JP6021813B2 - 多重増幅および検出 - Google Patents

多重増幅および検出 Download PDF

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JP6021813B2
JP6021813B2 JP2013535508A JP2013535508A JP6021813B2 JP 6021813 B2 JP6021813 B2 JP 6021813B2 JP 2013535508 A JP2013535508 A JP 2013535508A JP 2013535508 A JP2013535508 A JP 2013535508A JP 6021813 B2 JP6021813 B2 JP 6021813B2
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probe
oligonucleotide
amplification
probes
melting
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JP2013540449A (ja
JP2013540449A5 (OSRAM
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グオリアン フー
グオリアン フー
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オキシテック リミテッド
オキシテック リミテッド
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6858Allele-specific amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00Libraries per se, e.g. arrays, mixtures
    • C40B40/04Libraries containing only organic compounds
    • C40B40/06Libraries containing nucleotides or polynucleotides, or derivatives thereof

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
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  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2013535508A 2010-10-25 2011-10-25 多重増幅および検出 Active JP6021813B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1017978.6A GB201017978D0 (en) 2010-10-25 2010-10-25 Multiplex amplification and detection
GB1017978.6 2010-10-25
PCT/GB2011/052068 WO2012056227A2 (en) 2010-10-25 2011-10-25 Multiplex amplification and detection

Publications (3)

Publication Number Publication Date
JP2013540449A JP2013540449A (ja) 2013-11-07
JP2013540449A5 JP2013540449A5 (OSRAM) 2014-12-11
JP6021813B2 true JP6021813B2 (ja) 2016-11-09

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Family Applications (1)

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JP2013535508A Active JP6021813B2 (ja) 2010-10-25 2011-10-25 多重増幅および検出

Country Status (5)

Country Link
US (2) US9284607B2 (OSRAM)
EP (2) EP2633072A2 (OSRAM)
JP (1) JP6021813B2 (OSRAM)
GB (1) GB201017978D0 (OSRAM)
WO (1) WO2012056227A2 (OSRAM)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201017978D0 (en) 2010-10-25 2010-12-08 Oxitec Ltd Multiplex amplification and detection
EP2880184B1 (en) 2012-08-03 2021-03-31 California Institute of Technology Multiplexing and quantification in pcr with reduced hardware and requirements
EP2889381A1 (en) * 2013-12-27 2015-07-01 Universite De Liege Detection method for species specific identification of nucleic acid of interest
WO2015147370A1 (en) * 2014-03-28 2015-10-01 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures
WO2016093620A1 (en) 2014-12-09 2016-06-16 Seegene, Inc. Differentiation of signals for target nucleic acid sequences
KR102110985B1 (ko) * 2015-12-15 2020-05-14 주식회사 씨젠 타겟 핵산 서열에 대한 시그널 추출
KR102110999B1 (ko) * 2016-01-26 2020-05-14 주식회사 씨젠 타겟 핵산 서열에 대한 시그널을 제공하는 방법
US20200157601A1 (en) * 2016-12-02 2020-05-21 Congen Biotechnologie Gmbh Probe for detection of snps
WO2019144107A1 (en) * 2018-01-22 2019-07-25 Luminex Corporation Methods and compositions for discrete melt analysis
US12454720B2 (en) 2018-04-17 2025-10-28 ChromaCode, Inc. Methods and systems for multiplex analysis
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
MX2021001385A (es) 2018-08-09 2021-06-23 Speedx Pty Ltd Detección múltiplex de ácidos nucleicos.
WO2020206509A1 (en) * 2020-06-30 2020-10-15 Speedx Pty Ltd Multiplex detection of nucleic acids using mixed reporters

Family Cites Families (25)

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EP0232967B1 (en) 1986-01-10 1993-04-28 Amoco Corporation Competitive homogeneous assay
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
DK1179600T3 (da) 1996-06-04 2005-09-05 Univ Utah Res Found Overvågning af hybridisering under PCR
US6117635A (en) 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
US6140054A (en) 1998-09-30 2000-10-31 University Of Utah Research Foundation Multiplex genotyping using fluorescent hybridization probes
US6472156B1 (en) 1999-08-30 2002-10-29 The University Of Utah Homogeneous multiplex hybridization analysis by color and Tm
CA2417986C (en) 2000-08-11 2013-11-26 University Of Utah Research Foundation Single-labeled oligonucleotide probes
US7785776B2 (en) 2002-05-13 2010-08-31 Idaho Technology, Inc. Genotyping by amplicon melting curve analysis
US20040022764A1 (en) 2002-07-31 2004-02-05 Hanan Polansky Inhibition of microcompetition with a foreign polynucleotide as treatment of chronic disease
WO2004074447A2 (en) 2003-02-18 2004-09-02 Applera Corporation Compositions and methods for multiplex analysis of polynucleotides
US20050053950A1 (en) 2003-09-08 2005-03-10 Enrique Zudaire Ubani Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons
JP5162133B2 (ja) * 2003-12-03 2013-03-13 アボット・ラボラトリーズ 二本鎖直鎖核酸プローブ及びその使用
CA2580212C (en) 2004-09-24 2013-11-12 Universitaet Bern Molecular beacons
EP2927238B1 (en) * 2004-10-18 2018-01-17 Brandeis University Methods for LATE amplification and sequencing of a nucleic acid
CA2594223C (en) 2005-01-03 2016-03-15 The Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention Primer for nucleic acid detection
WO2007045890A1 (en) 2005-10-21 2007-04-26 Primerdesign Ltd Probe
JP2010505438A (ja) 2006-10-09 2010-02-25 オキシテック・リミテッド 核酸配列を増幅および検出する方法
PT1911852E (pt) 2006-10-12 2009-10-09 Bio Rad Pasteur Sondas de cadeia dupla para a detecção fluorescente de ácidos nucleicos
GB0703997D0 (en) 2007-03-01 2007-04-11 Oxitec Ltd Methods for detecting nucleic sequences
CA2680121C (en) * 2007-03-08 2016-05-10 Idaho Technology Primers for melting curve analysis in methods of nucleic acid analysis
GB0909333D0 (en) 2009-06-01 2009-07-15 Fu Guoliang Multiplex amplification and detection
US9234232B2 (en) 2008-07-31 2016-01-12 Oxitec Limited Multiplex amplification and detection
GB201017978D0 (en) 2010-10-25 2010-12-08 Oxitec Ltd Multiplex amplification and detection

Also Published As

Publication number Publication date
US10400276B2 (en) 2019-09-03
US9284607B2 (en) 2016-03-15
WO2012056227A3 (en) 2012-11-22
WO2012056227A2 (en) 2012-05-03
EP3150726B1 (en) 2019-08-28
GB201017978D0 (en) 2010-12-08
JP2013540449A (ja) 2013-11-07
EP2633072A2 (en) 2013-09-04
US20130267434A1 (en) 2013-10-10
US20160289751A1 (en) 2016-10-06
EP3150726A1 (en) 2017-04-05

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