CA2881411C - Chaperone-chaperone fusion polypeptides for reduction of interference and stabilization of immunoassays - Google Patents

Chaperone-chaperone fusion polypeptides for reduction of interference and stabilization of immunoassays Download PDF

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Publication number
CA2881411C
CA2881411C CA2881411A CA2881411A CA2881411C CA 2881411 C CA2881411 C CA 2881411C CA 2881411 A CA2881411 A CA 2881411A CA 2881411 A CA2881411 A CA 2881411A CA 2881411 C CA2881411 C CA 2881411C
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CA
Canada
Prior art keywords
slyd
skp
fusion polypeptide
interference
fusion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2881411A
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English (en)
French (fr)
Other versions
CA2881411A1 (en
Inventor
Hartmut Duefel
Alexander Riedel
Peter Schaarschmidt
Urban Schmitt
Christian Scholz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of CA2881411A1 publication Critical patent/CA2881411A1/en
Application granted granted Critical
Publication of CA2881411C publication Critical patent/CA2881411C/en
Active legal-status Critical Current
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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y502/00—Cis-trans-isomerases (5.2)
    • C12Y502/01—Cis-trans-Isomerases (5.2.1)
    • C12Y502/01008—Peptidylprolyl isomerase (5.2.1.8), i.e. cyclophilin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/735—Fusion polypeptide containing domain for protein-protein interaction containing a domain for self-assembly, e.g. a viral coat protein (includes phage display)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
CA2881411A 2012-09-06 2013-09-04 Chaperone-chaperone fusion polypeptides for reduction of interference and stabilization of immunoassays Active CA2881411C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12006298.9 2012-09-06
EP12006298.9A EP2706115A1 (en) 2012-09-06 2012-09-06 Chaperone-chaperone fusion polypeptides for reduction of interference and stabilization of immunoassays
PCT/EP2013/068269 WO2014037389A1 (en) 2012-09-06 2013-09-04 Chaperone-chaperone fusion polypeptides for reduction of interference and stabilization of immunoassays

Publications (2)

Publication Number Publication Date
CA2881411A1 CA2881411A1 (en) 2014-03-13
CA2881411C true CA2881411C (en) 2017-01-03

Family

ID=46963361

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2881411A Active CA2881411C (en) 2012-09-06 2013-09-04 Chaperone-chaperone fusion polypeptides for reduction of interference and stabilization of immunoassays

Country Status (6)

Country Link
US (1) US10197559B2 (enExample)
EP (2) EP2706115A1 (enExample)
JP (1) JP6285934B2 (enExample)
CN (1) CN104583408B (enExample)
CA (1) CA2881411C (enExample)
WO (1) WO2014037389A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112018017336A2 (pt) * 2016-03-07 2018-12-26 Hoffmann La Roche método in vitro para detectar um anticorpo para p53 (anticorpo anti-p53) em uma amostra e polipeptídeo de fusão
JP7389740B2 (ja) * 2017-07-27 2023-11-30 エフ． ホフマン－ラ ロシュ アーゲー Ｈｃｖ抗原のマルチエピトープ融合タンパク質およびその使用
CA3076337A1 (en) 2017-09-19 2019-03-28 Massachusetts Institute Of Technology Compositions for chimeric antigen receptor t cell therapy and uses thereof
CN115843334B (zh) 2020-04-23 2026-01-13 豪夫迈·罗氏有限公司 用于抗体免疫测定的冠状核衣壳抗原
WO2023213758A1 (en) 2022-05-03 2023-11-09 F. Hoffmann-La Roche Ag Hiv gp41 variants for immunodiagnostic assays
CN122138968A (zh) 2023-11-09 2026-06-02 罗氏诊断公司 用于标记的转谷氨酰胺酶底物
WO2026027625A1 (en) 2024-07-31 2026-02-05 Roche Diagnostics Gmbh Hepatitis delta virus polypeptides and detection

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE522603T1 (de) 2001-06-22 2011-09-15 Hoffmann La Roche Löslicher komplex, der retrovirale oberflächen- glykoproteine und fkpa oder slyd enthält
JP4871960B2 (ja) * 2006-01-03 2012-02-08 エフ．ホフマン−ラ ロシュ アーゲー 優れたシャペロン活性およびフォールディング活性を有するキメラ融合タンパク質
ATE516298T1 (de) 2007-04-20 2011-07-15 Hoffmann La Roche Erkennung von primären infektionen mit krankheitserregern
CN101939330B (zh) 2007-12-13 2014-06-04 霍夫曼-拉罗奇有限公司 新型风疹e1包膜蛋白变体及其在检测抗风疹抗体中的用途
EP2127678A1 (en) * 2008-05-26 2009-12-02 Roche Diagnostics GmbH SlpA as a tool for recombinant protein and enzyme technology

Also Published As

Publication number Publication date
JP2015529211A (ja) 2015-10-05
CN104583408A (zh) 2015-04-29
US10197559B2 (en) 2019-02-05
JP6285934B2 (ja) 2018-02-28
EP2893021A1 (en) 2015-07-15
EP2706115A1 (en) 2014-03-12
US20170261498A1 (en) 2017-09-14
WO2014037389A1 (en) 2014-03-13
CA2881411A1 (en) 2014-03-13
CN104583408B (zh) 2017-09-22
HK1209784A1 (en) 2016-04-08
EP2893021B1 (en) 2016-08-24

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