JP5580505B2 - 遺伝子構築細胞モニターシステム - Google Patents

遺伝子構築細胞モニターシステム Download PDF

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JP5580505B2
JP5580505B2 JP2003525297A JP2003525297A JP5580505B2 JP 5580505 B2 JP5580505 B2 JP 5580505B2 JP 2003525297 A JP2003525297 A JP 2003525297A JP 2003525297 A JP2003525297 A JP 2003525297A JP 5580505 B2 JP5580505 B2 JP 5580505B2
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protein
cell
enzyme
fusion protein
cells
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JP2005501548A5 (https=
JP2005501548A (ja
Inventor
ツァオ シャローン
ヴァインシュテイン イナ
エグレン リチャード
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ディスカヴァーエックス コーポレイション
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    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00Methods of screening libraries
    • C40B30/04Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62DNA sequences coding for fusion proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845Methods of identifying protein-protein interactions in protein mixtures
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/60Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/80Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90Enzymes; Proenzymes
    • G01N2333/914Hydrolases (3)
    • G01N2333/924Hydrolases (3) acting on glycosyl compounds (3.2)
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00Screening for compounds of potential therapeutic value
    • G01N2500/20Screening for compounds of potential therapeutic value cell-free systems
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00Detection or diagnosis of diseases
    • G01N2800/52Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2003525297A 2001-08-30 2002-08-27 遺伝子構築細胞モニターシステム Expired - Lifetime JP5580505B2 (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US31642801P 2001-08-30 2001-08-30
US60/316,428 2001-08-30
US34315601P 2001-10-21 2001-10-21
US60/343,156 2001-10-22
US35308602P 2002-01-30 2002-01-30
US60/353,086 2002-01-30
PCT/US2002/027497 WO2003021265A1 (en) 2001-08-30 2002-08-27 Genetic construct intracellular monitoring system

Publications (3)

Publication Number Publication Date
JP2005501548A JP2005501548A (ja) 2005-01-20
JP2005501548A5 JP2005501548A5 (https=) 2008-11-20
JP5580505B2 true JP5580505B2 (ja) 2014-08-27

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ID=27405870

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2003525297A Expired - Lifetime JP5580505B2 (ja) 2001-08-30 2002-08-27 遺伝子構築細胞モニターシステム

Country Status (6)

Country Link
US (1) US7479377B2 (https=)
EP (1) EP1421385B1 (https=)
JP (1) JP5580505B2 (https=)
AU (1) AU2002332724A1 (https=)
CA (1) CA2458879C (https=)
WO (1) WO2003021265A1 (https=)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1582332A (zh) * 2001-12-29 2005-02-16 Seoul大学校产学协力财团 转染gfp的克隆猪、敲除gt的克隆猪及其生产方法
EP1556682B8 (en) * 2002-10-21 2010-02-17 DiscoveRx Corporation Ip3 protein binding assay
JP2007521021A (ja) * 2003-11-06 2007-08-02 ディスカヴァーエックス インコーポレイテッド 細胞膜タンパク質の検定
US8586294B2 (en) * 2004-05-18 2013-11-19 The Board Of Trustees Of The Leland Stanford Junior University Detection of protein translocation by beta-galactosidase reporter fragment complementation
JP4796059B2 (ja) 2004-06-30 2011-10-19 ディスカヴァーエックス コーポレイション 細胞内修飾の分析
WO2007002200A2 (en) * 2005-06-21 2007-01-04 Discoverx, Inc. Mitotic index assay
WO2009043953A1 (es) * 2007-10-05 2009-04-09 Proyecto De Biomedicina Cima, S.L. Empleo de inhibidores de ppar alfa truncado en el tratamiento de la insuficiencia cardiaca en sujetos con cardiopatía hipertensiva
US8865421B2 (en) * 2008-07-02 2014-10-21 Discoverx Corporation Assays for nuclear hormone receptor binding
US20100120063A1 (en) * 2008-10-10 2010-05-13 Discoverx Corporation GPCR Arrestin Assays
US8211655B2 (en) * 2009-02-12 2012-07-03 Discoverx Corporation Wild-type receptor assays
GB0905419D0 (en) 2009-03-30 2009-05-13 Ge Healthcare Uk Ltd Methods for testing ligand binding to G protein-coupled receptors
GB0917877D0 (en) 2009-10-13 2009-11-25 Ge Healthcare Uk Ltd Enzyme fragment complementation assays for monitoring the activiation of the voltage-gated potassium ion channel herg
WO2013119579A1 (en) * 2012-02-06 2013-08-15 Discoveryx Corporation Detection of intracellular binding events by measuring protein abundance

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5604091A (en) * 1984-03-01 1997-02-18 Microgenics Corporation Methods for protein binding enzyme complementation
US4708929A (en) * 1984-10-29 1987-11-24 Microgenics Corporation Methods for protein binding enzyme complementation assays
DE69731960T2 (de) * 1996-09-24 2005-12-15 Cadus Pharmaceutical Corp. Verfahren und zusammensetzungen für die identifizierung von receptor effectoren
US6037133A (en) * 1998-04-17 2000-03-14 Clontech Laboratories, Inc. IκBEGFP constructs, cell lines and methods of use

Also Published As

Publication number Publication date
CA2458879C (en) 2015-04-28
EP1421385A4 (en) 2004-12-01
WO2003021265A1 (en) 2003-03-13
WO2003021265A8 (en) 2004-07-01
AU2002332724A1 (en) 2003-03-18
US7479377B2 (en) 2009-01-20
JP2005501548A (ja) 2005-01-20
EP1421385B1 (en) 2016-06-29
CA2458879A1 (en) 2003-03-13
US20030092070A1 (en) 2003-05-15
EP1421385A1 (en) 2004-05-26

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