JP2837400B2 - Plant growth regulator and method for enhancing stress resistance of plant - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a plant growth comprising a plant containing at least one heterocyclic compound or a salt thereof, which is selected as an indicator that the plant has an effect of enhancing environmental stress resistance. In cultivating plants, by applying the plant growth regulator to a plant or soil, etc., the plant induces or stimulates an environmental stress tolerance mechanism possessed by the plant and enhances the plant's resistance to environmental stress. The present invention relates to a method for stabilizing agricultural and horticultural crop cultivation and improving productivity.

[0002]

2. Description of the Related Art It is an important issue in agricultural production to promote the growth of crops and increase the yield per unit area to increase the yield. It is being used for development. Plant growth regulators, such as gibberellins and auxins, are used for controlling germination, rooting, elongation, flowering, fruit growth, and other morphogenetic reactions. It is multifaceted and complex, with limited applications. On the other hand, instead of directly controlling the growth and morphogenesis of plants, substances for stabilizing cultivation and increasing yield by increasing the resistance of plants to environmental stresses such as temperature, moisture, oxygen, etc. Is also being developed. As such substances, vitamin B ₁ , biotin, abscisic acid, benzyladenine and the like, as well as a part of dwarfing agents such as chlormequat, are known. However, development of a plant growth regulator which is more excellent in terms of effectiveness and economy and a method for enhancing environmental stress resistance of a plant using the same have been desired.

[0003]

According to the present invention, there is provided a pyridine-2-carboxylic acid, pyridine-2,3-dicarboxylic acid, pyridine-2,4-dicarboxylic acid, pyridine-2,5-dicarboxylic acid. Acid, pyridine-2,6-dicarboxylic acid, 4-amino-3,5,6-trichloropyridine-2-carboxylic acid, 3-hydroxypyridine-2-carboxylic acid, 5-butylpyridine-2-carboxylic acid, -Substituted or unsubstituted pyridine-2-carboxylic acid selected from the group consisting of -hydroxypyridine-2,6-dicarboxylic acid and 5-hydroxypyridine-2-carboxylic acid or at least one salt thereof as an active ingredient. A plant growth regulator having an action of enhancing plant stress resistance. The present invention according to claim 2 is a method for enhancing stress resistance of a plant, which comprises applying the plant growth regulator according to claim 1 when cultivating the plant.

[0004]

BEST MODE FOR CARRYING OUT THE INVENTION The substituted heterocyclic compound related to the plant growth regulator of the present invention is the above-mentioned substituted or unsubstituted pyridine-2-carboxylic acid or a salt thereof. Plants suitable for the present invention include vegetables such as radish, trefoil, komatsuna, lettuce, spinach, radish, potato; and crops such as rice, wheat, corn, etc., as well as agricultural and horticultural crops such as flowers and fruit trees. .

[0005] The substituted heterocyclic compound of the present invention may be used as it is or diluted with a liquid carrier such as water; a solid carrier such as talc or clay; or other suitable carrier. An auxiliary agent such as an agent can be added for use.

[0006] The plant growth regulator of the present invention is prepared by mixing the substituted heterocyclic compound of the present invention with various liquid carriers or solid carriers by a method generally adopted in the production of agricultural chemicals, and if necessary, wet-wetting. Additives such as agents, spreading agents, dispersants, lubricants, fixatives, and surfactants are added to make the formulation into wettable powders, liquids, emulsions, powders, granules, and the like.

Although the plant growth regulator of the present invention may be used alone, kasugamycin, validamycin, blasticidin S, polyoxin, fusalide, probenazole, hydroxyisoxazole, pyroquilon, tricyclazole, benomyl, thiophanate methyl, thiabendazole , Thiuram, isoprothiolane, mepronil, flutranyl, pencyclone, IBP, EDDP,
The object of the present invention can be achieved more effectively and labor-saving when used in combination with a pesticide such as an EBI agent and / or a pesticide such as an insecticide or a fertilizer.

[0008] The plant growth regulator of the present invention can be used by directly spraying it on a crop where enhancement of resistance to environmental stress is desired. Further, if necessary, the present invention can be applied to a plant growth environment such as a water surface or a soil surface, and can be used by being mixed with soil. Furthermore, it can also be used by attaching it to seeds.

When the plant growth regulator of the present invention is used as a liquid, it is usually desirable that the substituted heterocyclic compound of the present invention is contained in the spray liquid at a concentration of 0.01 to 1000 ppm. When used as powders, granules and the like, it is desirable that the content is 0.1 to 30%. In addition, when it is attached to the seed, it is desirable to attach it at a rate of 0.1 to 100 μg per seed. The application rate can be changed depending on the purpose of use, the method of use or the size of the target crop, the cultivation density, the degree of environmental stress, etc.Examples of the use in paddy fields to reduce low-temperature damage to rice are given. For example, if it is a wettable powder (active ingredient 40%) per 10 ares, 1000 to 10,000
25-250 liters of the doubled solution, granules (active ingredient 1
%), And 1 to 10 kg for powder (1% of active ingredient) is generally appropriate.

As a result, even under environmental stress conditions such as low temperature and low oxygen, for example, root elongation becomes active, and cultivation can be stabilized, yield can be improved, and quality can be improved.

[0011]

Next, the present invention will be described in more detail with reference to examples. However, the present invention is not limited to the following examples, and employs various deformation or modification means within the scope of the claims. It goes without saying that it can be done.

Example 1 (Hypoxic stress) The effect of various heterocyclic compounds on hypoxic stress was examined using barley radicle elongation activity as an index. After germination treatment, the barley seeds were sown on 0.8% agar containing a hydroponic solution and a test solution, and cultivated at 25 ° C. for 12 hours under lighting conditions for 4 days. Thereafter, the roots of each solid were extracted from the agar and the lengths of the longest roots were compared. In the cultivation system used here,
It was confirmed that the dissolved oxygen in the rhizosphere was less than 0.5 ppm, indicating that the barley roots grew under low oxygen stress conditions. Table 1 shows the types of substituted heterocyclic compounds used as test liquids together with the results. In addition, the numerical value in a table | surface shows the expansion | extension ratio of the root of the said substitution heterocyclic compound addition area | region with respect to the elongation of the root of an object area | region as a control as 100. ()
Is the optimal concentration.

[0013]

[Table 1] Table 1 Barley radicle elongation activity ──────────────────────────────────── Compound name Organism Activity% (optimum concentration ppm) ピ リ ジ ン Pyridine-2-carboxylic acid 170 (10) pyridine-2,3-dicarboxylic acid 122 (10) pyridine-2,4-dicarboxylic acid 120 (0.01) pyridine-2,5-dicarboxylic acid 124 (10) pyridine-2,6-dicarboxylic acid 135 (1) 4-amino-3,5,6-117 (0.01) trichloropyridine-2-carboxylic acid 3-hydroxypyridine-2-carboxylic acid 133 (10) 5-butylpyridine-2-carboxylic acid 209 (1) 4-hydroxypyridine-2,6-dicarboxylic acid 1 44 (10) 5-hydroxypyridine-2-carboxylic acid 200 (10) ───────────────────────────────── ───

Example 2 (Low-temperature stress) The low-temperature stress-reducing effect of 5-hydroxypyridine-2-carboxylic acid was examined using the radicle elongation activity of rice as an index. Rice seeds (Nipponbare) were sterilized with 0.5% sodium hypochlorite, and then germinated at 32 ° C. for 2 days. The test solution was sterilized by filtration, placed in a cultivator having a combination of a test tube and a stainless steel mesh for 8 minutes, germinated seeds were set, and lit under a condition of 5000 lux, 12 hours of illumination and 17 ° C. for 5 minutes.
Cultivated for days. The results are shown in Table 2. The numerical values in the table show the ratio of the root elongation in the group to which the substituted heterocyclic compound was added to the root elongation in the control group as 100 as a control. () Is the optimum concentration.

[0015]

[Table 2] Table 2 Rice radicle elongation activity ────────────────────────────────── Compound name Biological activity% (Optimal concentration ppm) ５− 5-hydroxypyridine-2-carboxylic acid 155 (1) ──────────────────────────────────

Example 3 (Salt stress) The effect of 5-butylpyridine-2-carboxylic acid and 5-hydroxypyridine-2-carboxylic acid on reducing salt stress was examined using the radicle elongation activity of tomato as an index. 25 tomato seeds (Chelsea mini) on wet filter paper
After germination treatment in the dark at 2 ° C. for 2 days, various test solutions containing 3000 ppm of sodium chloride were further cultivated on filter paper impregnated with the test solution for 2 days. A concentration of 3000 ppm of sodium chloride is a concentration that halves elongation of tomato radicles.
Table 3 shows the results. The numerical values in the table show the ratio of the root elongation in the group to which the substituted heterocyclic compound was added to the root elongation in the control group as 100 as a control. () Is the optimum concentration.

[0017]

[Table 3] Tomato radicle elongation activity 化合物 Compound name Biological activity% (Optimal concentration ppm) ────────────────────────────────── 5-Butylpyridine-2-carboxylic acid 117 (10) 5-hydroxypyridine-2-carboxylic acid 123 (10)

Example 4 (Low-Temperature Stress) In Example 2, 5-hydroxypyridine-2-carboxylic acid having a low-temperature stress-reducing effect was applied at the time of planting cucumber seedlings. One cucumber seedling
Normally cultivated in No. 3.5 pot for months, before the planting, to prepare a test solution of each concentration using the wettable powder described in Example 7 below,
This was irrigated (20 ml / pot). The seedlings were planted in a 1 / 5000a Wagner pot set at a soil temperature of 16 ° C. Thereafter, the ground temperature was 16 ° C, and the room temperature was 24/16 ° C (day /
Night) for 20 days, and the tension of the roots was compared by dry matter weight. Table 4 shows the results. () Is the index for the control. It was shown as the average value of 10 individuals in each section. As is clear from the table, the effect of encouraging root growth was recognized.

[0019]

[Table 4] Table 4 Results of low-temperature cucumber cultivation ────────────────────────── Concentration ppm Root dry weight mg / pot ───── ０ 0 318 (100) 50 330 (104) 100 335 (105) 500 385 (121) 1000 410 (129) 2000 343 (109) ) ──────────────────────────

Example 5 (Low-temperature stress) In Example 2, 5-hydroxypyridine-2-carboxylic acid, which was confirmed to have a low-temperature stress reducing effect, was used for cultivation for raising rice seedlings. The growth was examined. Example 6 described below in commercially available paddy rice cultivation soil
Were mixed at various concentrations to prepare samples. Nipponbare germinated seeds were sown on each prepared soil, cultivated for a total of 20 days after emergence, greening, and hardening, and root growth was compared by root length and root dry weight. Cultivation conditions: emergence: dark place 32 ° C, 48 hours, greening: 10klux 12 hours lighting, 25/20 ° C (day / night) 48 hours, curing: 35klux 12 hours lighting, 20
/ 18 ° C (day / night). Table 5 shows the results. () Is the index for the control. The average value was shown for 20 individuals in each section. As is clear from the table, an effect was observed on root growth.

[0021]

[Table 5] Table 5 Results of rice seedlings at low temperature ────────────────────────────── Concentration ppm Longest root length mm Root dry weight mg / individual ────────────────────────────── 0 33.1 (100) 4.2 (100) 1 33.8 (102) 4.3 (102) 5 43.3 (131) 5.1 (121) 10 39.7 (120) 4.8 (115) 100 33.2 (100) 4.3 (102) ── ────────────────────────────

Example 6 (Powder) (parts by weight) 5-Hydroxypyridine-2-carboxylic acid 1 Clay 62 Talc 36 Calcium stearate 1 A powder containing 1% of an active ingredient is obtained by uniformly pulverizing and mixing the above component substances. Was.

Example 7 (Wettable powder) (parts by weight) 5-Hydroxypyridine-2-carboxylic acid 20 Lactose 80 The above component substances were uniformly ground and mixed to obtain a wettable powder containing 20% of the active ingredient. .

[0024]

Industrial Applicability The present invention provides a plant growth regulator comprising at least one heterocyclic compound or a salt thereof selected as an index for enhancing environmental stress resistance to plants, and a plant growth regulator comprising the compound. The present invention relates to a method for enhancing the environmental stress resistance of a plant using the method, such as enhancing the adaptability of the plant to environmental stresses such as low temperature, wet damage, and water stress, and promoting root growth under environmental stress conditions. The effect is extremely effective in stabilizing production of agricultural and horticultural crops, improving productivity, improving quality, and the like.

──────────────────────────────────────────────────続 き Continuing from the front page (72) Kunitaka Tachibana, Inventor 760 Meijiokacho, Kohoku-ku, Yokohama, Kanagawa Prefecture Inside the Pharmaceutical Research Institute (72) Inventor Takashi Adachi 5-3-1 Chiyoda, Sakado, Saitama (58) Investigated field (Int. Cl. ⁶ , DB name) A01N 43/40 101 CA (STN) REGISTRY (STN)

Claims (2)

(57) [Claims] 1. Pyridine-2-carboxylic acid, pyridine-
2,3-dicarboxylic acid, pyridine-2,4-dicarboxylic acid, pyridine-2,5-dicarboxylic acid, pyridine-2,
6-dicarboxylic acid, 4-amino-3,5,6-trichloropyridine-2-carboxylic acid, 3-hydroxypyridine-2-carboxylic acid, 5-butylpyridine-2-carboxylic acid, 4-hydroxypyridine-2, Plant stress resistance, comprising as an active ingredient at least one of substituted or unsubstituted pyridine-2-carboxylic acid selected from the group consisting of 6-dicarboxylic acid and 5-hydroxypyridine-2-carboxylic acid, or a salt thereof. A plant growth regulator characterized by having an action of enhancing the growth of a plant. 2. A method for enhancing stress resistance of a plant, which comprises applying the plant growth regulator according to claim 1 when cultivating the plant.