JP5646277B2 - Disinfectant and acne treatment / prevention agent - Google Patents

Description

The present invention relates to a fungicide and a therapeutic / preventive agent for acne.

In recent years, the use of antibacterial materials and bactericides for preventing various problems due to the growth of bacteria has been increasing. In particular, due to the epidemic of new influenza, it is desired to prevent the spread of disease by using a bactericidal skin external preparation, and to obtain a deodorizing / deodorizing effect, bactericidal properties having excellent performance・ Development of antibacterial skin external preparation is desired.

As a skin external preparation having bactericidal properties that are widely used at present, those containing alcohol compounds such as ethanol can be mentioned. Since ethanol is a cheap and highly safe compound, it is widely used as a disinfectant and also widely used in cosmetics. In addition, alkanediols such as pentylene glycol, hexanediol, and caprylyl glycol, and chlorine compounds such as ethyl paraoxybenzoate and benzalkonium chloride are also used.

On the other hand, if there is a bactericidal agent that can obtain stable bactericidal properties by blending in water in a trace amount, bactericidal properties can be imparted in cosmetics having various compositions. Moreover, when ethanol is applied to the skin, a refreshing sensation is felt at the time of transpiration, but there is a consumer who does not like such a sensation, so a bactericidal agent containing a bactericidal component other than ethanol is required.

About gold ion water, it describes in patent document 1 with the manufacturing method. However, the sterilization performance of gold ions is not described.

Patent Document 2 describes a nanoparticle containing a functional group in which a metal ion is reversibly bonded to the particle surface, and describes the use of such a particle as an antibacterial agent. . However, since such particles do not necessarily have a high elution efficiency of gold ions, they must be added in a high amount in the composition. For this reason, since gold ions colloidally form colloidal particles, problems such as discoloration occur, so that they are not easy to use in the cosmetics field.

Patent Document 3 describes a skin external preparation containing an antibacterial ceramic carrying an antibacterial metal, and describes that it can be used as a skin external preparation for acne treatment. However, it is not described that gold ions themselves are blended, but only that a ceramic carrying a metal is blended. Such an external preparation for skin is not necessarily good in the elution efficiency of metal ions, so that it is necessary to increase the amount of the compound, which causes a problem such as discoloration.

Patent Document 4 describes the use of a metal ion as a complex with an organic chelate compound as a disinfectant. However, the complex with the organic chelate compound is an insoluble substance, and the elution efficiency of metal ions is poor. Therefore, while ensuring sufficient bactericidal properties in the use of external skin preparations and household bactericides, discoloration / It is difficult not to cause problems such as alteration.

Japanese Patent Laid-Open No. 2004-182,500 JP-T-2004-526566 JP 7-109217 A Special table 2003-521472 gazette

This invention is made | formed in view of such a situation, Comprising: It aims at providing the bactericide which has high safety | security and the outstanding bactericidal performance, and the therapeutic / preventive agent of acne.

The present invention is a bactericide containing gold ions as an active ingredient.
It is preferable that the said bactericidal agent is used for sterilizing acne bacteria.
The present invention is also a therapeutic / preventive agent for acne characterized by containing gold ions.

INDUSTRIAL APPLICABILITY According to the present invention, it is possible to provide a bactericidal agent and an acne treatment / prevention agent that are excellent in safety, do not cause problems such as discoloration, and exhibit high bactericidal performance even at low concentrations.

The present inventors conducted experiments on superoxide scavenging ability and bactericidal properties for aqueous solutions containing gold ions, thereby clarifying that gold ions have high superoxide scavenging ability and bactericidal performance. Is completed. In particular, in terms of function as a bactericidal agent, it is excellent in bactericidal performance against acne bacteria, and thus can be used as a therapeutic / preventive agent for acne.

Furthermore, it can also be used as a bactericidal component blended in a bactericide composition for the purpose of bactericidal / deodorizing. In recent years, spraying a bactericidal composition on a curtain, stuffed animal, bedding, carpet, etc. for the purpose of deodorization is often performed at home. It is desired that the bactericidal composition used for such applications is water-soluble and does not cause discoloration or material alteration after spraying. Gold ions can also be used as an active ingredient in a fungicide composition for such applications. In particular, it is ionic and easy to mix in an aqueous composition, and exhibits high sterilization performance at a low concentration. Therefore, it has advantages such as being less likely to cause problems such as discoloration and alteration in the object to be sterilized. Have.

Further, it has been revealed that even if the gold ion water concentration is relatively low at 50 ppm or less, it has sufficient sterilization performance. Therefore, no discoloration occurs due to colloidalization of gold ions, which is advantageous in terms of cost. Such a bactericidal agent can be blended in skin preparations such as cleaning agents, cosmetics, acne prevention / treatment agents, various foods, and the like.

The disinfectant of the present invention is a method for use in an aqueous composition such as cosmetics and foods and used as a preservative to prevent its decay; prevention and treatment of acne by disinfecting acne bacteria resident on the skin There are uses such as a method of blending and using as an active ingredient in an external preparation for skin; a method of using as an external preparation for skin for sterilizing fingers and the like.

Furthermore, according to the experiments of the present inventors, gold ions have a superoside anion scavenging action even at a concentration as low as 0.3 ppm, compared with known superoxide scavengers such as ascorbic acid and theaflavin. It was also revealed that it has an excellent superoxide anion scavenging action at a low concentration. Since it exhibits a superoxide anion erasing action in such a small amount, it can be stably blended into the composition, and it is difficult to cause a problem of discoloration due to alteration and is advantageous in terms of cost. It is.

Furthermore, the superoxide anion scavenger of the present invention can be used not only in skin external preparations such as cosmetics but also in pharmaceuticals, health foods and the like.

The form of the bactericidal agent of the present invention is not particularly limited, and is not particularly limited as long as gold ions can be stably present, and may be an aqueous composition such as an aqueous solution or an O / W emulsion. preferable.

It does not specifically limit as said gold ion water, For example, it can obtain by the following methods as described in the said patent document 1. First, in a state where a gold solution in which gold is dissolved in aqua regia (hydrochloric acid 3: nitric acid 1) is put in a high heat resistant container such as a crucible or an evaporating dish, the high heat resistant container is heated and the gold dissolved liquid is heated in a high heat resistant container. Evaporate to dryness at the same time and volatilize hydrochloric acid and nitrogen oxides.

Next, after cooling the heat-resistant container to room temperature, dissolve the precipitate deposited in the heat-resistant container with aqua regia, heat the heat-resistant container again, and evaporate the evaporated dry solid solution in the heat-resistant container. At least one re-evaporation to dryness step of evaporating and evaporating chlorine and nitrogen oxides at the same time is carried out.
Then, after the re-evaporation drying process is completed, the high heat resistant container is cooled to room temperature, purified water is poured into the high heat resistant container, and the final precipitate remaining in the high heat resistant container is dissolved in the purified water to obtain gold ion water. Get.

In the disinfectant of the present invention, the gold ions are preferably added at a concentration of 0.0001 to 250 ppm, more preferably 0.001 to 150 ppm, and more preferably 10 to 60 ppm with respect to the total amount of the composition. Is more preferable. By being within the above range, suitable sterilization performance can be obtained, and discoloration of the solution and cost increase due to excessively high concentration can be avoided.

The bactericidal agent of the present invention is expected to have an effect on acne, Escherichia coli, Pseudomonas aeruginosa, MRSA, Candida, ringworm, black skin gourd, Staphylococcus aureus, virus and the like.

When gold ions are used as a superoxide anion scavenger, the gold ions are preferably added at a concentration of 0.0001 to 50 ppm, more preferably 0.001 to 10 ppm, based on the total amount of the composition. Preferably, it is 0.001 to 5 ppm. By being within the above range, suitable sterilization performance can be obtained, and discoloration of the solution and cost increase due to excessively high concentration can be avoided.

The above-described superoxide anion scavenger and gold ion having a function as a bactericidal agent can be suitably used as a compounding component of a skin external preparation. When blended into an external preparation for skin, it contains gold ions, so it has an excellent bactericidal action against bacteria that are present on the skin and adversely affect health and beauty. Can be used as Gold ions can also be added for the purpose of preventing decay of the external preparation for skin.

The skin external preparation containing gold ions is not particularly limited, and is a cosmetic for hair such as lotions, emulsions, creams, gels, shampoos, body soaps, hand soaps, soaps, rinses, treatments, set agents, etc. Can be listed. Moreover, the thing of forms, such as the sheet-like cosmetics which consist of these skin external preparations, and the sheet-like cosmetics which impregnated these skin external preparations in base materials, such as a nonwoven fabric, can also be mentioned.

For the above-mentioned external preparation for skin, oils, powders (pigments, resin particles), surfactants, adhesives, resins, water-based colorants, preservatives, fragrances, ultraviolet absorbers (organic, Inorganic, including UV-A and B), solvent, natural plant extract, seaweed extract, herbal medicine, polyhydric alcohol and other moisturizers, salts, antioxidants Components such as an agent, a neutralizing agent, a pH adjusting agent, and an insect repellent can be appropriately blended.

Examples of the oil agent include volatile and non-volatile oil agents usually used in cosmetics, solvents, resins, and the like, and may be liquid, paste, or solid at room temperature. Examples of oils include, for example, higher alcohols such as isostearyl alcohol, lauryl alcohol, hexadecyl alcohol, octyldodecanol; fatty acids such as isostearic acid, undecylenic acid, oleic acid; isononyl isononanoate, isotridecyl isononanoate, myristyl myristate, Esters such as hexyl laurate, decyl oleate, isopropyl myristate, hexyl decyl dimethyloctanoate, glyceryl monostearate, diethyl phthalate, ethylene glycol monostearate, octyl oxystearate, diisostearyl malate; liquid paraffin , Hydrocarbons such as paraffin, petrolatum, squalane; lanolin, reduced lanolin, liquid lanolin, carnauba wax, candelilla wax, ceresin, ozokerai , Waxes such as microcrystalline wax, polyethylene wax, ethylene-alpha-olefinic co-oligomer, ethylene propylene polymers, and the like.

Examples of powders include red 104, red 102, red 226, red 201, red 202, yellow 4, black 401, etc .; blue 1 aluminum lake, yellow 4 aluminum lake, Lake pigments such as yellow No. 5 aluminum lake and yellow No. 203 barium lake; nylon powder, silk powder, urethane powder, Teflon (registered trademark) powder, silicone powder, polymethyl methacrylate powder, cellulose powder, silicone elastomer spherical powder, Polymers such as polyethylene powder, yellow iron oxide, red iron oxide, black iron oxide, chromium oxide, carbon black, colored pigments such as ultramarine and bitumen; white pigments such as zinc oxide, titanium oxide and cerium oxide; talc, mica and sericite , Kaolin, plate-like barium sulfate and other extender pigments; mica tita Pearl pigments such as; metal salts such as barium sulfate, calcium carbonate, magnesium carbonate, aluminum silicate, magnesium silicate; inorganic powders such as silica and alumina, bentonite, smectite, boron nitride, fine particle titanium oxide, fine particle zinc oxide, etc. These are also conventionally known surface treatments such as N-acylated lysine treatment, amino acid treatment, hydrophilic polymer treatment, oil treatment, silicone treatment, metal soap treatment, inorganic compound treatment, plasma treatment, mechanochemical treatment, etc. It is also possible to use the one with the mark. There are no particular restrictions on the shape of these powders (spherical, rod-like, needle-like, plate-like, indeterminate, flake-like, spindle-like, etc.). The size of the powder is preferably in the range of 5 nm to 100 μm, more preferably 10 nm to 25 μm. These powders may be processed alone or may form a mixture and be processed together. Moreover, it is also possible to process what adjusted the color of the mixture to skin color etc. Furthermore, it is possible to obtain a treated powder having an ultraviolet protection function by using an ultraviolet scattering component such as fine particle titanium oxide or fine particle zinc oxide.

As the surfactant, for example, an anionic surfactant, a cationic surfactant, a nonionic surfactant, and an amphoteric surfactant can be used. More specifically, fatty acid soap, α-acyl sulfonate, N-acyl amino acid salt, alkyl sulfonate, alkyl allyl sulfonate, alkyl naphthalene sulfonate, alkyl sulfate, alkyl ether sulfate, alkyl amide sulfate, Anionic surfactants such as alkyl phosphates, alkylamide phosphates, alkyloyl alkyl taurates, sulfosuccinates, perfluoroalkyl phosphates, alkyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium bromide , Cationic surfactants such as cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, behenyltrimethylammonium bromide, Nonionic surfactants such as acid alkanolamide, POE (polyoxyethylene, the same shall apply hereinafter) sorbitan fatty acid ester, POE glycerin fatty acid ester, POE fatty acid ester, polyether-modified silicone, carboxybetaine type, amide betaine type, sulfobetaine type And amphoteric surfactants such as hydroxysulfobetaine type, amide sulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type, and amidoamine type. Natural surfactants such as saponins and sugar surfactants can also be used.

Examples of the preservative include benzoic acid, sodium benzoate, undecylenic acid, salicylic acid, sorbic acid or a salt thereof, dehydroacetic acid or a salt thereof, methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, isopropyl paraoxybenzoate, Organic acids such as butyl paraoxybenzoate, isobutyl paraoxybenzoate, and benzyl paraoxybenzoate and their derivatives, isopropylmethylphenol, chlorhexidine gluconate, cresol, thymol, parachlorophenol, phenylethyl alcohol, phenylphenol, sodium phenylphenol, phenoxyethanol , Phenols such as phenoxydiglycol, phenol, benzyl alcohol, stearyltrimethylammonium chloride, cetyl chloride Quaternary ammonium salts such as limethylammonium, cetylpyridinium chloride, benzalkonium chloride, benzethonium chloride, methylbenzethonium chloride, lauryltrimethylammonium chloride, alkylisoquinolium bromide, domifenone bromide, tea extract, hinokitiol, apple extract, etc. Plant extract, chloramine T, chlorhexidine, zinc pyrithione and the like.

Examples of organic ultraviolet absorbers include 2-methoxyhexyl paramethoxycinnamate, 2-ethylhexyl paradimethylaminobenzoate, 2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfate, 2,2′-dihydroxy-4-methoxybenzophenone, p-methoxyhydrocinnamic acid diethanolamine salt, paraaminobenzoic acid (hereinafter abbreviated as PABA), homomenthyl salicylate, methyl-O-aminobenzoate, 2-ethylhexyl-2-cyano -3,3-diphenyl acrylate, octyl dimethyl PABA, octyl salicylate, 2-phenyl-benzimidazole-5-sulfuric acid, triethanolamine salicylate, 3- (4-methylbenzylidene) camphor, 2,4-dihydroxybenzene Zophenine, 2,2 ′, 4,4′-tetrahydroxybenzophenone, 2,2′-dihydroxy-4,4′-dimethoxybenzophenone, 2-hydroxy-4-N-octoxybenzophenone, 4-isopropyldibenzoylmethane, Examples include butylmethoxydibenzoylmethane, 4- (3,4-dimethoxyphenylmethylene) -2,5-dioxo-1-imidazolidinepropionate 2-ethylhexyl, and the like.

Examples of the solvent include purified water, cyclic silicone, ethanol, light liquid isoparaffin, lower alcohol, ethers, LPG, fluorocarbon, N-methylpyrrolidone, fluoroalcohol, and next-generation chlorofluorocarbon.

Examples of natural plant extract ingredients, seaweed extract ingredients, and herbal medicine ingredients are Ashitaba extract, Avocado extract, Achacha extract, Altea extract, Arnica extract, Aloe extract, Apricot extract, Apricot extract, Ginkgo biloba extract, Fennel extract, Turmeric Extract, Oolong tea extract, Ages extract, Echinacea leaf extract, Ogon extract, Oat extract, Auren extract, Barley extract, Hypericum extract, Oyster extract, Dutch mustard extract, Orange extract, Seawater dried product, Seaweed extract, Hydrolyzed elastin, Hydrolysis Wheat powder, hydrolyzed silk, chamomile extract, carrot extract, cormorant extract, licorice extract, calcade extract, oyster extract, kiwi extract, kina extract, cucumber extract, guanosine, gardenia Kiss, Kumazasa extract, Clara extract, Walnut extract, Grapefruit extract, Clematis extract, Chlorella extract, Mulberry extract, Gentian extract, Tea extract, Yeast extract, Burdock extract, Rice bran extract, Rice germ oil, Comfrey extract, Collagen, Cowberry extract Extract Extract, Horsetail extract, Horseshoe extract, Hawthorn extract, Elderberry extract, Yarrow extract, Japanese peppermint extract, Sauce Extract, mallow extract, senkyu extract, assembly extract, soybean extract, lysium extract, thyme extract, tea extract, clove extract, chigaya extract, chimpi extract, touki extract, tofu extract, tonin extract, spruce extract, dokudami extract, tomato extract, natto extract, Carrot extract, garlic extract, wild rose extract, hibiscus extract, bacmond extract, lotus extract, parsley extract, honey, hamamelis extract, parietaria extract, toad extract, bisabolol, loquat extract, dandelion extract, burdock extract, butterfly extract, butcher bloom extract, Grape extract, propolis, loofah extract, safflower extract, peppermint extract, bodaiju extract, button extract, hop exhaust Pine extract Examples include rose extract, rosemary extract, Roman chamomile extract, royal jelly extract and the like.

Examples of water-based colorants include naturally occurring pigments such as yellow No. 4, yellow No. 5, blue No. 1, blue No. 2, red No. 226, caramel, copper chlorophyll, gardenia, turmeric, safflower and the like.

As the polyhydric alcohol, any component widely used as a cosmetic material can be used. For example, glycerin, diglycerin, ethylene glycol, diethylene glycol, triethylene glycol, propylene glycol, dipropylene glycol, polyethylene glycol , Polypropylene glycol, 1,3-butylene glycol, 3-methyl-1,3-butanediol, 1,2-pentanediol, polyglycerol and derivatives thereof, polyoxyethylene glycerol (26E.O), sorbitol, xylitol, inositol And sugar alcohols such as maltitol, lactitol, palatinit, and erythritol.

The external preparation for skin may be one to which a thickener is added in order to adjust the viscosity. Examples of the thickener include water-soluble polymers. Examples of the water-soluble polymer used in the present invention include synthetic water-soluble polymers, semi-synthetic water-soluble polymers, and natural water-soluble polymers that can be generally used for external preparations.

Examples of the synthetic water-soluble polymer include acrylic acid methacrylic acid long chain alkyl (10 to 30 carbon atoms) copolymer, ethylene oxide-propylene oxide block copolymer, carboxyvinyl polymer, polyvinyl alcohol, polyvinyl pyrrolidone, polyvinyl methyl ether, Examples include sodium polyacrylate and polyethylene oxide. Among these, from the viewpoint of usability and the like, acrylic acid methacrylic acid long chain alkyl (C10-30) copolymer (for example, "PEMULEN TR-1" manufactured by BF Goodrich), carboxyvinyl, etc. Polymers (for example, “Carbopol 934”, “Carbopol 940”, “Carbopol 941” manufactured by BF Goodrich) are preferably used.

Examples of the semi-synthetic water-soluble polymer include carboxymethyl cellulose or a salt thereof (for example, sodium salt, potassium salt), cellulose-based polymer such as ethyl cellulose, methyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, methylhydroxypropyl cellulose; And starch-based water-soluble polymers such as carboxymethyl starch and methyl starch; alginic acid-based water-soluble polymers such as alginate propylene glycol ester and alginate; polysaccharide-based water-soluble derivatives.

Examples of natural water-soluble polymers include guar gum, locust bin gum, quince seed, carrageenan, galactan, gum arabic, tragacanth gum, pectin, mannan, starch, xanthan gum, dextran, succinoglucan, curdlan, hyaluronic acid, gelatin, casein, Examples include albumin and collagen. Among these, xanthan gum is preferably used from the viewpoint of usability and the like.

These water-soluble polymers may be used alone or in combination of two or more. Among these, a methacrylic acid long-chain alkyl (10 to 30 carbon atoms) copolymer, a carboxyvinyl polymer, and xanthan gum are preferable, and a methacrylic acid long-chain alkyl (10 to 30 carbon atoms) copolymer is particularly preferable. When the coalescence and xanthan gum are used in combination, the thickening effect is enhanced by the acrylic acid methacrylic acid long-chain alkyl (carbon number 10 to 30) copolymer, and the sticky feeling can be eliminated by the xanthan gum.

The method for producing the above-mentioned external preparation for skin is not particularly limited, and a conventional production method can be used. In any step of preparing the external preparation for skin, an appropriate method of adding gold ion water can be mentioned. it can.

The said gold ion can also be mix | blended with food-drinks. By containing the gold ion, a health food having superoxide anion scavenging ability can be obtained. Furthermore, food / beverage products with high safety | security can be obtained, preventing the corruption of food / beverage products.

It does not specifically limit as a form of the said food / beverage products, For example, a liquid, cream form, gel form, paste form, etc. can be mentioned. In addition, the type of food and drink of the present invention is not particularly limited, and examples thereof include confectionery (chewing gum, candy, gummi, tablet, chocolate, jelly, etc.), ice confectionery (ice candy, ice cream, sorbet, etc.), and frozen confectionery (jelly). , Pudding, water yokan, etc.), starch-based foods such as noodles, beverages (soup, coffee, teas, juice, carbonated beverages, cocoa, alcoholic beverages, jelly drinks, etc.), fat and oil foods (margarine, shortening, fat) Spreads), dairy products (milk, yogurt, whey drink, lactic acid bacteria drink, butter, cream, cheese, etc.).

The said food / beverage products can be suitably selected and mix | blended the following component in the range which does not impair the objective of this invention as needed. For example, sugar sweeteners (monosaccharides such as fructose, glucose, tagatose and arabinose, oligosaccharides such as lactose, oligosaccharides, maltose and trehalose, powdered starch syrup, dextrin, sugar alcohol etc.), high sweetness sweeteners (sucralose, Acesulfame K, stevia, etc.), polysaccharides such as starch, fats and oils, dairy products, stabilizers, emulsifiers, fragrances (vanillin, linalool, natural fragrances, etc.), pigments, colorants, acidulants, flavor ingredients (eggs, coffee, Tea, cocoa, fruit juice, yogurt, alcoholic beverages, etc.), flavoring (raspberry flavor, apple flavor, coffee flavor, etc.), anti-wetting agent, electrolyte, preservative, wetting agent, protein, amino acid, peptide, dietary fiber, Organic acids (citric acid, malic acid, fumaric acid, malonic acid, succinic acid, tartaric acid, lactic acid, etc.), vitamins (L-ascorbic acid, l-α-tocopherol, vitamin B1, vitamin B2, niacin, pantothenic acid, vitamin B6, vitamin B12, folic acid, biotin, inositol, etc., platinum, minerals (zinc, iron, calcium, magnesium, chromium, selenium, potassium, sodium) Etc.), marine collagen, hydrolyzed collagen, hyaluronic acid, fermented yeast extract, glucosamine, yeast, eggshell membranes, lycopene, astaxanthin, other carotenoids, silk, chondroitin, ceramide, placenta extract, shark fin extract, deep sea bream extract, squalene, γ-aminobutyric acid, casein decapeptide, raw chestnut skin extract, chestnut leaf extract, chestnut potato extract, chestnut flesh extract, chestnut bark extract, cabbage fermented extract, rose petal extract, grape leaves Extract, grape seed extract, apple Rephenol, chamomile extract, lychee seed extract, gotsukola extract, moon peach leaf extract, lotus germ extract, star fruit leaf extract, mulberry leaf extract, guava tea extract, red wine, green tea, black tea, oolong tea, coffee, cocoa, chocolate, Raw materials derived from eggs such as black sesame, beans, soy milk, nuts, mushrooms, green-yellow food vegetables, iodine eggs, catechins, other polyphenols, raspberry ketone, low molecular weight alginic acid, psyllium seed coat, ginkgo biloba extract, pine bark Extract, nattokinase, plant sterol, diacylglycerol, chitosan, hyaluronic acid, methylsulfonylmethane, kojic acid, ellagic acid, arbutin, lucinol, magnolignan, magnesium phosphate L-ascorbate, CoQ10, α-lipoic acid, etc. It is done.

In the above food and drink, the compounding amount of gold ions is preferably 0.0001 to 150 ppm, more preferably 0.001 to 100 ppm, and still more preferably 0.1 to 100 ppm.

It does not specifically limit as a manufacturing method of the said food / beverage products, The conventional manufacturing method can be used and the method of adding gold ion water suitably can be mentioned.

EXAMPLES The present invention will be further described below with reference to examples, but the present invention is not limited to these examples. In addition, all compounding quantities represent the mass%.

Superoxide anion scavenging ability and bactericidal performance were evaluated for gold ions obtained according to the production method described below.

(1) Manufacture of gold ion water After putting 1g of gold leaf (purity 99.99%) in a high heat resistant container (Chigel), 80ml of aqua regia (hydrochloric acid: nitric acid = 3: 1) is put in this high heat resistant container. The gold foil was dissolved in aqua regia. Next, this high temperature resistant container containing the gold solution was heated with a burner for 1 hour to evaporate and dry the gold solution.
After evaporating to dryness, the heat-resistant container was allowed to cool to room temperature, and then 80 ml of aqua regia was put into the heat-resistant container again, and the evaporated dry matter remaining in the heat-resistant container was dissolved in aqua regia. . The heat-resistant container containing the evaporated dry solid solution was heated with a burner for 1 hour to re-evaporate the evaporated dry solid solution.
After the heat-resistant container was radiated to room temperature again, the inside of the heat-resistant container was repeatedly washed with purified water, and all the washing liquid was transferred into a beaker while filtering to make the total volume 1 liter, thereby obtaining gold ion water. The gold ion concentration of the obtained gold ion water was measured with an ICP emission spectroscopic analyzer with n number = 5, and the average value of the results was 764 ppm.

(Bactericidal test)
The sample was inoculated with acne fungus solution (hereinafter referred to as “test solution”), stored anaerobically at room temperature, and the number of viable bacteria in the test solution was measured after 1, 3 and 24 hours. In addition, a preliminary test was performed in advance to examine a method for measuring the number of viable bacteria. The test was performed on samples with gold ion concentrations of 10 ppm, 5 ppm, and 2 ppm by diluting gold ion water obtained by the above-described method with ion exchange water.
Details of the test conditions are shown below.

1) Test strain
Propionibacterium acnes GAI 5419 (Acne bacteria)
2) Bacteria count culture medium and culture conditions GAM agar medium [Nissui Pharmaceutical Co., Ltd.] Pour plate culture method, 35 ° C ± 1 ° C, 5 days anaerobic culture 3) Preparation of test bacterial solution
The test bacterial solution was anaerobically cultured on a GAM agar medium at 35 ° C. ± 1 ° C. for 18-24 hours, then suspended in physiological saline and prepared so that the number of bacteria was 10 ⁸ to 10 ⁹ / ml. It was.
4) Test operation Sample stock solution and sample diluent (50 and 20 V / V% diluted solution of the sample prepared with purified water) stored in anaerobic condition overnight were used as samples. 10 ml of the sample was inoculated with 0.1 ml of the test bacterial solution to prepare a test solution. After anaerobic storage at room temperature, the test solution was immediately diluted 10 times with SCDLP medium [Nippon Pharmaceutical Co., Ltd.] after 1, 3 and 24 hours, and the number of viable bacteria in the test solution was measured using a medium for measuring the number of bacteria. .
In addition, it tested similarly using purified water as a control | contrast, and also measured viable count at the time of a start.

The results are shown in Table 1.
In addition, it was confirmed by a preliminary test that the number of viable bacteria can be measured without being influenced by the specimen by diluting the test solution 10 times with the SCDLP medium.

From the results in Table 1, it is clear that gold ions have a bactericidal performance for a long time at a low concentration. Moreover, since it has high sterilization performance even at a low concentration, it is excellent in terms of stability and safety.

(Measurement of superoxide anion scavenging action)
The test sample was added to Hank's buffer containing 0.25 mM nitroblue tetrazolium (NBT), 1 mM hypoxanthine and 0.1 mM EDTA. Next, xanthine oxidase (XOD) was added and incubated for 10 minutes, and then the absorbance of the reaction solution was measured (OD = 550 nm). On the other hand, a reaction liquid containing no XOD was prepared as an absorbance blank for the test sample, and similarly, the absorbance after incubation for 10 minutes was corrected by subtracting from the absorbance in the reaction system containing each reagent, thereby eliminating the superoxide anion of the test sample. Evaluated. The results are shown as relative values with the absorbance with no added sample taken as 100%. The measurement was performed for various concentrations shown in Table 1. Further, as a control test, the same test was performed for ascorbic acid and theaflavin, which are known as compounds having a superoxide anion scavenging ability. The results are shown in Table 2. Further, the concentration was plotted on the horizontal axis and the absorbance was plotted on the vertical axis, and the value at which the absorbance was 50 was read from the graph as ID50. The values are shown in Table 3. The measurement was performed at n = 5, and the average value and standard deviation were calculated.

From the results of Tables 2 and 3, it became clear that gold ions exhibit a high level of superoxide anion scavenging action even at low concentrations.

The disinfectant of the present invention can be blended for the purpose of preventing the decay of compositions such as cosmetics and foods. Furthermore, it can also mix | blend as an active ingredient in the skin external preparation for the purpose of the treatment and prevention of acne. Furthermore, it can also mix | blend as an active ingredient in the composition for disinfection and disinfection. Furthermore, it can also mix | blend as an active ingredient in the composition for household sterilization.

Claims (2)

A disinfectant used for disinfecting acne bacteria containing gold ions as an active ingredient. A therapeutic / preventive agent for acne, which is an aqueous composition characterized by containing gold ions.