JP5690371B2 - Extract method of Benicus mushroom - Google Patents

Description

  TECHNICAL FIELD The present invention relates to a method for extracting Benix octopus, and particularly relates to a method for extracting Benix octopus that can increase the extraction efficiency of dehydrosulfuric acid.

  Antrodia Camphorata (Antrodia Camphorata) is a rare medicinal material unique to Taiwan. It is only infested on the inner wall of the hollow heartwood of Taiwan's nursery Cinnamomum kanehirai. Benix-no-Mushroom contains abundant triterpenoids, which have functions such as inhibiting cancer cell growth, repairing the liver and promoting liver function, lowering blood lipids and blood pressure, and strengthening immunity.

  Among them, referring to FIG. 1, dehydrosulphurenic acid is a unique triterpenoid component of Benicus mushroom. Dehydrosulfuric acid belongs to the lanostane sterol compound (lanostane), and is an intermediate product of the organism's cells through squalene and sterol in the process of metabolism. Because it easily interferes with biochemical pathways of metabolism and cell membrane formation, it has a significant effect on cell growth cycle and metabolism. In research, dehydrosulfuric acid has the best effect on the treatment of pancreatic cancer and acute myeloid leukemia because it has the effect of promoting cancer cell death and mitotic catastrophe.

  Conventionally, in order to increase the nutritional value of Benix nokitake, the extraction of Benixonotake is performed by eluting the triterpenoids contained in Benix nokitake into the extraction solvent, and then concentrating and drying, and then the Benix nokitake has a high concentration of nutrient components. Earn an extract. The user can obtain the same effect as taking a large amount of Benix octopus by simply taking a small amount of this extract.

  Conventional extraction methods for Benicaria mushrooms can be divided into water extraction methods and organic solvent extraction methods based on the selection of the extraction solvent.

  In the conventional water extraction method, water is used as an extraction solvent, and a single herb extract of Benix octopus is obtained by extracting from one herb.

  In addition, as a conventional organic solvent extraction method, for example, an organic solvent such as alcohol, ester, alkanes and alkyl halides is used as an extraction solvent, and the above herbaceous material of Benix nokitake is soaked, and then the above-mentioned organic solvent is extracted at low temperature. By removing the solvent by distillation, one extract of Benicus mushroom is obtained.

  In addition, in the invention disclosed in the Republic of China No. I335314, the extract of Benix nokitake extracted with an ethanol solvent contains the contents of antroquinonol B (antroquinonol B) and antroquinonol C (antroquinonol C). It is described that relatively high compounds are included.

Republic of China Notice No. I335314

  In the conventional water extraction method, since the water solubility of triterpenoids is not good, there is a problem that the total content of triterpenoids contained in the water extract of Benix nokitake is not universally high.

  In addition, in the conventional organic solvent extraction method, compared with the water extraction method, the total content of extracted triterpenoids is relatively high, but the extraction amount of dehydrosulfuric acid cannot still be increased effectively, There was a problem that the extraction efficiency of dehydrosulfuric acid was poor.

  The present invention has been devised in view of such problems. The first object of the present invention is to increase the elution rate of dehydrosulfuric acid, thereby increasing the content of dehydrosulfuric acid. It is an object of the present invention to provide a method for extracting Benixo mushroom that can obtain an extract of camphor mushroom.

  In order to achieve the above-mentioned object, the method for extracting Benix octopus bamboo according to the present invention is impregnated by impregnating the herbaceous material of Benix octopus into a salt solution and absorbing the salt solution into the herbicide of Benix octopus The method includes the steps of obtaining a venomus mushroom, and obtaining an extract of the venix mushroom by immersing the impregnated mushroom in an alcohol solvent and passing through ultrasonic vibration.

In addition, according to the present invention, there is provided a method for extracting the venix mushroom, which is placed in one sealed space before the impregnated venom mushroom is soaked and extracted with the alcohol solvent, and the temperature is 110 to 150 ° C. The step of heating and steaming in an environment with a pressure of 1 to 3 kg / cm ² may be included. Alternatively, first, impurities on the surface of the herbaceous material of Benix nocturnus can be removed, and then the herbicide material of Benicus nokitake can be impregnated again in the salt solution. Further, the heating and cooking time may be 15 to 60 minutes. Further, it can be cooked by heating in an environment where the temperature is 121 ° C. and the pressure is 1 kg / cm ² . The salt solution can also be a 2% sodium chloride solution. It is also possible to impregnate 500 grams of Benix nokitake herbal medicine into 1 liter of sodium chloride solution. The alcohol solvent may be a 95% ethanol solvent. Moreover, the herbicide material of the above-mentioned Benixonochatake can also be selected from the fruit body of Benixonochatake.

  According to the present invention, by increasing the efficiency of extraction of dehydrosulfuric acid, there is an advantage that it is possible to obtain an extract of Benix octopus having a high content of dehydrosulfuric acid.

FIG. 1 is a diagram showing the chemical formula of dehydrosulfuric acid. FIG. 2A is a diagram showing the results of analysis by high performance liquid chromatograph of extracts of the A1 set of Benix nokitake. FIG. 2B is a diagram showing the results obtained by analyzing high-performance liquid chromatographs of the extract of the A2nd set of Benix nokitake. FIG. 2C is a diagram showing the results of analysis by high performance liquid chromatograph of extracts of A3rd set of Benix nokitake. FIG. 2D is a diagram showing the results of analyzing the extract of A4th set of Benix nokitake by high performance liquid chromatography. FIG. 2E is a diagram showing the results of analyzing the extract of the A5th set of Benix nokitake with a high performance liquid chromatograph.

  Embodiments of the present invention will be described below with reference to the drawings.

  The method for extracting Benixonocha mushroom according to the present invention comprises impregnating a herbicide material of Benixnochatake into a salt solution, and absorbing the salt solution into the herbicide material of Benixnochatake, thereby obtaining the impregnated Benicus mushroom. Immersing the impregnated venomus mushroom in an alcohol solvent and subjecting it to ultrasonic vibration to obtain an extract of the venix mushroom.

  More specifically, impregnated venix mushrooms are obtained by impregnating a fixed amount of a herb material of venix mushrooms in a fixed amount of salt solution. Among them, in this embodiment, first, impurities on the surface of the herbaceous material of Benix nocturnus are removed, and then impregnated in the salt solution again, so that the impurities on the surface of the herbicide material of Benicus nokitake An adverse effect on the subsequent extraction effect can be avoided. The salt solution can be selectively made of sodium chloride having a weight percentage of 2%. Since sodium chloride is easy to acquire and easy to price, the cost can be kept low. Since the performance of the drug can be changed after the drug is manufactured through saline, the action of the drug can be enhanced.

  In this embodiment, the above-mentioned medicinal material of Benix nokitake is impregnated in the sodium chloride solution, and preferably by impregnating it in one sealed container, external impurities are contained in the sodium chloride solution. You can avoid entering. Thus, the impregnated venix mushroom can be formed jointly by sealing and soaking into the venix mushroom through the sodium chloride solution. For example, in this example, 500 grams of Benix nokitake herbal medicine is selectively impregnated in the 1 liter sodium chloride solution for 24 hours after removing surface impurities. , To acquire the impregnated venix mushroom.

  Next, an impregnated venix mushroom is immersed in an alcohol solvent and subjected to ultrasonic vibration to obtain an extract of venix mushroom. In this embodiment, the alcohol solvent can be selectively composed of an ethanol solvent having a concentration of 95%, and a triterpenoid having a relatively high content can be extracted. Furthermore, the ethanol solvent can be formed in a crystalline form with an inorganic component, and the solubility can be increased with respect to a molecular compound having a high water solubility.

  Preferably, 5 grams of the impregnated Benix octopus is placed in 600 milliliters of the ethanol solvent and extracted by oscillating with an ultrasonic wave having a frequency of 40 KHz in an environment of 25 ° C. The active ingredient (for example, triterpenoid) can be eluted in the ethanol solvent. Further, preferably, the extraction time is 8 hours, and the extraction of Benicus mushroom can be obtained by increasing the total extraction amount by repeating the extraction three times. In addition, one concentrated extract of Benicaria mushroom is obtained by going through freeze-drying (-55 ° C., vacuum degree 0 Pa (0 torr)) again.

More preferably, in the method for extracting Benicobacterium of the present invention, before the impregnated Benicobacter mushroom is further extracted by immersing it in an alcohol solvent, the impregnated Benicocidae is placed in one sealed space, and the temperature is 110 to 150. The elution of dehydrosulfuric acid can be promoted by including a step of heating and cooking in an environment where the pressure is from 1 to 3 kg / cm ² up to ° C. More specifically, in this embodiment, by providing the sealed space using an apparatus or equipment (for example, a pressure cooker) that can selectively control a high temperature and high pressure environment, Control the temperature.

In the present embodiment, the impregnated venix mushroom is placed in a pressure cooker capable of controlling the high temperature and high pressure environment, and the temperature is controlled from 110 to 150 ° C., and the pressure is controlled from 1 to 3 kg / cm ^2. Cook for 15 minutes to 1 hour. Among them, if the temperature is lower than 110 ° C., the efficiency of extraction of triterpenoids from Benix nokitake falls. If the temperature is higher than 150 ° C., there is a risk of destroying the active ingredient in Benicus mushroom due to the high temperature.

And when it is going to reach a high temperature from 110 ° C. to 150 ° C., an environment with a pressure of 1 to 3 kg / cm ² must be provided through the enclosed space. If the pressure is lower than 1 kg / cm ² , the steam will not easily rise to the required temperature. If the pressure is higher than 3 kg / cm ² , the steam temperature may become too high. For example, in this example, the color of the appearance of the impregnated venix mushroom is deepened and becomes black red by steaming from 15 minutes to 60 minutes at 121 ° C. and 1 kg / cm ² environmental conditions. The steamed impregnated bamboo shoots can be obtained, and then the extract of venix bamboo shoots can be efficiently obtained by soaking and extracting again with the alcohol solvent.

  As described above, steaming does not leak to the outside by steaming in the sealed space, so that not only the temperature and pressure are easily controlled, but also the high pressure environment is provided by the sealed space. Since the boiling point of water can be increased, the temperature of the steam can be further increased to 110 ° C. or higher. In this way, by steaming the impregnated venix mushroom with high-temperature and high-pressure steam in the sealed space, the impregnated venix mushroom can be cooked in a short time until the surface becomes black-red. Therefore, the cooking time can be greatly shortened, the efficiency can be further increased, and the cost can be kept low.

  In order to prove that the extraction method of Benixo nokitake of this example can increase the content of dehydrosulfuric acid in the above extract of Benixonotake, this example was analyzed by high performance liquid chromatograph (HPLC). I did it.

  More specifically, in this test, for example, an extract of Benicus mushroom shown in Table 1 could be obtained. Group A1 is an extract of Benix nokitake obtained by soaking and extracting the above-mentioned ethanol solvent from a herb of Benix nokitake, and Group A2 is an extract of Benix nokitake obtained by soaking and extracting from the above-mentioned ethanol solvent. Extracts A3 to A5 are extracts of Benicus mushrooms obtained by steaming 15, 30 and 60 minutes of impregnation Benicus mushrooms, respectively, and then immersing and extracting with ethanol solvent. Then, 0.2 g each of the above-mentioned Benix nokitake extract was weighed and put into one spiral test tube, 5 ml of methanol was added, and the mixture was vibrated with ultrasonic waves for 15 minutes, and then 3,000 rpm for 10 minutes. After centrifuging for 5 minutes, 5 milliliters of the supernatant was taken and placed in a clean test tube, and then water bathed again at 100 ° C. and heated to dryness.

  Then, analysis was performed selectively using a cylinder of Purospher STAR (Merck) RP-18e (5 μm) 250 mm × 4.6 mm, and acetonitrile (Acetonitrile, hereinafter referred to as ACN) shown in Table 2 and 0.085. Subsequent analysis was performed with the eluate of a phosphoric acid solution at% as the mobile phase, the flow rate was 1 ml / min, and the measurement wavelength was 254 nm.

  2A to 2E and Table 3, when compared with the A1 set, Benix nokitake herb crude material obtained after the impregnation with the sodium chloride solution (A2 set) and through the soaking extraction with the ethanol solvent. Among the extracts of camphor mushrooms, there are antcin K, antcin C, zhankuic acid C, zhankuic acid A and dehydroeburicoic acid. Although all the extraction amounts had a phenomenon of reduction, it was found that the extraction amount of dehydrosulphurenic acid increased about 4 times compared to the A1 group.

  In addition, the amount of dehydrosulfuric acid extracted from the extract (A3 set) of Benix octopus obtained after impregnation with the sodium chloride solution, further steaming for 15 minutes, and soaking and extracting with the ethanol solvent Increased, 6 times that of the A1 group. When the cooking time was extended by 30 minutes (A4 set), the amount of dehydrosulfuric acid extracted was 5.5 times that of A1 set. It was found that when the cooking time was 60 minutes (A5 group), the amount of dehydrosulfuric acid extracted was three times that of A1 group.

  As can be seen here, in the extraction method of Benicus mushrooms of this example, the extraction amount of dehydrosulfuric acid can be obviously increased by impregnation with the above-mentioned salt solution, and further steaming at high temperature and high pressure can be performed. It has been found that the extraction efficiency of dehydrosulfuric acid can be further enhanced by performing the cooking, and the cooking time is preferably 15 minutes.

  In combination, according to the method of extracting Benix nokitake of the present invention, after the impregnation with the salt solution, dehydrosulfuric acid can increase the extraction efficiency in the alcohol solvent, and the content of dehydrosulfuric acid is further increased. Achieving the objective of obtaining a relatively high extract of Benicus mushroom.

  The present invention may be implemented in other ways without departing from the spirit and essential characteristics thereof. Accordingly, the preferred embodiments described herein are exemplary and not intended to be limiting.

Claims (8)

By impregnating the herbaceous material of Benix nokitake into a salt solution and absorbing the salt solution into the herbicide material of the above-mentioned Benix nokitake, the step of obtaining the impregnated Benicus nokitake and immersing the above impregnated Benicus nokitake in an alcohol solvent by going through the ultrasonic vibration, seen including a step of obtaining an extract of Antrodia camphorata, the saline solution is 2% sodium chloride solution, extraction method Antrodia camphorata. In addition, before soaking and extracting the above impregnated venomus mushrooms with the above alcohol solvent, the above impregnated venix mushrooms are placed in one sealed space, the temperature is 110 to 150 ° C., and the pressure is 1 to 3 kg / cm ^2. The method for extracting Benicaria mushrooms according to claim 1, comprising the step of cooking under steaming.   First, after removing impurities on the surface of the herbaceous material of Benixonochatake, the herbicide of Benixnochatake is impregnated again in a salt solution. Extraction method. The method for extracting Benicaria mushrooms according to claim 2, wherein the heating and steaming time is 15 to 30 minutes. 5. The method for extracting Benicaria mushrooms according to claim 2, wherein heating and steaming are performed in an environment of a temperature of 121 ° C. and a pressure of 1 kg / cm ² . 2. The method of extracting Benicaria mushrooms according to claim 1 , wherein 500 g of Benicorella mushroom herbal medicine is impregnated in 1 liter of sodium chloride solution.   3. The method of extracting Benicus mushroom according to claim 1 or 2, wherein the alcohol solvent is a 95% ethanol solvent.   3. The method of extracting Benix octopus mushrooms according to claim 1 or 2, wherein the herbaceous material of Benix octopus is selected from the fruit bodies of Benix octopus.

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