JP5852584B2 - 球状端の入射および出力光ファイバを使用したバイオアナリシス - Google Patents
球状端の入射および出力光ファイバを使用したバイオアナリシス Download PDFInfo
- Publication number
- JP5852584B2 JP5852584B2 JP2012551358A JP2012551358A JP5852584B2 JP 5852584 B2 JP5852584 B2 JP 5852584B2 JP 2012551358 A JP2012551358 A JP 2012551358A JP 2012551358 A JP2012551358 A JP 2012551358A JP 5852584 B2 JP5852584 B2 JP 5852584B2
- Authority
- JP
- Japan
- Prior art keywords
- separation
- detection
- optical waveguide
- radiation
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000013307 optical fiber Substances 0.000 title claims description 16
- 238000011953 bioanalysis Methods 0.000 title description 6
- 238000001514 detection method Methods 0.000 claims description 126
- 238000000926 separation method Methods 0.000 claims description 90
- 230000003287 optical effect Effects 0.000 claims description 44
- 230000005855 radiation Effects 0.000 claims description 40
- 238000005251 capillar electrophoresis Methods 0.000 claims description 34
- 238000000034 method Methods 0.000 claims description 13
- 238000001962 electrophoresis Methods 0.000 claims description 9
- 230000001154 acute effect Effects 0.000 claims 3
- 230000001939 inductive effect Effects 0.000 claims 1
- 239000000835 fiber Substances 0.000 description 89
- 230000005284 excitation Effects 0.000 description 42
- 239000000523 sample Substances 0.000 description 19
- 238000004458 analytical method Methods 0.000 description 13
- 239000011162 core material Substances 0.000 description 13
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 12
- 239000000872 buffer Substances 0.000 description 11
- 238000013461 design Methods 0.000 description 11
- 230000035945 sensitivity Effects 0.000 description 9
- 230000008878 coupling Effects 0.000 description 8
- 238000010168 coupling process Methods 0.000 description 8
- 238000005859 coupling reaction Methods 0.000 description 8
- 238000003018 immunoassay Methods 0.000 description 8
- 239000000499 gel Substances 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 108020004414 DNA Proteins 0.000 description 6
- 238000001917 fluorescence detection Methods 0.000 description 6
- 238000002347 injection Methods 0.000 description 6
- 239000007924 injection Substances 0.000 description 6
- 238000001499 laser induced fluorescence spectroscopy Methods 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 239000012491 analyte Substances 0.000 description 4
- 238000013459 approach Methods 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 150000001720 carbohydrates Chemical class 0.000 description 4
- 235000014633 carbohydrates Nutrition 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 239000004005 microsphere Substances 0.000 description 4
- 238000003860 storage Methods 0.000 description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 3
- 239000000975 dye Substances 0.000 description 3
- 238000001502 gel electrophoresis Methods 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 238000004088 simulation Methods 0.000 description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 2
- 102000029797 Prion Human genes 0.000 description 2
- 108091000054 Prion Proteins 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 229940098773 bovine serum albumin Drugs 0.000 description 2
- 238000005253 cladding Methods 0.000 description 2
- 238000005094 computer simulation Methods 0.000 description 2
- 238000005370 electroosmosis Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000007850 fluorescent dye Substances 0.000 description 2
- 230000004927 fusion Effects 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000011896 sensitive detection Methods 0.000 description 2
- 238000007873 sieving Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000007790 solid phase Substances 0.000 description 2
- 238000001712 DNA sequencing Methods 0.000 description 1
- 102000008394 Immunoglobulin Fragments Human genes 0.000 description 1
- 108010021625 Immunoglobulin Fragments Proteins 0.000 description 1
- VAYOSLLFUXYJDT-RDTXWAMCSA-N Lysergic acid diethylamide Chemical compound C1=CC(C=2[C@H](N(C)C[C@@H](C=2)C(=O)N(CC)CC)C2)=C3C2=CNC3=C1 VAYOSLLFUXYJDT-RDTXWAMCSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 229920001410 Microfiber Polymers 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 239000004642 Polyimide Substances 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000013480 data collection Methods 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 238000000295 emission spectrum Methods 0.000 description 1
- 238000005530 etching Methods 0.000 description 1
- 239000013305 flexible fiber Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000005350 fused silica glass Substances 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 239000003658 microfiber Substances 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000036963 noncompetitive effect Effects 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 229920001721 polyimide Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 208000008864 scrapie Diseases 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 238000004557 single molecule detection Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000012549 training Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/74—Optical detectors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- Immunology (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biophysics (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Optical Measuring Cells (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US29930110P | 2010-01-28 | 2010-01-28 | |
| US61/299,301 | 2010-01-28 | ||
| PCT/US2011/023071 WO2011094648A2 (en) | 2010-01-28 | 2011-01-28 | Bio-analysis using ball-ended incident and output optical fiber |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013518290A JP2013518290A (ja) | 2013-05-20 |
| JP2013518290A5 JP2013518290A5 (enExample) | 2014-03-20 |
| JP5852584B2 true JP5852584B2 (ja) | 2016-02-03 |
Family
ID=43983625
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012551358A Active JP5852584B2 (ja) | 2010-01-28 | 2011-01-28 | 球状端の入射および出力光ファイバを使用したバイオアナリシス |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US8784626B2 (enExample) |
| EP (1) | EP2529211B8 (enExample) |
| JP (1) | JP5852584B2 (enExample) |
| KR (1) | KR101712691B1 (enExample) |
| CN (1) | CN102667463B (enExample) |
| WO (1) | WO2011094648A2 (enExample) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2609420B1 (en) * | 2010-08-24 | 2021-03-03 | Bioptic, Inc. | Disposible bio-analysis cartridge and instrument for conducting bio-analysis using same |
| EP2717424B1 (en) * | 2011-05-31 | 2018-02-21 | LG Chem, Ltd. | Apparatus for leveling voltage for connecting unit racks for storing power, and system for storing power comprising same |
| US20150338347A1 (en) * | 2014-05-22 | 2015-11-26 | Bioptic, Inc. | Glycan profiling utilizing capillary electrophoresis |
| CN109475795B (zh) * | 2016-05-13 | 2021-06-01 | 安捷伦科技有限公司 | 用于自动对准、校准和标准化电泳数据的系统和方法 |
| USD859685S1 (en) | 2017-02-21 | 2019-09-10 | Bioptic, Inc. | Disposable bio-analysis cartridge |
| KR20240159580A (ko) | 2022-03-10 | 2024-11-05 | 바이옵틱, 아이엔시. | 열순환기가 달린 휴대용 전기영동 시스템 |
| DE112024000232T5 (de) * | 2023-03-24 | 2025-09-04 | Hitachi High-Tech Corporation | Kapillarelektrophoresevorrichtung |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4675300A (en) * | 1985-09-18 | 1987-06-23 | The Board Of Trustees Of The Leland Stanford Junior University | Laser-excitation fluorescence detection electrokinetic separation |
| US4985129A (en) * | 1988-12-02 | 1991-01-15 | Bio-Rad Laboratories, Inc. | Apparatus for capillary electrophoresis |
| CA2094343A1 (en) * | 1992-07-17 | 1994-01-18 | Gerald L. Klein | Method and apparatus for displaying capillary electrophoresis data |
| JP3515646B2 (ja) * | 1995-09-18 | 2004-04-05 | 大塚電子株式会社 | マルチキャピラリ電気泳動装置 |
| US6592733B1 (en) | 1999-11-12 | 2003-07-15 | Motorola, Inc. | Capillary electrophoresis devices incorporating optical waveguides |
| US6184990B1 (en) | 1999-12-22 | 2001-02-06 | Beckman Coulter, Inc. | Miniature multiple wavelength excitation and emission optical system and method for laser-induced fluorescence detectors in capillary electrophoresis |
| JP3893849B2 (ja) * | 2000-05-15 | 2007-03-14 | 株式会社日立製作所 | キャピラリアレイ電気泳動装置及び電気泳動方法 |
| JP3969699B2 (ja) | 2000-11-17 | 2007-09-05 | 日本板硝子株式会社 | マイクロ化学システム用チップ部材、及び該チップ部材を用いたマイクロ化学システム |
| WO2002044703A2 (en) * | 2000-12-01 | 2002-06-06 | Cetek Corporation | High throughput capillary electrophoresis system |
| EP1354192B1 (en) * | 2001-01-26 | 2011-08-31 | Qiagen Sciences, LLC | Multi-channel bio-separation cartridge |
| CA2436098C (en) | 2001-01-26 | 2014-04-01 | Biocal Technology, Inc. | Optical detection in a multi-channel bio-separation system |
| US6870165B2 (en) * | 2001-10-19 | 2005-03-22 | Biocal Technology, Inc. | Multi-color multiplexed analysis in a bio-separation system |
| GB2383127B (en) * | 2001-12-12 | 2004-10-20 | Proimmune Ltd | Device and method for investigating analytes in liquid suspension or solution |
| US7250099B2 (en) * | 2002-12-13 | 2007-07-31 | Biocal Technology, Inc. | Optical detection alignment coupling apparatus |
| US20040047535A1 (en) * | 2002-09-09 | 2004-03-11 | Ljerka Ukrainczyk | Enhanced fiber-optic sensor |
| JP2004361239A (ja) | 2003-06-04 | 2004-12-24 | Enplas Corp | マイクロ分析システム用光学系 |
| US7924425B2 (en) * | 2005-06-27 | 2011-04-12 | The United States Of America As Represented By The Department Of Health And Human Services | Spatially selective fixed-optics multicolor fluorescence detection system for a multichannel microfluidic device, and method for detection |
| US20070131870A1 (en) * | 2005-12-12 | 2007-06-14 | Combisep | Multiplexed CE fluorescence system |
| JP2008116395A (ja) * | 2006-11-07 | 2008-05-22 | Fujitsu Ltd | 蛍光検出装置 |
-
2011
- 2011-01-28 KR KR1020127022024A patent/KR101712691B1/ko active Active
- 2011-01-28 US US13/016,944 patent/US8784626B2/en active Active
- 2011-01-28 WO PCT/US2011/023071 patent/WO2011094648A2/en not_active Ceased
- 2011-01-28 CN CN201180001774.0A patent/CN102667463B/zh active Active
- 2011-01-28 JP JP2012551358A patent/JP5852584B2/ja active Active
- 2011-01-28 EP EP11711406.6A patent/EP2529211B8/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP2529211B8 (en) | 2017-10-04 |
| KR20120112821A (ko) | 2012-10-11 |
| US8784626B2 (en) | 2014-07-22 |
| CN102667463B (zh) | 2016-09-07 |
| KR101712691B1 (ko) | 2017-03-06 |
| EP2529211A2 (en) | 2012-12-05 |
| WO2011094648A3 (en) | 2011-11-03 |
| JP2013518290A (ja) | 2013-05-20 |
| EP2529211B1 (en) | 2017-05-03 |
| US20110253540A1 (en) | 2011-10-20 |
| WO2011094648A2 (en) | 2011-08-04 |
| CN102667463A (zh) | 2012-09-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP5852584B2 (ja) | 球状端の入射および出力光ファイバを使用したバイオアナリシス | |
| US8778155B2 (en) | Disposible bio-analysis cartridge and instrument for conducting bio-analysis using same | |
| CN101652658B (zh) | 使用透明涂层的毛细管的毛细电泳 | |
| US11531004B2 (en) | Disposable multi-channel bio-analysis cartridge and capillary electrophoresis system for conducting bio-analysis using same | |
| US12461065B2 (en) | Glycan profiling utilizing capillary electrophoresis | |
| US6529275B2 (en) | Optical detection in bio-separation device using a widened detection zone | |
| JP6933730B2 (ja) | 使い捨てマルチチャネルバイオ分析カートリッジ、及びそれを用いてバイオ分析を実施するためのキャピラリー電気泳動システム | |
| US6929779B2 (en) | Optical detection in bio-separation device using axial radiation output | |
| JP3701283B2 (ja) | 電気泳動システムのためのエネルギービームガイド | |
| US6932940B2 (en) | Optical detection in bio-separation device using axial radiation input | |
| WO2003001200A2 (en) | Optical detection in bio-separation device having axial radiation input and output and widened detection zone | |
| AU2002254750A1 (en) | Energy beam guide for an electrophoresis system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20140128 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20140128 |
|
| A711 | Notification of change in applicant |
Free format text: JAPANESE INTERMEDIATE CODE: A711 Effective date: 20140207 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A821 Effective date: 20140207 |
|
| A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20140822 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20140909 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20141209 |
|
| A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20141218 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20150305 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20150609 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20151104 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20151204 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 5852584 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |