JP6285934B2 - イムノアッセイの干渉の低減、及び安定化のためのシャペロン−シャペロン融合ポリペプチド - Google Patents

イムノアッセイの干渉の低減、及び安定化のためのシャペロン−シャペロン融合ポリペプチド Download PDF

Info

Publication number
JP6285934B2
JP6285934B2 JP2015530374A JP2015530374A JP6285934B2 JP 6285934 B2 JP6285934 B2 JP 6285934B2 JP 2015530374 A JP2015530374 A JP 2015530374A JP 2015530374 A JP2015530374 A JP 2015530374A JP 6285934 B2 JP6285934 B2 JP 6285934B2
Authority
JP
Japan
Prior art keywords
slyd
skp
fusion polypeptide
interference
slpa
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2015530374A
Other languages
English (en)
Japanese (ja)
Other versions
JP2015529211A (ja
JP2015529211A5 (enExample
Inventor
デューフェル，ハルトムート
リーデル，アレクサンダー
シャアーシュミット，ペーター
シュミット，ウルバン
ショルツ，クリスティアン
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of JP2015529211A publication Critical patent/JP2015529211A/ja
Publication of JP2015529211A5 publication Critical patent/JP2015529211A5/ja
Application granted granted Critical
Publication of JP6285934B2 publication Critical patent/JP6285934B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y502/00—Cis-trans-isomerases (5.2)
    • C12Y502/01—Cis-trans-Isomerases (5.2.1)
    • C12Y502/01008—Peptidylprolyl isomerase (5.2.1.8), i.e. cyclophilin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/735—Fusion polypeptide containing domain for protein-protein interaction containing a domain for self-assembly, e.g. a viral coat protein (includes phage display)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
JP2015530374A 2012-09-06 2013-09-04 イムノアッセイの干渉の低減、及び安定化のためのシャペロン−シャペロン融合ポリペプチド Active JP6285934B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12006298.9 2012-09-06
EP12006298.9A EP2706115A1 (en) 2012-09-06 2012-09-06 Chaperone-chaperone fusion polypeptides for reduction of interference and stabilization of immunoassays
PCT/EP2013/068269 WO2014037389A1 (en) 2012-09-06 2013-09-04 Chaperone-chaperone fusion polypeptides for reduction of interference and stabilization of immunoassays

Publications (3)

Publication Number Publication Date
JP2015529211A JP2015529211A (ja) 2015-10-05
JP2015529211A5 JP2015529211A5 (enExample) 2016-09-15
JP6285934B2 true JP6285934B2 (ja) 2018-02-28

Family

ID=46963361

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2015530374A Active JP6285934B2 (ja) 2012-09-06 2013-09-04 イムノアッセイの干渉の低減、及び安定化のためのシャペロン−シャペロン融合ポリペプチド

Country Status (6)

Country Link
US (1) US10197559B2 (enExample)
EP (2) EP2706115A1 (enExample)
JP (1) JP6285934B2 (enExample)
CN (1) CN104583408B (enExample)
CA (1) CA2881411C (enExample)
WO (1) WO2014037389A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112018017336A2 (pt) * 2016-03-07 2018-12-26 Hoffmann La Roche método in vitro para detectar um anticorpo para p53 (anticorpo anti-p53) em uma amostra e polipeptídeo de fusão
JP7389740B2 (ja) * 2017-07-27 2023-11-30 エフ． ホフマン－ラ ロシュ アーゲー Ｈｃｖ抗原のマルチエピトープ融合タンパク質およびその使用
CA3076337A1 (en) 2017-09-19 2019-03-28 Massachusetts Institute Of Technology Compositions for chimeric antigen receptor t cell therapy and uses thereof
CN115843334B (zh) 2020-04-23 2026-01-13 豪夫迈·罗氏有限公司 用于抗体免疫测定的冠状核衣壳抗原
WO2023213758A1 (en) 2022-05-03 2023-11-09 F. Hoffmann-La Roche Ag Hiv gp41 variants for immunodiagnostic assays
CN122138968A (zh) 2023-11-09 2026-06-02 罗氏诊断公司 用于标记的转谷氨酰胺酶底物
WO2026027625A1 (en) 2024-07-31 2026-02-05 Roche Diagnostics Gmbh Hepatitis delta virus polypeptides and detection

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE522603T1 (de) 2001-06-22 2011-09-15 Hoffmann La Roche Löslicher komplex, der retrovirale oberflächen- glykoproteine und fkpa oder slyd enthält
JP4871960B2 (ja) * 2006-01-03 2012-02-08 エフ．ホフマン−ラ ロシュ アーゲー 優れたシャペロン活性およびフォールディング活性を有するキメラ融合タンパク質
ATE516298T1 (de) 2007-04-20 2011-07-15 Hoffmann La Roche Erkennung von primären infektionen mit krankheitserregern
CN101939330B (zh) 2007-12-13 2014-06-04 霍夫曼-拉罗奇有限公司 新型风疹e1包膜蛋白变体及其在检测抗风疹抗体中的用途
EP2127678A1 (en) * 2008-05-26 2009-12-02 Roche Diagnostics GmbH SlpA as a tool for recombinant protein and enzyme technology

Also Published As

Publication number Publication date
JP2015529211A (ja) 2015-10-05
CN104583408A (zh) 2015-04-29
US10197559B2 (en) 2019-02-05
EP2893021A1 (en) 2015-07-15
EP2706115A1 (en) 2014-03-12
US20170261498A1 (en) 2017-09-14
WO2014037389A1 (en) 2014-03-13
CA2881411A1 (en) 2014-03-13
CA2881411C (en) 2017-01-03
CN104583408B (zh) 2017-09-22
HK1209784A1 (en) 2016-04-08
EP2893021B1 (en) 2016-08-24

Similar Documents

Publication Publication Date Title
CA2626423C (en) Detection of primary infections with pathogens
JP2015529211A (ja) イムノアッセイの干渉の低減、及び安定化のためのシャペロン−シャペロン融合ポリペプチド
CN101591665B (zh) 作为重组蛋白和酶技术的工具的SlpA
CA2856187C (en) Soluble immunoreactive treponema pallidum tpn47 antigens
US11567079B2 (en) Soluble and immunoreactive variants of HTLV capsid antigen P24
CA2856627C (en) Vibrio cholerae lipoprotein 15 (lp15) variants as anti-interference additive in tpn17-based immunoassays for detection of anti-treponema antibodies
HK1209784B (en) Chaperone-chaperone fusion polypeptides for reduction of interference and stabilization of immunoassays
HK1229824B (en) Soluble and immunoreactive variants of htlv capsid antigen p24
HK1201919B (en) Vibrio cholerae lipoprotein 15 (lp15) variants as anti-interference additive in tpn17-based immunoassays for detection of anti-treponema antibodies
HK1229824A1 (en) Soluble and immunoreactive variants of htlv capsid antigen p24
HK1138620B (en) Slpa as a tool for recombinant protein and enzyme technology

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20160727

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20160727

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20170523

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20170601

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20170802

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20180104

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20180202

R150 Certificate of patent or registration of utility model

Ref document number: 6285934

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250