JP5455333B2 - Aging odor removal composition - Google Patents

Description

  The present invention relates to a composition that suppresses active oxygen involved in generation of 2-nonenal, which is a main causative substance of an aging odor, or changes 2-nonenal to odorless.

Under the recent deodorant boom, body odor generated with aging has attracted attention, and the technology to remove it by adsorbing it on a porous carrier such as charcoal or coconut husk activated carbon (Patent Document 1), the propagation of causative resident bacteria A technique for suppressing the above (Patent Document 2) and a technique for masking with other fragrance components have been developed.
Thus, the body odor specifically generated with aging is called an aging odor, which is considered unpleasant with scalp odor, and its elimination is required.

On the other hand, 2-nonenal has been proposed as a main causative substance of an aging odor, and this component has been shown to increase specifically after the age of 40, which is the age at which an aging odor occurs. 2-nonenal is generated by oxidative degradation of ω7 unsaturated fatty acid, and it has been shown that lipid peroxide promotes oxidative degradation (Non-patent Document 1).
However, with the conventional technology, it has been difficult to suppress the generation of 2-nonenal and to convert 2-nonenal once generated to odorless.
JP 2002-145747 A JP2008-110999 Haze et al., The Journal of Investigative Dermatology, Vol. 116, No.4 (2001), pp.520-524.

  Under these circumstances, the generation of 2-nonenal, which is the main causative substance of aging odor, is effectively suppressed, and the generated 2-nonenal is converted into odorless, thereby fundamentally reducing the aging odor. There is a desire to solve this problem.

  Therefore, as a result of intensive studies on a substance that suppresses the generation of 2-nonenal and converts the generated 2-nonenal into an odorless substance, the present inventor has generated active oxygen that causes glucose to generate 2-nonenal. In addition, the present inventors have found that cysteine chemically reacts with 2-nonenal to be converted into odorless substances, and thus completed the present invention.

That is, the present invention
(1) A composition for suppressing the generation of body odor, comprising water and glucose;
(2) Water and alanine, arginine, asparagine, aspartic acid, cysteine, N-acetylcysteine, cystine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine A composition for removing body odor, comprising one or more amino acids selected from valine;
(3) Water, alanine, arginine, asparagine, aspartic acid, cysteine, N-acetylcysteine, cystine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine A composition for suppressing and removing body odor, comprising one or more amino acids selected from valine and glucose;
(4) The composition according to (1) or (3) above, which contains 7.5 to 150 mM of glucose;
(5) Alanine, arginine, asparagine, aspartic acid, cysteine, N-acetylcysteine, cystine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, valine The composition according to (2) or (3) above, which contains 0.1 to 50 mM of one or more amino acids selected from
(6) The composition according to any one of (1) to (5), which is a skin cosmetic additive;
(7) The composition according to (6), which is a deodorant additive;
(8) A skin cosmetic comprising the skin cosmetic additive according to (6) above;
(9) The present invention provides a deodorant characterized by containing the deodorant additive as described in (7) above.

According to the present invention, the problems of unpleasant aging odor and scalp odor can be fundamentally solved.
Moreover, the component mix | blended with the composition of this invention is mix | blended with various skin cosmetics etc. conventionally, and the safety | security is confirmed.

In the first aspect, the present invention provides a composition for suppressing body odor generation.
The “body odor” used in the present specification mainly refers to the above-mentioned aging odor and scalp. In addition to the body odor generally referred to as aging odor, 2-nonenal is the main causative substance. Includes other body odors.

  As the glucose used in the body odor generation suppressing composition of the present invention, D-glucopyranose which is naturally present in a large amount can be used. Glucose has traditionally been used as one of the three major nutrients, and its application to the skin is formulated into the composition as an aqueous solution in order to further diffuse and penetrate it.

  The concentration of glucose in the composition for suppressing body odor generation of the present invention is usually 7.5 to 150 mM, preferably 10 to 100 mM, more preferably 15 to 50 mM, and most preferably 20 to 30 mM. When the concentration of glucose in the composition for suppressing body odor generation is less than 7.5 mM, the generation of 2-nonenal cannot be effectively suppressed. On the other hand, even if it exceeds 150 mM, an effect corresponding thereto is expected. Not only can it cause an unpleasant feel when applied to the skin.

Moreover, this invention provides the composition for body odor removal in 2nd aspect.
The amino acid used in the composition for removing body odor of the present invention is a naturally occurring amino acid or a salt thereof, which may be extracted from a natural product according to a method known per se or chemically synthesized. Good. For example, L-cysteine hydrochloride monohydrate, L-cysteine (hereinafter referred to as “cysteine”) can be used. Amino acids are formulated into compositions as aqueous solutions for ease of application to the skin and for safety.

  Alanine, arginine, asparagine, aspartic acid, cysteine, N-acetylcysteine, cystine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine in the composition for removing body odor of the present invention The concentration of tryptophan, tyrosine, and valine is usually 0.1 to 50 mM, preferably 0.5 to 30 mM, more preferably 1.0 to 20 mM, and most preferably 1.5 to 15 mM. Alanine, arginine, asparagine, aspartic acid, cysteine, N-acetylcysteine, cystine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, When the tyrosine and valine concentrations are less than 0.1 mM, 2-nonenal cannot be effectively converted into odorless, and even if it exceeds 50 mM, the corresponding effect cannot be expected.

Furthermore, the present invention provides a composition for suppressing and removing body odor, which contains glucose and cysteine as described above. An unpleasant body odor can be more effectively eliminated by blending both components of glucose that suppresses the generation of 2-nonenal and cysteine that converts the generated 2-nonenal into an odorless substance.
Glucose and alanine, arginine, asparagine, aspartic acid, cysteine, N-acetylcysteine, cystine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, Phenylalanine, proline, serine, threonine, tryptophan, tyrosine, valine, and their blending concentrations are as described above.

  In addition to the essential components described above, a pH adjuster, preservative, chelating agent, stabilizer and the like as described below can be blended in the composition of the present invention depending on the intended use.

  Moreover, this invention provides the said composition used as a skin cosmetics additive, a skin external preparation additive, or a hair cosmetics additive in a 3rd form. Here, the skin cosmetic additive is preferably a deodorant additive.

  The components and blending amounts used in the skin cosmetic additive, skin external additive, or hair cosmetic additive of the present invention are the same as those described above for the antioxidant composition, and the aforementioned optional components are blended as appropriate. be able to.

  Further, the present invention provides a skin cosmetic, preferably deodorant, characterized in that, in the fourth form, each of the skin cosmetic additive, the skin external additive, or the hair cosmetic additive is contained. An agent, an external preparation for skin, or a hair cosmetic is provided.

  The final concentration of glucose in the skin cosmetic, skin external preparation, or hair cosmetic of the present invention is usually 7.5 to 150 mM, preferably 10 to 100 mM, more preferably 15 to 50 mM, and most preferably 20 to 30 mM. , Alanine, arginine, asparagine, aspartic acid, cysteine, N-acetylcysteine, cystine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, valine The concentration is usually 0.1 to 50 mM, preferably 0.5 to 30 mM, more preferably 1.0 to 20 mM, and most preferably 1.5 to 15 mM.

  In addition to the essential components described above for the composition, the skin cosmetic, external preparation for skin, or hair cosmetic of the present invention contains the following known components by a method known per se, a semi-solid, a solution, It can be used as a dosage form of a water-containing composition such as a suspension, a dispersion, or a water-in-oil type or an oil-in-water type emulsion.

Oils Higher alcohols such as cetyl alcohol, myristyl alcohol, oleyl alcohol, lauryl alcohol, cetostearyl alcohol, stearyl alcohol, isostearyl alcohol, 2-octyldodecanol, behenyl alcohol; lauric acid, myristic acid, palmitic acid, stearic acid, isostearic acid Higher fatty acids such as behenic acid, undecylenic acid, oleic acid, linoleic acid, linolenic acid, erucic acid, docosahexaenoic acid, eicosapentaenoic acid; octyldodecyl myristate, octyldodecyl palmitate, octyl isononanoate, isostearyl neopentanoate, myristine Ester oils such as isopropyl acid, glyceryl triisostearate; liquid paraffin, heavy liquid isoparaffin, light liquid iso Hydrocarbons such as paraffin, squalane, squalene, petrolatum, paraffin; waxes such as candelilla wax, carnauba wax, rice wax, beeswax, ceresin, paraffin wax, microcrystalline wax, petrolatum, polyethylene / polypropylene copolymer; palm oil, palm Oil, palm kernel oil, safflower oil, olive oil, castor oil, avocado oil, sesame oil, rosehip oil, meadow foam oil, mint oil, corn oil, rapeseed oil, sunflower oil, soybean oil, peanut oil, cocoa butter, shea Fats, hydrogenated coconut oil, hydrogenated castor oil, jojoba oil, hydrogenated jojoba oil and other vegetable oils; beef tallow, milk fat, horse fat, egg yolk oil, mink oil and other animal fats; whale wax, lanolin, liquid lanolin, reduced Lanolin, lanolin alcohol Lanolin; phospholipids such as lecithin, phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylglycerol, phosphatidylinositol, sphingomyelin, sphingophospholipid, phosphatidic acid, lysolecithin; palm oil fatty acid monoethanolamide (cocamide MEA), lauric acid mono Fatty acid alkanolamides such as isopropanolamide (lauramide MIPA); dimethicone (dimethylpolysiloxane), amino-modified silicone, cation-modified silicone, polyether-modified silicone, polyglycerin-modified silicone, alkyl-modified silicone, fatty acid-modified silicone, and the like; perfluoro Fluorine such as decane, perfluorooctane, perfluoropolyether Oil

Moisturizer / feel improver Glycerin, 1,3-butylene glycol, propylene glycol, hexylene glycol, diglycerin, polyglycerin, diethylene glycol, polyethylene glycol, ethylene glycol / propylene glycol copolymer, sorbitol, xylitol, erythritol, mannitol, Cyclodextrin, chitin, chitosan, hyaluronic acid, chondroitin, tuberose polysaccharide, urea, collagen, fish-derived collagen, gelatin, elastin, collagen-degrading peptide, hydrolyzed collagen, elastin-degrading peptide, keratin-degrading peptide, hydrolyzed keratin, chamomile extract , Daylily extract, silk extract, eucalyptus extract, ceramide

Surfactants Anionic surfactants such as sodium lauryl sulfate, triethanolamine lauryl sulfate, sodium laureth sulfate, triethanolamine laureth sulfate, sodium lauroyl sarcosine, lauroyl sarcosine triethanolamine, sodium lauroyl monoethanolamide succinate; laureth ( Polyoxyethylene lauryl ether), polyoxyethylene alkyl ether with polyoxyethylene addition number, polyoxyethylene hydrogenated castor oil, polyoxyethylene / polyoxypropylene cetyl ether, glyceryl stearate, glyceryl palm oil fatty acid, polyglyceryl distearate, sorbitan Monostearate, sucrose fatty acid ester, polyoxyethylene distearate, polyoxyethylene glycerol mono Nonionic surfactants such as stearate, coconut oil fatty acid diethanolamide (cocamide DEA), lauric acid monoethanolamide (lauramide MEA); cationic surfactants such as steartrimonium chloride and distearyldimonium chloride; Amphoteric surfactants such as lauryl betaine and cocamidopropyl betaine

Viscosity modifier Carrageenan, Caraya gum, Tragacanth gum, Xanthan gum, Xanthan gum, Pullulan, Gellan gum, Hydroxyethylcellulose, Hydroxypropylcellulose, Carboxymethylcellulose and its sodium salts, Methylhydroxypropylcellulose, Crystalline cellulose, Powdered cellulose, Polyvinylpidolidone, Polyvinyl alcohol , Polyoxyethylene / polyoxypropylene copolymer, (methacryloyloxyethylcarboxybetaine / alkyl methacrylate) copolymer, maleic acid copolymer, acrylic acid / methacrylic acid ester copolymer

Solvent / propellant ethanol, 2-propanol (isopropyl alcohol), butanol, isobutyl alcohol, propylene glycol, 1,3-butylene glycol, diethylene glycol, dipropylene glycol, dipropylene glycol monoethyl ether, benzyl alcohol, benzyloxyethanol, carbonic acid Propylene, dialkyl carbonate, acetone, ethyl acetate, N-methylpyrrolidone, toluene, fluorocarbon, LPG, dimethyl ether, carbon dioxide

Antioxidants, reducing agents, oxidizing agents Tocopherol, tocopherol acetate, BHT, BHA, ascorbic acid or its derivatives, thioglycolic acid, aqueous hydrogen peroxide, ammonium persulfate, sodium bromate, percarbonate

Antiseptic / antibacterial agent / chelating agent Methyl paraben, ethyl paraben, propyl paraben, sodium benzoate, triclosan, zinc pyrithione, benzalkonium chloride, benzethonium chloride, sorbic acid, chlorhexidine, halocarban, hinokitiol, cresol, thymol, parachlorophenol, Silver ion, ethylenediaminetetraacetate, sodium oxalate, sodium polyphosphate, sodium metaphosphate, phosphoric acid; sodium citrate, citric acid, alanine, dihydroxyethylglycine, gluconic acid, ascorbic acid, succinic acid, tartaric acid

pH adjuster Citric acid, sodium citrate, lactic acid, sodium lactate, glycolic acid, succinic acid, acetic acid, sodium acetate, malic acid, tartaric acid, fumaric acid, phosphoric acid, hydrochloric acid, sulfuric acid, monoethanolamine, diethanolamine, triethanolamine , Isopropanolamine, triisopropanolamine, sodium hydroxide, potassium hydroxide, aqueous ammonia,

Powder mica, talc, kaolin, sericite, montmorillonite, kaolinite, mica, titanium oxide, titanium mica,

UV absorbers Paraaminobenzoic acid, paraaminobenzoic acid monoglycerin ester, homomenthyl-N-acetylanthranylate, benzyl salicylate, p-isopropanolphenyl salicylate, octylcinnamate, 2,4-dihydroxybenzophenone, 3- (4'-methyl) Benzylidene) -d, l-camphor, octyl triazone, methyl anthranilate, oryzanol

Medicinal components Diphenhydramine hydrochloride, chlorpheniramine maleate, camphor, salicylic acid, resorcin, pyridoxine, menthol, methyl salicylate, citric acid, tartaric acid, lactic acid, tannic acid, superoxide dismutase, catalase, lysozyme chloride, lipase, papain, pancreatin , Protease

Next, the present invention will be described in more detail with reference to examples. However, these aspects are intended to explain the present invention, and the present invention is not limited thereto.
In addition, cysteine used in the following examples is all cysteine hydrochloride monohydrate unless otherwise specified.

Example 1
After culturing human keratinocytes (Kurashikibo Co., Ltd.) in 5, 20, 50 mM glucose aqueous solution for 4 days, active oxygen and MTT (cell viability) were measured. The numerical values were equalized by dividing active oxygen by the MTT value, and the value when the glucose concentration was 5 mM was shown as 100. Data are average values at n = 4.

  As a result, when the glucose concentration was 20 mM, the amount of active oxygen generated was the lowest value, and it was shown that the high concentration of glucose suppressed the generation of active oxygen causing 2-nonenal generation.

Example 2
In order to examine the mechanism of active oxygen suppression by glucose, human keratinocytes were cultured for 4 days in a 5 or 20 mM aqueous glucose solution, and then glucose-6-phosphate dehydrogenase (G6PD) activity in the pentose phosphate pathway was measured. The numerical values were equalized by dividing active oxygen by the amount of protein, and the value at 5 mM was shown as 100. Data are average values at n = 4.

  The first rate-limiting enzyme G6PD in the pentose phosphate cycle is the main source of NADPH and has recently been noted to be involved in the generation of active oxygen via NADPH oxidase. The above results suggested that the activity of G6PD was suppressed and glucose generation was inhibited by high concentration of glucose.

Example 3
The ability of cysteine to convert 2-nonenal, which is said to be the cause of aging odor, was examined. Using the following Sample, the ratio of 2-nonenal to the product in heavy methanol at room temperature was determined by 400 MHz nuclear magnetic resonance (hereinafter referred to as NMR).
The results are shown in Table 3.

Sample A (Cys 6mg): 7 mg 2-nonenal dissolved in 0.5 mL deuterated methanol.
And add 6 mg cysteine (not hydrochloride)
Things.
2-nonenal: cysteine = 1: 1 (molar ratio).
Sample B (Cys 12mg): 7 mg 2-nonenal dissolved in 0.5 mL deuterated methanol.
And add 12 mg cysteine (not hydrochloride)
What
2-nonenal: cysteine = 1: 2 (molar ratio).
Samples A and B were both stored at room temperature in glass containers (with NMR measurement tubes and lids).

その結果、システインにより２−ノネナールが減少することが判明した。

As a result, it was found that 2-nonenal was decreased by cysteine.

Example 4
Alanine, arginine, asparagine, aspartic acid, N-acetylcysteine, cystine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, valine, cysteine above As in the 2-nonenal reduction experiment by II, 2-nonenal (7 mg) and amino acid (1: 1 (molar ratio)) were dissolved in deuterated methanol, and the 2-nonenal residual rate after 24 hours was measured by NMR.

すべてのアミノ酸において、程度の差はあるが、２−ノネナールの減少効果が認められた。

In all amino acids, 2-nonenal was found to have a decreasing effect, although to some extent.

Example 5
The elimination effect of 2-nonenal odor by an aqueous solution containing glucose and cysteine was evaluated by olfaction of 4 adult healthy men. Spray 1 wt% 2-nonenal solution dissolved in ethanol into two 10cm x 5cm x 20cm plastic containers, and spray one of them with (20mM glucose + 3mM cysteine) mixed aqueous solution, Blind for 30 minutes The odors in each of the subsequent containers were directly sniffed and examined.
The results are shown in Table 4.

  It was found that when 2-nonenal alone was sprayed, an intense 2-nonenal odor was observed, whereas when it was sprayed with a mixture of glucose and cysteine, 2-nonenal odor was attenuated.

Example 6
Composition for suppressing body odor occurrence Ingredients Concentration glucose 20 mM
Cysteine 1.5 mM
Glutamate Na 5mM
water

Example 7
Composition for removing body odor Ingredients Concentration Glucose 20 mM
Cysteine 3.0 mM
Glutamate Na 7.5mM

water

Example 8
Composition for suppressing body odor generation and removing body odor Ingredients Concentration Glucose 25 mM
Cysteine 5.0 mM
Glutamate Na 7.5mM
Glycine 5.0 mM
water

Example 9
External preparation for skin Ingredients Concentration Glucose 20 mM
Cysteine 1.5 mM
Glutamate Na 5.0mM
water

Example 10
Skin cosmetics Ingredients Concentration glucose 20 mM
Cysteine 1.5 mM
Glutamate Na 7.5mM
water

Example 11
Hair cosmetics Ingredients Concentration Glucose 25 mM
Cysteine 5.0 mM
water

Example 12
Deodorant Ingredient Concentration Glucose 25 mM
Cysteine 3.0 mM
water

  ADVANTAGE OF THE INVENTION According to this invention, generation | occurrence | production of an aging odor and a scalp odor is effectively suppressed by the 2-nonenal generation | occurrence | production inhibitory effect which glucose has, and the 2-nonenal removal effect which cysteine has, and also the aging odor and scalp which were generated It is possible to provide a skin external preparation, skin cosmetic, and hair cosmetic that can remove odor.

Claims (6)

Water and glucose, as well as cysteine and one or two amino acids odor removing compositions you characterized by containing selected from N- acetylcysteine.   The composition according to claim 1, which contains 7.5 to 150 mM of glucose.   The composition according to claim 1 or 2, which contains 0.1 to 50 mM of amino acid as one amino acid concentration.   The composition according to any one of claims 1 to 3, which is a skin cosmetic additive.   The composition according to claim 4, which is a deodorant additive.   A deodorant comprising the deodorant additive according to claim 5.