JP6185743B2 - Hair growth inhibitor - Google Patents

Description

  The present invention relates to a hair growth inhibitor.

  Biologically, hair or body hair protects important organs such as the head, chest, and limbs. However, in recent years, there is an increasing tendency that hair on limbs and the like is preferably not in terms of aesthetic appearance.

  As a method for removing body hair, a mechanical removal method using a tweezer, a shaver, a hair removal device, or the like, and a removal method by a chemical action using a hair removal agent or a hair removal agent are known. However, these known hair removal methods may involve physical or chemical irritation to the skin. Moreover, in the said known hair removal method, since the hair growth of the hair reproduced | regenerated after removal cannot be suppressed continuously, after a fixed period passes, the hair removal process must be performed again. Therefore, there is a demand for a hair growth inhibitor that can reduce the number of treatments and reduce labor by continuously and effectively suppressing the growth of body hair.

  Conventionally, the search of the plant-derived component which has the effect | action which suppresses hair growth is performed. As for the plantaceae, the hair growth inhibitor (Patent Document 1) consisting of Dioh (Rheum palmatum L., Rheum tanguticum Maximowicz, Rheum officinale Baillon, Rheum coreanum Nakai or their hybrids) or its extract, and tartary buckwheat (Fagopyrum tataricum) A hair growth inhibitor (Patent Document 2) containing an extract of L. Gaertn) as an active ingredient is known.

  Mukagotorano is a plant belonging to the genus Ibukitrano. Recently, it has been reported that mugagotranoo has an anti-inflammatory effect (Non-patent Document 1), but no other medicinal effects are known.

JP 2003-089626 A JP 2011-190214 A

J Sci Food Agric, 2012, Nov 5., doi: 10.1002 / jsfa.5795

  The present invention relates to providing a hair growth inhibitor that effectively and continuously suppresses hair growth.

  As a result of searching for a substance that suppresses hair growth, the present inventors have found that a centropod plant, Mukagotoranoo, or an extract thereof, which has been hardly known to have some medicinal effects, has an effect of suppressing hair growth. It has been found effective for hair removal or hair removal.

That is, this invention provides the hair growth inhibitor which uses mukagotoranoo or its extract as an active ingredient.
The present invention also provides a hair removal or depilation agent comprising musgotranoo or its extract as an active ingredient.
Moreover, this invention provides the skin external preparation which uses mukagotoranoo or its extract as an active ingredient.

  According to the present invention, it is possible to provide a hair removal agent, a hair removal agent, a skin external preparation and the like that are derived from plants and are safe for living bodies and have a high hair growth inhibitory effect.

The effect of musgotranoo extract on hair growth.

  In the present specification, “hair growth inhibition” means an action of suppressing the growth of hair or hair follicles or an action of reducing hair diameter.

  In this specification, examples of the “hair” whose growth is suppressed include head hair, beard, and body hair other than head hair such as limbs and trunk hair, but hair other than beard and head hair is preferred.

  As used herein, “non-therapeutic” does not include medical practice, that is, does not include methods for surgery, treatment or diagnosis of humans, more specifically a doctor, or a health care professional or doctor's instructions. It is a concept that does not include a method for the recipient to perform surgery, treatment or diagnosis on humans.

  In the present specification, “Mukagotoranoo” refers to Polygonum viviparum L. belonging to the genus Ibukitrano. In the present invention, mugagotrano can be any part thereof, for example, whole grass, leaves (leaf blade, petiole, etc.), fruits (mature, immature, etc.), seeds, flowers (petals, ovary, etc.), stem, above-ground part , Rhizomes, roots, tuberous roots, or combinations thereof may be used. Of these, the preferred site is the rhizome. In the present invention, the plant may be used as it is or after being dried, or may be used by cutting, crushing, crushing, and exploiting them.

  The plant extract can be obtained by subjecting the plant part described above to the extraction step as it is or after drying, or further cutting, crushing, and pulverizing. As the plant extract, commercially available products may be used, or various solvent extracts obtained by conventional methods, or diluted solutions, concentrated solutions, dried powders, or pastes. It may be.

  As the solvent for extraction, either a polar solvent or a nonpolar solvent can be used. Specific examples of the solvent include, for example, water; monovalent, divalent or polyhydric alcohols; ketones such as acetone and methyl ethyl ketone; esters such as methyl acetate and ethyl acetate; Cyclic ethers; polyethers such as polyethylene glycol; saturated or unsaturated hydrocarbons; aromatic hydrocarbons such as benzene and toluene; halogenated hydrocarbons such as dichloromethane, chloroform, dichloroethane, and carbon tetrachloride; Pyridines; dimethyl sulfoxide; acetonitrile; carbon dioxide, supercritical carbon dioxide; fats and oils, waxes, other oils; and mixtures thereof. Preferable examples include water, alcohols and aqueous solutions thereof in terms of pharmacological activity and versatility.

  Examples of the alcohols include monohydric alcohols such as methanol, ethanol, propanol, butanol, amyl alcohol, hexanol, heptanol, octanol; ethylene glycol, propylene glycol, 1,3-butylene glycol, 1,4-butanediol. 2, dihydric alcohols such as 1,5-pentanediol and 1,6-hexanediol; trihydric or higher alcohols such as glycerin. Among these, monohydric alcohols and dihydric alcohols are preferable from the viewpoint of versatility. Preferably, the alcohol may be an alcohol having 1 to 10 carbon atoms, more preferably an alcohol having 1 to 4 carbon atoms.

  Preferable examples of the alcohols include methanol, ethanol, 1,3-butylene glycol, n-propanol, isopropanol, n-butanol, isobutanol, sec-butanol, t-butanol and the like.

  The concentration of alcohol in the aqueous solution of the alcohol is preferably 20% by volume or more, more preferably 30% by volume or more, and preferably 99.9% by volume or less, more preferably 99.5% by volume or less, Preferably it is 95 volume% or less. Alternatively, the concentration of the alcohol in the aqueous solution of the alcohol is preferably 20% to 99.9% by volume, more preferably 20% to 99.5% by volume, and even more preferably 30% to 95% by volume. It is as follows.

  Of the alcohols, ethanol is more preferable from the viewpoint of versatility. Therefore, more suitable solvents for preparing the plant extract include water, ethanol and aqueous ethanol.

  The ethanol concentration of the aqueous ethanol solution is preferably 20% by volume or more, more preferably 30% by volume or more, and preferably 99.9% by volume or less, more preferably 99.5% by volume or less, and still more preferably 95% by volume. % Or less. Alternatively, the ethanol concentration of the aqueous ethanol solution is preferably 20% to 99.9% by volume, more preferably 20% to 99.5% by volume, and still more preferably 30% to 95% by volume.

  As a usage-amount of the solvent in extraction, 1-100 mL is preferable with respect to 1 g of plants (dry mass conversion). The extraction conditions are not particularly limited as long as sufficient extraction can be performed. For example, the extraction time is preferably 1 minute or longer, more preferably 10 minutes or longer, on the other hand, preferably 2 months or shorter, more preferably 5 weeks or shorter. The extraction temperature is preferably 0 ° C. or higher, more preferably 5 ° C. or higher, and the solvent boiling point or lower is preferable. Usually, extraction is performed for a long time at a low temperature and for a short time at a high temperature. Examples of extraction conditions include 15 to 40 ° C. for 3 days to 5 weeks, but are not limited thereto, and can be appropriately selected by those skilled in the art.

  Extraction means for obtaining the plant extract is, for example, usual means such as solid-liquid extraction, liquid-liquid extraction, immersion, decoction, leaching, reflux extraction, Soxhlet extraction, ultrasonic extraction, microwave extraction, stirring, etc. Can be used. These extraction means may be combined. For example, dipping or solid-liquid extraction may be combined with liquid-liquid extraction, or when the extraction time is shortened, solid-liquid extraction with stirring may be performed. Furthermore, in order to prevent the oxidation of the extract, a means for extracting under a so-called non-oxidizing atmosphere while removing dissolved oxygen by bubbling degassing or inert gas such as nitrogen gas may be used in combination.

  The extract obtained as described above can be used as it is, but it can also be diluted, concentrated or freeze-dried, and / or prepared in a liquid, powder or paste form. Alternatively, treatment using activated carbon, adsorption resin, ion exchange resin, activated clay, silica gel or the like, which is generally used for production of plant extracts, can also be performed.

  In the present invention, any one of Mucagotorano and its extract may be used alone, or may be used in a combination of two or more selected from the group consisting of the above-mentioned Mukatagono and its extract. The extract may be any one of the various solvent extracts described above or a combination of two or more of the above solvent extracts, but preferably includes an aqueous ethanol extract, and more preferably an aqueous ethanol extract. The ethanol aqueous solution extract preferably has an ethanol concentration of 20% by volume or more, more preferably 30% by volume or more, and preferably 99.9% by volume or less, more preferably 99.5% by volume or less, and still more preferably 95% by volume. It may be an extract of a volume% or less aqueous ethanol solution. Alternatively, the ethanol aqueous solution extract preferably has an ethanol concentration of 20% by volume to 99.9% by volume, more preferably 20% by volume to 99.5% by volume, and even more preferably 30% by volume to 95% by volume. An extract of an aqueous ethanol solution.

  The above-mentioned mugagotoranoo or an extract thereof can suppress hair growth in humans or non-human animals, or tissues, organs and cells derived therefrom, and thereby exerts an effect on hair removal, hair loss and the like. be able to. For example, the above-mentioned muggotrano or its extract can remove hair from the skin by its hair growth inhibitory action. Alternatively, the above-mentioned mugagotoranoo or its extract reduces hair number and thickness by reducing hair growth, facilitates hair removal or hair removal treatment, or delays hair growth after treatment to remove hair. And the duration of hair removal treatment can be increased, and the frequency of necessary hair removal and hair removal treatment can be reduced.

Therefore, in one aspect, the present invention provides a hair growth inhibitor containing musgotranoo or an extract thereof as an active ingredient. In addition, the present invention provides a hair removal agent or a hair removal agent comprising musgotranoo or an extract thereof as an active ingredient.
In another aspect, the present invention provides the use of a hair growth inhibitor, or a mugagotrano or extract thereof for the manufacture of a hair removal or hair removal agent.
In one embodiment, the agent may consist essentially of mugagotrano or an extract thereof.

In yet another aspect, the present invention provides the use of mugagotrano or an extract thereof for hair growth inhibition or for hair removal or hair removal.
In yet another aspect, the present invention provides a mugagotrano or extract thereof for use in hair growth inhibition or hair removal or hair removal.
The use may be a therapeutic use but may also be a non-therapeutic use. Non-therapeutic uses include hair removal for cosmetic or aesthetic purposes, application of mugagotoranoo or its extract for hair removal or hair growth inhibition, and use by estheticians, hairdressers, barbers, trimmers, etc. Is mentioned.

  In a preferred embodiment of the present invention, the mugkatrano or its extract is applied externally to the skin. Therefore, this invention also provides the skin external preparation which uses mukagotoranoo or its extract as an active ingredient. In addition, the present invention provides use of musgotrano or its extract for the manufacture of a skin external preparation. The present invention also provides the use of mugagotrano or an extract thereof for inhibiting hair growth or removing or removing hair by external application to the skin. In addition, the present invention provides a mugagotrano or extract thereof for use in the suppression of hair growth by external application to the skin, or for hair removal or hair removal.

  Mucagotorano or its extract can also be used for the manufacture of pharmaceuticals, quasi drugs, cosmetics or other compositions for hair growth inhibition or for hair removal or hair removal. The said pharmaceutical, quasi-drug, cosmetics, and a composition contain mukagotoranoo or its extract as an active ingredient for hair growth suppression or hair removal or hair removal. In addition, the pharmaceutical, quasi-drug, cosmetic and composition may be a pharmaceutically or cosmetically acceptable carrier or a pharmaceutically acceptable carrier as necessary, as long as the hair growth inhibitory action of mugagotrano or its extract is not lost. It may contain additives, or other active ingredients, pharmacological ingredients, cosmetic ingredients, and the like.

  Alternatively, mugagotrano or an extract thereof can be added to any pharmaceutical, quasi-drug, cosmetic, or other composition as a hair growth inhibiting component. For example, moisturizing and whitening skin care products, antiperspirants, deodorants, body soaps, etc., containing mugagotoranoo or an extract thereof as a hair growth inhibiting component can be produced.

  The pharmaceuticals, quasi-drugs, cosmetics and compositions containing musgotranoo or its extract can be prepared in any form, but preferably are external preparations. The external preparation may preferably be in the form of a skin external preparation for hair growth inhibition or hair removal or hair removal, such as an ointment, cream, lotion, gel, foam, patch, tape, sheet, spray and the like. Alternatively, the external preparation may be a moisturizing or whitening skin care product, an antiperspirant, a deodorant, a body soap or the like having a hair growth suppressing function.

  The content of mugagotoranoo or its extract in the above pharmaceutical products, quasi-drugs, cosmetics and compositions is preferably 0.0005 mass% or more, more preferably 0.001 mass in terms of dry mass of the extract. % Or more, more preferably 0.005 mass% or more, on the other hand, preferably 10 mass% or less, more preferably 1 mass% or less, still more preferably 0.1 mass% or less.

  In yet another aspect, the present invention provides a method for inhibiting hair growth in a subject. The present invention also provides a subject hair removal or hair removal method. The method includes administering to the subject an effective amount of mugagotrano or an extract thereof. The method may be a therapeutic method or a non-therapeutic method. The method may be an in vitro method.

  Examples of the target in the above method include animals for which hair growth suppression is desired and animals for which hair removal or hair removal is desired. The animal preferably includes humans or non-human mammals including dogs and cats, more preferably humans. Alternatively, examples of the object include tissues, organs, and cells having hair growth ability derived from the animals.

  In one embodiment, the method can be a non-therapeutic method. Non-therapeutic methods include a method of applying musgotrano or its extract to a human desiring hair removal, hair removal or hair growth suppression for cosmetic or aesthetic purposes, hair removal for cosmetic or aesthetic purposes, A method for applying mugagotoranoo or an extract thereof to a non-human mammal in which hair removal or hair growth inhibition is desired, and applying the mugagotranoo or an extract thereof to the above-mentioned object by an esthetician, hairdresser, barber, trimmer, etc. The method etc. are mentioned.

  An effective amount of administration in the above method may be an amount that can achieve inhibition of hair growth in the subject. Preferably, the effective amount may be an amount that can reduce the hair follicle elongation of the administration group to 95% or less, preferably 90% or less, more preferably 80% or less of the non-administration group. Also preferably, the effective amount may be an amount that can reduce the hair diameter of the administration group to 95% or less, preferably 90% or less, more preferably 80% or less of the non-administration group.

  The effective amount of administration can vary according to the subject's species, weight, sex, age, condition or other factors. The dose, route and interval of administration can be determined appropriately by those skilled in the art. A preferred route of administration is topical administration to the skin. The dose is preferably 0.001 mg or more, more preferably 0.005 mg or more, and preferably 100 mg or less, more preferably 50 mg per day for an adult (60 kg) per day in terms of dry matter of the extract of musgotranoo. It is as follows. For example, the extract of musgotranoo is suppressed to hair growth at a dose of 0.001 to 100 mg / day, preferably 0.005 to 50 mg / day in terms of dry matter, once, twice or three times a day. It is preferable to administer it to the skin at the desired site.

  The present invention also includes the following compositions, manufacturing methods, uses or methods as exemplary embodiments. However, the present invention is not limited to these embodiments.

<1> A hair growth inhibitor comprising musgotranoo or an extract thereof as an active ingredient.

<2> Use of mugagotrano or an extract thereof as a hair growth inhibitor.

<3> Use of mugkatrano or extract thereof for the production of a hair growth inhibitor.

<4> A hair remover or a hair remover comprising musgotranoo or its extract as an active ingredient.

<5> Use of Muskatoranoo or an extract thereof as a hair removal agent or a hair removal agent.

<6> Use of mugagotrano or an extract thereof for the production of a hair removal agent or a hair removal agent.

<7> Mucagotorano or extract thereof for use in hair growth inhibition.

<8> Mukagotoranoo or an extract thereof for use in hair removal or hair removal.

<9> A method for inhibiting hair growth, which comprises applying Mukagotorano or an extract thereof.

<10> A method of depilation or depilation comprising applying Mucagotorano or an extract thereof.

<11> A topical skin preparation containing musgotranoo or an extract thereof as an active ingredient.

<12> Use of mugagotorano or an extract thereof as an external preparation for skin.

<13> Use of musgotranoo or an extract thereof for producing a skin external preparation.

<14> Mucagotorano or extract thereof for use as a skin external preparation.

  <15> In the above items <1> to <3>, the hair growth inhibitor is preferably a skin external preparation.

  <16> In the above <4> to <6>, the hair removal agent or hair removal agent is preferably a skin external preparation.

  <17> In the above items <7> to <10>, the muggotrano or the extract thereof is preferably used externally on the skin.

  <18> In the above item <9> or <10>, the method is preferably a non-therapeutic method for hair removal, hair removal or hair growth suppression for cosmetic or aesthetic purposes.

  <19> In the above item <18>, the above-mentioned mugagotorano or an extract thereof is preferably applied to the skin of a site where hair growth of the target animal is desired to be suppressed by an esthetician, hairdresser, barber, trimmer, etc. Inhibits hair growth.

<20> In the above items <1> to <19>, the muggotrano is preferably a rhizome of mugagotrano.

<21> In the above items <1> to <19>, the above-mentioned mugagotorano or an extract thereof is preferably an extract of mugagotrano.

<22> In the above item <21>, the extract of Mukagotorano is preferably a rhizome extract of Mukagotorano.

<23> In the above items <1> to <22>, the extract of musgotranoo is preferably an extract of an aqueous solution of musgotranoo.

<24> In the above <23>, the ethanol concentration in the ethanol aqueous solution is preferably 20% by volume or more, more preferably 30% by volume or more, and preferably 99.9% by volume or less, more preferably 99%. .5% by volume or less, more preferably 95% by volume or less.

<25> In the above <23>, the ethanol concentration in the ethanol aqueous solution is preferably 20% by volume or more and 99.9% by volume or less, more preferably 20% by volume or more and 99.5% by volume or less, and further preferably 30% by volume. % To 95% by volume.

  EXAMPLES Hereinafter, an Example is shown and this invention is demonstrated more concretely.

Example 1 Evaluation of hair-loss action of test substance using pig isolated hair follicle (1) Preparation of extract of mugagotranoo 1000 ml of 50% (v / v) ethanol aqueous solution was added to 50 g of rhesus rhizome and soaked at room temperature for 19 days. . This was filtered to obtain a mugagotranoo extract. When this extract was concentrated, the solid content was 5.2 g. This extract was diluted with 50% (v / v) ethanol to give a 1% (w / v) solution.

(2) Addition to organ culture hair follicle The buttocks skin of pork for meat was cut into an appropriate size to remove excess adipose tissue. After dipping in the Hibiten solution for 20 minutes, it was washed 3 times with D-PBS (Ca and Mg free, GIBCO BRL 10% antibacterial agent included). After washing, sterilize gauze (white cross sterase) on a 10 cm petri dish and add an appropriate amount of PBS (Ca and Mg free, GIBCO BRL 1% antibacterial agent) to prevent skin dryness. placed. Next, using tweezers and a scalpel (FEATER No. 10) under the actual microscope, they were isolated one by one while being careful not to damage the hair follicles and collected in the medium. William's Medium E (SIGMA) was used as the medium. As additives, L-glutamine (GIBCO 2503) 2 mM, antibiotics (GIBKO 15240) 1% hydrocortisone (SIGMA) 10 μg / mL, and insulin (SIGMA) 0.01 μg / mL were added.
The isolated hair follicles were placed one by one in a 24-well plate containing the above medium and cultured under conditions of 37 ° C. and 5% CO ₂ . Photographs were taken at the start of culture and after 2 days of culture, and image analysis was performed. From the analysis results, the hair follicles elongated by 0.2 mm or more in 2 days were transferred to the medium to which the test substance was added. As the test substance, the extract of Mukatago noran prepared in (1) (final concentration 0.01% (w / v)) was used, and the same amount of 50% (v / v) ethanol solution was used as a control (control). .
Photographing and medium exchange were performed on the 0th, 4th, 6th, and 8th days after the start of culture in the test substance-added medium. Image analysis was performed from the photograph, and the amount of hair follicle elongation was measured.

  The results are shown in FIG. In the control group, hair follicle elongation (hair growth) was observed with the lapse of the culture time, while in the group added with mugagotrano, hair follicle elongation was significantly suppressed (non-paired student's- t-test, vs control).

Claims (2)

  A hair growth inhibitor comprising musgotranoo or its extract as an active ingredient. A hair removal or hair removal agent comprising musgotranoo or its extract as an active ingredient.

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