JP4954771B2 - Method for producing low protein rice using white rice as treated rice - Google Patents

Description

  The present invention relates to a method for producing low protein rice using white rice as treated rice.

Low protein rice is useful for people who have restricted protein intake and those who develop food allergies. As a method for producing such low protein rice, “allergens can be reduced. A patent application has already been filed under the name of “december and its production method” (see Patent Document 1). One embodiment of the production method is a method of reducing the protein contained in the rice by treating the rice with a proteolytic enzyme to decompose the protein contained in the rice.
Japanese Patent Publication No. 6-9472

As a result of intensive studies on the above-mentioned known “method for producing cereals with reduced allergens” by carrying out a method for producing low protein rice using white rice as a treated rice, the present proteolytic enzyme is found in white rice In order to reach the protein of the rice, it is necessary to pass through the tissue and cell wall of rice, such that these tissues and cell walls delay the arrival of the proteolytic enzyme to the center of the white rice, and the proteolytic enzyme white rice It was found that protein degradation efficiency was poor due to the delay in reaching the center.

  The present invention has been made on the basis of such findings. The main object of the present invention is to effectively use a known plant tissue disrupting enzyme to promote the arrival of proteolytic enzymes to the center of white rice. Thus, it is to improve the protein degradation efficiency.

The present invention relates to a method for producing low protein rice using white rice as treated rice. The method for producing low protein rice according to the present invention is a method for producing low protein rice in which white rice is treated with a proteolytic enzyme to degrade the protein contained in white rice to reduce the protein. prior to processing the polished rice with proteolytic enzymes, it is also of that processes in plant tissue-disruptive enzymes.

Thus, in the method for producing low protein rice according to the present invention, the treatment of white rice with plant tissue-disrupting enzyme is weakly generated in the anode chamber by diaphragm electrolysis using tap water or dilute saline as electrolyzed water. Acid electrolysis is carried out in acidic water, and the treatment with white rice proteolytic enzyme is carried out in weakly alkaline electrogenerated alkaline water generated in the cathode chamber by diaphragm membrane electrolysis using tap water or dilute saline as electrolyzed water. It is characterized by this. In the method for producing low protein rice according to the present invention, it is preferable to employ pectinase as the plant tissue-disintegrating enzyme and protease as the proteolytic enzyme.

  In the method for producing low protein rice according to the present invention, prior to the treatment with white rice proteolytic enzyme, the rice to be treated is treated with a plant tissue-disintegrating enzyme, and the treatment with the plant tissue-disintegrating enzyme, It destroys in advance the tissues and cell walls that are obstacles for the proteolytic enzymes to invade into white rice. For this reason, in the subsequent treatment of white rice with proteolytic enzymes, it becomes easier for the proteolytic enzymes to penetrate into the white rice, and the protein quickly reaches the center of the white rice. The protein degradation efficiency can be greatly improved.

In particular, in the method for producing low protein rice according to the present invention, the treatment with white rice plant tissue-disintegrating enzyme is performed in the anode chamber by diaphragm membrane electrolysis using tap water or dilute saline as electrolyzed water. In weakly alkaline electrolytically generated alkaline water produced in the cathode chamber by diaphragm membrane electrolysis using tap water or dilute saline as the electrolyzed water. Is going. For this reason, in the treatment of white rice with plant tissue-disrupting enzymes, the electrolyzed acidic water weakens the tissues and cell walls of rice, assists the destruction of tissues and cell walls with plant tissue-disrupting enzymes, and proteolytic enzymes are contained in white rice. It will expedite the destruction of the tissues and cell walls that are the obstacles to invade . In addition, the proteolytic enzyme is activated in the electrolytically generated alkaline water to accelerate the penetration into the white rice and the degradation of the protein in the white rice, so that the degradation efficiency of the protein in the white rice can be further improved.

  The present invention is a method for producing low-protein rice using white rice as treated rice. The method for producing low protein rice according to the present invention is a method for producing low protein rice in which white rice is treated with a proteolytic enzyme to degrade the protein contained in white rice to reduce the protein. Prior to treating white rice with a proteolytic enzyme, it is treated with a plant tissue disrupting enzyme. The production method according to the present invention is intended to improve the protein degradation efficiency in white rice and produce the intended low protein rice without requiring a long time. It is a useful rice for those who are restricted or who develop food allergies.

  In the method for producing low protein rice according to the present invention, prior to the treatment of white rice with a proteolytic enzyme, the white rice that is to be treated is treated with a plant tissue-disintegrating enzyme. As the plant tissue-disrupting enzyme for treating white rice, various known plant tissue-disrupting enzymes such as pectinase, cellulase, hemicellulase and the like can be appropriately selected and employed. Similarly, as a proteolytic enzyme for treating white rice, various known proteolytic enzymes such as protease, papain, α-chymotrypsin, trypsin, pepsin and pancreatin can be appropriately selected and employed. A suitable plant tissue disrupting enzyme can include pectinase, and a suitable proteolytic enzyme can include a protease.

The treatment with the plant tissue-disintegrating enzyme of white rice is performed in electrolytically generated acidic water, and the treatment of white rice with a proteolytic enzyme is performed in electrolytically generated alkaline water. In the treatment of the electrolyzed water by each enzyme, the electrolyzed water is weakly electrolyzed acidic water generated by diaphragm electrolysis using tap water or dilute saline as electrolyzed water, or the diaphragm Weakly alkaline electrolyzed alkaline water produced by electrolysis can be suitably employed.

  Compared to the case of using purified water, the treatment with white rice plant tissue-disrupting enzyme that uses electrolytically generated acidic water is a more impediment to the destruction of tissues and cell walls, and this impedes the entry of proteolytic enzymes into white rice. It has the effect of accelerating the collapse of tissues and cell walls. In addition, in the treatment with white rice proteolytic enzyme that uses electrolytically generated alkaline water, the proteolytic enzyme is activated and penetrates into white rice and degrades protein in white rice compared to the case of using purified water. It has the effect of improving the protein decomposition efficiency in white rice as soon as possible.

In the present Example, the experiment which performs the process by the plant tissue disintegration enzyme of the white rice which is a to-be-processed rice in purified water, and performs the process by the proteolytic enzyme of white rice in the purified water was tried. In this example, maceroteam A (mainly pectinase derived from Rhizopus sp .: made by Yakult Yakuhin Kogyo Co., Ltd.) is used as a plant tissue disrupting enzyme, and actinase (protease derived from Streptomyces griseus: made by Kaken Pharmaceutical Co., Ltd.) as a proteolytic enzyme. ), And each experiment 1 to 3 was performed by the following method for treatment of white rice with a plant tissue-disintegrating enzyme and treatment of white rice with a proteolytic enzyme. However, this example corresponds to a comparative example with respect to Example 2 described later in which the treatment with each enzyme is performed in electrolyzed water.

  (Experiment 1): 20 g of white rice, which is the treated rice, was washed with 28 mL of purified water, and 40 mg of Macero Team A and 28 mL of purified water were added to the washed white rice and reacted at 50 ° C. for 2 hours. Thereafter, the enzyme solution was discarded, and 80 mg of actinase and 28 mL of purified water were added and reacted at 50 ° C. for 22 hours. The enzyme-treated white rice was washed with stirring with 100 mL of purified water for 1 hour.

  (Experiment 2): 20 g of white rice, which is to be treated, was washed with 28 mL of purified water, and 40 mg of maceroteam A, 80 mg of actinase, and 28 mL of purified water were added to the washed white rice and reacted at 50 ° C. for 24 hours. The enzyme-treated white rice was washed with stirring with 100 mL of purified water for 1 hour.

  (Experiment 3): 20 g of white rice as the treated rice was washed with 28 mL of purified water, 80 mg of actinase and 28 mL of purified water were added to the washed rice and reacted at 50 ° C. for 24 hours. The enzyme-treated white rice was washed with stirring with 100 mL of purified water for 1 hour.

  In each of these experiments, the amount of protein and free amino acid in the rice washing solution after washing, the treatment solution after treatment with each enzyme, and the washing solution after stirring and washing was measured, and the free amino acid was converted to protein (free amino acid amount / 128 × 110) and added as a protein amount to obtain an analytical value (protein degradation amount). The analysis values obtained are shown in Table 1.

  Referring to Table 1, when the white rice enzyme treatment is the same time, the amount of protein degradation is highest when the white rice is treated with maceroteam A (experiment 1) prior to the treatment with actinase. In the case where white rice was treated with maceroteam A and actinase at the same time (Experiment 2), and when white rice was treated with only actinase (Experiment 3), the amount of protein degradation was almost the same. The results are recognized as teaching that treating white rice with maceroteam A prior to treating with actinase is a very effective method for producing low protein rice.

  In this example, unlike Example 1, an experiment in which white rice, which is a treated rice, is treated with plant tissue-disintegrating enzyme in electrolytically generated acidic water, and white rice is treated with proteolytic enzyme in electrolytically generated alkaline water. Tried. In this example, maceroteam A (mainly pectinase derived from Rhizopus sp .: made by Yakult Yakuhin Kogyo Co., Ltd.) is used as a plant tissue disrupting enzyme, and actinase (protease derived from Streptomyces griseus: made by Kaken Pharmaceutical Co., Ltd.) as a proteolytic enzyme. ), Each experiment 1 to 3 was tried by the following method for treatment of white rice with plant tissue-disrupting enzyme and treatment of white rice with proteolytic enzyme.

  However, in each experiment, the electrolytically generated water employed is electrolytically generated acidic water generated by diaphragm membrane electrolysis using dilute saline as electrolyzed generated water, and electrolytically generated alkaline water. Electrolytically produced acidic water has a pH of 4 and a redox potential of 653 mV, and has characteristics of having a small amount of cations and a large amount of anions. Electrolytically generated alkaline water has a pH of 10 and an oxidation-reduction potential of −626 mV, and has characteristics of having a large amount of cations and a small amount of anions.

  (Experiment 1): 20 g of white rice, which is to be treated, was washed with 28 mL of electrolytically generated acidic water, and then 40 mg of maceroteam A and 28 mL of electrolytically generated acidic water were added to the washed white rice and reacted at 50 ° C. for 2 hours. Thereafter, the enzyme solution was discarded, and 80 mg of actinase and 28 mL of electrolytically generated alkaline water were added and reacted at 50 ° C. for 22 hours. The enzyme-treated white rice was washed with stirring with 100 mL of purified water for 1 hour.

  (Experiment 2): 20 g of white rice, which is to be treated, was washed with 28 mL of electrogenerated alkaline water, and 40 mg of maceroteam A, 80 mg of actinase and 28 mL of electrogenerated alkaline water were added to the washed white rice and reacted at 50 ° C. for 24 hours. . The enzyme-treated white rice was stirred and washed with 100 mL of electrolytically generated alkaline water for 1 hour.

  (Experiment 3): 20 g of white rice as the treated rice was washed with 28 mL of electrolytically generated alkaline water, 80 mg of actinase and 28 mL of electrolytically generated alkaline water were added to the washed white rice and reacted at 50 ° C. for 24 hours. The enzyme-treated white rice was stirred and washed with 100 mL of electrolytically generated alkaline water for 1 hour.

  In each of these experiments, the amount of protein and free amino acid in the rice washing solution after washing, the treatment solution after treatment with each enzyme, and the washing solution after stirring and washing was measured, and the free amino acid was converted to protein (free amino acid amount / 128 × 110) and added as a protein amount to obtain an analytical value (protein degradation amount). The analysis values obtained are shown in Table 1.

  Referring to Table 2, when the enzyme treatment of white rice is the same time, the amount of protein degradation is highest when white rice is treated with maceroteam A prior to treatment with actinase (Experiment 1). In the case where white rice was treated with maceroteam A and actinase at the same time (Experiment 2), and when white rice was treated with only actinase (Experiment 3), the amount of protein degradation was almost the same. This result is recognized as teaching that treating white rice with maceroteam A prior to treating with actinase is a very effective method for producing low protein rice.

  In addition, the experimental results have the same tendency as the experiments 1 to 3 of the first embodiment, but the results of the first to third experiments of the present embodiment are more than the results of the first to third experiments of the first embodiment. It was found that the amount of protein degradation was large. This result is recognized as teaching that when white rice is treated with actinase, the electrolysis-generated alkaline water is more effective than the pure water. Further, it is recognized that the treatment with maceroteam A, which is a pretreatment of the treated rice, teaches that the treatment in electrolytically generated acidic water is more effective than the treatment in purified water.

Claims (2)

White rice and a method of manufacturing a low-protein rice white rice treated with proteolytic enzymes to reduce the protein to degrade proteins containing, the manufacturing how, prior to processing the polished rice with proteolytic enzymes, Treated with plant tissue-disrupting enzyme, the treatment of white rice with the plant tissue-disintegrating enzyme is a weakly acidic electrogenerated acid produced in the anode chamber by diaphragm membrane electrolysis using tap water or dilute saline as electrolyzed water The treatment with white rice is carried out in water, and the treatment with the proteolytic enzyme is performed in weakly alkaline electrogenerated alkaline water generated in the cathode chamber by diaphragm membrane electrolysis using tap water or dilute saline as electrolyzed water. A method for producing low protein rice using white rice to be treated. The method for producing low protein rice according to claim 1, wherein pectinase is employed as the plant tissue-disintegrating enzyme, and protease is employed as the proteolytic enzyme . Production method.