JP6232074B2 - 改変され、そして改善された細胞および生物を生成するための組成物および方法 - Google Patents

改変され、そして改善された細胞および生物を生成するための組成物および方法 Download PDF

Info

Publication number
JP6232074B2
JP6232074B2 JP2015549712A JP2015549712A JP6232074B2 JP 6232074 B2 JP6232074 B2 JP 6232074B2 JP 2015549712 A JP2015549712 A JP 2015549712A JP 2015549712 A JP2015549712 A JP 2015549712A JP 6232074 B2 JP6232074 B2 JP 6232074B2
Authority
JP
Japan
Prior art keywords
orf
primer
randomized
sequence
pcr
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2015549712A
Other languages
English (en)
Japanese (ja)
Other versions
JP2016501039A (ja
Inventor
ヘルゲ・ジーラー
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority claimed from US14/134,619 external-priority patent/US9200291B2/en
Publication of JP2016501039A publication Critical patent/JP2016501039A/ja
Application granted granted Critical
Publication of JP6232074B2 publication Critical patent/JP6232074B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2015549712A 2012-12-19 2013-12-19 改変され、そして改善された細胞および生物を生成するための組成物および方法 Active JP6232074B2 (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201261739671P 2012-12-19 2012-12-19
US61/739,671 2012-12-19
US201361761175P 2013-02-05 2013-02-05
US61/761,175 2013-02-05
US14/134,619 2013-12-19
US14/134,619 US9200291B2 (en) 2012-12-19 2013-12-19 Compositions and methods for creating altered and improved cells and organisms
PCT/US2013/076526 WO2014100400A1 (en) 2012-12-19 2013-12-19 Compositions and methods for creating altered and improved cells and organisms

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2016185318A Division JP2017029159A (ja) 2012-12-19 2016-09-23 改変され、そして改善された細胞および生物を生成するための組成物および方法

Publications (2)

Publication Number Publication Date
JP2016501039A JP2016501039A (ja) 2016-01-18
JP6232074B2 true JP6232074B2 (ja) 2017-11-15

Family

ID=50979211

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2015549712A Active JP6232074B2 (ja) 2012-12-19 2013-12-19 改変され、そして改善された細胞および生物を生成するための組成物および方法
JP2016185318A Pending JP2017029159A (ja) 2012-12-19 2016-09-23 改変され、そして改善された細胞および生物を生成するための組成物および方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2016185318A Pending JP2017029159A (ja) 2012-12-19 2016-09-23 改変され、そして改善された細胞および生物を生成するための組成物および方法

Country Status (6)

Country Link
EP (1) EP2935582B1 (enExample)
JP (2) JP6232074B2 (enExample)
AU (1) AU2013361289B2 (enExample)
DK (1) DK2935582T3 (enExample)
IL (1) IL239529B (enExample)
WO (1) WO2014100400A1 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2802663B1 (en) * 2012-01-12 2018-05-02 Archer Daniels Midland Co. Genes conferring tolerance to ethanol and high temperatures for yeasts
US10556935B2 (en) 2014-07-31 2020-02-11 Primordial Genetics Inc. Compositions for improving cells and organisms
CN114269896B (zh) * 2019-06-24 2025-02-07 丹尼斯科美国公司 酵母中用于增加醇和赖氨酸生产的cdc42效应因子破坏

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU1350801A (en) * 1999-10-27 2001-05-08 Frances Arnold Production of functional hybrid genes and proteins
US20040204953A1 (en) * 2002-07-17 2004-10-14 Lincoln Muir Subscription based systems, methods and components for providing genomic and proteomic products and services
DE602007008067D1 (de) * 2006-02-14 2010-09-09 Firmenich & Cie Verfahren zur herstellung von terpenen und mep-transformierte mikroorganismen dafür
US8268564B2 (en) * 2007-09-26 2012-09-18 President And Fellows Of Harvard College Methods and applications for stitched DNA barcodes
US9315806B2 (en) * 2012-06-29 2016-04-19 Massachusetts Institute Of Technology Massively parallel combinatorial genetics

Also Published As

Publication number Publication date
WO2014100400A1 (en) 2014-06-26
JP2016501039A (ja) 2016-01-18
DK2935582T3 (da) 2020-03-02
EP2935582B1 (en) 2019-12-04
AU2013361289A1 (en) 2015-08-06
JP2017029159A (ja) 2017-02-09
AU2013361289B2 (en) 2016-05-19
IL239529A0 (en) 2015-08-31
EP2935582A1 (en) 2015-10-28
IL239529B (en) 2019-11-28

Similar Documents

Publication Publication Date Title
US9200291B2 (en) Compositions and methods for creating altered and improved cells and organisms
AU2006347573B2 (en) Synthetic genomes
AU2015217208B2 (en) CRISPR enabled multiplexed genome engineering
CA3223527A1 (en) Novel crispr enzymes and systems
JP2018532419A (ja) ＣＲＩＳＰＲ−Ｃａｓ ｓｇＲＮＡライブラリー
KR20010083053A (ko) 반복적 서열 재조합에 의한 전체 세포 및 유기체의 진화
KR102345898B1 (ko) 글루코오스 투과 효소 라이브러리를 생성하는 방법 및 이의 용도
WO2022261122A1 (en) Crispr-transposon systems for dna modification
KR20190025615A (ko) 박테리아 헤모글로빈 라이브러리를 생성하는 방법 및 이의 용도
CN116179513B (zh) 一种Cpf1蛋白及其在基因编辑中的应用
KR20190098213A (ko) 유전자 조작 및 균주 정제를 위한 자동화 단계를 사용하여 균류 생산 균주를 제조하는 방법
US20230257799A1 (en) Methods of identifying and characterizing gene editing variations in nucleic acids
JP6232074B2 (ja) 改変され、そして改善された細胞および生物を生成するための組成物および方法
Burian et al. High-throughput retrieval of target sequences from complex clone libraries using CRISPRi
CN119156446A (zh) Rna靶向基因编辑工具的开发
EP3874037A1 (en) Multiplexed deterministic assembly of dna libraries
US20190002873A1 (en) Methods and compositions for creating altered and improved cells and organisms
Class et al. Patent application title: COMPOSITIONS AND METHODS FOR CREATING ALTERED AND IMPROVED CELLS AND ORGANISMS Inventors: Helge Zieler (Encinitas, CA, US) Helge Zieler
CN117327683B (zh) 一种在DNA的gC基序上实现高效C/G到T/A编辑的高保真LnCBE系统
Thomy et al. Genomic analysis of Ostreococcus tauri-infecting viruses reveals a hypervariable region associated with host–virus interactions
Costa et al. An extracellular, Ca 2+-activated nuclease (EcnA) mediates transformation in a naturally competent archaeon.
CN117795085A (zh) 用于dna修饰的crispr-转座子系统
CN121152876A (zh) 高通量筛选和测序方法
Joshi Molecular Biology and Biotechnology
Siva et al. TEXTBOOK OF BIOTECHNOLOGY

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20151020

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20151020

A871 Explanation of circumstances concerning accelerated examination

Free format text: JAPANESE INTERMEDIATE CODE: A871

Effective date: 20151020

A975 Report on accelerated examination

Free format text: JAPANESE INTERMEDIATE CODE: A971005

Effective date: 20151117

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20151222

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20160322

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20160523

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20160923

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20161108

A912 Re-examination (zenchi) completed and case transferred to appeal board

Free format text: JAPANESE INTERMEDIATE CODE: A912

Effective date: 20161228

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20171020

R150 Certificate of patent or registration of utility model

Ref document number: 6232074

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250