JP5903230B2 - Use of liquid animal feed containing animal feed composition containing epilactose - Google Patents

Description

  The present invention relates to an animal feed composition containing epilactose, more specifically, an epidose fed singly or in combination with raw milk, milk substitute, hay, agricultural processing by-products, etc. fed to animals such as cattle, pigs and chickens. The present invention relates to an animal feed composition containing lactose, and an animal feed composition containing the animal feed composition. Furthermore, the present invention relates to the use of the animal feed composition or the animal mixed feed, and more particularly to the use for controlling diarrhea and loose stool of animals and improving the amount of weight gain.

  For livestock farmers such as cattle, pigs, and chickens, it is of great concern to improve productivity by promoting the maintenance of livestock health. For example, diarrhea and dyspepsia occur due to bacterial infection, lack of immunity, etc., and the weight gain of meat for livestock, milk production of dairy cows, and productivity of hen's eggs decrease, and livestock farmers often suffer economically. ing.

  Conventionally, drugs such as antibiotics have been used for the treatment and prevention of diarrhea and the like. However, in recent years, drugs such as antibiotics have been pointed out as adverse effects such as the emergence of multidrug-resistant bacteria and residual in meat, chicken eggs, etc., and consumers are demanding healthy and safe feeding techniques. Therefore, attention has been given to feeding techniques that improve animal productivity by mixing various functional materials such as oligosaccharides and viable fungi into feed (Patent Documents 1 to 4). In order to expand the range of choice of livestock farmers and use such functional materials, it is desired to develop more types of materials.

  On the other hand, in recent years, by the development of a novel epimerase, epilactose (4-O-β-D-galactopyranosyl-D) using lactose (4-O-β-D-galactopyranosyl-D-glucose) as a raw material. -Mannose) can be produced safely and in large quantities (Patent Document 5). Although epilactose was known to exist in trace amounts in milk (Non-patent Document 1), its properties were hardly known. In Patent Document 5, some properties are confirmed, and the use as functional food is being promoted.

JP-A-62-138147 JP 2003-334000 A JP 2004-173620 A Japanese Patent No. 2008-48683 International Publication No. 2008/062555 Pamphlet

T.A. R. I. Cataldi, et al. (1999) Anal. Chem. 71, 4919)

  However, there has been no report on the use of epilactose for animal feed, and it has been unclear whether it can be effectively used for improving the productivity of animals as described above.

  Accordingly, an object of the present invention is to provide an animal feed composition that can effectively use epilactose and can be used to improve animal productivity, and an animal feed composition containing the animal feed composition. . Furthermore, an object of the present invention is to provide a method for suppressing diarrhea and loose stool and improving weight gain of animals using the animal feed composition or animal feed.

The above object is achieved by the following means.
(1) A liquid mixed animal feed containing an animal feed composition containing epilactose is fed to a suckling young animal, and the weight gain of the young animal is improved and the young A method for improving the intake of solid milk in animals .
(2) The method according to the above (1), wherein the target baby-raising animal is a cow.
(3) The method according to (1) or (2) above, wherein no epilactose is added to the solid milk .

  According to the present invention, a novel animal feed composition and animal feed composition containing epilactose can suppress diarrhea and loose stool and improve body weight gain of animals, so that the animals can be healthy. Breeding and producing livestock products safely and efficiently.

It is a figure which shows the influence by epilactose feeding with respect to the number of colon_bacillus | E._coli in the feces of calves.

Hereinafter, embodiments of the present invention will be described in detail.
Epilactose (4-O-β-D-galactopyranosyl-D-mannose) used in the present invention may be of any origin, and the production method and purity are not particularly limited. Since it is used for animal feed, those obtained by the production method of Patent Document 5 that is highly safe and can be produced in large quantities are preferred. That is, an enzyme having 2-epimerase activity for an oligosaccharide having a β-1,4 bond derived from the genus Ruminococcus is reacted with lactose (4-O-β-D-galactopyranosyl-D-glucose). To manufacture. The produced reaction product containing epilactose inactivates the enzyme and may be used as it is without purification, and is purified by known methods such as microfiltration, various chromatographic separations, vacuum concentration, spray drying, freeze drying and the like. It may be used after being purified, powdered, etc. by concentration, drying or the like.

  The epilactose content in the animal feed composition of the present invention is not particularly limited as long as it does not adversely affect the solubility, hygroscopicity, fluidity, storage stability, etc. of the feed composition. In order to more effectively suppress diarrhea and loose stool of animals and improve weight gain, it is preferable to contain 0.01 to 99% by mass based on the mass of the animal feed composition. Epilactose is more preferably 0.1 to 99% by mass, and further preferably 1 to 99% by mass.

  Unless the animal feed composition of the present invention adversely affects the animal's palatability, the solubility of the feed composition, hygroscopicity, fluidity, storage stability, etc., other oligosaccharides, viable bacteria, enzymes, etc. Useful functional materials may be contained.

  For example, as an oligosaccharide (meaning a mixture of 2 to 10 monosaccharides such as glucose, fructose, galactose), isomaltoligosaccharide, nigerooligosaccharide, cordierigosaccharide, trehalose, cyclodextrin, branched cyclodextrin, Cellooligosaccharide, gentio-oligosaccharide, laminari-oligosaccharide, fructooligosaccharide (1-kestose, nystose, fructosyl nystose, etc.), trehalulose, palatinose, palatinose oligosaccharide, glucosyl sucrose, lactosucrose, theandellose, galactooligosaccharide, malto-oligosaccharide, xylooligosaccharide, Oligosaccharides such as chitin oligosaccharides, chitosan oligosaccharides, stachyose, raffinose, levan oligosaccharides, manno-oligosaccharides, inulo-oligosaccharides, agaro-oligosaccharides, neo-agaro-oligosaccharides It is possible to use.

  Examples of viable bacteria include bacteria such as lactic acid bacteria useful as so-called probiotics and yeasts, such as Lactobacillus plantarum, Lactobacillus acidophilus, Lactobacillus salivaius, Lactobacillus reuteri, Lactobacillus myrinas , Lactobacillus pentosus, Lactobacillus gasseri, Lactobacillus fermentum, Lactobacillus galinarum, Lactobacillus rhamnosus, Lactobacillus casei, Enterococcus faecalis, Enterococcus fascium, Bacillus coagulans, Bacillus badius Rikeni Formis, Bacillus cereus, Bacillus subtilis, Clostridium butyricum, Bifidobacterium thermophilus, Bifidobac Lium Shudlongum, Bifidobacterium lactis, Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium longum, Pediococcus asidiractici, Streptococcus bovis, Streptococcus thermo Filus, Streptococcus infantaria, Saccharomyces cerevisiae, and mixtures thereof can be used.

  Examples of enzymes that can be used include cellulase, phytase, β-glucosidase, lipase, amylase, β-glucanase, pentosanase, trypsin, chymotrypsin, protease, peptidase, and mixtures thereof. In addition, antioxidants (citric acid, ascorbic acid, vitamin E, etc.), emulsifiers (sucrose fatty acid ester, lecithin, etc.), thickeners (agar, glucomannan, gelatin, etc.), vitamins (vitamins A, B1, B2) Etc.) and various components such as flavors (herbs, berries, oranges, etc.) can also be mixed.

  In the present invention, animals mean vertebrates other than humans, and include livestock such as cows, horses, pigs, sheep, and chickens, pets such as monkeys, dogs, cats, and small birds, and fish.

  If the target animal which supplies the animal feed composition of this invention is an animal, there will be no restriction | limiting in particular in a kind and feeding time. Preferred target animals are domestic animals such as cows, pigs, horses, sheep, and chickens, which have a high need for improving productivity and are highly effective, and cows are particularly preferred.

  In addition, larvae whose gastrointestinal tract development is not sufficiently matured and diarrhea / loose stool problems and weight gain problems are important are also preferable as target animals, and particularly infants in the suckling stage.

  The animal mixed feed of the present invention is not particularly limited as long as it contains the animal feed composition of the present invention. For example, feed forms such as raw milk, milk replacer, artificial milk, liquid, etc., in the form of a mixed feed to be fed in the required amount of the animal feed composition of the present invention, hay, cereals, food processing by-products, agricultural products It may be in the form of a mixed feed such as powder, pellet, knead, etc., in which a necessary amount of the animal feed composition of the present invention is added to a basic feed such as processed by-products, whole milk powder, and skim milk powder.

  The amount of the animal feed composition of the present invention to be added to the animal feed of the present invention is not particularly limited as long as the effect of suppressing diarrhea / loose stool and improving weight gain of the animal of the present invention is obtained. There is no. Although it depends on the composition of the animal feed composition and the type of animal to be fed, considering the effect and cost, 0.001 to 10% by weight based on the weight of the animal mixed feed, or 1 day, The amount of the animal feed composition added is adjusted so that 0.001 to 100 g, preferably 0.01 to 10 g of the animal feed composition of the present invention is ingested per 1 kg of body weight.

  The method for suppressing diarrhea and loose stool of the animal of the present invention may be performed by feeding the animal feed composition of the present invention or the animal mixed feed to the animal, and the amount of the feed is not particularly limited. In order to more effectively suppress diarrhea and loose stool of animals, preferably, the animal feed composition of the present invention or the animal feed composition of the present invention is used in an amount of 0.1 to 1000 mg per kg body weight of the subject animal per day. Pay to be. In consideration of higher effects and cost, it is preferable to feed 5 to 750 mg of epilactose per kg of the subject animal's body weight per day. Further, it is preferable to feed 10 to 500 mg as epilactose per 1 kg body weight of the subject animal on a day.

  The method for improving the weight gain of an animal of the present invention may be that the animal feed composition or animal mixed feed of the present invention is fed to the animal, and the amount of the feed is not particularly limited. In order to more effectively suppress diarrhea and loose stool of animals, preferably, the animal feed composition of the present invention or the animal feed composition of the present invention is used in an amount of 0.1 to 1000 mg per kg body weight of the subject animal per day. Pay to be. In consideration of higher effects and cost, it is preferable to feed 5 to 750 mg of epilactose per kg of the subject animal's body weight per day. Further, it is preferable to feed 10 to 500 mg as epilactose per 1 kg body weight of the subject animal on a day.

  Feeding of the animal feed composition or animal feed composition of the present invention may be performed by any method, for example, tap water, so that the animal feed composition of the present invention has the above required amount, Mineral-containing water, raw milk, whole milk powder, skimmed milk powder, whey, milk substitute, artificial milk, liquid, liquid feed, etc. may be dissolved or dispersed in a feed or supplied to animals as a drinking liquid. Further, the animal feed composition of the present invention may be supplemented in the form of powder, tablets, pellets, paste bait, etc. so as to have the above-mentioned required amount, and may be fed. You may sprinkle on animal feed, pet food, etc. or mix and feed. It is also preferable to feed the animal mixed feed of the present invention to the above required amount.

  Although the action mechanism of the effect of suppressing animal diarrhea / loose stool and the effect of improving body weight gain by the animal feed composition or the animal feed composition of the present invention is not clear, it is due to prevention of adhesion of pathogenic bacteria to the animal intestinal tract. It is thought to be due to functions such as infection protection, improvement of gut microbiota, palatability improvement, feed efficiency improvement, immune function improvement, lipid metabolism improvement and mineral absorption improvement. In particular, the effect of reducing the number of E. coli in feces, the effect of improving the intake of artificial milk, and the effect of increasing the amount of immunoglobulin A (IgA) in feces, which is an index of intestinal mucosal immunity, have been observed. It is thought to lead to the effect of suppressing loose stool and improving weight gain.

  By controlling animal diarrhea and loose stool and improving body weight gain, animals can be kept healthy, improving productivity and quality of livestock for meat, improving milk production and fatty acid composition of dairy cows, chicken eggs The improvement of productivity can be expected.

Next, the present invention will be specifically described with reference to examples.
1. Test method (1) Test animals Ten introduced Holstein calves were divided into two groups with no statistically biased appearance, body weight, physical condition and the like.
(2) Feed feed Substitute milk: Ushikko Neo (manufactured by Snow Brand Seedling)
Artificial milk: High Pass Food 40 (manufactured by Snow Brand Seedling)
Lukewarm water: approx. 30 ° C
(3) Test Group (Example 1) Five of the test animals were treated with epilactose, and a crude epilactose solution (3.7% by mass of epilactose, 14.7 of lactose) produced by the method of Patent Document 5. 100% / day mixed with milk replacer and fed (replacement milk (following, after dissolution with hot water), lactose content: 0.093 to 0.12% by weight).
(Comparative Example 1) Five of the test animals were used as a control group, and 14.7 g / day of lactose was mixed with the milk substitute and fed.
(4) Feeding method (i) Substitute milk 1 time / day, morning feeding. The sugar material of each test section is mixed with the milk replacer, dissolved in hot water (about 50 ° C) and fed. However, the crude epilactose solution of Example 1 is mixed after being heated to the same temperature as hot water. The feeding was carried out by dissolving 400 g of milk substitute in 2.8 L of hot water on the 0th to 3rd day of feeding and 500 g of milk substitute in 3.5 L of hot water on the 4th to 14th days.
(Ii) Artificial milk Feeded ad libitum (free ingestion). When the remaining food was 50-99 g, it was increased by 100 g from the previous day, and when the remaining food was 0-49 g, it was increased by 200 g from the previous day.
(Iii) Warm water In the morning, it was fed after milk replacer. In the afternoon, I changed my lukewarm water and paid.

2. Content of Evaluation (1) Body Weight The body weight was measured on the first day, the 7th and 14th days of the test for each individual, and the average value of each test section was determined.
(2) Fecal score Each day after the start of the test, the fecal nature of each individual is confirmed until the 14th day, 2 points for diarrhea, 1 point for soft stool, 0 points for normal, and the average value of each test section ( (Per head) was used as the stool score.
(3) Intake of artificial milk The intake of artificial milk that was freely ingested was measured every day, and the total amount of each test section was compared.
(4) Number of Escherichia coli Feces of each individual were collected on the first day of the test, on the 7th and 14th days, and 1 g thereof was used as a test sample. The specimen was diluted 10 times with a sterilized diluent, and a diluted solution having an appropriate concentration was mixed with Chromoaga E. coli (manufactured by Kanto Chemical Co., Inc.) and cultured at 37 ° C. for 24 hours under aerobic conditions. For colonies that had grown in Chromoaga E. coli, the number of bacteria was measured for strains that exhibited typical colony properties.
(5) Amount of immunoglobulin A (IgA) Feces from each individual were collected on the first day and the seventh day of the test, diluted to an appropriate concentration with a diluent, and used as test specimens. The IgA of the specimen was measured by a sandwich ELISA method. The increase rate of the IgA amount from the first day to the seventh day (7th day IgA amount / first day IgA amount) was evaluated.

3. Test results (1) Body weight Table 1 shows the average weight of each test group.

As shown in Table 1, the calf of the epilactose feeding group of Example 1 (epilactose feeding amount: 0.05 to 0.07 g / day / kg-calf) is the calf of the control group of Comparative Example 1 The body weight gain was better than that, and the increase in body weight gain was observed with epilactose.
(2) Fecal score Table 2 shows the fecal score of each test section.

As shown in Table 2, the calf of the epilactose feeding group of Example 1 showed a lower stool score than the calf of the control group of Comparative Example 1 over the whole feeding period, especially at the initial stage until the seventh day. The stool score was low. Therefore, suppression of diarrhea and loose stool was recognized by feeding epilactose.
(3) Artificial milk intake Table 3 shows the total amount of artificial milk intake in each test section.

As shown in Table 3, the calf of the epilactose feeding group of Example 1 had a higher intake of artificial milk than the calf of the control group of Comparative Example 1 throughout the feeding period.
(4) Number of E. coli Changes in the logarithmic value of the number of E. coli in stool from each test area are shown in FIG.

As shown in FIG. 1, the number of E. coli in the feces of calves in the epilactose feeding group of Example 1 was lower than that in the control group of Comparative Example 1.
(5) Amount of immunoglobulin A (IgA) Table 4 shows changes in the amount of IgA in stool of each test section.

  As shown in Table 4, the average increase rate of IgA in the stool of the epilactose feeding group of Example 1 was higher than that of the control group of Comparative Example 1.

  Further, in Example 1, there were three individuals whose IgA amount increase rate exceeded twice, which was significantly higher than 0 in Comparative Example 1. In addition, when the chi-square test was performed in Example 1 and Comparative Example 1 for the number of individuals whose IgA amount increased more than twice, it was significantly high (5%).

  Therefore, improvement of the intestinal mucosal immune system was suggested.

  In addition, this invention is not limited to the structure of said embodiment and Example, A various deformation | transformation is possible within the range of the summary of invention.

  According to the present invention, it is possible to provide an animal feed composition and an animal feed blend that are excellent in safety and efficiency.

Claims (3)

A liquid mixed animal feed containing an animal feed composition containing epilactose is fed to a juvenile at a sucking stage , and the weight gain of the juvenile is improved and the solid of the juvenile is improved. How to improve artificial milk intake . The method according to claim 1, wherein the suckling young animal is a cow . The method according to claim 1, wherein no epilactose is added to the solid artificial milk .

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