〜2往復/秒、3〜6秒程度の往復運動、又は容器の長手方向で1〜2回転/秒、3〜6秒程度の回転運動の振とう処理が好ましい。容器容量が異なる場合は、上記と同程度の振とう処理となるように調整すればよい。   After performing the said homogenization process to fermented milk liquid, the container is filled, and after cooling to 40 degrees C or less, a shaking process is performed. The shaking treatment is preferably mechanical shaking, and is preferably performed by using an apparatus capable of continuous treatment in a manner involving reciprocation or rotational motion. Specifically, when a 350 mL container is taken as an example, a stroke of 10 to 30 cm is 1 to 2 reciprocations / second, a reciprocation of about 3 to 6 seconds, or 1 to 2 rotations / second in the longitudinal direction of the container, 3 to 6 A shaking process with a rotational motion of about a second is preferred. If the container capacities are different, adjustment may be made so that the shaking process is similar to the above.

以上の工程により、本発明の発酵乳含有飲料を製造することができる。なお、飲料であるので、殺菌処理工程を施すことが好ましい。殺菌処理工程は、発酵乳液の均質化処理の前後に行うか、容器充填前後に行う。殺菌処理の方法は、特に制限されず、通常のプレート式殺菌、チューブラー式殺菌、レトルト殺菌、バッチ殺菌、オートクレーブ殺菌などの方法を採用することができる。   By the above process, the fermented milk-containing beverage of the present invention can be produced. In addition, since it is a drink, it is preferable to perform a sterilization process. The sterilization treatment step is performed before or after the homogenization treatment of the fermented milk liquid or before and after filling the container. The method of sterilization treatment is not particularly limited, and methods such as ordinary plate sterilization, tubular sterilization, retort sterilization, batch sterilization, and autoclave sterilization can be employed.

本発明の発酵乳含有飲料に使用する飲料容器は、特に限定されるものではないが、ガラス、プラスチック(ポリエチレンテレフタレート(PET)、ポリエチレン(PE)、ポリプロピレン(PP)等)、紙、アルミ、スチール製の密封容器が挙げられる。また、容量についても特に限定はされないが、通常50mL〜10L程度である。   The beverage container used for the fermented milk-containing beverage of the present invention is not particularly limited, but glass, plastic (polyethylene terephthalate (PET), polyethylene (PE), polypropylene (PP), etc.), paper, aluminum, steel For example, a hermetically sealed container is used. Moreover, although it does not specifically limit about a capacity | capacitance, Usually, it is about 50mL-10L.

以下本発明を実施例により説明するが、本発明はこれらに限定されない。
<発酵乳含有飲料の評価方法>
1.乳蛋白質粒子径
発酵乳含有飲料の製造直後、及び長期保存後について、発酵乳含有飲料中の乳蛋白質粒子の粒径(直径)及び粒度分布を、粒度分布測定装置(型式LA−920、(株)堀場製作所製)を用いて測定した。なお、粒径としてはメジアン径を採用した。
なお、長期保存の条件は、容器充填後37℃で4ヶ月間の静置保存とした。以後の評価においても同様である。
Hereinafter, the present invention will be described with reference to examples, but the present invention is not limited thereto.
<Method for evaluating fermented milk-containing beverage>
1. Milk protein particle size Immediately after the production of the fermented milk-containing beverage and after long-term storage, the particle size (diameter) and particle size distribution of the milk protein particles in the fermented milk-containing beverage are measured using a particle size distribution measuring device (model LA-920, strain ) Manufactured by HORIBA, Ltd.). The median diameter was adopted as the particle diameter.
The long-term storage conditions were set at 4 ° C. for 4 months after container filling. The same applies to the subsequent evaluation.

2.離水性
長期保存後の離水性の評価として、下記式により算出した値を離水度と規定し、離水度によって評価した。数値の大きい方が、離水が少なく良好であることを示す。
長期保存後の発酵乳含有飲料(350mL容量のPET充填品)の底面から高さ1/2の位置から、内容液をサンプリングし、得られた内溶液を10倍に希釈した後、吸光度A(650nm)を測定した。また、保存飲料全体を振とうして十分に分散させて均一にした後、その分散後の内容液を10倍に希釈し、吸光度B(650nm)を測定した。そして、次式によって、離水度C(%)を算出した。
C(%)=A/B×100
2. As an evaluation of water separation after long-term storage, the value calculated by the following formula was defined as the water separation degree, and the water separation degree was evaluated. Larger values indicate better water removal.
After the long-term storage, the content liquid was sampled from a position at a height of 1/2 from the bottom of the fermented milk-containing beverage (350 mL-capacity PET-filled product). 650 nm). Further, the whole stored beverage was shaken and sufficiently dispersed to make it uniform, and then the content liquid after the dispersion was diluted 10 times, and the absorbance B (650 nm) was measured. And water separation degree C (%) was computed by following Formula.
C (%) = A / B × 100

3.沈殿物の生成
沈殿物生成の状態を、長期保存後の発酵乳含有飲料(350mL容量のPET充填品)について、底部沈殿物の厚さ(沈降厚:mm)を計測することによって評価した。
3. Formation of Precipitate The state of precipitate formation was evaluated by measuring the thickness of the bottom sediment (sediment thickness: mm) for the fermented milk-containing beverage (350 mL capacity filled with PET) after long-term storage.

4.粘度
発酵乳含有飲料の製造直後の25℃下での粘度を、ビスメトロン粘度計(型式 VDA型、芝浦セムテック株式会社製)により測定した。ローターとしてはNo.1ローターを使用した。
4). The viscosity at 25 ° C. immediately after the production of the fermented milk-containing beverage was measured with a bismetholone viscometer (model VDA type, manufactured by Shibaura Semtec Co., Ltd.). As a rotor, No. One rotor was used.

5.風味等の評価(官能評価)
5℃に冷却した製造直後の発酵乳含有飲料について以下のように官能評価を行った。判定員として習熟したパネル13名により、「とろみ」、「とろみの好ましさ」、「喉越しの良さ」、及び「全体の風味」の各項目について評価した。
「とろみ」は下記の採点基準に従って、0〜8点まで、その他の項目については下記基準に従って、−4〜+4点までの、9段階で評価し、その平均点数を算出した。
(とろみ)
0点:ない
2点:わずかにある
4点:ややある
6点:ある
8点:かなりある
(とろみの好ましさ)
−4点:好ましくない
−2点:やや好ましくない
0点:どちらでもない
+2点:やや好ましい
+4点:好ましい
(喉越しの良さ、及び全体の風味)
−4点:悪い
−2点:やや悪い
0点:どちらでもない
+2点:やや良い
+4点:良い
5). Evaluation of flavor, etc. (sensory evaluation)
Sensory evaluation was performed as follows about the fermented milk containing drink immediately after manufacture cooled to 5 degreeC. Thirteen panel members who were trained as judgers evaluated each item of “Thoromi”, “Preferred Thoromi”, “Good throat”, and “Overall flavor”.
“Toromi” was evaluated in 9 stages from 0 to 8 points according to the following scoring standards, and other items from −4 to +4 points according to the following standards, and the average score was calculated.
(Toromi)
0 points: No 2 points: Slightly 4 points: Slightly 6 points: Yes 8 points: Very good (preference for thoroughness)
-4 points: unfavorable -2 points: somewhat unfavorable 0 points: neither of them +2 points: slightly preferable +4 points: preferable (goodness over the throat and overall flavor)
-4 points: Bad -2 points: Slightly bad 0 points: Neither of them +2 points: Slightly good +4 points: Good

実施例1
9.0質量%還元脱脂粉乳を、乳酸菌を用いて37℃で22時間発酵させた後、10℃下、ホモゲナイザーで、圧力15MPaで均質化処理を行った。得られた発酵乳中の乳蛋白質粒子のメジアン径は、4.2957μmであった。
次に、得られた発酵乳122.2gに対し、グラニュ糖101.6gを添加して混合溶解した。次いで、2質量%大豆多糖類水溶液50g及び2質量%HMペクチン水溶液200gを添加し、さらに、香料を添加した。最後にイオン交換水を用いて全量を1000gとした(発酵乳液)。大豆多糖類及びHMペクチンの飲料全体に対する含有量は表1に示した通りである。なお、大豆多糖類としては「SM−1200(三栄源エフ・エフ・アイ株式会社製)」、HMペクチンとしては「YM−115−LJ(三晶株式会社製)」を使用した。
得られた発酵乳液を70℃に加熱した状態で、ホモゲナイザーを用いて圧力50MPaで均質化処理を行った。その後、加熱殺菌して350mL容量のPETボトルにホットパック充填した。つづいて、流水中で40℃以下になるまで静置冷却した後、長手方向で2回転/秒、3秒程度の回転運動の振とう処理を実施し、本発明の発酵乳含有飲料とした。
得られた実施例1の発酵乳含有飲料について、上記1.乳蛋白質粒子径、2.離水性、及び3.沈殿物の生成について評価した。結果を表1に示す。
Example 1
After 9.0 mass% reduced skim milk was fermented using lactic acid bacteria at 37 ° C. for 22 hours, homogenization was performed at 10 ° C. with a homogenizer at a pressure of 15 MPa. The median diameter of milk protein particles in the obtained fermented milk was 4.2957 μm.
Next, 101.6 g of granulated sugar was added to 122.2 g of the obtained fermented milk and mixed and dissolved. Next, 50 g of a 2 mass% soybean polysaccharide aqueous solution and 200 g of a 2 mass% HM pectin aqueous solution were added, and a flavor was further added. Finally, the total amount was made 1000 g (fermented milky lotion) using ion-exchanged water. The content of soybean polysaccharide and HM pectin with respect to the whole beverage is as shown in Table 1. In addition, "SM-1200 (manufactured by San-Ei Gen FFI Co., Ltd.)" was used as the soybean polysaccharide, and "YM-115-LJ (manufactured by Sanki Co., Ltd.)" was used as the HM pectin.
The obtained fermented milk was heated to 70 ° C. and homogenized at a pressure of 50 MPa using a homogenizer. After that, it was heat sterilized and filled in a 350 mL capacity PET bottle with a hot pack. Subsequently, after standing and cooling to 40 ° C. or lower in running water, a shaking treatment with a rotational motion of about 2 rotations / second and about 3 seconds in the longitudinal direction was performed to obtain the fermented milk-containing beverage of the present invention.
Regarding the obtained fermented milk-containing beverage of Example 1, the above 1. Milk protein particle size, 2. 2. water separation, and The formation of precipitate was evaluated. The results are shown in Table 1.

実施例2〜5、比較例1〜5
大豆多糖類及びHMペクチンについて、表1に示した含有量とした以外は実施例1と同様にして発酵乳含有飲料を製造し、実施例1と同様に評価した。結果を表1に示す。
Examples 2-5, Comparative Examples 1-5
About the soybean polysaccharide and HM pectin, except having set it as content shown in Table 1, the fermented milk containing drink was manufactured like Example 1, and it evaluated similarly to Example 1. FIG. The results are shown in Table 1.

Figure 0006424161
Figure 0006424161

表1から明確なように、実施例1〜5の発酵乳含有飲料は、乳蛋白質粒子径、離水度、及び沈殿厚のすべてにおいて良好な結果であり、長期保存安定性に優れていた。
それに反し、比較例1は乳蛋白質粒子径が大きいため、沈殿厚も大きく沈殿量が多かった。また、比較例2、3、4は離水度が最も小さいため飲料の上部は離水しており、さらに沈殿厚も大きく沈殿量が多く、保存安定性において満足いくものではなかった。さらに、比較例5は離水度が小さいため飲料の上部は離水していた。これは、各比較例が、大豆多糖類とHMペクチンの全飲料に対する含有量、及び配合割合が本発明の範囲を外れているためである。
また、各実施例及び各比較例の結果から、乳蛋白質粒子径のメジアン径が、粒径及びその経時安定性の点で良好であっても、発酵乳含有飲料の長期安定性に大きな差が存在することが判り、大豆多糖類とHMペクチンの全飲料に対する含有量、及び配合割合を、本発明のように特定することが重要である。
As is clear from Table 1, the fermented milk-containing beverages of Examples 1 to 5 had good results in all of the milk protein particle diameter, the water separation degree, and the precipitation thickness, and were excellent in long-term storage stability.
On the other hand, Comparative Example 1 had a large milk protein particle size, so the precipitation thickness was large and the amount of precipitation was large. Further, in Comparative Examples 2, 3, and 4, since the water separation degree was the smallest, the upper part of the beverage was water-separated, and the precipitation thickness was large and the amount of precipitation was large, so that the storage stability was not satisfactory. Furthermore, since the comparative example 5 had small water separation degree, the upper part of the drink was water-separating. This is because each comparative example has a content and blending ratio of soybean polysaccharide and HM pectin with respect to all beverages outside the scope of the present invention.
In addition, from the results of each example and each comparative example, even if the median diameter of the milk protein particle diameter is good in terms of the particle diameter and its stability over time, there is a large difference in the long-term stability of the fermented milk-containing beverage. It turns out that it exists and it is important to specify content and the mixture ratio with respect to all the drinks of soybean polysaccharide and HM pectin like this invention.

実施例4及び5で製造した発酵乳含有飲料について、4.粘度、及び5.風味等の評価について評価した。結果を表2、及び図1〜4に示す。   4. Regarding fermented milk-containing beverages produced in Examples 4 and 5. 4. viscosity, and The evaluation of flavor and the like was evaluated. The results are shown in Table 2 and FIGS.

比較例6、7
大豆多糖類及びHMペクチンについて、表2に示した含有量とした以外は実施例1と同様にして発酵乳含有飲料を製造し、実施例4及び5と同様に、4.粘度、及び5.風味等の評価について評価した。結果を表2、及び図1〜4に示す。
Comparative Examples 6 and 7
For soybean polysaccharides and HM pectin, a fermented milk-containing beverage was produced in the same manner as in Example 1 except that the content shown in Table 2 was used. 4. viscosity, and The evaluation of flavor and the like was evaluated. The results are shown in Table 2 and FIGS.

Figure 0006424161
Figure 0006424161

大豆多糖類及びHMペクチンの合計含有量が増加するほど、特にHMペクチンの含有量が増加するほど粘度が上がり、「とろみ」評価においてとろみが強く感じられた(表2、図1)。また、「とろみの好ましさ」については、実施例4及び5はともに好ましかったが、比較例6及び7はどちらでもないから好ましくない、の範囲であった(図2)。さらに、「喉越しの良さ」及び「全体の風味」についても、実施例4及び5はともに良かったが、比較例6及び7はどちらでもないから悪い、の範囲であった(図3、4)。
従って、実施例4及び5は、風味として糊感がなく、喉越しが良好な発酵乳含有飲料である。
As the total content of soybean polysaccharide and HM pectin increased, in particular, as the content of HM pectin increased, the viscosity increased, and thickening was felt strongly in the “thoroughness” evaluation (Table 2, FIG. 1). Moreover, about the "preference of thickening", although Example 4 and 5 were both preferable, since Comparative Examples 6 and 7 were neither, it was the range which is not preferable (FIG. 2). Furthermore, regarding “goodness over the throat” and “overall flavor”, Examples 4 and 5 were both good, but Comparative Examples 6 and 7 were neither in the range of being bad (FIGS. 3 and 4). ).
Therefore, Examples 4 and 5 are fermented milk-containing beverages that do not have a sticky feeling as a flavor and have a good feeling over the throat.

実施例6〜12、比較例8、9
均質化処理条件の温度及び圧力を表3に示した条件とした以外は、実施例4と同様にして発酵乳含有飲料を製造し、実施例4と同様に、1.乳蛋白質粒子径、2.離水性、及び3.沈殿物の生成について評価した。結果を表3に示す。
Examples 6 to 12, Comparative Examples 8 and 9
A fermented milk-containing beverage was produced in the same manner as in Example 4 except that the temperature and pressure of the homogenization treatment conditions were as shown in Table 3. Milk protein particle size, 2. 2. water separation, and The formation of precipitate was evaluated. The results are shown in Table 3.

参考例1、2
発酵乳を使用しない酸性化乳飲料を次のようにして製造し、参考例として同様に評価した。
20質量%の還元脱脂粉乳49.5gに対し、50質量%グラニュ糖水溶液196gを添加して混合溶解した。その後、2質量%大豆多糖類水溶液125gを添加した後、10質量%クエン酸水溶液20gを添加した。さらに、2質量%ペクチン水溶液125gを添加した。その後、香料を添加した。最後にイオン交換水を用いて全量を1000gとした。
得られた調合液を表3に示す条件で均質化処理を行った(温度、圧力以外は実施例1と同じ)。その後、加熱殺菌して350mL容量のPETボトルにホットパック充填した。つづいて、流水中で40℃以下になるまで静置冷却して参考例の酸性化乳飲料とした。なお、振とう処理は実施しなかった。
得られた参考例の酸性化乳飲料について、上記1.乳蛋白質粒子径、2.離水性、及び3.沈殿物の生成について実施例1と同様に評価した。結果を表3に示す。
Reference examples 1 and 2
An acidified milk beverage that does not use fermented milk was produced as follows and evaluated in the same manner as a reference example.
To 49.5 g of 20% by mass of reduced skim milk powder, 196 g of a 50% by mass aqueous granulated sugar solution was added and mixed and dissolved. Thereafter, 125 g of a 2 mass% soybean polysaccharide aqueous solution was added, and then 20 g of a 10 mass% citric acid aqueous solution was added. Further, 125 g of a 2 mass% pectin aqueous solution was added. Thereafter, a fragrance was added. Finally, the total amount was made 1000 g using ion-exchanged water.
The obtained preparation liquid was homogenized under the conditions shown in Table 3 (same as Example 1 except for temperature and pressure). After that, it was heat sterilized and filled in a 350 mL capacity PET bottle with a hot pack. Subsequently, it was allowed to stand still in flowing water until it became 40 ° C. or lower to obtain an acidified milk drink of Reference Example. In addition, the shaking process was not implemented.
About the acidified milk drink of the obtained reference example, said 1 .. Milk protein particle size, 2. 2. water separation, and The formation of the precipitate was evaluated in the same manner as in Example 1. The results are shown in Table 3.

実施例13
9.0質量%還元脱脂粉乳を、乳酸菌を用いて30℃で24時間発酵させた後、10℃下、ホモゲナイザーで、圧力15MPaで均質化処理を行った。得られた発酵乳中の乳蛋白質粒子のメジアン径は、4.7280μmであった。
次に、得られた発酵乳400.0gに対し、グラニュ糖131.3gを添加して混合溶解した。次いで、2質量%大豆多糖類水溶液125g及び2質量%HMペクチン水溶液125gを添加し、さらに、香料を添加した。最後にイオン交換水を用いて全量を1000gとした(発酵乳液)。
得られた発酵乳液について、実施例1と同様に均質化処理及び振とう処理を実施して発酵乳含有飲料を製造し、1.乳蛋白質粒子径、及び3.沈殿物の生成について実施例1と同様に評価した。2.離水性については、吸光度A、B測定の際に、内容液を40倍に希釈して測定した。結果を表3に示す。
Example 13
After 9.0 mass% reduced skim milk was fermented with lactic acid bacteria at 30 ° C. for 24 hours, homogenization was performed at 10 ° C. with a homogenizer at a pressure of 15 MPa. The median diameter of milk protein particles in the obtained fermented milk was 4.7280 μm.
Next, 131.3 g of granulated sugar was added to 400.0 g of the obtained fermented milk and mixed and dissolved. Subsequently, 125 g of 2 mass% soybean polysaccharide aqueous solution and 125 g of 2 mass% HM pectin aqueous solution were added, and a flavor was further added. Finally, the total amount was made 1000 g (fermented milky lotion) using ion-exchanged water.
About the obtained fermented milk liquid, the homogenization process and the shaking process are implemented like Example 1, and a fermented milk containing drink is manufactured, 1. 2. milk protein particle size, and The formation of the precipitate was evaluated in the same manner as in Example 1. 2. The water separation was measured by diluting the content liquid 40 times when measuring the absorbances A and B. The results are shown in Table 3.

比較例10
均質化処理条件の温度及び圧力を表3に示した条件とした以外は、実施例13と同様にして発酵乳含有飲料を製造し、実施例13と同様に、1.乳蛋白質粒子径、2.離水性、及び3.沈殿物の生成について評価した。結果を表3に示す。
Comparative Example 10
A fermented milk-containing beverage was produced in the same manner as in Example 13 except that the temperature and pressure of the homogenization treatment conditions were as shown in Table 3, and as in Example 13, 1. Milk protein particle size, 2. 2. water separation, and The formation of precipitate was evaluated. The results are shown in Table 3.

Figure 0006424161
Figure 0006424161

表3から明確なように、同一ブリックス値かつ同一無脂乳固形分量においては、温度50〜90℃、かつ圧力30〜70MPaの条件下で均質化処理を行うと、離水度、沈殿厚、粒子径のすべてにおいて良好な結果が得られた。特に、実施例13は保存後の粒子径の増大もみられず、良好な結果であった。
なお、参考例1及び2は、発酵乳を含有しない酸性化乳飲料であるため、均質化処理条件にかかわらず、また振とう処理を実施しなくても、離水度、沈殿厚、粒子径のすべてにおいて良好な結果が得られた(表3)。
As is clear from Table 3, at the same Brix value and the same non-fat milk solid content, when the homogenization treatment is performed under the conditions of a temperature of 50 to 90 ° C. and a pressure of 30 to 70 MPa, the water separation degree, the precipitation thickness, and the particles Good results were obtained for all of the diameters. In particular, Example 13 was a good result with no increase in the particle size after storage.
In addition, since Reference Examples 1 and 2 are acidified milk drinks that do not contain fermented milk, the degree of water separation, sedimentation thickness, and particle size can be obtained regardless of the homogenization treatment conditions and without performing the shaking treatment. Good results were obtained in all cases (Table 3).

Claims (8)

無脂乳固形分を含む発酵乳と、
大豆多糖類と、
HMペクチンと、を含有し、
前記無脂乳固形分の含有量が、飲料全量に対して0.5〜6.0質量%であり、
前記大豆多糖類及び前記HMペクチンの合計含有量が、飲料全量に対して0.3〜0.8質量%であり、
前記大豆多糖類と前記HMペクチンとの含有比率が、質量比で3:1〜1:4である発酵乳液であって、該発酵乳液を温度50〜90℃及び圧力30〜70MPaの条件で均質化処理することにより、飲料中の乳蛋白質粒子のメジアン径が0.7μm以下である
発酵乳含有飲料。
Fermented milk containing non-fat milk solids;
Soy polysaccharides,
HM pectin,
Content of the said non-fat milk solid content is 0.5-6.0 mass% with respect to the whole drink,
The total content of the soybean polysaccharide and the HM pectin is 0.3 to 0.8 mass% with respect to the total amount of beverage,
A fermented milk emulsion in which the content ratio of the soybean polysaccharide and the HM pectin is 3: 1 to 1: 4 in a mass ratio , and the fermented milk emulsion is homogeneous under conditions of a temperature of 50 to 90 ° C. and a pressure of 30 to 70 MPa. By performing the chemical treatment, the median diameter of the milk protein particles in the beverage is 0.7 μm or less ,
Fermented milk-containing beverage.
前記発酵乳含有飲料のブリックス値が20未満である、
請求項1に記載の発酵乳含有飲料。
The fermented milk-containing beverage has a Brix value of less than 20,
The fermented milk-containing beverage according to claim 1.
発酵乳に、大豆多糖類及びHMペクチンを、前記大豆多糖類及び前記HMペクチンの合計含有量が飲料全量に対して0.3〜0.8質量%、かつ前記大豆多糖類と前記HMペクチンとの含有比率が質量比で3:1〜1:4となるように添加する工程と、
無脂乳固形分の含有量を、飲料全量に対して0.5〜6.0質量%に調整して発酵乳液を得る工程と、
前記発酵乳液を、温度50〜90℃及び圧力30〜70MPaの条件で均質化処理する工程と、を有する、
発酵乳含有飲料の製造方法。
Fermented milk, soybean polysaccharide and HM pectin, the total content of the soybean polysaccharide and HM pectin is 0.3 to 0.8% by mass with respect to the total amount of beverage, and the soybean polysaccharide and HM pectin A step of adding so that the content ratio is 3: 1 to 1: 4 by mass ratio,
Adjusting the content of non-fat milk solids to 0.5 to 6.0% by mass with respect to the total amount of beverage to obtain a fermented milk,
A step of homogenizing the fermented milk under conditions of a temperature of 50 to 90 ° C and a pressure of 30 to 70 MPa.
A method for producing a fermented milk-containing beverage.
前記均質化処理後に、40℃以下の温度で、往復又は回転運動による機械的振とう処理する工程を有する、
請求項に記載の発酵乳含有飲料の製造方法。
After the homogenization treatment, a step of performing mechanical shaking treatment by reciprocating or rotating motion at a temperature of 40 ° C. or less,
The manufacturing method of the fermented milk containing drink of Claim 3 .
前記均質化処理前に、前記発酵乳含有飲料のブリックス値を20未満に調整する工程を有する、
請求項3又は4に記載の発酵乳含有飲料の製造方法。
Before the homogenization treatment, the step of adjusting the Brix value of the fermented milk-containing beverage to less than 20,
The manufacturing method of the fermented milk containing drink of Claim 3 or 4 .
発酵乳に、大豆多糖類及びHMペクチンを、前記大豆多糖類及び前記HMペクチンの合計含有量が飲料全量に対して0.3〜0.8質量%、かつ前記大豆多糖類と前記HMペクチンとの含有比率が質量比で3:1〜1:4となるように添加し、
温度50〜90℃及び圧力30〜70MPaの条件で均質化処理を実施する、
無脂乳固形分の含有量が飲料全量に対して0.5〜6.0質量%である、発酵乳含有飲料の離水及び沈殿抑制方法。
Fermented milk, soybean polysaccharide and HM pectin, the total content of the soybean polysaccharide and HM pectin is 0.3 to 0.8% by mass with respect to the total amount of beverage, and the soybean polysaccharide and HM pectin Is added so that the content ratio is 3: 1 to 1: 4 by mass ratio,
A homogenization treatment is performed under conditions of a temperature of 50 to 90 ° C. and a pressure of 30 to 70 MPa.
A method for inhibiting water separation and precipitation of fermented milk-containing beverages, wherein the content of non-fat milk solids is 0.5 to 6.0% by mass relative to the total amount of beverage.
前記均質化処理後に、40℃以下の温度で、往復又は回転運動による機械的振とう処理を実施する、
請求項に記載の発酵乳含有飲料の離水及び沈殿抑制方法。
After the homogenization treatment, a mechanical shaking treatment by reciprocating or rotating motion is performed at a temperature of 40 ° C. or less.
The water separation and precipitation suppression method of the fermented milk-containing beverage according to claim 6 .
前記発酵乳含有飲料のブリックス値を20未満に調整する、
請求項6又は7に記載の発酵乳含有飲料の離水及び沈殿抑制方法。
Adjusting the Brix value of the fermented milk-containing beverage to less than 20;
The water separation and precipitation suppression method of the fermented milk-containing beverage according to claim 6 or 7 .
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