JP6066622B2 - Additives used for meat chicken feed and feed containing the same - Google Patents

Description

  The present invention relates to an additive used for meat chicken feed and a feed containing the same, and more specifically, contains rosemary extract and / or capsicum extract, and prevents oxidation progress of chicken meat and improves storage quality. The present invention relates to an additive used for meat chicken feed useful for food and a feed containing the same.

  Of meat, poultry meat such as chicken, which is said to contain a lot of unsaturated fatty acids, is easily deteriorated in quality and poor in storage stability. The cause is mainly the generation of peroxides (lipid peroxides, etc.) that accompany the oxidation (autooxidation) of unsaturated fatty acids that make up biological membranes. It leads to deterioration of quality and shelf life.

  In order to improve this problem, an antioxidant is orally administered to meat chickens, or an antioxidant is added in the processing of chicken meat. Conventionally, phenolic synthetic antioxidants such as butylhydroxyanisole and dibutylhydroxytoluene have been used as additives for antioxidants in foods, but these are used in terms of safety such as carcinogenicity. Be shunned. Therefore, there is a strong demand for the introduction and conversion of natural antioxidant materials with high safety and powerful effects.

  At present, vitamin E is a typical natural antioxidant and is widely used in food, livestock and poultry. However, it is difficult to say that such natural antioxidants have a sufficient effect on food, livestock and poultry.

On the other hand, conventionally, an essential oil compound and a spice are added to the meat chicken feed (Patent Documents 1 and 2).
However, these essential oil compounds and spices are used for general additives and for preventing the growth of microorganisms in chicken, and are not used for preventing the oxidation of chicken. In addition, it is difficult to analogize the antioxidant effect of chicken by the additive of the present invention, and there is actually no report that has found such an effect.

JP 2009-11320 A JP-A-7-31382

  As mentioned above, in order to prevent the oxidation of chicken meat, antioxidants for oral administration of chicken for meat and antioxidants for direct addition to chicken have been known, but high antioxidant What has an effect and is highly safe is not known, and an antioxidant for chicken that is more excellent in this respect has been desired.

  As a result of intensive studies in view of such problems, the present inventors have made meat chickens ingest a specific extract called rosemary extract and / or capsicum extract, whereby the oxidation of chicken processed from the meat is suppressed. The inventors have found that the storage quality is improved and have completed the present invention.

The present invention is characterized by the following points.
1. An additive used for meat chicken feed, characterized by containing a rosemary extract and / or a capsicum extract.
2. The additive according to 1 above, which contains a rosemary extract and a capsicum extract.
3. Furthermore, the additive of said 1 or 2 characterized by containing carvacrol. 4). 4. The additive according to any one of 1 to 3 above, which is used for preventing oxidation of chicken meat.
5. A chicken feed for meat, comprising the additive according to any one of 1 to 4 above.

  By feeding the meat chicken with the additive containing the rosemary extract and / or capsicum extract of the present invention, the meat obtained from the meat chicken and the processed meat are prevented from progressing in oxidation. It improves the meat quality and fat odor of fat, reduces the fat odor and increases freshness retention, and can improve the storage quality. In particular, the combined use of both the rosemary extract and the capsicum extract significantly increases the antioxidant effect, making it even better as a storage quality improving agent for chicken. Furthermore, the antioxidant effect increases more remarkably by further adding a specific extract or herb essential oil component called carvacrol to the additive containing rosemary extract and / or capsicum extract. Moreover, since the extract and herb essential oil component used for the additive of the present invention are all present in natural products, they are excellent in safety.

  Furthermore, the additive of the present invention can be fed by adding it to a normal meat chicken feed, and since it does not require any special treatment, it is easy to handle at breeding sites and feed blending factories. Have advantages.

Hereinafter, the present invention will be described in more detail.
The raw material of the rosemary extract used in the present invention is an evergreen shrub native to the Mediterranean coastal region classified into the genus Lamiaceae (Rosmarinus). White rosemary (R. officinalis albiflorus), Mallorca pink rosemary (R. Officinalis 'MP') are contained. The rosemary extract used in the present invention is obtained by extracting the above-mentioned rosemary essential oil or leaves and stems with water and / or an organic solvent, and among them, rosemary essential oil is particularly preferable. The method of producing the essential oil is not particularly limited, but rosemary leaves and stems are put into a distillation kettle, and steam is sent into the kettle. The essential oil components are released and vaporized, and rise together with the steam. It can be obtained by cooling the solution to liquid and separating the essential oil and water. The method for producing the water and / or organic solvent extract is not particularly limited, but the rosemary leaves and stems are dried and pulverized, and water, hot water, alcohol solvents such as methanol and ethanol, acetone Extraction with a ketone solvent such as benzene, an aromatic solvent such as benzene, or a mixed solvent thereof. When the essential oil is used, the amount of the rosemary extract to be mixed in the feed is desirably 0.2 to 100 mg per 1 kg of the feed.

  The raw material of Capsicum extract used in the present invention is a plant classified into the genus Capsicum (Solanaceae), such as Capsicum annuum L., Capsicum baccatum, or Capsicum. cardenasii), red pepper (Capsicum frutescens L.), Rokoto (Capsicum pubescens Ruiz & Pav.), and Sinense species (Capsicum chinense Jacq. Heser & Smith). The Capsicum extract used in the present invention is obtained by extracting the fruits of the above-mentioned Capsicum plant with an organic solvent or the like. The extraction method is not particularly limited, but dry and pulverize Capsicum fruit, etc., alcohol solvents such as methanol and ethanol, ether solvents such as ethyl ether, hydrocarbon solvents such as hexane, Examples include extraction with a ketone solvent such as acetone, an aromatic solvent such as benzene, a chlorine solvent such as dichloroethane or chloroform, or a mixed solvent thereof. As a compounding quantity of the capsicum extract in the feed, it is desirable to blend so as to be 0.2 to 200 mg per 1 kg of the feed.

  The carvacrol used in the present invention is also known as isothimol, and can be arbitrarily used either from a natural product or industrially synthesized. As carvacrol derived from natural products, various kinds of Lamiaceae essential oils, in particular origanum oil, or essential oil components derived from himejitsu oil and hiba oil can be used. The amount of carvacrol in the feed is desirably 0.2 to 100 mg per 1 kg of feed.

In the additive of the present invention, when the rosemary extract and the capsicum extract are combined and fed to the chicken for meat, the oxidation of the chicken is significantly suppressed as compared with the case where the extract is fed alone. Moreover, by combining rosemary extract, capsicum extract, and carvacrol, oxidation of chicken is further significantly suppressed as compared with the case where the two components are combined. Therefore, in the present invention, it is desirable to use the extract and herb essential oil component in combination. In the additive of the present invention, particularly preferred combinations of the extract and the herbal essential oil component are as follows.
(1) Rosemary extract, capsicum extract (2) Rosemary extract, carvacrol (3) Capsicum extract, carvacrol (4) Capsicum extract, rosemary extract, carvacrol

  The additive of the present invention can be fed to the chicken for meat as it is, but it is desirable to add the feed to the feed in order for the meat chicken to efficiently consume the spices contained in the additive. . In this case, there is no particular limitation on the feed used as a base when blending the spices, and a feed generally blended with chicken for meat may be used. For example, in addition to the above-mentioned spices added, this feed includes grains such as corn, milo, wheat, barley, buckwheat, rye, brown rice, buckwheat, millet, acne, mackerel, rice bran, corn bran, corn Vegetables such as germs, soybean oil cake, kinako flour, flaxseed oil cake, palm kernel cake, sesame oil cake, sunflower oil cake, rapeseed oil cake, fish meal, fish soluble, meat powder, meat-and-bone meal, blood meal, defatted Animal feed materials such as powdered milk, casein, and dried whey, and oils such as soybean oil, peanut oil, coconut oil, palm oil, tallow, lard, and the like can be included. In the present invention, there is no particular breeding condition other than providing spices as described above, and breeding may be carried out by a normal method.

  The feeding of the additive of the present invention to the chicken for meat can be performed continuously over a period from the start of breeding of the chicken for meat to the time of shipment, so that the chicken can maintain the freshness of the meat quality for a long time. It is preferable from the point. However, even if it is fed over a period of about 3 to 4 weeks or more before shipping meat chickens, it is possible to produce chicken with little reduction in freshness.

EXAMPLES Hereinafter, although an Example demonstrates this invention more concretely, this invention is not limited at all by these Examples.
In the following examples and comparative examples, a rosemary extract 10% adsorbed product of rosemary essential oil manufactured by Takasago International Inc. is used as a rosemary extract, and a capsicum extract manufactured by Takasago International Inc. as a capsicum extract. A 10% carbachlor adsorbed product manufactured by Takasago International Corporation was used as the carvacrol.

[Example 1, Comparative Example 1]
TBA values of chicken meat and peach meat obtained from chickens fed with feed containing the additive of the present invention (Example 1) and chickens fed with normal feed (Comparative Example 1) (thio) By measuring the barbital acid value), the effect of the additive of the present invention on the antioxidant properties of fat contained in chicken was examined.

(Feed formulation)
Rosemary essential oil 10% adsorbed product is added to the basic feed (Comparative Example 1) shown in Table 1 below so that the rosemary essential oil is 0.6 mg / kg, and the feed containing the additive of the present invention ( Example 1) was prepared.

(Raising conditions)
20 chickens for meat (chunky broiler female 21 days old) were fed with the basic feed of Comparative Example 1 for 21 days and 20 chickens for meat (chunky broiler female 21 days old) One feed was fed continuously for 21 days.

(Test method)
Body weight was measured before and after breeding to confirm the growth state.
The TBA value was measured as follows. That is, dismantling was performed on the end of the breeding, and the meat and peach meat were collected and stored for 6 days under chilled conditions (5 ° C.). Homogenate was carried out by adding EDTA (ethylenediaminetetraacetic acid) .2Na 0.15 g, water 50 ml, and 15% BHA (butylhydroxyanisole) -ethanol solution 1.0 ml to 5 g of preserved meat or peach meat. Further, 50 ml of water, 2.5 ml of 4 mol / L acetic acid solution, 50 g of sodium chloride and an antifoaming agent were added and steam distillation was performed. 5 ml was taken from 50 ml of the distillate, added with TBA reagent (5 ml of a mixture of 0.02 mol / L TBA and acetic acid), and heated in a boiling oil bath at 100 ° C. for 30 minutes. After allowing to cool, the butanol layer was separated by centrifugation. The TBA value was measured by measuring the absorbance at 532 nm of the separated butanol layer.

(Test results)
The test results relating to growth performance are shown in Table 2 below, and the measurement results relating to TBA values are shown in Tables 3 and 4 below.

[Example 2, Comparative Example 2]
AV (acid value) value of intraperitoneal fat obtained from chickens fed with feed containing the additive of the present invention (Example 2) and chickens fed with normal feed (Comparative Example 2) The effect of the additive of the present invention on the antioxidant properties of fat contained in chicken was examined.

(Feed formulation)
Rosemary essential oil 10% adsorbed product is added to the basic feed (Comparative Example 2) shown in Table 5 below so that the rosemary essential oil is 0.8 mg / kg, and the feed containing the additive of the present invention ( Example 2) was prepared.

(Raising conditions)
20 chickens for meat (chunky broiler male 13 days old) were fed with the basic feed of Comparative Example 2 for 22 days and 20 chickens for meat (chunky broiler male 13 days old) Two feeds were fed continuously for 22 days.

(Test method)
Body weight was measured before and after breeding to confirm the growth state.
The AV value was measured as follows. That is, the animals were disassembled on the end of the breeding period, and intraperitoneal fat was collected and stored for 3 days under conditions of 40 ° C. and humidity 75%. 10 g of oil was extracted from 20 g of intraperitoneal fat after storage. Using 3 g of the oil and fat, 50 ml of diethyl ether (special grade) and 50 ml of ethanol (special grade 99.5%) were added and stirred with a glass rod to dissolve the fat and oil. Add 2 to 3 drops of indicator (phenol phthalene solution), stir lightly with a crow bar, and drop potassium hydroxide solution until it reaches the top color (pink color does not disappear). Was calculated.

(Test results)
The test results relating to growth performance are shown in Table 6 below, and the measurement results relating to AV values are shown in Table 7 below.

[Example 3, Comparative Example 3]
The AV value of the intraperitoneal fat obtained from a chicken fed with a feed containing the additive of the present invention (Example 3) and a chicken fed with a normal feed (Comparative Example 3) By measuring the TBA value, the effect of the additive of the present invention on the antioxidant properties of fat contained in chicken was examined.

(Feed formulation)
Rosemary essential oil 10% adsorbed product is added to the basic feed (Comparative Example 3) shown in Table 8 below so that the rosemary essential oil is 1.32 mg / kg, and the feed containing the additive of the present invention ( Example 3) was prepared.

(Raising conditions)
20 chickens for meat (chunky broiler female 21 days old) were fed with the basic feed of Comparative Example 3 for 21 days and 20 chickens for meat (chunky broiler female 21 days old) 3 feeds were fed ad libitum for 21 days.

(Test method)
Body weight was measured before and after breeding to confirm the growth state. In addition, the AV value was measured as described in Example 2 and Comparative Example 2, and the TBA value was measured using 5 g of a skin of the skin after storage for 10 days under chilled conditions (5 ° C.). Others were carried out as described in Example 1 and Comparative Example 1.

(Test results)
The test results on growth performance are shown in Table 9 below. Further, measurement results relating to AV values are shown in Table 10 below, and measurement results relating to TBA values are shown in Table 11 below.

[Example 4, Comparative Example 4]
Measurement of TBA values of chicken and peach meat obtained from chickens fed with feed containing the additive of the present invention (Example 4) and chickens fed with normal feed (Comparative Example 4) Thus, the effect of the additive of the present invention on the antioxidant properties of fat contained in chicken was examined.

(Feed formulation)
The feed containing the additive of the present invention (Example 4) was added to the basic feed (Comparative Example 4) having the blending ratio shown in Table 12 below so that the 10% Capsicum extract was adsorbed to 100 mg / kg. ) Was prepared.

(Raising conditions)
20 chickens for meat (chunky broiler female 21 days old) were fed with the basic feed of Comparative Example 4 for 21 days and 20 chickens for meat (chunky broiler female 21 days old) 4 feeds were fed ad libitum for 21 days.

(Test method)
Body weight was measured before and after breeding to confirm the growth state. The TBA value was measured as described in Example 1 and Comparative Example 1.

(Test results)
The test results relating to growth performance are shown in Table 13 below, and the measurement results relating to TBA values are shown in Tables 14 and 15 below.

[Example 5-1, Example 5-2, Comparative Example 5]
Intraperitoneally obtained from chickens fed with feed containing the additive of the present invention (Example 5-1 and Example 5-2) and chickens fed with normal feed (Comparative Example 5) By measuring the AV value of fat, the effect of the additive of the present invention on the antioxidant properties of fat contained in chicken was examined.

(Feed formulation)
0.2 mg / kg (Example 5-1) or 100 mg / kg (Example 5-) as a rosemary essential oil with 10% rosemary essential oil adsorbed product in the basic feed (Comparative Example 5) shown in Table 16 below. 2) was added to prepare a feed containing the additive of the present invention.

(Raising conditions)
42 chickens for meat (chunky broiler female 25-day-old) divided into 3 groups of 14 each, and the basic feed of Comparative Example 5, the feed of Example 5-1 or the feed of Example 5-2 for 20 days Feeded constantly.

(Test method)
Body weight was measured before and after breeding to confirm the growth state. The AV value was measured as described in Example 2 and Comparative Example 2, except that intraperitoneal fat was stored for 10 days under conditions of 40 ° C. and 75% humidity.

(Test results)
The test results relating to growth performance are shown in Table 17 below, and the measurement results relating to AV values are shown in Table 18 below.

[Example 6-1, Example 6-2, Comparative Example 6]
Intraperitoneally obtained from chickens fed with feed containing the additive of the present invention (Example 6-1 and Example 6-2) and chickens fed with normal feed (Comparative Example 6) The effect of the additive of the present invention on the antioxidant properties of fat contained in chicken was examined by measuring the AV value of fat and the TBA value of the skin of the skin.

(Feed formulation)
Capsicum extract 10% adsorbed product was added to capsicum extract 0.2 mg / kg (Example 6-1) or 200 mg / kg (Example 6-2) in the basic feed (Comparative Example 6) shown in Table 19 below. The feed containing the additive of the present invention was prepared.

(Raising conditions)
Forty-two chickens for meat (chunky broiler female 25-day-old) divided into three groups of 14 chickens, and the basic feed of Comparative Example 6, the feed of Example 6-1 or the feed of Example 6-2 for 20 days Feeded constantly.

(Test method)
Body weight was measured before and after breeding to confirm the growth state. The AV value was measured as described in Example 2 and Comparative Example 2 except that the fat in the abdominal cavity was stored for 10 days under the condition of 20 ° C. The TBA value was measured using a sample. As described in Example 1 and Comparative Example 1, except that 5 g of rind skin after storage for 10 days under chilled conditions (5 ° C.) was used.

(Test results)
The test results on growth performance are shown in Table 20 below. In addition, measurement results relating to AV values are shown in Table 21 below, and measurement results relating to TBA values are shown in Table 22 below.

[Example 7-1, Example 7-2, Example 7-3, Comparative Example 7]
Chickens fed with feed containing the additive of the present invention (Example 7-1, Example 7-2, Example 7-3) and chickens fed with normal feed (Comparative Example 7) The effect of the additive of the present invention on the antioxidant properties of fat contained in chicken was examined by measuring the TBA value of the rice skin obtained from (1).

(Feed formulation)
Addition of the present invention to the basic feed (Comparative Example 7) shown in Table 23 below with a 10% rosemary essential oil adsorbent and / or 10% capsicum extract adsorbed so as to have the following concentrations: A feed containing the preparation was prepared.
Example 7-1: Rosemary essential oil 2.0 mg / kg
Example 7-2: Capsicum extract 2.0 mg / kg
Example 7-3: Rosemary essential oil 2.0 mg / kg, Capsicum extract 2.0 mg / kg

(Raising conditions)
Forty meat chickens (chunky broiler male 30 days old) were divided into four groups of 10 chickens each, and the basic feed of Comparative Example 7 or the feeds of Examples 7-1 to 7-3 were fed continuously for 21 days.

(Test method)
Body weight was measured before and after breeding to confirm the growth state. In addition, the TBA value was measured as described in Example 1 and Comparative Example 1 except that 5 g of the skin of the skin after storage for 2 days under chilled conditions (5 ° C.) was used as a sample.

(Test results)
The test results on growth performance are shown in Table 24 below. In addition, Table 25 below shows the measurement results regarding the TBA value.

[Example 8-1, Example 8-2, Example 8-3, Comparative Example 8]
Chickens fed with feed containing the additive of the present invention (Example 8-1, Example 8-2, Example 8-3) and chickens fed with normal feed (Comparative Example 8) The effect of the additive of the present invention on the antioxidant properties of fat contained in chicken meat was examined by measuring the AV value of intraperitoneal fat obtained from the above and the TBA value of the skin.

(Feed formulation)
An additive of the present invention is added to a basic feed (Comparative Example 8) having a blending ratio shown in Table 26 below by adding a rosemary essential oil 10% adsorbed product and / or carvacrol 10% adsorbed product to the following concentrations: A feed containing was prepared.
Example 8-1: Rosemary essential oil 2.0 mg / kg
Example 8-2: Carvacrol 2.0 mg / kg
Example 8-3: Rosemary essential oil 2.0 mg / kg, carvacrol 2.0 mg / kg

(Raising conditions)
Forty meat chickens (chunky broiler male 30 days old) were divided into four groups of 10 each, and the basic feed of Comparative Example 8 or the feeds of Examples 8-1 to 8-3 were fed continuously for 21 days.

(Test method)
Body weight was measured before and after breeding to confirm the growth state. In addition, the AV value was measured as described in Example 2 and Comparative Example 2 except that the intraperitoneal fat was stored for 10 days under the conditions of 40 ° C. and 75% humidity. The measurement was carried out as described in Example 1 and Comparative Example 1, except that 5 g of the skin of the skin after storage for 2 days under chilled conditions (5 ° C.) was used as a sample.

(Test results)
The test results on growth performance are shown in Table 27 below. Further, measurement results relating to AV values are shown in Table 28 below, and measurement results relating to TBA values are shown in Table 29 below.

[Example 9, Comparative Example 9]
Measuring AV values of intraperitoneal fat obtained from chickens fed with feed containing the additive of the present invention (Example 9) and chickens fed with normal feed (Comparative Example 9) Thus, the influence of the additive of the present invention on the antioxidant properties of fat contained in chicken meat was examined.

(Feed formulation)
To the basic feed (Comparative Example 9) shown in Table 30 below, a rosemary essential oil 10% adsorbed product, a capsicum extract 10% adsorbed product and a carvacrol 10% adsorbed product are added to the following concentrations. A feed containing the additive of the present invention was prepared.
Example 9: Rosemary essential oil 1.2 mg / kg, Capsicum extract 2.0 mg / kg, Carvacrol 2.0 mg / kg

(Raising conditions)
14 chickens for chicken (chunky broiler female 25 days old) were fed with the basic feed of Comparative Example 9 for 20 days and 14 chickens for meat (chunky broiler female 25 days old) Nine feeds were fed continuously for 20 days.

(Test method)
Body weight was measured before and after breeding to confirm the growth state. The AV value was measured as described in Example 2 and Comparative Example 2 except that the fat in the abdominal cavity was stored for 10 days at 20 ° C.

(Test results)
The test results relating to growth performance are shown in Table 31 below, and the measurement results relating to AV values are shown in Table 32 below.

From the above analysis results, the additive of the present invention can suppress the progress of oxidation of the meat obtained from the meat chicken and the processed meat without adversely affecting the growth of the meat chicken. It was confirmed that storage quality could be improved.
In particular, meat obtained by feeding meat chickens with an additive combining two or three of the three specific components selected from rosemary extract, capsicum extract and carvacrol, and processed meat thereof It has been clarified that the degree of inhibition of the progress of oxidization of the water increases dramatically.

Claims (4)

An additive used for meat chicken feed, comprising a rosemary extract and carvacrol . The additive according to claim 1, further comprising a capsicum extract .   The additive according to claim 1 or 2, wherein the additive is used for preventing oxidation of chicken meat.   Meat chicken feed comprising the additive according to any one of claims 1 to 3.