JP6421977B2 - 検出特性を向上させた変異プロテアーゼバイオセンサー - Google Patents
検出特性を向上させた変異プロテアーゼバイオセンサーInfo
- Publication number
- JP6421977B2 JP6421977B2 JP2014541370A JP2014541370A JP6421977B2 JP 6421977 B2 JP6421977 B2 JP 6421977B2 JP 2014541370 A JP2014541370 A JP 2014541370A JP 2014541370 A JP2014541370 A JP 2014541370A JP 6421977 B2 JP6421977 B2 JP 6421977B2
- Authority
- JP
- Japan
- Prior art keywords
- luciferase
- circular permutation
- granzyme
- cell
- thermostable luciferase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161558796P | 2011-11-11 | 2011-11-11 | |
| US61/558,796 | 2011-11-11 | ||
| PCT/US2012/064675 WO2013071237A1 (en) | 2011-11-11 | 2012-11-12 | Mutant protease biosensors with enhanced detection characteristics |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015501636A JP2015501636A (ja) | 2015-01-19 |
| JP2015501636A5 JP2015501636A5 (https=) | 2016-01-14 |
| JP6421977B2 true JP6421977B2 (ja) | 2018-11-14 |
Family
ID=47222333
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014541370A Active JP6421977B2 (ja) | 2011-11-11 | 2012-11-12 | 検出特性を向上させた変異プロテアーゼバイオセンサー |
Country Status (3)
| Country | Link |
|---|---|
| EP (2) | EP2776573A1 (https=) |
| JP (1) | JP6421977B2 (https=) |
| WO (1) | WO2013071237A1 (https=) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2308978B8 (en) | 2003-10-10 | 2015-04-08 | Promega Corporation | Luciferase biosensor |
| EP2004813B1 (en) | 2006-04-03 | 2015-06-03 | Promega Corporation | Permuted and nonpermuted luciferase biosensors binding cyclic nucleotides |
| EP2281046B1 (en) | 2008-05-19 | 2012-01-25 | Promega Corporation | LUCIFERASE BIOSENSORS FOR cAMP |
| EP3508570B1 (en) | 2010-05-11 | 2020-07-22 | Promega Corporation | Mutant protease biosensors with enhanced detection characteristics |
| US9290794B2 (en) * | 2010-05-11 | 2016-03-22 | Promega Corporation | Mutant protease biosensors with enhanced detection characteristics |
| GB202108355D0 (en) * | 2021-06-11 | 2021-07-28 | Univ Edinburgh | Granzyme B detection |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6602677B1 (en) * | 1997-09-19 | 2003-08-05 | Promega Corporation | Thermostable luciferases and methods of production |
| GB9823468D0 (en) * | 1998-10-28 | 1998-12-23 | Secr Defence | Novel enzyme |
| US7879540B1 (en) | 2000-08-24 | 2011-02-01 | Promega Corporation | Synthetic nucleic acid molecule compositions and methods of preparation |
| US7927871B2 (en) * | 2002-01-29 | 2011-04-19 | Oncoimmunin, Inc. | Visualization and quantitation of cellular cytotoxicity using cell-permeable fluorogenic protease substrates and caspase activity indicator markers |
| JP4485470B2 (ja) * | 2002-12-23 | 2010-06-23 | プロメガ コーポレイション | ルシフェラーゼ酵素活性を保護するための方法及びキット |
| EP2308978B8 (en) | 2003-10-10 | 2015-04-08 | Promega Corporation | Luciferase biosensor |
| EP2004813B1 (en) * | 2006-04-03 | 2015-06-03 | Promega Corporation | Permuted and nonpermuted luciferase biosensors binding cyclic nucleotides |
| EP2154159A4 (en) * | 2007-05-09 | 2012-03-21 | Univ Tokyo | INDICATOR FOR ACTIVATED PROTEASE |
| EP3508570B1 (en) * | 2010-05-11 | 2020-07-22 | Promega Corporation | Mutant protease biosensors with enhanced detection characteristics |
-
2012
- 2012-11-12 EP EP12791055.2A patent/EP2776573A1/en not_active Withdrawn
- 2012-11-12 JP JP2014541370A patent/JP6421977B2/ja active Active
- 2012-11-12 WO PCT/US2012/064675 patent/WO2013071237A1/en not_active Ceased
- 2012-11-12 EP EP18205893.3A patent/EP3467119B1/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP3467119A1 (en) | 2019-04-10 |
| EP2776573A1 (en) | 2014-09-17 |
| EP3467119B1 (en) | 2022-02-23 |
| JP2015501636A (ja) | 2015-01-19 |
| WO2013071237A1 (en) | 2013-05-16 |
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