JP5895487B2 - Functional film transfer apparatus and cell culture container manufacturing method using the same - Google Patents

Description

  The present invention relates to a transfer device for transferring a functional film into a cell culture vessel and a method for producing a cell culture vessel using the transfer device.

  Patent Document 1 discloses a cell culture support (a cell culture container such as a petri dish) having a surface coated with a layer of a hydrophilic polymer such as a temperature-responsive polymer. By using this cell culture support, it can be easily detached from the cell support and recovered without destroying the cultured / proliferated cells simply by changing the temperature. However, as described in Patent Document 1, it is necessary to perform surface treatment separately by batch treatment on a cell culture container such as a petri dish to provide a functional compound layer. There is still room for improvement.

JP-A-2-21865

  As a technique for solving the inconvenience due to the batch processing of the cell culture support described in Patent Document 1, a raw material in which a large number of functional compound layers are arranged on a strip-shaped release film is prepared in advance, and a labeling technique is used. The functional compound layer is continuously peeled off from the raw material using an adhesive, and each peeled functional compound layer is removed from the original fabric to the cell culture container using an adsorption head that has been used as a conveying means for sheet materials and the like. After transferring, a method of releasing the negative pressure and arranging the functional compound layer at the bottom of the cell culture container can be considered.

  By the way, when a functional film is arranged on the bottom surface of a cell culture container to culture and proliferate cells, if the functional surface of the functional film is damaged, the cells cannot be properly cultured and proliferated. In a conventional general-purpose film transfer device, the raw material is transferred to a guide table via a substantially cylindrical guide roll, and the general purpose film is continuously peeled from the original material by the guide table. For example, if the transport speed of the original web and the rotation speed of the guide roll are deviated, friction may occur between the surface of the original web and the guide roll, and the surface of the original web may be damaged. is there. In particular, it is known that a functional film provided with a hydrophilic polymer is easily damaged by the above-mentioned friction or the like, and how to suppress the functional damage of the functional film is a challenge in the field. Yes.

  The present invention has been made in view of the above circumstances, and a functional film transfer capable of suppressing damage to the functional surface of the functional film when transferring the original fabric provided with the functional film to the guide stand. It is a first problem to provide an apparatus. Moreover, it is set as the 2nd subject to provide the manufacturing method of the cell culture container which arrange | positions a functional film on the bottom face of a cell culture container using the transfer apparatus.

  The apparatus for transferring a functional film according to the present invention guides a raw material including a functional film supported by a release film to a guide table via a guide roll, and the guide table allows a predetermined area of the functional film from the release film. The guide roll is a transfer device that transfers the peeled functional film into the cell culture container, wherein the guide roll is a part of the original fabric until the predetermined region of the functional film is peeled from the peel film. It does not contact the predetermined area of the functional film.

  In the functional film transfer apparatus according to the present invention, since the guide roll does not come into contact with the functional surface of the functional film transferred into the cell culture container, contact between the guide roll and the functional surface of the functional film is prevented. It is possible to suppress damage to the functional surface of the functional film until it is peeled off from the peeling film by the guide stand. Therefore, when the functional film is disposed on the bottom surface of the cell culture container to culture and proliferate cells, the cells can be appropriately cultured and proliferated.

  In one embodiment of the functional film transfer device according to the present invention, the guide roll is not in contact with the functional film side of the original fabric until the predetermined area of the functional film is peeled off from the release film. It is characterized by not touching.

  In the form described above, the guide roll is not provided on the functional film side of the original fabric including the functional film supported by the release film, and the guide roll does not contact the functional film side of the original fabric. Contact between the guide roll and the functional surface of the functional film can be reliably prevented.

  In another embodiment of the functional film transfer device according to the present invention, the guide roll contacts the functional film side of the original fabric until a predetermined area of the functional film is peeled from the peelable film. The guide rolls in contact with the functional film side are not in contact with the predetermined region of the functional film of the original fabric, and at least both ends in the width direction perpendicular to the transport direction of the original fabric It has the roll surface which contact | abuts the said original fabric in a part.

  In the form described above, the functional roll side has a guide roll on the functional film side including the functional film supported by the release film, and the guide roll that comes into contact with the functional film side is peeled off from the release film. Since it has a roll surface that does not come into contact with the original fabric in a predetermined area of the film, it is placed on the roll surface of the guide roll and the predetermined area of the functional film, that is, the bottom surface of the cell culture container, to culture and proliferate cells. It is possible to prevent contact with the area where the functional film is performed, and it is possible to reliably prevent damage to the functional surface of the functional film. Further, since the roll surface is in contact with the original fabric at at least both ends in the width direction orthogonal to the transport direction of the original fabric, the roll surface holds the tension in the width direction of the original fabric. Therefore, it is possible to properly maintain the posture of the raw material transferred to the guide stand.

  The present invention further includes a functional film in which a functional organic compound layer is laminated on one side and a pressure-sensitive adhesive layer is laminated on the other side, and a release film that supports the pressure-sensitive adhesive layer side of the functional film, A step of preparing an original fabric comprising: a step of guiding the original fabric to a guide table via a guide roll so as not to contact a predetermined region of the functional film of the original fabric; and A step of separating the predetermined region of the functional film from the release film to separate the functional film from the original fabric, and a negative pressure adsorption hole for the functional film separated from the original fabric A negative pressure adsorption step on the adsorption surface, a cell culture container is disposed below the functional film, the negative pressure is released, and the functional film is dropped into the cell culture container, through the adhesive layer Functional film It discloses a process for producing a cell culture vessel and having a placing in the cell culture vessel.

  By this invention, it becomes possible to suppress the damage of the functional surface of a functional film.

The schematic diagram explaining an example of the functional film transferred by the functional film transfer apparatus by this invention. The schematic diagram explaining the original fabric which temporarily bonded many functional films to the strip | belt-shaped peeling film. The schematic diagram explaining an example of the functional film transfer apparatus by this invention. The figure explaining an example of a suction head. The figure explaining the state after the fall of a functional film at the time of using the transfer apparatus by this invention. The schematic diagram explaining the other example of the functional film transfer apparatus by this invention. The figure explaining an example of the guide roll used with the other example of the functional film transfer apparatus by this invention. The figure explaining the other example of the guide roll used with the other example of the functional film transfer apparatus by this invention. The figure explaining the further another example of the guide roll used with the other example of the functional film transfer apparatus by this invention. The figure which shows the flask type cell culture container which is an example of the cell culture container which has arrange | positioned the functional film. The figure which shows the manufacture procedure of the flask type cell culture container shown in FIG. The figure which shows the other manufacturing procedure of the flask type cell culture container shown in FIG. The schematic diagram explaining the original fabric which temporarily bonded the functional film arrange | positioned to the flask type cell culture container shown in FIG. 10 to the strip | belt-shaped peeling film.

  Hereinafter, the present invention will be described based on embodiments. FIG. 1 is a cross-sectional view schematically showing an example of a functional film transferred by the functional film transfer apparatus according to the present invention. In the present invention, the target functional film is a film having a pressure-sensitive adhesive layer that is flexible and has a surface provided with a desired function suitable for cell culture. preferable. In a more preferred embodiment, like the functional film 10 shown in FIG. 1, the functional compound layer 12 is laminated on one surface of the film substrate layer 11, and the pressure-sensitive adhesive layer 13 is laminated on the other surface. . In addition, in FIG. 1, 14 is a peeling film which makes the strip | belt shape mentioned later.

<Film base material layer 11>
Although not limited, the film base material layer 11 should just contain the material which can form the said functional compound layer 12 in one surface, and the kind of material is not specifically limited. Typically, as a material for the film base layer 11, polyethylene terephthalate (PET), polystyrene (PS), polycarbonate (PC), triacetyl cellulose (TAC), polyimide (PI), nylon (Ny), low density polyethylene (LDPE), medium density polyethylene (MDPE), vinyl chloride, vinylidene chloride, polyphenylene sulfide, polyether sulfone, polyethylene naphthalate, polypropylene, acrylic and the like.

  The surface of the film base material layer 11 on the side where the functional compound layer 12 is formed can be a surface subjected to an easy adhesion treatment. “Easy adhesion treatment” refers to treatment with an easy adhesive such as polyester, acrylic ester, polyurethane, polyethyleneimine, silane coupling agent, perfluorooctane sulfonic acid (PFOS), and the like.

  The thickness of the film base material layer 11 (when the film base material layer 11 includes an easy adhesion layer in addition to the base material layer, it refers to the entire thickness of the film base material layer including the easy adhesion layer) is particularly Although there is no restriction | limiting, It is preferable that it is the thickness which provides flexibility, for example, is 5 micrometers-400 micrometers, Preferably it is 50 micrometers-250 micrometers.

<Functional compound layer 12>
The functional compound constituting the functional compound layer 12 includes an organic compound or an inorganic compound, and more preferably has a surface that can be changed from cell adhesiveness to cell nonadhesiveness by a predetermined stimulus. Examples thereof include hydrophilic compounds such as a stimulus-responsive polymer and an ethylene glycol chain composed of one or more ethylene glycol units (CH ₂ —CH ₂ —O).

  The film thickness of the functional compound layer is, for example, preferably in the range of 0.5 nm to 300 nm, and more preferably in the range of 1 nm to 100 nm.

  Hereinafter, preferred embodiments of the “stimulus responsive polymer layer” and the “hydrophilic compound layer” will be described.

<Stimulus responsive polymer layer>
The functional organic compound layer is particularly preferably a stimulus-responsive polymer layer. The stimulus-responsive polymer layer is a layer containing a polymer in which the degree of surface cell adhesion is changed by a predetermined stimulus. Examples of the stimulus responsive polymer include a temperature responsive polymer, a pH responsive polymer, an ion responsive polymer, and a photoresponsive polymer. Among these, a temperature-responsive polymer is preferable because it is easy to give a stimulus.

  As the temperature-responsive polymer, for example, a polymer that exhibits cell adhesion at a temperature at which cells are cultured and exhibits cell non-adhesion at a temperature at which the produced cell sheet is peeled may be used. For example, a temperature-responsive polymer has improved affinity to surrounding water at temperatures below the critical dissolution temperature, and the polymer takes up water and swells to make it difficult for cells to adhere to the surface (cell non-adhesiveness). It is preferable to use a material exhibiting a property (cell adhesiveness) that makes the polymer shrink and easily adheres the cell to the surface when water is desorbed from the polymer at a temperature equal to or higher than the same temperature. Such a critical solution temperature is called a lower critical solution temperature. A temperature-responsive polymer having a lower critical solution temperature T of 0 ° C. to 80 ° C., more preferably 0 ° C. to 50 ° C. may be used. This is because the cells can be stably cultured when the lower critical solution temperature T is 0 ° C. to 80 ° C.

  Suitable temperature-responsive polymers include acrylic polymers or methacrylic polymers. Specific examples of suitable temperature-responsive polymers include poly-N-isopropylacrylamide (T = 32 ° C.), poly-Nn-propyl acrylamide (T = 21 ° C.), and poly-Nn-propyl methacrylamide. (T = 32 ° C.), poly-N-ethoxyethyl acrylamide (T = about 35 ° C.), poly-N-tetrahydrofurfuryl acrylamide (T = about 28 ° C.), poly-N-tetrahydrofurfuryl methacrylamide (T = About 35 ° C.), and poly-N, N-diethylacrylamide (T = 32 ° C.).

  As the pH responsive polymer and the ion responsive polymer, those suitable for the cell sheet to be prepared can be appropriately selected.

  The stimulus-responsive polymer layer is prepared by preparing a coating composition containing a monomer that is polymerized to form a target stimulus-responsive polymer and an organic solvent that can dissolve the monomer. The film is coated on the surface of the material to form a coating film, and then the monomer in the coating film is polymerized by an appropriate means such as radiation irradiation to form a polymer. And a grafting reaction between them.

<Hydrophilic compound layer>
As another embodiment of the functional organic compound layer, hydrophilicity such as an ethylene glycol chain composed of one or more ethylene glycol units (an ethylene glycol chain composed of a plurality of ethylene glycol units can be referred to as a “polyethylene glycol chain”). A layer of a functional compound.

  The terminal of the ethylene glycol chain may be in a form blocked with a hydroxyl group, or the terminal of the ethylene glycol chain may be in a form in which another substance such as a biological substance is linked by a covalent bond.

  A layer containing an ethylene glycol chain whose end is blocked with a hydroxyl group can provide a hydrophilic surface to which cells are difficult to adhere.

  Bio-related substances that can be covalently bonded to the end of ethylene glycol chain include antigens, antibodies, DNA, RNA, peptides, hormones, enzymes, cytokines, sugar chains, lipids, coenzymes, enzyme inhibitors, cells, and other functions. The protein which has is included. Furthermore, the low molecular weight compound which has affinity with such a biological substance, and a high molecular compound are also contained in the range of a biological substance.

  In order to immobilize a hydrophilic compound layer such as an ethylene glycol chain on the surface of a resin film base layer, it can be physically adsorbed on the surface of the film base layer in advance. A primer layer containing a polysiloxane containing a functional group in a side chain that can form a covalent bond by reacting with a hydroxyl group at the end of the chain is provided. The functional group on the side chain of the polysiloxane is preferably a glycidyl group or an epoxy group. The primer layer can be formed by applying a silanol compound having a desired side chain to the surface of the film substrate layer, and performing condensation polymerization on the surface to convert it into polysiloxane.

  Next, the functional group of the primer layer and the hydroxyl group of polyethylene glycol in which two or more ethylene glycol or ethylene glycol units are repeated are reacted to form a covalent bond, thereby immobilizing the ethylene glycol chain. At this time, ethylene glycol or polyethylene glycol containing a catalytic amount of concentrated sulfuric acid is brought into contact with the primer layer.

  A layer containing ethylene glycol chains whose ends are blocked with hydroxyl groups is formed in this way.

  Furthermore, if necessary, at least one functional group capable of forming a covalent bond with another substance is directly or indirectly linked to one end of the ethylene glycol chain. The method for introducing the functional group is not particularly limited.

<Adhesive layer 13>
Examples of the pressure-sensitive adhesive constituting the pressure-sensitive adhesive layer 13 include polyester resin, acrylic ester resin, polyurethane resin, polyethyleneimine resin, silane coupling agent, and perfluorooctane sulfonic acid (PFOS). Among them, acrylic ester Resins, polyurethane resins and the like can be preferably used.

  Although the thickness of an adhesive layer is not specifically limited, It is preferable that they are 10 micrometers-300 micrometers, and it is more preferable that they are 20 micrometers-200 micrometers.

  The functional film 10 having the layer structure shown in FIG. 1 can be produced by an arbitrary method. In the present embodiment, the functional film 10 is produced as follows. That is, the pressure-sensitive adhesive layer 13 is applied to the entire surface of the strip-shaped release film 14, and the strip-shaped film base material layer 11 and the functional compound layer 12 having the same width are laminated thereon. As a result, as shown in the drawing, a long object 20 (see FIG. 2) is formed, which is composed of a four-layer structure of the release film 14, the pressure-sensitive adhesive layer 13, the film base material layer 11, and the functional compound layer 12. . The release film 14 is not particularly limited as long as it has the required strength and flexibility. For example, a release treatment such as a silicone release agent is applied to a film made of a resin such as polyethylene terephthalate or a foamed film thereof. Can be mentioned.

  A form (not shown) having an outer shape of the functional film 10 to be obtained is pushed down from the surface opposite to the release film 14 of the long object 20 so as to reach the surface of the release film 14. Thereby, in the functional compound layer 12, the film base layer 11, and the pressure-sensitive adhesive layer 13, a cut line 21 that forms the outer shape of the functional film 10 is formed. By performing the stamping operation on the long object 20 continuously at a predetermined interval, a long object 20 having a plurality of cut lines 21 as shown in FIG. 2 is obtained. The long object 20 is wound into a roll so that the functional film 10 side is on the inside, and is made into a raw fabric 22 (see FIG. 3) and stored.

  In addition, there is no restriction | limiting in the shape in planar view of the functional film 10, It can take arbitrary shapes, The formwork according to it is used. In the example shown in FIG. 2, the functional film 10 is substantially circular in plan view, but the functional film 10 that is planned to be disposed on the bottom surface 51A of the cell culture vessel 50A having a tapered shape as shown in FIG. In this case, the score line 21 has substantially the same shape as the shape of the inner peripheral contour of the bottom surface 51A of the cell culture vessel 50A (see FIG. 13).

  The long object 20 is wound into a roll so that the functional film 10 side is on the inside, and is made into a raw fabric 22 (see FIG. 3) and stored.

  FIG. 3 shows an example of a functional film transfer device according to the present invention. The functional film transfer device 30 shown in the figure includes an air cylinder 31 attached to a conventionally known three-dimensional robot arm (not shown), and an adsorption as shown in FIG. A head 40 is detachably mounted. The air cylinder 31 and the suction head 40 are movable in the XY axis direction which is the surface direction of the suction surface 41 and the Z axis direction which is the vertical direction by a three-dimensional robot arm control device (not shown). .

  As shown in FIG. 3, a guide base 32 extending in the horizontal direction or in a slightly inclined downward direction is located in the vicinity of the air cylinder 31, and the long object 20 unwound from the original fabric 22. Is guided along the guide roll 33, moves along the upper surface of the guide table 32, makes a U-turn at the tip of the guide table 32, and is then wound up by the take-up roll 34. When the long object 20 makes a U-turn at the tip of the guide base 32, the inner area defined by the cut line 21 is peeled off from the release film 14 supporting the pressure-sensitive adhesive layer 13 side of the functional film 10, and guided. It is sent out in the direction of the extended line on the upper surface of the table 32. That is, the functional film 10 having a predetermined shape composed of a three-layer structure of the functional compound layer 12, the film base layer 11 and the pressure-sensitive adhesive layer 13 has the functional compound layer 12 as the upper surface side and the pressure-sensitive adhesive layer 13 side as the lower surface. The film is continuously peeled off from the release film 14 in a posture set to the side and sent out in a horizontal direction or a slightly inclined direction.

  The functional film transfer device 30 further includes cell culture container support means 35 that can be repeatedly moved and stopped intermittently with the cell culture container 50 placed in a horizontal posture.

  Preferably, an air nozzle 37 having a lateral width substantially equal to the lateral width of the functional film 10 is directed obliquely upward in the vicinity of the front end of the guide table 32 and below the functional film 10 to be delivered. It can be installed in a different posture. By receiving the air jet from the air nozzle 37 from below, the functional film 10 peeled from the release film 14 and sent out can maintain a substantially horizontal posture thereafter.

  4A and 4B, the suction head 40 includes a suction surface 41 that is a substantially flat surface and an air chamber on the back side of the suction surface 41, as shown in a sectional view and a plan view viewed from below. 42, and the air chamber 42 is connected to the air passage 36 of the air cylinder 31. The air passage 36 is connected to appropriate air suction and exhaust means (not shown). In the illustrated example, the shape of the suction surface 41 is circular in plan view, and a number of negative pressure suction holes 44 are formed in the suction surface 41.

  Circular cut lines 21 having substantially the same size as the suction surface 41 are formed at regular intervals on the original fabric 22 used in the functional film transfer device 30 provided with the suction head 40. As described above, the adhesive film 10 is continuously peeled from the release film 14 in a posture in which the pressure-sensitive adhesive layer 13 side is the lower surface side, and is sent out in a horizontal direction or a slightly inclined downward direction.

  The operation of the functional film transfer device 30 will be described. First, the raw fabric 22 is guided through the guide roll 33 so as to pass through the upper surface of the guide table 32 and its tip, and the guide table. The original fabric 22 after making a U-turn at the tip of 32 is wound on a winding roll 34. In this state, when the raw fabric 22 is wound up, as described above, the functional film 10 peeled from the release film 14 from the front end of the guide stand 32 is in a posture with the adhesive layer 13 side as the lower surface side. It is sent out in a horizontal direction or a slightly inclined downward direction at regular intervals.

  Here, in the example shown in FIG. 3, the guide rolls on the functional compound layer 12 side of the functional film 10 of the long object 20 (raw fabric 22) until a predetermined region of the functional film 10 is peeled from the release film 14. Is not provided. That is, the guide roll 33 is on the functional compound layer 12 side of the functional film 10 of the original fabric 22 until the inner region defined by the cut line 21 of the functional film 10 is peeled from the release film 14. The guide rolls 33 for guiding the original fabric 22 to the guide table 32 are all provided on the peeling film 14 side of the long article 20 (original fabric 22). Thereby, since the functional compound layer 12 of the functional film 10 does not contact a guide roll, damage to the functional compound layer 12 side of the functional film 10 can be suppressed. In the illustrated example, the number of guide rolls 33 for guiding the original fabric 22 to the guide table 32 is one, but a plurality of guide rolls 33 can be combined to guide the original fabric 22 in the direction of the guide table 32.

  Then, a robot arm control device (not shown) positions the air cylinder 31 and the suction head 40 at a position where the delivered functional film 10 can be sucked by the suction head 40 with negative pressure. At this time, in order to maintain the posture of the functional film 10 until the negative pressure is adsorbed to the adsorption surface 41, the suction means while jetting gas such as air or nitrogen from the air nozzle 37 to the functional film 10. As a result, the air in the air chamber 42 is sucked to generate a negative pressure on the suction surface 41, whereby the functional film 10 is sucked to the suction surface 41 with a negative pressure. In order to prevent the original fabric 22 on the guide table 32 from being lifted by the pressure of the gas from the air nozzle 37 and the suction force from the suction head 40, a small distance from the original fabric 22 is placed on the upper surface side of the guide table 32 and in the vicinity of the tip. A plate 38 is provided.

  Then, in a state where the functional film 10 is attracted to the suction surface 41 by negative pressure, the control device operates the robot arm, and the air cylinder 31 and the suction head 40 are supported and conveyed by the cell culture vessel support means 35. Move to a position directly above the cell culture vessel 50. That is, the cell culture container 50 is disposed below the functional film 10. After the movement, the air cylinder 31 and the suction head 40 are lowered until the distance between the bottom surface 51 which is the functional film receiving surface of the cell culture container 50 and the functional film 10 is about 0.1 mm to 10 mm, and negative at the lowered position. Release pressure. Here, the exhaust means of the air cylinder 31 is operated to send a gas such as air or nitrogen having a predetermined pressure into the air chamber 42, and this gas is discharged from each negative pressure adsorption hole 44 formed in the adsorption surface 41 (exhaust gas). )

  When the negative pressure is released at the lowered position as described above, the suction force of the adsorption surface 41 disappears, so that the functional film 10 adsorbed on the adsorption surface 41 is directed toward the bottom surface 51 of the cell culture vessel 50 by its own weight. Fall. In the above example, the adsorption surface 41 of the adsorption head 40 and the bottom surface 51 of the cell culture vessel 50 supported on the cell culture vessel support means 35 are both substantially horizontal and parallel. FIG. 5 shows a state after dropping in which the back surface of the functional film 10, that is, the entire surface on the pressure-sensitive adhesive layer 13 side is in contact with the bottom surface 51 of the cell culture vessel 50, and the functional film 10 passes through the pressure-sensitive adhesive layer 13. The cell culture vessel 50 is fixedly disposed on the bottom surface 51.

  FIG. 6 shows another example of the functional film transfer device according to the present invention. The functional film transfer device 30A shown in the figure is a long object 20 (raw fabric 22) until the predetermined area of the functional film 10 is peeled from the release film 14 with respect to the functional film transfer device 30 shown in FIG. Guide rolls 33Aa, 33Ab, 33Ad are provided on the functional compound layer 12 side of the functional film 10, and the rolls of the guide rolls 33Aa, 33Ab, 33Ad arranged on the functional compound layer 12 side of the functional film 10 are provided. The surface is in contact with the functional compound layer 12 side of the functional film 10 that is peeled off from the release film 14 by the guide table 32, but the inner side of the functional compound layer 12 side defined by the cut line 21. It does not come into contact with the region.

  Hereinafter, among the guide rolls 33Aa, 33Ab, and 33Ad, the form of the guide roll 33Aa close to the guide base 32 will be described as an example.

  As shown in FIG. 7, in this long object 20, an area (predetermined area peeled off from the release film 14) E1 defined by the cut line 21 is the center in the width direction orthogonal to the transfer direction of the long object 20. A region E <b> 2 where the functional film 10 is not peeled from the release film 14 of the long object 20 is provided at both ends in the width direction.

  The guide roll 33Aa has a roll surface 39Aa that comes into contact with the long object 20 at a position corresponding to the region E2, that is, a position that comes into contact with both ends of the long object 20 in the width direction. There is no roll surface at a position corresponding to the central portion in the width direction.

  Thereby, since the functional compound layer 12 side of the functional film 10 peeled from the peeling film 14 and disposed on the bottom surface 51 of the cell culture vessel 50 does not come into contact with the roll surface 39Aa of the guide roll 33Aa, Damage to the functional compound layer 12 side of the functional film 10 disposed on the bottom surface 51 of the cell culture container 50 can be reliably suppressed. In addition, since the roll surface 39Aa of the guide roll 33Aa is provided at a position where the roll surface 39Aa comes into contact with both ends of the long object 20 in the width direction, the tension in the width direction of the long object 20 can be secured, and the guide base 32 is provided. The posture of the long object 20 until it is guided to can be properly maintained.

  FIG. 8 shows another example of a guide roll used in another example of the functional film transfer device according to the present invention. In this long object 20, areas (predetermined areas peeled from the release film 14) defined by the cut lines 21 are arranged in two rows in the width direction orthogonal to the transfer direction of the long object 20 and are continuous in the transfer direction. The region E <b> 2 where the functional film 10 is not peeled from the peeling film 14 of the long object 20 is provided at the center and both ends in the width direction.

  The guide roll 33Aa has roll surfaces 39Aa and 39Ab that come into contact with the long object 20 at positions that come into contact with both ends and the center of the long object 20 in the width direction.

  Thereby, the functional compound layer 12 side of the functional film 10 disposed on the bottom surface 51 of the cell culture container 50 does not contact the roll surfaces 39Aa and 39Ab of the guide roll 33Aa, and at the same time, the long object 20 and the guide roll. Since the contact area with the roll surfaces 39Aa and 39Ab of 33Aa can be increased, the shift of the relative feed amount between the long object 20 and the guide roll 33Aa can be suppressed, and the width of the long object 20 can be suppressed. The posture of the long object 20 can be more appropriately maintained by suppressing the displacement of the direction.

  In addition, the shape and size of the cell culture container may be specified in advance, and the shape and size of the functional film placed in the cell culture container are determined by the shape and size of the container. It is done. On the other hand, there is a case where the width of the original film of the functional film cannot be set freely. Even if the predetermined regions are efficiently arranged in parallel along the transfer direction of the original film, {(the width of the original film) − Since a band-like surplus area may exist as an excess of (an integral multiple of the width of the predetermined area)}, it is preferable to bring the roll surface of the guide roll into contact with the surplus area.

  FIG. 9 shows still another example of the guide roll used in another example of the functional film transfer apparatus according to the present invention. In the long object 20, similarly to the example shown in FIG. 7, the area defined by the cut line 21 (predetermined area peeled from the release film 14) E1 is in the width direction orthogonal to the transfer direction of the long object 20. A region E <b> 2 where the functional film 10 is not peeled from the release film 14 of the long object 20 is provided at both ends in the width direction.

  Here, the region E1 defined by the cut line 21 is continuously formed at a predetermined interval in the transfer direction, and is adjacent in the central portion in the width direction orthogonal to the transfer direction of the long object 20. A region E2 where the functional film 10 is not peeled from the release film 14 is present between the regions E1.

  Therefore, the guide roll 33Aa has a roll surface 39Aa that contacts the long object 20 at a position that contacts both ends in the width direction of the long object 20, and has a bridging surface 39Ac that connects the roll surfaces 39Aa. By appropriately setting the arrangement interval of the region E1 and the arrangement interval of the bridging surface 39Ac, the raw fabric 22 and the bridging surface 39Ac of the guide roll 33Aa can be brought into contact with each other between the adjacent regions E1, and cell culture is performed. While preventing contact between the functional compound layer 12 side of the functional film 10 disposed on the bottom surface 51 of the container 50 and the roll surfaces 39Aa, 39Ac of the guide roll 33Aa, the long object 20 and the roll surface 39Aa of the guide roll 33Aa , 39Ac can be increased. Moreover, the rigidity of the guide roll 33Aa can be effectively increased by connecting the roll surfaces 39Aa with the bridging surface 39Ac.

  In addition, the hole diameter, base number, and shape of the negative pressure adsorption hole 44 shown in FIG. 4B can be selected as appropriate. Although not shown, for example, the hole diameter in the vicinity of the center of the suction surface 41 can be increased or the number of holes can be increased, and as another example, the negative pressure suction hole 44 is formed by a plurality of parallel slits. You can also

  Further, the shape of the suction surface 41 in the suction head 40 is not limited to the circular shape described above, but is determined depending on the shape of the bottom surface 51 of the cell culture container 50 that transfers the functional film 10 that has been adsorbed. Further, as the cell culture container 50, in addition to a dish-shaped or bowl-shaped container having an open top, a flask-shaped container 50A as shown in FIG.

  As shown in FIG. 10A, the flask-type cell culture container 50A includes a container part 100 and a lid 110. The container unit 100 includes at least a bottom surface 51A, a side wall portion 102 erected on the periphery of the bottom surface 51A, and a top surface portion 103 that is joined to the upper end portion of the side wall portion 102 and is opposed to the bottom surface 51A. The bottom surface 51A has a shape in which one end side of a rectangular flat plate is narrowed, and a through hole 104 is formed in the side wall portion 102 corresponding to the narrowed tip. And the neck part 105 extended to the outer side of the container part 100 from the periphery of the through-hole 104 is provided, and the lid | cover 110 is mounted | worn detachably there. By combining the container part 100 and the lid 110, a flask-type cell culture container 50A is formed.

  FIG.10 (b) shows the cross section which follows the bb line of Fig.10 (a), FIG.10 (c) shows the cross section which follows the cc line. In the internal space surrounded by the bottom surface 51 </ b> A, the side wall portion 102, and the top surface portion 103 of the container portion 100, an inner chamber 130 for containing cells and a culture medium is formed. The functional film 10 described above is fixed to a part of the bottom surface 51 </ b> A facing the inner chamber 130.

  The functional film 10 can be fixed when the surface to which the functional film 10 is fixed is not opened and is located in a closed container like the flask type cell culture container 50A. What is necessary is just to complete the target cell culture container by fixing the functional film 10 to a shape-shaped member, and combining the member after film fixation with another member.

  For example, as shown in FIG. 11, a first member 201 having a bottom surface 51A and a side wall portion 102, the side opposite to the fixing surface of the functional film 10 on the bottom surface 51A being opened, and the top surface portion 103 on the opened surface. The container part 100 is formed by joining the 2nd member 202 corresponding to these. At this time, before joining the second member 202, the functional film 10 is fixed to the inner surface of the bottom surface 51A of the first member 201 using the functional film transfer device 30 according to the present invention. The first member 201 and the second member 202 are joined in a liquid-tight manner by an appropriate method so that the culture solution does not leak out according to the purpose of cell culture, if necessary.

  In the embodiment shown in FIG. 12, the first member 301 corresponding to the bottom surface 51 </ b> A, the second member 302 corresponding to the side wall portion 102 including the neck portion 105, and the third member 303 corresponding to the top surface portion 103 are joined. Thereby, the container part 100 is formed. In this aspect, the functional film 10 is fixed to the portion of the first member 301 corresponding to the inner surface of the bottom surface 51A by the method described above.

  When the functional film 10 is transferred to the flask-type cell culture vessel 50A having the shape of the bottom surface 51A shown in FIGS. 10 to 12, the one end side is narrow as shown in FIG. While using the long object 20 with the cut line 21 having the formed shape, the shape of the suction surface 41 of the suction head 40 is also a shape corresponding thereto.

  In this invention, the material which comprises the cell culture container 50 is not specifically limited, The material generally used in cell culture can be used. For example, polystyrene resin, polyester resin, polyethylene resin, polyethylene terephthalate resin, polypropylene resin, ABS resin, nylon, acrylic resin, fluorine resin, polycarbonate resin, polyurethane resin, methylpentene resin, phenol resin, melamine resin, epoxy resin, vinyl chloride A resin material such as a resin, a resin material containing at least one of the above-described surface hydrophilized treatment, and an inorganic material such as glass or quartz are preferable, but a resin material is preferable. The resin material is preferably a polystyrene resin or a polyethylene terephthalate resin.

  As described above, according to the present invention, it is possible to provide a cell culture container in which damage to the functional compound layer is suppressed.

10 ... functional film,
11 ... film base material layer,
12 ... Functional compound layer,
13 ... adhesive layer,
14 ... stripping release film,
20 ... long object composed of four layers,
21 ... a score line forming the outer shape of the functional film,
22 ... roll-shaped original fabric,
30, 30A ... transfer device,
31 ... Air cylinder,
32 ... Information desk,
33, 33A (33Aa-33Af) ... guide roll,
34 ... take-up roll,
35 ... Cell culture vessel support means,
36 ... Airway,
37 ... Air nozzle,
38 ... Presser plate,
39Aa, 39Ab, 39Ac ... roll surface,
40: Suction head,
41 ... adsorption surface,
42 ... Air chamber,
44 ... negative pressure adsorption hole,
50, 50A ... cell culture vessel,
51, 51A ... bottom of cell culture vessel

Claims (4)

The raw containing a functional film that is supported by the release film was guided to a guide table through a guide roll, peeling off the predetermined area of the functional film from the release film at the base guiding, negative peeled functional film Transfer that adsorbs negative pressure to the adsorption surface with pressure adsorption holes, releases the negative pressure, drops the functional film onto the cell culture container disposed below the functional film, and transfers the functional film into the cell culture container A device,
The guide roll is not in contact with the predetermined area of the functional film of the original fabric until the predetermined area of the functional film is peeled off from the release film. .   2. The functional film according to claim 1, wherein the guide roll does not contact the functional film side of the original fabric until a predetermined region of the functional film is peeled from the release film. Transfer device. The guide roll is in contact with the functional film side of the original fabric until the predetermined area of the functional film is peeled off from the release film,
The guide roll that contacts the functional film side does not contact the predetermined region of the functional film of the original fabric, and at least at both ends in the width direction orthogonal to the transport direction of the original fabric The functional film transfer device according to claim 1, further comprising a roll surface that comes into contact with the original fabric. A raw fabric comprising a functional film having a functional organic compound layer laminated on one side and a pressure-sensitive adhesive layer laminated on the other side, and a release film supporting the pressure-sensitive adhesive layer side of the functional film. A process to prepare;
Guiding the original fabric to a guide table via a guide roll so as not to come into contact with a predetermined area of the functional film of the original fabric;
Separating the functional film from the original fabric by peeling the predetermined region of the functional film from the release film at the guide stand; and
A step of negatively adsorbing the functional film separated from the original fabric on an adsorption surface having negative pressure adsorption holes;
A cell culture container is disposed below the functional film, the negative pressure is released, the functional film is dropped onto the cell culture container, and the functional film is placed on the cell culture container via the adhesive layer. And a step of arranging the cell culture container.

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