JP5926693B2 - ペプチドの濃度を決定する方法 - Google Patents

ペプチドの濃度を決定する方法 Download PDF

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Publication number
JP5926693B2
JP5926693B2 JP2012556416A JP2012556416A JP5926693B2 JP 5926693 B2 JP5926693 B2 JP 5926693B2 JP 2012556416 A JP2012556416 A JP 2012556416A JP 2012556416 A JP2012556416 A JP 2012556416A JP 5926693 B2 JP5926693 B2 JP 5926693B2
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peptide
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internal
standard
mixture
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Japanese (ja)
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JP2013522174A (ja
JP2013522174A5 (enExample
Inventor
ヨハネス・ツェルヴェック
マイク・シュトコウスキー
ホルガー・ヴェンシュー
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JPT Peptide Technologies GmbH
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JPT Peptide Technologies GmbH
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2/00—Peptides of undefined number of amino acids; Derivatives thereof
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/15—Non-radioactive isotope labels, e.g. for detection by mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2560/00—Chemical aspects of mass spectrometric analysis of biological material

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Genetics & Genomics (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2012556416A 2010-03-12 2011-03-09 ペプチドの濃度を決定する方法 Active JP5926693B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP10002664.0 2010-03-12
EP10002664 2010-03-12
PCT/EP2011/001167 WO2011110341A1 (en) 2010-03-12 2011-03-09 Method for determining the concentration of a peptide

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2016000283A Division JP6186017B2 (ja) 2010-03-12 2016-01-04 ペプチドの濃度を決定する方法

Publications (3)

Publication Number Publication Date
JP2013522174A JP2013522174A (ja) 2013-06-13
JP2013522174A5 JP2013522174A5 (enExample) 2014-04-24
JP5926693B2 true JP5926693B2 (ja) 2016-05-25

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JP2012556416A Active JP5926693B2 (ja) 2010-03-12 2011-03-09 ペプチドの濃度を決定する方法
JP2016000283A Active JP6186017B2 (ja) 2010-03-12 2016-01-04 ペプチドの濃度を決定する方法

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JP2016000283A Active JP6186017B2 (ja) 2010-03-12 2016-01-04 ペプチドの濃度を決定する方法

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US (2) US20130095514A1 (enExample)
EP (1) EP2545384B1 (enExample)
JP (2) JP5926693B2 (enExample)
WO (1) WO2011110341A1 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016032319A1 (en) * 2014-08-28 2016-03-03 Erasmus University Medical Center Rotterdam Protein quantitation with mass spectrometry.
CN107024530B (zh) * 2016-11-25 2018-06-05 北京毅新博创生物科技有限公司 通过内部标准物质谱检测微生物的方法及其产品
WO2019232520A1 (en) * 2018-06-01 2019-12-05 Cerno Bioscience Llc Mass spectral analysis of large molecules
KR102674098B1 (ko) * 2021-01-14 2024-06-12 (주)옵토레인 스위칭 펩티드 및 이를 이용한 멀티플렉스 면역분석 방법

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4027675C2 (de) 1990-08-31 1997-05-07 Biotechnolog Forschung Gmbh Verfahren zur parallelen Herstellung von trägergebundenen oder freien Peptiden, trägergebundene Peptide und ihre Verwendung
US6117635A (en) * 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US7045296B2 (en) * 2001-05-08 2006-05-16 Applera Corporation Process for analyzing protein samples
ATE508361T1 (de) 2001-08-14 2011-05-15 Harvard College Absolute quantifizierung von proteinen und modifizierten formen davon mittels mehrstufiger massenspektrometrie
US20040033530A1 (en) * 2002-04-08 2004-02-19 Awrey Donald E. High throughput purification, characterization and identification of recombinant proteins
US7655433B2 (en) * 2002-06-04 2010-02-02 The Institute For Systems Biology Methods for high-throughput and quantitative proteome analysis
US20060062531A1 (en) * 2004-09-17 2006-03-23 Stephen Turner Fabrication of optical confinements
JP2006300758A (ja) * 2005-04-21 2006-11-02 Apro Life Science Institute Inc 内部標準ペプチドを用いて生体由来試料に含まれる標的タンパク質を定量する方法
DE602005004902T2 (de) * 2005-06-02 2008-10-16 Entelechon Gmbh Künstliches Protein, Verfahren für absolute Quantifizierung der Proteine und seine Benutzung
US8076074B2 (en) * 2005-11-29 2011-12-13 Quest Diagnostics Investments Incorporated Balanced translocation in comparative hybridization
GB0809488D0 (en) * 2008-05-23 2008-07-02 Electrophoretics Ltd Mass spectrometric analysis
EP2124060A1 (en) * 2008-05-23 2009-11-25 ETH Zurich Method for high throughput peptide/protein assay generation and assays generated therewith

Also Published As

Publication number Publication date
US20160223562A1 (en) 2016-08-04
WO2011110341A1 (en) 2011-09-15
EP2545384B1 (en) 2017-12-13
JP2016128812A (ja) 2016-07-14
EP2545384A1 (en) 2013-01-16
JP2013522174A (ja) 2013-06-13
US20130095514A1 (en) 2013-04-18
JP6186017B2 (ja) 2017-08-23

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