JP4983125B2 - How to control blast disease - Google Patents
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- JP4983125B2 JP4983125B2 JP2006200377A JP2006200377A JP4983125B2 JP 4983125 B2 JP4983125 B2 JP 4983125B2 JP 2006200377 A JP2006200377 A JP 2006200377A JP 2006200377 A JP2006200377 A JP 2006200377A JP 4983125 B2 JP4983125 B2 JP 4983125B2
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Description
本発明は、イモチ病の防除方法に関する。 The present invention relates to a method for controlling blast disease.
イネのイモチ病を防除する活性を有する殺菌化合物として、メラニン生合成阻害作用を有する殺菌化合物が知られている。 As a bactericidal compound having an activity of controlling rice blast disease, bactericidal compounds having an inhibitory action on melanin biosynthesis are known.
当該殺菌化合物の単独の施用では、その施用量、施用方法等の条件によって、必ずしも十分なイモチ病防除効力を得ることができない場合があった。 In the single application of the bactericidal compound, there may be cases where it is not always possible to obtain a sufficient potato disease control effect depending on the conditions such as the application amount and the application method.
本発明者は、このような状況において鋭意検討した結果、本発明に至った。
即ち、本発明は以下の発明等を提供するものである。
［発明１］
メラニン生合成阻害作用を有する殺菌化合物および可給態珪酸を含有する珪酸質肥料を、育苗期から出穂期の間のイネの生育する場所に同時又は併用処理する工程を有するイモチ病の防除方法であり、
前記殺菌化合物と前記珪酸質肥料中の可給態珪酸との重量比が１：１５〜１：１４００の範囲であることを特徴とする防除方法。
［発明２］
可給態珪酸を含有する珪酸質肥料が０．５〜５０重量％の可給態珪酸を含有する珪酸質肥料であることを特徴とする発明１に記載された防除方法。
［発明３］
メラニン生合成阻害作用を有する殺菌化合物がピロキロンであることを特徴とする発明１又は２に記載された防除方法。
［発明４］
メラニン生合成阻害作用を有する殺菌化合物、及び、前記殺菌化合物に対して重量比で１５〜１４００の量の可給態珪酸を含有する珪酸質肥料を含有することを特徴とするイネのイモチ病防除用組成物。
As a result of intensive studies in such a situation, the present inventor has reached the present invention.
That is, the present invention provides the following inventions and the like.
[Invention 1]
A method for controlling rice blast disease having a step of simultaneously or simultaneously treating a siliceous fertilizer containing a sterilizing compound having an inhibitory action on melanin biosynthesis and available silicic acid at a place where rice grows from the seedling stage to the heading stage. Yes,
The pest control method, wherein the weight ratio of the sterilizing compound to the available silicic acid in the siliceous fertilizer is in the range of 1:15 to 1: 1400.
[Invention 2]
The control method described in the invention 1, wherein the siliceous fertilizer containing available silicic acid is a siliceous fertilizer containing 0.5 to 50 wt% of available silicic acid.
[Invention 3]
The antibacterial method described in the invention 1 or 2, wherein the bactericidal compound having an inhibitory action on melanin biosynthesis is pyroxylone.
[Invention 4]
Bacterial compound having inhibitory action on melanin biosynthesis, and rice blast disease control comprising siliceous fertilizer containing available silicic acid in an amount of 15 to 1400 by weight with respect to the bactericidal compound Composition.
本発明により、イモチ病を効果的に防除することができる。 According to the present invention, blast disease can be effectively controlled.
本発明におけるイネのイモチ病は、水稲病害において防除すべき重要な病害とされており、イネの葉に発生するイモチ病は葉イモチ病、イネの穂に発生するイモチ病は穂イモチ病と呼ばれている。特に穂イモチ病は収穫対象である籾を不稔化または不完全米化させることから、収穫量に重大な影響を及ぼす。また葉イモチ病は、発病株の生育を抑制し、出穂を遅らせるため、有効穂数及び一穂当りの精玄米重が減少するという被害をもたらす。本発明の方法は、育苗期から出穂期の間に行うことにより、葉イモチ病、更には穂イモチ病を防除することができる。 Rice blast disease in the present invention is considered to be an important disease to be controlled in rice plant diseases, rice blast disease occurring in rice leaves is called leaf potato disease, and rice blast disease occurring in rice ears is called panic disease. It is. In particular, potato blast disease causes sterility or incomplete rice production of the culm that is the target of harvesting, and thus has a significant effect on the yield. In addition, leaf blast disease suppresses the growth of the diseased strain and delays heading, resulting in a decrease in the number of effective ears and the weight of the polished rice per ear. By carrying out the method of the present invention between the seedling raising stage and the heading stage, it is possible to control leaf blast disease and, further, ear blast disease.
本発明におけるメラニン生合成阻害作用を有する殺菌化合物（以下、本殺菌化合物と記す。）とは、テトラヒドロキシナフタレンをジヒドロキシナフタレンに変換するのに関与しているレダクターゼおよび／またはデヒドラターゼ酵素のいずれかを阻害することによって、メラニンのイン・ビボ合成を減じることができる化合物であり、具体的にはピロキロン（ｐｙｒｏｑｕｉｌｏｎ、１，２，５，６−テトラヒドロピロロ〔３，２，１−ｉｊ〕キノリン−４−オン）、ジクロシメット（ｄｉｃｌｏｃｙｍｅｔ、（ＲＳ）−２−シアノ−Ｎ−〔（Ｒ）−１−（２，４−ジクロロフェニル）エチル〕−３，３−ジメチルブチラミド）、カルプロパミド（ｃａｒｐｒｏｐａｍｉｄ、２，２−ジクロロ−Ｎ−〔１−（４−クロロフェニル）エチル〕−１−エチル−３−メチルシクロプロパンカルボキサミド）、フサライド（ｐｈｔｈａｌｉｄｅ、４，５，６，７−テトラクロルフタリド）、クロベンチアジオン（ｃｈｌｏｂｅｎｔｈｉａｚｉｏｎｅ、４−クロロ−３−メチル−１，３−ベンゾチアゾール−２（３Ｈ）−オン）、トリシクラゾール（ｔｒｉｃｙｃｌａｚｏｌｅ、５−メチル−１，２，４−トリアゾロ〔３，４−ｂ〕ベンゾチアゾール）、フェノキサニル（ｆｅｎｏｘａｎｉｌ、Ｎ−（２，３−ジクロロ−４−ヒドロキシフェニル）−１−メチルシクロヘキサンカルボキサミド）が挙げられる。 The bactericidal compound having an inhibitory action on melanin biosynthesis in the present invention (hereinafter referred to as the present bactericidal compound) refers to either a reductase and / or a dehydratase enzyme involved in converting tetrahydroxynaphthalene to dihydroxynaphthalene. It is a compound that can reduce the in vivo synthesis of melanin by inhibiting, specifically, pyroquilon (1,2,5,6-tetrahydropyrrolo [3,2,1-ij] quinoline-4 -One), diclocimet (RS) -2-cyano-N-[(R) -1- (2,4-dichlorophenyl) ethyl] -3,3-dimethylbutyramide), carpropamide, 2, 2-Dichloro-N- [1- (4-chlorophenyl) ethyl] 1-ethyl-3-methylcyclopropanecarboxamide), fusalide (phthalide, 4,5,6,7-tetrachlorophthalide), clobenchazione, 4-chloro-3-methyl-1,3-benzo Thiazol-2 (3H) -one), tricyclazole, 5-methyl-1,2,4-triazolo [3,4-b] benzothiazole, phenoxanil, N- (2,3-dichloro-4 -Hydroxyphenyl) -1-methylcyclohexanecarboxamide).
本発明における可給態珪酸を含有する珪酸質肥料とは、珪酸カルシウム、珪酸カリウム、シリカゲル、シリカヒドロゲル等の珪酸成分を含有する肥料を意味し、珪酸質肥料中の可給態珪酸が本殺菌化合物の量を１として重量比で１５〜１４００の範囲の量である珪酸質肥料（以下、本珪酸質肥料と記す。）である。
本珪酸質肥料における可給態珪酸の量は、３０℃の１Ｍ酢酸ナトリウム緩衝液に溶解する珪酸（SiO2）の量を意味する。本珪酸質肥料は、可給態珪酸を通常０．５〜５０重量％、好ましくは３〜３０重量％含有している。可給態珪酸の量は、具体的には以下に示すような分析法により本珪酸質肥料における含有量を分析することができる。
・可給態珪酸の分析法
〔試料液の調製〕
分析試料１ｇを２５０ｍｌのメスフラスコに正確にとり、３０℃の１Ｍ酢酸ナトリウム緩衝液１５０ｍｌを加え、１分間３０〜４０回回転の振り混ぜ機で１時間振り混ぜたのち（浸出中は３０℃に保つこと）、速やかに常温に戻し標線まで水を加えて直ちに乾燥ろ紙でろ過する。
〔定量〕
試料液を０．５ｍｌとり、純水８ｍｌを添加する。次にモリブデン酸アンモニウムの塩酸酸性溶液１ｍｌを添加して室温で２０分静置する。酒石酸１ｍｌを添加して室温で３分間静置する。アスコルビン酸粉末を小さじ一杯添加して振り混ぜる。室温で５分間静置した後に、ＳＰＡＤ ＳＦＰ−２土壌作物体総合分析装置（富士平工業株式会社製）を用いて下記の条件で測定を行う。
測定ランプ：ＰＨＯＴＯ
モード：ＳＯＩＬ
項目：ＳｉＯ2
波長：８１０ｎｍ
The siliceous fertilizer containing available silicic acid in the present invention means a fertilizer containing silicic acid components such as calcium silicate, potassium silicate, silica gel, silica hydrogel, etc., and the available silicic acid in the siliceous fertilizer is sterilized. A siliceous fertilizer (hereinafter referred to as the present siliceous fertilizer) having an amount of the compound of 1 and a weight ratio in the range of 15 to 1400.
The amount of available silicic acid in the siliceous fertilizer means the amount of silicic acid (SiO 2 ) dissolved in a 1 M sodium acetate buffer at 30 ° C. The siliceous fertilizer usually contains available silicic acid in an amount of 0.5 to 50% by weight, preferably 3 to 30% by weight. Specifically, the amount of available silicic acid can be analyzed for the content of the siliceous fertilizer by the following analytical method.
・ Analytical method of available silica (Preparation of sample solution)
Accurately place 1 g of analytical sample in a 250 ml volumetric flask, add 150 ml of 1 M sodium acetate buffer solution at 30 ° C., shake for 1 hour with a shaker rotating 30 to 40 times for 1 minute (keep at 30 ° C. during leaching) ) Immediately return to room temperature, add water up to the marked line, and immediately filter with dry filter paper.
[Quantitative]
Take 0.5 ml of the sample solution and add 8 ml of pure water. Next, 1 ml of an acidic solution of ammonium molybdate in hydrochloric acid is added and left at room temperature for 20 minutes. Add 1 ml of tartaric acid and let stand at room temperature for 3 minutes. Add a teaspoon of ascorbic acid powder and shake. After leaving still at room temperature for 5 minutes, it measures on the following conditions using a SPAD SFP-2 soil crop body comprehensive analyzer (made by Fujihira Kogyo Co., Ltd.).
Measuring lamp: PHOTO
Mode: SOIL
Item: SiO 2
Wavelength: 810nm
本発明のイモチ病の防除方法において、本殺菌化合物及び本珪酸質肥料は育苗期から出穂期の間のイネの生育する場所に同時処理又は併用処理することにより用いられるが、本殺菌化合物はそのままで施用しても、公知の剤型に製剤化された本殺菌化合物を含有する製剤として施用してもよい。
本殺菌化合物を含有する製剤は、例えば固体担体、液体担体等の各種担体と混合し、必要であればさらに添加剤として界面活性剤、その他の製剤用補助剤を加えて製造され、例えば、乳剤、水和剤、水中懸濁剤、水中乳濁剤、粒剤、粉剤等の製剤が挙げられる。これらの製剤には、有効成分として本殺菌化合物を、通常、重量比で0.001％〜９５％含有する。また、本殺菌化合物及び本珪酸質肥料を同時に含有する粒状物として製剤化することもできる。
このような製剤化に際し用いられる固体担体としては、例えば粘土類（カオリンクレー、ベントナイト、フバサミクレー、酸性白土等）、タルク類、珪藻土、合成含水酸化珪素、セラミック、その他の無機鉱物（セリサイト、石英、硫黄、活性炭、水和シリカ等）等の微粉末あるいは粒状物等があげられ、液体担体としては、例えば水、アルコール類（メタノール、エタノール等）、ケトン類（アセトン、メチルエチルケトン等）、芳香族炭化水素類（ベンゼン、トルエン、キシレン、エチルベンゼン、メチルナフタレン等）、脂肪族炭化水素類（ヘキサン、シクロヘキサン、灯油、軽油等）、エステル類（酢酸エチル、酢酸ブチル等）、ニトリル類（アセトニトリル、イソブチロニトリル等）、エーテル類（ジイソプロピルエーテル、ジオキサン等）、酸アミド類（Ｎ、Ｎ−ジメチルホルムアミド、Ｎ、Ｎ−ジメチルアセトアミド等）、ハロゲン化炭化水素類（ジクロロメタン、トリクロロエタン、四塩化炭素等）、ジメチルスルホキシド、大豆油、綿実油等の植物油等があげられる。
界面活性剤としては、例えばアルキル硫酸エステル塩類、アルキルスルホン酸塩及びアルキルアリールスルホン酸塩等のアニオン系界面活性剤、アルキルアリールアルコール類およびそのポリオキシアルキレン付加化合物、アルキルアルコール類およびそのポリオキシアルキレン付加化合物、多価アルコールおよびそのエステル類ならびに糖類等のノニオン系界面活性剤があげられる。
固着剤や分散剤等の製剤用補助剤としては、例えばカゼイン、ゼラチン、多糖類（でんぷん、アラビアガム、セルロース誘導体、アルギン酸等）、リグニン誘導体、ベントナイト、糖類、合成水溶性高分子（ポリビニルアルコール、ポリビニルピロリドン、ポリアクリル酸類等）等があげられ、安定剤としては、例えばＰＡＰ（酸性リン酸イソプロピル）、ＢＨＴ（２、６−ジ−tert−ブチル−４−メチルフェノール）、ＢＨＡ（２−tert−ブチル−４−メトキシフェノールと３−tert−ブチル−４−メトキシフェノールとの混合物）、植物油、鉱物油、界面活性剤、脂肪酸またはそのエステル等があげられる。
In the method for controlling rice blast disease of the present invention, the present bactericidal compound and the present siliceous fertilizer are used by simultaneous treatment or combined treatment at the place where rice grows from the seedling stage to the heading stage, but the bactericidal compound is used as it is. Or may be applied as a preparation containing the present bactericidal compound formulated into a known dosage form.
Preparations containing this bactericidal compound are produced by mixing with various carriers such as solid carriers and liquid carriers, and if necessary, further adding surfactants and other formulation adjuvants as additives. , Wettable powder, suspension in water, emulsion in water, granule, powder and the like. These preparations usually contain the present bactericidal compound as an active ingredient in a weight ratio of 0.001% to 95%. Moreover, it can also formulate as a granular material which contains this disinfection compound and this siliceous fertilizer simultaneously.
Examples of solid carriers used in the formulation include clays (kaolin clay, bentonite, fubasami clay, acid clay), talc, diatomaceous earth, synthetic silicon hydroxide, ceramics, and other inorganic minerals (sericite, quartz). , Sulfur, activated carbon, hydrated silica, etc.) and the like, and liquid carriers include, for example, water, alcohols (methanol, ethanol, etc.), ketones (acetone, methyl ethyl ketone, etc.), aromatic Hydrocarbons (benzene, toluene, xylene, ethylbenzene, methylnaphthalene, etc.), aliphatic hydrocarbons (hexane, cyclohexane, kerosene, gas oil, etc.), esters (ethyl acetate, butyl acetate, etc.), nitriles (acetonitrile, iso Butyronitrile, etc.), ethers (diisopropyl ether, geo) Sun, etc.), acid amides (N, N-dimethylformamide, N, N-dimethylacetamide, etc.), halogenated hydrocarbons (dichloromethane, trichloroethane, carbon tetrachloride, etc.), dimethyl sulfoxide, soybean oil, cottonseed oil and other vegetable oils Etc.
Examples of the surfactant include anionic surfactants such as alkyl sulfates, alkyl sulfonates and alkylaryl sulfonates, alkylaryl alcohols and polyoxyalkylene addition compounds thereof, alkyl alcohols and polyoxyalkylenes thereof. Nonionic surfactants such as addition compounds, polyhydric alcohols and esters thereof, and saccharides can be mentioned.
Examples of adjuvants for preparations such as fixing agents and dispersants include casein, gelatin, polysaccharides (starch, gum arabic, cellulose derivatives, alginic acid, etc.), lignin derivatives, bentonite, saccharides, synthetic water-soluble polymers (polyvinyl alcohol, Polyvinyl pyrrolidone, polyacrylic acids, etc.), and examples of the stabilizer include PAP (isopropyl acid phosphate), BHT (2,6-di-tert-butyl-4-methylphenol), BHA (2-tert). -Mixtures of butyl-4-methoxyphenol and 3-tert-butyl-4-methoxyphenol), vegetable oils, mineral oils, surfactants, fatty acids or esters thereof.
本発明のイモチ病の防除方法において、本珪酸質肥料はそのままで施用しても、他の種類の肥料も含有する肥料組成物として施用してもよく、本珪酸質肥料は粉状、粒状、ペースト状、液状等の形状であってもよい。該肥料組成物に含有する他の種類の肥料としては、例えば窒素、リン、カリウム、マグネシウム、カルシウム、マンガン、ホウ素、鉄など水稲が要求する種々の元素を含有するもの肥料であり、具体的には、尿素、硝酸アンモニウム、硝酸苦土アンモニウム、塩化アンモニウム、硫酸アンモニウム、リン酸アンモニウム,硝酸ソーダ、硝酸カルシウム、硝酸カリウム、石灰窒素、ホルムアルデヒド加工尿素肥料（CDU）、イソブチルアルデヒド加工尿素肥料（IBDU）グアニール尿素（GU）などの窒素質肥料、過リン酸石灰、重過燐酸石灰、熔成リン肥、腐植酸リン肥、焼成リン肥、重焼リン、苦土過リン酸、ポリリン酸アンモニウム、メタリン酸カリウム、メタリン酸カルシウム、苦土リン酸、硫リン安、リン硝安カリウム、塩リン安などのリン酸質肥料、塩化カリウム、硫酸カリウム、硫酸カリソーダ、硫酸カリ苦土、重炭酸カリウム、リン酸カリウムなどのカリウム質肥料、硫酸マグネシウム、塩化マグネシウムなどのマグネシウム質肥料、生石灰、消石灰、炭酸カルシウムなどのカルシウム質肥料、硫酸マンガン、硫酸苦土マンガン、鉱さいマンガンなどのマンガン質肥料、ホウ酸、ホウ酸塩などのホウ素質肥料などの肥料取締法に定められる普通肥料をあげることができる。 In the method for controlling blast disease according to the present invention, the present siliceous fertilizer may be applied as it is or may be applied as a fertilizer composition containing other types of fertilizers. It may be in the form of a paste or liquid. Examples of other types of fertilizers contained in the fertilizer composition include fertilizers containing various elements required by paddy rice such as nitrogen, phosphorus, potassium, magnesium, calcium, manganese, boron, and iron. Urea, ammonium nitrate, ammonium nitrate, ammonium nitrate, ammonium sulfate, ammonium phosphate, sodium nitrate, calcium nitrate, potassium nitrate, lime nitrogen, formaldehyde processed urea fertilizer (CDU), isobutyraldehyde processed urea fertilizer (IBDU) GU) nitrogenous fertilizer, superphosphate lime, heavy superphosphate lime, molten phosphorus fertilizer, humic phosphate fertilizer, calcined phosphorous fertilizer, heavy calcined phosphorous, bitumen hyperphosphate, ammonium polyphosphate, potassium metaphosphate, Phosphoric acids such as calcium metaphosphate, maternal phosphoric acid, ammonium sulfate, potassium ammonium phosphate, ammonium phosphate Fertilizer, potassium chloride, potassium sulfate, potassium sulfate, potassium sulfate, potassium bicarbonate, potassium phosphate such as potassium phosphate, magnesium fertilizer such as magnesium sulfate, magnesium chloride, calcium such as quick lime, slaked lime, calcium carbonate Ordinary fertilizers stipulated in the fertilizer control law such as fertilizers such as fertilizer, manganese sulfate such as manganese sulfate, manganese sulfate sulfate and manganese, and boron fertilizer such as boric acid and borate.
本発明のイモチ病の防除方法において、本殺菌化合物及び本珪酸質肥料をイネの生育する場所に同時処理又は併用処理する場合、好ましくは粒状物（粒剤）の形態で本殺菌化合物及び本珪酸質肥料を施用することが好ましく、該粒状物が本殺菌化合物を含有する粒状物と本珪酸質肥料を含有する粒状物との混合物であってもよい。 In the method for controlling blast disease according to the present invention, when the bactericidal compound and the siliceous fertilizer are simultaneously or simultaneously treated at the place where rice grows, the bactericidal compound and the silicic acid are preferably used in the form of granules (granules). It is preferable to apply a fertilizer, and the granular material may be a mixture of a granular material containing the present bactericidal compound and a granular material containing the present siliceous fertilizer.
本発明のイモチ病の防除方法において、本殺菌化合物及び本珪酸質肥料を施用するイネの生育する場所としては、イネ苗を育苗する育苗箱、育苗後のイネ苗を定植する水田が挙げられ、同時処理又は併用処理の方法としては、例えば育苗箱の覆土への混和及び表面散布処理、イネ苗の定植前の水田土壌への混和処理、定植後の水田への側条処理及び水面処理が挙げられる。
本発明において、本殺菌化合物と本珪酸質肥料中の可給態珪酸との重量比は、育苗箱の覆土への混和及び表面散布処理においては１：１５〜１：３００の範囲が好ましく、イネ苗の定植前の水田土壌への混和処理並びに定植後の水田への側条処理及び水面処理においては１：３００〜１：１４００の範囲が好ましい。
本発明の方法において、本殺菌化合物の種類、処理時期等により変化させることができるが、水田土壌への混和処理並びに側条処理又は水面処理において本殺菌化合物は通常０．１〜５０００ｇ／１０ａ、好ましくは１〜５００ｇ／１０ａの量で使用し、育苗箱の覆土混和処理又は表面散布処理において本殺菌化合物は通常０．０１ｇ〜５００ｇ／１箱、好ましくは０．１〜５０ｇ／１箱の量で使用する。
In the method for controlling rice blast disease of the present invention, examples of the place where rice is to be applied with the present bactericidal compound and the present siliceous fertilizer include a seedling box for raising rice seedlings, a paddy field for planting rice seedlings after raising seedlings, Examples of the simultaneous treatment or combined treatment include mixing of seedling boxes into the cover soil and surface spraying treatment, mixing treatment of rice seedlings in paddy soil before planting, lateral treatment and water surface treatment of paddy fields after planting. It is done.
In the present invention, the weight ratio of the present bactericidal compound to the available silicic acid in the siliceous fertilizer is preferably in the range of 1:15 to 1: 300 in the mixing of the seedling box into the covering soil and the surface spraying treatment. The range of 1: 300 to 1: 1400 is preferable in the admixing treatment to the paddy soil before planting the seedlings and the side strip treatment and water surface treatment to the paddy field after planting.
In the method of the present invention, the sterilizing compound can be changed depending on the kind of the sterilizing compound, the treatment time, etc., but the sterilizing compound is usually 0.1 to 5000 g / 10a in the mixing treatment to the paddy soil and the side strip treatment or water surface treatment Preferably, it is used in an amount of 1 to 500 g / 10a, and the present bactericidal compound is usually in an amount of 0.01 g to 500 g / 1 box, preferably 0.1 to 50 g / 1 box in the soil cover mixing treatment or surface spraying treatment of the seedling box. Used in.
本発明のイモチ病防除用組成物としては、例えば(1)本殺菌化合物を含有する粒状物と本珪酸質肥料を含有する粒状物との混合物、(2)粒状の本珪酸質肥料の表面に、本殺菌化合物の液状製剤を吹き付け乾燥させた粒状物、(3)本殺菌化合物、本珪酸質肥料及び固体担体、更に必要に応じて粘結剤を添加し造粒した粒状物等が挙げられる。 Examples of the composition for controlling blast disease according to the present invention include (1) a mixture of a granular material containing the present bactericidal compound and a granular material containing the present siliceous fertilizer, and (2) a surface of the granular present siliceous fertilizer. , Granular products obtained by spraying and drying the liquid preparation of the present bactericidal compound, (3) the present bactericidal compound, the present silicic acid fertilizer and solid carrier, and further granulated particles added with a binder if necessary. .
本発明を以下の実施例によって更に詳細に説明するが、本発明はこれらの例に限定されるものではない。 The present invention will be described in more detail with reference to the following examples, but the present invention is not limited to these examples.
試験例１（育苗箱処理）
縦2cm、横2cm、深さ4.5cmのプラスチック容器で2週間育苗したイネ（日本晴）に、表１記載の本殺菌化合物及び／又は本珪酸質肥料を施用した。施用後1日目に、開口面積が1/10000aのポットにイネを定植した。定植後21日目に、イモチ病の罹病苗を前記のポットの周辺に配置した。定植後21〜31日目までの間は、農業用透明フィルムにて縦2m、横1.9m、高さ1.2mに区切った温室内の空間にてイネを栽培した。尚、夜間（17時〜8時）は該空間内を水蒸気発生装置にて湿度100％に加湿した。定植後31日目に、完全展開した上位2葉における病斑の面積率を目視にて観察し、下記に示す基準にて防除指数を算出した。尚、試験は３反復で行った。結果を表１に記す。
〔防除指数〕
０：無処理区の病斑面積率と同等〜4/5以上
１：無処理区の病斑面積率の3/5〜4/5
２：無処理区の病斑面積率の2/5〜3/5
３：無処理区の病斑面積率の1/5〜2/5
４：無処理区の病斑面積率の1/5以下
Test example 1 (nursing box processing)
This bactericidal compound and / or this siliceous fertilizer shown in Table 1 was applied to rice (Nipponbare) grown in a plastic container having a length of 2 cm, a width of 2 cm, and a depth of 4.5 cm for 2 weeks. On the first day after application, rice was planted in a pot with an opening area of 1 / 10000a. On the 21st day after planting, the seedlings of blast disease were placed around the pot. From the 21st to the 31st day after planting, rice was cultivated in the space in the greenhouse divided into 2m in length, 1.9m in width, and 1.2m in height with a transparent film for agriculture. Incidentally, at night (17:00 to 8:00), the interior of the space was humidified to a humidity of 100% with a steam generator. On the 31st day after planting, the area ratio of lesions in the fully expanded upper two leaves was visually observed, and the control index was calculated according to the criteria shown below. The test was repeated 3 times. The results are shown in Table 1.
[Control index]
0: Equivalent to the lesion area ratio in the untreated section ~ 4/5 or more 1: 3/5 to 4/5 of the lesion area ratio in the untreated section
2: 2/5 to 3/5 of lesion area rate in untreated area
3: 1/5 to 2/5 of lesion area rate in untreated area
4: Less than 1/5 of the lesion area ratio in the untreated area
*2：くみあいホウ素入り20.0けい酸加里肥料特号（開発肥料社製）を使用した。
可給態珪酸の含量は5.0%であった。
*3：くみあい粒状珪酸苦土石灰2号（日清鋼業社製）を使用した。
可給態珪酸の含量は17.0%であった。
* 2: The 20.0 silicate potassium fertilizer special number (manufactured by Development Fertilizer) containing Kumiai Boron was used.
The content of available silicic acid was 5.0%.
* 3: Kumiai granular silicic acid dough lime No. 2 (Nisshin Steel Industry Co., Ltd.) was used.
The content of available silicic acid was 17.0%.
試験例２（定植時施用）
表２記載の本珪酸質肥料を混和した土壌を開口面積が1/10000aのポットに詰め、イネ（日本晴）を定植した後、表２記載の本殺菌化合物を水面施用した。定植後21日目に、イモチ病の罹病苗を前記のポットの周辺に配置した。定植後21〜31日目までの間は、農業用透明フィルムにて縦2m、横1.9m、高さ1.2mに区切った温室内の空間にてイネを栽培した。尚、夜間（17時〜8時）は該空間内を水蒸気発生装置にて湿度100％に加湿した。定植後31日目に、完全展開した上位2葉における病斑の面積率を目視にて観察し、上記に示した基準にて防除指数を算出した。尚、試験は３反復で行った。結果を表２に記す。
Test example 2 (application at the time of planting)
The soil mixed with the present siliceous fertilizer described in Table 2 was packed in a pot having an opening area of 1 / 10000a, and after planting rice (Nihonbare), the present bactericidal compound described in Table 2 was applied to the surface. On the 21st day after planting, the seedlings of blast disease were placed around the pot. From the 21st to the 31st day after planting, rice was cultivated in the space in the greenhouse divided into 2m in length, 1.9m in width, and 1.2m in height with a transparent film for agriculture. Incidentally, at night (17:00 to 8:00), the interior of the space was humidified to a humidity of 100% with a steam generator. On the 31st day after planting, the area ratio of lesions in the top 2 fully expanded leaves was visually observed, and the control index was calculated according to the criteria shown above. The test was repeated 3 times. The results are shown in Table 2.
*2：くみあいホウ素入り20.0けい酸加里肥料特号（開発肥料社製）を使用した。
可給態珪酸の含量は5.0%であった。
*3：くみあい粒状珪酸苦土石灰2号（日清鋼業社製）を使用した。
可給態珪酸の含量は17.0%であった。
* 2: The 20.0 silicate potassium fertilizer special number (manufactured by Development Fertilizer) containing Kumiai Boron was used.
The content of available silicic acid was 5.0%.
* 3: Kumiai granular silicic acid dough lime No. 2 (Nisshin Steel Industry Co., Ltd.) was used.
The content of available silicic acid was 17.0%.
試験例３（水面施用）
開口面積が1/10000aのポットにイネを定植し、栽培した。定植後14日目に、表３記載の本殺菌化合物及び／又は本珪酸質肥料を水面施用した。定植後42日目に、イモチ病の罹病苗を前記のポットの周辺に配置した。定植後42〜71日目までの間は、農業用透明フィルムにて縦2m、横1.9m、高さ1.2mに区切った温室内の空間にてイネを栽培した。尚、夜間（17時〜8時）は該空間内を水蒸気発生装置にて湿度100％に加湿した。定植後71日目に、イネを下記に示す基準で目視観察して発病度を求め、該発病度を基に防除指数を算出した。尚、試験は３反復で行った。結果を表３に示す。
〔発病度〕
発病度＝（Σ（全調査穂数×発病指数）／（全調査穂数×５））×１００
発病指数は０（発病なし。）、１（籾が変色しているが、枝梗での発病なし。）、２（枝梗の1/3まで発病。）、３（枝梗の1/3〜2/3発病。）、４（枝梗の2/3以上が発病）及び５（穂首まで発病。）の５段階で分類した。
〔防除指数〕
０：無処理区の発病度と同等〜4/5以上
１：無処理区の発病度の3/5〜4/5
２：無処理区の発病度の2/5〜3/5
３：無処理区の発病度の1/5〜2/5
４：無処理区の発病度の1/5以下
Test example 3 (water surface application)
Rice was planted and cultivated in a pot with an open area of 1 / 10000a. On the 14th day after planting, the fungicidal compound and / or the siliceous fertilizer shown in Table 3 were applied to the water surface. On day 42 after planting, seedlings of blast disease were placed around the pot. From 42 to 71 days after planting, rice was cultivated in a space in a greenhouse divided into 2 m in length, 1.9 m in width, and 1.2 m in height with a transparent film for agriculture. Incidentally, at night (17:00 to 8:00), the interior of the space was humidified to a humidity of 100% with a steam generator. On day 71 after planting, rice was visually observed according to the following criteria to determine the disease severity, and a control index was calculated based on the disease severity. The test was repeated 3 times. The results are shown in Table 3.
[Severity]
Disease incidence = (Σ (total number of ears surveyed × attack index) / (total number of ears surveyed × 5)) × 100
Disease index is 0 (no disease onset), 1 (carp is discolored, but no disease on branch infarction), 2 (onset to 1/3 of branch infarction), 3 (1/3 on branch infarction) The disease was classified into 5 levels, 4 (onset of 2/3 or more of branch infarcts) and 5 (onset to the neck).
[Control index]
0: Equivalent to the severity of the untreated zone ~ 4/5 or more 1: 3/5 to 4/5 of the severity of the untreated zone
2: 2/5 to 3/5 of disease severity in untreated areas
3: 1/5 to 2/5 of disease severity in untreated areas
4: Less than 1/5 of disease severity in untreated areas
*2：くみあいホウ素入り20.0けい酸加里肥料特号（開発肥料社製）を使用した。
可給態珪酸の含量は5.0%であった。
*3：くみあい粒状珪酸苦土石灰2号（日清鋼業社製）を使用した。
可給態珪酸の含量は17.0%であった。
* 2: The 20.0 silicate potassium fertilizer special number (manufactured by Development Fertilizer) containing Kumiai Boron was used.
The content of available silicic acid was 5.0%.
* 3: Kumiai granular silicic acid dough lime No. 2 (Nisshin Steel Industry Co., Ltd.) was used.
The content of available silicic acid was 17.0%.
本発明のイモチ病の防除方法は、育苗期から出穂期に同時又は併用処理することにより、イモチ病を効果的に防除することができる。
In the method for controlling blast disease according to the present invention, blast disease can be effectively controlled by performing simultaneous or combined treatment from the seedling stage to the heading stage.
Claims (3)
ピロキロンと前記珪酸質肥料中の可給態珪酸との重量比が１：１５〜１：１４００の範囲であることを特徴とする防除方法。 A method for controlling rice blast disease comprising a step of simultaneously or jointly treating siliceous fertilizer and pyrokilone containing available silicic acid at a place where rice grows from the seedling stage to the heading stage, wherein the available silicic acid Is a fertilizer containing calcium silicate or potassium silicate ,
A control method , wherein the weight ratio of pyroxylone to available silicic acid in the siliceous fertilizer is in the range of 1:15 to 1: 1400.
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