JP6180170B2 - Hair growth inhibitor - Google Patents

Description

  The present invention relates to a hair growth inhibitor.

  Biologically, hair or body hair protects important organs such as the head, chest, and limbs. However, in recent years, there is an increasing tendency that hair on limbs and the like is preferably not in terms of aesthetic appearance.

  As a method for removing body hair, a mechanical removal method using a tweezer, a shaver, a hair removal device, or the like, and a removal method by a chemical action using a hair removal agent or a hair removal agent are known. However, these known hair removal methods may involve physical or chemical irritation to the skin. Moreover, in the said known hair removal method, since the hair growth of the hair reproduced | regenerated after removal cannot be suppressed continuously, after a fixed period passes, the hair removal process must be performed again. Therefore, there is a demand for a hair growth inhibitor that can reduce the number of treatments and reduce labor by continuously and effectively suppressing the growth of body hair.

  Smoke trees (mangrove trees) and mangoes are both plants belonging to the Urushi family. Patent Document 1 describes that hair growth was induced by a hot water extract of a white bear. Hagmanoki is also known as an anti-inflammatory agent, wound healing agent, preservative, astringent, bile secretion promoter, antipyretic agent and the like (see Patent Document 1). Mango has long been used as an ingredient in edible and cosmetic products. Patent Document 2 describes a hair restorer containing an extract obtained by column purification of an aqueous mango acetone solution. Patent Document 3 describes a skin external preparation for acne and a lipase inhibitor containing a mango extract, and Patent Document 4 describes whitening containing mango germ oil obtained by fractionating and deodorizing mango seed nuclei. Cosmetics are described, and Patent Document 5 describes that skin lotion containing steam distilled water of mango has improved dry skin and skin gloss / tension.

JP 2010-513296 A JP 2003-063935 A JP 2006-298814 A Japanese Patent Laying-Open No. 2005-314286 JP 2001-226249 A

  The present invention relates to providing a hair growth inhibitor that effectively and continuously suppresses hair growth.

  As a result of searching for substances that suppress hair growth, the present inventors have found that smoke tree and mango, which have not been known to suppress hair growth, have an action of suppressing hair growth. .

That is, this invention provides the hair growth inhibitor which uses as an active ingredient at least 1 selected from the group which consists of smoke tree, mango, and those extracts.
The present invention also provides a hair removal or hair removal agent comprising as an active ingredient at least one selected from the group consisting of smoke tree, mango and extracts thereof.

  According to the present invention, it is possible to provide a hair removal agent, a hair removal agent, a skin external preparation and the like that are derived from plants and are safe for living bodies and have a high hair growth inhibitory effect.

Effect of smoke tree extract on hair growth. Effect of mango extract on hair growth.

  In the present specification, “hair growth inhibition” means an action of suppressing the growth of hair or hair follicles or an action of reducing hair diameter.

  In this specification, examples of the “hair” whose growth is suppressed include head hair, beard, and body hair other than head hair such as limbs and trunk hair, but hair other than beard and head hair is preferred.

  As used herein, “non-therapeutic” does not include medical practice, that is, does not include methods for surgery, treatment or diagnosis of humans, more specifically a doctor, or a health care professional or doctor's instructions. It is a concept that does not include a method for the recipient to perform surgery, treatment or diagnosis on humans.

  In the present specification, the “smoked tree” is also referred to as a hagmanoki and refers to Cotinus coggygria belonging to the genus Ursiaceae, and “mango” refers to Mangifera indica belonging to the genus Mango. In the present invention, the plant has any part thereof, for example, whole tree, leaf, stem, stem, bud, flower, bud, wood part, bark, root, rhizome, seed, fruit, or resin, or a combination thereof. Can be used. Among these, a preferable part is a leaf in the smoke tree and a seed in the mango. In the present invention, the above-mentioned plants may be used as they are, or may be used after being dried, or may be further used by cutting, crushing, crushing and exploiting them.

  The plant extract can be obtained by subjecting the plant part described above to the extraction step as it is or after drying, or further cutting, crushing, and pulverizing. As the plant extract, commercially available products may be used, or various solvent extracts obtained by conventional methods, or diluted solutions, concentrated solutions, dried powders, or pastes. It may be.

  As the solvent for extraction, either a polar solvent or a nonpolar solvent can be used. Specific examples of the solvent include, for example, water; monovalent, divalent or polyhydric alcohols; ketones such as acetone and methyl ethyl ketone; esters such as methyl acetate and ethyl acetate; Cyclic ethers; polyethers such as polyethylene glycol; saturated or unsaturated hydrocarbons; aromatic hydrocarbons such as benzene and toluene; halogenated hydrocarbons such as dichloromethane, chloroform, dichloroethane, and carbon tetrachloride; Pyridines; dimethyl sulfoxide; acetonitrile; carbon dioxide, supercritical carbon dioxide; fats and oils, waxes, other oils; and mixtures thereof. Preferable examples include water, alcohols and aqueous solutions thereof in terms of pharmacological activity and versatility.

  Examples of the alcohols include monohydric alcohols such as methanol, ethanol, propanol, butanol, amyl alcohol, hexanol, heptanol, octanol; ethylene glycol, propylene glycol, 1,3-butylene glycol, 1,4-butanediol. 2, dihydric alcohols such as 1,5-pentanediol and 1,6-hexanediol; trihydric or higher alcohols such as glycerin. Among these, monohydric alcohols and dihydric alcohols are preferable from the viewpoint of versatility. Preferably, the alcohol may be an alcohol having 1 to 10 carbon atoms, more preferably an alcohol having 1 to 4 carbon atoms.

  Preferable examples of the alcohols include methanol, ethanol, 1,3-butylene glycol, n-propanol, isopropanol, n-butanol, isobutanol, sec-butanol, t-butanol and the like.

  The concentration of alcohol in the aqueous solution of the alcohol is preferably 20% by volume or more, more preferably 30% by volume or more, and preferably 99.9% by volume or less, more preferably 99.5% by volume or less, Preferably it is 95 volume% or less. Alternatively, the concentration of the alcohol in the aqueous solution of the alcohol is preferably 20% to 99.9% by volume, more preferably 20% to 99.5% by volume, and even more preferably 30% to 95% by volume. It is as follows.

  Of the alcohols, ethanol is more preferable from the viewpoint of versatility. Therefore, more preferable solvents for preparing the plant extract include water, ethanol and an aqueous ethanol solution, and more preferably an aqueous ethanol solution.

  The ethanol concentration of the aqueous ethanol solution is preferably 20% by volume or more, more preferably 30% by volume or more, and preferably 99.9% by volume or less, more preferably 99.5% by volume or less, and still more preferably 95% by volume. % Or less. Alternatively, the ethanol concentration of the aqueous ethanol solution is preferably 20% to 99.9% by volume, more preferably 20% to 99.5% by volume, and still more preferably 30% to 95% by volume.

  As a usage-amount of the solvent in extraction, 1-100 mL is preferable with respect to 1 g of plants (dry mass conversion). The extraction conditions are not particularly limited as long as sufficient extraction can be performed. For example, the extraction time is preferably 1 minute or longer, more preferably 10 minutes or longer, on the other hand, preferably 2 months or shorter, more preferably 5 weeks or shorter. The extraction temperature is preferably 0 ° C. or higher, more preferably 5 ° C. or higher, and the solvent boiling point or lower is preferable. Usually, extraction is performed for a long time at a low temperature and for a short time at a high temperature. Examples of extraction conditions include 15 to 40 ° C. for 3 days to 5 weeks, but are not limited thereto, and can be appropriately selected by those skilled in the art.

  Extraction means for obtaining the plant extract is, for example, usual means such as solid-liquid extraction, liquid-liquid extraction, immersion, decoction, leaching, reflux extraction, Soxhlet extraction, ultrasonic extraction, microwave extraction, stirring, etc. Can be used. These extraction means may be combined. For example, dipping or solid-liquid extraction may be combined with liquid-liquid extraction, or when the extraction time is shortened, solid-liquid extraction with stirring may be performed. Furthermore, in order to prevent the oxidation of the extract, a means for extracting under a so-called non-oxidizing atmosphere while removing dissolved oxygen by bubbling degassing or inert gas such as nitrogen gas may be used in combination.

  The extract obtained as described above can be used as it is, but it can also be diluted, concentrated or freeze-dried, and / or prepared in a liquid, powder or paste form. Alternatively, treatment using activated carbon, adsorption resin, ion exchange resin, activated clay, silica gel or the like, which is generally used for production of plant extracts, can also be performed.

  In the present invention, any of the smoke tree, mango and their extract may be used alone or in a combination of two or more selected from the group consisting of the smoke tree, mango and their extract described above. May be used. The extract may be any one of the various solvent extracts described above or a combination of two or more of the above solvent extracts, but preferably includes an aqueous ethanol extract, and more preferably an aqueous ethanol extract. Here, the ethanol concentration in the aqueous ethanol solution used for extraction of smoke tree or mango is preferably 20% by volume or more, more preferably 30% by volume or more, and preferably 99.9% by volume or less, more preferably It may be 99.5% by volume or less, more preferably 95% by volume or less. Alternatively, the ethanol concentration in the ethanol aqueous solution used for extraction of smoke tree or mango is, for example, 20% to 99.9% by volume, 20% to 99.5% by volume, 20% to 95% by volume. % By volume or less, preferably 30% by volume or more and 99.9% by volume or less, more preferably 30% by volume or more and 99.5% by volume or less, more preferably 30% by volume or more and 95% by volume or less, and even more preferably. 40 volume% or more and 95 volume% or less is mentioned.

  The above smoke tree, mango or extract thereof can suppress hair growth in humans or non-human animals, or tissues, organs and cells derived therefrom, and thereby effective for hair removal, hair loss, etc. Can be demonstrated. For example, the smoke tree, mango or an extract thereof can remove hair from the skin by its hair growth inhibitory action. Alternatively, the above smoke tree, mango or extract thereof has a hair growth inhibitory action, thereby reducing the number and thickness of the hair, facilitating the hair removal and hair removal treatment, or delaying the hair growth after the treatment. Thus, the persistence of the hair removal and hair removal treatment can be increased, and the frequency of the necessary hair removal and hair removal treatment can be reduced.

Therefore, in one aspect, the present invention provides a hair growth inhibitor comprising as an active ingredient at least one selected from the group consisting of smoke tree, mango and extracts thereof. The present invention also provides a hair removal agent or hair removal agent comprising as an active ingredient at least one selected from the group consisting of smoke tree, mango and extracts thereof.
In another aspect, the present invention provides at least one use selected from the group consisting of a hair growth inhibitor, or a smoke tree, mango and extracts thereof for the manufacture of a hair removal or hair removal agent.
In one embodiment, the agent may consist essentially of at least one selected from the group consisting of smoke tree, mango and extracts thereof.

In yet another aspect, the present invention provides at least one use selected from the group consisting of smoke tree, mango and extracts thereof for hair growth inhibition or for hair removal or hair removal.
In yet another aspect, the present invention provides at least one selected from the group consisting of smoke tree, mango and extracts thereof for use in hair growth inhibition or hair removal or hair removal.
The use may be a therapeutic use but may also be a non-therapeutic use. Non-therapeutic uses include hair removal for cosmetic or aesthetic purposes, at least one application selected from the group consisting of smoke tree, mango and extracts thereof for hair removal or hair growth inhibition, and estheticians , Use by hairdressers, barbers, trimmers, etc.

  In a preferred embodiment of the present invention, the smoke tree, mango or extract thereof is applied topically to the skin.

  Smoked trees, mangoes or extracts thereof may also be used for the manufacture of pharmaceuticals, quasi drugs, cosmetics, or other compositions for hair growth inhibition or for hair removal or hair removal. it can. The medicinal product, quasi-drug, cosmetic and composition are at least one selected from the group consisting of smoke tree, mango and extracts thereof, for hair growth inhibition, or for hair removal or hair removal. Contains as an active ingredient. Furthermore, the pharmaceutical, quasi-drug, cosmetic and composition are pharmaceutically or cosmetically acceptable carriers as needed, as long as the hair growth inhibitory action of the plant or extract thereof is not lost. Or additives, or other active ingredients, pharmacological ingredients, cosmetic ingredients, and the like.

  Alternatively, smoke tree, mango or extracts thereof can be added to any pharmaceutical, quasi-drug, cosmetic or other composition as a hair growth inhibiting ingredient. For example, a moisturizing and whitening skin care product, an antiperspirant, a deodorant, a body soap, etc., containing at least one selected from the group consisting of smoke tree, mango and extracts thereof as a hair growth inhibiting component be able to.

  The pharmaceuticals, quasi-drugs, cosmetics and compositions containing the above plants or their extracts can be prepared in any form, but are preferably external preparations. The external preparation may preferably be in the form of a skin external preparation for hair growth inhibition or hair removal or hair removal, such as an ointment, cream, lotion, gel, foam, patch, tape, sheet, spray and the like. Alternatively, the external preparation may be a moisturizing or whitening skin care product, an antiperspirant, a deodorant, a body soap or the like having a hair growth suppressing function.

  The content of the plant or the extract thereof in the pharmaceutical, quasi-drug, cosmetic and composition is preferably 0.0005% by mass or more, more preferably 0.001 in terms of the dry mass of the extract. On the other hand, it is preferably 10% by mass or less, more preferably 1% by mass or less, and further preferably 0.1% by mass or less.

  In yet another aspect, the present invention provides a method for inhibiting hair growth in a subject. The present invention also provides a subject hair removal or hair removal method. The method comprises administering to the subject an effective amount of at least one selected from the group consisting of smoke tree, mango and extracts thereof. The method may be a therapeutic method or a non-therapeutic method. The method may be an in vitro method.

  Examples of the target in the above method include animals for which hair growth suppression is desired and animals for which hair removal or hair removal is desired. The animal preferably includes humans or non-human mammals including dogs and cats, more preferably humans. Alternatively, examples of the object include tissues, organs, and cells having hair growth ability derived from the animals.

  In one embodiment, the method can be a non-therapeutic method. Non-therapeutic methods include humans who desire hair removal, hair removal or hair growth inhibition for cosmetic or aesthetic purposes, or hair removal, hair removal or hair growth inhibition for cosmetic or aesthetic purposes. By applying to a non-human mammal at least one selected from the group consisting of smoke tree, mango and extracts thereof, estheticians, hairdressers, barbers, trimmers, etc., the smoke tree, mango and And a method of applying at least one selected from the group consisting of these extracts.

  An effective amount of administration in the above method may be an amount that can achieve inhibition of hair growth in the subject. Preferably, the effective amount may be an amount that can reduce the hair follicle elongation of the administration group to 95% or less, preferably 90% or less, more preferably 80% or less of the non-administration group. Also preferably, the effective amount may be an amount that can reduce the hair diameter of the administration group to 95% or less, preferably 90% or less, more preferably 80% or less of the non-administration group.

  The effective amount of administration can vary according to the subject's species, weight, sex, age, condition or other factors. The dose, route and interval of administration can be determined appropriately by those skilled in the art. A preferred route of administration is topical administration to the skin. The dose is preferably 0.001 mg or more, more preferably 0.005 mg or more, and preferably 100 mg or less, more preferably, per day for an adult (60 kg) per day in terms of dry matter of the plant extract. 50 mg or less. For example, the above-mentioned plant extract is used as a dry matter at a dose of 0.001 to 100 mg / day, preferably 0.005 to 50 mg / day once, twice or three times a day. It is preferable to administer to the skin of the site to be suppressed.

  The present invention also includes the following compositions, manufacturing methods, uses or methods as exemplary embodiments. However, the present invention is not limited to these embodiments.

<1> A hair growth inhibitor comprising as an active ingredient at least one selected from the group consisting of smoke tree, mango and extracts thereof.

<2> Use of at least one selected from the group consisting of smoke tree, mango and extracts thereof as a hair growth inhibitor.

<3> Use of at least one selected from the group consisting of smoke tree, mango and extracts thereof for the production of a hair growth inhibitor.

<4> A hair remover or a hair remover comprising as an active ingredient at least one selected from the group consisting of smoke tree, mango and extracts thereof.

<5> Use of at least one selected from the group consisting of smoke tree, mango and extracts thereof as a hair remover or hair remover.

<6> Use of at least one selected from the group consisting of smoke tree, mango and extracts thereof for the production of a hair removal agent or a hair removal agent.

<7> At least one selected from the group consisting of smoke tree, mango and extracts thereof for use in hair growth inhibition.

<8> At least one selected from the group consisting of smoke tree, mango and extracts thereof for use in hair removal or hair removal.

<9> A method for inhibiting hair growth, comprising applying at least one selected from the group consisting of smoke tree, mango and extracts thereof.

<10> A hair removal or hair removal method comprising applying at least one selected from the group consisting of smoke tree, mango and extracts thereof.

  <11> In the above items <1> to <3>, the hair growth inhibitor is preferably a skin external preparation.

  <12> In the above items <4> to <6>, the hair removal agent or hair removal agent is preferably a skin external preparation.

  <13> In the above items <7> to <10>, at least one selected from the group consisting of the smoke tree, mango and extracts thereof is preferably applied to the skin.

  <14> In the above <9> or <10>, the method is preferably a non-therapeutic method for hair removal, hair removal or hair growth suppression for cosmetic or aesthetic purposes.

  <15> In the above item <14>, at least one selected from the group consisting of the smoke tree, mango, and an extract thereof is preferably used for hair growth of the target animal by an esthetician, hairdresser, barber, trimmer, or the like. It is applied to the skin of the site to be suppressed to suppress the hair growth at the site.

<16> In the above items <1> to <15>, the smoke tree is preferably a smoke tree leaf, and the mango is preferably a mango seed.

<17> In the above items <1> to <15>, at least one selected from the group consisting of the smoke tree, mango, and an extract thereof is selected from the group consisting of the smoke tree extract and the mango extract. At least one.

<18> In the above item <17>, the smoke tree extract is preferably a smoke tree leaf extract, and the mango extract is preferably a mango seed extract.

<19> In the above items <1> to <18>, the smoke tree extract is preferably a smoke tree ethanol aqueous solution extract, and the mango extract is preferably a mango ethanol aqueous solution extract.

<20> In the above item <19>, the ethanol concentration in the aqueous ethanol solution is preferably 20% by volume or more, more preferably 30% by volume or more, and preferably 99.9% by volume or less, more preferably 99%. .5% by volume or less, more preferably 95% by volume or less.

<21> In the above item <19>, the ethanol concentration in the aqueous ethanol solution is preferably 20% to 99.9% by volume, 20% to 99.5% by volume, or 20% to 95% by volume. More preferably, it is 30 volume% or more and 99.9 volume% or less, More preferably, it is 30 volume% or more and 99.5 volume% or less, More preferably, it is 30 volume% or more and 95 volume% or less, Preferably, it is 40 volume% or more and 95 volume% or less.

  EXAMPLES Hereinafter, an Example is shown and this invention is demonstrated more concretely.

Example 1 Evaluation of Barrier Action of Test Substance Using Pig Isolated Hair Follicle (1) Preparation of Extract Preparation of Smoke Tree Extract Add 50 mL of 50% ethanol aqueous solution to 50 g of smoke tree leaf (purchased from Montage Herbs) at room temperature. Soaked for 15 days. This was filtered to obtain a smoke tree extract. When this smoke tree extract was concentrated, the solid content was 10.9 g. This extract was diluted with 50% ethanol to give a 1% (w / v) solution.

Preparation of Mango Extract To 50 g of pulverized mango seed (purchased from Shinwa Bussan), 500 mL of 50% (v / v) ethanol aqueous solution was added and immersed at room temperature for 20 days. This was filtered to obtain a mango extract. When the mango extract was concentrated, the solid content was 5.8 g. This extract was diluted with 50% (v / v) ethanol to give a 1% (w / v) solution.

(2) Addition to organ culture hair follicle The buttocks skin of pork for meat was cut into an appropriate size to remove excess adipose tissue. After dipping in the Hibiten solution for 20 minutes, it was washed 3 times with D-PBS (Ca and Mg free, GIBCO BRL 10% antibacterial agent included). After washing, sterilize gauze (white cross sterase) on a 10 cm petri dish and add an appropriate amount of PBS (Ca and Mg free, GIBCO BRL 1% antibacterial agent) to prevent skin dryness. placed. Next, using tweezers and a scalpel (FEATER No. 10) under the actual microscope, they were isolated one by one while being careful not to damage the hair follicles and collected in the medium. William's Medium E (SIGMA) was used as the medium. As additives, L-glutamine (GIBCO 2503) 2 mM, antibiotics (GIBKO 15240) 1% hydrocortisone (SIGMA) 10 μg / mL, and insulin (SIGMA) 0.01 μg / mL were added.
The isolated hair follicles were placed one by one in a 24-well plate containing the above medium and cultured under conditions of 37 ° C. and 5% CO ₂ . Photographs were taken at the start of culture and after 2 days of culture, and image analysis was performed. From the analysis results, the hair follicles elongated by 0.2 mm or more in 2 days were transferred to the medium to which the test substance was added. Each extract (final concentration 0.01% (w / v)) prepared in (1) was used as a test substance, and the same amount of 50% (v / v) ethanol solution was used as a control (control).
After the start of culture in the test substance-added medium, photography and medium exchange were performed on the 0, 2, 5, 7, and 9 days for the evaluation of the smoke tree extract and on the 0, 4, 6, and 8 days for the evaluation of the mango extract. Went. Image analysis was performed from the photograph, and the amount of hair follicle elongation was measured.

  The results are shown in FIGS. In the control group, hair follicle elongation (hair growth) was observed with the passage of culture time, while in the smoke tree added group and mango added group, hair follicle elongation was significantly suppressed (non-paired). student's-t-test, vs control).

Claims (4)

Hair growth inhibitor comprising as an active ingredient at least one selected from the group consisting of Ma Ngo and extracts thereof. As an active ingredient the aqueous ethanol solution extract of Ma Ngo, hair growth inhibitor according to claim 1. Hair removal or hair loss agent comprising as an active ingredient at least one selected from the group consisting of Ma Ngo and extracts thereof. As an active ingredient the aqueous ethanol solution extract of Ma Ngo, depilatory or hair loss agent according to claim 3, wherein.

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