JP6292855B2 - Liquid disinfectant composition for hard surfaces - Google Patents

Description

  The present invention relates to a liquid bactericidal composition for hard surfaces.

  In the restaurant industry, which provides food to an unspecified number of people, it is possible to provide consumers with safety and security in all aspects of food provision, food processing and cooking, and food distribution, as well as providing food. It has become an industry mission. Therefore, it is necessary to increase the degree of hygiene on various hard surfaces used in food production such as food processing equipment, cooking utensils, and kitchen equipment.

  As a guideline for hygiene management in the field of food production, there is a “Manufacturing Manual for Mass Cooking Facilities” which is a guideline established by the Ministry of Health, Labor and Welfare. The method uses various chemicals such as alcohol preparations and sodium hypochlorite and requires complicated work, which may cause equipment and time burdens. There is one aspect that does not match the actual situation. If accurate hygiene management work is not performed, there is a concern that disadvantages due to bacteria such as food poisoning and food deterioration may occur.

  Patent Document 1 discloses protamine, polylysine, and citrus plant as a sterilizing composition that can be used for sterilization, disinfection and washing of food, kitchen, kitchen utensils, tableware, cloths, fingers, skin and the like. Disinfecting compositions containing seed extract components are disclosed.

  Patent Document 2 discloses a solution for cleaning and disinfecting contact lenses, which contains polylysine and / or protamine and an enzyme that acts on dirt on the contact lens surface.

  Patent Document 3 discloses a contact lens care solution containing a fatty acid ester of sucrose which is soluble in water.

  Patent Document 4 discloses a sterilizing detergent containing a basic protein obtained from fish larvae or salts thereof used for hygiene such as Oshibori.

JP 2002-322009 A JP 2001-228444 A JP 2000-292754 A JP-A-64-71805

  In the composition of patent documents 1-4, sufficient sterilization effect is not acquired as a sterilization washing agent of hard surfaces, such as food processing equipment, a cooking utensil, and kitchen equipment, and reliable sterilization cannot be performed.

  The problem to be solved by the present invention is to provide a hard surface liquid disinfectant composition that can sterilize hard surfaces of food processing equipment, cooking utensils or kitchen equipment safely, simply and reliably. .

The present invention includes the following components (a), (b) and (c):
(A) Ingredient: Polylysine (b) Ingredient: Protamine (c) Ingredient: It relates to a liquid fungicide composition for hard surface containing sucrose fatty acid ester and water.

  Moreover, this invention relates to the sterilization method of a hard surface which applies the liquid germicide composition for hard surfaces of the said invention to a hard surface.

  ADVANTAGE OF THE INVENTION According to this invention, the liquid detergent composition for hard surfaces which can sterilize hard surfaces, such as a food processing equipment, a cooking utensil, or kitchen equipment, safely and easily and reliably is provided. Moreover, according to this invention, the sanitary degree of a hard surface can be maintained in a high state.

  The present invention comprises (a) component: polylysine, (b) component: protamine, (c) component: sucrose fatty acid ester, and water, so that hard surfaces such as food processing equipment, cooking utensils or kitchen equipment can be safely The present invention provides a hard surface liquid detergent composition that can be sterilized easily and reliably.

<(A) component>
Examples of polylysine include polyamino acids obtained by polymerizing L-lysine, which is an essential amino acid, on a straight chain, and α-polylysine produced by chemical synthesis and ε-polylysine produced by fermentation. The polylysine as component (a) is preferably ε-polylysine from the viewpoint of bactericidal properties. The molecular weight of the polylysine as component (a) is preferably 3,000 or more, more preferably 4,000 or more, and preferably 10,000 or less, more preferably 8,000 or less, from the viewpoint of bactericidal properties. More preferably, it is 6,000 or less, More preferably, it is 5,000 or less. This molecular weight is a weight average molecular weight measured by GPC-LALLS method. In addition, the polylysine as the component (a) is preferably a polylysine having a quality level that can be used as a food additive from the viewpoint of safety.

<(B) component>
As protamine, one or more fish larvae obtained from a group consisting of about 50 or more fish including salmon, herring, rainbow trout and tuna are acid-treated, so that nucleo present in the sperm nucleus of each fish. Examples include protamine purified from protamine. The component (b), protamine, is preferably protamine obtained from salmon, herring, rainbow trout, tuna, and mullet, more preferably from salmon, from the viewpoint of bactericidal properties, safety, economy, and availability. Protamine obtained. The component (b), protamine, is preferably a protamine of a quality level that can be used as a food or food additive from the viewpoint of safety.

  Examples of the protamine (b) include free protamine and protamine salts. The component protamine (b) is preferably one or more selected from protamine salts, more preferably protamine sulfate and protamine hydrochloride from the viewpoints of bactericidal properties and compounding properties, and more preferably from the viewpoint of availability. Is protamine hydrochloride.

<(C) component>
Sucrose fatty acid ester synergistically increases bactericidal properties when used in combination with polylysine as component (a) and protamine as component (b).

  The carbon number of the fatty acid of the sucrose fatty acid ester is preferably 8 or more, more preferably 10 or more, more preferably 12 or more from the viewpoint of bactericidal properties, and preferably 14 or less from the viewpoint of solubility. is there.

  The sucrose fatty acid ester is preferably at least one selected from sucrose laurate and sucrose myristate, more preferably sucrose myristate, from the viewpoint of bactericidal properties and availability.

  Sucrose fatty acid esters include monoesters, diesters, triesters, tetraesters, pentaesters, hexaesters, heptaesters, and octaesters. Among these, from the viewpoint of bactericidal properties and availability, it is preferably one or more selected from monoesters, diesters and triesters, more preferably one or more selected from monoesters and diesters. More preferably, it is a monoester.

  The sucrose fatty acid ester has a monoester ratio of preferably 60% by mass or more, more preferably 65% by mass or more, more preferably 70% by mass or more, and more preferably 75% by mass or more from the viewpoint of bactericidal properties. And from a viewpoint of availability, Preferably it is 90 mass% or less, More preferably, it is 85 mass% or less.

  Moreover, the monoester ratio of sucrose laurate or sucrose myristate is preferably the above ratio from the viewpoint of bactericidal properties and availability.

<Liquid disinfectant composition>
The liquid germicide composition for hard surfaces of the present invention has a bactericidal property in which the total amount of component (a) and component (b) and the mass ratio of component (c) [(a) + (b)] / (c) In view of the above, preferably 0.001 or more, more preferably 0.005 or more, more preferably 0.008 or more, more preferably 0.015 or more, more preferably 0.1 or more, more preferably 0.15 or more. And preferably 25 or less, more preferably 10 or less, more preferably 5 or less, more preferably 4 or less, more preferably 2 or less, more preferably 0.3 or less.

  In the liquid germicide composition for hard surface of the present invention, the mass ratio of the component (a) to the component (b) [(a) / (b) is preferably 0.1 or more, more preferably from the viewpoint of bactericidal properties. Is 0.2 or more, more preferably 0.5 or more, more preferably 0.8 or more, and preferably 10 or less, more preferably 5 or less, more preferably 2 or less, more preferably 1.2 or less. It is.

  The total content of the component (a) and the component (b) in the hard surface liquid disinfectant composition of the present invention is preferably 0.001% by mass or more, more preferably 0.002% by mass, from the viewpoint of bactericidal properties. % Or more, more preferably 0.04% by mass or more, and preferably 1% by mass or less, more preferably 0.7% by mass or less, more preferably 0.5% by mass from the viewpoint of bactericidal properties and economy. % Or less, more preferably 0.3% by mass or less, more preferably 0.17% by mass or less, and more preferably 0.07% by mass or less.

  The content of the component (c) in the hard surface liquid disinfectant composition of the present invention is preferably 0.005% by mass or more, more preferably 0.02% by mass or more, more preferably from the viewpoint of bactericidal properties. 0.1 mass% or more, more preferably 0.18 mass% or more, and preferably 0.3 mass% or less, more preferably 0.25 mass% or less, more preferably 0.24 mass% or less, More preferably, it is 0.21 mass% or less.

  The liquid germicide composition for hard surfaces of the present invention contains water. Water is not particularly limited, but is preferably ion-exchanged water, distilled water, or sterilized water from the viewpoint of bactericidal properties. The water content is preferably 50% by mass or more, more preferably 70% by mass or more, more preferably 90% by mass or more, more preferably 95% by mass or more, more preferably from the viewpoint of economy. From the viewpoint of bactericidal properties, it is preferably less than 100% by mass, more preferably 99.9% by mass or less, and more preferably 99.8% by mass or less.

  The total content of component (a), component (b), component (c) and water in the liquid germicide composition for hard surface of the present invention is preferably 94% by mass or more, more preferably from the viewpoint of bactericidal properties. Is 95% by mass or more, more preferably 96% by mass or more, and from the viewpoint of economy, it is preferably 100% by mass or less, more preferably 99% by mass or less, more preferably 98% by mass or less.

  From the viewpoint of safety during use, the pH of the liquid sterilizing composition for hard surfaces of the present invention is preferably 5 or more, more preferably 6 or more, and preferably 9 or less, more preferably 8 or less.

  From the viewpoint of easy handling, the liquid germicide composition for hard surface of the present invention has a viscosity at 25 ° C. of preferably 0.1 mPa · s or more, more preferably 0.5 mPa · s or more, and preferably Is 10 mPa · s or less, more preferably 5 mPa · s or less. The viscosity can be measured using a BM viscometer (rotor NO. M1, rotation speed 60 r / min, 1 minute after the start of stirring).

  The liquid germicide composition for hard surfaces of the present invention is a surfactant other than the other components, for example, the component (a), the component (b), and the component (c), as long as the effects and safety of the invention are not impaired. , Fragrances, preservatives, pH adjusters, colorants and the like.

  The liquid germicide composition for hard surfaces of the present invention is preferably a uniform and transparent liquid from the viewpoints of ease of use and bactericidal effect.

  The method for producing the hard surface liquid disinfectant composition of the present invention is not particularly limited, but after first charging the water constituting the majority of the composition, and then adding each component in random order, It is preferable to stir until uniform and transparent. Then, it is desirable to measure pH and adjust it using an inorganic acid so that it may become predetermined | prescribed pH.

<Sterilization method>
In the sterilization method of the present invention, it is applied to the hard surface of the liquid sterilizer composition for hard surface of the present invention.

Although it does not specifically limit as a hard surface to apply, Hard surfaces, such as a food processing equipment, a cooking utensil, and kitchen equipment, are mentioned.
Examples of the food processing equipment include large equipment such as a meat slicer, a noodle making machine, a fryer, and a rice cooker. Moreover, the net conveyor or belt conveyor installed in these food processing facilities is mentioned.
Examples of cooking utensils include cutting boards, kitchen knives, and monkeys.
Kitchen equipment includes refrigerator door knobs, work tables, food storage showcases, and the like.

  The method for applying the hard surface liquid disinfectant composition of the present invention to a hard surface includes immersing the hard surface in the hard surface liquid detergent composition, spraying and applying the hard surface liquid detergent composition to the hard surface. It adheres by such as.

In the sterilization method of the present invention, the amount applied to the hard surface of the liquid sterilizer composition for hard surface is preferably a total of (a) component, (b) component, and (c) component from the viewpoint of bactericidal properties. Is 4 × 10 ⁻⁶ g / cm ² or more, more preferably 1 × 10 ⁻⁵ g / cm ² or more, and from the viewpoint of economy, preferably 1 × 10 ⁻² g / cm ² or less, more preferably The amount is 1 × 10 ⁻³ g / cm ² or less, more preferably 1 × 10 ⁻⁴ g / cm ² or less.

  In order to sufficiently exhibit the bactericidal effect of the component (a), the component (b) and the component (c), it is preferable that the bactericidal method of the present invention is allowed to stand for a certain time after the bactericidal composition is applied. That is, in the sterilization method of the present invention, it is preferable to maintain the state in which the components (a), (b) and (c) are attached to the hard surface after application to the hard surface. The time for which the component (a), the component (b), and the component (c) are attached to the hard surface is preferably 15 minutes or more, more preferably 20 minutes or more, and more preferably 50 from the viewpoint of bactericidal properties. From the viewpoint of economy, it is preferably 5 days or less, more preferably 3 days or less, more preferably 2 days or less, more preferably 24 hours or less, more preferably 20 hours or less, more preferably from the viewpoint of economy. It is 12 hours or less, more preferably 6 hours or less, more preferably 3 hours or less, more preferably 2 hours or less.

  (A) component, (b) component, (c) component of the liquid germicide composition for hard surfaces of this invention can use the product of the quality level which can be used as a food additive, respectively, In that case, adhesion More preferably, articles having a hard surface, for example, food processing equipment, cooking utensils or kitchen equipment can be used without rinsing, and excellent sterility can be maintained safely, simply and reliably. it can.

  Unless otherwise specified, the following experiments were performed in an environment of 25 ° C.

Examples 1-1 to 1-8, Comparative Examples 1-1 to 1-3
The composition described in Table 1 was prepared by the following method for preparing a chemical solution to obtain a chemical solution. In addition, a bacterial solution of S. aureus prepared by the following method was prepared. Using this bacterial solution, the bactericidal properties of each chemical solution were evaluated by the following bactericidal evaluation method. However, the standing time of (2) of the bactericidal evaluation method was 60 minutes (bactericidal 1).

Examples 2-1 to 2-5, comparative examples 2-1 to 2-3
The composition described in Table 2 was prepared by the following method for preparing a chemical solution to obtain a chemical solution. In addition, a bacterial solution of Acinetobacter prepared by the following method was prepared. Using this bacterial solution, the bactericidal properties of each chemical solution were evaluated by the following bactericidal evaluation method. However, the standing time of (2) of the bactericidal evaluation method was 60 minutes (bactericidal property 2).

Examples 3-1 to 3-8, comparative examples 3-1 to 3-4
The composition described in Table 3 was prepared by the following method for preparing a chemical solution to obtain a chemical solution. In addition, a bacterial solution of S. aureus prepared by the following method was prepared. Using this bacterial solution, the bactericidal properties of each chemical solution were evaluated by the following bactericidal evaluation method. However, the standing time of (2) in the bactericidal evaluation method was 30 minutes (bactericidal 3).

<Method for preparing chemical solution>
(A) component, (b) component, and (c) component are added to water in this order at the content ratio shown in the table so that the total is 100 g, and stirred and mixed until uniform at 25 ° C. After that, the pH at 25 ° C. was adjusted to 7 with hydrochloric acid.

<Method for preparing bacterial solution>
(1) A predetermined bacterium was inoculated with SMA (standard agar medium) and pre-cultured at 37 ° C. for 18 hours.
(2) After culture, the medium was centrifuged and the bacterial solution was collected, and the bacterial concentration of the bacterial solution was adjusted with physiological saline so that the optical density (OD) was 0.1.

<Method of evaluating bactericidal properties>
(1) 1 mL of a bacterial solution was added to 1 mL of a chemical solution and mixed to obtain a suspension.
(2) The suspension was left for a predetermined time.
(3) After standing, 0.2 mL of the suspension was quickly added to 1.8 mL of the LP solution to stop the effect of the antibacterial agent, and immediately 0.1 mL was smeared on SMA (standard agar medium).
Here, the above-mentioned LP solution is 30 g of “LP diluted solution“ DAIGO ”(manufactured by Nippon Pharmaceutical Co., Ltd.) (breakdown: polypeptone 1 g, lecithin 0.7 g, polysorbate 80 20 g, purified water 8.3 g) to 1 L with purified water. Diluted and sterilized at 120 ° C. for 20 minutes.
(4) Further, dilution by 10 times by mass with physiological saline was carried out stepwise and smeared on SMA in the same manner.
(5) After each smeared SMA was cultured at 37 ° C. for 24 hours, the number of grown colonies was counted, the number of viable bacteria per mL of the suspension was calculated, and the logarithm with 10 as the base was shown in the table.

The drugs described in Table 1, Table 2 and Table 3 are as follows.
Polylysine: ε-polylysine, food additive, weight average molecular weight 4,700 (manufactured by JNC Corporation)
・ Protamine: Protamine derived from salmon, protamine hydrochloride, processed food (manufactured by Maruha Nichiro Foods Co., Ltd.)
-Sucrose laurate: Ryoto sugar ester L-1695 (Mitsubishi Chemical Foods, Inc., monoester ratio content of about 80%, di-tri-polyester content of about 20%)
Sucrose myristic acid ester: Ryoto sugar ester M-1695 (manufactured by Mitsubishi Chemical Foods Co., Ltd., monoester ratio content about 80%, di-tri-polyester content about 20%)

  From the comparison of the examples and comparative examples in Table 1, Table 2 and Table 3, in the liquid germicide composition for hard surface of the present invention, the bactericidal properties of polylysine and protamine are synergistically improved by the sucrose fatty acid ester, It turns out that it is excellent in bactericidal property.

Formulation examples are described below.
Formulation Example 1: Kitchen disinfectant composition Polylysine 0.1% by mass
Protamine hydrochloride 0.1% by mass
Sucrose laurate 0.05% by mass
Sucrose myristic acid ester 0.05% by mass
Ethanol 2.5% by mass
Hydrochloric acid Amount adjusted to pH 7 Water balance Total 100% by mass

Claims (4)

The following (a) component, (b) component, (c) component:
(A) Component: Polylysine (b) Component: Protamine (c) Component: Liquid bactericidal composition for hard surface containing sucrose myristic acid ester and water.   The mass ratio of the total amount of the component (a) and the component (b) and the component (c) is [(a) + (b)] / (c), and is 0.001 or more and 25 or less. Liquid hard disinfectant composition for hard surfaces.   The liquid germicide composition for hard surfaces according to claim 1 or 2, wherein the pH at 25 ° C is 6 or more and 8 or less.   The hard surface sterilization method which applies the liquid germicide composition for hard surfaces in any one of Claims 1-3 to a hard surface.