JP4795765B2 - Sample processing equipment - Google Patents

Description

  The present invention relates to a sample processing apparatus, and more particularly to a sample processing apparatus for processing a sample such as a living tissue.

  Conventionally, as a sample processing apparatus for processing a sample such as a biological tissue, a filtering apparatus including a filtering tool, a homogenizer including a crushing tool (an apparatus for homogenizing a sample), and the like are known (for example, Patent Documents 1 and 2). reference).

  In Patent Document 1, by filtering a tissue solution (suspension) containing cell particles obtained by crushing a biological tissue, by collecting only cell particles larger than a predetermined size, A filtration device for producing a specimen slide glass to which cell particles larger than a predetermined size are attached is disclosed. The filter of Patent Document 1 is configured by a cylindrical body having one end closed and a filter provided at the other end. And in the said patent document 1, when producing a sample slide glass, after inserting a filter tool in the container in which the tissue solution was accommodated first, a tissue solution was made by making the inside of a filter tool into a negative pressure. Suction. Thereby, a filtrate is extracted inside the filter. At this time, cell particles smaller than a predetermined size pass through the filter and move into the filter, while cell particles larger than the predetermined size remain on the outer surface of the filter without passing through the filter. . Thereafter, the cell surface remaining on the outer surface of the filter is attached to the surface of the slide glass by bringing the outer surface of the filter of the filter tool into contact with the surface of the slide glass. In this way, a specimen slide glass is produced.

  Patent Document 2 discloses a homogenizer for producing a tissue solution containing a homogenized living tissue by homogenizing the living tissue with a crushing tool (pestle). Note that the homogenizer disclosed in Patent Document 2 only homogenizes living tissue.

JP-A-6-221976 JP 2000-333669 A

  However, in the filtration device of Patent Document 1, the filtrate is extracted into the inside of a filter made of a cylinder having one end closed and a filter provided at the other end. There is a problem that it is difficult to collect the filtrate.

  Further, the homogenizer of Patent Document 2 is not provided with a mechanism for filtering the tissue solution. Therefore, when filtering the tissue solution, there is a problem in that it is necessary to separately prepare a filtering device.

The present invention has been made to solve the above-described problems, and one object of the present invention is to prepare a tissue solution by crushing a living tissue , and then filtering the tissue solution to obtain a filtrate. It is providing the sample processing apparatus which performs extracting automatically .

Means for Solving the Problems and Effects of the Invention

To achieve the above object, the sample processing apparatus according to an aspect of the present invention, container for accommodating the biological tissue, crushing device for crushing the body tissue, and the tissue solution obtained by crushing the body tissue A sample processing apparatus for processing a sample using a filtering tool for filtering , wherein the storage container has a bottomed filtration chamber provided with an opening, a bottomed crushing chamber provided with an opening, and crushing before use The crushing tool storage chamber for storing the tools and the filter tool storage chamber for storing the filter tools before use are provided adjacent to each other in a row, and the crushing tool has an end for crushing the living tissue. The filtering tool has a cylindrical body having an opening at one end and a filter at the other end, and the sample processing apparatus includes a container mounting portion on which a storage container can be mounted, and a crushing tool. and out of the filtration device to hold at least one, and retained breaking device the times A holding member which includes a first moving mechanism for the movable holding member in the vertical direction, is movable in the vertical direction, a suction dispensing mechanism for dispenses aspirated matter at least tissue solution, the holding member A removing mechanism for removing at least one of the filtering tool and the crushing tool held by the holder from the holding member, and the container mounting portion is movable in a horizontal direction with respect to the holding member and the suction dispensing mechanism. The crushing tool accommodated in the storage container is held by the holding member by moving the holding member by the first moving mechanism, and the crushing tool held by the holding member by the first moving mechanism is moved. by, by is inserted from the end portion of the crushing device through the opening of the crushing chamber of the placed container to the container mounting part, rotating the held by the holding member crushing tool, raw Filtration chamber in which tissue solution is prepared by crushing tissue to suck tissue solution from the crushing chamber of the storage container by the suction dispensing mechanism, and the aspirated tissue solution is provided separately from the crushing chamber of the storage container The crushing tool held by the holding member is removed from the holding member by the removing mechanism section, and the holding tool is moved by the first moving mechanism section, thereby holding the filtering tool stored in the receiving container. By holding the member and moving the filter held by the holding member by the first moving mechanism , the filter is inserted from the other end through the opening of the filtration chamber of the storage container, and the tissue solution is filtered. Extract the filtrate.

In the sample processing apparatus according to this one aspect, by providing the above-described configuration, a biological tissue is crushed to prepare a tissue solution, and the tissue solution is filtered to extract a filtrate automatically. be able to.

  In the sample processing apparatus according to the first aspect, preferably, the filtrate is extracted into a cylindrical body constituting the filter. If comprised in this way, the filtrate extracted inside the filter tool can be easily attracted | sucked through opening of a filter tool.

In sample processing apparatus according to the aforementioned aspect preferably, the container platform is further a collection container for containing the extracted filtrate is configured to be placed, dispensing mechanism unit suction amount is by filtration instrument It has the function of sucking the extracted filtrate and dispensing the sucked filtrate into a collection container. If comprised in this way, a filtrate can be extract | collected easily by a suction dispensing mechanism part.

In sample processing apparatus according to the aforementioned aspect preferably, the holding member, than the placed container to the container platform is located above, while holding the crushing tool by hold member, the first movement By moving the holding member downward by the mechanism and inserting the crushing tool into the crushing chamber of the storage container, the living tissue is crushed and the first moving mechanism is held by the holding member. Thus, the tissue solution is filtered by moving the holding member downward and inserting the filter into the filtration chamber of the storage container. If comprised in this way, when the holding member is arrange | positioned upwards rather than a storage container, a biological tissue will be easily crushed and moved by moving a holding member below by a 1st moving mechanism part. The solution can be adjusted and the tissue solution can be filtered.

In the sample processing apparatus according to the above aspect , preferably, the holding member includes a press-fitting portion into which the filtering tool and the crushing tool are press-fitted. If comprised in this way, a filter and a crushing tool can be easily hold | maintained with a holding member by press-fitting a filter and a crushing tool in the press-fit part of a holding member.

In this case, preferably, by moving the Riho support member by the first moving mechanism downward, the container platform is accommodated in the placed container to the filtration equipment and crushing device said holding member It is press-fitted into the press-fitting part. If comprised in this way, the pre-use filter tool and crushing tool accommodated in the storage container can be press-fitted and held in the press-fitting part of the holding member by moving the holding member downward. it can.

In the configuration in which the holding member includes a press-fitting part into which the filtering tool and the crushing tool are press-fitted, preferably, the detaching mechanism unit removes at least one of the filtering tool and the crushing tool press-fitted into the press-fitting part of the holding member from the holding member. to the outside to take. If comprised in this way, when changing from the preparation process of a tissue solution to the filtration process of a tissue solution, a crushing tool can be removed from a holding member by a removal mechanism part, and a filtering tool can be mounted | worn. Further, when shifting from the tissue solution filtration step to the tissue solution preparation step, the removal tool can remove the filter tool from the holding member and attach the crushing tool.

  In the configuration further including the removal mechanism, the removal mechanism preferably holds the removal member by removing the filter member and the crushing tool from the holding member, and moving the removal member in the horizontal direction. A second moving mechanism for disposing the member at a position near the press-fitting portion, and moving the removing member in the horizontal direction by the second moving mechanism and disposing the first moving mechanism at the removing position. By moving the holding member upward by this, at least one of the filtering tool and the crushing tool is removed from the holding member. With this configuration, even if the holding member for holding the filtering tool and the crushing tool is configured to be movable only in the vertical direction, the holding member is moved in the vertical direction and the removal member is moved in the horizontal direction. Thus, at least one of the filtering tool and the crushing tool can be easily detached from the holding member.

In sample processing apparatus according to the aforementioned aspect preferably, the container platform, the horizontal position of the filtration chamber of the container placed on the holding member and the container mounting part which is held filtration device substantially And the second position where the horizontal position of the holding member holding the crushing tool and the crushing chamber of the storage container placed on the container placing portion substantially coincide with each other. further comprising a third moving mechanism, for causing the at least moved to. With this configuration, even if the holding member is configured to be movable only in the vertical direction, if the storage container mounting member on which the storage container is mounted is moved in the horizontal direction by the third movement mechanism unit, the tissue solution When performing filtration, the horizontal position of the holding member holding the filter and the filtration chamber of the container can be matched, and holding the crushing tool when preparing the tissue solution The horizontal position of the member and the crushing chamber of the storage container can be matched. Thus, even when the holding member is configured to be movable only in the vertical direction, when filtering the tissue solution, the filtering tool can be inserted into the filtration chamber of the storage container and the tissue solution is prepared. In some cases, the crushing tool can be inserted into the crushing chamber of the receiving container.

  Hereinafter, embodiments of the present invention will be described with reference to the drawings.

  FIG. 1 is a perspective view showing the overall configuration of a sample processing apparatus according to an embodiment of the present invention. FIG. 2 is a perspective view of a sample preparation kit attached to the sample processing apparatus according to the embodiment shown in FIG. 1, and FIG. 3 is a sample attached to the sample processing apparatus according to the embodiment shown in FIG. It is an exploded view of a preparation kit. 4 to 14 are views for explaining the detailed structure of the sample preparation kit attached to the sample processing apparatus according to the embodiment shown in FIG.

  As shown in FIG. 1, the sample processing apparatus 100 according to the present embodiment is an apparatus for collecting a filtrate by crushing a biological tissue and then filtering a tissue solution obtained from the crushed biological tissue. It is. In addition, a lymph node is used as a biological tissue. In addition, the lymph nodes that have been cut in advance are accommodated in the sample preparation container 10 of the sample preparation kit 1 and then placed in the sample processing apparatus 100 shown in FIG. First, with reference to FIGS. 2 to 14, the configuration of the sample preparation kit 1 attached to the sample processing apparatus 100 according to the present embodiment will be described in detail.

  As shown in FIGS. 2 and 3, the sample preparation kit 1 attached to the sample processing apparatus 100 according to the present embodiment includes a sample preparation container 10, a lid 20 fitted into the sample preparation container 10, and a cut lymph. A crushing tool 30 for crushing a node, a filtering tool 40 for filtering a tissue solution obtained from a lymph node, and two dispensing tips 50 for sucking and discharging the filtrate filtered by the filtering tool 40 And 60. In the sample preparation container 10 of the sample preparation kit 1, a crushing solution 70 and a diluting solution 80 are stored in advance. The crushing solution 70 and the diluting solution 80 contain a nonionic surfactant that breaks down membrane proteins contained in lymph nodes. Further, as shown in FIGS. 3 and 4, the upper surface portion 17 (see FIGS. 5 and 6) of the sample preparation container 10 is configured so that dust from the outside does not enter the crushing solution 70 and the diluting solution 80. A film 90 made of aluminum is adhered. The sample preparation kit 1 is a disposable kit that is discarded after each use.

  As shown in FIGS. 3 to 6, the sample preparation container 10 includes a filtering tool storage chamber 11 for storing the filtering tool 40, a crushing tool storage chamber 12 for storing the crushing tool 30, and a crushing solution 70. Including a crushing processing storage chamber 13 in which the diluting liquid 80 is stored, and a dispensing tip storage chamber 15 for storing the two dispensing tips 50 and 60. Yes. And each accommodating chamber 11-15 is integrally formed via the reinforcement rib 16 and the upper surface part 17, as shown in FIG. And the filtration tool storage chamber 11, the crushing tool storage chamber 12, the crushing processing storage chamber 13, the filtration processing storage chamber 14, and the dispensing tip storage chamber 15, respectively, have openings 11a, 12a, 13a, 14a and It has 15a and has bottom surface portions 11b, 12b, 13b, 14b and 15b at the other end. Moreover, as shown in FIG. 3 and FIG. 6, the side part 18 formed so that the circumference | surroundings of each storage chamber 11-15 may be surrounded, the crushing tool storage chamber 12, the crushing processing storage chamber 13, and the filtration processing storage chamber 14 and the dispensing tip storage chamber 15 are connected to each other by the reinforcing rib 16 described above. Further, the reinforcing ribs 16 are provided between the filtration tool storage chamber 11 and the crushing tool storage chamber 12, between the crushing tool storage chamber 12 and the crushing processing storage chamber 13, and between the crushing processing storage chamber 13 and the filtration processing storage chamber. 14 and between the filtration processing storage chamber 14 and the dispensing tip storage chamber 15. Further, the upper surface portion 17 is provided with an edge portion 19 extending vertically upward so as to surround the upper surface portion 17, and the edge portion 19 on the filter tool storage chamber 11 side and the dispensing tip storage chamber 15 side is provided. Protrusions 19a and 19b are provided on the outer side surfaces, respectively.

  As shown in FIG. 3, the filter tool storage chamber 11 is provided to store the filter tool 40 of the sample preparation kit 1, and the crushing tool storage chamber 12 is provided to store the crush tool 30. . Further, as shown in FIG. 5 and FIG. 6, the filtering tool storage chamber 11 and the crushing tool storage chamber 12 are each a support surface portion 11 c having an oval shape as viewed in a plane depressed by a predetermined depth from the upper surface portion 17. And 12c are provided. Further, the filter tool housing chamber 11 is formed with notches 11d and 11e. The crusher accommodation chamber 12 is also formed with notches 12d and 12e. And the notch part 11e by the side of the crushing tool accommodation chamber 12 of the filter tool accommodation chamber 11 and the notch part 12e by the side of the filtration tool accommodation chamber 11 of the crushing tool accommodation chamber 12 are formed so that it may connect.

  The crushing processing chamber 13 is provided for crushing the lymph nodes with the crushing tool 30 received from the opening 13a, and the lymph nodes cut in advance together with the crushing solution 70 are used for this crushing treatment. It is stored in the storage chamber 13. In the sample preparation kit 1 of the present embodiment, the lymph node is crushed in the crushing solution 70 in the crushing processing chamber 13, and a tissue solution is obtained. Moreover, as shown in FIG.7 and FIG.8, the semicircular recessed part 31a of the flat inner crushing member 31 of the crushing tool 30 mentioned later (FIG. 9), a semicircular convex portion 13c is formed. The convex portion 13c has a cross-shaped rib shape when seen in a plan view. The convex portion 13c and the concave portion 31a of the inner crushing member 31 are configured to sandwich and crush a lymph node having a predetermined size. Further, the radius of curvature of the surface of the convex portion 13 c is formed to have substantially the same radius of curvature as the surface of the concave portion 31 a of the inner crushing member 31 of the crushing tool 30. Furthermore, as shown in FIG. 8, when the concave portion 31 a provided at the flat tip portion of the inner crushing member 31 of the crushing tool 30 rotates with respect to the cross-shaped rib-shaped convex portion 13 c, the inner crushing member 31. A gap 13d is formed between the concave portion 31a provided at the flat plate-like tip and a portion other than the cross-shaped rib-shaped convex portion 13c.

  The filtration chamber 14 is provided to receive the tissue solution obtained by crushing the lymph nodes and to filter the received tissue solution. In the sample preparation kit 1 of the present embodiment, the tissue solution sucked in the crushing processing chamber 13 is dispensed in the filtration processing chamber 14, so that the dispensed tissue solution is preliminarily stored. Dilution is performed with the diluent 80 contained therein. Then, the filtrate is extracted by filtering the diluted tissue solution. Further, as shown in FIGS. 3 and 6, the filtration storage chamber 14 is formed so that the opening cross-sectional area gradually decreases from the opening 14 a side toward the bottom surface portion 14 b side.

  Dispensing tip accommodating portions 15c and 15d are formed in the dispensing tip accommodating chamber 15 so that the dispensing tips 50 and 60 can be accommodated, respectively.

  As shown in FIGS. 2 and 3, the lid 20 is configured to be fitted into the sample preparation container 10. Specifically, the lid 20 is provided with a locking hole 20a corresponding to a projection 19a provided on the filter device storage chamber 11 side of the edge 19 of the sample preparation container 10, and the dispensing tip storage chamber 15 is provided. A locking hole 20b corresponding to the protruding portion 19b provided on the side is provided. As a result, the projections 19a and 19b of the sample preparation container 10 are engaged with the locking holes 20a and 20b of the cover part 20, respectively, so that the cover part 20 can be fitted into the sample preparation container 10. . The lid 20 includes a recess 20 c formed so as to correspond to the opening 13 a of the crushing processing chamber 13. The recess 20c corresponding to the opening 13a of the crushing processing chamber 13 has a function of closing the opening 13a of the crushing processing chamber 13 when the lid 20 is fitted into the sample preparation container 10. Yes. This makes it possible to suppress leakage of the remaining tissue solution from the opening 13a of the crushing processing chamber 13 in the state where the lid 20 is fitted into the sample preparation container 10 after the tissue solution is collected. .

  As shown in FIGS. 9 and 10, the crushing tool 30 includes an inner crushing member 31 and an outer crushing member 32 made of a cylinder that can accommodate the inner crushing member 31 therein. And the crushing tool 30 is provided in order to crush the lymph node accommodated with the solution 70 for crushing. Further, the inner crushing member 31 includes a concave portion 31a formed in a flat tip portion so as to correspond to the convex portion 13c (see FIGS. 7 and 8) of the crushing processing chamber 13 of the sample preparation container 10. It is out. As described above, the surface of the concave portion 31a provided at the flat tip portion is formed to have a curvature radius substantially the same as the curvature radius of the surface of the convex portion 13c of the crushing processing chamber 13. . Further, on the side opposite to the distal end side where the concave portion 31a of the inner crushing member 31 is formed, a support portion 31b that is gripped by a grip portion of the sample processing apparatus 100 described later is provided. Further, as shown in FIG. 9, on the lower side of the outer crushing member 32, a plurality of sword portions 32a, a rib portion 32b formed so as to protrude outward from the outer peripheral surface side, and a plurality of sword portions 32a A plurality of slits 32c provided therebetween are provided. Further, on the upper side of the outer crushing member 32, as shown in FIG. 9 and FIG. 11, an oval shape corresponding to the oval support surface portion 12c (see FIGS. 5 and 6) of the crusher accommodation chamber 12 is provided. A portion 32d, an opening 32e for accommodating the inner crushing member 31 therein, two notches 32f, and a plurality of (four in this embodiment) locking claws 32g that can be deformed are formed. ing. The two notches 32f are formed so as to correspond to the notches 12d and 12e (see FIGS. 5 and 6) formed in the crushing tool storage chamber 12.

  The filter 40 is provided for filtering the tissue solution obtained from the crushed lymph node, and for extracting the filtrate filtered through the two filters 41 and 42 (see FIG. 13). Used. As shown in FIGS. 12 to 14, the filter 40 has a cylindrical shape, and on the lower side, a filter installation part 40 a for arranging two filters 41 and 42, and a seal material installation part 40b. In the present embodiment, the sealing material is not disposed in the sealing material installation portion 40b, but a sealing material can also be disposed. Moreover, as shown in FIG. 14, the filter installation part 40a is formed with a cross-shaped filter support part 40c in plan view. Then, the tissue solution is extracted into the filter 40 through the gap 43 other than the portion where the filter support portion 40 c of the filter installation portion 40 a is formed. Moreover, the upper side of the filter 40 has the same structure as the upper side of the outer crushing member 32 described above, as shown in FIGS. 11c (see FIG. 5 and FIG. 6), a flange portion 40d having an oval shape, an opening 40e, two notches 40f, and a plurality of flexibly deformable (four in this embodiment). ) Locking claws 40g. The notch 40f is formed so as to correspond to the notches 11d and 11e (see FIGS. 5 and 6) formed in the filter accommodation chamber 11.

  The dispensing tip 50 accommodated in the dispensing tip accommodating portion 15c is provided for aspirating and dispensing a tissue solution obtained from a crushed lymph node. Moreover, the dispensing tip 60 accommodated in the dispensing tip accommodating part 15d is provided in order to suck and dispense the filtrate extracted by filtering the tissue solution. As shown in FIG. 3, the dispensing tip 50 includes an opening 50a and a tip 50b opposite to the opening 50a. Similarly, the dispensing tip 60 includes an opening 60a and a tip 60b opposite to the opening 60a.

  15 and 16 are a perspective view and a plan view, respectively, of the main body of the sample processing apparatus according to the embodiment shown in FIG. 17 to 24 are diagrams showing the detailed structure of each part of the sample processing apparatus according to the embodiment shown in FIG. Next, referring to FIGS. 1, 3, 6, 9, 10, 12, and 14 to 24, the sample processing apparatus 100 according to this embodiment to which the above-described sample preparation kit 1 is attached is described. The configuration will be described in detail.

  In the sample processing apparatus 100 according to the present embodiment, the lid 20 (see FIG. 3) and the film 90 (see FIG. 3) are removed, and the sample preparation kit 1 containing the previously cut lymph nodes is installed, The filtrate is collected by performing a predetermined process on the lymph nodes to be accommodated. Specifically, as shown in FIG. 1, the sample processing apparatus 100 according to the present embodiment includes a display unit 110 for displaying predetermined information and three main body units 120. As shown in FIGS. 15 and 16, the main body unit 120 includes a transport mechanism unit 130, a sample processing mechanism unit 140, a suction / dispensing mechanism unit 150, a removal mechanism unit 160, and a control unit 170. Has been. In addition, the sample processing apparatus 100 is provided with an eject button 180. When the eject button 180 is pressed, the installation unit 131 of the transport mechanism unit 130 is moved in the horizontal direction (X1 direction) and carried out from the door unit 190 to the outside. Thereby, the sample preparation kit 1 can be installed in the sample processing apparatus 100.

  The transport mechanism unit 130 includes an installation unit 131 and a movement mechanism unit 132. The transport mechanism unit 130 has a function of installing the above-described sample preparation kit 1 and also has a function of moving the sample preparation kit 1 in the horizontal direction (X1 and X2 directions).

  The installation part 131 is provided for installing the sample preparation kit 1 and for installing the collection container 300 for storing the filtrate filtered by the filter 40. As shown in FIGS. 17 and 18, the installation unit 131 includes a kit installation unit 131 a for installing the sample preparation kit 1, a collection container installation unit 131 b for installing the collection container 300, and the sample preparation kit 1. The temperature control part 131c (refer FIG. 17) for controlling the tissue solution accommodated and the filtrate accommodated in the collection | recovery container 300 to predetermined | prescribed temperature is comprised.

  Moreover, the kit installation part 131a accommodates the filtering tool storage chamber 11, the crushing tool storage chamber 12, the crushing processing storage chamber 13, the filtration processing storage chamber 14, and the dispensing tip storage chamber 15 of the sample preparation container 10, respectively. Insertion portions 131d, 131e, 131f, 131g and 131h are provided. The kit installation portion 131a is provided with a rib insertion groove 131i formed so as to correspond to the reinforcing rib 16 of the sample preparation container 10.

  Moreover, the collection container installation part 131b suppresses the container insertion hole 131j (see FIG. 18) for installing the collection container 300 and the lid 301 attached to the collection container 300 from blocking the opening 300a of the collection container 300. And a lid holding part 131k provided for this purpose. In addition, the temperature control unit 131c is provided with two fans 131l and a Peltier element (not shown) is built in. A control signal transmitted from the control unit 170 (see FIGS. 15 and 16), which will be described later, is transmitted. Based on this, the two fans 131l and the Peltier element are controlled.

  As shown in FIGS. 15 and 16, the moving mechanism unit 132 is provided to move the installation unit 131 in which the sample preparation kit 1 is arranged in the horizontal direction (X1 and X2 directions). The movement mechanism unit 132 includes a motor 132a, a pulley 132b connected to the motor 132a, a pulley 132c provided at a predetermined interval from the pulley 132b, and a drive transmission belt 132d attached to the pulley 132b and the pulley 132c. The linear motion guide 132e is disposed so as to extend in the horizontal direction (X1 and X2 directions), and the movable member 132f is movably attached along the linear motion guide 132e and coupled to the drive transmission belt 132d. ing. Thus, when the motor 132a is driven, the drive transmission belt 132d is driven via the pulley 132b, so that the moving member 132f connected to the drive transmission belt 132d is moved in the horizontal direction (X1 and X2 directions). The And it becomes possible to move the installation part 131 attached to the moving member 132f along the linear motion guide 132e with the moving member 132f.

  The sample processing mechanism unit 140 uses the crushing tool 30 (see FIG. 9) and the filtering tool 40 (see FIG. 12) housed in the sample preparation kit 1 to crush the lymph nodes housed in the sample preparation kit 1. It has a function of filtering the tissue solution obtained by crushing. The sample processing mechanism unit 140 includes a holding member 141 and a moving mechanism unit 142.

  The holding member 141 has a function of holding the crushing tool 30 and the filtering tool 40 of the sample preparation kit 1. As shown in FIG. 19, the holding member 141 is provided with a mounting part 141a for mounting the crushing tool 30 and the filtering tool 40, a motor 141b provided on the upper side of the mounting part 141a, a motor 141b, and a mounting part 141a. An installation portion 141c for mounting, a sheet metal 141d attached to the installation portion 141c, a linear motion guide 141e attached to the metal plate 141d, a moving member 141f attached movably along the linear motion guide 141e, and a moving member 141f A hook member 141g to be attached and a spring 141h are included.

  As shown in FIG. 20, the mounting portion 141 a includes a press-fit portion 141 i that is press-fitted from the opening 32 e side of the outer crushing member 32 of the crushing tool 30 and the opening 40 e (see FIG. 14) side of the filtering tool 40, and crushing It includes a notch 32 f of the outer crushing member 32 of the tool 30 and a protrusion 141 j that engages with the notch 40 f (see FIG. 14) of the filter 40. In addition, the press-fit portion 141i is provided with a groove portion 141k that engages with the locking claw 32g of the outer crushing member 32 of the crushing tool 30 and the locking claw 40g (see FIG. 12) of the filtering tool 40. As a result, the press-fitting portion 141i of the mounting portion 141a is press-fitted into the opening 32e of the outer crushing member 32 and the opening 40e of the filter 40, and the locking claw 32g and the locking claw 40g are engaged with the groove 141k of the press-fitting portion 141i. Accordingly, the crushing tool 30 and the filtering tool 40 can be securely held by the mounting portion 141a. At this time, the protrusion 141j of the mounting portion 141a engages with the cutout portion 32f of the outer crushing member 32 and the cutout portion 40f of the filter 40, so that the outer crushing member 32 and the filter 40 rotate with respect to the mounting portion 141a. It is possible to suppress this. Further, inside the press-fit portion 141i, a grip portion (not shown) that rotates in accordance with the driving of the motor 141b shown in FIG. 19 is provided, and the support portion 31b (see FIG. 9 and FIG. (See FIG. 10). Therefore, the motor 141b functions as a drive source for rotating the inner crushing member 31 of the crushing tool 30.

  As shown in FIG. 19, a hook part 141l for attaching one end of a spring 141h is integrally formed on a sheet metal 141d attached to the installation part 141c. The other end of the spring 141h is attached to the hook member 141g. Accordingly, the moving member 141f can be moved in the vertical direction (Z1 and Z2 directions) so as to follow the movement of the mounting portion 141a in the vertical direction (Z1 and Z2 directions). The moving member 141f is attached with a resin contact portion 141m that contacts a release claw 161a of a removal member 161 of a removal mechanism portion 160 described later. Further, the contact portion 141m contacts the flange portion 32d (see FIG. 9) of the outer crushing member 32 and the flange portion 40d of the filter device 40 when the crusher 30 and the filter device 40 are mounted on the mounting portion 141a. It is attached to the moving member 141f.

  As shown in FIGS. 15 and 16, the moving mechanism 142 is provided to move the holding member 141 in the vertical direction (Z1 and Z2 directions). The moving mechanism 142 includes a motor 142a, a pulley 142b connected to the motor 142a, a pulley 142c provided at a predetermined interval from the pulley 142b, and a drive transmission belt 142d attached to the pulley 142b and the pulley 142c. A ball screw 142e that rotates with the rotation of the pulley 142c and extends in the vertical direction (Z1 and Z2 directions), and a linear motion guide 142f that extends in the vertical direction (Z1 and Z2 directions). The moving member 142g is attached to the ball screw 142e so as to move along with the linear motion guide 142f. Accordingly, when the motor 142a is driven, the drive transmission belt 142d is driven via the pulley 142b, and thus the pulley 142c to which the drive transmission belt 142d is attached rotates. For this reason, since the ball screw 142e is rotated with the rotation of the pulley 142c, the moving member 142g that moves with the rotation of the ball screw 142e moves along the vertical direction (Z1 and Z2 directions) in which the linear motion guide 142f extends. Is done.

  The suction / dispensing mechanism unit 150 attaches the dispensing tips 50 and 60 (see FIG. 3) of the sample preparation kit 1 to suck and dispense the tissue solution and suck the filtrate extracted from the tissue solution. And has the function of dispensing. The suction dispensing mechanism unit 150 includes a syringe unit 151 that sucks and discharges tissue solution and filtrate, and a moving mechanism unit 152 that moves the suction dispensing mechanism unit 150 in the vertical direction (Z1 and Z2 directions). It is configured.

  As shown in FIGS. 21 and 22, the syringe part 151 includes a nozzle part 151a to which the dispensing tips 50 and 60 (see FIG. 3) are attached, a resin cap 151b attached to the nozzle part 151a, and suction and discharge. For detecting whether or not the pump portion 151c for performing the operation, the motor 151d as a drive source of the pump portion 151c, the tip portion 50b of the dispensing tip 50 and the tip portion 60b of the dispensing tip 60 are in contact with the liquid surface Including a liquid level detection sensor 151e, a pressure detection sensor 151f for detecting pressure during suction and discharge by the pump unit 151c, and a control board 151g for controlling the liquid level detection sensor 151e and the pressure detection sensor 151f. It is out. Further, the dispensing tips 50 and 60 are mounted by press-fitting the tip portion 151h of the nozzle portion 151a into the opening 50a of the dispensing tip 50 and the opening 60a (see FIG. 3) of the dispensing tip 60. The resin cap 151b is attached to the nozzle portion 151a so as to be movable in the vertical direction (Z1 and Z2 directions). The resin cap 151b is formed with an edge portion 151i that comes into contact when the dispensing tips 50 and 60 are mounted.

  As shown in FIGS. 15 and 16, the moving mechanism unit 152 includes a motor 152a, a pulley 152b connected to the motor 152a, a pulley 152c provided at a predetermined interval from the pulley 152b, a pulley 152b, and a pulley. A drive transmission belt 152d attached to 152c, a ball screw 152e that rotates with the rotation of the pulley 152c and that extends in the Z direction, and a straight screw that extends in the vertical direction (Z1 and Z2 directions). The moving guide 152f includes a moving member 152g that is moved along with the rotation of the ball screw 152e and is movably attached along the linear moving guide 152f. Accordingly, when the motor 152a is driven, the drive transmission belt 152d is driven via the pulley 152b, and thus the pulley 152c to which the drive transmission belt 152d is attached rotates. For this reason, since the ball screw 152e is rotated with the rotation of the pulley 152c, the moving member 152g that moves with the rotation of the ball screw 152e moves along the vertical direction (Z1 and Z2 directions) in which the linear motion guide 152f extends. Is done.

  The removal mechanism unit 160 includes the crushing tool 30 (see FIG. 9) and the filtering tool 40 (see FIG. 12) held by the holding member 141, and the dispensing tips 50 and 60 (see FIG. 12) attached to the suction dispensing mechanism unit 150. 3). Furthermore, the removal mechanism 160 suppresses the tissue solution dripped from the used crushing tool 30 from adhering to each part of the sample preparation container 10 and the sample processing apparatus 100, and dripped from the used dispensing tips 50 and 60. It also has a function of suppressing the tissue solution and filtrate to be attached to each part of the sample preparation container 10 and the sample processing apparatus 100. As shown in FIGS. 15, 16, and 23, the detaching mechanism unit 160 includes a detaching member 161 for detaching the crushing tool 30 and the filtering tool 40 from the holding member 141, and the detaching member 161 in the horizontal direction (X1 and X2 directions). And a moving mechanism portion 162 for moving to ().

  As shown in FIGS. 23 and 24, the detaching member 161 has a release claw 161a that contacts the upper surface of the contact portion 141m of the holding member 141 and an edge portion 151i of the cap 151b of the suction dispensing mechanism portion 150. The tip release hole 161b and a resin tray 161c that receives the tissue solution and filtrate are included.

  As shown in FIGS. 15, 16 and 23, the moving mechanism unit 162 includes a motor 162a, a pulley 162b connected to the motor 162a, a pulley 162c provided at a predetermined interval from the pulley 162b, a pulley 162b and a drive transmission belt 162d attached to the pulley 162c, a linear motion guide 162e arranged to extend in the horizontal direction (X1 and X2 directions), and a moving member 162f attached to be movable along the linear motion guide 162e. It is comprised by. As a result, when the motor 162a is driven, the drive transmission belt 162d is driven via the pulley 162b, so that the moving member 162f coupled to the drive transmission belt 162d is moved in the horizontal direction (X1 and X2 directions). The For this reason, the detaching member 161 attached to the moving member 162f can move in the horizontal direction (X1 and X2 directions).

  As shown in FIGS. 15 and 16, the control unit 170 is provided to control each unit of the sample processing apparatus 100. Specifically, the control unit 170 is electrically connected to the motors 132a, 142a, 152a, and 162a of the moving mechanism units 132, 142, 152, and 162, and drives the motors 132a, 142a, 152a, and 162a. Has a function of transmitting a control signal for controlling. Thereby, the movement of the installation part 131, the holding member 141, the syringe part 151, and the detaching member 161 is controlled. The control unit 170 is electrically connected to the fan 131l and the Peltier element (not shown) of the temperature control unit 131c of the installation unit 131, and a control signal for controlling the rotation of the fan 131l and the Peltier element. It has the function to transmit. Thereby, the tissue solution accommodated in the sample preparation kit 1 and the filtrate accommodated in the collection container 300 can be controlled to a predetermined temperature.

  25 to 50 are views for explaining the filtrate collecting operation of the sample processing apparatus according to the embodiment of the present invention. Next, with reference to FIGS. 3, 12, 14 to 16, and 19 to 50, the filtrate collecting operation of the sample processing apparatus 100 according to the present embodiment will be described.

  First, the lid 20 (see FIG. 3) and the film 90 (see FIG. 3) are removed from the sample preparation container 10 (see FIG. 3), and the previously cut lymph node 200 is housed in the crushing treatment storage chamber 13. A sample preparation kit 1 is prepared. And the prepared sample preparation kit 1 is installed in the installation part 131 discharged | emitted by pushing the eject button 180 of the sample processing apparatus 100 outside. Then, as shown in FIG. 25, by driving the motor 132a (see FIGS. 15 and 16) of the moving mechanism section 132, the position in the horizontal direction (X direction) of the mounting section 141a of the holding member 141 and the crushing tool The installation portion 131 is moved in the X1 direction so that the horizontal position of the crushing tool storage chamber 12 in which 30 is stored matches. In this state, the holding member 141 is moved in the Z1 direction (downward) by driving the motor 142a (see FIGS. 15 and 16) of the moving mechanism unit 142. At this time, as shown in FIG. 20, the cutout portion 32f of the outer crushing member 32 engages with the projection 141j of the mounting portion 141a, and the engaging claw 32g engages with the groove portion 141k of the mounting portion 141a. The crushing tool 30 is press-fitted to the part 141a. Thereafter, by moving the holding member 141 in the Z2 direction (upward), the state shown in FIG. 26 is obtained.

  And as shown in FIG. 27, the horizontal direction (X direction) position of the mounting part 141a to which the crushing tool 30 is mounted, and the crushing processing storage chamber 13 in which the lymph node 200 and the crushing solution 70 are stored. The installation unit 131 is moved in the X1 direction so that the position in the horizontal direction matches. Then, the lymph node 200 is crushed in the crushing storage chamber 13 by moving the holding member 141 in the Z1 direction (downward). Specifically, first, as shown in FIG. 28, the lymph node 200 is crushed until it reaches a predetermined size by repeatedly moving the crushing tool 30 in the Z1 direction (downward) and the Z2 direction (upward). Thereafter, as shown in FIG. 29, the inner crushing member 31 is driven in the crushing solution 70 in a state where the lymph node 200 having a predetermined size is floated by driving the motor 141b (see FIG. 19) of the holding member 141. Rotate. Thereby, as shown in FIG. 30, a tissue solution in which the lymph node 200 is further refined and homogenized is obtained in the crushing solution 70.

  Next, as shown in FIG. 31, the crushing tool 30 is pulled up from the tissue solution by moving the mounting portion 141a to which the crushing tool 30 is mounted in the Z2 direction (upward). Then, by driving the motor 162a (see FIGS. 15, 16, and 23) of the moving mechanism unit 162, the detaching member 161 is moved in the X2 direction to a position below the raised crushing tool 30. At this time, even when the tissue solution is dropped from the crushing tool 30 pulled up from the tissue solution, the tray 161c (see FIGS. 23 and 24) of the removing member 161 can receive the dropped tissue solution. .

  And as shown in FIG. 32, the installation part 131 so that the horizontal direction (X direction) position of the mounting part 141a to which the crushing tool 30 is mounted and the horizontal position of the crushing tool storage chamber 12 coincide. Is moved in the X2 direction, and the detaching member 161 is moved in the X1 direction. Thereafter, as shown in FIG. 33, the crushing tool 30 is inserted into the crushing tool storage chamber 12 by moving the holding member 141 in the Z1 direction (downward). Further, by moving the detaching member 141 in the X2 direction, the detaching member 141 is arranged at a detaching position in the vicinity of the holding member 141. Specifically, as shown in FIG. 33, the holding member 141 is moved in the Z1 direction (downward), and the release claw 161a of the removal member 161 is positioned above the contact portion 141m of the holding member 141. Then, the removal member 161 is moved in the X2 direction. In this state, by moving the holding member 141 in the Z2 direction (upward), the flange portion 32d of the crushing tool 30 presses the lower portion of the contact portion 141m. A force in the Z1 direction (downward) (direction in which the crushing tool 30 is removed from the mounting portion 141a) is applied from the contact portion 141m, and the outer crushing member 32 (crushing tool 30) is detached from the mounting portion 141a.

  Next, as shown in FIG. 34, the position of the nozzle portion 151a of the syringe portion 151 in the horizontal direction (X direction) and the dispensing tip storage portion 15c of the dispensing tip storage chamber 15 in which the dispensing tips 50 are stored. The installation unit 131 is moved in the X1 direction so that the position in the horizontal direction matches. In this state, the syringe unit 151 is moved in the Z1 direction (downward) by driving the motor 152a (see FIGS. 15 and 16) of the moving mechanism unit 152. At this time, as shown in FIG. 22, the opening 50a of the dispensing tip 50 is press-fitted into the tip portion 151h of the nozzle portion 151a, and the dispensing tip 50 is attached to the nozzle portion 151a. Then, by moving the syringe part 151 in the Z2 direction (upward), the state shown in FIG. 35 is obtained.

  As shown in FIG. 36, the position in the horizontal direction (X direction) of the nozzle portion 151a to which the dispensing tip 50 is attached and the position in the horizontal direction of the crushing treatment storage chamber 13 in which the tissue solution is stored. The installation unit 131 is moved in the X1 direction so as to match. And as shown in FIG. 37, while moving the syringe part 151 to Z1 direction (downward), the tissue solution in the storage chamber 13 for crushing process is attracted | sucked. Specifically, it is detected by the liquid level detection sensor 151e (see FIG. 21) that the tip 50b of the dispensing tip 50 is in contact with the liquid level of the tissue solution, and the motor 151d (see FIG. 21) is driven. The pump unit 151c (see FIG. 21) uses the force to suck the tissue solution. Then, the dispensing tip 50 is pulled up from the tissue solution by moving the nozzle portion 151a to which the dispensing tip 50 is attached in the Z2 direction (upward). Then, the removal member 161 is moved in the X2 direction to a position below the pulled dispensing tip 50. Thereafter, the installation portion 131 is moved in the X1 direction so that the horizontal position (X direction) of the nozzle portion 151a to which the dispensing tip 50 is attached coincides with the horizontal position of the filtration treatment storage chamber 14. Then, the removal member 161 is moved in the X1 direction.

  Thereafter, as shown in FIG. 38, the syringe unit 151 is moved in the Z1 direction (downward), and the tissue solution sucked in the crushing processing chamber 13 is discharged and stirred in the filtering processing chamber 14. Specifically, it is detected by the liquid level detection sensor 151e (see FIG. 21) that the tip 50b of the dispensing tip 50 is in contact with the liquid level of the diluent, and the motor 151d (see FIG. 21) is driven. The pump unit 151c (see FIG. 21) discharges the tissue solution using the force. At this time, the tissue solution is agitated by repeatedly performing suction and discharge in the diluted solution from which the tissue solution is discharged using the pump unit 151c.

  Then, the dispensing tip 50 is pulled up from the diluted tissue solution by moving the nozzle portion 151a to which the dispensing tip 50 is attached in the Z2 direction (upward). Then, the removal member 161 is moved in the X2 direction to a position below the pulled dispensing tip 50. Then, the horizontal position (X direction) of the nozzle portion 151a to which the dispensing tip 50 is attached and the horizontal position of the dispensing tip storage portion 15c of the dispensing tip storage chamber 15 are set to coincide with each other. The part 131 is moved in the X1 direction, and the detaching member 161 is moved in the X1 direction.

  Then, as shown in FIG. 39, the dispensing tip 50 is accommodated in the dispensing tip accommodating portion 15c by moving the syringe portion 151 in the Z1 direction (downward). Specifically, the syringe part 151 is moved in the Z1 direction (downward), and a dispensing tip releasing hole 161b (see FIG. 24) of the detaching member 161 is formed above the edge 151i of the cap 151b of the moved syringe part 151. The removing member 161 is moved in the X2 direction so as to be positioned. Further, in this state, by moving the syringe portion 151 in the Z2 direction (upward), the dispensing tip 50 presses the lower portion of the edge portion 151i of the cap 151b. A force in the direction (downward) (direction in which the dispensing tip 50 is removed from the nozzle portion 151a) is applied, and the dispensing tip 50 is detached from the nozzle portion 151a.

  Next, as shown in FIG. 40, the horizontal position (X direction) of the mounting portion 141 a of the holding member 141 matches the horizontal position of the filter tool storage chamber 11 in which the filter tool 40 is stored. Then, after moving the installation portion 131 in the X1 direction, the holding member 141 is moved in the Z1 direction (downward). At this time, the notch 40f (see FIG. 14) of the filter 40 is engaged with the protrusion 141j of the mounting portion 141a, and the engaging claw 40g (FIGS. 12 and 14) is the groove 141k (FIG. 20) of the mounting portion 141a. The filter 40 is press-fitted and attached to the attachment part 141a. Thereafter, by moving the holding member 141 in the Z2 direction (upward), the state shown in FIG. 41 is obtained.

  Then, as shown in FIG. 42, the horizontal position (X direction) of the mounting portion 141a to which the filtering tool 40 is mounted, and the horizontal position of the filtration processing storage chamber 14 in which the diluted tissue solution is stored. Is moved in the X1 direction so that. And the tissue solution after dilution is filtered in the storage chamber 14 for filtration by moving the holding member 141 in the Z1 direction (downward). Specifically, first, as shown in FIG. 43, the filter 40 is inserted into the filtration processing chamber 14 by moving the holding member 141 in the Z1 direction (downward). At this time, the opening cross-sectional area of the filtration processing chamber 14 is formed so as to gradually decrease from the opening portion 14a side toward the bottom surface portion 14b side. It is possible to suppress leakage of the diluted tissue solution from the gap between the two. Then, as shown in FIG. 44, the diluted tissue solution accommodated between the filtration tool 40 and the filtration treatment storage chamber 14 passes through two filters 41 and 42 to form a cylindrical filtration tool. 40 is extracted inside.

  45, the detaching member 161 is moved in the X2 direction so that the release claw 161a of the detaching member 161 is positioned above the contact portion 141m (see FIG. 19) of the holding member 141. Further, in this state, by moving the holding member 141 in the Z2 direction (upward), the flange part 40d (see FIG. 12) of the filter tool 40 presses the lower part of the contact part 141m. A force in the Z1 direction (downward) (a direction in which the filter 40 is removed from the mounting part 141a) is applied to the part 40d, the filter 40 is detached from the mounting part 141a, and the filter 40 is filtered. It will be in the state where it fits in storage chamber 14 for processing.

  Next, the position in the horizontal direction (X direction) of the nozzle portion 151a of the syringe portion 151 and the position in the horizontal direction of the dispensing tip storage portion 15d of the dispensing tip storage chamber 15 in which the dispensing tips 60 are stored are the same. As described above, the installation unit 131 is moved in the X2 direction, and the syringe unit 151 is moved in the Z1 direction (downward). At this time, the opening 60a (see FIG. 3) of the dispensing tip 60 is press-fitted into the tip portion 151h (see FIG. 22) of the nozzle portion 151a, and the dispensing tip 60 is attached to the nozzle portion 151a. Thereafter, the syringe unit 151 is moved in the Z2 direction (upward), the position in the horizontal direction (X direction) of the nozzle unit 151a to which the dispensing tip 60 is mounted, and the filtration processing storage chamber 14 in which the filtrate is stored. The state shown in FIG. 46 is obtained by moving the installation portion 131 in the X2 direction so that the horizontal position matches the horizontal position.

  And after moving the syringe part 151 to a Z1 direction (downward), as shown in FIG. 47, the filtrate in the storage chamber 14 for filtration is sucked. Then, the dispensing tip 60 is pulled up from the filtrate by moving the nozzle portion 151a to which the dispensing tip 60 is attached in the Z2 direction (upward). Then, the removal member 161 is moved in the X2 direction to a position below the pulled dispensing tip 60. Then, as shown in FIG. 48, the horizontal direction (X direction) position of the nozzle part 151a to which the dispensing tip 60 is attached and the horizontal direction of the collection container 300 installed in the collection container installation part 131b of the installation part 131 After the installation part 131 is moved in the X1 direction so that the positions of the removal member 161 coincide with each other, the removal member 161 is moved in the X1 direction.

  Thereafter, as shown in FIG. 49, the syringe unit 151 is moved in the Z1 direction (downward), and the filtrate sucked in the filtration processing storage chamber 14 is discharged in the collection container 300. As a result, the filtrate obtained from the lymph node 200 can be collected in the collection container 300.

  And while moving the nozzle part 151a with which the dispensing tip 60 was mounted | worn to Z2 direction (upward), the removal member 161 is moved to the position below the pulled-up dispensing tip 60 in X2 direction. And the horizontal position (X direction) of the nozzle part 151a to which the dispensing tip 60 is mounted and the horizontal position of the dispensing tip storage part 15d of the dispensing tip storage chamber 15 are set to coincide. The part 131 is moved in the X2 direction, and the detaching member 161 is moved in the X1 direction.

  Then, as shown in FIG. 50, the dispensing tip 50 is accommodated in the dispensing tip accommodating portion 15c by moving the syringe portion 151 in the Z1 direction (downward). Thereafter, the dispensing tip 60 is detached from the nozzle portion 151a in the same procedure as the dispensing tip 50 is detached.

  As described above, the filtrate collecting operation of the sample processing apparatus 100 is performed.

  In the present embodiment, as described above, the cylindrical filter 40 having the opening 40e on the upper side and the filters 41 and 42 on the lower side is held by the holding member 141 of the sample processing mechanism unit 140, By moving the held filtering tool 40 using the moving mechanism 142, the filtering tool 40 is stored in the filtration processing storage chamber 14 of the sample preparation container 10 in which the tissue solution obtained by crushing the lymph nodes is stored. , The tissue solution accommodated in the filtration treatment chamber 14 can be filtered by the filtration tool 40 having the opening 40e on the upper side. In this case, the dispensing tip 60 can be inserted into the inside of the filtering tool 40 through the opening 40e on the upper side of the filtering tool 40 by configuring the filtrate to be extracted inside the filtering tool 40. Therefore, the filtrate can be sucked by the dispensing tip 60. As a result, the extracted filtrate can be collected by filtering the tissue solution obtained by crushing the lymph nodes.

  In the present embodiment, as described above, the suction dispensing mechanism unit 150 (dispensing tip) is provided by providing the suction dispensing mechanism unit 150 to which the dispensing tip 60 for sucking and dispensing the filtrate is attached. 60), the extracted filtrate can be sucked through the opening 40e of the filter 40, and the sucked filtrate can be dispensed into the collection container 300. Thereby, a filtrate can be extract | collected easily with the suction dispensing mechanism part 150 (dispensing chip | tip 60).

  In the present embodiment, as described above, the suction dispensing mechanism unit 150 sucks the tissue solution adjusted in the crushing processing chamber 13 of the sample preparation container 10 and prepares the sucked tissue solution as a sample. The tissue solution prepared in the crushing storage chamber 13 is accommodated for filtration processing by being configured so that a dispensing tip 50 for dispensing can be attached to the filtration processing chamber 14 of the container 10. Since there is no need to separately provide a mechanism for transporting the chamber 14, the number of parts can be reduced and the apparatus can be downsized.

  In the present embodiment, as described above, the holding member 141 of the sample processing mechanism unit 140 is configured so as to be able to hold the crushing tool 30 for crushing the lymph node. When preparing the tissue solution by crushing, while holding the crushing tool 30 by the holding member 141, and when filtering the tissue solution, if the filtering tool 40 is held by the holding member 141, preparation of the tissue solution and Filtration can be performed in one apparatus. Further, when preparing and filtering the tissue solution in one apparatus, it is not necessary to separately provide a mechanism for holding the crushing tool 30 and inserting the crushing tool 30 into the crushing processing chamber 13. Therefore, this can also reduce the number of parts and reduce the size of the apparatus.

  Further, in the present embodiment, as described above, the holding member 141 is disposed above the sample preparation container 10 by configuring the holding member 141 to be movable in the vertical direction by the moving mechanism unit 142. In this case, if the holding member 141 is moved downward by the moving mechanism 142, the lymph node can be easily crushed and the tissue solution can be adjusted, and the tissue solution can be filtered. it can.

  Further, in the present embodiment, as described above, the holding member 141 of the sample processing mechanism unit 140 is provided with the press-fitting portion 141i into which the filtering tool 40 and the crushing tool 30 are press-fitted, so that the filtering tool 40 and the press-fitting portion 141i are provided. If the crushing tool 30 is press-fitted, the filter 40 and the crushing tool 30 can be easily held by the holding member 141. In this case, if the filtering tool 40 and the crushing tool 30 before use are accommodated in the sample preparation container 10, the holding member 141 can be easily press-fitted by moving the holding member 141 downward by the moving mechanism unit 142. The filter tool 40 and the crushing tool 30 before use accommodated in the sample preparation container 10 can be press-fitted and held in the part 141i.

  In the present embodiment, as described above, the removing mechanism 160 for removing at least one of the filtering tool 40 and the crushing tool 30 held by the holding member 141 of the sample processing mechanism 140 from the holding member 141 is provided. By this, when shifting from the preparation process of the tissue solution to the filtration process of the tissue solution, the crushing tool 30 can be detached from the holding member 141 by the removal mechanism unit 160 and the filtration tool 40 can be attached, and the filtration of the tissue solution can be performed. When shifting from the process to the preparation process of the tissue solution, the crushing tool 30 can be mounted by removing the filtering tool 40 from the holding member 141 by the removal mechanism 160.

  Further, in the present embodiment, as described above, the holding member 141 is moved upward by the moving mechanism unit 142 in a state where the removing member 161 is moved in the horizontal direction by the moving mechanism unit 162 and arranged at the removing position. Therefore, the holding member 141 for holding the filtering tool 40 and the crushing tool 30 can be moved only in the vertical direction by removing at least one of the filtering tool 40 and the crushing tool 30 from the holding member 141. Even if it does, at least one of the filter 40 and the crushing tool 30 can be easily removed from the holding member 141 by the movement of the holding member 141 in the vertical direction and the movement of the removal member 161 in the horizontal direction.

  In the present embodiment, as described above, the holding member 131 is provided by installing the installation unit 131 for installing the sample preparation container 10 and the moving mechanism unit 132 for moving the installation unit 131 in the horizontal direction. Even when 141 is configured to be movable only in the vertical direction, if the installation unit 131 to which the sample preparation container 10 is attached is moved in the horizontal direction by the moving mechanism unit 132, the filtration tool is used when the tissue solution is filtered. The horizontal position of the holding member 141 holding 40 and the filtration processing chamber 14 of the sample preparation container 10 can be matched, and the crushing tool 30 is held when preparing the tissue solution. The horizontal positions of the holding member 141 and the crushing storage chamber 13 of the sample preparation container 10 can be matched. As a result, even if the holding member 141 is configured to be movable only in the vertical direction, the filter 40 can be inserted into the filtration processing chamber 14 of the sample preparation container 10 when the tissue solution is filtered. At the same time, when preparing the tissue solution, the crushing tool 30 can be inserted into the crushing storage chamber 13 of the sample preparation container 10.

  The embodiment disclosed this time should be considered as illustrative in all points and not restrictive. The scope of the present invention is shown not by the above description of the embodiments but by the scope of claims for patent, and further includes all modifications within the meaning and scope equivalent to the scope of claims for patent.

  For example, in the above-described embodiment, the example in which the present invention is applied to the sample processing apparatus capable of preparing the tissue solution and filtering the tissue solution has been described. However, the present invention is not limited thereto, and the tissue solution is not limited thereto. It can also be applied to a sample processing apparatus that performs only filtration.

  Moreover, in the said embodiment, although the holding member which can hold | maintain both a crushing tool and a filter tool was used, this invention is not restricted to this, The holding member for holding a crushing tool, and a filter tool are used. You may provide separately the holding member for hold | maintaining.

  Further, in the above-described embodiment, the collection of the filtrate and the transport of the tissue solution from the crushing storage chamber to the filtration processing chamber are performed by one suction dispensing mechanism unit. Not limited to this, a suction dispensing mechanism for collecting the filtrate and a suction dispensing mechanism for transporting the tissue solution from the crushing storage chamber to the filtration processing chamber are provided separately. May be.

  Moreover, in the said embodiment, a crushing tool is inserted from the upper part into the crushing process storage chamber in which the lymph node and crushing solution were accommodated, and the crushing tool is moved up and down to make the lymph node a predetermined size. However, the present invention is not limited to this, and the present invention is not limited to this. For the crushing treatment in which the lymph node and the crushing solution are accommodated, the inner crushing member of the crushing tool is rotated. You may make it refine | miniaturize a lymph node by inserting the crushing tool into a storage chamber from the upper part, and rotating the inner crushing member of a crushing tool, moving the crushing tool up and down.

  In the above embodiment, only one collection container is installed and the filtrate filtered in the filtration processing chamber is sucked by the dispensing tip and then discharged into one collection container. The invention is not limited to this, and two collection containers are installed, and the diluting solution stored in the filtration processing chamber is pre-dispensed into one collection container by a dispensing tip, A diluted sample may be prepared by sucking the discharged filtrate with a dispensing tip and then discharging the filtrate into one collection container.

It is the perspective view which showed the whole structure of the sample processing apparatus by one Embodiment of this invention. FIG. 2 is a perspective view of a material preparation kit attached to the sample processing apparatus according to the embodiment shown in FIG. 1. It is an exploded view of the sample preparation kit with which the sample processing apparatus by one Embodiment shown in FIG. 1 is mounted | worn. It is the perspective view which showed the state which removed the cover part of the sample preparation kit with which the sample processing apparatus by one Embodiment shown in FIG. 1 was mounted | worn. It is the perspective view which showed the sample preparation container of the sample preparation kit with which the sample processing apparatus by one Embodiment shown in FIG. 1 was mounted | worn. It is the top view which showed the sample preparation container of the sample preparation kit with which the sample processing apparatus by one Embodiment shown in FIG. 1 is mounted | worn. It is the enlarged front view which showed the convex part of the solution storage chamber for crushing of the sample preparation container of the sample preparation kit with which the sample processing apparatus by one Embodiment shown in FIG. 1 is mounted | worn. It is the enlarged plan view which showed the convex part of the solution storage chamber for crushing of the sample preparation container of the sample preparation kit with which the sample processing apparatus by one Embodiment shown in FIG. 1 is mounted | worn. It is an exploded view of the crushing tool of the sample preparation kit with which the sample processing apparatus according to the embodiment shown in FIG. 1 is mounted. It is a front view of the inner side crushing member of the crushing tool of the sample preparation kit with which the sample processing apparatus by one Embodiment shown in FIG. 1 is mounted | worn. It is a top view of the outer side crushing member of the crushing tool of the sample preparation kit with which the sample processing apparatus by one Embodiment shown in FIG. 1 is mounted | worn. It is a front view of the filter of the sample preparation kit with which the sample processing apparatus by one Embodiment shown in FIG. 1 is mounted | worn. It is sectional drawing of the filter of the sample preparation kit with which the sample processing apparatus by one Embodiment shown in FIG. 1 is mounted | worn. It is a top view of the filter of the sample preparation kit with which the sample processing apparatus by one Embodiment shown in FIG. 1 is mounted | worn. It is a perspective view of the main-body part of the sample processing apparatus by one Embodiment shown in FIG. It is a top view of the main-body part of the sample processing apparatus by one Embodiment shown in FIG. It is the perspective view which showed the state at the time of installing a sample preparation kit in the installation part of the sample processing apparatus by one Embodiment shown in FIG. It is a top view of the installation part of the sample processing apparatus by one Embodiment shown in FIG. It is a perspective view of the holding | maintenance part of the sample processing apparatus by one Embodiment shown in FIG. It is the perspective view which showed the state at the time of mounting | wearing the crushing tool of a sample preparation kit with the holding | maintenance part of the sample processing apparatus by one Embodiment shown in FIG. It is a front view of the syringe part of the sample processing apparatus by one Embodiment shown in FIG. It is the front view which showed the state at the time of mounting | wearing the dispensing tip of a sample preparation kit with the nozzle part of the syringe part of the sample processing apparatus by one Embodiment shown in FIG. It is a perspective view of the removal mechanism part of the sample processing apparatus by one Embodiment shown in FIG. It is a top view of the removal member of the removal mechanism part of the sample processing apparatus by one Embodiment shown in FIG. It is a figure for demonstrating the sampling operation | movement of the filtrate of the sample processing apparatus by one Embodiment of this invention. It is a figure for demonstrating the sampling operation | movement of the filtrate of the sample processing apparatus by one Embodiment of this invention. It is a figure for demonstrating the sampling operation | movement of the filtrate of the sample processing apparatus by one Embodiment of this invention. It is sectional drawing for demonstrating the operation | movement which the crushing tool of the sample preparation kit shown in FIG. 2 crushes a lymph node. It is sectional drawing for demonstrating the operation | movement which the crushing tool of the sample preparation kit shown in FIG. 2 crushes a lymph node. It is sectional drawing for demonstrating the operation | movement which the crushing tool of the sample preparation kit shown in FIG. 2 crushes a lymph node. It is a figure for demonstrating the sampling operation | movement of the filtrate of the sample processing apparatus by one Embodiment of this invention. It is a figure for demonstrating the sampling operation | movement of the filtrate of the sample processing apparatus by one Embodiment of this invention. It is a figure for demonstrating the sampling operation | movement of the filtrate of the sample processing apparatus by one Embodiment of this invention. It is a figure for demonstrating the sampling operation | movement of the filtrate of the sample processing apparatus by one Embodiment of this invention. It is a figure for demonstrating the sampling operation | movement of the filtrate of the sample processing apparatus by one Embodiment of this invention. It is a figure for demonstrating the sampling operation | movement of the filtrate of the sample processing apparatus by one Embodiment of this invention. It is a figure for demonstrating the sampling operation | movement of the filtrate of the sample processing apparatus by one Embodiment of this invention. It is a figure for demonstrating the sampling operation | movement of the filtrate of the sample processing apparatus by one Embodiment of this invention. It is a figure for demonstrating the sampling operation | movement of the filtrate of the sample processing apparatus by one Embodiment of this invention. It is a figure for demonstrating the sampling operation | movement of the filtrate of the sample processing apparatus by one Embodiment of this invention. It is a figure for demonstrating the sampling operation | movement of the filtrate of the sample processing apparatus by one Embodiment of this invention. It is a figure for demonstrating the sampling operation | movement of the filtrate of the sample processing apparatus by one Embodiment of this invention. It is a figure for demonstrating the sampling operation | movement of the filtrate of the sample processing apparatus by one Embodiment of this invention. It is sectional drawing which showed the state from which a filtrate is extracted with the filter of the sample preparation kit shown in FIG. It is a figure for demonstrating the sampling operation | movement of the filtrate of the sample processing apparatus by one Embodiment of this invention. It is a figure for demonstrating the sampling operation | movement of the filtrate of the sample processing apparatus by one Embodiment of this invention. It is a figure for demonstrating the sampling operation | movement of the filtrate of the sample processing apparatus by one Embodiment of this invention. It is a figure for demonstrating the sampling operation | movement of the filtrate of the sample processing apparatus by one Embodiment of this invention. It is a figure for demonstrating the sampling operation | movement of the filtrate of the sample processing apparatus by one Embodiment of this invention. It is a figure for demonstrating the sampling operation | movement of the filtrate of the sample processing apparatus by one Embodiment of this invention.

Explanation of symbols

10 Sample preparation container (container)
13 Crushing chamber (crushing chamber)
14 Filtering chamber (filtration chamber)
30 crushing tool 40 filtering tool 40e opening 41, 42 filter 131 installation part (container mounting member)
132 Movement mechanism part (third movement mechanism part)
141 holding member (crushing tool holding member, filter holding member)
141i Press-fit part 142 Movement mechanism part (1st movement mechanism part, crushing tool movement mechanism part, filter movement mechanism part)
150 Suction Dispensing Mechanism Unit 160 Removal Mechanism Unit 161 Removal Member 162 Movement Mechanism Unit (Second Movement Mechanism Unit)
300 Collection container

Claims (9)

Container for accommodating the biological tissue, crushing device for crushing the body tissue, and, to a sample processing apparatus for processing a sample using a filtration device for filtering a tissue solution obtained by crushing the body tissue,
The storage container stores a bottomed filtration chamber provided with an opening, a bottomed crushing chamber provided with an opening, a crushing tool storage chamber for storing a crushing tool before use, and a filtering tool before use. A filter storage chamber, and adjacent to each other in a row,
The crushing tool has an end for crushing a living tissue,
The filter has a cylindrical body provided with an opening at one end and a filter at the other end,
The sample processing apparatus includes:
A container platform capable of mounting the container,
A rotatable holding member at least one of the holding and retained the crushing member of the crushing tool and the filtration device,
A first moving mechanism that enables the holding member to move in the vertical direction ;
A suction dispensing mechanism that is movable in the vertical direction and at least dispenses the tissue solution by suction;
A removal mechanism for removing at least one of the filtering tool and the crushing tool held by the holding member from the holding member ,
The container mounting part is movable in a horizontal direction with respect to the holding member and the suction dispensing mechanism part,
By moving the holding member by the first moving mechanism unit, the crushing tool stored in the storage container is held by the holding member,
By moving the crushing tool held by the holding member by the first moving mechanism part, the crushing tool is inserted from the end through the opening of the crushing chamber of the storage container placed on the container placing part . In addition, by rotating the crushing tool held by the holding member, to prepare a tissue solution by crushing the biological tissue,
The suction dispensing mechanism section sucks the tissue solution from the crushing chamber of the storage container and dispenses the sucked tissue solution into a filtration chamber provided separately from the crushing chamber of the storage container.
The crushing tool held by the holding member is removed from the holding member by the removing mechanism,
By moving the holding member by the first moving mechanism unit, the holding member is held by the holding member in the filter container,
By moving the filter held by the holding member by the first moving mechanism, the filter is inserted from the other end through the opening of the filtration chamber of the container, and the tissue solution is filtered to remove the filtrate. Sample processing equipment to extract.   The sample processing apparatus according to claim 1, wherein the filtrate is extracted into a cylindrical body constituting the filter. The container mounting part is further configured to be capable of mounting a collection container that contains the extracted filtrate,
The sample processing apparatus according to claim 1, wherein the suction / dispensing mechanism section has a function of sucking the filtrate extracted by a filter and dispensing the sucked filtrate into a collection container. The holding member is disposed above the storage container placed on the container placement part ,
While holding the crushing tool by pre Symbol holding member by inserting the crushing tool to the crushing chamber of the is allowed by container moving said holding member by the first moving mechanism downward, breaking the living tissue lines I,
With the filter held by the holding member, the tissue solution is filtered by moving the holding member downward by the first moving mechanism and inserting the filter into the filtration chamber of the storage container. The sample processing apparatus of any one of Claims 1-3.   The sample processing apparatus according to claim 1, wherein the holding member includes a press-fitting portion into which a filtering tool and a crushing tool are press-fitted. By moving the front Kiho support member downward by the pre-Symbol first moving mechanism, filtration equipment and crushing device which is accommodated in the placed container to the container mounting part is press-fit portion of the retaining member The sample processing apparatus according to claim 5, wherein the sample processing apparatus is press-fitted into the apparatus. The detaching mechanism portion, at least one of filtration equipment and crushing device which is press-fitted into the press-fitting portion of the holding member is removed from the holding member, the sample processing apparatus according to claim 5 or 6. The removal mechanism section is a removal member for removing the filtering tool and the crushing tool from the holding member,
A second moving mechanism for disposing the removing member in a removing position in the vicinity of the press-fitting portion of the holding member by moving the removing member in a horizontal direction;
A filter is removed from the holding member by moving the holding member upward by the first moving mechanism in a state where the removing member is moved in the horizontal direction by the second moving mechanism and arranged at the removing position. The sample processing apparatus according to claim 7, wherein at least one of the crushing tool and the crushing tool is removed.   A first position at which a horizontal position of the holding member on which the filter is held and a filtration chamber of a storage container placed on the container placing portion substantially match the container placing portion; A third for moving at least to a second position where the horizontal positions of the holding member holding the crushing tool and the crushing chamber of the receiving container placed on the container placing portion substantially coincide with each other. The sample processing apparatus according to claim 1, further comprising a moving mechanism unit.

Images