JP6215929B2 - 生物学的及び/または化学的検定を実行するための方法及び装置 - Google Patents
生物学的及び/または化学的検定を実行するための方法及び装置 Download PDFInfo
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- JP6215929B2 JP6215929B2 JP2015520983A JP2015520983A JP6215929B2 JP 6215929 B2 JP6215929 B2 JP 6215929B2 JP 2015520983 A JP2015520983 A JP 2015520983A JP 2015520983 A JP2015520983 A JP 2015520983A JP 6215929 B2 JP6215929 B2 JP 6215929B2
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
- B01L2200/0668—Trapping microscopic beads
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/08—Regulating or influencing the flow resistance
- B01L2400/084—Passive control of flow resistance
- B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/2575—Volumetric liquid transfer
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- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Pathology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- General Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Food Science & Technology (AREA)
- Dispersion Chemistry (AREA)
- Fluid Mechanics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Physical Or Chemical Processes And Apparatus (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Description
a)少なくとも一つの入口及び少なくとも一つの出口を有する少なくとも一つのマイクロチャネルを備える検定装置を提供するステップであって、前記検定装置はさらに、マイクロチャネル内に導入されるマイクロ粒子の出口への動きを制限するように設計された制限手段を備え、一方で流体を出口に向かって制限手段を通って流すステップ。
b)入口を介してマイクロチャネル内に複数のマイクロ粒子を導入するステップであって、各マイクロ粒子がマイクロチャネルの断面に対して隣接する二つのマイクロ粒子の重なりを防ぐ形状を有するステップ。
c)制限手段を用いて、前記マイクロ粒子のマイクロチャネル内における出口への動きを制限するステップ。
d)マイクロチャネルを通して流体試料を流すステップ。
e)各マイクロ粒子について生物学的及び/または化学的読出しを実行するステップ。この方法は更に以下のステップを備える:
f)マイクロチャネル内でマイクロ粒子を動かし、一方でマイクロ粒子の出口への動きは依然として前記制限手段によって制限されるステップ。
g)ステップ(d)及び(e)を連続的に繰り返すステップ。
12 横断穴
14 L字サイン
16 上面
18 下面
20 マイクロチャネル
22 マイクロ粒子
28 入口
30 入口ウェル
32 出口ウェル
34 制限手段
38、40 マイクロポンプ
101、102、103、104 マイクロ粒子
Claims (8)
- 少なくとも以下の連続ステップを備える化学的及び/または生物学的検定を実行するための方法であって、ステップ(f)及び(g)が0.05Hzから5Hzの範囲内の所定の周波数で周期的に繰り返される、方法:
(a)少なくとも一つの入口(28)及び少なくとも一つの出口(30)を有する少なくとも一つのマイクロチャネル(20)を備える検定装置(19)を提供するステップであって、前記検定装置が、前記マイクロチャネル(20)内に導入されたマイクロ粒子(10)の前記出口(30)への動きを制限するように設計され、一方で流体を、制限手段(34)を通って前記出口(30)へと流す制限手段(34)をさらに備えるステップ、
(b)前記入口(28)を介して前記マイクロチャネル(20)内に複数のマイクロ粒子(10)を導入するステップであって、各マイクロ粒子(10)が、前記マイクロチャネル(20)の断面に対して隣接する二つのマイクロ粒子(10)の重なりを防ぐ形状を有し、前記マイクロ粒子は前記マイクロチャネルに沿って測定された寸法dを有するステップ、
(c)制限手段(34)を用いて、前記マイクロ粒子(10)の前記マイクロチャネル(20)内における前記出口への動きを制限するステップ、
(d)前記マイクロチャネル(20)を通って流体試料を流すステップ、
(e)各マイクロ粒子(10)について生物学的及び/または化学的読出しを実行するステップであって、前記読出しは前記生物学的及び/または化学的検定と関連して発された信号を検出することを含むステップ、
(f)前記マイクロチャネル(20)に沿って前記入口(28)に向かって後方へと、その後、前記マイクロチャネル(20)に沿って前記出口(30)に向かって前方へと前記マイクロ粒子(101、102、103、104)を動かす一方で、前記制限手段が前記マイクロ粒子の前記出口(30)に向かう方向の動きを制限し、前記マイクロ粒子の後方及び前方への動きが前記マイクロチャネルに沿って前記マイクロ粒子の互いに対して空間的かつランダムな再配置を起こすステップであって、前記マイクロ粒子は前記寸法dの少なくとも5倍を表す距離で前記マイクロチャネルの入口に向かって動かされるステップ、及び
(g)ステップ(d)及び(e)を連続的に繰り返すステップ。 - 前記所定の周波数が0.05Hzから1Hzの範囲内である、請求項1に記載の方法。
- 前記ステップ(f)が第一の所定の期間実行される、請求項1に記載の方法。
- 前記第一の所定の期間が1秒から5秒の範囲内である、請求項3に記載の方法。
- 前記ステップ(g)が第二の所定の期間実行される、請求項1に記載の方法。
- 前記第二の所定の期間が0.5秒から5秒の範囲内である、請求項5に記載の方法。
- 前記ステップ(f)の間に、前記マイクロ粒子(10)が、前記マイクロチャネル(20)の前記入口(28)と前記出口(30)との間の流体試料の負差圧(ΔP)を印加することで後方に動かされ、前記マイクロチャネル(20)の前記入口(28)と前記出口(30)との間の流体試料の正差圧(ΔP)を印加することで前方に動かされる、請求項1に記載の方法。
- 前記ステップ(f)と前記ステップ(g)との間に実施される、前記マイクロ粒子(10)の動きが前記制限手段(34)によって制限されるまで、前記マイクロ粒子(10)を前記出口(30)へと動かすことを含むステップ(f’)をさらに備える、請求項1に記載の方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP12175954.2A EP2685262A1 (en) | 2012-07-11 | 2012-07-11 | Method and device for performing biological and/or chemical assays |
EP12175954.2 | 2012-07-11 | ||
PCT/EP2013/064635 WO2014009446A1 (en) | 2012-07-11 | 2013-07-10 | A method and device for performing biological and/or chemical assays |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2015522170A JP2015522170A (ja) | 2015-08-03 |
JP6215929B2 true JP6215929B2 (ja) | 2017-10-18 |
Family
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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JP2015520983A Expired - Fee Related JP6215929B2 (ja) | 2012-07-11 | 2013-07-10 | 生物学的及び/または化学的検定を実行するための方法及び装置 |
Country Status (13)
Country | Link |
---|---|
US (1) | US9625455B2 (ja) |
EP (2) | EP2685262A1 (ja) |
JP (1) | JP6215929B2 (ja) |
KR (1) | KR20150036514A (ja) |
CN (1) | CN104471396B (ja) |
AU (1) | AU2013288755B2 (ja) |
BR (1) | BR112015000294A2 (ja) |
CA (1) | CA2876279A1 (ja) |
ES (1) | ES2774228T3 (ja) |
IN (1) | IN2014DN10718A (ja) |
RU (1) | RU2014150439A (ja) |
WO (1) | WO2014009446A1 (ja) |
ZA (1) | ZA201409260B (ja) |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE60021070T2 (de) | 1999-04-16 | 2006-04-20 | Tibotec Bvba | Kodierung von mikroträgern |
JP4533685B2 (ja) * | 2004-07-01 | 2010-09-01 | セイコーインスツル株式会社 | マイクロ流体装置 |
JP2007155484A (ja) * | 2005-12-05 | 2007-06-21 | Rohm Co Ltd | マイクロチップ |
US8124015B2 (en) * | 2006-02-03 | 2012-02-28 | Institute For Systems Biology | Multiplexed, microfluidic molecular assay device and assay method |
JP5560039B2 (ja) * | 2006-10-05 | 2014-07-23 | マサチューセッツ インスティテュート オブ テクノロジー | ハイスループット分析のための多機能のコード化された粒子 |
KR100878229B1 (ko) * | 2007-11-22 | 2009-01-12 | 주식회사 디지탈바이오테크놀러지 | 유체분석용 칩 |
KR101656846B1 (ko) * | 2008-12-23 | 2016-09-12 | 미카티스 엔브이 | 생물학적 분석들을 수행하기 위한 분석 장치 및 방법 |
JP5663985B2 (ja) * | 2009-12-16 | 2015-02-04 | ソニー株式会社 | マイクロビーズ検査用のセル及びマイクロビーズの解析方法 |
KR100961874B1 (ko) * | 2010-04-05 | 2010-06-09 | 주식회사 나노엔텍 | 외부동력 없이 유체가 이동하는 유체분석용 칩 |
-
2012
- 2012-07-11 EP EP12175954.2A patent/EP2685262A1/en not_active Withdrawn
-
2013
- 2013-07-10 AU AU2013288755A patent/AU2013288755B2/en not_active Ceased
- 2013-07-10 JP JP2015520983A patent/JP6215929B2/ja not_active Expired - Fee Related
- 2013-07-10 ES ES13737588T patent/ES2774228T3/es active Active
- 2013-07-10 EP EP13737588.7A patent/EP2872890B1/en active Active
- 2013-07-10 RU RU2014150439A patent/RU2014150439A/ru not_active Application Discontinuation
- 2013-07-10 CA CA2876279A patent/CA2876279A1/en not_active Abandoned
- 2013-07-10 WO PCT/EP2013/064635 patent/WO2014009446A1/en active Application Filing
- 2013-07-10 IN IN10718DEN2014 patent/IN2014DN10718A/en unknown
- 2013-07-10 BR BR112015000294A patent/BR112015000294A2/pt not_active Application Discontinuation
- 2013-07-10 US US14/413,314 patent/US9625455B2/en not_active Expired - Fee Related
- 2013-07-10 CN CN201380036815.9A patent/CN104471396B/zh not_active Expired - Fee Related
- 2013-07-10 KR KR1020157003541A patent/KR20150036514A/ko not_active Application Discontinuation
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2014
- 2014-12-15 ZA ZA2014/09260A patent/ZA201409260B/en unknown
Also Published As
Publication number | Publication date |
---|---|
JP2015522170A (ja) | 2015-08-03 |
ES2774228T3 (es) | 2020-07-17 |
CN104471396A (zh) | 2015-03-25 |
IN2014DN10718A (ja) | 2015-09-04 |
CA2876279A1 (en) | 2014-01-16 |
EP2685262A1 (en) | 2014-01-15 |
RU2014150439A (ru) | 2016-07-10 |
AU2013288755A1 (en) | 2015-01-22 |
EP2872890A1 (en) | 2015-05-20 |
ZA201409260B (en) | 2015-12-23 |
EP2872890B1 (en) | 2019-12-04 |
AU2013288755B2 (en) | 2018-05-10 |
CN104471396B (zh) | 2018-05-04 |
KR20150036514A (ko) | 2015-04-07 |
US9625455B2 (en) | 2017-04-18 |
BR112015000294A2 (pt) | 2017-06-27 |
WO2014009446A1 (en) | 2014-01-16 |
US20150141281A1 (en) | 2015-05-21 |
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