JP6285934B2 - イムノアッセイの干渉の低減、及び安定化のためのシャペロン−シャペロン融合ポリペプチド - Google Patents

イムノアッセイの干渉の低減、及び安定化のためのシャペロン−シャペロン融合ポリペプチド Download PDF

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Publication number
JP6285934B2
JP6285934B2 JP2015530374A JP2015530374A JP6285934B2 JP 6285934 B2 JP6285934 B2 JP 6285934B2 JP 2015530374 A JP2015530374 A JP 2015530374A JP 2015530374 A JP2015530374 A JP 2015530374A JP 6285934 B2 JP6285934 B2 JP 6285934B2
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Prior art keywords
slyd
skp
fusion polypeptide
interference
slpa
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Japanese (ja)
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JP2015529211A (ja
JP2015529211A5 (enExample
Inventor
デューフェル，ハルトムート
リーデル，アレクサンダー
シャアーシュミット，ペーター
シュミット，ウルバン
ショルツ，クリスティアン
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y502/00—Cis-trans-isomerases (5.2)
    • C12Y502/01—Cis-trans-Isomerases (5.2.1)
    • C12Y502/01008—Peptidylprolyl isomerase (5.2.1.8), i.e. cyclophilin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/735—Fusion polypeptide containing domain for protein-protein interaction containing a domain for self-assembly, e.g. a viral coat protein (includes phage display)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
JP2015530374A 2012-09-06 2013-09-04 イムノアッセイの干渉の低減、及び安定化のためのシャペロン−シャペロン融合ポリペプチド Active JP6285934B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12006298.9 2012-09-06
EP12006298.9A EP2706115A1 (en) 2012-09-06 2012-09-06 Chaperone-chaperone fusion polypeptides for reduction of interference and stabilization of immunoassays
PCT/EP2013/068269 WO2014037389A1 (en) 2012-09-06 2013-09-04 Chaperone-chaperone fusion polypeptides for reduction of interference and stabilization of immunoassays

Publications (3)

Publication Number Publication Date
JP2015529211A JP2015529211A (ja) 2015-10-05
JP2015529211A5 JP2015529211A5 (enExample) 2016-09-15
JP6285934B2 true JP6285934B2 (ja) 2018-02-28

Family

ID=46963361

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2015530374A Active JP6285934B2 (ja) 2012-09-06 2013-09-04 イムノアッセイの干渉の低減、及び安定化のためのシャペロン−シャペロン融合ポリペプチド

Country Status (6)

Country Link
US (1) US10197559B2 (enExample)
EP (2) EP2706115A1 (enExample)
JP (1) JP6285934B2 (enExample)
CN (1) CN104583408B (enExample)
CA (1) CA2881411C (enExample)
WO (1) WO2014037389A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017153336A1 (en) * 2016-03-07 2017-09-14 F. Hoffmann-La Roche Ag Detection of anti-p53 antibodies
CN110996986B (zh) * 2017-07-27 2024-07-19 豪夫迈·罗氏有限公司 Hcv抗原的多表位融合蛋白及其用途
CA3076337A1 (en) 2017-09-19 2019-03-28 Massachusetts Institute Of Technology Compositions for chimeric antigen receptor t cell therapy and uses thereof
WO2021214248A1 (en) 2020-04-23 2021-10-28 F. Hoffmann-La Roche Ag Corona nucleocapsid antigen for use in antibody-immunoassays
EP4519285A1 (en) 2022-05-03 2025-03-12 F. Hoffmann-La Roche AG Hiv gp41 variants for immunodiagnostic assays
WO2025098623A1 (en) 2023-11-09 2025-05-15 Roche Diagnostics Gmbh Transglutaminase substrates for labeling
WO2026027625A1 (en) 2024-07-31 2026-02-05 Roche Diagnostics Gmbh Hepatitis delta virus polypeptides and detection

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100702373B1 (ko) 2001-06-22 2007-04-02 에프. 호프만-라 로슈 아게 레트로바이러스 표면 당단백질을 함유하는 가용성 복합체
JP4871960B2 (ja) * 2006-01-03 2012-02-08 エフ．ホフマン−ラ ロシュ アーゲー 優れたシャペロン活性およびフォールディング活性を有するキメラ融合タンパク質
ATE529436T1 (de) 2007-04-20 2011-11-15 Hoffmann La Roche Erkennung von primären infektionen mit krankheitserregern
CA2705716C (en) 2007-12-13 2013-03-19 F. Hoffmann-La Roche Ag Novel rubella e1 envelope protein variants and their use in the detection of anti-rubella antibodies
EP2127678A1 (en) * 2008-05-26 2009-12-02 Roche Diagnostics GmbH SlpA as a tool for recombinant protein and enzyme technology

Also Published As

Publication number Publication date
EP2893021B1 (en) 2016-08-24
EP2893021A1 (en) 2015-07-15
CN104583408A (zh) 2015-04-29
JP2015529211A (ja) 2015-10-05
HK1209784A1 (en) 2016-04-08
CN104583408B (zh) 2017-09-22
WO2014037389A1 (en) 2014-03-13
US20170261498A1 (en) 2017-09-14
EP2706115A1 (en) 2014-03-12
CA2881411A1 (en) 2014-03-13
US10197559B2 (en) 2019-02-05
CA2881411C (en) 2017-01-03

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