JP6293403B2 - Wrinkle inhibitor, epidermis moisture content inhibitor, epidermal thickening inhibitor, food and drink, and skin cosmetics - Google Patents

Description

  The present invention relates to a wrinkle inhibitor, an oral preparation and a skin cosmetic.

The formation of wrinkles is due to endogenous aging caused by the lifespan of cells affected by genetic factors and extrinsic aging caused by living environments such as ultraviolet rays and atmospheric chemicals. It is known to be caused.
Among these, exogenous aging due to UV exposure is said to have the greatest effect on wrinkle formation. In addition, wrinkle formation may be caused by a decrease in the amount of water in the epidermis caused by exposure of the skin to ultraviolet rays or an increase in the thickness of the epidermis.

  In order to suppress the formation of such wrinkles, for example, an external preparation for skin containing ceramide effective for moisturizing and atopy has been proposed (for example, see Patent Document 1). In addition, it has been proposed that konjac-derived glucosylceramide can prevent skin aging by causing epidermal cells to grow (see, for example, Patent Document 2). However, these proposals have a problem that they are insufficient for suppressing wrinkles caused by a decrease in the amount of water in the epidermis caused by exposure of the skin to ultraviolet rays or thickening of the epidermis.

  On the other hand, pineapple-derived glucosylceramide, which has sugar bound to ceramide, is known to have a moisturizing effect, but it suppresses wrinkles caused by skin moisture reduction and skin thickening caused by UV exposure to the skin. About is not known at all.

  Therefore, wrinkle suppressants, oral oral preparations and skin cosmetics that are excellent in safety and are excellent in suppressing wrinkles due to UV exposure, reduction in epidermal water content, and epidermal thickening have not been provided yet. It is.

JP 2003-160462 A JP 2011-195550 A

  An object of the present invention is to solve the conventional problems and achieve the following objects. That is, the present invention aims to provide a wrinkle inhibitor, an oral preparation, and a skin cosmetic that are excellent in safety and excellent in suppressing wrinkles caused by exposure to ultraviolet rays, lowering of the epidermal water content, and thickening of the epidermis. And

  In order to solve the above-mentioned problems, the present inventors have made extensive studies and as a result, obtained the following findings. That is, the present inventors have found that a wrinkle inhibitor containing glucosylceramide extracted from pineapple is excellent in safety and excellent in suppressing wrinkles, epidermal water content reduction, and epidermal thickening caused by ultraviolet exposure.

The present invention is based on the above findings by the present inventors, and means for solving the above problems are as follows. That is,
<1> A wrinkle inhibitor characterized by containing glucosylceramide extracted from pineapple.
<2> The wrinkle inhibitor according to <1>, which suppresses wrinkles due to ultraviolet exposure.
<3> The wrinkle inhibitor according to any one of <1> to <2>, which suppresses a decrease in the amount of water in the epidermis caused by exposure to ultraviolet rays.
<4> The wrinkle inhibitor according to any one of <1> to <2>, which suppresses thickening of the skin caused by ultraviolet exposure.
<5> An oral preparation containing the wrinkle inhibitor according to any one of <1> to <4>.
<6> A skin cosmetic comprising the wrinkle inhibitor according to any one of <1> to <4>.
<7> UV exposure-induced wrinkle inhibitor, epidermis moisture content inhibitor, epidermis thickening inhibitor, oral oral preparation for wrinkle control or wrinkle control characterized by containing glucosylceramide extracted from pineapple It is a skin cosmetic.

  According to the present invention, the conventional problems can be solved, the object can be achieved, the safety is excellent, and it is excellent in suppressing wrinkles, epidermal water content reduction, and epidermal thickening caused by ultraviolet exposure. A wrinkle suppressant, an oral preparation and a skin cosmetic can be provided.

FIG. 1 is a diagram showing the change in feed intake during the test period. FIG. 2 is a diagram showing a change in the weight of the rat during the feeding period. 3A is a microscope image of the back skin in Reference Example 1. FIG. 3B is a microscope image of the back skin in Reference Example 2. FIG. 3C is a microscope image of the back skin in Example 1. FIG. 3D is a microscope image of the back skin in Example 2. FIG. 4A is a replica analysis image of the back skin in Reference Example 1. FIG. 4B is a replica analysis image of the back skin in Reference Example 2. FIG. 4C is a replica analysis image of the back skin in Example 1. FIG. 4D is a replica analysis image of the back skin in Example 2. FIG. FIG. 5A is a diagram showing a comparison of the wrinkle volume ratio in each test group. FIG. 5B is a diagram showing a comparison of the wrinkle area ratio in each test group. FIG. 5C is a diagram showing a comparison of the number of wrinkles in each test group. FIG. 6 is a diagram showing a comparison of the amount of skin water in each test group. 7A is a HE-stained image of the back skin in Reference Example 1. FIG. FIG. 7B is an HE-stained image of the back skin in Reference Example 2. FIG. 7C is an HE-stained image of the back skin in Example 1. FIG. 7D is an HE-stained image of the back skin in Example 2.

(Wrinkle inhibitor)
The wrinkle inhibitor of the present invention contains glucosylceramide extracted from pineapple, and further contains other components as necessary.

<Glucosylceramide extracted from pineapple>
There is no restriction | limiting in particular as glucosylceramide extracted from the said pineapple, Although it can select suitably according to the objective, The mixture of the compound represented by either of the following general formula (1)-(2) is preferable. .
However, in the said General formula (1), m is a repeating unit number and represents the integer of 10-20.
However, in said general formula (2), n is a repeating unit number and represents the integer of 20-30.

There is no restriction | limiting in particular as a compound represented by the said General formula (1), Although it can select suitably according to the objective, The compound represented by either of following structural formula (1)-(2) is shown. preferable.

There is no restriction | limiting in particular as a compound represented by the said General formula (2), Although it can select suitably according to the objective, The compound represented by either of following Structural formula (3)-(5) is shown. preferable.

  In the glucosylceramide extracted from the pineapple, there is no particular limitation as a method for analyzing that the general formulas (1) to (2) and the structural formulas (1) to (5) are contained, The method can be appropriately selected according to the purpose. For example, a method of analyzing by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (Matrix Assisted Laser Desorption / Time of Flight Mass Spectrometry; MALDI-TOFMS) is used. Can be mentioned.

<< Method for producing glucosylceramide extracted from pineapple >>
There is no restriction | limiting in particular as a manufacturing method of the glucosylceramide extracted from the said pineapple, According to the objective, it can select suitably, For example, the process of preparing a pineapple, an extraction process, a precipitation filtration process, and redissolving It includes a process and a purification process, and further includes other processes such as a filtration process and a concentration process as necessary.

-Process for preparing pineapple-
The pineapple is an evergreen perennial plant originating in tropical America, and its scientific name is Ananas comosus . There is no restriction | limiting in particular as a site | part of the said pineapple, It can select suitably according to the objective, For example, pineapple edible parts, such as a fruit part, a fruit core part (core part), a fruit skin part, a fruit juice part, a fiber part, etc. Pineapple inedible parts such as leaves, stems, flowers, roots, etc .; Among these, a pineapple edible part is preferable in terms of abundant eating experience, and a residue after squeezing the pineapple edible part, that is, a fiber part (pineapple pulp part) remaining after collecting the fruit juice part of pineapple is more preferable.

  There is no restriction | limiting in particular as a process of preparing the said pineapple, Although it can select suitably according to the objective, After extract | collecting a plant, the method of wash | cleaning, drying, grind | pulverizing, pressing and preparing is preferable. The drying may be performed in the sun or may be performed using a commonly used dryer or the like.

-Extraction process-
The extraction step is a step of obtaining an extract by extracting the pineapple prepared in the step of preparing the pineapple with an 80% by volume to 100% by volume aqueous ethanol solution.
Through the extraction step, the fat-soluble component contained in the pineapple can be eluted into the aqueous ethanol solution to obtain an extract containing the fat-soluble component. The aqueous ethanol solution is a solvent that can be used for foods and drinks, cosmetics, and the like, and is preferable in terms of excellent safety.

  There is no restriction | limiting in particular as said extraction method, Although it can select suitably according to the objective, The said pineapple is thrown into the processing tank filled with 80 volume%-100 volume% ethanol aqueous solution, and as needed. A method of heating and extracting with a reflux extractor while stirring appropriately and filtering while hot to elute the fat-soluble component into the aqueous ethanol solution is preferred.

  The extraction conditions are not particularly limited and may be appropriately selected according to the purpose. The amount of the ethanol aqueous solution with respect to the pineapple is preferably 5 to 20 times (mass ratio), and the extraction time is Is preferably 1 hour to 3 hours, and the extraction temperature is preferably 20 ° C. to 95 ° C. The water used for the aqueous ethanol solution (extraction solvent) is pure water, tap water, well water, mineral water, mineral water, hot spring In addition to water, spring water, fresh water, etc., water subjected to various treatments (for example, purification, heating, sterilization, filtration, ion exchange, adjustment of osmotic pressure, buffering, etc.) is preferable.

  The ethanol concentration of the aqueous ethanol solution (extraction solvent) at the time of extraction is not particularly limited as long as it is 80% by volume to 100% by volume, and can be appropriately selected according to the purpose. % Is preferred. If the concentration is less than 80% by volume, glucosylceramide may not be extracted efficiently. On the other hand, the preferable range is advantageous in that glucosylceramide can be easily extracted.

-Precipitation filtration step-
The precipitation filtration step is a step in which the extract is diluted and filtered until an ethanol concentration of 25% by volume to 35% by volume is obtained to obtain a precipitated glucosylceramide residue. In the precipitation filtration step, glucosylceramide is precipitated in the residue (filtered matter) by diluting the extract (diluted to a volume of 25% to 35% by volume ethanol) and filtering. Sugars and the like are dissolved in.

There is no restriction | limiting in particular as said dilution method, According to the objective, it can select suitably, For example, the method of adding water (dilution solvent) to the said extract and diluting etc. are mentioned.
There is no restriction | limiting in particular as said dilution conditions, Although it can select suitably according to the objective, As the temperature of the said water, 30 to 60 degreeC is preferable, As said water (dilution solvent), the said ethanol aqueous solution The thing similar to the water used by (extraction solvent) is preferable.

  The ethanol concentration of the aqueous ethanol solution (diluted solution) after the dilution is not particularly limited as long as it is 25% by volume to 35% by volume, and can be appropriately selected according to the purpose. When the concentration is less than 25% by volume, filterability may be deteriorated, and when it exceeds 35% by volume, glucosylceramide may be dissolved. On the other hand, the preferable range is advantageous in that the filterability is good with glucosylceramide precipitated. There is no restriction | limiting in particular as said ethanol aqueous solution (dilution liquid), Although it can select suitably according to the objective, It is preferable to stir from the point which can homogenize the said ethanol aqueous solution (dilution liquid).

There is no restriction | limiting in particular as said precipitation method, According to the objective, it can select suitably.
The deposition conditions are not particularly limited and may be appropriately selected according to the purpose. The solution temperature during the deposition is preferably 30 ° C. to 60 ° C., and the deposition time is 30 minutes or more. preferable.

There is no restriction | limiting in particular as said filtration method, According to the objective, it can select suitably, For example, the filtration method by natural filtration, reduced pressure filtration, pressure filtration, centrifugal filtration etc. are mentioned.
The filtration conditions are not particularly limited and may be appropriately selected depending on the purpose. However, the filter medium used for the filtration is preferably cellulose, a glass fiber filter, a membrane filter, diatomaceous earth, or the like, obtained by the filtration. The residue is preferably washed with an aqueous ethanol solution of 35% by volume or less in that saccharides can be removed and glucosylceramide does not dissolve.

-Re-dissolution process-
The redissolving step is a step of redissolving the residue in a 70% by volume to 100% by volume ethanol aqueous solution to obtain a redissolved solution. Impurities contained in the residue are removed by the re-dissolution step.

  The re-dissolution method is not particularly limited and may be appropriately selected depending on the purpose. For example, the residue is suspended in the ethanol aqueous solution (solvent for re-dissolution) and re-dissolved with stirring. Etc.

  The re-dissolution condition is not particularly limited and can be appropriately selected according to the purpose. The amount of the aqueous ethanol solution is preferably 5 to 20 times (mass ratio) with respect to the residue. The remelting time is preferably 1 hour to 3 hours, and the remelting temperature is preferably 20 ° C to 95 ° C. As the redissolved solution obtained by the re-dissolution, a solution obtained by combining the filtrate obtained by filtration and the filtrate of the washing solution may be used as the re-dissolved solution. As a method for filtering the redissolved solution, a method similar to the method for filtering the diluted solution can be used.

  The ethanol concentration of the aqueous ethanol solution (solvent for re-dissolution) at the time of re-dissolution is not particularly limited as long as it is 70% by volume to 100% by volume, and can be appropriately selected. Volume% is preferred. If the concentration is less than 70% by volume, glucosylceramide may not dissolve. On the other hand, the preferable range is advantageous in that the solubility of glucosylceramide is good. There is no restriction | limiting in particular as said ethanol aqueous solution (solvent for re-dissolving), Although it can select suitably according to the objective, 0.1 mol / L-1.0 mol / L alkali (for example, potassium hydroxide) , Sodium hydroxide, etc.).

-Purification step-
The said refinement | purification process is a process of eluting with 90 volume%-100 volume% ethanol aqueous solution, after making the resolubilization liquid obtained in the said remelting process adsorb | suck to a synthetic adsorbent. Through the purification step, an extract containing glucosylceramide can be purified.

  The purification method is not particularly limited and may be appropriately selected depending on the intended purpose. For example, after passing the re-dissolved solution through a synthetic adsorbent by a batch method, 90% by volume to 100% by volume ethanol Examples include a method of elution with an aqueous solution and a method of purifying by eluting with a 90% by volume to 100% by volume of ethanol aqueous solution after passing the re-dissolved solution through a synthetic adsorbent by a column method. Among these, purification using a column method is preferable.

  The purification conditions are not particularly limited and may be appropriately selected depending on the purpose. The amount of the synthetic adsorbent is 1/10 to 1/2 times the amount of the re-dissolved solution ( (Volume ratio) is preferable, and the flow rate of the re-dissolved liquid in the column method is preferably 1-fold amount / hour to 3-fold amount / hour of the amount of the synthetic adsorbent. ° C is preferred. The re-dissolved solution is not particularly limited and may be appropriately selected depending on the intended purpose. However, in order to remove components not adsorbed on the synthetic adsorbent, the synthesis is performed after passing through and before elution. It is preferable to wash with 75% to 85% by volume of an aqueous ethanol solution that is twice to 10 times the volume of adsorbent (volume ratio). Examples of the synthetic adsorbent include styrene-divinylbenzene synthetic adsorbent and methacrylic synthetic adsorbent. Among these, a styrene-divinylbenzene synthetic adsorbent is preferable. As the styrene-divinylbenzene synthetic adsorbent, commercially available products can be used, and examples of the commercially available products include Diaion HP20 and Diaion SP825 (both manufactured by Mitsubishi Chemical Corporation).

  The ethanol concentration of the aqueous ethanol solution (eluent) at the time of purification is not particularly limited and may be appropriately selected depending on the intended purpose, but is 5 times the amount of the synthetic adsorbent in that glucosylceramide is easily eluted. 90 volume%-100 volume% of 10 times amount (volume ratio) is preferable.

-Other processes-
There is no restriction | limiting in particular as said other process, According to the objective, it can select suitably, For example, a filtration process, a concentration process, etc. are mentioned.

  The filtration step is a step of filtering the extract containing the fat-soluble component obtained in the extraction step before diluting the extract in the precipitation filtration step. By the filtration step, pineapple residues and insoluble components after extraction can be removed. Examples of the filtering method include filtration using a cloth, natural filtration, vacuum filtration, pressure filtration, centrifugal filtration, and the like.

  The concentration step is a step of concentrating a solution such as the extract obtained in the extraction step, the redissolved solution obtained in the re-dissolution step, and the purified solution obtained in the purification step. The concentration step can increase the concentration of the target glucosylceramide, and can reduce the amount of solution to be handled and the amount of waste liquid. The concentration method is not particularly limited, and a known concentration method can be appropriately selected according to the purpose. Examples thereof include atmospheric concentration for distillation in the atmosphere, concentration under reduced pressure using an evaporator, and the like. .

<< Content of glucosylceramide extracted from pineapple >>
There is no restriction | limiting in particular as content in the said wrinkle inhibitor of the glucosylceramide extracted from the said pineapple, Although it can select suitably according to the objective, 0.0001 mass%-20 mass% are preferable, and 0. 0001% by mass to 10% by mass is more preferable, 0.001% by mass to 1% by mass is more preferable, and 0.005% by mass to 0.015% by mass is particularly preferable in that it is excellent in suppression of wrinkle and skin water content reduction. Preferably, 0.05 mass% to 0.15 mass% is particularly preferable in terms of excellent suppression of epidermis thickening.

<Other ingredients>
The other components are not particularly limited and may be appropriately selected depending on the intended purpose. For example, astringents, bactericides, antibacterial agents, cell activators, excipients, moisture-proofing agents, preservatives, reinforcing agents , Thickeners, emulsifiers, antioxidants, sweeteners, acidulants, seasonings, colorants, whitening agents, moisturizers, oily ingredients, UV absorbers, fats and oils, waxes, hydrocarbons, fatty acids, alcohol , Esters, water, surfactants, fragrances, nutrients and the like.

<Usage>
There is no restriction | limiting in particular as usage of the said wrinkle inhibitor, According to the objective, it can select suitably, For example, administration forms, such as oral and parenteral, are mentioned, You may use externally. Among these, oral administration is preferable in that it is excellent in suppressing wrinkles caused by exposure to ultraviolet rays, lowering of the epidermal water content, and epidermal thickening.

<Dosage form>
The dosage form of the wrinkle inhibitor is not particularly limited and may be appropriately selected depending on the intended purpose. For example, oral administration agents such as tablets, powders, capsules, granules, extracts and syrups; injections And parenteral administration agents such as ointments, drops, suppositories; and external preparations such as ointments, creams, emulsions, lotions, packs, bath preparations and the like.

<Application>
The wrinkle suppressant of the present invention is excellent in safety and is excellent in suppressing wrinkles, epidermal water content reduction, and epidermal thickening caused by UV exposure, and therefore, epidermis water content reduction inhibitor, epidermal thickening resulting from UV exposure. It can be suitably used as an inhibitor, a pharmaceutical agent relating to cell damage, and a safe preventive agent relating to these diseases. Moreover, since it has been confirmed that the wrinkle inhibitor of the present invention is not digested in the digestive tract, it can be suitably used as a food or drink such as a beauty food or drink or a health food or drink.
In addition, although the wrinkle inhibitor of this invention is applied suitably with respect to a human, as long as each effect is show | played, it can also apply to animals other than a human.

  The wrinkle suppressant of the present invention is excellent in safety and excellent in suppressing wrinkles, epidermal water content reduction, and epidermal thickening caused by exposure to ultraviolet rays. For example, for use in the skin cosmetic of the present invention described later. Particularly preferred.

(Oral for oral use)
The oral preparation of the present invention contains the above-described wrinkle inhibitor of the present invention, and further contains the other components as necessary.

  There is no restriction | limiting in particular as a dosage form of the said oral internal preparation, It can select suitably from various uses, For example, a tablet, a capsule, a granule, a powder, a liquid agent etc. are mentioned.

  The content of the wrinkle inhibitor in the oral preparation is not particularly limited and can be appropriately adjusted according to the type of oral preparation and physiological activity of the extract, but is extracted from the pineapple. In terms of the amount of glucosylceramide, 0.0001% by mass to 20% by mass is preferable, 0.0001% by mass to 10% by mass is more preferable, and 0.01% by mass to 10% by mass is particularly preferable.

The oral preparation for oral use of the present invention is not only excellent in the wrinkle-suppressing effect, epidermal water content reduction-suppressing effect, and epidermal thickening-inhibiting effect caused by exposure to ultraviolet rays, but is also excellent in safety.
In addition, although the oral internal preparation of this invention is applied suitably with respect to a human, as long as each effect is show | played, it can also be applied with respect to animals other than a human.

(Skin cosmetic)
The skin cosmetic of the present invention contains the above-described wrinkle inhibitor of the present invention, and further contains the other components as necessary.

  The use of the skin cosmetic is not particularly limited and can be appropriately selected from various uses. Examples thereof include ointments, creams, emulsions, lotions, packs, jellies, lip balms, lipsticks, bath preparations, and astringents. It is done.

  The content of the wrinkle inhibitor in the skin cosmetic is not particularly limited and can be appropriately adjusted according to the type of skin cosmetic, physiological activity of the extract, etc., but glucosyl extracted from the pineapple In terms of the amount of ceramide, 0.0001% by mass to 20% by mass is preferable, 0.0001% by mass to 10% by mass is more preferable, and 0.01% by mass to 10% by mass is particularly preferable.

The skin cosmetic of the present invention is not only excellent in the wrinkle suppressing action, the epidermal water content lowering suppressing action, and the epidermal thickening suppressing action caused by UV exposure, but also in safety.
In addition, although the skin cosmetics of this invention are suitably applied with respect to a human, as long as each effect is show | played, it can also apply to animals other than a human.

  EXAMPLES Hereinafter, although an Example and a compounding example are shown and this invention is demonstrated more concretely, this invention is not restrict | limited to these.

[Test substance]
As a test substance, glucosylceramide (manufactured by Maruzen Pharmaceutical Co., Ltd.) extracted from pineapple was used. The glucosylceramide extracted from the pineapple contains the general formulas (1) to (2) and the structural formulas (1) to (5). It has been confirmed by type mass spectrometry (Matrix Assisted Laser Desorption / Ionization-Time of Flight Mass Spectrometry; MALDI-TOFMS). In this test, as a glucosylceramide, a mixed diet blended with mouse feed (Lab MR Stock, manufactured by Nippon Agricultural Industry Co., Ltd.) at a ratio of 0.01% by mass and 0.1% by mass is prepared for free consumption. Ingested.

[Test animals]
After purchasing a 5-week-old female hairless mouse (Hos: HR-1, Shimizu Experimental Materials Co., Ltd., Kyoto City), fully quarantine and observing the general condition and measuring the body weight, Selected and used at 6 weeks of age.

[Raising conditions]
Breeding: Temperature: 22 ± 3 ° C, Humidity: 55 ± 15%, Ventilation: Always fresh, lighting 12 hours / day (6 am to 6 pm), 4 in each plastic housing cage Accommodated. In addition, drinking water was taken freely with an automatic water supply device.

[Composition of test group]
The four-group configuration shown in Table 1 below was adopted.

[UV irradiation conditions]
Six-week-old hairless mice (n = 8) were assigned to each test group. The back of hairless mice in each test group was irradiated with ultraviolet rays, and at the same time, the “feed” shown in Table 1 or “a mixed feed containing a test substance in the feed” was freely ingested. Food intake was measured daily and body weight was measured and recorded weekly. Ultraviolet irradiation was performed as shown in Table 2 below, and was performed three times a week using an ultraviolet lamp (FL20S-E lamp, manufactured by Toshiba Corporation). The total irradiation amount of ultraviolet rays was 1,278 mJ / cm ² .

[Statistical processing]
To examine the effectiveness of the test substance, Dunnett's multiple test was performed, and if the p-value was less than 0.05, it was determined to be “significant”.

[Results of feed intake and test substance intake]
The feed intake during the test period is shown in FIG. 1, the average food intake and the test substance intake during the test period are shown in Table 3, and the influence of the test substance on the body weight is shown in FIG. There was no significant difference in food intake in any of the test groups. Therefore, it was judged that there was no effect on the food intake by glucosylceramide extracted from pineapple. In addition, all the test groups confirmed a steady increase in weight.

(Test Example 1: Wrinkle suppression test)
On the day after the final irradiation end date (after 5 weeks), the back skin of the hairless mouse was photographed using a microscope. The microscope images obtained by photographing the back skin of the hairless mouse in each test group are shown in FIGS.
From these results, formation of wrinkles was observed in the test group that was irradiated with ultraviolet rays for 5 weeks, compared to the test group that was not irradiated with ultraviolet rays. At this time, it was found that the test group of Example 1 exhibited the most wrinkle-inhibiting action among the test groups subjected to ultraviolet irradiation.

(Test Example 2: Wrinkle suppression action test)
On the day after the last irradiation end date (after 5 weeks), a replica of the back skin of hairless mice was prepared, and then wrinkle analysis (wrinkle volume ratio, wrinkle area ratio) using ASA-3RXD (manufactured by Asahi Biomed) And the number of wrinkles). Replica analysis images of the back skin of hairless mice in each test group are shown in FIGS. 4A to D, and the results of wrinkle analysis using replicas are shown in FIGS.
From these results, formation of wrinkles was observed in the test group that was irradiated with ultraviolet rays for 5 weeks, compared to the test group that was not irradiated with ultraviolet rays. At this time, it was found that, among the test groups subjected to ultraviolet irradiation, the test group of Example 1 exhibited the lowest wrinkle volume ratio, wrinkle area ratio, and number of wrinkles with respect to Reference Example 2, and exhibited a wrinkle suppressing action. .

(Test Example 3: Skin water content lowering suppression test)
On the day following the end of the last irradiation (after 5 weeks), the moisture content of hairless mice was measured without anesthesia. The measurement was performed using a skin surface moisture measuring device (CORNEOMETER). The measurement results of the moisture content of the hairless mice in each test group are shown in FIG.
From these results, a significant decrease in the amount of water was observed in the test group that had been irradiated with ultraviolet rays for 5 weeks compared to the test group that had not been irradiated with ultraviolet rays. At this time, it was found that, among the test groups that were irradiated with ultraviolet rays, the test group of Example 1 exhibited the smallest reduction in epidermal water content and exhibited the effect of suppressing the reduction in epidermal water content.

(Test Example 4: Epidermis thickening inhibitory action test)
The back skin of hairless mice was removed on the day following the end of the last irradiation (after 5 weeks). After excision, formalin fixation was performed, and histological examination by HE staining was performed. The HE-stained images of the back skin of hairless mice in each test group are shown in FIGS.
From these results, in the test group that was irradiated with ultraviolet rays for 5 weeks, epidermal thickening and increased keratinization were observed compared to the test group that was not irradiated with ultraviolet rays. At this time, it turned out that the test group of Example 2 exhibits the epidermal thickening inhibitory effect most among the test groups which performed ultraviolet irradiation.

(Formulation example 1: emulsion)
An emulsion having the following composition was produced by a conventional method. It was found that the emulsion of Formulation Example 1 was excellent in safety and excellent in suppressing wrinkles, epidermal water content reduction, and epidermal thickening caused by ultraviolet exposure.
-Composition-
・ Glucosylceramide extracted from pineapple (Maruzen Pharmaceutical Co., Ltd.) 0.2g
・ Cetyl alcohol 0.5g
・ Beeswax 2g
・ POE (10) monooleate 1g
・ Glycerin monostearate 1g
・ Sodium hyaluronate 0.1g
・ Propylene glycol 5g
・ Ethanol 3g
・ Ethylbaraben 0.3g
・ Perfume 0.03g
・ Remaining amount of purified water (total volume is 100 mL)

(Formulation Example 2: Pack)
A pack having the following composition was produced by a conventional method. The pack of Formulation Example 2 was found to be excellent in safety and excellent in suppressing wrinkles, epidermis water content reduction, and epidermal thickening caused by UV exposure.
-Composition-
・ Glucosylceramide extracted from pineapple (manufactured by Maruzen Pharmaceutical Co., Ltd.) 0.3g
・ Polyvinyl alcohol 15g
・ Polyethylene glycol 3g
・ Propylene glycol 7g
・ Ethanol 10g
・ Methylparaben 0.05g
・ Fragrance 0.05g
・ Remaining amount of purified water (total volume is 100 mL)

(Formulation Example 3: Cream)
A cream having the following composition was produced by a conventional method. The pack of Formulation Example 3 was found to be excellent in safety and excellent in suppressing wrinkles, epidermal water content reduction, and epidermal thickening caused by exposure to ultraviolet rays.
-Composition-
・ Glucosylceramide extracted from pineapple (Maruzen Pharmaceutical Co., Ltd.) 0.8g
・ Cetostearyl alcohol 3.5g
・ Squalane 40g
・ Bees 3g
・ Reduced lanolin 5g
・ Ethylbaraben 0.3g
・ Polyoxyethylene 2g
・ Stearic acid monoglyceride 2g
・ 1,3-butylene glycol 5g
・ Perfume 0.03g
・ Glycerin 5g

(Formulation example 4: tablets for oral use)
Tablets for oral use having the following composition were produced by a conventional method. It turned out that the tablet of the combination example 4 is excellent in safety | security, and is excellent in suppression of the wrinkles resulting from an ultraviolet exposure, epidermis moisture fall, and epidermal thickening.
-Composition-
・ Glucosylceramide extracted from pineapple (Maruzen Pharmaceutical Co., Ltd.) 1.0g
・ Powdered sugar (sucrose) 178g
・ Sorbit 10g
・ Glycerin fatty acid ester 11g

(Formulation example 5: Collagen-containing drink)
A collagen-containing drink, which is an oral oral medicine having the following composition, was produced by a conventional method. It was found that the collagen-containing drink of Formulation Example 5 was excellent in safety and excellent in suppressing wrinkles, epidermal water content reduction, and epidermal thickening caused by UV exposure.
・ Vitamin C 1g
・ Collagen 5g
・ Hyaluronic acid 5mg
・ Flavoring proper amount ・ Sour seasoning appropriate amount ・ Water balance (the total amount is 100 mL)

  The wrinkle-suppressing agent of the present invention is excellent in safety, and is excellent in suppressing wrinkles caused by exposure to ultraviolet rays, reduction in the amount of moisture in the epidermis, and thickening of the epidermis. It can be suitably used for skin cosmetics (wrinkle-suppressing cosmetics, whitening cosmetics, moisturizing cosmetics, anti-aging cosmetics), cosmetic raw materials, pharmaceuticals, pharmaceutical raw materials and the like.

Claims (7)

An exogenous wrinkle inhibitor caused by ultraviolet exposure, which contains any glucosylceramide extracted from pineapple, represented by the following structural formulas (1) to (5).
  The wrinkle inhibitor according to claim 1, which suppresses a decrease in the amount of water in the epidermis caused by exposure to ultraviolet rays.   The wrinkle inhibitor according to claim 1, which suppresses thickening of the epidermis caused by exposure to ultraviolet rays. The epidermis water content fall inhibitor resulting from ultraviolet exposure containing all the glucosylceramide extracted from pineapple represented by following Structural formula (1)-(5).
An epidermis thickening inhibitor resulting from ultraviolet exposure, which contains any of glucosylceramides extracted from pineapple, represented by the following structural formulas (1) to (5).
All of the glucosylceramides extracted from pineapples represented by the following structural formulas (1) to (5) are used to suppress exogenous wrinkles caused by UV exposure, and to suppress the reduction of epidermal water content caused by UV exposure. Food or drink for suppressing skin thickening caused by exposure to UV rays or exposure to ultraviolet rays.
All of the glucosylceramides extracted from pineapples represented by the following structural formulas (1) to (5) are used to suppress exogenous wrinkles caused by UV exposure, and to suppress the reduction of epidermal water content caused by UV exposure. Skin cosmetic for suppressing skin thickening due to use or exposure to ultraviolet rays.