JP5884430B2 - Functional film transfer device and cell culture container manufacturing method - Google Patents

Description

  The present invention relates to a transfer device for negatively adsorbing a functional film to a negative pressure adsorption head and a method for producing a cell culture container using the same.

  Patent Document 1 discloses a cell culture support (a cell culture container such as a petri dish) having a surface coated with a layer of a hydrophilic polymer (temperature-responsive polymer) that is cultured. By using this cell culture support, it can be easily detached from the cell support and recovered without destroying the cultured / proliferated cells simply by changing the temperature. However, as described in Patent Document 1, it is necessary to perform surface treatment separately by batch treatment on a cell culture container such as a petri dish to provide a functional compound layer. There is still room for improvement.

JP-A-2-21865

  As an improved method for this purpose, an original fabric in which a large number of functional compound layers are arranged on a strip-shaped release film is prepared in advance, and the functional compound layer is continuously formed from the original fabric using a labeling method. After peeling, each peeled functional compound layer is transferred to the cell culture container using a negative pressure adsorption head that has been used as a means for transporting sheet materials, etc. A method of evacuating the head and arranging the functional compound layer at the bottom of the cell culture container is conceivable.

  When a functional film is placed on the bottom surface of a cell culture container to culture and proliferate cells, both are in close contact with each other with no air bubbles between the bottom surface of the cell culture container and the back surface of the functional film. Is required. For this reason, the functional compound layer peeled from the raw fabric is placed at an appropriate position on the adsorption surface of the negative pressure adsorption head, and the functional film is adsorbed on the adsorption surface of the negative pressure adsorption head to the top of the cell culture vessel. It is necessary to carry out a process of transferring the functional film to an appropriate position on the bottom surface of the cell culture container by removing the negative pressure and exhausting it.

  However, in the conventional functional film transfer device, the functional film is negatively adsorbed on the adsorption surface of the negative pressure adsorption head by using the suction force of the negative pressure adsorption head. When the sheet is sent out of an appropriate position with respect to the pressure adsorption head, the position deviation cannot be corrected sufficiently, and the functional film is not disposed at an appropriate position on the adsorption surface of the negative pressure adsorption head. there is a possibility. If the functional film is not arranged at the proper position on the suction surface of the negative pressure adsorption head in this way, the negative pressure of the negative pressure adsorption head is eliminated and the negative pressure adsorption head is exhausted, and the functional film is removed from the cell culture container. When placing at the bottom of the functional film can not be placed at the appropriate position of the bottom of the cell culture container, for example, there are bubbles between the bottom of the cell culture container and the back of the functional film, There is a possibility that the cells cannot be cultured or proliferated precisely.

  The present invention has been made in view of the above circumstances, and the functional film peeled off from the release film is negatively adsorbed on a negative pressure adsorption head using a transfer device, and the functional film is attached to the negative pressure adsorption head. When transferring from the initial position to the cell culture container, place the functional film at the appropriate position on the adsorption surface of the negative pressure adsorption head and drop the functional film onto the appropriate position on the bottom surface of the cell culture container It is a first object to provide a transfer device that enables the above. Moreover, it is set as the 2nd subject to provide the manufacturing method of the cell culture container which arrange | positions a functional film on the bottom face of a cell culture container using the transfer apparatus.

  The inventors of the present invention conducted a number of experiments to solve the above problem, and in the transfer device known in the art, as shown in FIG. 11 (a), a plurality of negative pressure adsorption holes having the same hole diameter. In some cases, the functional film 3 is disposed at a position shifted on the suction surface 1 of the negative pressure suction head 7 having 2 and when exhausted from the negative film after the negative pressure is released, As shown in FIG. 11B, the functional film 3 is substantially parallel to the bottom surface 5 of the cell culture vessel 4 as a result of the action of substantially equal exhaust pressure and equal amount of air from each negative pressure adsorption hole 2. In a state where the posture is maintained, the cell culture container 4 falls toward the bottom surface 5 of the cell culture container 4, and one end of the functional film 3 is in contact with the side wall portion 8 of the cell culture container 4 as shown in FIG. Arranged on the bottom surface 5 of the cell culture vessel 4, there is a bubble 6 between them It was finding the would.

  The present invention is based on the above knowledge, and the transfer device according to the present invention is configured such that the functional film supported by the release film is peeled from the release film, and the negative pressure is applied to the suction surface provided with the negative pressure suction holes. A transfer device for adsorption, the transfer device comprising an air nozzle that jets gas to the lower surface of the functional film peeled from the release film, and the gas that has been blown from the air nozzle The functional film is adsorbed at a negative pressure on the adsorption surface.

  When the functional film peeled from the peeled film using the transfer device according to the present invention is negatively adsorbed to the suction surface having the negative pressure suction holes, the suction force in the negative pressure suction holes and the gas ejected from the air nozzle Because the functional film can be negatively adsorbed to the adsorption surface using the pressure of the pressure, the posture of the functional film can be properly maintained until it is separated from the release film and adsorbed to the adsorption surface by negative pressure At the same time, the functional film peeled from the release film can be quickly placed at an appropriate position on the adsorption surface. Even when the functional film is sent to a position shifted from the suction surface, the function for the suction surface can be adjusted by adjusting the suction force in the negative pressure suction hole and the pressure of the gas ejected from the air nozzle. The positional shift of the functional film can be corrected, and the functional film can be disposed at an appropriate position on the suction surface.

  As a result, the functional film is transferred to the cell culture vessel from the initial position in a state where the functional film is negatively adsorbed on the adsorption surface using the negative pressure adsorption head, and the negative pressure is released and simultaneously from each negative pressure adsorption hole of the negative pressure adsorption head. When evacuating and separating the functional film from the negative pressure adsorption head on the cell culture container, it is not necessary to adjust the position of the cell culture container or the negative pressure adsorption head. Drop toward the proper position of the culture vessel. Therefore, since the lower surface of the functional film and the bottom surface of the cell culture container are disposed flush with each other, the entire functional film is disposed on the bottom surface of the cell culture container with no air bubbles therebetween.

  In addition, as a gas ejected with respect to the lower surface of a functional film in order to maintain the attitude | position of a functional film, air and nitrogen can be used, for example.

  In a preferred form of the transfer device according to the present invention, the transfer device includes a plurality of the air nozzles. In this way, the transfer device includes a plurality of air nozzles, so that gas can be ejected from multiple directions to the functional film until it is peeled off from the peeled film and adsorbed to the suction surface by negative pressure. The posture can be maintained more appropriately. Moreover, since the flow volume of the gas ejected from each air nozzle can be suppressed, the configuration of the air nozzle can be simplified. Furthermore, even when the functional film is sent to a position shifted from the suction surface, the functional film can be corrected in multiple directions with respect to the suction surface. It is possible to accurately arrange at an appropriate position.

  In a preferred form of the transfer device according to the present invention, the transfer device may include at least two air nozzles in the feeding direction of the functional film when the functional film is suctioned to the suction surface by negative pressure. Alternatively, at least two air nozzles may be provided in a direction orthogonal to the feeding direction of the functional film when the functional film is adsorbed to the adsorption surface by negative pressure.

  If the transfer device has at least two air nozzles in the feeding direction of the functional film when the functional film is adsorbed to the suction surface by negative pressure, the pressure of the gas ejected from the air nozzle can be set appropriately. The position of the functional film in the feeding direction of the functional film can be adjusted. Further, if the transfer device has at least two air nozzles in a direction orthogonal to the feeding direction of the functional film when the functional film is adsorbed to the suction surface by negative pressure, the pressure of the gas ejected from the air nozzle is reduced. By appropriately setting, the position of the functional film in the direction orthogonal to the feeding direction of the functional film can be adjusted.

  In a preferred form of the transfer device according to the present invention, the transfer device includes an adjusting means for adjusting the flow rate and / or pressure of the gas ejected from the air nozzle. By providing the adjusting means for adjusting the flow rate and / or pressure of the gas ejected from the air nozzle in this way, for example, a functional film until it is peeled off from the peeling film by an appropriate detecting means and is sucked to the suction surface by negative pressure. If the functional film deviates from the proper position, the flow rate and pressure of the gas ejected from the air nozzle are adjusted based on the detected positional information, and the functional film is displaced. And the functional film can be guided to an appropriate position on the suction surface. Thereby, while being able to arrange | position a functional film accurately in the suitable position of an adsorption | suction surface, the base of the air nozzle to be used can be suppressed and the rise in the manufacturing cost of a transfer apparatus can be suppressed.

  In the present invention, the shape of the suction surface of the negative pressure adsorption head for negatively adsorbing the functional film is not particularly limited, but preferably the shape of the bottom surface of the cell culture container containing the functional film to be transferred Matched shape. In addition, the flow rate and pressure of the gas ejected from the air nozzle are not particularly limited, and should be set experimentally to optimum values in consideration of the physical properties of the actual functional film and the bottom shape of the cell culture vessel. That's fine.

  The present invention further includes a functional film in which a functional organic compound layer is laminated on one side and a pressure-sensitive adhesive layer is laminated on the other side, and a release film that supports the pressure-sensitive adhesive layer side of the functional film, A step of preparing an original fabric comprising, a step of transferring the original fabric in a posture with the functional organic compound layer of the functional film as an upper surface side, and peeling the functional film from the release film. A step of separating the functional film from the original fabric, and a gas is ejected to the lower surface of the functional film separated from the original fabric, and the functional film which has received the gas ejection is provided with a negative pressure adsorption hole. A negative pressure adsorption step on the adsorption surface provided, a cell culture container is disposed below the functional film, the negative pressure is eliminated, the functional film is dropped into the cell culture container, and the adhesive layer is Through the machine Discloses placing a sex film into the cell culture container, a manufacturing method of a cell culture vessel, characterized in that it consists.

  By using the transfer device according to the present invention, the functional film can be negatively adsorbed at an appropriate position on the adsorption surface of the negative pressure adsorption head, and the functional film can be arranged at an appropriate position on the bottom surface of the cell culture vessel. It becomes possible to arrange.

The schematic diagram explaining an example of the functional film negative-pressure-adsorbed to a negative-pressure adsorption head by the transfer apparatus by this invention. The schematic diagram explaining the original fabric which temporarily bonded many functional films to the strip | belt-shaped peeling film. The schematic diagram explaining an example of the manufacturing apparatus of the cell culture container provided with the transfer apparatus by this invention. It is a figure explaining an example of the negative pressure suction head used for the manufacturing apparatus shown in FIG. 3, Comprising: (a) is the longitudinal cross-sectional view, (b) is the bottom view. It is a figure explaining the transfer state of a functional film at the time of using the transfer apparatus by this invention, Comprising: (a) is the A section enlarged view of FIG. 3, (b) is a of FIG. Sectional drawing which follows the -a line. The figure explaining the state after the fall of a functional film at the time of using the transfer apparatus by this invention. The figure which shows the flask type cell culture container which is an example of the cell culture container which has arrange | positioned the functional film. The figure which shows the manufacturing procedure of the flask type cell culture container shown in FIG. The figure which shows the other manufacturing procedure of the flask type cell culture container shown in FIG. The figure explaining the fall state of a functional film at the time of using the transfer apparatus by this invention. The figure explaining the fall state of the culture sheet at the time of using the conventional transfer apparatus.

  Hereinafter, the present invention will be described based on embodiments. FIG. 1 is a cross-sectional view schematically illustrating an example of a functional film transferred by a negative pressure adsorption head according to the present invention. In the present invention, the functional film as a target is particularly a film having a pressure-sensitive adhesive layer having flexibility and having a desired function suitable for cell culture on the surface. It is not limited. In a preferred embodiment, like the functional film 10 shown in FIG. 1, the functional compound layer 12 is laminated on one surface of the film base layer 11, and the pressure-sensitive adhesive layer 13 is laminated on the other surface. In addition, in FIG. 1, 14 is a peeling film which makes the strip | belt shape mentioned later.

<Film base material layer 11>
Although not limited, the film base material layer 11 should just contain the material which can form the said functional compound layer 12 in one surface, and the kind of material is not specifically limited. Typically, as a material for the film base layer 11, polyethylene terephthalate (PET), polystyrene (PS), polycarbonate (PC), triacetyl cellulose (TAC), polyimide (PI), nylon (Ny), low density polyethylene (LDPE), medium density polyethylene (MDPE), vinyl chloride, vinylidene chloride, polyphenylene sulfide, polyether sulfone, polyethylene naphthalate, polypropylene, acrylic and the like.

  The surface of the film base material layer 11 on the side where the functional compound layer 12 is formed can be a surface subjected to an easy adhesion treatment. “Easy adhesion treatment” refers to treatment with an easy adhesive such as polyester, acrylic ester, polyurethane, polyethyleneimine, silane coupling agent, perfluorooctane sulfonic acid (PFOS), and the like.

  The thickness of the film base material layer 11 (when the film base material layer 11 includes an easy adhesion layer in addition to the base material layer, it refers to the entire thickness of the film base material layer including the easy adhesion layer) is particularly Although there is no restriction | limiting, It is preferable that it is the thickness which provides flexibility, for example, is 5 micrometers-400 micrometers, Preferably it is 50 micrometers-250 micrometers.

<Functional compound layer 12>
The functional compound constituting the functional compound layer 12 includes an organic compound or an inorganic compound, and more preferably has a surface that can be changed from cell adhesiveness to cell nonadhesiveness by a predetermined stimulus. Examples thereof include hydrophilic compounds such as a stimulus-responsive polymer and an ethylene glycol chain composed of one or more ethylene glycol units (CH ₂ —CH ₂ —O).

  The film thickness of the functional compound layer is, for example, preferably in the range of 0.5 nm to 300 nm, and more preferably in the range of 1 nm to 100 nm.

  Hereinafter, preferred embodiments of the “stimulus responsive polymer layer” and the “hydrophilic compound layer” will be described.

<Stimulus responsive polymer layer>
The functional organic compound layer is particularly preferably a stimulus-responsive polymer layer. The stimulus-responsive polymer layer is a layer containing a polymer in which the degree of surface cell adhesion is changed by a predetermined stimulus. Examples of the stimulus responsive polymer include a temperature responsive polymer, a pH responsive polymer, an ion responsive polymer, and a photoresponsive polymer. Among these, a temperature-responsive polymer is preferable because it is easy to give a stimulus.

  As the temperature-responsive polymer, for example, a polymer that exhibits cell adhesion at a temperature at which cells are cultured and exhibits cell non-adhesion at a temperature at which the produced cell sheet is peeled may be used. For example, a temperature-responsive polymer has improved affinity to surrounding water at temperatures below the critical dissolution temperature, and the polymer takes up water and swells to make it difficult for cells to adhere to the surface (cell non-adhesiveness). It is preferable to use a material exhibiting a property (cell adhesiveness) that makes the polymer shrink and easily adheres cells to the surface when water is desorbed from the polymer at a temperature equal to or higher than the same temperature. Such a critical solution temperature is called a lower critical solution temperature T. A temperature-responsive polymer having a lower critical solution temperature T of 0 ° C. to 80 ° C., more preferably 0 ° C. to 50 ° C. may be used. This is because the cells can be stably cultured when the lower critical solution temperature T is 0 ° C. to 80 ° C.

  Suitable temperature-responsive polymers include acrylic polymers or methacrylic polymers. Specific examples of suitable temperature-responsive polymers include poly-N-isopropylacrylamide (T = 32 ° C.), poly-Nn-propyl acrylamide (T = 21 ° C.), and poly-Nn-propyl methacrylamide. (T = 32 ° C.), poly-N-ethoxyethyl acrylamide (T = about 35 ° C.), poly-N-tetrahydrofurfuryl acrylamide (T = about 28 ° C.), poly-N-tetrahydrofurfuryl methacrylamide (T = About 35 ° C.), and poly-N, N-diethylacrylamide (T = 32 ° C.).

  As the pH responsive polymer and the ion responsive polymer, those suitable for the cell sheet to be prepared can be appropriately selected.

  The stimulus-responsive polymer layer is prepared by preparing a coating composition containing a monomer that forms a desired stimulus-responsive polymer by polymerization, and an organic solvent that can dissolve the monomer, and then applying the coating composition according to a conventional coating method. A coating film is formed by coating on the surface of the substrate, and then a polymer is formed by polymerizing monomers in the coating film by an appropriate means such as radiation irradiation. It can be formed by causing a grafting reaction with the polymer.

<Hydrophilic compound layer>
As another embodiment of the functional organic compound layer, hydrophilicity such as an ethylene glycol chain composed of one or more ethylene glycol units (an ethylene glycol chain composed of a plurality of ethylene glycol units can be referred to as a “polyethylene glycol chain”). A layer of a functional compound.

  The terminal of the ethylene glycol chain may be in a form blocked with a hydroxyl group, or the terminal of the ethylene glycol chain may be in a form in which another substance such as a biological substance is linked by a covalent bond.

  A layer containing an ethylene glycol chain whose end is blocked with a hydroxyl group can provide a hydrophilic surface to which cells are difficult to adhere.

  Bio-related substances that can be covalently bonded to the end of ethylene glycol chain include antigens, antibodies, DNA, RNA, peptides, hormones, enzymes, cytokines, sugar chains, lipids, coenzymes, enzyme inhibitors, cells, and other functions. The protein which has is included. Furthermore, the low molecular weight compound which has affinity with such a biological substance, and a high molecular compound are also contained in the range of a biological substance.

  In order to immobilize a hydrophilic compound layer such as an ethylene glycol chain on the surface of a resin film base layer, it can be physically adsorbed on the surface of the film base layer in advance. A primer layer containing a polysiloxane containing a functional group in a side chain that can form a covalent bond by reacting with a hydroxyl group at the end of the chain is provided. The functional group on the side chain of the polysiloxane is preferably a glycidyl group or an epoxy group. The primer layer can be formed by applying a silanol compound having a desired side chain to the surface of the film substrate layer, and performing condensation polymerization on the surface to convert it into polysiloxane.

  Next, the functional group of the primer layer and the hydroxyl group of polyethylene glycol in which two or more ethylene glycol or ethylene glycol units are repeated are reacted to form a covalent bond, thereby immobilizing the ethylene glycol chain. At this time, ethylene glycol or polyethylene glycol containing a catalytic amount of concentrated sulfuric acid is brought into contact with the primer layer.

  A layer containing ethylene glycol chains whose ends are blocked with hydroxyl groups is formed in this way.

  Furthermore, if necessary, at least one functional group capable of forming a covalent bond with another substance is directly or indirectly linked to one end of the ethylene glycol chain. The method for introducing the functional group is not particularly limited.

<Adhesive layer 13>
Examples of the pressure-sensitive adhesive constituting the pressure-sensitive adhesive layer 13 include polyester resin, acrylic ester resin, polyurethane resin, polyethyleneimine resin, silane coupling agent, and perfluorooctane sulfonic acid (PFOS). Among them, acrylic ester Resins, polyurethane resins and the like can be preferably used.

  Although the thickness of an adhesive layer is not specifically limited, It is preferable that they are 10 micrometers-300 micrometers, and it is more preferable that they are 20 micrometers-200 micrometers.

  The functional film 10 having the layer structure shown in FIG. 1 can be produced by an arbitrary method. In the present embodiment, the functional film 10 is produced as follows. That is, the pressure-sensitive adhesive layer 13 is applied to the entire surface of the strip-shaped release film 14, and the strip-shaped film base material layer 11 and the functional compound layer 12 having the same width are laminated thereon. As a result, as shown in the drawing, a long object 20 (see FIG. 2) is formed, which is composed of a four-layer structure of the release film 14, the pressure-sensitive adhesive layer 13, the film base material layer 11, and the functional compound layer 12. . The release film 14 is not particularly limited as long as it has the required strength and flexibility. For example, a release treatment such as a silicone release agent is applied to a film made of a resin such as polyethylene terephthalate or a foamed film thereof. Can be mentioned.

  A form (not shown) having an outer shape of the functional film 10 to be obtained is pushed down from the surface opposite to the release film 14 of the long object 20 so as to reach the surface of the release film 14. Thereby, in the functional compound layer 12, the film base layer 11, and the pressure-sensitive adhesive layer 13, a cut line 21 that forms the outer shape of the functional film 10 is formed. By performing the stamping operation on the long object 20 continuously at a predetermined interval, a long object 20 having a plurality of cut lines 21 as shown in FIG. 2 is obtained.

  There is no restriction | limiting in the shape in planar view of the functional film 10, It can take arbitrary shapes, and the formwork according to it can be used. In the example shown in FIG. 2, the functional film 10 intended to be placed on the bottom surface 51 </ b> A of the cell culture vessel 50 </ b> A having a tapered shape as shown in FIG. 7 is obtained. Is substantially the same shape as the shape of the inner peripheral contour of the bottom surface 51A of the cell culture vessel 50A.

  The long object 20 is wound into a roll so that the functional film 10 side is on the inside, and is made into a raw fabric 22 (see FIG. 3) and stored.

  FIG. 3 shows an example of an apparatus for separating the functional film 10 from the original fabric 22 and transferring the separated functional film 10 to the bottom surface 51 of the cell culture vessel 50. The transfer device 30 shown in the figure includes an air cylinder 31 attached to a conventionally known three-dimensional robot arm (not shown), and a negative pressure adsorption as shown in FIG. A head 40 is detachably mounted. The air cylinder 31 is movable in the XY axis direction, which is the surface direction of the suction surface 41, and the Z axis direction, which is the vertical direction, by a three-dimensional robot arm control device (not shown).

  As shown in FIG. 3, a guide base 32 extending in the horizontal direction or in a slightly inclined downward direction is located in the vicinity of the air cylinder 31, and the long object 20 unwound from the original fabric 22. Is guided by the guide roll 33, moves along the upper surface of the guide table 32, makes a U-turn at the tip of the guide table 32, and is then wound up by the take-up roll 34. When the long object 20 makes a U-turn at the tip of the guide base 32, the inner region defined by the cut line 21 is peeled off from the release film 14 that supports the adhesive material layer 13 side of the functional film 10, and guided. It is sent out in the direction of the extended line on the upper surface of the table 32. That is, the functional film 10 having a three-layer structure of the functional compound layer 12, the film base layer 11, and the pressure-sensitive adhesive layer 13 has the functional compound layer 12 on the upper surface side and the pressure-sensitive adhesive layer 13 side on the lower surface side. The film is peeled off and separated from the release film 14 continuously in a posture, and is sent out in a horizontal direction or a slightly inclined direction.

  The transfer device 30 further includes a transport conveyor 35 that can intermittently move and stop while the cell culture vessel 50 is placed in a horizontal posture.

  In addition, the transfer device 30 has an attitude in which an air nozzle 37 having a lateral width substantially equal to the lateral width of the functional film 10 is directed obliquely upward in the lower position of the functional film 10 delivered from the guide table 32. Multiple can be attached. Specifically, the first position near the tip of the guide base 32, the second position farther than the first position in the feeding direction of the functional film 10, and the feeding direction of the functional film 10 are orthogonal to each other. The air nozzles 37a to 37d are attached to the third and fourth positions on both sides of the functional film 10 fed in the direction of the movement. By receiving the air jets from these air nozzles 37 from below, the functional film 10 peeled off from the release film 14 and sent out can maintain a substantially horizontal posture until it is adsorbed to the suction surface 41 by negative pressure. .

  As shown in the longitudinal sectional view of FIG. 4A, the negative pressure suction head 40 has a suction surface 41 that is a substantially flat surface and an air chamber 42 on the back side of the suction surface 41. The air chamber 42 is connected to the air passage 36 of the air cylinder 31. The air passage 36 is connected to appropriate air suction and exhaust means (not shown). As shown in FIG. 4B, in this example, the shape of the suction surface 41 is circular in plan view, and a number of negative pressure suction holes 44 are formed in the suction surface 41. That is, unlike the material described in FIG. 2, circular cut lines 21 having substantially the same size as the suction surface 41 are formed on the original fabric 22 used in the transfer device 30 at regular intervals. As described above, the circular functional film 10 is continuously peeled from the release film 14 in a posture in which the pressure-sensitive adhesive layer 13 side is the lower surface side, and is sent out in a horizontal direction or a slightly inclined direction.

  Hereinafter, a state in which the functional film 10 is peeled from the release film 14 and is attracted to the suction surface 41 of the negative pressure suction head 40 will be specifically described. As shown in FIG. 5A, the robot arm control device (not shown) places the air cylinder 31 at a position where the fed-out functional film 10 can be negatively adsorbed by the negative pressure adsorption head 40. Position. And in order to maintain the attitude | position of the functional film 10 until it adsorb | sucks to the adsorption | suction surface 41 at a negative pressure, while injecting air from the air nozzle 37 to the functional film 10, the air in the air chamber 42 by a suction means , And a negative pressure is generated on the suction surface 41, whereby the functional film 10 is sucked by the negative pressure on the suction surface 41. Here, since the functional film 10 peeled from the release film 14 is sent out in the feeding direction (left direction in the figure), the position of the fed-out functional film 10 is detected by an appropriate detection means (not shown). Then, the position information detected by the detecting means is transmitted to the control unit 39 (see FIG. 3), and an ON signal is sequentially transmitted from the control unit 39 to the air nozzles 37a to 37d via the control line 39a, thereby guiding the guide table 32. Air is sequentially ejected from the air nozzles 37a to 37d in the order of close to. Note that the ON signal may be transmitted simultaneously from the control unit 39 to the air nozzles 37a to 37d, and air may be ejected from all of the air nozzles 37a to 37d at a time.

  Further, as shown in FIG. 5B, the air nozzle 37a and the air nozzle 37b are planes orthogonal to the feeding direction of the functional film 10 and on a plane P1 passing through the center of the suction surface 41 of the negative pressure suction head 40. Located on the opposite side. Further, the air nozzle 37c and the air nozzle 37d are arranged on the opposite side of the plane P2 passing through the center of the suction surface 41 of the negative pressure suction head 40, which is a plane orthogonal to the plane P1. Thus, by arranging the air nozzle 37a and the air nozzle 37b in the feeding direction of the functional film 10, for example, the functional film 10 having different physical property values is negatively adsorbed on the adsorption surface 41 of the negative pressure adsorption head 40. In this case, the position in the feeding direction of the functional film 10 until the negative pressure adsorption head 41 is negatively adsorbed by adjusting the air amount and air pressure of the air nozzle 37a and air nozzle 37b in advance is adjusted appropriately. can do. Further, since the air nozzle 37c and the air nozzle 37d are arranged in a direction orthogonal to the feeding direction of the functional film 10, for example, the functional film 10 having different physical property values is negatively applied to the suction surface 41 of the negative pressure suction head 40. When adsorbing, the air amount and air pressure of the air nozzle 37c and the air nozzle 37d are set appropriately in advance so that they are orthogonal to the feeding direction of the functional film 10 until the negative pressure adsorption head 40 adsorbs negative pressure on the adsorption surface 41. The position in the direction to be adjusted can be adjusted.

  Further, since the air nozzles 37a to 37d are provided with adjusting means 38a to 38d for adjusting the air amount and air pressure of the air ejected from the tip of the air nozzle, the functional film 10 is predetermined from the position information detected by the detecting means. When it is detected that the feed is shifted at a position deviated from the position, the control unit 39 transmits a control signal to the adjusting means 38a to 38d of the air nozzles 37a to 37d, and the adjusting means 38a to 38d. 38d is controlled to adjust the air amount and air pressure of the air ejected from each of the air nozzles 37a to 37d, thereby correcting the positional deviation of the functional film 10 in the feeding direction of the functional film 10 and the direction orthogonal to the feeding direction. Then, the functional film 10 is guided to an appropriate position on the suction surface 41 of the negative pressure suction head 40. For example, when the functional film 10 peeled off from the raw fabric 22 is sent out at a position shifted in the feed direction (left direction in the figure), the air amount or air pressure of the air jetted from the air nozzle 37b is increased, and the air nozzle The amount of air blown from 37a and the air pressure are reduced, the functional film 10 is guided in the direction opposite to the feeding direction (right direction in the figure), and the functional film 10 is moved to an appropriate position on the suction surface 41. Deploy.

  In addition, as a detection means which detects the position with respect to the adsorption | suction surface 41 of the functional film 10 sent out, an infrared sensor, an image processing apparatus, etc. can be mentioned, for example. For example, the position of the functional film 10 sent out by placing an infrared sensor in the vicinity of the tip of the guide table 32 is detected, the detection signal is transmitted to the control unit 39, and the air air blown from the air nozzle 37 The amount and air pressure can be controlled. Alternatively, the position of the functional film 10 sent out may be detected by an image processing device, and the detected image may be transmitted to the control unit 39 to control the air amount or air pressure of the air ejected from the air nozzle 37.

  Further, the adjusting means 38 uses, for example, a motor, and sends a drive signal to the motor from the control unit 39 based on the position information detected by the detecting means, and the motor is electrically driven to eject from the air nozzle 37. The air amount or air pressure of the air to be discharged may be controlled, or the user or the like manually adjusts the adjusting means such as a valve based on the position information detected by the detecting means, and the air jetted from the air nozzle 37 is controlled. The amount of air and air pressure may be controlled. Further, the air nozzle 37 may be provided with a position adjusting unit and an angle adjusting unit, and the position of the air nozzle 37 and the air ejection angle with respect to the functional film 10 may be adjusted based on the position information detected by the detecting unit.

  In addition, as gas ejected with respect to the lower surface of the functional film 10, other than air, for example, inert gas, such as nitrogen, can also be applied.

  Then, as described above, the position and orientation of the functional film 10 are controlled by the air nozzle 37, and the control device operates the robot arm in a state in which the functional film 10 is sucked to the suction surface 41 by a negative pressure. 31 is moved to a position immediately above the cell culture vessel 50 conveyed by the conveyor 35. That is, the cell culture container 50 is disposed below the functional film 10. After the movement, the air cylinder 31 is lowered until the distance between the bottom surface of the cell culture vessel 50 and the functional film 10 is about 0.1 mm to 10 mm, and the negative pressure is eliminated at the lowered position, and at the same time, the exhaust means is operated. Then, air of a predetermined pressure is sent into the air chamber 42. This air is discharged (exhaust) from each negative pressure adsorption hole 44 formed in the adsorption surface 41, and the functional film 10 falls toward the bottom surface of the cell culture vessel 50 by the discharge pressure of air in addition to its own weight. Note that the functional film 10 may be dropped toward the bottom surface of the cell culture container 50 only by gravity without releasing the air from each negative pressure adsorption hole 44 and eliminating the negative pressure.

  At this time, in the present embodiment, the functional film 10 is disposed at an appropriate position on the adsorption surface 41, and thus, for example, the cell culture vessel 50 can be separated from the adsorption surface 41 without contacting the side wall 52 of the cell culture vessel 50. It will drop toward the proper position. FIG. 6 shows a state after dropping in which the back surface of the functional film 10, that is, the entire surface on the pressure-sensitive adhesive layer 13 side is in contact with the bottom surface 51 of the cell culture vessel 50. It is fixed to the bottom surface 51 of the cell culture vessel 50 through the adhesive layer 13.

  In addition, the hole diameter, base number, and shape of the negative pressure adsorption hole 44 shown in FIG. 4B can be selected as appropriate. Although not shown, for example, the hole diameter in the vicinity of the center of the suction surface 41 can be increased or the number of holes can be increased, and as another example, the negative pressure suction hole 44 is formed by a plurality of parallel slits. You can also

  As described above, the shape of the suction surface 41 of the negative pressure suction head 40 is not limited to the circular shape described above, but is determined depending on the shape of the bottom surface 51 of the cell culture vessel 50 that transfers the adsorbed functional film 10. As the cell culture container 50, in addition to a dish-shaped or bowl-shaped container having an open top as shown in FIG. 6, a flask-shaped container 50A as shown in FIG.

  As shown in FIG. 7A, the flask-type cell culture container 50A includes a container part 100 and a lid 110. The container unit 100 includes at least a bottom surface 51A, a side wall portion 102 erected on the periphery of the bottom surface 51A, and a top surface portion 103 that is joined to the upper end portion of the side wall portion 102 and is opposed to the bottom surface 51A. The bottom surface 51A has a shape in which one end side of a rectangular flat plate is narrowed, and a through hole 104 is formed in the side wall portion 102 corresponding to the narrowed tip. And the neck part 105 extended to the outer side of the container part 100 from the periphery of the through-hole 104 is provided, and the lid | cover 110 is mounted | worn detachably there. By combining the container part 100 and the lid 110, a flask-type cell culture container 50A is formed.

  FIG. 7B shows a cross section along the line bb in FIG. 7A, and FIG. 7C shows a cross section along the line cc. In the internal space surrounded by the bottom surface 51 </ b> A, the side wall portion 102, and the top surface portion 103 of the container portion 100, an inner chamber 130 for containing cells and a culture medium is formed. The functional film 10 described above is fixed to a part of the bottom surface 51 </ b> A facing the inner chamber 130.

  The functional film 10 can be fixed when the surface to which the functional film 10 is fixed is not opened and is located in a closed container like the flask type cell culture container 50A. What is necessary is just to complete the target cell culture container by fixing the functional film 10 to a shape-shaped member, and combining the member after film fixation with another member.

  For example, as shown in FIG. 8, a first member 201 having a bottom surface 51A and a side wall portion 102, the side opposite to the fixing surface of the functional film 10 on the bottom surface 51A being opened, and the top surface portion 103 on the opened surface. The container part 100 is formed by joining the 2nd member 202 corresponding to these. At this time, before bonding the second member 202, the functional film 10 is fixed to the inner surface of the bottom surface 51A of the first member 201 by the method described above. The first member 201 and the second member 202 are joined in a liquid-tight manner by an appropriate method so that the culture solution does not leak out according to the purpose of cell culture, if necessary.

  In the embodiment shown in FIG. 9, the first member 301 corresponding to the bottom surface 51 </ b> A, the second member 302 corresponding to the side wall portion 102 including the neck portion 105, and the third member 303 corresponding to the top surface portion 103 are joined. Thereby, the container part 100 is formed. In this aspect, the functional film 10 is fixed to the portion of the first member 301 corresponding to the inner surface of the bottom surface 51A by the method described above.

  When the functional film 10 is transferred to the flask-type cell culture vessel 50A having the shape of the bottom surface 51A shown in FIGS. 7 to 9, one end side is narrow as shown in FIG. While using the long object 20 with the cut line 21 having the formed shape, the shape of the suction surface 41 of the negative pressure suction head 40 is also a shape corresponding thereto.

  In the case of such a shape, in the case of the cell culture container 50A shown in FIGS. 7 to 9 in one end side of the bottom surface 50A, in the region 54 on the end portion side opposite to the neck portion 105 of the bottom surface 51A, It may be desirable to allow the one end side of the falling functional film 10 to contact the fastest. Although not shown, in the case of the negative pressure suction head 40 having the shape of the suction surface 41 along the shape of the cut line 21 shown in FIG. 2, the amount of air exhausted is large in the region 15 indicated by the oblique lines in FIG. By setting the hole diameter and radix of the negative pressure adsorption hole 44 so that the region is formed, the functional film 10 can contact the bottom surface 51A most quickly in the region 54 on the side opposite to the neck 105. It becomes.

  Accordingly, as shown in FIG. 10 (a), the functional film 10 comes into contact with the bottom surface of the cell culture container 50A sequentially from one end side of the bottom surface 51A of the cell culture container 50A. It is possible to reliably avoid bubbles from entering between the bottom surface 51A. Further, as shown in FIG. 10B, even when the bottom surface 51A of the cell culture vessel 50A has the horizontal portion 55 and the upward inclined portion 56, the opposite side of the inclined portion 56 of the horizontal portion 55. By setting the hole diameter and the radix of the negative pressure adsorption hole 44 so that the amount of air exhausted is increased at a position where the one end side of the falling functional film 10 can be brought into contact with the end region of the film most quickly. It becomes possible to arrange the conductive film 10 at an appropriate position on the bottom surface 51A of the cell culture vessel 50A in the absence of bubbles.

  In the present invention, the material constituting each cell culture vessel is not particularly limited, and materials generally used in cell culture can be used. For example, polystyrene resin, polyester resin, polyethylene resin, polyethylene terephthalate resin, polypropylene resin, ABS resin, nylon, acrylic resin, fluorine resin, polycarbonate resin, polyurethane resin, methylpentene resin, phenol resin, melamine resin, epoxy resin, vinyl chloride A resin material such as a resin, a resin material containing at least one of the above-described surface hydrophilized treatment, and an inorganic material such as glass or quartz are preferable, but a resin material is preferable. The resin material is preferably a polystyrene resin or a polyethylene terephthalate resin.

10 ... functional film,
11 ... film base material layer,
12 ... Functional compound layer,
13 ... adhesive layer,
14 ... stripping release film,
20 ... long object composed of four layers,
21 ... a score line forming the outer shape of the functional film,
22 ... roll-shaped original fabric,
30 ... Transfer device,
31 ... Air cylinder,
32 ... Information desk,
33 ... Guide roll,
34 ... take-up roll,
35 ... Conveyor for cell culture container,
36 ... Air path of air cylinder,
37 (37a-37d) ... air nozzle,
38 (38a-38d) ... adjusting means,
39 ... control unit,
39a ... control line,
40 ... negative pressure suction head,
41 ... adsorption surface,
42 ... Air chamber,
44 ... negative pressure adsorption hole,
50, 50A ... cell culture vessel,
51, 51A ... bottom of cell culture vessel,
52. Side wall portion of cell culture container

Claims (6)

A transfer device for peeling the functional film supported by the release film from the release film and adsorbing the negative pressure on the adsorption surface having the negative pressure adsorption holes,
The transfer device includes an air nozzle that ejects gas toward the lower surface of the functional film peeled from the release film, and the functional film that has received the gas ejected from the air nozzle is loaded on the suction surface. A transfer device characterized in that the functional film is dropped onto a cell culture container disposed under the functional film while being adsorbed with pressure and eliminating the negative pressure .   The transfer device according to claim 1, wherein the transfer device includes a plurality of the air nozzles.   The said transfer apparatus is provided with at least 2 air nozzle in the feed direction of this functional film at the time of making the said functional film adsorb | suck to the said adsorption surface by negative pressure, The transfer apparatus of Claim 2 characterized by the above-mentioned.   The transfer device includes at least two air nozzles in a direction orthogonal to a feeding direction of the functional film when the functional film is adsorbed to the adsorption surface by negative pressure. The transfer device described.   5. The transfer device according to claim 1, further comprising an adjusting unit that adjusts a flow rate and / or pressure of a gas ejected from the air nozzle. 6. A raw fabric comprising a functional film having a functional organic compound layer laminated on one side and a pressure-sensitive adhesive layer laminated on the other side, and a release film supporting the pressure-sensitive adhesive layer side of the functional film. A process to prepare;
Transferring the original fabric in a posture with the functional organic compound layer of the functional film as the upper surface side;
Separating the functional film from the original fabric by peeling the functional film from the release film;
Ejecting gas to the lower surface of the functional film separated from the original fabric, and negatively adsorbing the functional film that has undergone gas ejection on an adsorption surface having negative pressure adsorption holes;
A cell culture container is disposed below the functional film, the negative pressure is eliminated, the functional film is dropped onto the cell culture container, and the functional film is placed on the cell culture container via the adhesive layer. And a step of arranging the cell culture container.

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