JP5442687B2 - Lipolysis accelerator - Google Patents

Description

The present invention promotes the degradation of body fat, reducing fat systemic or topical relates to effective lipolysis promoting agent for the prevention and improvement of obesity.

  Obesity is caused by excessive intake of energy consumed and accumulated as neutral fat in fat cells, which is not only a factor that causes various diseases such as arteriosclerosis, but is not cosmetically desirable. There is a strong need for preventive improvements. However, in recent years, the proportion of obese people has been increasing year by year in Japan and other developed countries. Reasons include overeating, lack of exercise, and stress, but many people cannot continue because dietary restrictions and exercise therapy require a strong will and long duration.

  From such a background, various developments of a lipolysis promoter effective for the prevention and improvement of obesity have been made. For example, a lipolysis accelerator containing as an active ingredient an extract extracted from at least one material selected from the group consisting of pearl barley, barley, seiko, garnet cocoon, and puar tea can decompose fat accumulated in fat cells. It is known that it can promote and contribute to the suppression and prevention of obesity (see, for example, Patent Document 1). In addition, improvement of obesity by improving lipolysis of systemic or local adipose tissue by using a lipolysis accelerator containing one or more selected from the group consisting of genochocolate extract, peonies extract, assembly extract, and thyme extract It is also known that it is effective in suppressing or preventing obesity by preventing the increase of the same tissue (see, for example, Patent Document 2). Furthermore, a lipolysis promoter comprising as an active ingredient at least one selected from the group consisting of orange leaf, orange flower, dandelion leaf and caramaroot is an inhibitor of obesity, improves obesity, Or it is also known that it is useful for weight loss of the whole body adipose tissue (for example, refer to patent documents 3). Furthermore, a lipolysis promoter characterized by containing a plant of the family Pepperaceae as an active ingredient has a clear lipolysis promoting action in adipose tissue, and has an excellent effect in suppressing, preventing and improving obesity Is also known (see, for example, Patent Document 4). Furthermore, a lipolysis promoter characterized by containing a plant of thistle family as an active ingredient has an obvious lipolysis promoting action in adipose tissue, and has an excellent effect in suppressing, preventing and improving obesity Is also known (see, for example, Patent Document 5). Furthermore, it is also known that the fat decomposition accelerator containing banana peel or an extract thereof accelerates the decomposition of accumulated fat and can sufficiently suppress, prevent and improve obesity (see, for example, Patent Document 6). However, it cannot be said that these lipolysis promoters have sufficient effects, and some of them are concerned about side effects.

JP 2002-275078 A JP 2000-63237 A JP-A-11-228431 JP-A-8-245410 JP-A-8-301780 JP 2000-44482 A

  From the background as described above, further development of a lipolysis promoter that is sufficiently effective in preventing and improving obesity and that can be used safely is desired.

The present inventors pay attention to the increase and enormous number of fat cells that are the cause of obesity, and believe that obesity can be prevented and ameliorated by promoting the degradation of neutral fat in fat cells. the result of, found that the effect of promoting the decomposition of neutral lipids in adipocytes mosquito Rifo CALIFORNIA emissions Poppy (Esch scho lzia californi c a) , and completed the present invention.

That is, the present invention contains a plant of Californian poppy (Eschscholzia californica) and / or an extract thereof as an active ingredient, and includes tablets, capsules, granular preparations, lotions, gels, skin lotions, ointments, It is a lipolysis accelerator used in the form of a paste, a poultice, a plaster, a stick, a sheet, a bath, a body wash tablet or a syrup .

  The lipolysis promoter of the present invention and food and drink containing the same have an obvious lipolysis promoting action in adipose tissue, and have an excellent effect in preventing or improving obesity and improving obesity.

  Hereinafter, the present invention will be described in detail. The lipolysis promoter of the present invention, foods and drinks containing the same, methods for producing them, efficacy thereof, and the like are described below, but the present invention is not limited thereto.

  The lipolysis promoter of the present invention may contain each plant as an active ingredient as it is, but may contain a dried product or a powdered dried product as an active ingredient. Moreover, you may contain the extract extracted from each plant as an active ingredient. The plant extract may be a liquid extract extracted with water, various organic solvents, or various organic solvents including water, and the liquid extract is further dried by a normal drying method (for example, vacuum drying, freeze drying, etc.). Or it may be dried or concentrated by a concentration method or the like. Although the kind of organic solvent is not specifically limited, Ethanol, methanol, acetone, ethyl acetate, hexane, etc. are mentioned. Furthermore, you may perform refinement | purification processes, such as deodorizing and decoloring, to this plant extract in the range which does not affect the effect as needed.

  The lipolysis promoter of the present invention can be used in any form such as an oral preparation and an external preparation. Therefore, the plant extract may be formulated into a state that can be easily used as an ingestion agent, for example, by using an excipient or the like as appropriate. Furthermore, since the lipolysis promoter can be applied directly to the face and abdomen to locally reduce the fat in these areas, lotion, gel, skin lotion, ointment, paste, paste It can be used as an agent, plaster agent, stick agent, sheet agent, bath agent, body washing tablet and the like.

  The blending amount of the lipolysis promoter of the present invention varies depending on the addition form and administration form, but can be selected from a wide range. For example, in the case of an external preparation, it is preferably blended in the composition in an amount of 0.005% by weight (hereinafter simply referred to as%) or more, particularly 0.01 to 30% in terms of a solvent-extracted dried product. In the case of an oral preparation, it is preferably 0.01 to 10 g, particularly 0.05 to 3 g per day for an adult in terms of a solvent-extracted dried product.

  The above-described lipolysis promoter is blended in the food and drink of the present invention, and the food and drink that can be blended is not particularly limited, and various forms of confectionery such as gum, candy, and chocolate, health foods, beverages, and drinks , Seasonings, breads, noodles. The food and drink in the present invention may take the form of a health food, a functional food, or a food for specified health use that has an obesity prevention effect and an obesity improvement effect. It can also be used for general meals. And it is possible to improve obesity and lifestyle-related diseases derived from obesity by ingesting food and drink blended in this way. In this case, the blending amount of the lipolysis accelerator is preferably 0.0001 or more, and preferably 0.01 to 99% by weight in the food or drink in terms of the solvent-extracted dried product.

  Hereinafter, although a test example is given and this invention product is demonstrated still in detail, the range of this invention product is not restrict | limited by them.

Test example 1

  This test was conducted to obtain a plant extract from the plant body.

1) Test sample Eighteen kinds of plants shown in Table 1 were used.

2) Test method After drying the plant, adding 100 ml of 50% ethanol or water to 10 g of the dried product, performing an extraction treatment at 70 ° C. for 2 hours with stirring, filtering the obtained extract and concentrating under reduced pressure, Lyophilized to obtain a plant extract.

3) Test results The yield of each extract is shown in [Table 1].

Test example 2

  This test was conducted in order to examine the lipolysis promoting action of the plant extract obtained in Test Example 1.

1) Test sample The lyophilized plant extracts of 18 kinds of 50% ethanol extract and 7 kinds of water extract prepared in Test Example 1 were used alone or in combination of two or more kinds.

2) Test method (I) Cultivation of adipocytes MC3T3-G2 / PA6 cells, which are mouse-derived preadipocytes, are seeded on a 24 well plate at 5 × 10 ⁴ cells / well, and 10% fetal bovine serum (FBS) The cells were cultured at 37 ° C. in the presence of 5% CO _{2 in an} added α-MEM medium. Immediately before becoming confluent, the medium was replaced with a 10% FBSα-MEM medium supplemented with dexamethasone, 3-isobutyl-1-methylxanthine and glucose to induce differentiation into adipocytes. Cultivation was carried out for 8-9 days after induction, and the test was performed after adipocytes were matured.

(II) Lipolytic activity measurement method Discard the culture supernatant, wash the well with PBS (-), add lyophilized plant extract and Dulbecco's phosphate buffer containing 2% BSA, 4.5 g / L glucose, Incubated for 1 hour. The amount of the lyophilized plant extract was adjusted so that the final concentration in this reaction system would be 100 μg / ml when used alone or in combination of two or more. After the incubation, the supernatant was sampled, and the amount of glycerol that is a degradation product of fat was measured using Triglyceride E-Test Wako. The rate of promoting lipolysis is a relative value when the value of the control (when no lyophilized plant extract is added) represented by the following formula is 100%.

Lipolysis promotion rate (%) = [A / B] × 100
A: Free glycerol amount when the extract is added B: Free glycerol amount when the extract is not added

3) Test results The determination of the lipolysis promoting action was performed based on the rate of lipolysis promotion determined from the measured value of the amount of free glycerol produced by lipolysis. As shown in [Table 2], [Table 3] and [Table 4], when the test plant extract was added alone or in combination of two or more kinds, the lipolysis was clearly reduced as compared with the case of no addition. Has been promoted.

  EXAMPLES Hereinafter, although an Example is given and this invention product is demonstrated further in detail, the range of this invention product is not restrict | limited by them.

Chewing gum was prepared according to the following formulation.
Gum base 20.0 parts Sugar 55.0 parts Glucose 23.7 parts Softener 1.0 parts Oregon Mountain Grape 50% ethanol extract 0.8 parts

Chewing gum was prepared according to the following formulation.
Gum base 20.0 parts Xylitol 75.0 parts Reduced maltose 3.8 parts Softener 1.0 part Oregon mountain grape water extract 0.2 parts

Tablet confectionery was prepared according to the following formulation.
Sugar 75.0 parts Lactose 20.0 parts Glycerin fatty acid ester 0.2 parts Fragrance 0.4 parts Watercress 50% ethanol extract 0.1 parts Purified water 4.3 parts

Chocolate was prepared according to the following formulation.
Sugar 41.0 parts Cacao mass 15.0 parts Whole milk powder 25.0 parts Cocoa butter 18.0 parts Emulsifier 0.3 parts
Fragrance 0.4 parts Skunk Cabbage 50% Ethanol Extract 0.3 parts

A beverage was prepared according to the following formulation.
Fructose glucose beverage 5.00 parts Sugar 4.50 parts Acidulant 1.28 parts Fragrance 0.20 parts Kenzin 50% ethanol extract 0.02 parts Purified water 89.0 parts

A beverage was prepared according to the following formulation.
Orange fruit juice 85.25 parts Sugar 11.70 parts Citric acid 2.00 parts Fragrance 1.00 parts Mace 50% ethanol extract 0.05 parts

Ice cream was prepared according to the following formulation.
Fructose glucose liquid sugar 0.5 parts Sugar 8.7 parts Acidulant 1.2 parts Fragrance 0.3 parts Purified water 89.0 parts Stabilizer 0.2 parts Black cumin water extract 0.1 parts

Dog food was prepared according to the following recipe.
Corn 33.0 parts Wheat flour 35.0 parts Soybean meal 21.0 parts Rice bran (defatted) 5.5 parts Meat meal 5.0 parts Mineral mix 0.2 parts Ikoja 50% ethanol extract 0.3 parts

Capsules were prepared according to the following formulation.
Skunk cabbage water extract 50.0 parts Lactose 48.0 parts Magnesium stearate 2.0 parts The above ingredients were mixed uniformly, and the mixed powder was filled into hard capsules.

Tablets were prepared according to the following formulation.
Netol 50% ethanol extract 20.0 parts Granules for direct hitting 48.0 parts (magnesium aluminate metasilicate 20%, corn starch 30%, lactose 50%)
Crystalline cellulose 30.0 parts Magnesium stearate 2.0 parts The above ingredients were mixed uniformly, and the mixed powder was tableted into a tablet of 200 mg.

A syrup was prepared according to the following formulation.
Mace water extract 0.1 part simple syrup 30.0 parts purified water 69.9 parts The above plant extract was completely dissolved in purified water, and added with simple syrup and mixed to obtain a syrup agent.

Candy was prepared according to the following formulation.
California poppy water extract 0.2 parts Sugar 50.0 parts Minamata 35.3 parts Fragrance 0.5 parts Purified water 14.0 parts

Biscuits were prepared according to the following recipe.
Mace 50% ethanol extract 0.5 parts wheat flour 50.6 parts corn starch 5.1 parts sugar 12.7 parts margarine 6.5 parts salt 0.3 parts sodium carbonate 1.3 parts ammonium carbonate 0.5 parts soy lecithin 0 .3 parts whole egg 4.1 parts fragrance 0.3 parts purified water 17.8 parts The above materials were mixed to form a dough. After spreading, this was molded and baked in an oven to produce biscuits.

Claims (2)

It contains a plant of Californian poppy (Eschscholzia californica) and / or an extract thereof as an active ingredient, and includes tablets, capsules, granular preparations, lotions, gels, skin lotions, ointments, pastes, poultices, A lipolysis accelerator used in the form of a plaster, stick, sheet, bath, tablet for body washing, or syrup .   The lipolysis promoter according to claim 1, wherein the extract is an extract of water and / or an organic solvent.