JP5669843B2 - 改良型単一散乱モード検出を有する、複雑流体の動的光散乱型マイクロレオロジー - Google Patents
改良型単一散乱モード検出を有する、複雑流体の動的光散乱型マイクロレオロジー Download PDFInfo
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Description
本発明は、コロイド複雑流体や複雑生体液のような、複雑流体の粘弾性パラメータを取得するための方法および装置に関する。
[発明の背景]
粘弾性とは、1つの素材において、粘性的性質と弾性的性質とが同時に存在することを意味する。複雑流体及び構造化された流体の多くは、粘弾性特性を示し、つまり、弾性体のようにエネルギーを蓄積可能であることに加え、粘性流体のようにエネルギーを消散することも可能である。圧力がそういった粘弾性流体に付与されると、粘弾性流体は、全エネルギー入力を熱として消散する代わりに、エネルギー入力の一部を蓄積する。また、圧力が取り除かれると、粘弾性流体のひずみの一部が回復する。
・系が単一散乱を示す。系で多重散乱が発現すると、この解析はもはや有効ではない。
・全原理が、埋め込まれたプローブ粒子の運動への追従に基づくため、散乱は、埋め込まれたプローブ粒子による影響が最も大きい。
[発明の概要]
本発明のいくつかの態様が、本出願にて開示される。
本発明による装置は、散乱光が、173度から13.5度までのような、様々な異なる角度にわたって検出されるように、構成可能である。また、装置は、周波数応答の領域を伸長するために、後方散乱モードまたは透過モードの両方で測定できるように、構成可能である。これらの課題は、1つの検出器が移動できるようにする、または2つ以上の検出器を供給する、など様々な方法で達成可能である。また、取得した周波数を伸長し、および/または必要なプローブ粒子の量を調整することで多重散乱を最小限に抑えるために、30ナノメートルから1マイクロメートルまでに渡る多くの異なるプローブサイズを利用した測定が可能である。更に、対象の複雑流体との相互作用を最小限に抑えるために、多くの異なるプローブ化学を利用した測定も実施可能である。
上記のアプローチを実証するために、DLS型光マイクロレオロジーが、ゼータサイザーナノ(Malvern Instruments Limited)で、ハードウェアを変更することなく実施された。ゼータサイザーナノが、NIBS式手法を実行するように設計されている点は、注目すべきである。例えば、ゼータサイザーナノは、本出願が援用する、米国仮出願番号61/206,688に記載されている。
Claims (9)
- マイクロレオロジー測定方法であって、
プローブ粒子を複雑流体試料に埋め込む工程と、
前記複雑流体試料をコヒーレント光で照射する工程と、
前記複雑流体試料中のプローブ粒子によるコヒーレント光の散乱から後方散乱光子を検出する工程であって、該検出は、前記検出される後方散乱光子における多重散乱光の影響を実質的に低減するように、前記コヒーレント光の光軸に十分に近接し、かつ、前記複雑流体試料に十分に近接した位置から行われる、工程と、
前記検出された後方散乱光子を表す検出信号に相関演算を実行する工程と、
前記相関演算の結果から、前記複雑流体試料の少なくとも1つのレオロジー特性を導出する工程と、
を有するマイクロレオロジー測定方法。 - 請求項1に記載の方法であって、
前記相関演算は自己相関演算である、
マイクロレオロジー測定方法。 - 請求項1又は2に記載の方法であって、
検出の工程は、さらに、光周波数応答の領域を伸長するために、前方透過モードにおいてプローブ粒子により散乱されたコヒーレント光を検出する工程を含む、
マイクロレオロジー測定方法。 - 請求項3に記載の方法であって、
散乱光は、173度から13.5度までにわたる様々な異なる角度で検出され、前記相関演算実行の工程、および前記導出の工程は、前記様々な角度で検出された散乱光に実行される、
マイクロレオロジー測定方法。 - 請求項1〜4の何れか1項に記載の方法であって、
取得される周波数を伸長するために、および/または必要なプローブ粒子の量を調整することで多重散乱を最小限に抑えるために、検出の工程は、30ナノメートルから1マイクロメートルまでにわたる様々な異なるプローブサイズを利用して実行される、
マイクロレオロジー測定方法。 - 請求項1〜5の何れか1項に記載の方法であって、
検出の工程は、前記複雑流体試料との相互作用を最小限に抑えるように選択された様々な異なるプローブ化学を利用して実行される、
マイクロレオロジー測定方法。 - 請求項1〜6の何れか1項に記載の方法であって、
前記複雑流体試料から受けた散乱光を第1および第2部分に分割する工程と、
前記散乱光の前記第1部分からの光子を検出する工程と、
前記散乱光の前記第2部分からの光子を検出する工程と、を有し、
前記相関演算は、前記第1部分における前記後方散乱光子を表す第1検出信号と、前記第2部分における前記後方散乱光子を表す第2検出信号との間での相互相関演算であり、前記レオロジー特性は、粘弾性または粘性である、
マイクロレオロジー測定方法。 - 請求項1〜7の何れか1項に記載の方法であって、
前記複雑流体試料は、1.5ミリメートル以下の光路長を介してコヒーレント光で照射され、前記レオロジー特性は、粘弾性または粘性である、
マイクロレオロジー測定方法。 - 請求項1〜8の何れか1項に記載の方法であって、
前記プローブ粒子は、プローブ粒子による散乱が優位となるような濃度で前記複雑流体試料に埋め込まれる、
マイクロレオロジー測定方法。
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US27448009P | 2009-08-17 | 2009-08-17 | |
US61/274,480 | 2009-08-17 | ||
PCT/GB2010/051354 WO2011021032A1 (en) | 2009-08-17 | 2010-08-17 | Dynamic light scattering based microrheology of complex fluids with improved single-scattering mode detection |
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CN103776802B (zh) * | 2014-01-10 | 2016-06-08 | 上海理工大学 | 测量粘弹性流体的微流变测量装置及方法 |
CN103884713B (zh) * | 2014-01-14 | 2016-08-31 | 西华大学 | 聚合物溶液变形迟缓时间常数测定方法 |
GB2533602B (en) | 2014-12-23 | 2020-11-11 | Jemella Ltd | Method and apparatus for manipulating the shape of hair |
DE102015103139B3 (de) * | 2015-03-04 | 2016-08-11 | Aiq Dienstleistungen Ug (Haftungsbeschränkt) | Verteilte optische Messvorrichtungen und Verfahren zum Ausführen einer Messung |
GB2537550A (en) * | 2015-07-13 | 2016-10-19 | Malvern Instr Ltd | Dynamic light scattering based optical microrheology in non-aqueous solutions |
US11273124B2 (en) * | 2019-05-23 | 2022-03-15 | Brown University | Antifungal nanoparticles for targeted treatment of fungal infections |
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