JP4872725B2 - Blood component distribution device and blood component distribution method - Google Patents

Description

  The present invention relates to a blood component distribution apparatus and a blood component distribution method for collecting only a serum portion in a component storage portion in serum and blood cell portions separated in a blood reservoir.

  Currently, in the field of regenerative medicine, research is being carried out to promote regeneration of a subject's tissue by transplanting the stem cell collected from the subject to the subject after being expanded or differentiated outside the body. Stem cells have attracted attention as cells that have the pluripotency to differentiate into various tissues and organs and are the key to regenerative medicine.

  In vitro growth of stem cells is known to be effective if serum is added to the medium, but for the purpose of human treatment, use serum derived from animals other than humans. This should be avoided for safety reasons, and it is required to use serum prepared from blood derived from humans, particularly from subjects. Compared with blood tests, relatively more serum is required for stem cell culture in the field of regenerative medicine.

As a blood component separation device that separates and collects a predetermined component from blood components, for example, a separator that holds a centrifuged blood bag to push out the contents in the blood bag and communicates with the blood bag via a tube An apparatus for moving plasma into a bag is disclosed (see Patent Document 1). In addition, the leukocyte bag after the blood component inside is centrifuged is hung on the hanger of the fixing plate, the handle of the device is operated to remove it from the hook, and the leukocyte bag is pressed against the fixing plate by the press plate, A component having a low specific gravity (plasma component) is transferred to the plasma bag via the connecting tube. And the apparatus which obstruct | occludes a connection tube when the space | interval between a fixed board and a pressing board becomes a predetermined space | interval is disclosed (refer patent document 2).
Japanese Patent Publication No.55-17585 JP 2002-143295 A

  However, in the above-described apparatus, a predetermined blood component is moved from one bag storing centrifuge blood to one sorting bag. For this reason, only one bag in which a predetermined blood component is stored can be obtained by one operation, and the operation efficiency is not good.

  Here, in order to improve the working efficiency, a blood bag as a blood reservoir that stores blood separated for each component is pressed, for example, a serum component is pushed out of the blood bag, and a plurality of component storage units are formed. It is conceivable to move to a separate bag. However, there are differences in the amount of serum components collected in each sort bag due to various factors, for example, the position of each sort bag and the difference in the length of each connecting tube connected to each sort bag. May occur. For this reason, it is necessary to divide serum components evenly into a plurality of sorting bags.

  As one method for equalizing the amount of serum in a plurality of sorting bags, it is conceivable to subdivide while measuring the weight of each sorting bag. However, there are individual differences in the amount of serum, and it is difficult to measure the weight of serum in the blood collection bag before dividing it into blood collection bags. For this reason, it is difficult to equalize the amount of serum in a plurality of sorting bags.

  Furthermore, when the serum is collected in the sorting bag, the air that was in the blood collection bag and the connecting tube flows into the sorting bag, and in the sorting bag together with the air that has been in the sorting bag before the collection of the serum. Air accumulates in the air. It is preferable that this air is not present in order to stabilize the amount of serum in the blood collection bag. Therefore, it is necessary to extract air from the blood collection bag. Conventionally, in order to evacuate the air in the connecting tube and the sorting bag, an operation of holding the sorting bag with each hand has been performed. For this reason, it was difficult to remove air efficiently. Further, Patent Document 1 discloses returning air in a sorting bag to a blood collection bag. However, in this case, it is effective when one collection bag is connected to one blood collection bag, but in the case where a plurality of collection bags are connected to one blood collection bag, The air may flow into other sorting bags and is not effective.

  The present invention has been made in view of the problems as described above, and blood that realizes subdividing predetermined components in the blood reservoir in equal amounts into a plurality of component storage portions so that air does not remain inside. An object is to provide a component distribution device and a blood component distribution method.

  Here, “serum” refers to a pale yellow liquid whose fluidity decreases when the collected blood is allowed to stand and then separated from the red clot (blood clot).

  In order to achieve the above object, the present invention provides the following.

  (1) A blood reservoir that stores a plurality of blood components therein, a connection pipe that is connected to the blood reservoir and sends out a predetermined blood component to the outside of the blood reservoir, a plurality of branch pipes that branch from the connection pipe, and A component separation device having a plurality of component storage units connected to the plurality of branch pipes, and a separation unit that applies pressure to the blood storage unit to move blood components in the blood storage unit to the plurality of component storage units A pressure device, wherein the plurality of component storage units are positioned below the blood storage unit, and a pressure generated by a pressure applied to the blood storage unit and a drop between the blood storage unit and the plurality of component storage units By moving the blood component from the blood separated for each component in the blood storage part to the plurality of component storage parts via the connection pipe and the branch pipe, the blood storage part A blood component distribution device that collects blood components and subdivides the blood components into the plurality of component storage units, wherein the component separation device is connected to a branch pipe branched from the connection pipe, and inflow and outflow of blood components And the branch pipe is set to a length that allows the adjustment reservoir to be positioned above and below the plurality of component storage units, A pressurizing means for applying pressure to the blood reservoir, a component container support means disposed below the blood reservoir and supporting the plurality of component containers, and the adjustment above the plurality of component containers An adjustment storage portion support means for detachably supporting the storage portion for attachment and detachment, wherein the component storage portion support means faces the connecting portion between the branch pipe and each of the component storage portions upward. A fixing plate for installing the housing portion; A movable plate disposed opposite to the fixed plate and opening and closing a plate surface of the fixed plate, and a biasing means for biasing the movable plate toward the fixed plate, the movable plate being A blood component distributor that simultaneously pressurizes the plurality of component storage portions when the plate surface is closed.

  According to the invention of (1), when the predetermined components collected from the blood flow into the plurality of component storage portions and the respective component storage portions swell, the plurality of component storage portions are formed by the fixed plate and the movable plate. Both side portions in the thickness direction are regulated. For this reason, the storable capacity in each component storage unit is kept constant. In addition, for example, when the amount of stored blood components in one component storage portion increases, the movable plate moves against the biasing force of the biasing means. At that time, the pressure in the component storage portion is reduced. growing. For this reason, the amount of blood component flowing into one component storage portion is reduced, and the blood component flows into the component storage portion having a lower pressure. As a result, blood components can be subdivided into equal amounts in a plurality of component storage portions.

  Moreover, since the connection part of a connection pipe and a component accommodating part is orient | assigned upwards, air comes to accumulate in the upper part of a component accommodating part. At this time, the movable plate is pressed by the pressure from the plurality of component storage parts swollen by the pressure from the blood storage part, but by releasing the pressure from the blood storage part, the plurality of components The accommodating portion is evenly pressed by the urging force of the urging means, and air is returned to the upper side of the component accommodating portion toward the connecting pipe. Then, the air moves to and is stored in the adjustment storage unit. Thus, the residual amount of air in the plurality of component storage portions can be reduced.

  (2) In the invention of (1), the blood reservoir has inserted therein a blood coagulation promoting individual that promotes blood coagulation, and the pressurizing means comprises a fixing plate for installing the blood reservoir. A movable plate disposed opposite to the fixed plate and approaching and separating from the plate surface of the fixed plate, and a biasing means for biasing the movable plate toward the fixed plate, A blood component distribution device in which a concave portion is formed at a portion facing the lower portion of the blood reservoir.

  According to invention of (2), since the recessed part is formed in the fixed plate at the site | part facing the lower part of the blood storage part, a blood storage part is set to a pressurization means, a movable plate is moved, and a blood storage part When the pressure is applied to the blood reservoir, the blood coagulation promoting individual that is sunk in the blood reservoir is positioned in the recess. For this reason, when the blood reservoir is sandwiched between the fixed plate and the movable plate, it is prevented that a large pressure is applied to the blood coagulation promoting individual, so that the blood coagulation promoting individual is destroyed and the blood inside the blood reservoir is It is possible to prevent problems such as the component becoming unusable and the blood reservoir being damaged. Further, when the blood reservoir is sandwiched between the fixed plate and the movable plate, the contents of the blood reservoir can be transferred without remaining.

  (3) In the invention of (1), the blood reservoir has a blood coagulation promoting individual inserted therein to promote blood coagulation, and the pressurizing means includes a fixing plate for installing the blood reservoir. A movable plate disposed opposite to the fixed plate and approaching and separating from a plate surface of the fixed plate, and a biasing means for biasing the movable plate toward the fixed plate. A blood component distribution device in which an elastic member in contact with the blood reservoir is provided on at least one plate surface of the movable plate.

  According to the invention of (3), since the elastic member is provided on one or both of the fixed plate and the movable plate, the blood reservoir is set in the pressurizing means, and the movable plate is moved. When pressure is applied to the blood reservoir, the portion of the blood coagulation promoting individual inside the blood reservoir is brought into contact with the elastic member. For this reason, when the blood reservoir is held between the fixed plate and the movable plate, the region of the blood coagulation promoting individual in the elastic member is elastically deformed, and the pressure applied to the blood coagulation promoting individual is absorbed. This prevents a large pressure from being applied to the blood coagulation promoting individual, so that the blood coagulation promoting individual is destroyed and the blood components inside the blood reservoir become unusable or the blood reservoir is damaged. It becomes possible to prevent problems. Further, when the blood reservoir is sandwiched between the fixed plate and the movable plate, the contents of the blood reservoir can be transferred without remaining.

  (4) A blood component distribution method using the blood component distribution device according to (1), (2) or (3), wherein a branch pipe of the adjustment reservoir is closed, and the blood reservoir is Setting the pressurizing means to pressurize the blood reservoir, moving the blood component in the blood reservoir to the plurality of component reservoirs, and closing the connecting pipe connected to the blood reservoir. The adjustment reservoir is positioned below the plurality of component storage units, the branch pipe of the adjustment storage unit is opened, and the plurality of blood components stored in the plurality of component storage units together with the plurality of blood components. The step of moving the air in the component storage unit and the air in the flow path from the plurality of component storage units to the adjustment storage unit to the adjustment storage unit, and closing the branch pipe of the adjustment storage unit, The connecting part between the adjustment reservoir and the branch pipe faces downward. In this state, the adjustment reservoir is positioned above the plurality of component reservoirs, the branch pipe of the adjustment reservoir is opened, and the blood components in the adjustment reservoir are returned to the plurality of component reservoirs. A blood component dispensing method comprising: a step; and a step of sealing the plurality of component accommodating portions and taking out the component accommodating portion supporting means.

  According to the invention of (4), the branching pipe of the adjustment reservoir is first closed, the blood reservoir is set on the pressurizer and the blood reservoir is pressurized. The blood component in the blood reservoir can be moved to a plurality of component reservoirs by the pressure applied to the reservoir and the drop pressure due to the drop between the blood reservoir and the component reservoir. Next, the adjustment reservoir is positioned below the plurality of component storage units, and the branch pipe of the adjustment storage unit is opened, so that the pressure applied to the component storage unit by the component storage unit support means and the adjustment storage unit The air in the plurality of component storage units and the air in the flow path from the component storage unit to the adjustment storage unit together with some of the blood components stored in the plurality of component storage units due to the drop pressure due to the drop between the component storage unit and the component storage unit Can be moved to the adjustment reservoir and stored in the adjustment reservoir.

  Next, the adjustment reservoir is positioned above the plurality of component storage units, and the adjustment pipe is opened by opening the branch pipe of the adjustment storage unit, thereby reducing the adjustment pressure due to the drop pressure between the adjustment storage unit and the component storage unit. Only the blood component can be returned from the reservoir to the plurality of component storage units. As a result, it is possible to distribute the blood component evenly and efficiently to a plurality of component storage portions with less residual air, and to move the blood components to the plurality of component storage portions without waste. become.

  As described above, according to the present invention, it becomes possible to distribute a predetermined blood component of the blood reservoir to a plurality of component storage units with a small amount of residual air and efficiently distribute the blood component evenly. In addition, it is possible to move blood components to a plurality of component storage units without waste.

Hereinafter, embodiments of the present invention will be described in detail with reference to the drawings.
FIG. 1 is an explanatory diagram showing the configuration of the component distribution apparatus according to the first embodiment of the present invention. The blood component distribution device collects only serum from a blood storage unit that stores blood separated for each component and moves it to a predetermined component storage unit, and stores air in the component storage unit in the adjustment storage unit The blood component separation device 1 is divided into a blood device separation device 1 and a support device 2 that supports the blood component separation device 1. The support device 2 is detachably attachable to the pressurizing device 3 that presses the blood reservoir and moves the internal serum component to the component housing, the component housing support device 4 that supports the component housing, and the adjustment reservoir. It is comprised by the stand 5 to support.

  The blood component separation device 1 includes a blood collection bag 100 as a blood storage part in which blood is stored, a plurality of sorting bags 200 as component storage parts for storing serum collected from the blood collection bag 100, and a part of the serum. The adjustment bag 300 temporarily stores, the connection tubes 400 to 406 connecting the blood collection bag 100, the sorting bag 200, and the adjustment bag 300, and the branch tubes 450 to 452 that branch the flow path from the blood collection bag 100. Has been.

  FIG. 2 is an explanatory view showing the configuration of the pressurizing device, FIG. 2 (a) shows the overall configuration of the pressurizing device, and FIG. 2 (b) is an enlarged view of the main part of FIG. 2 (a). is there. As shown in FIG. 2A, a fixed plate 31 is erected in a vertical direction on a base 30 having a horizontal plane. A movable plate 32 is rotatably provided at a lower portion of the fixed plate 31 via a hinge portion (not shown). A recess 31 a is formed at the center lower portion of the plate surface of the fixed plate 31. The movable plate 32 is formed as a single flat plate by connecting the lower metal plate 32 a and the upper transparent plate 32 b, and the plate surface of the movable plate 32 is substantially the same as the plate surface of the fixed plate 31. It is a plate surface of a size. One end of a lever 33 is attached to the opposite side of the metal plate 32a facing the fixed plate 31. The shaft portion of the lever 33 is provided so as to form an acute angle with respect to the opposite surface of the movable plate 32 to the surface facing the fixed plate 31. Is provided. A hook 35 is erected on the upper portion of the fixed plate 31, and a substantially L-shaped lever hook 36 is rotatably provided on the front portion of the base 30. In addition, the recessed part 31a formed in the fixing plate 31 should just have the depth more than the outer diameter of the glass processing body 105 accommodated in the blood collection bag 100 inside, and may penetrate the plate | board surface.

  2A, the lever 33 is lowered, the movable plate 32 is rotated downward, and engaged with the lever hook 36, whereby the plate surface of the fixed plate 31 is opened. Maintained. Further, by releasing the engagement by the lever hook 36, the movable plate 32 is rotated upward by the urging force of the torsion spring 34 (see FIG. 2B), and the plate surface of the fixed plate 31 and the movable plate 32. The plate surface comes into contact. Here, when the engagement with the lever hook 36 is released, the lever 33 is engaged after the hand is put on the lever 33 so that the plate surface of the movable plate 32 does not collide with the plate surface of the fixed plate 31. It is preferable that the movable plate 32 is rotated upward by operating the lever 33.

  Further, as shown in FIG. 2B, the movable plate 32 is engaged with the fixed plate 31 and a part of the movable plate 32 on both sides at the center lower portion, and the movable plate 32 is always attached to the fixed plate 31. A torsion spring 34 is provided to urge the movable plate 32 in the closing direction, that is, so that the movable plate 32 rotates toward the fixed plate 31. The spring constant of the torsion spring 34 is such that when the lever 33 is lowered and the movable plate 32 is rotated downward, the blood collection bag 100 ( 1), an appropriate pressing force can be applied.

  Although details will be described later, the blood collection bag 100 storing the blood after centrifugation is suspended on the hook 35 of the fixed plate 31 and brought into contact with the plate surface, and is movable together with the lever 33 while maintaining the state. By rotating the plate 32 and closing the movable plate 32, the blood collection bag 100 is sandwiched between the fixed plate 31 and the movable plate 32 with a predetermined pressure applied. Further, by rotating the lever 33 downward, the movable plate 32 is rotated downward in conjunction with the lever 33, the plate surface of the fixed plate 31 is opened, and the blood collection bag 100 can be installed and replaced. .

  FIG. 3 is an explanatory view showing the configuration of the component storage unit support device, FIG. 3 (a) shows the overall configuration of the component storage unit support device, and FIG. 3 (b) shows the main part of FIG. 3 (a). It is an enlarged one. As shown in FIG. 3A, a fixed plate 41 is erected in a vertical direction on a base 40 having a horizontal plane. Under the fixed plate 41, a movable plate 42 is rotatably provided via a hinge portion (not shown). The movable plate 42 is formed by connecting a lower metal plate 42a and an upper transparent plate 42b to form a substantially T-shaped flat plate. One end of a lever 43 is attached to the opposite side of the metal plate 42a facing the fixed plate 41. The shaft portion of the lever 43 is provided so as to form an acute angle with respect to the opposite surface of the movable plate 42 to the surface facing the fixed plate 41, and the other end portion of the lever 43 has a spherical body so that it can be easily operated. Is provided. A substantially L-shaped lever hook 46 is provided on the front surface of the base 40.

  In addition, three sets of hooks 45 are erected on the upper surface of the fixed plate 41, and these hooks 45 are provided in parallel in the horizontal direction. The hook 45 hangs the sorting bag 200. In the present embodiment, the three sorting bags 200 can be set on the fixing plate 41 at the same time. In addition, the size of the plate surface of the transparent plate 42 b is set to a size that can be simultaneously opposed to at least a portion of the bag body in all the sorting bags 200 set on the fixed plate 41.

  3A, the lever 43 is lowered, the movable plate 42 is rotated downward, and the lever 43 is engaged with the lever hook 46, whereby the plate surface of the fixed plate 41 is released. Maintained. Further, by releasing the engagement by the lever hook 46, the movable plate 42 is rotated upward by the urging force of the torsion spring 44 (see FIG. 3B), and the plate surface of the fixed plate 41 and the movable plate 42 are moved. The plate surface comes into contact. Here, when the engagement by the lever hook 46 is released, the lever 43 is engaged after the hand is put on the lever 43 so that the plate surface of the movable plate 42 does not collide with the plate surface of the fixed plate 41. It is desirable that the movable plate 42 is rotated upward by operating the lever 43.

  Further, as shown in FIG. 3B, the movable plate 42 is engaged with the fixed plate 41 and a part of the movable plate 42 on both sides at the lower center of the movable plate 42. A torsion spring 44 is provided to urge the movable plate 42 so that the movable plate 42 rotates toward the fixed plate 41. The spring constant of the torsion spring 44 is the sorting bag 200 that is clamped between the movable plate 42 and the fixed plate 41 by the bias of the torsion spring 44 when the lever 43 is lowered and the movable plate 42 is rotated downward. It is set so that an appropriate pressing force can be applied to. In the present embodiment, in FIG. 1, the pressing force applied to the sorting bag 200 is greater than the sum of the pressing force applied to the blood collection bag 100 by the pressurizing device 3 and the drop pressure from the blood collection bag 100 to the sorting bag 200. Is set to be small.

  FIG. 4 is an explanatory view showing a state in which the sorting bag 200 is set in the storage unit support device. The empty sorting bag 200 is set on the fixed plate 41, and the movable plate 42 is rotated together with the lever 43 to close the movable plate 42, whereby the sorting bag 200 is placed between the fixed plate 41 and the movable plate 42. Be placed. Further, by rotating the lever 43 downward, the movable plate 42 is rotated downward in conjunction with the lever 43 and the plate surface of the fixed plate 41 is opened, so that the sorting bag 200 can be installed and replaced. Become.

  The adjustment bag 300 is detachably supported by the stand 5 (see FIG. 1). That is, when the adjustment bag 300 is attached to the stand 5, the adjustment bag 300 is positioned vertically above the sorting bag 200. Furthermore, the connection tube 405 connected to the adjustment bag 300 is set to a length that allows the adjustment bag 300 to be positioned vertically below the sorting bag 200 when the adjustment bag 300 is removed from the stand 5.

  FIG. 5 is an explanatory diagram showing the configuration of the blood component separation device.

  A connection port 102 is formed at the upper edge of the main body 101 of the blood collection bag 100, and one end of a connection tube 400 is airtightly connected to the connection port 102. In addition, a communication tube 103 for connecting the blood collection bag 100 and the connection tube 400 is provided in the lumen portion of the connection port 102. A branch pipe 450 is connected to the other end of the connection tube 400, and a branch pipe 451 is connected to one branch path of the branch pipe 450 via a connection tube 401, and each branch path of the branch pipe 451 is connected. The sorting bag 200 is hermetically connected via the connecting tubes 402 and 403. A branch pipe 452 is connected to the other branch path of the branch pipe 450 via a connection tube 404, and the adjustment bag 300 is hermetically connected to one branch path of the branch pipe 452 via a connection tube 405. ing. In addition, the sorting bag 200 is hermetically connected to the other branch path of the branch pipe 452 via a connecting tube 406.

The main body 101 of the blood collection bag 100 is constituted by a bag, and each is made of soft polyvinyl chloride or the like. Moreover, the inside of the blood collection bag 100 is sterilized in advance. In addition, holes 110 are formed on both sides of the connection port 102 provided in the upper part of the blood collection bag 100 so as to be loosely fitted to the hook 35 (see FIG. 3). Further, a plurality of glass processed bodies 105 serving as blood coagulation promoting individuals are accommodated in the main body 101. The glass processed body 105 has, for example, a substantially spherical shape made of soda glass. Moreover, in this embodiment, although it is set as the structure provided with the glass processing body 105 in the blood collection bag 100, when obtaining the blood coagulation promotion function, the surface area of the glass processing body 105 with respect to the blood volume which can be stored is 0.1. It is preferable to set with a relationship of (mm ² / ml) or more.

  Note that the glass processed body 105 is not joined to the inner wall of the main body portion 101 or the like, and when the main body portion 101 is given a shaking action or a vibration action, the glass processed body 105 is in a state in which it can float inside the main body portion 101. Is set.

In order to prevent the red blood cells in the blood from being destroyed and causing hemolysis when the body 101 is shaken or vibrated after the blood is stored, or even when the blood is centrifuged, It is preferable to set the glass processed body 105 with a surface area having a relationship of 25.0 (mm ² / ml) or less with respect to the amount of blood that can be stored in the main body 101 of the blood collection bag 100.

  Moreover, the main body parts 201 and 301 of the sorting bag 200 and the adjustment bag 300 are formed of a bag body, and each is made of soft polyvinyl chloride or the like. These are also sterilized in advance. In addition, holes 210 that are loosely fitted to the hooks 45 are formed on both sides of the connection port 205 provided in the upper part of the sorting bag 200. A hole 320 is formed in the upper part of the adjustment bag 300. The hole 320 is used to suspend the hole 320 on a predetermined instrument with the connection port 305 facing up. In addition, a hole 310 for loosely fitting and hanging the adjustment bag 300 on the suspension hanger 5a of the stand 5 is formed at a portion opposite to the connection port 305 of the connection tube 405 in the adjustment bag 300 in the vertical direction. ing.

  Further, the connecting tubes 401 to 406 serve as a lead-out path for leading out the separated blood components. These connection tubes 401 to 406 are made of a flexible resin material, for example, a material such as soft polyvinyl chloride.

  Further, the size of the connecting tube 400 is set such that the inner diameter dimension thereof is smaller than the outer dimension dimension of the glass processed body 105. This is to prevent the glass processed body 105 from entering the connecting tube 400 when preparing and deriving serum.

  Furthermore, as shown in FIG. 1, in the blood component separation apparatus 1, blood and extraction are performed by sandwiching the required portions of the connection tubes 401 to 406, that is, the connection tubes 400, 402, 403, 405, and 406 with clamps 500 to 504. When the obtained serum is derived, the flow path can be switched. In this embodiment, the connection tube 400 is provided with a clamp 500, the connection tube 405 is provided with a clamp 501, and the connection tubes 402, 403, and 406 are provided with clamps 502, 503, and 504.

  Next, the collection | recovery operation | work of the serum component using the apparatus shown in FIG. 1 is demonstrated. First, the blood collection bag 100 storing the collected blood is applied to a centrifuge (not shown) to separate the blood into components. At this time, the serum component forms the uppermost layer in the component layer in the blood collection bag 100. In the blood component separation device 1, the clamp 500 and the clamp 501 are closed in advance, and the clamps 502, 503, and 504 are opened. Furthermore, the support position of the adjustment bag 300 set on the stand 5 is the highest, and then the blood collection bag 100 set on the pressurizing device 3 is high, and the sorting bag set on the component storage unit support device 4. The positions of the pressurizing device 3, the component storage unit supporting device 4, and the stand 5 are set so that the position of 200 becomes the lowest.

  Next, the blood collection bag 100 is set in the pressurizing device 3. First, the lever 33 is lowered and the movable plate 32 is rotated downward to open the plate surface of the fixed plate 31. Next, the connection port 102 is directed upward, and the blood collection bag 100 is brought into contact with the plate surface of the fixed plate 31 with the portion where the glass processed body 105 exists facing the recess 31a, so that the hole 110 of the blood collection bag 100 is formed. The blood collection bag 100 is suspended by loosely fitting the hook 35, and the movable plate 32 is closed by rotating the movable plate 32 together with the lever 33 while maintaining the state. At this time, the urging force of the torsion spring 34 is applied to the blood collection bag 100 via the movable plate 32, so that the blood collection bag 100 is sandwiched between the fixed plate 31 and the movable plate 32 with a predetermined pressure applied. . At the same time, no pressure is applied to the glass processed body 105.

  Next, the sorting bag 200 is set on the component storage unit support device 4. First, the lever 43 is lowered and the movable plate 42 is rotated downward to open the plate surface of the fixed plate 41. Next, the hole 210 of the sorting bag 200 is loosely fitted to the hook 45 and the sorting bag 200 is suspended. By hanging the sorting bag 200 on all three hooks 45, the three sorting bags 200 are set on the fixing plate 41 with the connection port 205 facing upward. At this time, the three sorting bags 200 are positioned vertically below the blood collection bag 100. Then, the movable plate 42 is rotated together with the lever 43 to close the movable plate 42.

  Next, the communication tube 103 is broken, and the blood collection bag 100 and the connection tube 400 are communicated. Further, the clamp 500 is manually opened, and the flow path of the connection tube 400 is opened. At this time, the serum component in the blood collection bag 100 is pushed out by the pressurization by the pressurization device 3, and the serum is put into the three sorting bags 200 via the connecting tubes 400 to 404, 406 as shown by arrows in FIG. The component moves. As a result, serum components are stored in the three sorting bags 200. After the passage of time, as the storage amount increases, the sorting bag 200 expands, and the sorting bag 200 presses the movable plate 42 and slightly rotates.

  Here, when the clamp 500 is opened, as shown by the arrows in FIG. 6, the air remaining in the connection tubes 400 to 404 and 406 is pushed out together with the serum components into the three sorting bags 200 and separated. Accumulated on top of bag 200. When a predetermined amount of serum component is stored in the three sorting bags 200, the clamp 500 is closed and the flow path of the connecting tube 400 is closed.

  Next, after positioning the adjustment bag 300 below the sorting bag 200, the clamp 501 is manually opened, and the flow path of the connection tube 405 is opened. At this time, since the three sorting bags 200 are pressurized by the movable plate 42, as shown by the arrows in FIG. A part moves to the adjustment bag 300 through the connection tubes 401-406. Then, when all of the air moves from the three sorting bags 200 to the adjustment bag 300 and the connection tubes 401 to 406 are filled with serum components, the clamp 501 is closed and the flow path of the connection tube 405 is closed. To do.

  Next, the suspension hanger 5 a is inserted into the hole 310 of the adjustment bag 300, and the adjustment bag 300 is suspended from the stand 5. At this time, since the connection port 305 of the adjustment bag 300 faces downward, in the adjustment bag 300, the vicinity of the connection port 305 is filled with the serum component, and air is stored in the upper portion thereof. In such a state, the clamp 501 is opened and the flow path of the connecting tube 405 is opened, thereby adjusting the pressure by the pressure difference between the adjustment bag 300 and the sorting bag 200 as shown by the arrow in FIG. Serum components in the bag 300 move to the three sorting bags 200. Then, after the serum component is exhausted in the adjustment bag 300, the clamp 502 to 504 is closed to collect the serum component from the blood collection bag 100 and divide it into three sorting bags 200. If it is difficult for the serum component to move from the adjustment bag 300 to the three sorting bags 200, pressure is applied to the adjustment bag 300 by hand to push out the serum components, or the lever 43 is slightly lowered to reduce the three sorting bags. The pressure applied to 200 may be reduced.

  After the separation and collection work is completed, the connection tubes 402, 403, and 406 are melted and the flow path is closed, and then the individual sorting bags 200 are separated from the blood component separation device 1.

  According to the present embodiment configured as described above, the following operational effects can be obtained.

  According to this embodiment, when the serum component flows into the plurality of sorting bags 200 set in the component storage unit support device 4 from the blood collection bag 100 and each sorting bag 200 is inflated, the fixing plate 41 and the movable plate 42 regulate both side portions in the thickness direction of the plurality of sorting bags 200, so that the storable capacity of each sorting bag 200 is kept constant. Further, for example, when the amount of serum component stored in one sorting bag 200 increases, the movable plate 42 moves against the bias of the torsion spring 44. At that time, since the pressure in the sorting bag 200 increases, the inflow amount of blood components decreases, and accordingly, the blood components flow into the sorting bag 200 having a low pressure. As a result, the blood components can be subdivided equally into the plurality of sorting bags 200.

  Moreover, since the connection part of connection tube 402,403,406 and the sorting bag 200 is orient | assigned upwards, air comes to accumulate on the upper part of the sorting bag 200. FIG. At this time, the movable plate is pressed by the pressure from the plurality of sorting bags 200 inflated by the pressure from the blood collection bag 100, but the clamp 501 is closed to release the pressure from the blood collection bag 100. Thus, the plurality of sorting bags 200 are evenly pressed by the urging force of the torsion spring 44, and air is returned to the upper side of the sorting bag 200 toward the connection tubes 402, 403, and 406. Then, the air moves to the adjustment bag 300 and is stored. Thus, the residual amount of air in the plurality of sorting bags 200 can be reduced.

  Moreover, according to this embodiment, since the recessed part 31a is formed in the site | part facing the lower part of the blood collection bag 100 in the stationary plate 31, the blood collection bag 100 is set to the pressurization apparatus 3, and the movable plate 32 is moved. When pressure is applied to the blood collection bag 100, the glass processed body 105 as a blood coagulation promoting individual that is in a sunk state inside the blood collection bag 100 is positioned in the recess 31a. For this reason, when the blood collection bag 100 is held between the fixed plate 31 and the movable plate 32, it is possible to prevent a large pressure from being applied to the glass processed body 105. This makes it possible to prevent problems such as the blood component being unusable or the blood collection bag 100 being damaged. Further, when the blood reservoir is sandwiched between the fixed plate and the movable plate, the contents of the blood reservoir can be transferred without remaining.

  Further, according to the present embodiment, the pressurizing device is configured such that the connecting tube 405 connected to the adjustment bag 300 is closed, the blood collecting bag 100 is set in the pressurizing device 3 and the blood collecting bag 100 is pressurized. 3, the blood component in the blood collection bag 100 can be moved to the plurality of collection bags 200 by the pressure applied to the blood collection bag 100 by 3 and the pressure drop due to the drop between the blood collection bag 100 and the collection bag 200.

  Next, the connection tube 400 connected to the blood collection bag 100 is closed, the adjustment bag 300 is positioned below the plurality of sorting bags 200, and the connection tube 405 connected to the adjustment bag 300 is opened. Thus, a part of blood components stored in the plurality of sorting bags 200 due to the pressure applied to the sorting bag 200 by the component storage unit support device 4 and the drop pressure due to the drop between the adjustment bag 300 and the sorting bag 200. At the same time, the air in the plurality of sorting bags 200 and the air in the flow path from the sorting bag 200 to the adjustment bag 300 can be moved to the adjustment bag 300 and stored in the adjustment bag 300.

  Next, the adjustment bag 300 is positioned above the plurality of sorting bags 200, and the connecting tube 405 connected to the adjustment bag 300 is opened, so that the drop pressure due to the drop between the adjustment bag 300 and the sorting bag 200 is reached. Thus, only the blood component can be returned from the adjustment bag 300 to the plurality of sorting bags 200. As a result, it is possible to distribute the blood components evenly and efficiently to the plurality of sorting bags 200 with less residual amount of air, and to move the blood components to the plurality of sorting bags 200 without waste. Is possible.

  In addition, according to the present embodiment, since pressure is applied to the plurality of sorting bags 200, the blood transfer speed from the blood collection bag 100 to the sorting bag 200 can be kept low, and the blood components of the blood collection bag 100 can be maintained. It is possible to collect serum with reduced contamination of other blood components without disturbing the interface. That is, by applying pressure to the sorting bag 200 so as to maintain the relationship of (pressing force on the blood collection bag 100 + pressure difference)> (pressing force on the sorting bag 200), the serum transfer speed is reduced. It is possible to transfer the serum to the sorting bag 200 while maintaining the same. As a result, serum with less contamination can be transferred to the sorting bag 200.

  FIG. 9 is an explanatory view showing a configuration of a pressurizing apparatus according to the second embodiment of the present invention. In addition, in the member shown in FIG. 9, about the member same as the member of 1st Embodiment shown in FIG. 2, or the member of the same function, the same code | symbol is attached | subjected and detailed description is abbreviate | omitted. The pressurizing device 3 shown in FIG. 9 is a flat plate having a buffering action on the entire surface of the fixing plate 31 as the fixing plate 31 in the pressurizing device 3 shown in FIG. The elastic member 37 is attached. As the material of the elastic member 37, polyurethane, polystyrene, polyolefin resin (polyethylene, polypropylene, etc.), rubber (natural rubber, artificial rubber, silicon rubber), or the like can be used. Examples of the shape include foam (sponge, foam) and gel. Further, as the hardness, when pressed by the glass processed body 105, at least the dimension of the glass processed body 105 needs to be a hardness (low resilience) capable of elastic deformation. Further, the thickness is preferably larger than the size of the glass processed body 105 in order to prevent direct pressure from being applied to the glass processed body 105 from the fixed plate 31 and the movable plate 32 simultaneously. In the pressure device 3 of the second embodiment, a chloroprene rubber sponge is used as the elastic member 37.

  10 is an explanatory view showing a state in which the blood collection bag 100 is pressed by the pressurizing device 3, FIG. 10 (a) is the pressurizing device 3 shown in FIG. 2, and FIG. 10 (b) is the pressurizing device shown in FIG. The case where the pressure apparatus 3 is used is shown. In the pressurizing device 3 shown in FIG. 2, when the movable plate 32 is pressed and rotated toward the fixed plate 31, the glass processed body 105 is positioned in the recess 31 a, so that the glass processing is performed from the fixed plate 31. No pressure is directly applied to the body 105. For this reason, it is prevented that the glass processed body 105 is destroyed. In the pressurizing device 3 shown in FIG. 9, when the movable plate 32 is pressurized and rotated toward the fixed plate 31, the portion of the elastic member 37 facing the glass processed body 105 is elastically deformed, and the glass processing is performed. The pressure from the body 105 is absorbed by the elastic member 37. In addition, no pressure is directly applied from the fixed plate 31 to the glass processed body 105. For this reason, it is prevented that the glass processed body 105 is destroyed.

  According to the second embodiment configured as described above, if the glass processed body 105 exists in the region of the elastic member 37, when the movable plate 32 is pressurized and rotated to the fixed plate 31 side, Since the pressure from the glass processed body 105 is absorbed by the elastic member 37, the glass processed body 105 is prevented from being broken. For this reason, even if the distribution of the plurality of glass processed bodies 105 varies in the blood collection bag 100, the glass processed body 105 can be prevented from being broken.

  Further, since the blood collection bag 100 can be brought into close contact between the elastic member 37 and the movable plate 32, it is possible to press the blood collection bag 105 and the vicinity thereof. For this reason, for example, when taking out the blood cell component in the lowest layer of the separated blood component, it is possible to reduce the residue in the blood collection bag 100.

  In the second embodiment, the elastic member 37 is provided on the fixed plate 31. However, the present invention is not limited to this, and the elastic member 37 may be provided on the movable plate 32. An elastic member 37 may be provided on both of the movable plates 32. Further, in the second embodiment, similarly to the first embodiment, the fixing plate 31 is provided with a recess 31a, or the elastic member 37 is provided with a hole or a slit at a portion opposite to the portion where the glass processed body 105 gathers. Thus, the elasticity may be increased.

  As mentioned above, although embodiment of this invention was described, this invention is not restricted to what was mentioned above. For example, in the above-described embodiment, serum components are collected, but other components in blood components may be extracted. In the above-described embodiment, the three sorting bags 200 are set on the fixing plate 41. However, it is needless to say that the number may be larger than that. The effect is achievable. In the above-described embodiment, the clamp 500 is opened after the movable plate 42 is closed in the component storage unit support device 4. However, the present invention is not limited thereto, and the clamp 500 is opened with the movable plate 42 opened. After closing, the movable plate 42 may be closed, the clamp 501 may be opened, and the air extraction operation may be performed.

It is explanatory drawing which shows the structure of the serum collection system using the component accommodating part support apparatus in 1st Embodiment of this invention. It is explanatory drawing which shows the structure of the pressurization apparatus of FIG. It is explanatory drawing which shows the structure of the component accommodating part support apparatus of FIG. It is explanatory drawing which shows the structure of the blood component separation apparatus of FIG. It is explanatory drawing which shows the state which set the fractionation bag to the component accommodating part support apparatus. It is explanatory drawing which shows the state which has distributed the blood component from the blood collection bag to the fractionation bag. It is explanatory drawing which shows the state which is moving the air in a fractionation bag to an adjustment bag. It is explanatory drawing which shows the state which has distributed the blood component from the adjustment bag to the fractionation bag. It is explanatory drawing which shows the structure of the pressurization apparatus which concerns on 2nd Embodiment of this invention. It is explanatory drawing which shows the state of the glass processing body vicinity at the time of applying a pressure to a blood collection bag using the pressurization apparatus of FIG.2 and FIG.9.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Serum collection system 2 Blood component separation apparatus 3 Pressurization apparatus 4 Component accommodating part support apparatus 5 Stand 5a Suspension hanger 30,40 Base 31,41 Fixed plate 32,42 Movable plate 32a, 42a Metal plate 32b, 42b Transparent plate 33 43 Lever 34, 44 Torsion spring 35, 45 Hook 36, 46 Lever hook 37 Elastic member 100 Blood collection bag 101, 201, 301 Main body part 102, 205, 305 Connection port 105 Glass processed body 200 Preparative bag 110, 210, 310 Hole 300 Adjustment bag 400, 401, 402, 403, 404, 405, 406 Connecting tube 450, 451, 452 Branch pipe 500, 501, 502, 503, 504 Clamp

Claims (4)

A blood storage part for storing a plurality of blood components therein, a connection pipe connected to the blood storage part for delivering a predetermined blood component to the outside of the blood storage part, a plurality of branch pipes branching from the connection pipe, and the plurality of A component separation device having a plurality of component storage units connected to the branch pipe;
A separation and pressurization device that applies pressure to the blood reservoir and moves blood components in the blood reservoir to the plurality of component storage units,
The plurality of component storage units are positioned below the blood storage unit, and a pressure applied to the blood storage unit and a pressure generated by a drop between the blood storage unit and the plurality of component storage units are within the blood storage unit. Blood component is collected from the blood reservoir by moving the blood component from the blood separated for each component to the plurality of component storage units via the connection pipe and the branch pipe. A blood component distribution device that subdivides into a plurality of component storage units,
The component separation device is connected to a branch pipe branched from the connection pipe, and has an adjustment reservoir capable of inflow and outflow of blood components,
The branch pipe is set to a length that allows the adjustment reservoir to be positioned above and below the plurality of component storage units,
The separation and pressurization device includes a pressurizing unit that applies pressure to the blood storage unit, a component storage unit support unit that is disposed below the blood storage unit and supports the plurality of component storage units, and the plurality of component storages An adjustment reservoir support means for supporting and detachably attaching the adjustment reservoir above the portion;
The component container support means includes a fixed plate for installing the plurality of component containers with the connecting portion between the branch pipe and each component container facing upward, a counter plate disposed opposite to the fixed plate, and the fixed plate A movable plate that opens and closes the plate surface, and a biasing means that biases the movable plate toward the fixed plate,
The blood component distribution device, wherein the movable plate simultaneously pressurizes the plurality of component storage portions when the plate surface of the fixed plate is closed. The blood reservoir has inserted a blood coagulation promoting individual that promotes blood coagulation inside,
The pressurizing means includes a fixed plate on which the blood reservoir is installed, a movable plate disposed opposite to the fixed plate and approaching and separating from the plate surface of the fixed plate, and the movable plate on the fixed plate side. And a biasing means for biasing
The blood component distribution device according to claim 1, wherein a concave portion is formed in a portion of the fixing plate facing the lower portion of the blood storage portion. The blood reservoir has inserted a blood coagulation promoting individual that promotes blood coagulation inside,
The pressurizing means includes a fixed plate on which the blood reservoir is installed, a movable plate disposed opposite to the fixed plate and approaching and separating from the plate surface of the fixed plate, and the movable plate on the fixed plate side. And a biasing means for biasing
The blood component distribution device according to claim 1, wherein a buffer member that abuts against the blood storage portion is provided on at least one of the fixed plate and the movable plate. A blood component distribution method using the blood component distribution device according to claim 1, 2, or 3, wherein a branch pipe of the adjustment reservoir is closed, and the blood reservoir is set in the pressurizing means. Pressurizing the blood reservoir and moving blood components in the blood reservoir to the plurality of component reservoirs;
Closing the connecting pipe connected to the blood reservoir, positioning the reservoir for adjustment below the plurality of component reservoirs, opening the branch pipe of the reservoir for adjustment, and accommodating the components Moving the air in the plurality of component storage parts and the air in the flow path from the plurality of component storage parts to the adjustment storage part to the adjustment storage part together with some of the blood components stored in the part; ,
Closing the branch pipe of the adjustment storage section, positioning the adjustment storage section above the plurality of component storage sections with the connecting portion between the adjustment storage section and the branch pipe facing downward, Opening the branch pipe of the adjustment reservoir and returning the blood components in the adjustment reservoir to the plurality of component reservoirs;
A blood component distribution method comprising: sealing the plurality of component accommodating portions and taking out the component accommodating portion supporting means.

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