JP5791724B2 - 核酸を断片化する方法及び装置 - Google Patents

核酸を断片化する方法及び装置 Download PDF

Info

Publication number
JP5791724B2
JP5791724B2 JP2013530355A JP2013530355A JP5791724B2 JP 5791724 B2 JP5791724 B2 JP 5791724B2 JP 2013530355 A JP2013530355 A JP 2013530355A JP 2013530355 A JP2013530355 A JP 2013530355A JP 5791724 B2 JP5791724 B2 JP 5791724B2
Authority
JP
Japan
Prior art keywords
sample
shear
channel
shear region
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2013530355A
Other languages
English (en)
Japanese (ja)
Other versions
JP2013540439A5 (https=
JP2013540439A (ja
Inventor
デュリン，ギヨーム
ローハーン，ジェームズ，エー．，ジュニア
Original Assignee
コバリス，インコーポレイテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by コバリス，インコーポレイテッド filed Critical コバリス，インコーポレイテッド
Publication of JP2013540439A publication Critical patent/JP2013540439A/ja
Publication of JP2013540439A5 publication Critical patent/JP2013540439A5/ja
Application granted granted Critical
Publication of JP5791724B2 publication Critical patent/JP5791724B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • B01L3/50215—Test tubes specially adapted for centrifugation purposes using a float to separate phases
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0631—Purification arrangements, e.g. solid phase extraction [SPE]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0663—Stretching or orienting elongated molecules or particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/141—Preventing contamination, tampering
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/042—Caps; Plugs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0838—Capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0841—Drums
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0854—Double walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0463—Hydrodynamic forces, venturi nozzles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B01L3/50255—Multi-well filtration
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2013530355A 2010-09-24 2011-09-23 核酸を断片化する方法及び装置 Active JP5791724B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US38639210P 2010-09-24 2010-09-24
US61/386,392 2010-09-24
PCT/US2011/052959 WO2012040571A2 (en) 2010-09-24 2011-09-23 Method and apparatus for fragmenting nucleic acids

Publications (3)

Publication Number Publication Date
JP2013540439A JP2013540439A (ja) 2013-11-07
JP2013540439A5 JP2013540439A5 (https=) 2014-11-06
JP5791724B2 true JP5791724B2 (ja) 2015-10-07

Family

ID=45871060

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2013530355A Active JP5791724B2 (ja) 2010-09-24 2011-09-23 核酸を断片化する方法及び装置

Country Status (4)

Country Link
US (1) US9108194B2 (https=)
EP (1) EP2619302B1 (https=)
JP (1) JP5791724B2 (https=)
WO (1) WO2012040571A2 (https=)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102011054474B4 (de) * 2011-07-20 2014-02-13 Stratec Biomedical Ag System zur Stabilisierung, Aufbewahrung und Lagerung einer Nukleinsäure
US20140370499A1 (en) * 2011-12-23 2014-12-18 The Broad Institute, Inc. Device and method for fragmenting polymers and particles
AU2013202805B2 (en) * 2013-03-14 2015-07-16 Gen-Probe Incorporated System and method for extending the capabilities of a diagnostic analyzer
JP6410464B2 (ja) * 2014-05-12 2018-10-24 三井電気精機株式会社 Ｄｎａ断片化装置及びｄｎａ断片化方法
WO2016145039A1 (en) 2015-03-10 2016-09-15 Rubicon Genomics, Inc. Enhanced fragmentation and repair of nucleic acids
KR102701140B1 (ko) * 2022-07-25 2024-09-02 주식회사 제론셀베인 균일한 크기의 핵산 단편 제조 방법

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0552290B1 (en) 1990-10-11 2000-02-02 Advanced Research & Technology Institute Process and apparatus for fragmenting biomaterials
JPH08295025A (ja) * 1995-04-26 1996-11-12 Hitachi Ltd インクジェット記録装置
US5846832A (en) * 1995-09-20 1998-12-08 The Board Of Trustees Of The Leland Stanford Junior University Apparatus and method for shear breakage of polynucleotides
US6622335B1 (en) * 2000-03-29 2003-09-23 Lam Research Corporation Drip manifold for uniform chemical delivery
CA2419961A1 (en) * 2000-08-18 2002-02-28 Integrigen, Inc. Methods and compositions for directed molecular evolution using dna-end modification
GB2394912B (en) 2002-11-01 2006-07-12 Norchip As A microfabricated fluidic device for fragmentation
AU2003278682B2 (en) * 2002-11-18 2007-07-19 Agency For Science, Technology And Research Method and system for cell and/or nucleic acid molecules isolation
US20060133957A1 (en) * 2003-01-17 2006-06-22 Knapp Merrill A Device and method for fragmenting material by hydrodynamic shear
JP4338139B2 (ja) * 2005-03-29 2009-10-07 ツネイシホールディングス株式会社 液滴噴射ノズル
EP2250286A1 (en) 2008-02-15 2010-11-17 Life Technologies Corporation Methods and compositions for shearing of polymers by sonication
US8252250B2 (en) * 2008-11-26 2012-08-28 Illumina, Inc. Electroosmotic pump with improved gas management

Also Published As

Publication number Publication date
WO2012040571A3 (en) 2012-05-31
EP2619302A4 (en) 2016-08-10
US20120077283A1 (en) 2012-03-29
US9108194B2 (en) 2015-08-18
EP2619302A2 (en) 2013-07-31
JP2013540439A (ja) 2013-11-07
EP2619302B1 (en) 2020-05-13
WO2012040571A2 (en) 2012-03-29

Similar Documents

Publication Publication Date Title
JP5791724B2 (ja) 核酸を断片化する方法及び装置
CN112236218B (zh) 用于连续流乳液处理的系统和方法
CN109908986B (zh) 一种基于出口不对称毛细管的液滴生成系统及使用方法
US8741661B2 (en) Methods and devices for sampling flowable materials
CN114534806B (zh) 用于封装和分割试剂的流体装置、系统和方法及其应用
JP4133803B2 (ja) 核酸精製構造体
CN110684650B (zh) 用于数字pcr检测的液滴生成系统及数字pcr检测方法
US20170037359A1 (en) System, apparatus and method for material preparation and/or handling
Li et al. A minimalist approach for generating picoliter to nanoliter droplets based on an asymmetrical beveled capillary and its application in digital PCR assay
CN216224463U (zh) 数字pcr液滴或单细胞液滴的生成装置及液滴生成管
CN113318796A (zh) 离心式微滴生成芯片
US20140193857A1 (en) Centrifuge tube droplet generator
US20210245153A1 (en) Analyte capturing devices with fluidic ejection devices
JP2014124097A (ja) 核酸分析用カートリッジ及び核酸分析装置
CN110684828B (zh) 数字pcr芯片、数字pcr检测系统及检测方法
US20060204403A1 (en) Micro-fluidic fluid separation device and method
JP5599266B2 (ja) 反応プレートの吸引デバイスおよび洗浄デバイス
CN109985681B (zh) 一种微液滴产生装置
CN110064453B (zh) 微液滴生成装置及生成方法
CN119351209A (zh) 一种核酸分析卡盒及液滴生成系统

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20140919

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20140919

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20150520

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20150521

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20150616

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20150708

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20150804

R150 Certificate of patent or registration of utility model

Ref document number: 5791724

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250