JP5969921B2 - 抵抗性昆虫の対応のためのＣｒｙ１Ｂｅと組み合わせたＣｒｙ１Ｄａの使用 - Google Patents
抵抗性昆虫の対応のためのＣｒｙ１Ｂｅと組み合わせたＣｒｙ１Ｄａの使用 Download PDFInfo
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
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- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/50—Isolated enzymes; Isolated proteins
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
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- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
- C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
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| MX2014001456A (es) * | 2011-08-05 | 2014-02-27 | Dow Agrosciences Llc | Uso de proteina cristalina insecticida dig3 en combinacion con cry1ab. |
| IN2015DN03218A (de) * | 2012-10-05 | 2015-10-02 | Dow Agrosciences Llc | |
| US10968446B2 (en) | 2012-11-01 | 2021-04-06 | Massachusetts Institute Of Technology | Directed evolution of synthetic gene cluster |
| EP2971000A4 (de) | 2013-03-15 | 2016-11-23 | Pioneer Hi Bred Int | Phi-4-polypeptide und verfahren zu deren verwendung |
| EA030896B1 (ru) | 2013-08-16 | 2018-10-31 | Пайонир Хай-Бред Интернэшнл, Инк. | Инсектицидные белки и способы их применения |
| EP4159028A1 (de) | 2013-09-13 | 2023-04-05 | Pioneer Hi-Bred International, Inc. | Insektizidproteine und verfahren zu deren verwendung |
| BR112016018103B1 (pt) | 2014-02-07 | 2024-01-16 | E.I. Du Pont De Nemours And Company | Polipeptídeo e seu uso, polinucleotídeo, composição, proteína de fusão, método para controlar uma população, método para inibir o crescimento, método para controlar a infestação, método para obtenção de uma planta ou célula vegetal, construto |
| EP3705489A1 (de) | 2014-02-07 | 2020-09-09 | Pioneer Hi-Bred International, Inc. | Insektizide proteine und verfahren zu deren verwendung |
| AR099800A1 (es) | 2014-03-21 | 2016-08-17 | Agrigenetics Inc | Cry1d para controlar el gusano de la mazorca del maíz |
| WO2016000237A1 (en) | 2014-07-03 | 2016-01-07 | Pioneer Overseas Corporation | Plants having enhanced tolerance to insect pests and related constructs and methods involving insect tolerance genes |
| RU2733898C2 (ru) | 2014-10-16 | 2020-10-09 | Пайонир Хай-Бред Интернэшнл, Инк. | Инсектицидные белки и способы их применения |
| EP3267796B1 (de) | 2015-03-11 | 2023-08-09 | Pioneer Hi-Bred International, Inc. | Insektizide kombinationen von pip-72 und verfahren zur verwendung |
| BR112017024948A2 (pt) | 2015-05-19 | 2018-07-31 | Pioneer Hi Bred Int | proteínas inseticidas e métodos para uso das mesmas |
| US10647995B2 (en) | 2015-06-16 | 2020-05-12 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
| EP3322679A4 (de) | 2015-07-13 | 2019-07-10 | Pivot Bio, Inc. | Verfahren und zusammensetzungen zur verbesserung von pflanzeneigenschaften |
| CA2994676A1 (en) | 2015-08-06 | 2017-02-09 | Pioneer Hi-Bred International, Inc. | Plant derived insecticidal proteins and methods for their use |
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| WO2017062412A1 (en) | 2015-10-05 | 2017-04-13 | Massachusetts Institute Of Technology | Nitrogen fixation using refactored nif clusters |
| CN108575091A (zh) | 2015-12-18 | 2018-09-25 | 先锋国际良种公司 | 杀昆虫蛋白及其使用方法 |
| MX2018012025A (es) | 2016-04-14 | 2019-02-07 | Pioneer Hi Bred Int | Polipeptidos insecticidas que tienen un espectro de actividad mejorado y sus usos. |
| CA3021391A1 (en) | 2016-04-19 | 2017-10-26 | Pioneer Hi-Bred International, Inc. | Insecticidal combinations of polypeptides having improved activity spectrum and uses thereof |
| EP3451837B1 (de) | 2016-05-04 | 2021-08-25 | Pioneer Hi-Bred International, Inc. | Insektizide proteine und verfahren zu deren verwendung |
| CA3022858A1 (en) | 2016-06-16 | 2017-12-21 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
| RU2019101793A (ru) | 2016-06-24 | 2020-07-24 | Пайонир Хай-Бред Интернэшнл, Инк. | Регуляторные элементы растений и способы их применения |
| EP3954202A1 (de) | 2016-07-01 | 2022-02-16 | Pioneer Hi-Bred International, Inc. | Insektizide proteine aus pflanzen und verfahren zu deren verwendung |
| US20210292778A1 (en) | 2016-07-12 | 2021-09-23 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
| MX2019004092A (es) | 2016-10-10 | 2019-09-26 | Monsanto Technology Llc | Proteínas inhibidoras de insectos novedosas. |
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| US11129906B1 (en) | 2016-12-07 | 2021-09-28 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
| BR112019012339A2 (pt) | 2016-12-14 | 2019-11-26 | Pioneer Hi Bred Int | polipeptídeo inseticida recombinante, composição, construto de dna, célula hospedeira, planta transgênica, método para inibir o crescimento ou extermínio de uma praga de inseto ou população de praga, polipeptídeo ipd093 quimérico e proteína de fusão |
| MX2019007491A (es) | 2016-12-22 | 2019-09-06 | Pioneer Hi Bred Int | Proteinas insecticidas y metodos para su uso. |
| BR112019014378A2 (pt) | 2017-01-12 | 2020-02-11 | Pivot Bio, Inc. | Métodos e composições para o aprimoramento de traços de plantas |
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| CN110317242B (zh) * | 2019-06-12 | 2021-03-26 | 南昌大学 | 可特异性结合Cry1Da蛋白的多肽分子及其应用 |
| WO2021076346A1 (en) | 2019-10-18 | 2021-04-22 | Pioneer Hi-Bred International, Inc. | Maize event dp-202216-6 and dp-023211-2 stack |
| WO2021221690A1 (en) | 2020-05-01 | 2021-11-04 | Pivot Bio, Inc. | Modified bacterial strains for improved fixation of nitrogen |
| WO2021222567A2 (en) | 2020-05-01 | 2021-11-04 | Pivot Bio, Inc. | Modified bacterial strains for improved fixation of nitrogen |
| BR112022027035A2 (pt) | 2020-07-14 | 2023-04-11 | Pioneer Hi Bred Int | Proteínas inseticidas e métodos para uso das mesmas |
| BR112023002603A2 (pt) | 2020-08-10 | 2023-04-04 | Pioneer Hi Bred Int | Elementos reguladores de plantas e métodos de uso dos mesmos |
| WO2023278804A1 (en) | 2021-07-02 | 2023-01-05 | Pivot Bio, Inc. | Genetically-engineered bacterial strains for improved fixation of nitrogen |
| WO2023164453A2 (en) * | 2022-02-23 | 2023-08-31 | Pioneer Hi-Bred International, Inc. | Multiple disease resistance genes and genomic stacks thereof |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0400246A1 (de) * | 1989-05-31 | 1990-12-05 | Plant Genetic Systems, N.V. | Verhinderung von Resistenzentwicklung gegen Bt |
| US5188960A (en) * | 1989-06-27 | 1993-02-23 | Mycogen Corporation | Bacillus thuringiensis isolate active against lepidopteran pests, and genes encoding novel lepidopteran-active toxins |
| US5723758A (en) * | 1991-09-13 | 1998-03-03 | Mycogen Corporation | Bacillus thuringiensis genes encoding lepidopteran-active toxins |
| GB9318207D0 (en) * | 1993-09-02 | 1993-10-20 | Sandoz Ltd | Improvements in or relating to organic compounds |
| US5942664A (en) * | 1996-11-27 | 1999-08-24 | Ecogen, Inc. | Bacillus thuringiensis Cry1C compositions toxic to lepidopteran insects and methods for making Cry1C mutants |
| US6218188B1 (en) * | 1997-11-12 | 2001-04-17 | Mycogen Corporation | Plant-optimized genes encoding pesticidal toxins |
| AU7491600A (en) * | 1999-09-15 | 2001-04-17 | Monsanto Technology Llc | Lepidopteran-active bacillus thuringiensis delta-endotoxin compositions and methods of use |
| BRPI0500788A (pt) * | 2004-03-05 | 2006-04-18 | Agrigenetics Inc | combinações de cry1ab e cry1fa como uma ferramenta de controle da resistência a insetos |
| WO2009132850A1 (en) * | 2008-05-01 | 2009-11-05 | Bayer Bioscience N.V. | Armyworm insect resistance management in transgenic plants |
| BRPI1015333A2 (pt) * | 2009-04-17 | 2016-05-24 | Dow Agrosciences Llc | "toxinas cry inseticidas dig-3" |
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| MX2012007126A (es) | 2012-11-12 |
| IL220332A0 (en) | 2012-08-30 |
| IL220332A (en) | 2017-12-31 |
| US20120331590A1 (en) | 2012-12-27 |
| CA2782565A1 (en) | 2011-07-14 |
| WO2011084630A1 (en) | 2011-07-14 |
| BR112012014727A2 (pt) | 2015-08-25 |
| ZA201204925B (en) | 2013-03-27 |
| EP2513317A1 (de) | 2012-10-24 |
| CN102753696A (zh) | 2012-10-24 |
| AU2010339919B2 (en) | 2016-01-21 |
| AR079506A1 (es) | 2012-02-01 |
| JP2013514774A (ja) | 2013-05-02 |
| RU2012129910A (ru) | 2014-01-27 |
| CO6561806A2 (es) | 2012-11-15 |
| EP2513317A4 (de) | 2013-10-02 |
| KR101841296B1 (ko) | 2018-03-22 |
| BR112012014727B1 (pt) | 2019-09-03 |
| AU2010339919A1 (en) | 2012-07-12 |
| CL2012001623A1 (es) | 2013-01-25 |
| NZ601102A (en) | 2014-10-31 |
| KR20120115980A (ko) | 2012-10-19 |
| EP2513317B1 (de) | 2018-01-24 |
| US9499835B2 (en) | 2016-11-22 |
| MX350002B (es) | 2017-08-22 |
| JP2016154536A (ja) | 2016-09-01 |
| RU2590592C2 (ru) | 2016-07-10 |
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