JP6112702B2 - 抗酸化剤 - Google Patents
抗酸化剤 Download PDFInfo
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- JP6112702B2 JP6112702B2 JP2012209965A JP2012209965A JP6112702B2 JP 6112702 B2 JP6112702 B2 JP 6112702B2 JP 2012209965 A JP2012209965 A JP 2012209965A JP 2012209965 A JP2012209965 A JP 2012209965A JP 6112702 B2 JP6112702 B2 JP 6112702B2
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Description
先ず、ユズ果実から種子を分離した。具体的には、十分に熟したユズ果実の表面を水で洗浄した後、下部に切れ込みを入れ、キャタピラ式果実搾汁機(井河鉄工所社製,製品名「果汁搾汁機」)を用いて圧搾することにより、種子を含む果汁を得た。次いで、100メッシュの振動篩を用い、種子を果汁から分離した。得られた種子を、50℃以下の温度で、水分含量が10%以下になるまで乾燥した。
(1) in vitro試験
上記実施例1で得られたユズ種子オイル1容量に対して、1容量のエタノールを加えた。この状態ではユズ種子オイルはエタノールに完全には溶解しなかったので、ピペッティングにより十分に混和した。得られたユズ種子オイル/エタノール混和物を、生理食塩水で5〜56倍に段階的希釈した。また、対照として、上記実施例1で副次的に得られたユズ果汁も同様に希釈した。
実験動物として、雌性のICRマウスを日本SLCより購入し、任意に5匹ずつ6群に分けた。実験開始時には8週齢であった。体内の脂質過酸化を促すために、通常食の代わりに高脂肪食を与えた高脂肪食群を設定した。通常食としては日本クレア社製のマウス・ラット・ハムスター用一般試料CE−2を用いた。高脂肪食としては日本クレア社製のD15001(CE−2+コレステロール2%・コール酸1%・ヤシ油5%)を用いた。餌は供餌器から自由摂取させた。
図1のとおり、蒸留ユズ種子圧搾オイルで顕著な抗酸化作用(SOD様活性)が認められた。また、50%ユズ種子オイル/エタノール混和物のSOD様活性は、ユズ果汁が16.4±5.56(±S.E.)%、未処理ユズ種子圧搾オイルが8.6±3.42%であったのに対し、蒸留ユズ種子圧搾オイルでは54.9±4.76%であった(図2)。各群間のt検定を行ったところ、p値は果汁vs未処理オイルで0.4363、果汁vs蒸留オイルで0.0244、未処理オイルvs蒸留オイルで0.0005と、蒸留ユズ種子圧搾オイルは、ユズ果汁と未処理ユズ種子圧搾オイルに対して有意に優れたSOD様活性を有しているとの結果が得られた。
Claims (3)
- ユズ種子圧搾オイルを含むことを特徴とする抗酸化健康飲食品。
- 請求項1に記載の抗酸化健康飲食品を含むことを特徴とする生活習慣病予防剤。
- 前記生活習慣病が、動脈硬化症、糖尿病、高脂血症、がん又は肝臓機能低下である請求項2に記載の生活習慣病予防剤。
Priority Applications (1)
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