JP6056040B1 - Eustoma production method - Google Patents

Abstract

[PROBLEMS] To provide a method for producing an eastoma that can prevent rosette formation without requiring a large refrigeration facility, and has less problems such as abnormal flowering and reduced germination rate, and can further reduce the labor burden for management. To do. [Solution] A seed having a particle size that can be separated from the seed, a carrier having water retention, a mixing step of mixing water, and the mixture obtained in the mixing step is prevented from being rosette. A seed cooling step of storing at a temperature exhibiting an effect, and seeds obtained by the seed cooling step are sown, raised, planted, cultivated and flowered to produce a flower. [Selection] Figure 1

Description

  The present invention relates to a method for cultivating and producing an eustoma. More specifically, the present invention relates to a method for producing an eastoma that can prevent rosettes after planting even in summer, and can cultivate eastoma at a low production cost.

  Eustoma is also said to be Eustoma and has a variety of varieties and flower colors, and is a popular Western flower for ceremonial occasions and arrangements. Eustoma is a perennial plant that is sown in the autumn and blooms in the following spring under natural conditions, but because of the high demand in the winter, especially during the spring season, it is forcibly cultivated from autumn to winter. Cultivation) is widely performed.

  However, seedlings are grown in summer in autumn / winter cultivation, but rosetting, early extraction, etc. are likely to occur in seedlings in the high temperature period. When roseting or brewing occurs, the value of the product as a flower is greatly reduced, which causes production problems. Particularly in recent years, the problem of rosetting has become prominent due to global warming and the like. Therefore, various methods for preventing the rosette formation of the elastomer have been proposed.

  In Patent Document 1, the time of sowing is advanced to the time (pre-summer) when seedlings that do not become rosette are obtained (before summer), and the grown seedlings are set to a range of more than 0 ° C and less than 8 ° C in an unroseted state. A forcible cultivation method for eustoma has been disclosed in which a low-temperature treatment is applied, a fixed planting time is reached, and the plant grows by planting and blooms by the winter or the first spring of the following year. By this method, rosetting after planting can be reduced.

  Also known is a method in which a rosette is intentionally made immediately after sowing by high temperature treatment or the like, and then subjected to low temperature treatment to release the rosette (breaking the rosette). For example, seedlings are seeded around June and rosettes are obtained around July, and the rosettes are kept at a low temperature of 10-12 ° C. until around September to break down the rosettes. There is a known method of planting and growing in Japan to reach flowering in winter. However, in this method, when seedlings that are not completely rosette are mixed, there is a problem that extraction of moss starts during processing and the quality of the seedlings deteriorates.

  In addition, the above method requires a large amount of refrigeration equipment for low temperature treatment of the grown seedlings, and irrigation is always required during the period of keeping the rosette seedlings at a low temperature. There is also a problem that the labor burden for management increases.

  Therefore, a method for preventing rosetting by cooling seeds instead of growing seedlings (seed cooling treatment) has been put into practical use. For example, after seeding a coated seed on a plug tray, the plug tray is put in a refrigerator and seed cooling treatment is performed at about 10 ° C. for 30 days (standard). After removing the seed-cooled plug tray from the refrigerator, it is bred for about 40 days at night, planted in the field, and then planted for about 70-90 days, and then flowered, but this method can prevent rosette formation.

JP-A-9-191761

  However, even in this method, the seed cooling process is performed in a state in which the coated seeds are sown on the plug tray, so that a large refrigerator is required. In addition, since it is necessary to manage moisture during seed cooling and to prevent the occurrence of illness, the labor burden has been insufficiently reduced. Therefore, it has been desired to develop a method that can prevent the formation of rosettes with smaller equipment and can further reduce the labor burden for management.

  The present invention provides a method for cultivating yeast that can prevent rosette formation without requiring a large refrigeration facility, is easy to manage without problems such as abnormal flowering and a decrease in germination rate, and can reduce labor burden. The issue is to provide.

  As a result of the study, the present inventors have been able to prevent rosette formation even when growing seedlings in the high temperature summer season by mixing seeds with a carrier having water retention and water and then cooling the seeds. The present inventors have found that a large refrigeration facility is not required and that the seed cooling is easy to manage, and that there are few problems such as abnormal flowering and a decrease in germination rate.

The present invention
Eustoma seeds, a carrier comprising water particles having a particle size that can be separated from the seeds, a mixing step of mixing water, and a mixture obtained in the mixing step at a temperature exhibiting a rosette-preventing effect. A seed cooling step for storing, wherein the seed obtained by the seed cooling step is seeded, raised, planted, cultivated and cultivated to flower.

The present invention is also a preferred embodiment (hereinafter referred to as “first embodiment”) of the production method of the above-mentioned elastomer,
After the seed cooling step, before the sowing, the method further comprises the step of separating the seed and the carrier, and coating the separated seed with an inorganic substance to obtain a coated seed. .

  The present invention further provides a preferred embodiment of the above-mentioned method for producing an eusta (hereinafter referred to as “second embodiment”), wherein the seed of the eusuma is a coated seed. To do.

  Furthermore, according to the present invention, as a preferred aspect of the above-described method for producing an euste, the following method for producing an euste is provided.

  The above-mentioned method for producing an elastomer according to the present invention, wherein the carrier comprises particles having a particle size smaller than that of the seed.

  The method for producing an elastomer according to the present invention, wherein the carrier is diatomaceous earth or alumina.

  The method for producing an elastomer according to the present invention, wherein the mixture contains a bactericide.

  The volume of seeds is small, and in the case of bare seeds, about 25,000-35,000 grains / g is common. The volume of the coated seed varies depending on the particle diameter, but is about 500 to 1000 grains / g when the particle diameter is about 1.5 mmφ. Since the seed volume is small, the volume of the mixture of seed, carrier and water is also small. In the method of the present invention, seed cooling is performed on this small volume mixture, so that seed cooling can be performed in a small refrigerator, for example, an inexpensive refrigerator commercially available for home use. In the conventional method, the seed cooling is performed by seeding the coated seeds on the plug tray and then putting the plug tray in the refrigerator, so a large refrigerator is necessary. However, in the method of the present invention, the plug tray is inserted. There is no need to install a large refrigerator.

  In addition, according to the method of the present invention, moisture management and the like in seed cooling are easy, so that the labor burden can be reduced. There are no problems such as abnormal flowering time, seedling height, flower shape, or germination rate.

That is,
Rosette can be prevented even when raising seedlings in summer high temperature season (autumn cultivation)
Since it does not require a large refrigeration facility and the labor burden for management such as seed cooling can be reduced, production costs can be greatly reduced.
There are few problems such as abnormal flowering and lower germination rate,
And an excellent production method of eusumas.

6 is a photograph showing the results of Example 3. 6 is a photograph showing the results of Comparative Example 2. 6 is a photograph showing the results of Example 4. 6 is a photograph showing the results of Comparative Example 3.

  Next, the form for implementing this invention is demonstrated concretely. In addition, this invention is not limited to the following form and the below-mentioned Example, All the changes in the range equivalent to a claim and a claim are included.

The first aspect of the present invention is one of the preferred embodiments of the method for producing the above-mentioned eusta,
Eustoma seed, a carrier having a particle size that can be separated from the seed, and a water-retaining carrier, a mixing step of mixing water, and seed cooling for storing the resulting mixture at a temperature exhibiting a rosette-preventing effect Separating the seed and the carrier after the seed cooling step,
The separated seed is coated with an inorganic substance to obtain a coated seed,
It is a method for producing an eastoma characterized in that the obtained coated seeds are sown, planted, planted, cultivated and flowered.

2nd aspect of this invention is one of the preferable aspects of the production method of the said elastomer,
Eustoma seeds, a carrier comprising water particles having a particle size that can be separated from the seeds, and a mixing step of mixing water, and seeds for storing the resulting mixture at a temperature exhibiting a rosette-preventing effect A cooling step, wherein the seed of the eusumer is a coat seed,
After the seed cooling step, the seed and the carrier are separated,
A method for producing an eastoma, characterized in that the separated seeds are sown, planted, planted, cultivated and flowered.

  Hereinafter, the seed, carrier, moisture, and other components used as necessary, and each process of the present invention used in the present invention will be described.

1. Eustoma Seeds Unless otherwise specified, “Eustoma Seeds” includes both bare seeds and coated seeds. Here, the naked seed means a seed that is not coated. Pre-treated seeds such as priming, and water-soluble polymers containing chemicals such as bactericides such as PVA (polyvinyl alcohol), MC (methyl cellulose), CMC (carboxymethyl cellulose), and HEC (hydroxyethyl cellulose) are coated. Seeds are also included in bare seeds.

  The coated seed means a seed coated with a coating material mainly composed of an inorganic substance, and is also called a pellet seed. The coating material mainly composed of an inorganic substance contains the inorganic substance as a component having the maximum content, but may contain other components such as a binder as long as the gist of the present invention is not impaired. In the first embodiment, bare seeds are usually used as the seeds of the yeast. In the second embodiment, coat seeds are used as the seeds of the eusumer.

2. Carrier (carrier particle size)
As the carrier, a powdery or granular material composed of particles having a particle size that can be separated by sieving from a mixture with seeds of eustoma can be used. After the seed cooling step, the carrier is separated from the seeds of the eastoma by sieving. In order to sufficiently separate the carrier, the particles constituting the carrier are the lower limit of the seed particle size distribution (minimum seed diameter). It is desirable that the particles are made of particles having a smaller particle size, or particles having a particle size larger than the upper limit (the maximum diameter of the seed) of the seed particle size distribution.

  That is, the term “particles having a particle size that can be separated from the mixture by sieving” means particles having a particle size smaller than the minimum diameter of the seeds of eustasis, and / or particles having a particle size larger than the maximum diameter of the seeds. It means particles consisting only of particles having particles, particles having a particle diameter smaller than the minimum diameter of the seed of the European, and / or particles mainly composed of particles having a particle diameter larger than the maximum diameter of the seed. By using a carrier composed of particles of such a particle size that does not cause problems such as aggregation, it is possible to sufficiently separate the seeds from the seeds by sieving (excellent extractability is obtained). The carrier is hardly mixed into the seeds after the division.

  Note that “mainly” substantially consists of particles having a particle diameter smaller than the minimum diameter of the seed or / and particles having a particle diameter larger than the maximum diameter of the seed. It means that other particles may be contained within a range that does not impair. For example, in the case of the first aspect, since the seeds after separation by sieving are coated, seed coating may be inhibited if there is a large amount of carrier contained in the seeds of eastern rice after sieving. As long as such a problem does not occur, other particles may be included.

  Among the above carriers, the carrier mainly composed of particles having a particle size smaller than the seed of the eustasis, that is, a carrier composed only of particles having a particle size smaller than the minimum diameter of the seed, or particles having a particle size smaller than the minimum diameter of the seed. The carrier to be used is preferable because it has advantages such that the amount of the carrier used may be relatively small, the amount of water in the mixture can be reduced, a reduction in germination rate can be suppressed, and the dispersibility in the mixture is good.

  Usually, the bare seed has a minimum diameter of about 250 μm, and therefore, when the seed of the oustoma is a bare seed (first aspect), the carrier is preferably composed mainly of particles having a particle diameter of less than 250 μm. If particles with a particle size of 250 μm or more are included, the carrier may be mixed into the seed after sieving, and it may be difficult to perform seed coating or the like, so the number of particles with a particle size of 250 μm or more should be as small as possible. Is desirable and preferably not included. In order to more reliably separate the seed and the carrier by sieving, when the seed of the elastomer is a bare seed, the particle size of the carrier is more preferably 100 μm or less, and even more preferably 50 μm or less.

  Even when the seed of the yeast is a coated seed (second embodiment), a carrier mainly composed of particles having a particle diameter smaller than the minimum diameter of the coated seed is preferable. Coated seeds have various particle sizes, but generally have a particle size in the range of 1 to 2 mmφ. In the case of coated seeds having a particle diameter in the range of 1 to 2 mmφ, a carrier mainly composed of particles having a particle diameter of less than 1 mmφ can be used. However, if the particle diameter of the carrier is too large, water retention and rosette prevention effects are obtained. Since it decreases, a carrier having a particle size of 100 μm or less is more preferred, and more preferably 50 μm or less.

  When using a carrier composed of particles having a particle size smaller than the minimum diameter of the seed, the minimum particle size of the carrier particles is not particularly limited, but if the particle size is small, powdering tends to occur and workability may be reduced. . Also, depending on the type of carrier, when small particles and water are mixed, they may agglomerate into a dumpling, which makes it difficult to screen. Therefore, a range in which such a problem does not occur is desirable. Usually, particles having a particle size of 1 μm or more are more preferable, and particles having a particle size of 5 μm or more are particularly preferable. That is, the particle diameter of the carrier particles is more preferably in the range of 1 μm to 100 μm, and particularly preferably in the range of 5 μm to 50 μm, regardless of whether the seeds of the eusumer are bare seeds or coated seeds.

(Water retention)
The carrier is mixed with moisture and absorbs water, and acts to impart absorbed moisture to the seeds. Therefore, the carrier has water absorption and can hold free water in and around the seeds during the seed cooling process. A property that always gives moderate moisture to the seed, that is, a material having water retention is used. Therefore, for example, inorganic powders and granules (inorganic carrier) having the property of absorbing water are used.

  The carrier is insoluble in water, but the carrier particles are not to prevent easy separation from the seed due to adhesion between the particles of the carrier due to moisture or the like and granulation (secondary aggregation). Those having no tackiness are suitable.

(Mixing amount)
The amount of the carrier used (mixed amount) is 1 to 500 times the mass of the seed in both the case of the bare seed and the case of the coated seed. More preferably, it is 10 to 100 times the mass of the seed. Here, the seed mass in the case of a coated seed means the mass of the seed before the coating contained in the coated seed. When seed cooling is performed by adding moisture to the seed without using a carrier, the germination rate is extremely reduced. On the other hand, if the amount of the carrier used is too small, the effect of seed cooling cannot be obtained and the formation of rosette is likely to occur. In addition, workability in mixing decreases. On the other hand, when the amount of the carrier used is too much compared to the amount of seed, the workability in mixing is lowered and it is difficult to disperse. Moreover, the workability | operativity at the time of isolate | separating from a seed by sieving falls also and it becomes difficult to take out.

(Example of carrier)
The carrier is composed of particles having a particle size that can be separated from the seeds by sieving, has water retention, can disperse seeds when mixed with seeds, and is sieved after mixing. Therefore, it is required that separation from seeds is easy (separation is not hindered by adhesiveness or the like). As long as these requirements are satisfied, the type of the carrier is not particularly limited, but an inorganic carrier is preferable in that firing described below can be performed.

  Examples of the inorganic carrier include diatomaceous earth, alumina, silica gel, clay and the like. Among them, diatomaceous earth and alumina have excellent dispersibility (easy to disperse when seeds are mixed with the carrier) and excellent take-out properties (no secondary aggregation, etc., and separation of the carrier and seeds by sieving) Is particularly preferable. The carrier may be composed of one kind of carrier, but may be a mixture of two or more kinds of carriers.

(Sintering and sterilization of carrier)
In the case of an inorganic carrier, it is preferable to sterilize the carrier by baking. By sterilizing by baking or the like, generation of mold during seed cooling can be suppressed. When baked, the bulk density of the carrier increases, the water retention increases, the amount of oxygen contained in the carrier increases, and contributes to the improvement of germination rate.

3. Water The seed and carrier mixture further contains water. Examples of the water include tap water, exchange water, distilled water, and the like, but water that dissolves a very small amount of impurities and the like is also used unless the water detracts from the spirit of the present invention (for example, water containing various germs). There is no particular limitation.
The moisture content is preferably 0.01 mass times or more of the carrier in the case of bare seeds and coated seeds. More preferably, it is in the range of 0.05 to 1.0 times the mass of the carrier. When the water content is too small, it is difficult to obtain the seed cooling effect, that is, the effect of preventing rosette formation. On the other hand, if the water content is too high, it becomes difficult to separate the seed and the carrier after the seed cooling step. Moreover, when there is too much content of water, a germination rate falls.

4). Other ingredients, fungicides, etc. The mixture of seeds, carrier and water is further separated in the range not impairing the gist of the invention, i.e., water retention to give the seeds appropriate moisture, etc. However, other components can be used in combination as long as they do not cause problems such as abnormal flowering. For example, one or more selected from bactericides, nutrients and hormones can be used in combination. In particular, it is preferable to use a bactericidal agent in combination because it can suppress the occurrence of mold on the seeds and improve the germination rate.

5). Mixing step Seed, carrier, and water can be mixed using known methods, means, devices, and the like used in the same mixing step. As the conditions for the mixing step, the same conditions as known methods can be employed. In the case of naked seeds, the order of mixing is not particularly limited, but it is often preferable to mix a seed and a carrier and then add a predetermined amount of water and further mix. In the case of coated seeds, the seed coat may be broken when water is added to the seeds. Therefore, after adding water to the carrier and mixing, the order of adding and mixing seeds is usually preferred. When using small containers such as small plastic sealed containers for home use (so-called tappaware) or small plastic bags, add a predetermined amount of seeds, carrier and water to the small container, and shake the container. In this case, the order of addition is not particularly limited.

6). Seed cooling step The mixture of seeds, carriers, water, and the like prepared as described above is stored at a temperature that exhibits a rosette-preventing effect, and seed cooling is performed. When the temperature of seed cooling is too high or too low than the temperature showing the effect of preventing rosette, sufficient effect of preventing rosette cannot be obtained. The temperature showing the effect of preventing rosette is in the range of 1 to 15 ° C. for most varieties, and is usually preferably 7 ° C. or higher and 12 ° C. or lower, particularly preferably 9 ° C. or higher and 11 ° C. or lower. . However, the preferred range varies depending on the variety of seeds, the type of carrier, etc., so it is preferable to conduct a small-scale preliminary experiment in advance to ascertain the optimum seed cooling temperature.

  The preferable range of the seed cooling period (low temperature storage period) varies depending on the variety of the eastoma, but is usually preferably 30 to 50 days, more preferably 35 to 45 days. In order to optimize the amount of water to be mixed, the seed cooling period, and the temperature, it is preferable to conduct a small-scale preliminary experiment in advance and select the above conditions based on the results.

  Seed cooling can be performed by storing the mixture in a refrigerator. Specifically, for example, the mixture can be put in a small container such as a small plastic sealed container for home use or a plastic bag such as polyethylene, and the container can be stored in a refrigerator. The small container is preferably made of a material that passes oxygen and does not allow moisture to pass. Since the mixture can be subdivided into small volumes, an inexpensive household refrigerator can be used as the refrigerator, and the production cost can be greatly reduced as compared with the conventional method.

  In order to compensate for the decrease in moisture due to evaporation, it is preferable to appropriately add moisture to the mixture during seed cooling. Moreover, in order to prevent uneven distribution of moisture and the like due to the position in the mixture, it is preferable to mix the mixture (stirring, shaking the container, etc.) at appropriate intervals during seed cooling. However, compared to the management required for moisture management during seed cooling and prevention of disease occurrence in the method described in Patent Document 1, in the present invention, the frequency of water addition and agitation can be suppressed. Can greatly reduce the labor burden for management.

7). Separation process After the seed cooling process, the seed and the carrier are separated. Separation can be performed using a sieve. By removing moisture from the separated carrier, for example, in the case of an inorganic carrier, it can be used again (for example, the next year) in the method of the present invention by firing.

8). Seed coat (1) In the case of the first embodiment, the separated seed is then coated with an inorganic substance (seed coat) to produce a coated seed. Seed coating methods, conditions, materials and equipment used, etc. are the same as in the case of conventional seed coating of seeds. For example, coated seeds can be produced by the methods described in JP-A-58-18748 and JP-A-61-500101.
(2) In the case of the second aspect, since the mixing step is performed using the coated seed, the seed coating after the separation step is not normally performed. (It is also possible to interpret that the bare seed has a seed coating step with an inorganic substance or the like before the mixing step.) According to the second aspect, since the seed coating after the separation step is unnecessary, the producer of the eastoma Need not be provided with equipment for seed coats, and does not require techniques for seed coats. As the coated seed, a commercially available inexpensive coated seed can be used, so that the production cost of the elastomer can be reduced.

9. Sowing, raising seedlings, fixed planting, cultivation After the above-mentioned steps, the work of sowing the coated seeds is performed. In the case of the second aspect, the seed coating step can be omitted, and the sowing operation as the next step can be started. After being sown, seedlings, planting, cultivating and flowering produce an eastoma. Seeding, raising seedlings, fixed planting, cultivation methods, conditions, equipment used, and the like are the same as in the case of cultivation of publicly known eastomas. In warm regions, cold seedlings may be required at night.

Implementation 1 (carrier screening)
Eustoma bare seeds (trade name: Summer of Okhotsk, average particle size of about 300 μm) in a 100 ml plastic sealed container for home use, each of the carriers listed in Table 1 (after sieving with a sieve having a mesh size of 250 μm) 20 g was added, and an aqueous solution (moisture) containing 0.1% by mass benrate T (bactericidal agent) was added to the amount (% by mass with respect to the carrier) shown in the column of the amount of moisture added in Table 2, and then the sealed container was Hold in hand and shake vigorously for 1 minute.

  After shaking, the mixed state was judged visually, and the degree of dispersion (dispersibility) when seeds were mixed with the carrier was visually evaluated according to the following criteria. The results are shown in Table 2.

[Dispersibility]
(Double-circle): The sticking part is not observed at all.
○: A little sticky part is seen.
(Triangle | delta): There are many sticking parts.
X: Adhesive (secondary aggregation) and not mixed.

The mixture after shaking was sieved with a sieve having a mesh size of 250 μm, and seeds were taken out. The ease of removal (removability) at this time was determined according to the following criteria, and the results are shown in Table 2.
[Removability]
Good: The seed and the carrier can be easily separated, and no carrier is observed in the seed.
Somewhat: Seeds and carriers are separated, but it is observed that the carriers are contained in the seeds.
No: Seed and carrier do not stick and separate.

  The results in Table 2 indicate that diatomaceous earth and alumina are preferable as the carrier, and diatomaceous earth is particularly preferable from the viewpoints of dispersibility and extractability.

Example 2
In a commercially available 100 ml polyethylene bag, 1 g of bare seed of Eustoma (trade name: Summer of Okhotsk, average particle size of about 300 μm, germination rate of 100%), diatomaceous earth or alumina listed in Table 1 (with mesh of 250 μm) 2 g, 4 g or 6 g of an aqueous solution (moisture) containing 0.1% by mass Benrate T (bactericidal agent) (the amount of water added is 10% by mass and 20% by mass, respectively) with respect to the carrier. % Or 30% by weight), the polyethylene bag was closed and shaken vigorously for 1 minute.

  The shaken polyethylene bag was placed in a household 350 L refrigerator and stored (seed cooling) at 10 ° C. (humidity 80%) for 30 days. During storage, the polyethylene bag was opened and then closed and shaken every 7 days. After storage, the mixture was taken out from the polyethylene bag and sieved with a sieve having a mesh size of 250 μm to separate the seeds.

  The separated seeds were coated with an inorganic substance by a known method (the method described in JP-A No. 58-18748) to produce coated seeds having an average particle diameter of 1.5 mmφ. The obtained coated seeds were dried with hot air at 30 ° C. for 20 hours. After sowing on the plug tray, the seedlings were grown for 21 days to confirm the germination state, and the germination rate (the ratio of germination in the seeds sowed) was examined. The results are shown in Table 3.

Comparative Example 1
Eustoma bare seeds (trade name: Summer of Okhotsk, average particle size of about 300 μm, germination rate of 100%), 1 g, and 0, 1% by mass of Benrate T (bactericidal agent) in a commercially available 20 ml polyethylene bag Coated seeds were prepared in the same manner as described above except that 0.5 g, 0.75 g, and 1.0 g of an aqueous solution containing water were added, respectively, with shaking, seed cooling, sieving, and seed coating. The obtained coated seeds were dried with hot air at 30 ° C. for 20 hours. After seeding on the plug tray, seedlings were grown for 21 days to confirm the germination state and the germination rate was examined. The results are shown in Table 3.

  From the results of Example 2 and Comparative Example 1, when diatomaceous earth and alumina are used as a carrier and water is added to cool the seed, a high germination rate can be obtained. It has been shown that when seeds are cooled with water added, the germination rate is extremely reduced.

Example 3
Into a commercially available 100 ml polyethylene bag, 1 g of bare bare seeds (trade name: Summer of Okhotsk, average particle size of about 300 μm, germination rate of 100%), 20 g of diatomaceous earth listed in Table 1 were added, and further 0.1 4 g of an aqueous solution (moisture) containing mass% benrate T (disinfectant) was added (the amount of water added to the carrier was 20 mass%), and then the polyethylene bag was closed and vigorously shaken for 1 minute.

  The shaken polyethylene bag was placed in a household 350 L refrigerator and stored (seed cooling) at 10 ° C. (humidity 80%) for 30 days. During storage, the polyethylene bag was opened and then closed and shaken every 7 days. After storage, the mixture was taken out from the polyethylene bag and sieved with a sieve having a mesh size of 250 μm to separate the seeds.

  The separated seeds were coated by the same method as in Example 2 to produce coated seeds having an average particle size of 1.5 mmφ. The obtained coated seeds were dried with hot air at 30 ° C. for 20 hours. After sowing on a plug tray in a greenhouse (June), seedlings were grown for 40 days at 20-25 ° C. in the daytime and 14-16 ° C. at night.

  After the seedlings were planted in the field (August), they were cultivated for 70 days. A photograph of the cultivated strain is shown in FIG. As shown in FIG. 1, no rosetting was observed. The flowering rate was 98%.

Comparative Example 2
The seeds obtained after sieving and separation obtained in the same manner as in Example 3 were coated by the same method as in Example 2 without performing seed cooling to produce coated seeds having an average particle diameter of 1.5 mmφ. Thereafter, the cultivation of eustoma was carried out in the same manner as in Example 3, and rosette formation occurred. A photograph of the cultivated strain is shown in FIG. The flowering rate was 40%.

  From the results of Example 3 and Comparative Example 2, when water is added to seeds without using a carrier and seed cooling is performed, rosette formation is likely to occur and the flowering rate is low. However, it has been shown that when the method of the present invention is used to cool seeds by adding moisture to seeds using a carrier, rosette formation is prevented and a high flowering rate can be obtained.

Example 4
In a commercially available 100 ml polyethylene bag, 1 g of an Eustoma coat seed (average particle size: 1.5 mmφ) selected from commercially available products under the trade names shown below and 20 g of diatomaceous earth listed in Table 1 were added, and 0.1 After adding 6 g of an aqueous solution (moisture) containing mass% benrate T (bactericidal agent) (the addition amount of water to the carrier was 30 mass%), the polyethylene bag was closed and vigorously shaken for 1 minute.

[Eustoma coat seeds]
・ Product name: Wintage Marine (Miyoshi Corporation)
・ Product name: Moret Marine (Miyoshi Corporation)
・ Product name: Purple Flamingo (Miyoshi Corporation product)
・ Product name: Nightingale (Miyoshi Corporation)
・ Product name: Andura Vendor (Murakami Seed Co., Ltd.)
・ Product name: Ceremony White (Murakami Seed Co., Ltd.)
・ Product name: Fancy Lavender ELT (Murakami Seed)

  The shaken polyethylene bag was placed in a household 350 L refrigerator and stored (seed cooling) at 10 ° C. (humidity 80%) for 30 days. During storage, the polyethylene bag was opened and then closed and shaken every 7 days. After storage, the mixture was taken out from the polyethylene bag and sieved with a sieve having a mesh of 1000 μm to separate the seeds.

  For winter marine, mollet marine, purple flamingo and nightingale, after separating the seeds, 200 coat seeds were sown on a plug tray in a greenhouse (June) and then 20-25 ° C. in the daytime. During the night, the seedlings were grown at 14-16 ° C. for 40 days, the germination state was confirmed, and the germination rate (the ratio of germination in the sown seeds) was examined. The results are shown in Table 4. Furthermore, after raising seedlings, 100 seedlings were arbitrarily selected and planted in 2.5-inch pots in a greenhouse (August), then cultivated for 60 days, and visually checked for rosetting rate. The results are shown in Table 5. The rosetting rate is (number of rosettes / number of plants planted) × 100 (%).

  As for undulavender, ceremony white, and fancy lavender ELT, the germination rate and rosetting rate were examined in the same manner as in the case of Wintage Marine etc. except that seedlings were grown at 20 to 25 ° C. for 40 days. The results of germination rate are shown in Table 4, and the results of rosetting rate are shown in Table 5. Moreover, the photograph of the strain | stump | stock cultivated about ceremony white is shown in FIG.

Comparative Example 3
For the coated seeds (average particle size of 1.5 mmφ) selected from the commercial products of the above trade names, seeding was carried out in the same manner as in Example 4 except that 200 coated seeds were used and no seed cooling was performed. , Raising seedlings and cultivating, and examined the rosetting rate visually. The results are shown in Table 5. Moreover, the photograph of the strain | stump | stock cultivated about ceremony white is shown in FIG.

  The results of Example 4 and Comparative Example 3 indicate that rosette formation is likely to occur when seed cooling is not performed, but rosetting can be suppressed if seed cooling is performed using a carrier.

Claims (7)

Eustoma seeds, a carrier comprising water particles having a particle size that can be separated from the seeds, and a mixing step of mixing water, and a temperature at which the mixture obtained in the mixing step has a rosette-preventing effect and A seed cooling process for storing for a period of time , wherein the seed obtained by the seed cooling process is seeded, raised, planted, cultivated and cultivated to flower.
  2. The method for producing an elastomer according to claim 1, wherein a temperature exhibiting the effect of preventing rosette is a temperature within a range of 1 to 15 ° C. 3.   3. The method according to claim 1, further comprising a step of separating the seed and the carrier after the seed cooling step and before sowing, and coating the separated seed with an inorganic substance to obtain a coated seed. The production method of the described eusumma.   3. The method for producing an yeast according to claim 1 or 2, wherein the seed of the yeast is a coated seed, and the seed and the carrier are separated after the seed cooling step and before sowing.   5. The method for producing an eastoma according to claim 1, wherein the carrier is made of particles having a particle size smaller than that of the seed of the eastoma.   6. The method for producing an elastomer according to any one of claims 1 to 5, wherein the carrier is diatomaceous earth or alumina.   The method for producing an elastomer according to any one of claims 1 to 6, wherein a fungicide is contained in the mixture.