JP5735065B2 - 液体クロマトグラフィー用の移動相調整装置 - Google Patents
液体クロマトグラフィー用の移動相調整装置 Download PDFInfo
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/36—Control of physical parameters of the fluid carrier in high pressure liquid systems
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
- G01N2030/347—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient mixers
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Description
Claims (20)
- 高圧液体クロマトグラフィーシステムと共に使用するための移動相調整装置(200; 400)であって、
複数の入口(252; 452)を有し、各入口(252; 452)が溶媒源に流体連結され、該入口(252; 452)が移動相出口(254; 454)に流体連結され、該出口が複数の溶媒(A, B, C, D)を含む移動相を分析ラインに吐出する、マニフォルド(250; 450)と、
該マニフォルド(250; 450)に溶媒(A, B, C, D)を供給するための少なくとも1つのポンプ(202A, 202B; 402A, 402B)と、
前記各入口(252; 452)を通って供給された前記溶媒(A, B, C, D)が互いに混合して前記移動相を形成する位置に配置され、圧力センサー(208; 408)とパージバルブ(210; 410)のうち少なくとも1つに流体連結されている、マニフォルド分岐(256; 456)と、を含む、装置。 - 前記移動相出口(254; 454)で前記分析ラインに流体連結されており、前記移動相中の溶媒(A, B, C, D)の不均一な分配を均一化する、分配ホモジナイザー(212; 412)を更に含む、請求項1に記載の装置。
- 前記分配ホモジナイザー(212; 412)が、ミキサーカートリッジを含む、請求項2に記載の装置。
- 少なくとも1つの前記ポンプ(202A, 202B; 402A, 402B)が単一ピストンポンプである、請求項1〜請求項3のいずれか一項に記載の装置。
- 前記圧力センサー(208; 408)が、最高約20,000 psi (約1.4×108 パスカル) で作動するよう構成されている、請求項1〜請求項4のいずれか一項に記載の装置。
- 前記パージバルブ(210; 410)が、前記圧力センサー(208; 408)に流体連結されている、請求項1〜請求項5のいずれか一項に記載の装置。
- 前記圧力センサー(208; 408)が圧力トランスデューサーである、請求項1〜請求項6のいずれか一項に記載の装置。
- 前記圧力トランスデューサーが、液体の押しのけ容積を測定するダイヤフラムセンサーを含む、請求項7に記載の装置。
- 前記分岐(256; 456)が、前記移動相出口に連結されている第1出口(254, 454)と、第2出口(258; 458)とを含む、請求項1〜請求項8のいずれか一項に記載の装置。
- 前記マニフォルド(250)が、2つの溶媒(A, B)を受け取るための2つの入口(252)を有し、該2つの入口(252)並びに前記第1及び第2出口(254, 258)がそれぞれ交差接続(206)で連結されている、請求項9に記載の装置。
- 異なる溶媒(A, B, C, D)の送出力を経時的に変化させて前記移動相の組成を変えるよう、少なくとも1つの前記ポンプ(202A, 202B; 402A, 402B)を操作するコントローラーを更に含む、請求項1〜請求項10のいずれか一項に記載の装置。
- 少なくとも1つの前記ポンプ(202A, 202B; 402A, 402B)の出口と前記マニフォルド(250; 450)の入口(252; 452)とを直接に流体接続する配管を更に含み、ここにおいて該配管の長さは、ポンプ(202A, 202B; 402A, 402B)とマニフォルド(250; 450)との間の流体容積を低減するよう最小限に抑えられる、請求項1〜請求項11のいずれか一項に記載の装置。
- 前記圧力センサー(208; 408)から圧力測定データを取得して少なくとも1つの前記ポンプ(202A, 202B; 402A, 402B)の動作を該圧力測定データの関数として調節するような、コントローラーを更に含む、請求項1〜請求項12のいずれか一項に記載の装置。
- 前記マニフォルド(250; 450)が、流体ライン上の耐高圧性流体接続を最小限に抑えるため、機械加工された材料ブロック(240; 440)として構成される、請求項1〜請求項13のいずれか一項に記載の装置。
- 高圧液体クロマトグラフィーの稼働中の移動相調整装置の作動方法であって、次の工程:
(a) 溶媒源からマニフォルド入口へ溶媒を送出する工程、
(b) 該溶媒が該マニフォルド内の合流部分を通過し、ここで該溶媒が少なくとも他の1種類の溶媒と合わせられ、別のライン及び入口を通って該マニフォルドへと供給されて、移動相を形成する工程、
(c) 結果として得られた該移動相が該マニフォルドから分析ラインへと吐出される工程、及び
(d) 圧力測定と排気ベントのうち少なくとも1つを実施するために、該移動相流の一部分が、合流領域部分で分岐する工程、を含む、方法。 - 前記圧力測定の結果が、前記ポンプ送出の制御に使用される、請求項15に記載の方法。
- 2液、3液、又は4液の使用構成で、第1溶媒が、1つ、2つ、及び3つの他の溶媒とそれぞれ混合されて、前記移動相を形成する、請求項15又は請求項16に記載の方法。
- 高圧液体クロマトグラフィーシステムと共に使用するための溶媒混合装置であって、
複数の入口を有し、ここにおいて各入口が溶媒源と流体連結するよう構成され、更に、溶媒又は溶媒混合物からなる移動相を分析ラインに吐出するよう構成された移動相出口とを有するマニフォルドと、
圧力センサーとパージバルブのうち少なくとも1つに流体連結され、前記溶媒どうしが混合して前記移動相を形成する地点に配置された分岐に配管された行き止まり導管と、を含む、溶媒混合装置。 - 耐高圧性材料のブロックを更に含み、これに少なくとも前記マニフォルド、前記行き止まり導管、分析導管の一部、前記圧力センサーとパージバルブのうち少なくとも1つ、及び対応する流体ラインが組み込まれ、これによって該流体ラインの耐高圧性流体接続が不要になる、請求項18に記載の溶媒混合装置。
- 分配ホモジナイザーを更に含み、この流体ラインが前記ブロック内に組み込まれ、前記分析導管の一部に配管される、請求項19に記載の溶媒混合装置。
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US13/602,172 US8658034B1 (en) | 2012-09-02 | 2012-09-02 | Mobile phase preparation device for liquid chromatography |
US13/602,172 | 2012-09-02 |
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CN107715498A (zh) * | 2017-06-09 | 2018-02-23 | 利穗科技(苏州)有限公司 | 一种在线配液层析系统 |
US11307181B1 (en) | 2018-07-14 | 2022-04-19 | Sielc Technologies Corporation | HPLC system with mixed-mode columns for measuring charged analytes in complex mixtures |
CN110251993B (zh) * | 2019-07-19 | 2023-04-28 | 扬州罗素生物技术有限公司 | 一种基于大孔树脂吸附的中药多组分分离的集成装置 |
WO2022212504A1 (en) * | 2021-03-31 | 2022-10-06 | Waters Technologies Corporation | Solvent delivery with a reduced volume single stroke pumping arrangement providing continuous solvent flow |
CN116879474A (zh) * | 2023-07-11 | 2023-10-13 | 常州三泰科技有限公司 | 一种流动相比例控制装置及其控制方法 |
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US20140061128A1 (en) | 2014-03-06 |
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EP2703809B1 (en) | 2016-09-28 |
EP2703809A3 (en) | 2014-10-29 |
US8658034B1 (en) | 2014-02-25 |
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