JP5494986B2 - 澱粉結合ドメインを含む組換えタンパク質とその使用 - Google Patents
澱粉結合ドメインを含む組換えタンパク質とその使用 Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
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- C07—ORGANIC CHEMISTRY
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- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
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- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
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Description
表I.不溶性澱粉に関して脱着等温線により算出されたRoGACBM21誘導体の親和性
表II.蛍光滴定のスペクトルにより算出された可溶性少糖類に対するRoGACBM21誘導体の親和性
amYAspka/538-634 TLPITFEELV TTTYGEEVYL SGSISQLGEW HTSDAVKLSA DDYTSSNPEW SVTVSLPVGT TFEYKFIKVD EGGSVTWESD PNREYTVPEC GSGSGET
amYAspnd/521-617 AITVVFQERV QTAYGENVFL AGSISQLGNW DTTEAVALSA AQYTATDPLW TVAIELPVGT SFEFKFLKKR QDGSIVWESN PNRSAKVNEG CARTTQT
amYBacsp/515-608 TSNVTFTVNN ATTTSGQNVY VVANIPELGN WNTANAIKMN PSSYPTWKAT IALPQGKAIE FKFIKKDQAG NVIWESTSNR TYTVPFSSTG SYTA
amYCrYsp/528-624 AGTVVFDVYV QTQYGQSVVI AGNIPQLGNW SPANGLNLNA NQYTASSPKW TGTITGVAPG TTFQWKPIVV TNGNDNWYPG NNQQATTGSA CSSPAAD
amYStrgr/470-562 QTSASFHVNA TTAWGENIYV TGDQAALGNW DPARALKLDP AAYPVWKLDV PLAAGTPFQY KYLRKDAAGK AVWESGANRT ATVGTTGALT LND
amYStrlm/470-562 QTSASFHVNA TTAWGENIYV TGDQAALGNW DPARALKLDP AAYPVWKLDV PLAAGTPFQY KYLRKDAAGK AVWESGANRT ATVGTTGALT LND
amYStrlil/480-570 RRVPSAVDAT TSWGQNIYVT GNRPELGNWN PGGALQLDPA AYPVWKRDVE LPEGTTFEYK YLRKDDAGNV TWESGANRTA TVNTTKTTLN D
amYStrli2/478-569 SSGVSFAVDA TTSWGQDIYV TGNRPGLGHW DPGGGLQLDP AAYPVWKRDV ELPEGTTFEY KSLRKADAGN VTWESGANRT ATVNTTKTTL ND
amYStrvi/473-565 TSGASFNVTA TTVVGQNIYV TGNRAELGNW APASALKLDP ATYPVWKLTV GLPAGTSFEY KYIRKDAAGN VTWESGANRT ATVPASGQLV LND
amYThncu/505-597 TVTARFHATV TTWYGQEVAV VGSIPELGSW QPAQGVRLRT DSGTYPVWSG AVDLPAGVGF EYKYVKLNRT APWSGSRAAT ASPPWMTSGG GCS
amY AspaW/534-630 TLPITLEELV TTTYGEEIYL SGSISQLGEW DTSDAVKLSA DDYTSSNPEW YVTVSLPVGT TFEYKFIKVE EDGSVTWESD PNREYTVPEC GSGETVV
cgtBacag/580-677 QVTVRFIIDN AETKLGENVF LVGNVHELGN WDPEQSVGRF FNQIVYQYPT WYYDVNVPAN TDLEFKFIKI DQDNNVTWQS GANQTYSSPE SGTGIIRV
cgtBacbr/592-692 QVSVRFAVNN ATTNSGTNVY IVGNVSELGN WDPNKAIGPM FNQVMYKYPT WYYDISVPAG KNLEYKYIKK DHNGNVTWQS GNNRTYTSPA TGTDTVISNW
cgtBacci2/613-709 QVSVRFVVNN ATTALGQNLY LTGSVSELGN WDPAKAIGPM YNQVVYQYPN WYYDVSVPAG KTIEFKFLKK QGSTVTWEGG SNHTFTAPSS GTATINV
cgtBacci8/613-709 QVSVRFVVNN ATTALGQNVY LTGNVSELGN WDPAKAIGPM YNQVVYQYPN WYYDVSVPAG KTIEFKFLKK QGSTVTWEGG SNHTFTAPSS GTATINV
cgtBacciA/613-709 QVSVRFVVNN ATTALGQNVY LAGSVSELGN WDPAKAIGPL YNQVIYQYPT WYYDVTVPAG KTIEFKFLKK QGSTVTWEGG SNHTFTAPTS GTATINV
cgtBaccl/601-698 QESVRFVVDN AHTNYGENVY LVGNVPELGN WNPADAIGPM FNQVVYSYPT WYYDVSVPAD TALEFKFIIV DGNGNVTWES GGNHNYRVTS GSTDTVRV
cgtBacli/618-715 QVSVRFVINN ATTALGENIY LTGNVSELGN WTTGAASIGP AFNQVIHAYP TWYYDVSVPA GKQLEFKFFK KNGATITWEG GSNHTFTTPT SGTATVTI
cgtBacmal/614-710 QVTVRFLVNQ ANTNYGTNVY LVGNAAELGT WDPNKAIGPM YNQVIAKYPS WYYDVSVPAG TKLDFKFIKK GGGTVTWEGG GNHTYTTPAS GVGTVTVDWQ N
cgtBacma2/613-713 QVTVRFKVNN ATTALGQNVY LTGNVAELGN WTAANAIGPM YNQVEASYPT WYFDVSVPAN TALQFIFIKV NGSTVTWEGG NNHTFTSPSS GVATVTVDWQ N
cgtBacoh/604-701 QVSIRFAVNN ATTSLGTNLY MVGNVNELGN WDPDQAIGPM FNQVMYQYPT WYYDISVPAE ENLEYKFIKK DSSGNVVWES GNNHTYTTPA TGTDTVLV
cgtBacspO/613-709 QVTVRFVINN ATTALGQNVF LTGNVSELGN WDPNNAIGPM YNQVVYQYPT WYYDVSVPAG QTIEFKFLKK QGSTVTWEGG ANRTFTTPTS GTATVNV
cgtBacspl/604-701 QVSVRFGVNN ATTSPGTNLY IVGNVNELGN WDADKAIGPM FNQVMYQYPT WYYDISVPAG KNLEYKYIKK DQNGNVVWQS GNNRTYTSPT TGTDTVMI
cgtBacsp7/613-709 QVSVRFVVNN ATTALGQNVY LAGSVSELGN WDPAKAIGPL YNQVIYQYPT WYYDVTVPAG KTIEFKFLKK QGSTVTWEGG SNHTFTAPTS GTATINV
cgtBacsp3/612-708 QVTVRFVINN ATTALGQNVF LTGNVSELGN WDPNNAIGPM YNQVVYQYPT WYYDVSVPAG QTIEFKFLKK QGSTVTWEGG ANRTFTTPTS GTATVNV
cgtBacsp63/618-715 QVTVRFVINN ASTTLGQNIY LTGNVAELGN WSTGSTAIGP AFNQVIHQYP TWYYDVSVPA GKELEFKFFK KNGSTITWEG GSNHKFTTPA SGTATVTV
cgtBacsp6/604-701 QVSIRFAVNN ATTSLGTNLY IVGNVNELGN WDPDQAIGPM FNQVMYQYPT WYYDISVPAE ENLEYKFIKK DSSGNVVWES GNNHTYTTPA TGTDTVLV
cgtBacspB/613-709 QVSVRFVVNN ATTALGQNLY LTGNVSELGN WDPAKAIGPM YNQVVYQYPN WYYDVSVPAG KTIEFKFLKK QGSTVTWEGG SNHTFTAPSS GTATINV
cgtBacspD/598-699 QTSVRFVVNQ AETSVGENLY VVGDVPELGS WDPDKAIGPM FNQVLYSYPT WYYDVSVPAN QDIEYKYIMK DQNGNVSWES GNNHIYRTPE NSTGIVEVNF NQ
cgtBacspK/626-723 QVSVRFGVNN ATTSPGTNLY IVGNVNELGN WDADKAIGPM FNQVMYQYPT WYYDISVPAG KNLEYKYIKK DKQNGNVVWQS GNNRTYTSPT TGTDTVMI
cgtBacst/610-711 QVSVRFVVNN ATTNLGQNIY IVGNVYELGN WDTSKAIGPM FNQVVYSYPT WYIDVSVPEG KTIEFKFIKK DSQGNVTWES GSNHVYTTPT NTTGKIIVDW QN
cgtGeost/610-707 QVSVRFVVNN ANTNWGENIY LVGNVHELGN WNTSKAIGPL FNQVIYSYPT WYVDVSVPEG KTIEFKFIKK DGSGNVIWES GSNHVYTTPT STTGTVNV
cgtKlepn/559-654 VQSINFTCNN GYTISGQSVY IIGNIPQLGG WDLTKAVKIS PTQYPQWSAS LELPSDLNVE WKCVKRNETN PTANVEWQSG ANNQFNSNDT QTTNGS
cgtThetu/610-706 QICVRFVVNN ASTVYGENVY LTGNVAELGN WDTSKAIGPM FNQVVYQYPT WYYDVSVPAG TTIQFKFIKK NGNTITWEGG SNHTYTVPSS STGTVIV
cgtThcsp/634-735 QIPAIFEVRN TQGTNLETQV GEFLWLTGSV PELSYWSPET IKAVGPMLCP GWPDWFVVAS VPADTYIEFK FLKAPLGGTG IWEVGSNHAY LTPSSGIGEV SV
cgt_Bacsp5/612-712 QVTVRFVINN ATTALGQNVF LTGNVSELGN WDPNNAIGPM YNQVVYQYPT WYYDVSVPAG QTIEFKFLKK QGSTVTWEGG ANRTFTTPTS GTATVNVNWQ P
cgt_Glovi/542-637 REIVRIQVNG FRTQPGEVVA VIGDCPELGD WDLSRAFRLE YINDNTWFGE IPFNKSANQI VAYKYVIFRE NGPPINENRT SRRRFVPDKS IAKWRD
cgt_Nossp7/539-634 QTIVRVQLNG VHTQPGETIV VVGDCPELGN WDISKAYPLE YINSNTWFAE IPFDESAGKL ISYKYAMWRE GRSPLRENIL NRRWVVAKEG TVKWRD
cgt_Nossp9/539-634 QTIVRAQLNG VQTQPGETIV VIGDCPELGN WDISKAYPLE YINTNWFAE IPFNESAGKL IAYKYALWRE GQSPLRENLV NRRWVIAKEG TVKWRD
cgt_StcpY/611-710 QIPVRLLIND FKTVPGEQLY LMGDVFEMGA NDAKNAVGPL FNNTQTIAKY PNWFFDTHLP INKEIAVKLV KKDSIGNVLW TSPETYSIKT GHEAQTITIK
m5hPsespK/514-609 VLSLTFNETA DTVWGQNLFV VGNVGALGNW APAAGAAMTW ISGSGSTGQW RATVQLPADT PVQYKYVKKD GAGNVVWESG GNRVVTTPAP GATIAV
m4hPsesa/454-550 LVNVNFRCDN GVTQMGDSVY AVGNVSQLGN WSPASAVRLT DTSSYPTWKG SIALPDGQNV EWKCLIRNEA DATLVRQWQS GGNNQVQAAA GASTSGS
m4hPsest/451-547 LVSVSFRCDN GATQMGDSVY AVGNVSQLGN WSPAAALRLT DTSGYPTWKG SIALPAGQNE EWKCLIRNEA NATQVRQWQG GANNSLTPSE GATTVGR
maaBacst/614-715 QTSVVFTVKS APPTNlGDKI YLTGNIPELG NWSTDTSGAV NNAQGPLLAP NYPDWFYVFS VPAGKTIQFK FFIKRADGTI QWENGSNHVA TTPTGATGNI TV
apuBacst/1339-1458 MVQVTFKVRA PSYTPLDTRI TIPNSLNGWN TGAWEMTRGG AVTSDWEFTT ELQEGETI IY KYVKGASWDQ EGLADHTRDD QTDDDVSYYG YGAIGTDLKI TVQNQGNNKM IIQDYILRWI
apuBacspX/1335-1454 PDIVMVQVTF KVKAPSYTPL DTRITIPNSI NGWNTGAWEM TRGGAVTPDW EFTTELQEGE TITYKYVKGG SWDQEGLADH TRDDQTDDDV SYYGYGAIGT ELKVTVHNQG NNKMVIQDYI LRWI
apuTheth/1251-1353 PIKVIFNVTV PDYTPDAVNL AGTFPNATWD PSAQQMTKID NNTYSITLTL DEGTQIEYKY ARGSWDKVEK DEYGNEFASN RKVTIVNQGN NEMTINDTVY RWR
apuTheet/1254-1356 PIKVIFNVTV PDYTPDDGAN IAGNFHDAFW NPSAHQMTKT GPNTYSITLT LNEGTQLEYK YARGSWDKVE KGEYGEEIAN RKITVVNQGS NTMVVNDTVQ RWR
apuThetc/1254-1361 PIKVTFNVTI PDYTPDDGVN IAGNFPDAFW NPNANQMTKA GSNTYSITLT LNEGTQIEYK YARGSWDKVE KGEYGNEIDN RKITVVNQGS NTMVVNDTVQ RWR
bmYBacce/452-545 QTIVVKNVPT TIGDTVYITG NRAELGSWDT KQYPIQLYYD SHSNDWRGNV VLPAERNIEF KAFIKSKDGT VKSWQTIQQS WNPVPLKTTS HTSS
bmYBacme/452-544 QTVVVKNAPT ALGETVYIVG DRAELGQWDT SIYPIKLTYN SSTADWRGTV HFPASQNVQF KAIVKRADGS LKAWQPSQQY WSVPSTTTTY TDN
bmYCloth/453-547 TIPVTFTINN ATTYYGQNVY IVGSTSDLGN WNTTYARGPA SCPNYPTWTI TLNLLPGEQI QFKAVKIDSS GNVTWEGGSN HTYTVPTSGT GSVTI
gmYAspaW/537-633 AVAVTFDLTA TTTYGENIYL VGSISQLGDW ETSDGIALSA DKYTSSNPLW YVTVTLPAGE SFEYKFIRVE SDDSVEWESD PNREYTVPQA CGESTAT
gmYAspFi/538-634 AVAVTFDLTA TTTYGENIYL VGSISQLGDW ETSDGIALSA DKYTSSDPLW YVTVTLPAGE SFEYKFIRIE SDDSVEWESD PNREYTVPQA CGTSTAT
gmYAspka/537-633 AVAVTFDLTA TTTYGENIYL VGSISQLGDW ETSDGIALSA DKYTSSNPLW YVTVTLPAGE SFEYKFIRVE SDDSVEWESD PNREYTVPQA CGESTAT
gmYAspni/538-634 AVAVTFDLTA TTTYGENIYL VGSISQLGDW ETSDGIALSA DKYTSGDPLW YVTVTLPAGE SFEYKFIRIE SDDSVESWESD PNREYTVPQA CGTSTAT
gmYAspor/511-606 TVSVTFAVKA TTVYGESIKI VGSISQLGSW NPSSATALNA DSYTTDNPLW TGTINLPAGQ SFEYKFIRVQ NGAVTWESDP NRKYTVPSTC GVKSAV
gmYAspsh/537-633 AVAVTFDLTA TTTYGENIYL VGSISQLGDW ETSDGIALSA DKYTSSNPPW YVTVTLPAGE SFEYKFIRVE SDDSVEWESD PNREYTVPQA CGESTAT
gmYCorro/482-579 SVEVTFDVYA TTVYGQNIYI TGDVSELGNW TPANGVALSS ANYPTWSATI ALPADTTIQY KYVNIDGSTV IWEDAISNRE ITTPASGTYT EKDTWDES
gmYHorre/506-602 QVSITFNINA TTYYGENLYV IGNSSDLGAW NIADAYPLSA SAYTQDRPLW SAAIPLNAGE VISYQYVRQE DCDQPYIYET VNRTLTVPAC GGAAVTT
gmYHumgr/514-611 EVYVTFNERV STAWGETIKV VGNVPALGNW DTSKAVTLSA SGYSNDPLW SITVPIKATG SAVQYKYIKV GTNGKITWES DPNRSITLQT ASSAGKCA
gmYLened/476-568 AVSVTFNVDA STLEGQNVYL TGAVDALEDW STDNAILLSS ANYPTWSVTV DLPGSTDVQY KYIKKDGSGT VTWESDPNME ITIPANGTYA TND
gmYNeucr/525-621 EVLVTFNEKV TTSYGQTVKV VGSIAALGNW APASGVTLSA KQYSSSNPLW STTIALPQGT SFKYKYVVVN SDGSVKWEND PDRSYAVGTD CASTATL
gmYTalem/516-612 SVAVTFDEIV STSYGETIYL AGSIPELGNW STASAIPLRA DAYTNSNPLW YVTVNLPPGT SFEYKFFKNQ TDGTIVWEDD PNRSYTVPAY CGQTTAI
gmY AspaW/537-633 AVAVTFDLTA TTTYGENIYL VGSISQLGDW DTSDGIALSA DKYTSSNPLW YVTVTLPAGE SFEYKFIRIE SDDSVEWESD PNREYTVPQA CGESTAT
gmY_AspniT/537-633 AVAVTFDLTA TTTYGENIYL VGSISQLGDW ETSDGIALSA DKYTSSDPLW YVTVTLPAGE SFEYKFIRIE SDDSVEWESD PNREYTVPQA CGTSTAT
gmY Neucr/363-460 TVAVTFNHLA STSYGESIKI VGSISQLGSW SASSGVALSA SQYTTSNPLW TATVSLPAGT KFEYKFVKVS SEGSAVTWES DPNRSYTVPQ SCAESVAV
6agtArtgl/864-962 SVWATFSCEN ATTTFGQSVY VVGNVPQLGN WSPADAVKLE PSAYPTWTGV VRNLPPSSTV EWKCIKRQEA GLPNTADAWE PGGNNILSTP PSGSAGITT
4agtBacFr/149-212 LAICGNQKAL GNWDPDKAWP MSDANFPEWQ AELDASKLEF PLEYKFVLYN KEEKRAEAWE NNPN
4agtSoltu/8-104 SRKVSFRIPY YTQWGQNLLI CGSDRLLGSW NVKKGLLLKP SHQGEVLVWS GSIPVPPGYQ SEYSYYVVDD RRNILRWEVG KKRKLLLPDG LQDGQSL
4agt OrYsa/12-108 TVTLVFKLPY YTQWGQSLLI AGSEPALGSW NVKQGLSLSP VHQGNELIWS GRVSVATGFT CQYMNYYVVDD NKNVLRSESG EKRKLVLPEG VQDGDVV
agWdArath/73-155 KVRLNVRLDH QVNFGDHVAM FGSAKEIGSW KKKSPLNWSE NGWVCELELD GGQVLECKFV IVKNDGSLSW ESGDNRVLKV PNSGNFSVVC HWD
genHomsa/263-354 QVSVRFQVHY VTSTDVQFIA VTGDHECLGR WNTYIPLHYN KDGFWSHSIF LPADTVVEWK FVLVENGGVT RWEECSNRFL ETGHEDKVVH AW
laFGalga/2-117 LFRFGVVLPA RIAEGGGALL VAGSRPELGE WDPQRAVPMQ PARPAAALAA QEPVLWLGEV LLSDEDTASP FWYKFLRREG GQLLWEGNGP HHDRSCVYNQ SNIVDGVYCL PIAHWI
laFHomsa/2-130 RFRFGVVVPP AVAGARPELL VVGSRPELGR WEPRGAVRLR PAGTAAGDGA LALQEPGLWL GEVELAAEEA AQDGAEPGRV DTFWYKFLKR EPGGELSWEG NGPHHDRCCT YNENNLVDGV YCLPIGHWI
depChlpr/21-110 KAKVQFRLPK RVSFGQTISI VTSRSGWEPI PDLHMDWSEG DEWKVSAEVA PGDELEYKYV VLGPSGIVEW QTGSNRRLAV DVPGGAAVTV
amyLipko/40-133 ESVTGSNHVQ LASYEMCGST LSASLYVYND DYDKIVTLYY LTSSGTTGST LALILPVWSN NWELWTLSAI AAGAVEITGA SYVDSDTSVT YTTS
amyLipst/40-133 ESVTSSNHVQ LASHEMCDST LSASLYIYND DYDKIVTLYY LTSSGTTGSV TASYSSSLSN NWELWSLSAP AADAVEITGA SYVDSDASAT YATS
gmyArxad/26-132 NSPPDDKAVA LSSYSYCGGY LSASAFVKNL SYDKLVTLYW TNADNKSTPL NAGSLDYVKA ASDDQSWELW SLNVTTVPDG VDALLNITYV AASIGKTNSQ QLNVQVE
gmyRhior/26-130 ASIPSSASVQ LDSYNYDGST FSGKIYVKNI AYSKKVTVIY ADGSDNWNNN GNTIAASYSA PISGSNYEYW TFSASINGIK EFYIKYEVSG KTYYDNNNSA NYQVS
gmyMucci/25-129 ETVPTTEAVK VKSFTYDGST LAGQIYIKNI AYTKTVTVIY SDASDNWNNN GNTIAASYSA AIAGTNYEYW TFSAPVSGIK QFYVKYVVSG TTYYDNNNSG NYQVS
pfHomsa/122-230 QLQIQKAILE STESLLGSTS IKGIIRVLNV SFEKLVYVRM SLDDWQTHYD ILAEYVPNSC DGETDQFSFK IVLVPPYQKD GSKVEFCIRY ETSVGTFWSN NNGTNYTFI
Claims (11)
- 野生型の澱粉結合ドメインの炭水化物結合活性と比較して低下した炭水化物結合活性を有する改変された澱粉結合ドメインであって、SEQ ID No.2または3のアミノ酸シーケンスの32番目、47番目、67番目、83番目、または93番目のアミノ酸残基に変異を有するアミノ酸シーケンスを有することを特徴とする改変された澱粉結合ドメイン。
SEQ ID No.2:ASIPSSASVQ LDSYNYDGST FSGKIYVKNI AYSKKVTVIY ADGSDNWNNN GNTIAASYSA PISGSNYEYW TFSASINGIK EFYIKYEVSG KTYYDNNNSA NYQVSTS
SEQ ID No.3:ASIPSSASVQ LDSYNYDGST FSGKIYVKNI AYSKKVTVIY ANGSDNWNNN GNTIAASYSA PISGSNYEYW TFSASINGIK EFYIKYEVSG KTYYDNNNSA NYQVSTS - 前記32番目、47番目、67番目、83番目、または93番目のアミノ酸残基の変異は、アラニンへの置換であることを特徴とする、請求項1に記載の改変された澱粉結合ドメイン。
- 請求項1に記載の改変された澱粉結合ドメインおよび標的ポリペプチドを含む組換えタンパク質。
- 請求項1に記載の改変された澱粉結合ドメインをコードする遺伝子を含む発現ベクター。
- 式
(X−X)n
(式中、Xはグルコース分子であり、グルコースとグルコースとの間の連鎖がα−1,4−連鎖またはα−1,6−連鎖であり、nが1以上であり、その構造の一部に、主鎖、側鎖、または修飾残基を含む)で示される化合物を含有する親和性マトリックスと結合するための、請求項1に記載の改変された澱粉結合ドメインの使用。 - 前記親和性マトリックスが、前記改変された澱粉結合ドメインの32番目、47番目、67番目、83番目、または93番目のアミノ酸残基に結合することを特徴とする、請求項5に記載の使用。
- (a)請求項3に記載の組換えタンパク質を含有する生物学的液体を親和性マトリックスに直接適用することと、
(b)溶出緩衝液により前記組換えタンパク質を溶出することとを含み、前記親和性マトリックスが、
式
(X−X)n
(式中、Xはグルコース分子であり、グルコースとグルコースとの間の連鎖が、α−1,4−連鎖またはα−1,6−連鎖であり、nは1以上であり、その構造の一部に、主鎖、側鎖、または修飾残基を含む)で示される化合物を含有することを特徴とする、組換えタンパク質を精製するための方法。 - 前記親和性マトリックスが、前記改変された澱粉結合ドメインの32番目、47番目、67番目、83番目、または93番目のアミノ酸残基に結合することを特徴とする、請求項7に記載の方法。
- 請求項4に記載の発現ベクターを含むことを特徴とする、組換えタンパク質を精製するためのキット。
- 前記改変された澱粉結合ドメインに結合する親和性マトリックスをさらに含むことを特徴とする、請求項9に記載のキット。
- 前記組換えタンパク質および前記親和性マトリックスを分離するために使用される溶出緩衝液をさらに含むことを特徴とする、請求項9に記載のキット。
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US20100048880A1 (en) * | 2006-10-31 | 2010-02-25 | Simpson Biotech Co., Ltd. | Starch Binding Domain and Use Thereof |
US8551695B2 (en) * | 2006-10-31 | 2013-10-08 | Simpson Biotech Co., Ltd. | Starch binding domain and use thereof |
EP2641589A1 (en) | 2008-04-11 | 2013-09-25 | Danisco US Inc. | Alpha-glucanase and oral care composition containing the same |
US20100291540A1 (en) * | 2009-05-12 | 2010-11-18 | National Tsing Hua University | Carbohydrate binding module and use thereof |
US20100291656A1 (en) * | 2009-05-15 | 2010-11-18 | Simpson Biotech Co., Ltd. | Method and system for protein purification |
US8986955B2 (en) * | 2009-05-15 | 2015-03-24 | Simpson Biotech Co., Ltd. | Method for increasing thermal stability and retaining activity of a protein |
WO2011059828A1 (en) * | 2009-10-29 | 2011-05-19 | University Of Kansas | Methods of producing and purifying proteins |
WO2013021060A1 (en) | 2011-08-10 | 2013-02-14 | Novozymes A/S | Polypeptides having peroxygenase activity and polynucleotides encoding same |
CA2957820A1 (en) * | 2014-08-22 | 2016-02-25 | Nectagen, Inc. | Affinity proteins and uses thereof |
CN111551716B (zh) * | 2020-06-10 | 2023-02-28 | 安徽大学 | 一种α-淀粉酶的淀粉结合结构域与非淀粉类底物的结合判断方法 |
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US5643758A (en) | 1987-03-10 | 1997-07-01 | New England Biolabs, Inc. | Production and purification of a protein fused to a binding protein |
US5137819A (en) | 1988-07-08 | 1992-08-11 | University Of British Columbia | Cellulose binding fusion proteins for immobilization and purification of polypeptides |
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