JP6140182B2 - 改善された活性を有するｄｎａポリメラーゼ - Google Patents

改善された活性を有するｄｎａポリメラーゼ Download PDF

Info

Publication number
JP6140182B2
JP6140182B2 JP2014545132A JP2014545132A JP6140182B2 JP 6140182 B2 JP6140182 B2 JP 6140182B2 JP 2014545132 A JP2014545132 A JP 2014545132A JP 2014545132 A JP2014545132 A JP 2014545132A JP 6140182 B2 JP6140182 B2 JP 6140182B2
Authority
JP
Japan
Prior art keywords
dna polymerase
amino acid
seq
polymerase
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2014545132A
Other languages
English (en)
Japanese (ja)
Other versions
JP2015505674A (ja
Inventor
バウアー キース
バウアー キース
ダブリュ．マイヤーズ トーマス
ダブリュ．マイヤーズ トーマス
スコ ショーン
スコ ショーン
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of JP2015505674A publication Critical patent/JP2015505674A/ja
Application granted granted Critical
Publication of JP6140182B2 publication Critical patent/JP6140182B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase
JP2014545132A 2011-12-08 2012-12-04 改善された活性を有するｄｎａポリメラーゼ Active JP6140182B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161568375P 2011-12-08 2011-12-08
US61/568,375 2011-12-08
PCT/EP2012/004992 WO2013083263A1 (en) 2011-12-08 2012-12-04 Dna polymerases with improved activity

Publications (2)

Publication Number Publication Date
JP2015505674A JP2015505674A (ja) 2015-02-26
JP6140182B2 true JP6140182B2 (ja) 2017-05-31

Family

ID=47278761

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2014545132A Active JP6140182B2 (ja) 2011-12-08 2012-12-04 改善された活性を有するｄｎａポリメラーゼ

Country Status (6)

Country Link
US (3) US8759063B2 (zh)
EP (1) EP2788479B1 (zh)
JP (1) JP6140182B2 (zh)
CN (1) CN103987844B (zh)
ES (1) ES2569723T3 (zh)
WO (1) WO2013083263A1 (zh)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2561885T3 (es) 2011-04-11 2016-03-01 F. Hoffmann-La Roche Ag ADN polimerasas de actividad mejorada
EP2788479B1 (en) 2011-12-08 2016-02-10 Roche Diagnostics GmbH Dna polymerases with improved activity
JP6144697B2 (ja) 2011-12-08 2017-06-07 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 改善された活性を有するｄｎａポリメラーゼ
JP6356694B2 (ja) 2012-12-13 2018-07-11 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 改善された活性を有するｄｎａポリメラーゼ
CN106399299B (zh) * 2016-09-29 2019-01-29 华南理工大学 一种通过点突变提高大片段嗜热脂肪地芽孢杆菌dna聚合酶活性的方法及应用
EP3768832B1 (en) * 2018-03-21 2023-11-29 F. Hoffmann-La Roche AG Dna polymerases for efficient and effective incorporation of methylated-dntps
US11384376B2 (en) 2018-05-31 2022-07-12 Roche Molecular Systems, Inc. Reagents and methods for post-synthetic modification of nucleic acids
IL302243A (en) * 2020-10-23 2023-06-01 Scripps Research Inst Reverse transcription of polynucleotides that include unnatural nucleotides
WO2022115822A2 (en) * 2020-11-03 2022-06-02 Northwestern University Methods for full-length reverse transcription pcr of long rnas containing modified ribonucleosides using a thermostable reverse transcriptase
CN114574465B (zh) * 2022-05-09 2022-08-05 上海众启生物科技有限公司 一种聚合酶突变体及其应用

Family Cites Families (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4666848A (en) 1984-08-31 1987-05-19 Cetus Corporation Polypeptide expression using a portable temperature sensitive control cassette with a positive retroregulatory element
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5079352A (en) 1986-08-22 1992-01-07 Cetus Corporation Purified thermostable enzyme
US5693502A (en) 1990-06-11 1997-12-02 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand inhibitors to DNA polymerases
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
DE69132477T2 (de) 1990-09-28 2001-06-13 Hoffmann La Roche Gereinigte thermostabile nukleinsäurepolymerase aus thermosipho africanus
JP2709311B2 (ja) 1990-09-28 1998-02-04 エフ．ホフマン−ラ ロシュ アクチェンゲゼルシャフト 熱安定性ｄｎａポリメラーゼの５→３′のエキソヌクレアーゼ突然変異
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
US5948614A (en) 1995-09-08 1999-09-07 Life Technologies, Inc. Cloned DNA polymerases from thermotoga maritima and mutants thereof
EP0834569A1 (en) 1996-10-03 1998-04-08 Roche Diagnostics GmbH Thermostable DNA polymerase from carboxydothermus hydrogenoformans
US6020128A (en) 1997-06-10 2000-02-01 United States Of Ameria DNA polymerase from Treponema pallidum
US6228628B1 (en) 1997-07-09 2001-05-08 Roche Molecular Systems Mutant chimeric DNA polymerase
CA2243985C (en) 1997-09-11 2004-08-10 F. Hoffmann-La Roche Ag Thermostable dna polymerases incorporating nucleoside triphosphates labeled with fluorescein family dyes
EP0921196A1 (en) 1997-12-02 1999-06-09 Roche Diagnostics GmbH Modified DNA-polymerase from carboxydothermus hydrogenoformans and its use for coupled reverse transcription and polymerase chain reaction
EP1088891B1 (en) 1999-09-28 2005-01-12 Roche Diagnostics GmbH Thermostable enzyme promoting the fidelity of thermostable DNA polymerases - for improvement of nucleic acid synthesis and amplification in vitro
US6329178B1 (en) 2000-01-14 2001-12-11 University Of Washington DNA polymerase mutant having one or more mutations in the active site
JP2001269188A (ja) 2000-03-11 2001-10-02 Roche Diagnostics Gmbh Ｐｃｒにおける性能が向上したｂ型ｄｎａポリメラーゼ変異体
US7179590B2 (en) 2000-04-18 2007-02-20 Roche Molecular Systems, Inc High temperature reverse transcription using mutant DNA polymerases
AU2002316586A1 (en) * 2001-07-06 2003-01-21 Amersham Biosciences Corp Novel dna polymerases having amino acid substitutions and homologs thereof
AU2002366438A1 (en) 2001-09-14 2003-09-02 Applera Corporation Thermus scotoductus nucleic acid polymerases
WO2003025132A2 (en) 2001-09-14 2003-03-27 Invitrogen Corporation Dna polymerases and mutants thereof
CA2409775C (en) 2001-12-03 2010-07-13 F. Hoffmann-La Roche Ag Reversibly modified thermostable enzymes for dna synthesis and amplification in vitro
AU2003244340A1 (en) 2002-02-05 2003-09-02 Indian Institute Of Technology Method for specific integration of t7 rna polymerase gene in the chromosome of corynebacterial and the resultant corynebacteria-t7 promoter based shuttle vector system.
US7148049B2 (en) 2002-04-02 2006-12-12 Roche Molecular Systems, Inc. Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity
WO2003102213A2 (en) 2002-05-31 2003-12-11 University Of Washington Error-prone dna polymerase i mutants and methods for targeted random mutagenesis in continuous culture using error-prone dna polymerase i mutants
US7572581B2 (en) 2003-06-30 2009-08-11 Roche Molecular Systems, Inc. 2′-terminator nucleotide-related methods and systems
US7947817B2 (en) 2003-06-30 2011-05-24 Roche Molecular Systems, Inc. Synthesis and compositions of 2'-terminator nucleotides
DK2145956T3 (da) 2003-11-03 2015-09-21 Medical Res Council Polymerase
US7417133B2 (en) 2004-02-27 2008-08-26 Institut Pasteur Methods for obtaining thermostable enzymes, DNA polymerase I variants from Thermus aquaticus having new catalytic activities, methods for obtaining the same, and applications of the same
US20060172324A1 (en) 2005-01-28 2006-08-03 Roche Molecular Systems, Inc. Methods of genotyping using differences in melting temperature
WO2007117331A2 (en) 2005-12-22 2007-10-18 Ge Healthcare Bio-Sciences Corp. Novel dna polymerase from thermoanaerobacter tengcongenesis
AU2007296180A1 (en) 2006-09-14 2008-03-20 Dna Polymerase Technology Inc. Use of Taq polymerase mutant enzymes for DNA amplification in the presence of PCR inhibitors
US8962293B2 (en) 2006-10-18 2015-02-24 Roche Molecular Systems, Inc. DNA polymerases and related methods
US8208381B2 (en) 2007-07-27 2012-06-26 Eg Innovations Pte. Ltd. Root-cause approach to problem diagnosis in data networks
US9315787B2 (en) 2011-01-14 2016-04-19 Kapa Biosystems, Inc. Modified DNA polymerases for improved amplification
JP6224613B2 (ja) 2011-12-08 2017-11-01 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 改善された活性を有するｄｎａポリメラーゼ
EP2788479B1 (en) 2011-12-08 2016-02-10 Roche Diagnostics GmbH Dna polymerases with improved activity

Also Published As

Publication number Publication date
US9951320B2 (en) 2018-04-24
JP2015505674A (ja) 2015-02-26
ES2569723T3 (es) 2016-05-12
CN103987844A (zh) 2014-08-13
EP2788479A1 (en) 2014-10-15
WO2013083263A1 (en) 2013-06-13
US20130149747A1 (en) 2013-06-13
US8759063B2 (en) 2014-06-24
US20140295500A1 (en) 2014-10-02
EP2788479B1 (en) 2016-02-10
US9428782B2 (en) 2016-08-30
WO2013083263A8 (en) 2014-05-01
CN103987844B (zh) 2016-01-20
US20170029791A1 (en) 2017-02-02

Similar Documents

Publication Publication Date Title
US10294462B2 (en) DNA polymerases with improved activity
JP6356694B2 (ja) 改善された活性を有するｄｎａポリメラーゼ
US10597644B2 (en) DNA polymerases with improved activity
US10487315B2 (en) DNA polymerases with improved activity
JP6140182B2 (ja) 改善された活性を有するｄｎａポリメラーゼ
US9738877B2 (en) DNA polymerases with improved activity

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20151203

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20161017

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20161025

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20170125

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20170404

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20170428

R150 Certificate of patent or registration of utility model

Ref document number: 6140182

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250