JP6042290B2 - Animal body shape improvement medicine - Google Patents

Description

  The present invention relates to an animal body shape-improving agent, and enables the breeding of high-performance animals having high performance such as high milk production, high reproduction, and high meat production.

  In order to raise animals such as calves after birth to high-capacity cattle (high milk production, high breeding, high meat production, etc.), by properly using nutrients at the time of fast growth after birth, It is important to make a good (big body, well balanced) cow. For this reason, the present invention is the first successful development of a material that can improve the body shape by promoting the use of basic nutrients taken.

  In order to promote the growth of calves, it is important to enhance the skeleton and muscles.

  However, the purpose cannot be achieved by simply feeding a large amount of feed or nutrients. On the contrary, growing cows with over-nutrition due to excessive nutrition are unlikely to be high-performance cows. Therefore, a material that enables effective use of appropriate nutrients is desired. Looking at the growth of calves after birth, the growth of bones and other vital parts for supporting the body and internal organs necessary for life support is fast, and the development of tissues that are not particularly necessary for life activities such as fat is the last. Become. Therefore, we focused on the point that the most important issue for the growth of calves is whether or not an appropriate skeleton is quickly formed after birth.

Skeletal formation is performed by bone formation and skeletal muscle formation.
The requirement for calves and growing cattle is to create a large, solid skeleton and a strong internal organ that are necessary to support future high milk production and meat production.

  For calves, the effects of malnutrition appear most quickly in body weight and circumference, and the slowest in body height and chest width. In general, the length of the body is affected more quickly than the height (“Introduction to Calf” p.16 Daily Japan Special Issue February 5, 1989).

Therefore, in order to improve the body shape, it is difficult to achieve high nutrition or low nutrition, and it is important to be able to use appropriate nutrients effectively. Obese and skinny children should be avoided.
The body type is evaluated by measuring each item such as nutrition (volume: chest width x chest depth x body length), body height, chest circumference, chest depth, buttocks length, tolerance, etc. This can be done by calculating the ratio.

  In the current state of the art, the present invention sets the purpose of improving body shape, and solves this by an oral composition such as feed or oral administration, and by combining the lactic acid bacteria culture and the propionic acid bacteria culture This is the first successful solution to this problem.

  Examples of the former, lactic acid bacteria culture (fermented product) include lactic acid bacteria and Lactobacillus gasseri fermentation products. Although this fermented product is known to have an action to improve the intestinal flora of calves (see, for example, Patent Document 1), it is not known to have a body shape improving action.

  Examples of the latter propionic acid bacteria culture (fermented product) include propionic acid bacteria and Propionibacterium fredenreich fermented product. Although this fermented product is known to have an action of promoting the growth of bifidobacteria in the intestine (see, for example, Patent Document 2), it is not known to have a body shape improving action.

  In addition, it is suggested that the mixture of Lactobacillus gasseri fermentation by-product and Propionibacterium flodenreich fermented product has the effect of improving the intestinal flora of newborn calves, and may enhance the intestinal immunity function of newborn calves. (For example, refer nonpatent literature 1) However, the point which improves a body shape is not described.

  Thus, although some actions are known by the combined use of lactic acid bacteria cultures and propionic acid bacteria cultures, it has not been known at all that the body shape is improved, and the present invention is first. Therefore, the present invention using both of these has succeeded in developing a completely new use (medicine effect) (so-called second use (medicine effect)) completely different from the conventionally known use (medicine effect). It is a use invention.

JP 2004-305128 A JP 2006-254841 A Takanori Masuko et al., Abstracts of the 107th Annual Meeting of the Japanese Society of Animal Science 2007 145

  The present invention has been made for the purpose of breeding animals having high performance such as high milk production, high reproduction, and high meat production.

  The present invention has been made to achieve the above object, and as a result of examination from various directions, it is important to care for the time of pups in order to produce high-performance animals, and the pups are simply fattened. We paid attention to the fact that it was necessary to make the puppy not only to have a good shape but also to have a good body shape (only a large body and good balance).

  Therefore, as a result of examinations from various directions regarding measures to improve body shape (that is, to improve body shape), forms that can be administered orally such as feeds, foods and drinks, orally administered drugs are from the viewpoint of convenience and safety. It was only from the point of view that it was advantageous. And from the standpoint of safety, we decided not to ask for an active ingredient for a new substance but for a known substance.

  A number of known substances were screened but were not successful. Therefore, the idea was changed to focus on the combined use of known substances, and more difficult screening was performed.

  As a result, we obtained new knowledge that the combined use of lactic acid bacteria culture (fermented lactic acid bacteria) and propionic acid bacteria (fermented propionic acid bacteria) was effective. The inventors have found that Lactobacillus gasseri and Propionibacterium fredenreich are suitable as propionic acid bacteria, and have completed the present invention.

That is, the present invention is characterized in that (A) a lactic acid bacterium (Lactobacillus gasseri) fermented product and (B) a propionic acid bacterium (Propionibacterium freudenreich) fermented product are used in combination. The embodiment is as follows.
(1) Animal length ratio: (body length / body height) × 100, hip length / body height, characterized in that (A) lactic acid bacteria fermentation product and (B) propionic acid bacteria fermentation product are used as active ingredients Ratio, at least one agent for increasing body weight / height ratio.
(A) Fermented lactic acid bacteria:
A fermented product prepared by neutralizing culture of Lactobacillus gasseri in a whey protein derivative-containing medium, or a concentrate thereof, or a dried product thereof.
(B) Propionic acid fermented product:
A fermented product prepared by culturing Propionibacterium fredenreich in whey and / or its protease-treated product, or a concentrate thereof, or a dried product thereof.
(2) Lactobacillus gasseri is Lactobacillus gasseri OLL 2716 (FERM BP-6999) strain, Propionibacterium Freudrenreich is Propionibacterium fleudenreich -3 (FERM BP-8115) strain, the agent according to (1).
(3) The agent according to (1) or (2), wherein the animal is a pup.
(4) The agent according to (3), wherein the larva is a calf.
(5) Body length ratio of livestock characterized by (O) fermented lactic acid bacteria and (B) fermented propionate fermented to animals, as described below: (body length / body height) × 100, hip length / At least one method for increasing the body height ratio, body weight / body height ratio.
(A) Fermented lactic acid bacteria:
A fermented product prepared by neutralizing culture of Lactobacillus gasseri in a whey protein derivative-containing medium, or a concentrate thereof, or a dried product thereof.
(B) Propionic acid fermented product:
A fermented product prepared by culturing Propionibacterium fredenreich in whey and / or its protease-treated product, or a concentrate thereof, or a dried product thereof.
(6) Lactobacillus gasseri is Lactobacillus gasseri OLL 2716 (FERM BP-6999) strain, Propionibacterium Freudenreich is Propionibacterium fredenchterenium -3 (FERM BP-8115) strain, the method according to (5).
(7) The method according to (6) or (7), wherein the livestock is a puppy.
(8) The method according to (7), wherein the larva is a calf.

  According to the present invention, it is known that (A) fermented lactic acid bacteria and (B) fermented propionic acid bacteria are orally administered (although they have an action of improving the intestinal flora of calves. It is not known to produce puppies with a good body shape.) It is possible to produce puppies with a well-balanced body shape.

  The pups produced in this way can be bred into high-capacity animals having high capacities such as high milk production, high breeding and high meat production by rearing them in accordance with ordinary methods. The body shape improving agent, body shape improving feed or body shape improving feed additive according to the present invention can be widely applied to various animals, and according to the present invention, the body is large and has a well-balanced body shape. It can be raised to healthy and highly capable animals, and humans are no exception.

  Therefore, according to the present invention, for example, a body shape improving agent is provided, but in addition to a body shape improving agent as an animal medicine such as livestock, a body shape improving agent for humans is also provided.

The change of the body weight in a prebio support salary test (Example 1) is shown. Similarly, the transition of DG (day weight gain) is shown. Similarly, changes in body size are shown. In the figure, the number on the horizontal axis indicates the age of the week, and the number on the vertical axis indicates cm. Similarly, the continuation of body size transition is shown. In the figure, the numbers on the horizontal axis indicate the age of the week, the numbers on the vertical axis indicate% in the case of the weight / body height ratio, and the numbers in the other graphs indicate cm. 1 to 4 indicate that there is a significant difference in a significant difference test (t-test) (*: p <0.05, **: p <0.01).

  The present invention relates to an animal body shape improver, body shape improved feed, or body shape improved feed additive characterized by using (A) a fermented lactic acid bacterium and (B) a fermented propionic acid bacterium described below together. The embodiment is as follows, for example.

(A) Fermented lactic acid bacteria Refers to fermented lactic acid bacteria (Lactobacillus gasseri).
For example, in a medium containing a whey protein derivative (eg, whey protein concentrate (WPC), whey protein isolate (WPI), or at least one of these hydrolysates), Lactobacillus gasseri (eg, OLL 2716) Strain: FERM BP-6999), a fermented product prepared by neutralization culture, or a concentrate thereof, or a dried product thereof.

(B) Propionic acid bacterium fermented product This refers to a propionic acid bacterium fermented product.
For example, a whey fermentation product of Propionibacterium fredenreich (for example, ET-3 strain: FERM BP-8115) can be mentioned. More specifically, a culture obtained by culturing the ET-3 strain in a whey (whey and / or protease-treated product) -containing medium (for example, whey powder reducing solution), isolated bacteria themselves, culture A whey fermentation product selected from at least one of a liquid (culture supernatant) and a mixture thereof.

(1) A method for producing a puppy having a good body shape for raising a high-capacity livestock, characterized in that (A) a fermented lactic acid bacterium and (B) a fermented propionic acid bacterium are orally administered simultaneously or separately.
(2) “Good body shape” means body balance, physique, nutrition (growth), cruciform height / body height ratio, body weight, cruciform height, body length, chest depth, hip length, hip angle width, generosity, ischia The method according to (1) above, which is at least one improvement in width and chest circumference.
(3) Improvement in balance of body is at least one increase in body length ratio: (body length x body height) x 100, chest circumference: (chest circumference / body height) x 100; (2) characterized in that the improvement in nutrition (growth) is at least one increase in body weight / body height ratio, body length / body height ratio, and volume (chest depth × chest width × body length) the method of.

(4) After producing a puppy having a good body shape by any one of the above methods (1) to (3), and raising it in accordance with a conventional method, a highly lactating livestock, a high breeding livestock, a high meat producing livestock (for example, , Cattle) at least one method of raising livestock.

(5) Cross section height / body height ratio, body length ratio, chest circumference ratio, buttocks length / body height ratio characterized by orally administering (A) lactic acid bacteria fermented product and (B) propionic acid bacteria fermented product to animals A method of increasing at least one of body weight / body height ratio, body length / body height ratio, and volume (chest depth × chest width × body length).
(6) (A) Fermented lactic acid bacteria and (B) Propionic acid fermented products are orally fed to calves for 25 days from birth, compared to control calves that do not orally feed the mixture. Increase at least one of body weight, cross height, body length, chest depth, hip length, hip width, tolerance, sitting height, chest circumference, and at the same time, cross height / body height ratio, body length ratio, chest circumference ratio, buttocks length A method of increasing at least one of: / body height ratio, body weight / body height ratio, body length / body height ratio, and volume (chest depth × chest width × body length).

(7) Lactobacillus gasseri (Lactobacillus gasseri) fermentation product and Propionibacterium fredenreich fermentation product as active ingredients, cruciform height / body height ratio, length ratio, chest ratio, hip length An animal body shape improver characterized by increasing at least one of: / body height ratio, body weight / body height ratio, body length / body height ratio, and volume (chest depth × chest width × body length).
The present invention includes at least one of a body shape improver as a medicine, a body shape improver as an animal medicine excluding humans, a feed having a body shape improving action, and the same feed additive.

  In order to raise a puppy to a high-capacity livestock (at least one of high milk production, high breeding, high meat production, etc.), a puppy having a good body shape (large body and good balance) is required. This point is also pointed out in the following references.

  In fact, in the case of cattle, for example, the 2004 Beef Cattle Long-Term Central Workshop Text, “Issues for Wagyu Beef Improvement and Wagyu Beef Review”, July 26-28, 2004, Japan Beef Cattle Promotion Foundation, 1 to 18 (Document 1), 2004 Beef Cattle Long-Term Central Workshop, “Review of Wagyu Beef”, Hokkaido Wagyu Register Association Hokkaido Branch, pages 1 and 4 (Document 2) Review is being conducted.

  According to it, physique screening is conducted based on the screening standards, and for calves, avoiding obese and skinny children and selecting cattle that will be easier to keep in the future (specifically, as “how to view and choose calves”) Include a large skeleton, a high cruciform height compared to the body height, a wide calf-like body width and body depth), and the choice of a cow that does not lose its body shape. In addition, it is also mentioned that the balance (breadth x body depth x body length) and the common name are preferable according to the age of the moon.

  Thus, as a calf to be a high-capacity cow, not only is each part large and the body is large, but also a balance and a good body shape are desired.

  The present invention sets the technical problem of “producing” artificially, in other words, actively producing, rather than relying on natural things such as mating and screening for calves with good body shapes. In terms of safety and convenience, we decided to develop a new oral administration.

  And, as a result of examination from various directions, the present inventors have used a fermented product of Lactobacillus gasseri, which is a lactic acid bacterium, and a fermented product of Propionibacterium freudenreich, which is a propionic acid bacterium, so that the calf body shape Was found for the first time and the present invention was completed.

  That is, the present invention is characterized in that both of these fermented products are used in combination, and the present invention is a method for producing puppies with good body shape, a method for breeding high-performance livestock, a method for improving body shape, and a body shape improvement. The present invention provides an agent, a body shape improving medicine, a body shape improving animal medicine, a body shape improving feed, and a body shape improving feed additive.

  As a Lactobacillus gasseri fermentation product, a fermented product prepared by neutralizing Lactobacillus gasseri (Lactobacillus gasseri) in a whey protein derivative-containing medium, a concentrate thereof, or a dried product thereof can be used. .

  More specifically, the fermented product includes, for example, a fermented product obtained by culturing Lactobacillus gasseri, for example, OLL 2716 strain (FERM BP-6999) (a whole culture containing a cell, a medium component, a metabolic secretion, etc.). ), Bacterial cells separated from them, fermented product residue obtained by removing solids containing bacterial cells from the culture (culture liquid, culture supernatant that is not a turbid part of the culture liquid but a clear part Processed product (concentrate, pasted product, dried product (at least one of spray dried product, freeze dried product, vacuum dried product, drum dried product), liquid product, diluted product, sterilized product) At least one selected from.

As the medium, synthetic medium, semi-synthetic medium and other commonly used medium can be used as appropriate. The gasseri high-concentration culture solution uses a medium containing whey protein derivatives and / or lactose, particularly a medium mainly composed of whey protein derivatives and / or lactose, and the pH is in the range of 4.5 to 7.0. It is obtained by carrying out neutralization culture until the number of bacteria becomes 10 ⁸ to 10 ¹⁰ , preferably 10 ⁹ to 10 ¹¹ . In the case of a normal culture method, the number of bacteria in the culture is only 10 ⁹ or less.

  As the whey protein derivative, at least one selected from whey protein concentrate (Whey Protein Concentrate, WPC), whey protein isolate (Whey Protein Isolate, WPI), and a hydrolyzate thereof can be used, and commercially available products can also be used as appropriate. It is.

  WPC is obtained by treating whey produced in the production of cheese and casein by methods such as ultrafiltration, gel filtration, and lactose crystal separation, and increasing the protein content to usually 35 to 85% (in terms of solid content). . WPI is distinguished from WPC and has a protein content increased to about 95% (in terms of solid content) by a method such as an ion exchange method.

  As the hydrolyzate, an enzyme-treated product obtained by treating at least one selected from WPC, WPI, whey, and whey protein with an enzyme such as a proteolytic enzyme such as pepsin, trypsin, or papain is used.

  Examples of the fermented product include a whey protein-containing medium which is a fermented product described in JP-A-2004-305128 (Patent Document 1). A fermented product (solid content 10.8%) prepared by neutralizing culture of gasseri OLL 2716 (FERM BP-6999) can be used.

  The dose of fermented Lactobacillus gasseri bacteria per day per calf is 0.93 to 92.59 g (0.1 to 10 g in terms of solid content), preferably 4.65 to 46.3 g (solid In terms of minutes, it is 0.5 to 5 g). In the case of other animals, the dose may be increased or decreased in proportion to the body weight.

  By adding excipients (starch, dextrin, milk component, silicic acid, etc.) to Lactobacillus gasseri fermented product and spray-drying, it is possible to make a composition containing fermented Lactobacillus gasseri fungus that is easy to use. This can also be used as the main fermented product.

  Although it is known that the fermented Lactobacillus gasseri fungus has an action to improve the intestinal flora of calves (Patent Document 1), it is not known at all to have an action to improve body shape.

  As a propionic acid bacteria fermented product, a fermented product prepared by culturing Propionibacterium fredenreich in a medium containing whey and / or its protease-treated product, or a concentrate thereof, or a dried product thereof is used. can do.

  In more detail, the fermented product is a culture obtained by culturing Propionibacterium floudenreich, for example, ET-3 strain (FERM BP-8115). The same fermented Bacillus gasseri is used.

  As this fermented product, a whey-containing medium (for example, a 10% whey powder reducing solution) that is a fermented product described in JP-A-2006-254841 (Patent Document 2) is used as a Propionibacterium fredenreich ET-3 strain (FERM BP-8115). ) And fermented and prepared. This contains 900 μg of DHNA (1,4-dihydroxy-2-naphthoic acid: 1,4-dihydroxy-2-naphthoic acid) per 100 g.

  An easy-to-use propionic acid bacterium fermented product-containing composition can be made by adding an excipient (fat dry milk, starch, flour, sugar, etc.) to this propionic acid bacterium fermented product and freeze-drying. One gram of this product contains 60 μg of DHNA.

  The dose of fermented propionic acid bacteria per calf per day is 0.27 to 67.5 g, preferably 0.675 to 27 g. In the case of other animals, the dose may be increased or decreased in proportion to the body weight.

  When using a propionic acid bacteria fermented product-containing composition, the dose is 0.04 to 10 g, preferably 0.1 to 4 g. When these doses are converted into DHNA amounts, they are 2.43 to 607.5 μg, preferably 6.07 to 243 μg.

  Although the propionic acid bacteria fermented product contains a bifidobacteria growth promoting component and is known to have an action to improve the intestinal flora of calves (Patent Document 2), Not known at all.

  In the present invention, the propionic acid bacteria fermentation product and the lactic acid bacteria fermentation product are used in combination. These fermented products may be used separately or may be used simultaneously as a mixture. The mixing ratio of the fermentation product of propionic acid bacteria and the fermentation product of Lactobacillus gasseri is 10: 1 to 1: 100, preferably 5: 1 to 1:50.

  A commercial product can also be used as a mixture of these two fermented products. As a commercial product, for example, a mixture of Lactobacillus gasseri OLL 2716 fermentation by-product concentrate (LG) and a fermented product (propionic acid bacteria whey fermented product) by Propionibacterium Freudenreich ET-3 is dextrin, lactose Prebio Support (PS: trade name of Meiji Dairy Co., Ltd.), which is a powder product obtained by formulating with silicon dioxide, can be used.

  PS (trade name) has been suggested to enhance the intestinal immunity of Japanese black calves (“Lactic acid bacteria fermentation by-product / propionic acid fermented fertilizer mixture contributes to intestinal immunity of newborn calves” “Influence” Takanori Masuko et al., Abstracts of the 107th Annual Meeting of the Japanese Society of Animal Science 2007 (p.145). However, it is not known at all that PS has the effect of improving calf body shape.

  As described above, the present invention has newly found a new use or medicinal effect (second use or medicinal effect) that is completely different from the conventional fermented product of lactic acid bacteria and propionic acid bacteria. Invents the invention.

  Therefore, the present invention comprises both of these fermented products as active ingredients and contains them as they are, or is further used as an orally used pharmaceutical excipient (adjuvant), as well as an animal pharmaceutical excipient (adjuvant). ) Is used to provide a body shape improving agent (body shape improving medicine, body shape improving animal medicine).

  In addition, the present invention uses both of these fermented products as they are as feeds or feed additives, and also adds an excipient (fat dry milk, lactose, starch, flour, saccharides, silicon dioxide, etc.) to these both fermented products. ) And feed ingredients to obtain feed, which may be orally administered to pups. It is also possible to mix with feed water or milk substitute. Therefore, this invention also provides the feed or feed additive containing both these fermented products.

  The present invention also relates to a method of orally administering these fermented products to improve the body shape of animals, particularly puppies, and to produce puppies with good body shapes (large body, good balance and balance). It is.

  In the present invention, “good body shape” means balance of body, physique, nutrition (growth), cross height / height ratio, weight, cross height, body length, chest depth, hip length, hip angle width, generosity It means improvement of at least one of sciatic width and chest circumference.

  In the present invention, the improvement of the balance of the body is at least one increase of the body length ratio: (body length / body height) × 100 and the chest circumference: (chest circumference / body height) × 100. An increase in the length / body height ratio, and improvement in nutrition (growth) refers to an increase in at least one of body weight / body height ratio, body length / body height ratio, and volume (chest depth × chest width × body length).

  Therefore, the present invention provides a method for improving a body type, a method for increasing each item (each site) and / or its ratio relating to the body type, and a puppy thus improved for the body type according to a conventional method. This also provides a method for raising high-capacity livestock.

  In the case of calves, the composition containing the fermented propionic acid bacteria and the fermented Lactobacillus gasseri to the larvae will be weaned until weaning at about 8 weeks of age from the time when the skeleton is rapidly formed. This period is appropriate. In the case of other larvae, it may be appropriately defined with reference to the calf period.

  Examples of the present invention will be described in detail below, but the present invention is not limited to these examples.

  In this example, a commercially available product (Prebio Support: PS) was used as a mixture of a lactic acid bacterium fermentation product and a propionic acid bacterium fermentation product. Prebio Support (PS) is a trade name of Meiji Dairies Co., Ltd. As described above, a mixture of Lactobacillus gasseri OLL 2716 fermentation by-product and Propionibacterium Freudenreich ET-3 fermentation product It is a powder product.

  Prebiosupport (PS) is known for its effects on improving intestinal flora and enhancing intestinal immunity, but it has the effect of improving the balance and balance of the body and producing well-formed pups. Is not known or suggested.

In this example, the effect of improving body shape due to PS was confirmed not by an in vitro test but by an in vivo test using an actual calf as a livestock.

1. The active ingredients contained in the daily dose of PrebioSupport (PS) are as follows.
Lactobacillus gasseri fermented product (converted to solid matter): 1.25 g × 2 times = 2.5 g (23.15 g as Lactobacillus gasseri fermented product)
Propionibacterium fredenreichii fermented product-containing composition: 0.2 g (containing 12 μg of DHNA) × 2 times = 0.4 g (containing 24 mg of DHNA) (2.7 g for Propionibacterium fredenreichii fermented product)

2. PS salary test: Test conditions-Test cattle: 20 Wagyu beef (10 PS added, 10 not added)
・ PS feeding from the first day of delivery in the addition zone ・ Switch to feeding with automatic feeding machine from the 5th day ・ PS addition period is 25 days

  3. In addition to changes in body weight, select items (body height, cross height, body length, chest depth, chest width, hip width, hip width, hip width, sciatic width, chest circumference, abdominal circumference) that can be evaluated. , Conducted according to conventional methods.

  4). Test results: as follows.

  -At 8 weeks of age, PS group> control group were weight, cross height, body length, chest depth, hip length, hip width, hip width, sciatic width, chest circumference, weight / body height ratio.

  ・ At 8 weeks of age, there were no significant differences in body height, chest width, waist circumference, and waist / chest circumference ratio.

In the administration of PS, the skeleton becomes larger and the cross height is higher than the body height. In addition, since the chest depth and chest width have increased, it is sufficiently predicted that the cow will be easy to keep and have a high utilization rate of feed.
These things lead to the breeding of high lactating cows and high beef producing cows.

  Changes in blood components also appeared as follows, and nutrient metabolism was promoted.

・ Data with significant difference at 8 weeks of age:
ALP is PS group> control group: Ca metabolism promotion T-Chol is PS group> control group: lipid metabolism promotion Phospholipid is PS group> control group: lipid metabolism promotion BUN is PS group <control group: protein metabolism Promotion

  In the table, ALP indicates alkaline phosphatase, T-Chol indicates total cholesterol, and BUN indicates urea nitrogen. From the above results, PS was considered to be involved in metabolic systems such as lipids and proteins in addition to Ca metabolism.

<Body shape improvement effect>
Body type (balance of body) was evaluated by body length rate and chest circumference.

Reference 1: “Production Veterinary Medical System Dairy Cattle 1” p. 27 Issued January 25, 1999 by the Agricultural and Fishing Village Cultural Association.
Changes in body balance are indicated by body length ratio: (body length / body height) × 100 and chest circumference: (chest circumference / body height) × 100. The balance of the body at the age of 2 months is the basis for the balance of the body after that. Therefore, it is important to create an ideal balance (form) immediately after birth.

-Evaluation of PS administration according to the above results:
Comparison of body length ratio (8 weeks old)
Control group: body length ratio = (body length / body height) × 100 = (69/79) × 100 = 87.3%
PS ward: Body length ratio = (body length / body height) x 100 = (86/83) x 100 = 103.6%
Comparison of chest circumference (8 weeks old)
Control group: Chest circumference ratio = (chest circumference / body height) × 100 = (86/70) × 100 = 108.9%
PS Ward: Chest circumference = (chest circumference / body height) x 100 = (92/83) x 100 = 110.8%

  As shown above, the body shape (balance of the body) is clearly improved by the administration of PS.

<Body improvement effect>
Assessed by the ratio of hip length / body height.

・ Reference: “Examination of causes of stillbirth in dairy cattle: Approach from physique measurement results” Kazuhiro Yamakawa et al. 7
In dairy cows, the stillbirth rate was higher in the group with a lower buttocks / height ratio. Therefore, there is a need to improve the body shape so as not to lower the butt length / body height ratio.

  -Evaluation of PS administration based on the above results: Comparison at 8 weeks of age

  As is clear from the above results, with the administration of PS, the hip length / height ratio progressed in the direction of improvement.

<Nutrition improvement (growth improvement) effect>
(1) The weight / body height ratio was evaluated.

・ Reference: “Study on poor growth in Japanese black cattle” Takasu, Masa et al., 143rd Annual Meeting of the Japanese Veterinary Medical Society, April 3, 2007, Abstracts of Lectures p. 63
Japanese black cattle often develop poorly. In the case of poor growth due to a non-endocrine disease, the body weight is light with respect to the body height, indicating a truncated shape. Underdeveloped cattle do not have debilitating diseases such as diarrhea and pneumonia, and are in an energy-deficient state despite taking enough feed for normal growth.

  -Evaluation of PS administration according to the above results:

  As is clear from the above results, the body weight / body height ratio of calves was clearly increased by the administration of PS, and the effect of nutrition improvement was observed.

(2) The body length / body height ratio was evaluated.
It is known that the length / height ratio of healthy calves is greater than that of unhealthy calves (bovines with persistently infected bovine viral diarrhea virus).
Masaaki Koiwa, Clinical Veterinarian 24 (4) 42-47, 2006

  -Evaluation of PS administration according to the above results:

  As is clear from the above results, the effect of improving nutrition (growth improvement) was clearly seen with the administration of PS.

(3) Nutritional status was evaluated by volume (chest depth x chest width x body length).
The volume is usually an item to be measured when evaluating the nutritional level.

  Evaluation of PS administration according to the above results:

  As is clear from the above results, the nutritional level is improved by the administration of PS.

  Therefore, it is considered that the effects of administration of PS to calves were related to the action of various nutrients in addition to the action of improving intestinal flora and immunostimulatory action, and the action of enhancing the metabolic activity of various nutrients. In particular, in addition to Ca metabolism, an effect on the metabolic system of nutrients such as fat and protein is expected. Although the calf has been described above as a representative example, the present invention is similarly applied to pups such as horses, sheep, goats, camels, and buffalos other than calves.

Claims (4)

(A) Fermented product of lactic acid bacteria and (B) Propionic acid fermented product as an active ingredient to be described below: Body length ratio of animal: (body length / body height) × 100, hip length / body height ratio, body weight / An orally administered pharmaceutical agent for increasing at least one body height ratio.
(A) Fermented lactic acid bacteria:
A fermented product prepared by neutralizing culture of Lactobacillus gasseri in a whey protein derivative-containing medium, or a concentrate thereof, or a dried product thereof.
(B) Propionic acid fermented product:
A fermented product prepared by culturing Propionibacterium fredenreich in whey and / or its protease-treated product, or a concentrate thereof, or a dried product thereof.   Lactobacillus gasseri is Lactobacillus gasseri OLL 2716 (FERM BP-6999) strain, Propionibacterium Freudenreich is Propionibacterium freden-3 It is a FERM BP-8115) strain | stump | stock, The agent of Claim 1 characterized by the above-mentioned.   The agent according to claim 1 or 2, wherein the animal is a puppy.   4. The agent according to claim 3, wherein the larva is a calf.

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