JP6165891B2 - 流路制御方法および細胞培養装置 - Google Patents
流路制御方法および細胞培養装置 Download PDFInfo
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- F16K7/00—Diaphragm valves or cut-off apparatus, e.g. with a member deformed, but not moved bodily, to close the passage ; Pinch valves
- F16K7/02—Diaphragm valves or cut-off apparatus, e.g. with a member deformed, but not moved bodily, to close the passage ; Pinch valves with tubular diaphragm
- F16K7/04—Diaphragm valves or cut-off apparatus, e.g. with a member deformed, but not moved bodily, to close the passage ; Pinch valves with tubular diaphragm constrictable by external radial force
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Description
本願はX≦2Nを満たすX本の流路の流路制御方法であって、N個の弁を用いて、同時に複数の流路を選択的に開閉制御することにより、X通りの流路を選択する。
(b)はその詳細を図示したものである。(i)は全てのピンチ弁がOFFの状態で、このとき、iのチューブが開放状態である。ここで、Aのピンチ弁をONすると、(ii)のようになり、iのチューブは閉塞し、iiのチューブが開放状態となる。同様にして、3箇所のピンチ弁を制御して、8本のチューブのいずれかを開放にすることができる。
なお、本発明で取り扱う流路は円管構造に限らない。チューブ以外に、フィルムを張り合わせて成形した流路等にも適用できる。ピンチ弁のピンチ力に対し変形可能な、弾性ないし可撓性を備えた流路であればよい。
培養容器から複数の回収物を分離したい場合、もしこれらの回収物にキャリーオーバーが全くあってはならない場合、(f)のように回収側で分けてもよい。P個の培養容器13と、Q個の回収バッグ15が、それぞれ(P×Q)本の個別流路24で接続され、その箇所に流路選択機構20を設置すればよい。上流側を一つにまとめれば、駆動源は一つで済む。
特許文献1に記載の多連切替え弁は、すべり弁同士を押し当てて切替えることで、リークなく切替えているが、押し当てている構造上、この部分からのリークが0ではない。リークを極力抑えるためには、メンテナンスが非常に重要であり、かつ手間であった。
Claims (9)
- 弾性または可撓性を備えたX本(X≧3)の流路のうちいずれか1本を開放する流路制御装置であって、
X本(X≧3)の流路と、
X≦2Nを満たす最小のN個の弁を備え、
前記弁は開放側と閉塞側の2つの流路通過部位を有し、前記開放側と前記閉塞側はそれぞれ同時に複数の流路が通過可能であって、
1個の前記弁は、流路通過部位にX本の全ての流路が通過し、
前記N個の弁すべてを同時に制御することにより、前記X本の流路のうちいずれか1本を開放する流路制御装置。 - 請求項1の流路制御装置において、
前記N個の制御弁の駆動源がOFFとなった場合に、N個の制御弁の全てで流路が開放状態になる組合せでは使用しない、流路制御装置。 - 流体の導入口と排出口を持つ、密閉系の培養容器に、
培養に必要な液体類を収容する供給バッグと、
使用後の液体を回収する回収バッグと、が接続され、
閉鎖された系を形成し、前記閉鎖系内で細胞の培養を行う、細胞培養装置であって、
前記供給バッグは、X≦2Lを満たすX個あって、これらが1つの共通流路に並列に接続された、請求項1の流路制御装置を用いる細胞培養装置。 - 流体の導入口と排出口を持つ、密閉系の培養容器に、
培養に必要な液体類を収容する供給バッグと、
使用後の液体を回収する回収バッグと、が接続され、
閉鎖された系を形成し、前記閉鎖系内で細胞の培養を行う、細胞培養装置であって、前記培養容器は、X≦2Mを満たすX個あって、これらが導入口側および排出口側のそれぞれの共通流路に並列に接続された請求項1の流路制御装置を用いる細胞培養装置。 - 流体の導入口と排出口を持つ、密閉系の培養容器に、
培養に必要な液体類を収容する供給バッグと、
使用後の液体を回収する回収バッグと、が接続され、
閉鎖された系を形成し、前記閉鎖系内で細胞の培養を行う、細胞培養装置であって、
前記回収バッグは、X≦2Nを満たすX個あって、これらが1つの流路に並列に接続された請求項1の流路制御装置を用いる細胞培養装置。 - 流体の導入口と排出口を持つ、密閉系の培養容器に、
培養に必要な液体類を収容する供給バッグと、
使用後の液体を回収する回収バッグと、が接続され、
閉鎖された系を形成し、前記閉鎖系内で細胞の培養を行う、細胞培養装置であって、
前記供給バッグおよび前記培養容器が、それぞれA個、B個あって、X≦2Pを満たすX本の個別流路で接続された請求項1の流路制御装置を用いる細胞培養装置。 - 流体の導入口と排出口を持つ、密閉系の培養容器に、
培養に必要な液体類を収容する供給バッグと、
使用後の液体を回収する回収バッグと、が接続され、
閉鎖された系を形成し、前記閉鎖系内で細胞の培養を行う、細胞培養装置であって、
前記培養容器および前記回収バッグが、それぞれC個、D個あって、X≦2Oを満たすX本の個別流路で接続された請求項1の流路制御装置を用いる細胞培養装置。 - 請求項3乃至5の細胞培養装置であって、共通流路部に液体駆動源を備える細胞培養装置。
- 請求項3乃至7の細胞培養装置であって、請求項2に記載の流路制御装置を用いる細胞培養装置。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
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JP2014012071 | 2014-01-27 | ||
JP2014012071 | 2014-01-27 | ||
PCT/JP2014/084015 WO2015111347A1 (ja) | 2014-01-27 | 2014-12-24 | 流路制御方法および細胞培養装置 |
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JPWO2015111347A1 JPWO2015111347A1 (ja) | 2017-03-23 |
JP6165891B2 true JP6165891B2 (ja) | 2017-07-19 |
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JP2015558762A Expired - Fee Related JP6165891B2 (ja) | 2014-01-27 | 2014-12-24 | 流路制御方法および細胞培養装置 |
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Country | Link |
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US (1) | US10208277B2 (ja) |
JP (1) | JP6165891B2 (ja) |
WO (1) | WO2015111347A1 (ja) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2019155033A1 (en) * | 2018-02-09 | 2019-08-15 | General Electric Company | Bioprocessing vessel |
US10889792B2 (en) | 2018-02-09 | 2021-01-12 | Global Life Sciences Solutions Usa Llc | Cell expansion vessel systems and methods |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS6093356A (ja) | 1983-10-28 | 1985-05-25 | Hitachi Ltd | 分注装置 |
JP2548732B2 (ja) | 1987-07-03 | 1996-10-30 | 株式会社日立製作所 | 生化学自動分析装置 |
US6079691A (en) * | 1997-03-18 | 2000-06-27 | Dragone; Rocco V. | Pinch valve assembly |
JP2008054603A (ja) * | 2006-08-31 | 2008-03-13 | Shibuya Kogyo Co Ltd | 細胞培養装置 |
JP5140095B2 (ja) | 2010-01-13 | 2013-02-06 | 株式会社日立製作所 | 自動培養装置、及び培養容器設置方法 |
EP2604680A4 (en) | 2010-08-12 | 2015-06-24 | Hitachi Ltd | AUTOMATIC CULTURE DEVICE |
KR101589356B1 (ko) | 2011-04-13 | 2016-01-27 | 가부시키가이샤 히타치세이사쿠쇼 | 세포 배양 장치 및 반송 장치 |
JP5814594B2 (ja) * | 2011-04-13 | 2015-11-17 | 株式会社日立製作所 | 細胞培養装置 |
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2014
- 2014-12-24 WO PCT/JP2014/084015 patent/WO2015111347A1/ja active Application Filing
- 2014-12-24 JP JP2015558762A patent/JP6165891B2/ja not_active Expired - Fee Related
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US10208277B2 (en) | 2019-02-19 |
WO2015111347A1 (ja) | 2015-07-30 |
JPWO2015111347A1 (ja) | 2017-03-23 |
US20160319233A1 (en) | 2016-11-03 |
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