JP5711554B2 - トリクロロエテンの完全脱塩素可能な微生物と当該微生物を用いるトリクロロエテンを含有する汚染物の浄化方法 - Google Patents
トリクロロエテンの完全脱塩素可能な微生物と当該微生物を用いるトリクロロエテンを含有する汚染物の浄化方法 Download PDFInfo
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- JP5711554B2 JP5711554B2 JP2011020655A JP2011020655A JP5711554B2 JP 5711554 B2 JP5711554 B2 JP 5711554B2 JP 2011020655 A JP2011020655 A JP 2011020655A JP 2011020655 A JP2011020655 A JP 2011020655A JP 5711554 B2 JP5711554 B2 JP 5711554B2
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- Prior art keywords
- dehalococcoides
- microorganism
- trichloroethene
- microorganisms
- genus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000000746 purification Methods 0.000 title claims description 26
- 239000000356 contaminant Substances 0.000 title claims description 7
- XSTXAVWGXDQKEL-UHFFFAOYSA-N Trichloroethylene Chemical compound ClC=C(Cl)Cl XSTXAVWGXDQKEL-UHFFFAOYSA-N 0.000 title description 31
- 229960002415 trichloroethylene Drugs 0.000 title description 31
- 230000000382 dechlorinating effect Effects 0.000 title description 6
- BZHJMEDXRYGGRV-UHFFFAOYSA-N Vinyl chloride Chemical class ClC=C BZHJMEDXRYGGRV-UHFFFAOYSA-N 0.000 claims description 48
- 241000880396 Dehalococcoides Species 0.000 claims description 47
- 241000894006 Bacteria Species 0.000 claims description 41
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- 238000004458 analytical method Methods 0.000 description 15
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- WSLDOOZREJYCGB-UHFFFAOYSA-N 1,2-Dichloroethane Chemical compound ClCCCl WSLDOOZREJYCGB-UHFFFAOYSA-N 0.000 description 3
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- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 3
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- UBOXGVDOUJQMTN-UHFFFAOYSA-N 1,1,2-trichloroethane Chemical compound ClCC(Cl)Cl UBOXGVDOUJQMTN-UHFFFAOYSA-N 0.000 description 2
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- 241001599461 Dehalococcoides mccartyi BAV1 Species 0.000 description 2
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- VZGDMQKNWNREIO-UHFFFAOYSA-N tetrachloromethane Chemical compound ClC(Cl)(Cl)Cl VZGDMQKNWNREIO-UHFFFAOYSA-N 0.000 description 2
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 2
- UOORRWUZONOOLO-OWOJBTEDSA-N (E)-1,3-dichloropropene Chemical compound ClC\C=C\Cl UOORRWUZONOOLO-OWOJBTEDSA-N 0.000 description 1
- UOCLXMDMGBRAIB-UHFFFAOYSA-N 1,1,1-trichloroethane Chemical compound CC(Cl)(Cl)Cl UOCLXMDMGBRAIB-UHFFFAOYSA-N 0.000 description 1
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 229910021591 Copper(I) chloride Inorganic materials 0.000 description 1
- 238000007399 DNA isolation Methods 0.000 description 1
- 101100369029 Dehalococcoides mccartyi (strain ATCC BAA-2266 / KCTC 15142 / 195) tceA gene Proteins 0.000 description 1
- 101000807980 Dehalococcoides mccartyi (strain VS) Chloroethene reductive dehalogenase Proteins 0.000 description 1
- 241001377006 Dehalococcoides mccartyi CBDB1 Species 0.000 description 1
- 241001119044 Dehalococcoides mccartyi GT Species 0.000 description 1
- 241001235290 Dehalococcoides mccartyi VS Species 0.000 description 1
- 241001509301 Desulfitobacterium dehalogenans Species 0.000 description 1
- 239000005977 Ethylene Substances 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 239000004201 L-cysteine Substances 0.000 description 1
- 235000013878 L-cysteine Nutrition 0.000 description 1
- PLXBWHJQWKZRKG-UHFFFAOYSA-N Resazurin Chemical compound C1=CC(=O)C=C2OC3=CC(O)=CC=C3[N+]([O-])=C21 PLXBWHJQWKZRKG-UHFFFAOYSA-N 0.000 description 1
- ZNOZWUKQPJXOIG-XSBHQQIPSA-L [(2r,3s,4r,5r,6s)-6-[[(1r,3s,4r,5r,8s)-3,4-dihydroxy-2,6-dioxabicyclo[3.2.1]octan-8-yl]oxy]-4-[[(1r,3r,4r,5r,8s)-8-[(2s,3r,4r,5r,6r)-3,4-dihydroxy-6-(hydroxymethyl)-5-sulfonatooxyoxan-2-yl]oxy-4-hydroxy-2,6-dioxabicyclo[3.2.1]octan-3-yl]oxy]-5-hydroxy-2-( Chemical compound O[C@@H]1[C@@H](O)[C@@H](OS([O-])(=O)=O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H]2OC[C@H]1O[C@H](O[C@H]1[C@H]([C@@H](CO)O[C@@H](O[C@@H]3[C@@H]4OC[C@H]3O[C@H](O)[C@@H]4O)[C@@H]1O)OS([O-])(=O)=O)[C@@H]2O ZNOZWUKQPJXOIG-XSBHQQIPSA-L 0.000 description 1
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- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- KRGNPJFAKZHQPS-UHFFFAOYSA-N chloroethene;ethene Chemical class C=C.ClC=C KRGNPJFAKZHQPS-UHFFFAOYSA-N 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
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- OXBLHERUFWYNTN-UHFFFAOYSA-M copper(I) chloride Chemical compound [Cu]Cl OXBLHERUFWYNTN-UHFFFAOYSA-M 0.000 description 1
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- 150000002013 dioxins Chemical class 0.000 description 1
- WZPDTOPYCQOUJT-UHFFFAOYSA-N disodium;sulfide;dihydrate Chemical compound O.O.[Na+].[Na+].[S-2] WZPDTOPYCQOUJT-UHFFFAOYSA-N 0.000 description 1
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- 239000000661 sodium alginate Substances 0.000 description 1
- 235000010413 sodium alginate Nutrition 0.000 description 1
- 229940005550 sodium alginate Drugs 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- IVGPGQSSDLDOLH-UHFFFAOYSA-M sodium;10-oxido-7-oxophenoxazin-10-ium-3-olate Chemical compound [Na+].C1=CC(=O)C=C2OC3=CC([O-])=CC=C3[N+]([O-])=C21 IVGPGQSSDLDOLH-UHFFFAOYSA-M 0.000 description 1
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- 229920000208 temperature-responsive polymer Polymers 0.000 description 1
- 108010029077 tetrachloroethene dehalogenase Proteins 0.000 description 1
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- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D3/00—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances
- A62D3/02—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances by biological methods, i.e. processes using enzymes or microorganisms
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
- C12P5/026—Unsaturated compounds, i.e. alkenes, alkynes or allenes
-
- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
- A62D2101/20—Organic substances
- A62D2101/22—Organic substances containing halogen
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Virology (AREA)
- General Chemical & Material Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Soil Sciences (AREA)
- Mycology (AREA)
- Environmental & Geological Engineering (AREA)
- Toxicology (AREA)
- Business, Economics & Management (AREA)
- Emergency Management (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Processing Of Solid Wastes (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2011020655A JP5711554B2 (ja) | 2011-02-02 | 2011-02-02 | トリクロロエテンの完全脱塩素可能な微生物と当該微生物を用いるトリクロロエテンを含有する汚染物の浄化方法 |
| US13/243,088 US8633008B2 (en) | 2011-02-02 | 2011-09-23 | Bacterium that can perform complete dechlorination of trichloroethene and the method to dechlorinate soils or ground water contaminated with trichloroethene using the bacterium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2011020655A JP5711554B2 (ja) | 2011-02-02 | 2011-02-02 | トリクロロエテンの完全脱塩素可能な微生物と当該微生物を用いるトリクロロエテンを含有する汚染物の浄化方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2012157319A JP2012157319A (ja) | 2012-08-23 |
| JP2012157319A5 JP2012157319A5 (enExample) | 2014-03-06 |
| JP5711554B2 true JP5711554B2 (ja) | 2015-05-07 |
Family
ID=46577682
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011020655A Active JP5711554B2 (ja) | 2011-02-02 | 2011-02-02 | トリクロロエテンの完全脱塩素可能な微生物と当該微生物を用いるトリクロロエテンを含有する汚染物の浄化方法 |
Country Status (2)
| Country | Link |
|---|---|
| US (1) | US8633008B2 (enExample) |
| JP (1) | JP5711554B2 (enExample) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6124558B2 (ja) * | 2012-11-14 | 2017-05-10 | 大阪瓦斯株式会社 | 新規微生物および、鉄シアノ錯体またはニッケルシアノ錯体の分解方法 |
| CN103275992B (zh) * | 2013-01-24 | 2014-11-12 | 南京农业大学 | 溴苯腈还原脱卤酶基因簇bhbA2B2 及其应用 |
| CN112481150B (zh) * | 2020-02-14 | 2022-09-06 | 新加坡国立大学 | 一株降解氯乙烯类有机物的菌株及其在氯乙烯类有机物污染土壤或地下水修复中的应用 |
| CN113025551A (zh) * | 2021-03-26 | 2021-06-25 | 清华大学 | 氯代烃厌氧降解菌剂的制备方法与应用 |
| CN115322917B (zh) * | 2021-05-11 | 2023-10-03 | 中国科学院沈阳应用生态研究所 | 一株一氯甲烷厌氧降解细菌及其在环境生物修复中的应用 |
| CN118324293B (zh) * | 2024-04-01 | 2025-03-18 | 浙江大学 | 一种碳基吸附/微生物厌氧降解风险管控复合材料及应用 |
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| WO2004110933A2 (en) * | 2003-06-10 | 2004-12-23 | Georgia Tech Research Corporation | Dehalococcoides isolate for bioremediation |
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| JP2006042815A (ja) | 2004-07-06 | 2006-02-16 | Kubota Corp | 脱塩素化タンパク質遺伝子、細菌の検出方法、並びにｐｃｅ汚染土壌および汚染水の浄化方法 |
| US8063192B2 (en) * | 2004-08-02 | 2011-11-22 | The Board Of Trustees Of The Leland Stanford Junior University | Microbial reductive dehalogenation of vinyl chloride |
| JP2007089560A (ja) | 2005-09-02 | 2007-04-12 | Tokyo Univ Of Agriculture & Technology | バイオレメディエーションにおける地下水のモニタリング方法 |
| JP2007104916A (ja) | 2005-10-11 | 2007-04-26 | Matsushita Electric Ind Co Ltd | 微生物製剤および揮発性有機化合物浄化方法 |
| JP2010119339A (ja) | 2008-11-19 | 2010-06-03 | Daikin Ind Ltd | 新規なテトラクロロエチレン・デハロゲナーゼ遺伝子（ｒｄｈＡ） |
| WO2011011683A1 (en) * | 2009-07-23 | 2011-01-27 | Arizona Board Of Regents, A Body Corporate Of The State Of Arizona, Acting For And On Behalf Of Arizona State University | Microbial cultures and methods for anaerobic bioremediation |
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