JP6118262B2 - 乾燥試料から遺伝子材料を電気溶出する方法 - Google Patents

乾燥試料から遺伝子材料を電気溶出する方法 Download PDF

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Publication number
JP6118262B2
JP6118262B2 JP2013544728A JP2013544728A JP6118262B2 JP 6118262 B2 JP6118262 B2 JP 6118262B2 JP 2013544728 A JP2013544728 A JP 2013544728A JP 2013544728 A JP2013544728 A JP 2013544728A JP 6118262 B2 JP6118262 B2 JP 6118262B2
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Prior art keywords
solid medium
dna
genetic material
sample
electroelution
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JP2013544728A
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Japanese (ja)
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JP2014502495A5 (https=
JP2014502495A (ja
Inventor
ファインハウト，エリン・ジーン
ネルソン，ジョン・リチャード
スプーナー，パトリック・マックコイ
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General Electric Co
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General Electric Co
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2013544728A 2010-12-17 2011-12-14 乾燥試料から遺伝子材料を電気溶出する方法 Expired - Fee Related JP6118262B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12/972,236 US20120152743A1 (en) 2010-12-17 2010-12-17 Method for electroeluting genetic material from dried samples
US12/972,236 2010-12-17
PCT/US2011/064823 WO2012082849A1 (en) 2010-12-17 2011-12-14 Method for electroeluting genetic material from dried samples

Publications (3)

Publication Number Publication Date
JP2014502495A JP2014502495A (ja) 2014-02-03
JP2014502495A5 JP2014502495A5 (https=) 2016-04-28
JP6118262B2 true JP6118262B2 (ja) 2017-04-19

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JP2013544728A Expired - Fee Related JP6118262B2 (ja) 2010-12-17 2011-12-14 乾燥試料から遺伝子材料を電気溶出する方法

Country Status (4)

Country Link
US (1) US20120152743A1 (https=)
EP (1) EP2652175B1 (https=)
JP (1) JP6118262B2 (https=)
WO (1) WO2012082849A1 (https=)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9040679B2 (en) 2012-04-30 2015-05-26 General Electric Company Methods and compositions for extraction and storage of nucleic acids
US9040675B2 (en) 2012-04-30 2015-05-26 General Electric Company Formulations for nucleic acid stabilization on solid substrates
US9480966B2 (en) 2012-04-30 2016-11-01 General Electric Company Substrates and methods for collection, stabilization and elution of biomolecules
US9044738B2 (en) 2012-04-30 2015-06-02 General Electric Company Methods and compositions for extraction and storage of nucleic acids
US9399986B2 (en) 2012-07-31 2016-07-26 General Electric Company Devices and systems for isolating biomolecules and associated methods thereof
WO2014035547A2 (en) * 2012-08-30 2014-03-06 General Electric Company Methods of isolating nucleic acids under reduced degradation condition
US9999856B2 (en) 2013-07-26 2018-06-19 General Electric Company Methods for electroelution of biomolecules
US9333463B2 (en) 2013-07-26 2016-05-10 General Electric Company Devices and systems for elution of biomolecules
ES2818569T3 (es) 2015-09-09 2021-04-13 Drawbridge Health Inc Métodos para la recopilación, estabilización y conservación de muestras
GB2590814B (en) 2017-01-10 2021-11-03 Drawbridge Health Inc Devices, systems, and methods for sample collection

Family Cites Families (18)

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US5187083A (en) * 1990-11-13 1993-02-16 Specialty Laboratories, Inc. Rapid purification of DNA
FR2726286B1 (fr) * 1994-10-28 1997-01-17 Genset Sa Procede d'amplification d'acides nucleiques en phase solide et trousse de reactifs utile pour la mise en oeuvre de ce procede
AR021833A1 (es) * 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US6221635B1 (en) * 1999-05-06 2001-04-24 The Wistar Institute Methods for solid-phase amplification of DNA template (SPADT) using multiarrays
JP4071907B2 (ja) * 1999-11-29 2008-04-02 オリンパス株式会社 自動核酸検査装置
US6503716B1 (en) * 2000-11-28 2003-01-07 Pe Corporation (Ny) Compositions and methods for extracting a nucleic acid
US20040219545A1 (en) * 2001-04-13 2004-11-04 Rando Robert F. Methods for identifying small molecules that bind specific rna structural motifs
CA2444087A1 (en) * 2001-04-12 2002-10-24 Cropdesign N.V. Plant growth regulating genes, proteins and uses thereof
GB0127809D0 (en) * 2001-11-20 2002-01-09 Dna Res Innovations Ltd Purification of nucleic acid
JP5441290B2 (ja) * 2002-10-04 2014-03-12 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション 核酸精製システムの媒体上に核酸を保存するツールとして、化学物質を用いる方法および材料
EP1581646A4 (en) * 2002-10-07 2006-07-05 Marligen Biosciences Inc EXTRACTION OF DNA FROM BIOLOGICAL SAMPLES
US20040241734A1 (en) * 2003-04-28 2004-12-02 Exagen Diagnostics Methods for in situ hybridization without the need for competitior DNA
EP1633893B1 (en) * 2003-05-19 2012-01-25 Gen-Probe Incorporated Compositions, methods and kits for determining the presence of trichomonas vaginalis in a test sample
US7700283B2 (en) * 2004-10-21 2010-04-20 New England Biolabs, Inc. Repair of nucleic acids for improved amplification
US7727718B2 (en) * 2005-01-04 2010-06-01 Molecular Research Center, Inc. Reagents for storage and preparation of samples for DNA analysis
US20120109530A1 (en) * 2005-06-16 2012-05-03 Parks Patrick J Method of classifying chemically crosslinked cellular samples using mass spectra
GB0604973D0 (en) * 2006-03-11 2006-04-19 Central Science Lab Csl Of San Purification method and kit
AU2010210666C1 (en) * 2009-02-03 2016-01-28 Ande Corporation Nucleic acid purification

Also Published As

Publication number Publication date
EP2652175A4 (en) 2014-05-21
WO2012082849A1 (en) 2012-06-21
EP2652175A1 (en) 2013-10-23
EP2652175B1 (en) 2017-09-20
JP2014502495A (ja) 2014-02-03
US20120152743A1 (en) 2012-06-21

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