JP6021813B2 - 多重増幅および検出 - Google Patents

多重増幅および検出 Download PDF

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JP6021813B2
JP6021813B2 JP2013535508A JP2013535508A JP6021813B2 JP 6021813 B2 JP6021813 B2 JP 6021813B2 JP 2013535508 A JP2013535508 A JP 2013535508A JP 2013535508 A JP2013535508 A JP 2013535508A JP 6021813 B2 JP6021813 B2 JP 6021813B2
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probe
oligonucleotide
amplification
probes
melting
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JP2013540449A5 (OSRAM
JP2013540449A (ja
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グオリアン フー
グオリアン フー
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オキシテック リミテッド
オキシテック リミテッド
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6858Allele-specific amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00Libraries per se, e.g. arrays, mixtures
    • C40B40/04Libraries containing only organic compounds
    • C40B40/06Libraries containing nucleotides or polynucleotides, or derivatives thereof

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2013535508A 2010-10-25 2011-10-25 多重増幅および検出 Active JP6021813B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1017978.6 2010-10-25
GBGB1017978.6A GB201017978D0 (en) 2010-10-25 2010-10-25 Multiplex amplification and detection
PCT/GB2011/052068 WO2012056227A2 (en) 2010-10-25 2011-10-25 Multiplex amplification and detection

Publications (3)

Publication Number Publication Date
JP2013540449A JP2013540449A (ja) 2013-11-07
JP2013540449A5 JP2013540449A5 (OSRAM) 2014-12-11
JP6021813B2 true JP6021813B2 (ja) 2016-11-09

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JP2013535508A Active JP6021813B2 (ja) 2010-10-25 2011-10-25 多重増幅および検出

Country Status (5)

Country Link
US (2) US9284607B2 (OSRAM)
EP (2) EP3150726B1 (OSRAM)
JP (1) JP6021813B2 (OSRAM)
GB (1) GB201017978D0 (OSRAM)
WO (1) WO2012056227A2 (OSRAM)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201017978D0 (en) 2010-10-25 2010-12-08 Oxitec Ltd Multiplex amplification and detection
EP3901243A1 (en) 2012-08-03 2021-10-27 California Institute of Technology Multiplexing and quantification in pcr with reduced hardware and requirements
EP2889381A1 (en) * 2013-12-27 2015-07-01 Universite De Liege Detection method for species specific identification of nucleic acid of interest
WO2015147370A1 (en) 2014-03-28 2015-10-01 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures
CN107208139B (zh) 2014-12-09 2020-11-10 Seegene株式会社 与靶核酸序列有关的信号的分辨
KR102110985B1 (ko) * 2015-12-15 2020-05-14 주식회사 씨젠 타겟 핵산 서열에 대한 시그널 추출
KR102110999B1 (ko) * 2016-01-26 2020-05-14 주식회사 씨젠 타겟 핵산 서열에 대한 시그널을 제공하는 방법
US20200157601A1 (en) * 2016-12-02 2020-05-21 Congen Biotechnologie Gmbh Probe for detection of snps
CA3088250A1 (en) * 2018-01-22 2019-07-25 Luminex Corporation Methods and compositions for discrete melt analysis
US12454720B2 (en) 2018-04-17 2025-10-28 ChromaCode, Inc. Methods and systems for multiplex analysis
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
WO2020031156A1 (en) 2018-08-09 2020-02-13 Speedx Pty Ltd Multiplex detection of nucleic acids
AU2020256848A1 (en) * 2020-06-30 2022-10-27 Speedx Pty Ltd Multiplex detection of nucleic acids using mixed reporters

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ATE88761T1 (de) 1986-01-10 1993-05-15 Amoco Corp Kompetitiver homogener test.
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
DE69739357D1 (de) 1996-06-04 2009-05-28 Univ Utah Res Found Überwachung der hybridisierung während pcr
US6117635A (en) 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
US6140054A (en) 1998-09-30 2000-10-31 University Of Utah Research Foundation Multiplex genotyping using fluorescent hybridization probes
US6472156B1 (en) 1999-08-30 2002-10-29 The University Of Utah Homogeneous multiplex hybridization analysis by color and Tm
CA2417986C (en) 2000-08-11 2013-11-26 University Of Utah Research Foundation Single-labeled oligonucleotide probes
US7785776B2 (en) 2002-05-13 2010-08-31 Idaho Technology, Inc. Genotyping by amplicon melting curve analysis
US20040022764A1 (en) 2002-07-31 2004-02-05 Hanan Polansky Inhibition of microcompetition with a foreign polynucleotide as treatment of chronic disease
US20040229253A1 (en) 2003-02-18 2004-11-18 Hyldig-Nielsen Jens J. Compositions and methods for multiplex analysis of polynucleotides
US20050053950A1 (en) 2003-09-08 2005-03-10 Enrique Zudaire Ubani Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons
CA2548205C (en) * 2003-12-03 2017-06-27 Abbott Laboratories Double stranded linear nucleic acid probe and uses thereof
EP1799853A2 (en) 2004-09-24 2007-06-27 Universität Bern Molecular beacons
RU2460804C2 (ru) * 2004-10-18 2012-09-10 Брандейс Юнивесити Способ гомогенной детекции по меньшей мере одного продукта одноцепочечной амплификации
US20060188902A1 (en) 2005-01-03 2006-08-24 The Gov. of the USA as represented by the Secretary of the Dept. of Health and Human Primer for nucleic acid detection
WO2007045890A1 (en) 2005-10-21 2007-04-26 Primerdesign Ltd Probe
EP2069540B1 (en) 2006-10-09 2014-03-19 Oxitec Limited Methods for amplifying and detecting nucleic acid sequences
EP1911852B1 (en) 2006-10-12 2009-07-29 Bio-Rad Pasteur Double-stranded probes for the fluorescent detection of nucleic acids
GB0703997D0 (en) 2007-03-01 2007-04-11 Oxitec Ltd Methods for detecting nucleic sequences
CN101918587B (zh) * 2007-03-08 2013-03-27 拜欧法诊断有限公司 用于熔解分析的引物
US9234232B2 (en) 2008-07-31 2016-01-12 Oxitec Limited Multiplex amplification and detection
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GB201017978D0 (en) 2010-10-25 2010-12-08 Oxitec Ltd Multiplex amplification and detection

Also Published As

Publication number Publication date
US20160289751A1 (en) 2016-10-06
WO2012056227A3 (en) 2012-11-22
EP3150726A1 (en) 2017-04-05
US20130267434A1 (en) 2013-10-10
EP3150726B1 (en) 2019-08-28
US9284607B2 (en) 2016-03-15
JP2013540449A (ja) 2013-11-07
US10400276B2 (en) 2019-09-03
EP2633072A2 (en) 2013-09-04
WO2012056227A2 (en) 2012-05-03
GB201017978D0 (en) 2010-12-08

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