CN1940055B - Sample preparation kit, sample preparation container, and sample processing device - Google Patents

Sample preparation kit, sample preparation container, and sample processing device Download PDF

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Publication number
CN1940055B
CN1940055B CN2006101410600A CN200610141060A CN1940055B CN 1940055 B CN1940055 B CN 1940055B CN 2006101410600 A CN2006101410600 A CN 2006101410600A CN 200610141060 A CN200610141060 A CN 200610141060A CN 1940055 B CN1940055 B CN 1940055B
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broken
chamber
filters
aforementioned
appliance
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CN1940055A (en
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正见圭一郎
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Sysmex Corp
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Sysmex Corp
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Priority claimed from JP2005283183A external-priority patent/JP4795765B2/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting

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  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
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  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention provides a sample-preparing vessel including a first storing chamber provided with a first opening at one terminal, and a protrusion part formed on the inner bottom face of a first bottom part at the other terminal, wherein a pulverizing treatment to biological organism can be carried out by a knapper. The invention also provides a sample-preparing suite including a sample-preparing vessel and pulverized fluid used for pulverizing biological organism stored in first storing chamber of the sample-preparing vessel. In addition, a sample-treatment device is provided, including fixing member for protrusion part, transfer device for filter, suction moved distribution loading device and etc. The device provided by this invention makes the process of preparing and treating of biological sample more simple and easy, while the sample quality can be insured without a lot of fussy steps.

Description

Specimen preparation container and kit, sample preparation and apparatus for samples treatment
Technical field:
The present invention relates to specimen preparation container and kit, sample preparation, particularly relate to specimen preparation container and kit, sample preparation with the harvesting chamber that is used for broken biological tissue.The invention still further relates to apparatus for samples treatment, particularly handle the apparatus for samples treatment of samples such as biological tissue.
Background technology:
Sample processing unit (plant) before known is in a bottom is the thin concavity container that (bottom surface sections) is gradually sharp downwards; Processome uneven with a surface irregularity, the pointed convex of top likeness in form container bottom rotates back and forth, and smalls (example can with reference to patent documentation 1) is processed in biological tissue in the container.This sample processing unit (plant) is similar with the concavity interior shape of container bottom because of the most advanced and sophisticated convex of its processome, so the rotation processome, the rill of its uneven surface just cuts off biological tissue, and this biological tissue is pulverized.
Yet; Drive disclosed sample processing unit (plant) on the clear 63-112974 communique according to the spy; Although the inboard concavity shape of the front end convex of processome and container is similar, on working accuracy, be difficult to make inner the coincideing of the vertical convex of processome concavity complete and container taper bottom.Therefore; Sometimes between the concavity inboard that the convex head and the container of processome are carefully held, have inaccessible space, processome top (wasted space); Consequently the convex head of processome is difficult to arrive and is deep into container bottom spatial biological tissue, thereby is difficult to fully broken this biological tissue sometimes.
General apparatus for samples treatment (sample processing unit (plant)) all can be implemented brokenly in the broken liquid of special broken biological tissue in container during broken biological tissue, prepares sample with this.Drive disclosed sample processing unit (plant) on the clear 63-112974 communique according to the spy; Want the biological tissue in the broken container; After must the container of biological tissue being housed from the broken liquid injection that a specific place obtains, again processome is put into this container, the work of the broken biological tissue of result becomes very loaded down with trivial details.
In addition; Though the special sample processing unit (plant) of opening on the clear 63-112974 communique is not open; But want the broken biological tissue that is contained in the container usually; Must get earlier each broken biological tissue from specific place and all will carry out the broken appliance that heat kill bacterium etc. is handled, this broken appliance installed to again and support and make on its rotary equipment, the work of broken biological tissue is very loaded down with trivial details.
In addition drive the sample processing unit (plant) on the clear 63-112974 communique according to the spy, not have open how extraction filtrating from the sample that broken biological tissue processes, but after the sample that usually broken biological tissue is obtained divides appearance, filter with the sample that divides appearance, extraction is filtrated.For this reason, the sample processing unit (plant) that the spy opens clear 63-112974 communique will inject respectively after the sample that broken biological tissue is obtained divides appearance and can carry out filtering filtration unit in order to extract filtrating, and it is very inconvenient therefore to filter the sample work that obtains from biological tissue.
Always as the apparatus for samples treatment of handling samples such as biological tissue, known having contains the filtration unit of filters and contains (such as please opening flat 6-221976 communique and Te Kai 2000-333669 communique with reference to the spy) such as homogenizers (device that sample is homogenized) of broken appliance.
The US5282978 communique discloses the filtration unit of making the sample slide glass; Filtration contains the tissue juice (suspension liquid) of the cell particle that is obtained by broken biological tissue; Only take cell particle, make cell particle greater than institute's dimensioning attached to the surface greater than institute's dimensioning.Filters on this US5282978 communique is an end closure, and the other end is equipped with the tubular article of filter paper.According to above-mentioned US5282978 communique, when making the sample slide glass, at first filters is inserted and be equipped with in the container of tissue juice, in filters, form negative pressure again, extracting interstitial fluid is extracted into filtrating in the filters.At this moment, see through filter paper less than the cell particle of institute's dimensioning and move on in the filters, then do not stay the filter paper appearance through filter paper greater than the cell particle of institute's sizing.Let the filter paper appearance of filters contact, make the cell particle that remains in the filter paper appearance attached to slide surface with slide surface.Like this, the sample slide glass is just made and is finished.
Drive disclosed homogenizer on the 2000-333669 communique according to above-mentioned spy, with broken appliance (pestle) biological tissue is carried out homogenizing and handle, making contains by the tissue juice of the biological tissue of homogenizing.This spy opens the homogenizing that disclosed homogenizer on the 2000-333669 communique only carries out biological tissue.
Yet the filtration unit on the US5282978 communique also has a problem to be, it is the tubular design that an end closure, the other end are equipped with filter paper, and filtrating is pumped in the filters, and sampling was had any problem when filtrating was analyzed.
The homogenizer that above-mentioned spy opens the 2000-333669 communique also exists the equipment that does not filter tissue juice, the problem that need prepare filtration unit when filtering tissue juice separately.
Summary of the invention:
The object of the invention is and solves above-mentioned problem.One of the object of the invention is to provide the specimen preparation container and the kit, sample preparation of the practical broken biological tissue of available broken appliance.
Two of the object of the invention is to provide the kit, sample preparation that can simplify the work of broken biological tissue.
Three of the object of the invention is to provide the kit, sample preparation of the filtration work that can simplify the tissue juice that obtains from biological tissue.
Four of the object of the invention is to provide a kind of apparatus for samples treatment, and it can extract through filtering the filtrating of extraction in the tissue juice that the broken biological tissue of filtration obtains.
Specimen preparation container provided by the present invention, its structure is following: an end has first opening, and the other end has the first protruding bottom of bottom inside, and also useful the past is stated the first harvesting chamber of the broken biological tissue of broken appliance of first opening insertion.
The convexity of first bottom of the wherein said first harvesting chamber has identical radius-of-curvature with the vertical recess of said broken appliance.The convexity of first bottom of the said first harvesting chamber is the ribbed arch shape in said first bottom; When the vertical recess of said broken appliance rotates with respect to said ribbed arch shape is protruding, form the gap between the part beyond the ribbed arch shape convexity of first bottom of vertical recess of said broken appliance and the said first harvesting chamber.
Wherein also be provided with the second harvesting chamber, be used to receive and keep the tissue juice that broken biological tissue obtains in the said first harvesting chamber, filter the tissue juice of aforementioned harvesting simultaneously.The wherein said second harvesting chamber one end is provided with second opening, and the other end is provided with second bottom, its opening part sectional area from said second opening towards the said second bottom convergent.The wherein said first harvesting chamber can with the said second harvesting chamber one.
Kit, sample preparation provided by the present invention, it comprises: the specimen preparation container: this container one end has first opening, and also useful the past is stated the first harvesting chamber of the broken biological tissue of broken appliance of first opening insertion simultaneously; And harvesting in the aforementioned specimen preparation container first harvesting chamber, be used for the broken liquid of broken biological tissue.
Wherein said specimen preparation container also contains the second harvesting chamber; Be used to receive and keep the tissue juice that broken biological tissue obtains in the said first harvesting chamber; Filter the tissue juice of aforementioned harvesting simultaneously; Also have diluent, harvesting is used to dilute the tissue juice that aforementioned broken biological tissue obtains in the said second harvesting chamber.
Wherein said specimen preparation container is provided with the 3rd of the broken appliance of taking in broken biological tissue fully
The harvesting chamber also is provided with the indoor broken appliance of the 3rd harvesting that is accommodated in said specimen preparation container.
Wherein said broken appliance comprises interior crushing members and can crushing members in aforementioned be taken in outer crushing members wherein that aforementioned interior crushing members can be with respect to aforementioned outer crushing members rotation.Wherein said outer crushing members is provided with slit near the top.
Wherein said specimen preparation container contains the 4th harvesting chamber of taking in the filters that filters broken biological tissue gained tissue juice, and is accommodated in the indoor filters of said specimen preparation container the 4th harvesting.
Wherein said specimen preparation container is provided with to deposit and is used to inhale the 5th harvesting chamber of moving, extract the packing head that filters the filtrating that tissue juice obtains, also is provided with the packing head that leaves said specimen preparation container the 5th harvesting chamber in.
Wherein also be provided with the lid that can embed said specimen preparation container.
A kind of kit, sample preparation provided by the present invention comprises:
The break process chamber, an end is provided with opening, and broken appliance inserts and then broken biological tissue from aforementioned opening;
The specimen preparation container, it is provided with the broken appliance chamber of the broken appliance that is used to deposit broken aforementioned biological tissue; And the aforementioned broken appliance that leaves aforementioned broken appliance chamber in.
Another kind of kit, sample preparation provided by the present invention comprises:
The filtration treatment chamber is used to accept and filter the tissue juice that broken biological tissue obtains;
The specimen preparation container is useful on the filters chamber of depositing the filters that filters aforementioned biological tissue; And the filters that leaves aforementioned filters chamber in.
Apparatus for samples treatment provided by the present invention comprises:
Fixer, it is used at least fixing being provided with the filters that cylindrical shell that opening, the other end be provided with filter paper constitutes by an end; And first mobile equipment that is used for moving the aforementioned fixation device,
The filtration chamber; It is arranged in housing container and is used to filter broken biological tissue gained tissue juice; The aforementioned filters that wherein aforementioned first mobile equipment will be fixed on the aforementioned fixation device inserts in the aforementioned filtration chamber; Filter aforementioned tissue juice, extraction filtrating is inhaled the aforementioned filtrating of moving extraction through aforementioned filters opening.
Wherein said filtrating is extracted to the barrel of said filters.Wherein also be provided with at least to inhale and move the suction of extracting said filtrating and move dispensing apparatus, and sampling container, it is used for ccontainingly moving dispensing apparatus by suction and inhaling the said filtrating of moving the extraction gained that injects the back through the opening of said filters.
Wherein aforementioned housing container also is provided with the crushing chamber that broken aforementioned biological tissue prepares aforementioned tissue juice except that said filtration chamber, wherein said suction moves dispensing apparatus the aforementioned tissue juice that suction moves is injected aforementioned housing container.Wherein the aforementioned fixation device is fixed the broken appliance of broken aforementioned biological tissue; Aforementioned first mobile equipment moves the aforementioned broken appliance that is fixed on the aforementioned fixation device; Be inserted in the crushing chamber of aforementioned housing container, and broken aforementioned biological tissue, prepare aforementioned tissue juice.
Wherein said fixer is configured in the place that is higher than said housing container; Said first mobile equipment can move up and down said fixer; When said fixer secures the above broken appliance; Move down said fixer with said first mobile equipment, said broken appliance is inserted the crushing chamber of said housing container, carry out the fragmentation of said biological tissue; When said fixer secures the above filters, move down said fixer with said first mobile equipment, the filtration chamber with said filters inserts said housing container carries out the filtration of said biological tissue.
Wherein said fixer contains the pressing part that can be pressed into said strainer and said broken appliance.
Wherein untapped said strainer and said broken appliance leave in the aforementioned housing container; Move down said fixer with said first mobile equipment, the pressing part that the said strainer and the said broken appliance that leave in the aforementioned housing container are pressed into said fixer.
Wherein also be provided with dismounting equipment, its be used for from the dismounting of said fixer be pressed into said fixer pressing part said filters and said broken appliance at least one of them.
Wherein said dismounting equipment is useful on the dismounting parts and second mobile equipment of dismantling said filters and said broken appliance from said fixer; This second mobile equipment is used to move horizontally aforementioned dismounting parts; Aforementioned dismounting parts are placed the removed position on said fixer pressing part next door
When aforementioned second mobile equipment moves horizontally aforementioned dismounting parts when the aforementioned removed position, the aforementioned first mobile equipment said fixer that moves up makes said filters and said broken appliance break away from one of at least said fixer.
Wherein be configured in said fixer below in addition, the housing container installation parts and the 3rd mobile equipment of said housing container are housed, the aforementioned fixation device that the 3rd mobile equipment is used for moving at least aforementioned housing container installation parts to fixing said filters and the first location of filtration chamber's horizontal alignment of aforementioned housing container and the second position of crushing chamber horizontal alignment of fixing aforementioned fixation device and the aforementioned housing container of said broken appliance.
Based on a kind of apparatus for samples treatment of the present invention, comprising:
The broken appliance fixer that is used for fixing the broken appliance of broken biological tissue; Move the broken appliance mobile equipment of aforementioned broken appliance fixer; Being used for fixing the broken aforementioned biological tissue of filtration gets
The filters fixer of the filters of the tissue juice that arrives; The filters mobile equipment of moving filter utensil fixer; At least inhale the suction that moves the aforementioned tissue juice of extraction and move dispensing apparatus; Aforementioned broken appliance mobile equipment moves the aforementioned broken appliance that is fixed on the aforementioned broken appliance fixer; With the crushing chamber of aforementioned broken appliance insertion housing container, broken aforementioned biological tissue prepares aforementioned tissue juice; Aforementioned suction moves dispensing apparatus and inhales from the crushing chamber of aforementioned housing container and move aforementioned tissue juice, and the aforementioned tissue juice that will extract injects the filtration chamber of aforementioned housing container; Aforementioned filters mobile equipment moves the aforementioned filters that is fixed on the aforementioned filters fixer, aforementioned filters is inserted the filtration chamber of housing container, and filter aforementioned tissue juice, extraction filtrating.
Wherein said broken appliance fixer and said filters fixer are made up of same fixer; Said broken appliance mobile equipment and said filters mobile equipment are made up of same mobile equipment.Wherein said suction moves the said filtrating that dispensing apparatus extracts said filters extraction, and this filtrating that will extract is injected sampling container.
Device provided by the present invention makes the preparation of biological material and processing thereof more succinctly make things convenient for, and has save a lot of loaded down with trivial details steps, has guaranteed the quality of sample simultaneously.
Description of drawings:
Fig. 1 is the entire infrastructure stereographic map of the apparatus for samples treatment of the kit, sample preparation of use an embodiment of the present invention.
Fig. 2 is the stereographic map of the kit, sample preparation of an embodiment of the present invention.
Fig. 3 is the exploded view of the kit, sample preparation of an embodiment shown in Figure 2.
Fig. 4 is the stereographic map after the kit, sample preparation of an embodiment shown in Figure 2 goes to cover.
Fig. 5 is the specimen preparation container stereographic map of the kit, sample preparation of an embodiment shown in Figure 2.
Fig. 6 is the specimen preparation container plat of the kit, sample preparation of an embodiment shown in Figure 2.
Fig. 7 is the protruding amplification front elevation of broken liquid chamber of specimen preparation container of the kit, sample preparation of an embodiment shown in Figure 2.
Fig. 8 is the protruding amplification view of broken liquid chamber of specimen preparation container of the kit, sample preparation of an embodiment shown in Figure 2.
Fig. 9 is the broken appliance exploded view of the kit, sample preparation of an embodiment shown in Figure 2.
Figure 10 is the interior crushing members front view of broken appliance of the kit, sample preparation of an embodiment shown in Figure 2.
Figure 11 is the outer crushing members plat of broken appliance of the kit, sample preparation of an embodiment shown in Figure 2.
Figure 12 is the filters front view of the kit, sample preparation of an embodiment shown in Figure 2.
Figure 13 is the sectional view of filters of the kit, sample preparation of an embodiment shown in Figure 2.
Figure 14 is the plat of filters of the kit, sample preparation of an embodiment shown in Figure 2.
Figure 15 is the stereographic map of the main body of apparatus for samples treatment shown in Figure 1.
Figure 16 is the plat of the main body of apparatus for samples treatment shown in Figure 1.
Figure 17 is for being installed in the kit, sample preparation of an embodiment shown in Figure 2 the stereographic map of the installation position of apparatus for samples treatment.
Figure 18 is the plat of the installation position of apparatus for samples treatment shown in Figure 1.
Figure 19 is the stereographic map of the support section of apparatus for samples treatment shown in Figure 1.
Figure 20 is installed to the stereographic map on the knife rest of support section for the broken appliance with the kit, sample preparation of an embodiment shown in Figure 2.
Figure 21 is the front view of the syringe of apparatus for samples treatment shown in Figure 1.
Figure 22 is the front view that the packing head of the kit, sample preparation of an embodiment shown in Figure 2 is installed to the syringe mouth.
Figure 23 is the stereographic map of the dismounting equipment of apparatus for samples treatment shown in Figure 1.
Figure 24 is the plat of dismounting parts of the dismounting equipment of apparatus for samples treatment shown in Figure 1.
Figure 25 is the synoptic diagram of the apparatus for samples treatment extraction filtrating process of the kit, sample preparation of use an embodiment of the present invention.
Figure 26 is the synoptic diagram of the apparatus for samples treatment extraction filtrating process of the kit, sample preparation of use an embodiment of the present invention.
Figure 27 is the synoptic diagram of the apparatus for samples treatment extraction filtrating process of the kit, sample preparation of use an embodiment of the present invention.
Figure 28 is the sectional view of the broken lymphoglandula process of broken appliance of the kit, sample preparation of a signal embodiment shown in Figure 2.
Figure 29 is the sectional view of the broken lymphoglandula process of broken appliance of the kit, sample preparation of a signal embodiment shown in Figure 2.
Figure 30 is the sectional view of the broken lymphoglandula process of broken appliance of the kit, sample preparation of a signal embodiment shown in Figure 2.
Figure 31 is the synoptic diagram of the apparatus for samples treatment extraction filtrating process of the kit, sample preparation of a use embodiment shown in Figure 2.
Figure 32 is the synoptic diagram of the apparatus for samples treatment extraction filtrating process of the kit, sample preparation of a use embodiment shown in Figure 2.
Figure 33 is the synoptic diagram of the apparatus for samples treatment extraction filtrating process of the kit, sample preparation of a use embodiment shown in Figure 2.
Figure 34 is the synoptic diagram of the apparatus for samples treatment extraction filtrating process of the kit, sample preparation of a use embodiment shown in Figure 2.
Figure 35 is the synoptic diagram of the apparatus for samples treatment extraction filtrating process of the kit, sample preparation of a use embodiment shown in Figure 2.
Figure 36 is the synoptic diagram of the apparatus for samples treatment extraction filtrating process of the kit, sample preparation of a use embodiment shown in Figure 2.
Figure 37 is the synoptic diagram of the apparatus for samples treatment extraction filtrating process of the kit, sample preparation of a use embodiment shown in Figure 2.
Figure 38 is the synoptic diagram of the apparatus for samples treatment extraction filtrating process of the kit, sample preparation of a use embodiment shown in Figure 2.
Figure 39 is the synoptic diagram of the apparatus for samples treatment extraction filtrating process of the kit, sample preparation of a use embodiment shown in Figure 2.
Figure 40 is the synoptic diagram of the apparatus for samples treatment extraction filtrating process of the kit, sample preparation of a use embodiment shown in Figure 2.
Figure 41 is the synoptic diagram of the apparatus for samples treatment extraction filtrating process of the kit, sample preparation of a use embodiment shown in Figure 2.
Figure 42 is the synoptic diagram of the apparatus for samples treatment extraction filtrating process of the kit, sample preparation of a use embodiment shown in Figure 2.
Figure 43 is the synoptic diagram of the apparatus for samples treatment extraction filtrating process of the kit, sample preparation of a use embodiment shown in Figure 2.
Figure 44 is the sectional view that the filters of the kit, sample preparation of an embodiment shown in Figure 2 extracts the filtrating situation.
Figure 45 is the synoptic diagram of the apparatus for samples treatment extraction filtrating process of the kit, sample preparation of a use embodiment shown in Figure 2.
Figure 46 is the synoptic diagram of the apparatus for samples treatment extraction filtrating process of the kit, sample preparation of a use embodiment shown in Figure 2.
Figure 47 is the synoptic diagram of the apparatus for samples treatment extraction filtrating process of the kit, sample preparation of a use embodiment shown in Figure 2.
Figure 48 is the synoptic diagram of the apparatus for samples treatment extraction filtrating process of the kit, sample preparation of a use embodiment shown in Figure 2.
Figure 49 is the synoptic diagram of the apparatus for samples treatment extraction filtrating process of the kit, sample preparation of a use embodiment shown in Figure 2.
Figure 50 is the synoptic diagram of the apparatus for samples treatment extraction filtrating process of the kit, sample preparation of a use embodiment shown in Figure 2.
Embodiment:
Below according to description of drawings embodiment of the present invention.
Kit, sample preparation provided by the present invention, it comprises: an end has opening, uses from the crushing chamber of the broken biological tissue of broken appliance that aforementioned opening inserts; Take in the broken appliance chamber of aforementioned broken biological tissue utensil; And harvesting is at the aforementioned broken appliance of aforementioned broken appliance chamber.
Kit, sample preparation provided by the present invention, it comprises: accept broken biological tissue and tissue juice and carry out filtering filtration chamber; The specimen preparation container that the filters chamber of taking in aforementioned filtration tissue juice utensil is arranged; And harvesting is at the aforementioned filters of aforementioned filters chamber.
Apparatus for samples treatment provided by the present invention, its structure is following: this device has filters fixer and first mobile equipment that is used for moving this fixer, its fixer be used to support an end opening,
The other end is equipped with the cartridge filter tool of filter paper.Aforementioned first mobile equipment moves the aforementioned filters that is supported by the aforementioned fixation device; Aforementioned filters is inserted the aforementioned filtration chamber that is used to filter the tissue juice that broken biological tissue obtains in the housing container; Filter aforementioned tissue juice; Extraction filtrating, the opening through aforementioned filters extracts aforementioned filtrating.
Apparatus for samples treatment provided by the present invention, its structure is following: this device be useful on the broken appliance that supports broken biological tissue the broken appliance fixer, move this broken appliance fixer the broken appliance mobile equipment, support the filters that is used to filter broken aforementioned biological tissue gained tissue juice the filters fixer, move the filters mobile equipment of aforementioned filters fixer and be used to inhale the suction that moves the aforementioned tissue juice of packing at least and move dispensing apparatus.The aforementioned broken appliance of aforementioned broken appliance mobile equipment mobile crushing utensil anchor support; Aforementioned broken appliance is inserted in the crushing chamber of housing container, simultaneously, broken aforementioned biological tissue; Prepare aforementioned tissue juice; Move dispensing apparatus through aforementioned suction and extract the filtration chamber that aforementioned tissue juice injects aforementioned housing container from the crushing chamber of aforementioned housing container, aforementioned filters mobile equipment moves the aforementioned filters of aforementioned filters anchor support, the filtration chamber that this filters is inserted aforementioned housing container; And filter aforementioned tissue juice, extraction is filtrated.
Fig. 1 is the entire infrastructure stereographic map of the apparatus for samples treatment of the kit, sample preparation of use an embodiment of the present invention.Fig. 2 is the stereographic map of the kit, sample preparation of an embodiment of the present invention, and Fig. 3 is the exploded view of the kit, sample preparation of an embodiment shown in Figure 2.Fig. 4～Figure 14 is the synoptic diagram of detailed structure of the kit, sample preparation of an embodiment shown in Figure 2.
Use the apparatus for samples treatment 100 of kit, sample preparation 1 of an embodiment of the present invention as shown in Figure 1, thereby be the device of the tissue juice extraction filtrating that obtains from this broken biological tissue at the broken after-filtration of biological tissue.As biological tissue, use be lymphoglandula.The lymphoglandula harvesting of pre-cutting is put into apparatus for samples treatment 100 shown in Figure 1 behind the specimen preparation container 10 of kit, sample preparation 1.At first, specify the structure of the kit, sample preparation 1 of an embodiment with reference to Fig. 2～Figure 14.
Kit, sample preparation 1 in this embodiment comprises following a few part as shown in Figures 2 and 3: specimen preparation container 10, embed specimen preparation container 10 lid 20, be used for the broken lymphoglandula that has cut off broken appliance 30, filter the tissue juice of obtaining from lymphoglandula filters 40, extract two packing 50 and 60 of filters 40 filtering filtratings out.Prestore broken liquid 70 and diluent 80 in the specimen preparation container 10 of kit, sample preparation 1.This fragmentation
Liquid 70 contains the non-ionics that destroys the membranin that contains lymphoglandula with diluent 80.The top surface portion 17 of specimen preparation container 10 (with reference to Fig. 5 and Fig. 6) is stained with filter paper 90 like Fig. 3 and shown in Figure 4, in case foreign material are sneaked into broken liquid 70 and diluent 80 from the outside.In addition, this kit, sample preparation 1 is each back depleted disposal package packing that uses.
Specimen preparation container 10 is like Fig. 3～shown in Figure 6, includes filters chamber 11, the broken appliance chamber 12 that is used to receive and keep broken appliance 30, the break process chamber 13 of depositing broken liquid 70, the filtration treatment chamber 14 of depositing diluent 80 that are used to receive and keep filters 40, takes in the packing head-room 15 of two packing 50 and 60.Each chamber 11～15 is as shown in Figure 6, becomes one with top surface portion 17 through reinforcing ribbed arch 16.Filters chamber 11, broken appliance chamber 12, break process chamber 13, filtration treatment chamber 14 and packing head-room 15 at one end have opening 11a, 12a, 13a, 14a and 15a respectively, and the other end has bottom 11b, 12b, 13b, 14b and 15b simultaneously.For another Fig. 3 and shown in Figure 6, side surface part 18, broken appliance chamber 12, break process chamber 13, filtration treatment chamber 14 and the packing head-room 15 of enclosing link with above-mentioned reinforcing rib arch 16 respectively around each chamber 11～15.In addition, between filters chamber 11 and the broken appliance chamber 12, between broken appliance chamber 12 and the break process chamber 13, between break process chamber 13 and the filtration treatment chamber 14 and filtration treatment chamber 14 also is equipped with packing head-room 15 and is reinforced ribbed arch 16.Top surface portion 17 also is equipped with the endways upright edge 19 that encloses around the top surface portion 17, in the outside of the filters chamber at this edge 19 11 1 sides and packing head-room 15 1 sides one projection 19a and 19b is arranged respectively also.
Filters chamber 11 is as shown in Figure 3 to be to establish for the filters 40 of taking in kit, sample preparation 1, and broken appliance chamber 12 is to establish for taking in broken appliance 30.And respectively there are a bearing surface 11c and 12c in filters chamber 11 and broken appliance chamber 12 like Fig. 5 and shown in Figure 6, and they see ovalize than top surface portion 17 certain depth that slightly caves in from the plane.Also jagged 11d in filters chamber 11 and 11e.Also there are jagged 12d and 12e in broken appliance chamber 12.And the breach 11e of breaking millstone tool chambers 11, filters chamber 12 1 sides leans on the breach 12e of filters chamber 11 1 sides to be connected with broken appliance chamber 12.
At this, in this embodiment, break process chamber 13 is to establish for using from the broken appliance 30 broken lymphoglandula of opening 13a insertion, and the lymphoglandula of pre-cutting is deposited in break process chamber 13 with broken liquid 70.The kit, sample preparation 1 of this embodiment is broken lymphoglandula in the broken liquid 70 of this break process chamber 13, obtains tissue juice.The bottom 1 of break process chamber 13
The interior bottom side of 3b such as Fig. 7 and shown in Figure 8 has semi-cylindrical hill 13c, with after the half-round recessed 31a (with reference to Fig. 9) that states on the interior crushing members 31 of broken appliance 30 flat-tops corresponding.This protruding 13c has and sees from the plane and to be criss-cross arch.The lymphoglandula that the recess 31a of this protruding 13c and interior crushing members 31 can clamp a certain size carries out fragmentation.The radius-of-curvature on protruding 13c surface is in fact the same with the surface curvature radius of recess 31a of crushing members 31 in the broken appliance 30.For another shown in Figure 8; The recess 31a at interior crushing members 31 flat-top places that is located at broken appliance 30 can space 13d occur between the protruding 13c of the recess 31a at interior crushing members 31 flat-top places and cruciform arcuation is with outside part with respect to the protruding 13c rotation of cruciform arcuation the time.
In this embodiment, filtration treatment chamber 14 is to establish for receiving and keeping broken lymphoglandula tissue juice that obtains and the tissue juice that filters harvesting.Kit, sample preparation 1 under this embodiment extracts the tissue juice that part obtains in break process chamber 13 in this filtration treatment chamber 14, and with the diluent that prestores 80 it is diluted, and filters the tissue juice of dilution then, extraction filtrating.Filtration treatment chamber 14 is like Fig. 3 and shown in Figure 6, and opening section is long-pending, and 14b one side is gradually little from opening part 14a one side direction bottom.
Packing the sequestration site 15c and the 15d that can take in packing 50 and 60 are respectively arranged in the packing head-room 15.
Lid 20 can embed specimen preparation container 10 as shown in Figures 2 and 3.Specifically, on the lid 20 the hook hole 20a corresponding with the filters chamber 11 1 lateral process 19a at specimen preparation container 10 edges 19 arranged, and the hook hole 20b corresponding with the projection 19b of packing head-room 15 1 sides.In view of the above, the hook hole 20a and the 20b interlock of projection 19a on the specimen preparation container 10 and 19b and lid 20 can will be covered 20 and embedded specimen preparation container 10.Lid 20 also has and the corresponding recess 20c of break process chamber 13 opening 113a, and this and the corresponding recess 20c of break process chamber 13 opening 13a can stop up break process chamber 13 opening 13a when lid 20 embeds specimen preparation container 10.After can preventing that like this tissue fluid extracting from finishing, will cover 20 embedding specimen preparation containers 10,13 opening 113a spill remaining tissue juice from the break process chamber.
Broken appliance 30 is like Fig. 9 and shown in Figure 10, is made up of with outer crushing members 32 interior crushing members 31, and wherein outer crushing members 32 is the tubular that can interior crushing members 31 be taken in it.Broken appliance 30 is to establish for the lymphoglandula that fragmentation is put with broken liquid 70.Interior crushing members 31 has a recess 31a corresponding with the protruding 13c (with reference to Fig. 7 and Fig. 8) of the break process chamber 13 of specimen preparation container 10 at its flat-top.As stated, there is the surperficial actual identical radius-of-curvature with the protruding 13c of break process chamber 13 on the surface of the recess 31a of this flat-top.Interior crushing members 31 with have the relative side of top one side of recess 31a that one fixer 31b is arranged, by after state the clamp clamps of apparatus for samples treatment 100.For another shown in Figure 9, a plurality of cutter 32a are arranged at the bottom of outer crushing members 32, from outwards outstanding ribbed arch 32b of surrounded surface and a plurality of slit 32c between a plurality of cutter 32a.The top of outer crushing members 32 such as Fig. 9 and shown in Figure 11, have the oval flange 32d corresponding with the oval bearing surface 12c (with reference to Fig. 5 and Fig. 6) of broken appliance chamber 12, can be with opening 32e, two breach 32fs and bending deformed several (this embodiment be 4) the hasp 32g of interior crushing members 31 incomes in it.Two breach 32f are corresponding with the breach 12d and the 12e (with reference to Fig. 5 and Fig. 6) of broken appliance chamber 12.
Filters 40 is established for filtering the tissue juice that obtains from the lymphoglandula of fragmentation, and purpose is the filtrating that sees through after 2 filter paper 41 and 42 (with reference to Figure 13) obtain filtration.Filters 40 is drum like Figure 12～shown in Figure 14, and the bottom has places 2 filter paper 41 with 42 filter paper placement part 40a with seal material placement part 40b up for safekeeping.In this embodiment, sealing material placement part 40b up for safekeeping does not have configuration to seal material up for safekeeping, but can dispose yet.40a is shown in figure 14 for the filter paper placement part, has to be seen as criss-cross filter paper fixer 40c from the plane.The filter paper fixer 40c of tissue juice through filter paper placement part 40a is pumped in the filters 40 with the space 43 of outside part.Filters 40 tops such as Figure 12 and shown in Figure 14; Have and the same structure in above-mentioned outer crushing members 32 tops, the oval flange 40d corresponding with the oval bearing surface 11c (with reference to Fig. 5 and Fig. 6) of filters chamber 11, opening 40e, two breach 40f and flexible several (this embodiment are 4) hasp 40g are arranged.Two breach 40f are corresponding with the breach 11d and the 11e (with reference to Fig. 5 and Fig. 6) of filters chamber 11.
What be placed on a packing sequestration site 15c is to establish for extracting the tissue juice that obtains into the lymphoglandula from fragmentation out; The packing 60 that is placed on a packing sequestration site 15d is to establish for extracting the filtrating that gets into filtration tissue juice out.As shown in Figure 3, this packing 50 have opening 50a and with opening 50a opposite end 50b.Packing 60 have too opening 60a and with opening 60a opposite end 60b.
Figure 15 and Figure 16 are respectively the stereographic map and the plats of apparatus for samples treatment main body shown in Figure 1.Figure 17～Figure 24 is the detailed structure view of apparatus for samples treatment each several part shown in Figure 1.Down
Face just uses the structure of apparatus for samples treatment 100 of the kit, sample preparation 1 of an embodiment to do a detailed description with reference to Fig. 1, Fig. 3, Fig. 6, Fig. 9, Figure 10, Figure 12, Figure 14～Figure 24.Apparatus for samples treatment 100 throw off cover 20 (with reference to Fig. 3) and filter paper 90 (with reference to Fig. 3) and load onto the kit, sample preparation 1 of the lymphoglandula of depositing pre-cutting after, the lymphoglandula deposited is implemented certain processing, can obtain filtrating.Apparatus for samples treatment 100 is as shown in Figure 1, is made up of indicating meter 110 and 3 main bodys 120 of showing certain information.Like Figure 15 and shown in Figure 16, main body 120 is moved dispensing apparatus 150, dismounting equipment 160 and unit 170 and is constituted by haulage equipment 130, sample processing apparatus 140, suction.Apparatus for samples treatment 100 is seen button 180, in addition off and is seen button 180 off by this, and the placing machine 131 of haulage equipment 130 just moves to the X1 direction, moves on to the outside from door 190.Kit, sample preparation 1 can install on the apparatus for samples treatment 100 in view of the above.
Haulage equipment 130 is made up of placing machine 131 and mobile equipment 132.This haulage equipment 130 has that the said sample of laying prepares the function of suit 1 and moves the function of kit, sample preparation 1 to directions X.
Placing machine 131 not only is used to lay kit, sample preparation 1, also is used to lay the sampling container 300 of harvesting filters 40 filtering filtratings.Placing machine 131 is like Figure 17 and shown in Figure 180, and it has the suit seating part 131a that lays kit, sample preparation 1, the sampling container seating part 131b that lays sampling container 300 and the filtrating of tissue juice in the kit, sample preparation 1 and sampling container 300 is controlled at the temperature regulator 131c (with reference to Figure 17) of certain temperature.
Be useful on insertion portion 131d, 131e, 131f, 131g and the 131h of filters chamber 11, broken appliance chamber 12, break process chamber 13, filtration treatment chamber 14 and the packing head-room 15 of laying specimen preparation container 10 respectively in the suit seating part 131a.Also have the ribbed arch insertion groove 131i corresponding in the suit seating part 131a with the reinforcing ribbed arch of specimen preparation container 10 16.
Sampling container seating part 131b has the container jack 131j (with reference to Figure 18) that places sampling container 300 and stops up the lid fixer 131k that sampling containers 300 opening 300a are provided with for the lid 301 that prevents to be installed on the sampling container 300.Temperature regulator 131c except that two fan 131l are arranged, also built-in no illustrated Peltier element, according to after state two fan 131l of wave control and the Peltier element that unit 170 (with reference to Figure 15 and Figure 16) sends.
Like Figure 15 and shown in Figure 16, mobile equipment 132 is to establish for move the placing machine 131 that disposes kit, sample preparation 1 to directions X.Mobile equipment 132 includes: phonomoter 132a, connect the pulley 132b of phonomoter 132a, the pulley 132c that establishes at a distance from certain intervals with pulley 132b, be contained in transmission belt 132d on pulley 132b and the pulley 132c, extend the moving-member 132f that the craspedodrome guiding 132e of configuration and the 132e that can lead along keeping straight on move and be connected with transmission belt 132d to directions X.So, phonomoter 132a drives the moving-member 132f that is connected on the transmission belt 132d and moves towards directions X through pulley 132b transmission belt 132d, and the placing machine 131 that is installed on the moving-member 132f can move along the guiding 132e that keeps straight on moving-member 132f.
Sample processing apparatus 140 can be used the lymphoglandula of depositing in broken appliance 30 (with reference to Fig. 9) and the broken kit, sample preparation 1 of filters 40 (with reference to Figure 12) in the kit, sample preparation 1, and filters the broken tissue juice that obtains.This sample processing apparatus 140 is made up of fixer 141 and mobile equipment 142.
Fixer 141 has fixing sample to prepare the function of the broken appliance 30 and the filters 40 of suit 1.Shown in figure 19, fixer 141 is useful on mounting portion 141a, the phonomoter 141b that is contained in 141a top, mounting portion, the placement part 141c that is used to place phonomoter 141b and mounting portion 141a, the metal sheet 141d that is contained in placement part 141c that broken appliance 30 and filters 40 are installed, the craspedodrome guiding 141e that is installed in metal sheet 141d, the moving-member 141f that can move along the guiding 141e that keeps straight on, is contained in hook 141g and spring 141h on the moving-member 141f.
Mounting portion 141a is shown in figure 20, comprise the pressing part 141i that locates to be pressed into from the opening 40e (with reference to Figure 14) of the outer crushing members 32 opening 32e of broken appliance 30 and filters 40 and with broken appliance 30 outside the jut 141j of breach 40f (with reference to Figure 14) interlock of breach 32f and filters 40 of crushing members 32.Pressing part 141i has groove 141k, and the hasp 32g of broken appliance 30 outer crushing members 32 and the hasp 40g (with reference to Figure 12) of filters 40 just in time are buckled in wherein.Like this; Outside the pressing part 141i of mounting portion 141a is pressed in the opening 40e of crushing members 32 opening 32e and filters 40; Hasp 32g and hasp 40g buckle among the groove 141k of pressing part 141i, thereby can be with broken appliance 30 and filters 40 141a fixation in the mounting portion.At this moment, the jut of mounting portion 141a
The breach 40f place that divides 141j to be stuck in the breach 32f and the filters 40 of outer crushing members 32,141a rotates in the mounting portion with filters 40 can to prevent outer crushing members 32.In addition, be provided with in the pressing part 141i with phonomoter 141b shown in Figure 19 and drive the no illustrated anchor clamps that rotate, can clamp fixer 31b (with reference to Fig. 9 and Figure 10) rotation of the interior crushing members 31 of broken appliance 30.Therefore, phonomoter 141b plays a role as the drive source of the interior crushing members 31 of rotation broken appliance 30.
Shown in figure 19, be contained in the hook 141l that is useful on an end of hanging spring 141h on the metal sheet 141d on the placement part 141c.The other end of spring 141h hangs on the hook 141g.So, moving-member 141f is moved as moving towards the Z direction to the Z direction as following mounting portion 141a.Resin system contact part 141m is arranged on the moving-member 141f, with after state the dismounting parts 161 of dismounting equipment 160 releasing pawl 161a contact.When broken appliance 30 and filters 40 were installed on the 141a of mounting portion, contact part 141m can contact with filters 40 flange 40d with outer crushing members 32 flange 32d (with reference to Fig. 9).
Mobile equipment 142 is to establish for moving fixer 141 to the Z direction like Figure 15 and shown in Figure 16.Mobile equipment 142 is made up of following several parts: phonomoter 142a; Be connected the pulley 142b on the phonomoter 142a; With the pulley 142c of pulley 142b at a distance from certain intervals; Be contained in the transmission belt 142d on pulley 142b and the pulley 142c; Can rotate with the rotation of pulley 142c; The snap head bolt 142e of vertically configuration (extending) along the Z direction; The craspedodrome guiding 142f of vertically configuration (extending) and the moving-member 142g that moves along the guiding 142f that keeps straight on snap head bolt 142e rotation to the Z direction.So; Phonomoter 142a drives transmission belt 142d through pulley 142b; Drive the pulley 142c rotation that transmission belt 142d connects; Snap head bolt 142e rotates with the rotation of pulley 142c, and mobile moving-member 142g moves along the Z direction of the guiding 142f that keeps straight on the rotation of snap head bolt 142e.
The function that suction moves dispensing apparatus 150 is: packing 50 and 60 (with reference to the Fig. 3) that kit, sample preparation 1 is installed can inhale and move packing tissue juice, and inhale and move the filtrating of packing from the tissue juice extraction.Suction moves dispensing apparatus 150 and moves mobile equipment 152 formations that suction moves dispensing apparatus 150 by the syringe of extracting tissue juice and filtrating out 151 and Z direction (vertically).
Syringe 151 is like Figure 21 and shown in Figure 22 comprising with the lower section: be equipped with packing 50 and 60 (with reference to Fig. 3) ozzle 151a, be contained in the resin cap on the ozzle 151a
The top 60b of 151b, the drive source phonomoter 151d that is used to inhale the pump 151c that moves extraction, pump 151c, the top 50b that surveys packing 50 and packing 60 whether contact liquid level detecting liquid level transmitter 151e, find out pump 151c and inhale the pressure acquisition sensor 151f that moves the pressure when extracting and the switchboard 151g of control detecting liquid level transmitter 151e and pressure acquisition sensor 151f.The opening 60a (with reference to Fig. 3) that the top 151h of ozzle 151a is pressed into opening 50a and the packing 60 of packing 50 installs packing 50 and 60.Resin cap 151b can with ozzle 151a relative to moving towards the Z direction, and this resin cap 151b has one edge 151i, when packing 50 and 60 is loaded onto, touches this packing head.
Mobile equipment 152 is made up of following several parts like Figure 15 and shown in Figure 16: phonomoter 152a, be connected pulley 152b on the phonomoter 152a, with pulley 152b at a distance from the pulley 152c of certain intervals, be contained in transmission belt 152d on pulley 152b and the pulley 152c, can rotate and towards the snap head bolt 152e of Z direction configuration, rotate and the moving-member 152g that moves along the guiding 152f that keeps straight on the rotation of pulley 152c to the craspedodrome guiding 152f of Z direction configuration and with snap head bolt 152e.So; Phonomoter 152a drives transmission belt 152d through pulley 152b; Drive the pulley 152c rotation that transmission belt 152d connects; Snap head bolt 152e rotates with the rotation of pulley 152c, and the moving-member 152g mobile with the rotation of snap head bolt 152e moves along the Z direction that the guiding 152f that keeps straight on disposes.
Dismounting equipment 160 has dismounting and is fixed on the broken appliance 30 (with reference to Fig. 9) and the filters 40 (with reference to Figure 12) of fixer 141 and is contained in the function that suction moves the packing 50 and 60 (with reference to Fig. 3) on the dispensing apparatus 150.Dismounting equipment 160 also can prevent tissue juice that the broken appliance 30 after use the drips each several part attached to specimen preparation container 10 and apparatus for samples treatment 100, and a packing after can suppressing to use 50 and 60 tissue juice and the each several parts of filtrating attached to specimen preparation container 10 and apparatus for samples treatment 100 that drip down.Dismounting equipment 160 has the mobile equipment 162 that moves dismounting parts 161 from the dismounting parts 161 of fixer 141 dismounting broken appliances 30 and filters 40 with to directions X like Figure 15, shown in Figure 16.
Dismounting parts 161 are like Figure 23 and shown in Figure 24, comprise the releasing pawl 161a of contact on fixer 141 contact part 141m, with inhale the packing head releasing hole 161b that the edge 151i that moves dispensing apparatus 150 lid 151b contacts and connect tissue juice and the resin of filtrating connects and coils 161c.
Mobile equipment 162 is made up of following several parts like Figure 15, Figure 16 and shown in Figure 23: phonomoter 162a, be connected pulley 162b on the phonomoter 162a, with pulley 162b at a distance from the pulley 162c of certain intervals, be contained in the craspedodrome guiding 162e of transmission belt 162d on pulley 162b and the pulley 162c, directions X configuration and the moving-member 162f that can move along the guiding 162e that keeps straight on.So, phonomoter 162a drives transmission belt 162d through pulley 162b, drives the moving-member 162f that is connected on the transmission belt 162d and moves to directions X.So the dismounting parts 161 that are contained on the moving-member 162f can move to directions X.
Unit 170 is like Figure 15 and shown in Figure 16, for control apparatus for samples treatment 100 each several parts are established.Specifically, unit 170 and each mobile equipment 132,142,152 are connected with 162 phonomoter 132a, 142a, 152a and 162a, can send the wave that each phonomoter 132a of control, 142a, 152a and 162a drive.Through moving of wave control placing machine 131, fixer 141, syringe 151 and dismounting parts 161.Unit 170 also is connected with Peltier element (not having diagram) with the fan 131l of the temperature controller 131c of placing machine 131; Can send the rotation of this fan of control 131l and the wave of Peltier element, the filtrating in tissue juice in the kit, sample preparation 1 and the sampling container 300 is controlled at certain temperature with this.
Figure 25～Figure 50 is the synoptic diagram of the apparatus for samples treatment extraction filtrating process of the kit, sample preparation of use an embodiment of the present invention.Describe with regard to the process of apparatus for samples treatment 100 extraction filtratings with reference to Fig. 3, Figure 12, Figure 14～Figure 16, Figure 19～Figure 50 below.
At first, prepare kit, sample preparation 1: pluck from specimen preparation container 10 (with reference to Fig. 3) and cover 20 (with reference to Fig. 3) and film 90 (with reference to Fig. 3), and the lymphoglandula 200 that will cut off is in advance put into break process chamber 13.Again by apparatus for samples treatment 100 see button 180 off, ready kit, sample preparation 1 is put on the placing machine of seeing off 131.Shown in figure 25 then, the phonomoter 132a (with reference to Figure 15 and Figure 16) that opens mobile equipment 132 moves placing machine 131 to the X1 direction, makes fixer 141 mounting portion 141a and broken appliance chamber 12 horizontal directions (directions X) aligned in position of depositing broken appliance 30.In this state, the phonomoter 142a (with reference to Figure 15 and Figure 16) that starts mobile equipment 142 moves fixer 141 to Z1 direction (below).At this moment, shown in figure 20, the breach 32f of outer crushing members 32 and the jut 141j of mounting portion 141a sting
Close, hasp 32g snaps in the groove 141k of mounting portion 141a simultaneously, thereby broken appliance 30 is assembled into mounting portion 141a.After this, move fixer 141, become state shown in Figure 26 to Z2 direction (top).
Shown in figure 27, move placing machine 131 to the X1 direction, make mounting portion 141a that broken appliance 30 is housed and break process chamber 13 horizontal directions (directions X) aligned in position that has lymphoglandula 200 and broken liquid 70.Through moving fixer 141 broken lymphoglandula 200 in break process chamber 13 to Z1 direction (below).Specifically, at first shown in figure 28, through to Z1 direction (below) and Z2 direction (top) repeated moving broken appliance 30, lymphoglandula 200 is crushed to institute's sizing.Shown in figure 29 then; Crushing members 31 in the phonomoter 141b (with reference to Figure 19) that starts fixer 141 rotates in a certain size lymphoglandula 200 is the broken liquid 70 of floating state; Make lymphoglandula 200 shown in figure 30 in broken liquid 70 further refinement, homogenizing, generate tissue juice.
Next shown in figure 31, through moving the mounting portion 141a that broken appliance 30 is housed, broken appliance 30 is extracted out from tissue juice to Z2 direction (top).The phonomoter 162a (with reference to Figure 15, Figure 16 and Fig. 2 3) that restarts mobile equipment 162 will dismantle parts 161 move to lower position from the broken appliance 30 of extraction to the X2 direction.At this moment, the tissue juice even the broken appliance of extracting out from tissue juice 30 drips, the meeting dish 161c (with reference to Figure 23 and Figure 24) and also can catch of dismounting parts 161.
Then, shown in figure 32, move placing machine 131 to the X2 direction, move dismounting parts 161 to the X1 direction simultaneously, the mounting portion 141a that broken appliance 30 is housed is alignd with broken appliance chamber 12 levels (directions X).Shown in figure 33 afterwards, move fixer 141 to Z1 direction (below), broken appliance 30 is inserted broken appliance chamber 12.Specifically, shown in figure 33, when Z1 direction (below) moves fixer 141, move dismounting parts 161 to the X2 direction, make the contact part 141m of the releasing pawl 161a of dismounting parts 161 over against fixer 141.Under this state; Move fixer 141 to Z2 direction (top); Make the bottom of the flange 32d extruding contact part 141m of broken appliance 30; Thereby one is on the flange 32d of power crushing members 32 outside contact part 141m is added to of Z1 direction (below) (extracing the direction of broken appliance 30 from mounting portion 141a), and 141a breaks away from from the mounting portion to make outer crushing members 32 (broken appliance 30).
Then, shown in figure 34, move placing machine 131 to the X1 direction, the ozzle 151a that makes syringe 151 aligns with the packing head harvesting place 15c level (directions X) of the packing head-room 15 of depositing packing 50.Under this state, the phonomoter 152a (with reference to Figure 15 and Figure 16) that opens mobile equipment 152 moves syringe 151 to Z1 direction (below).At this moment, shown in figure 22, the opening 50a of packing 50 is pressed into the top 151h of ozzle 151a, thereby packs ozzle 151a into.Then, move syringe 151 to Z2 direction (top) and become state shown in Figure 35.
Shown in figure 36 again, move placing machine 131 to the X1 direction, the ozzle 151a that loads onto packing 50 is alignd with tissue juice income break process chamber 13 levels (directions X) wherein.Shown in figure 37 then, move syringe 151 to Z1 direction (below), extract the tissue juice in the break process chamber 13.Specifically, find out after top 50b that transmitter 151e (with reference to Figure 21) detects packing 50 contacts the liquid level of tissue juice when liquid level, pump 151c (with reference to Figure 21) leans on the power of phonomoter 151d (with reference to Figure 21) to begin extracting interstitial fluid.Then, move the ozzle 151a that packing 50 has been installed, packing 50 is withdrawn from from tissue juice to Z2 direction (top).Move the below of dismounting parts 161 a to packing of withdrawing from 50 to the X2 direction, after the X1 direction moves placing machine 131, move dismounting parts 161, the ozzle 151a that packing 50 is housed is alignd with filtration treatment chamber 14 levels (directions X) to the X1 direction.
Then, shown in figure 38, move syringe 151 to Z1 direction (below), will be in the break process chamber 13 tissue juice that suck inject filtration treatment chamber 14 and stir.Specifically, find out after top 50b that transmitter 151e (with reference to Figure 21) detects packing 50 touches the liquid level of diluent when liquid level, pump 151c (with reference to Figure 21) leans on the power of phonomoter 151d (with reference to Figure 21) to begin to release tissue juice.At this moment, with pump 151c in the diluent of sneaking into tissue juice, extract out repeatedly, suction operation, stir tissue juice.
Next through move the ozzle 151a that packing 50 is housed to Z2 direction (top); With the tissue juice after the packing 50 proposition dilution; Move dismounting parts 161 to packing 50 below that propose to the X2 direction; Move placing machine 131 to the X1 direction again, move dismounting parts 161 to the X1 direction simultaneously, the ozzle 151a that packing 50 is housed is alignd with the packing head harvesting place 15c level (directions X) of packing head-room 15.Shown in figure 39 subsequently, move syringe 151 to Z1 direction (below), packing head harvesting place 15c is taken in packing 50.Specifically, move syringe 151, move dismounting parts 161 to the X2 direction simultaneously, make the packing head of dismounting parts 161 remove the edge 151i top that hole 161b (with reference to Figure 24) just in time is positioned at the lid 151b that moves past the syringe 151 that comes to Z1 direction (below).Under this state; Moving syringe 151 to Z2 direction (top) again makes packing 50 extruding cover the 151i bottom, edge of 151b; Thereby power to Z1 direction (below) (extracing the direction of packing 50 from ozzle 151a) is added to from lid 151b in the packing 50, makes packing 50 break away from ozzle 151a.
Below shown in figure 40, after the X1 direction moves placing machine 131, move fixer 141 to Z1 direction (below), fixer 141 mounting portion 141a are alignd with the filters chamber 11 levels (directions X) of depositing filters 40.At this moment, the breach 40f (with reference to Figure 14) of filters 40 and the jut 141j interlock of mounting portion 141a, hasp 40g (Figure 12 and Figure 14) snaps in mounting portion 141a groove 141k (with reference to Figure 20) simultaneously, and filters 40 is installed into mounting portion 141a.Then, move fixer 141, become state shown in Figure 41 to Z2 direction (top).
Shown in figure 42, move placing machine 131 to the X1 direction, align in the filtration treatment chamber 14 levels (directions X) of the tissue juice after making mounting portion 141a that filters 40 is housed and taking in dilution.Move the tissue juice of fixer 141 after the dilution of filtration treatment chamber 14 inner filtrations to Z1 direction (below) then.Specifically, at first shown in figure 43, move fixer 141 to Z1 direction (below), make filters 40 insert filtration treatment chamber 14.At this moment, because of filtration treatment chamber 14 to be shaped as opening section long-pending gradually little to bottom 14b by opening 14a, so the tissue juice after can preventing to dilute spills from the space between filters 40 sides and the filtration treatment chamber 14.Shown in figure 44, the diluted tissue juice between filters 40 and the filtration treatment chamber 14 sees through 2 filter paper 41 and 42 and is extracted in the filters 40 of tubular.
Shown in figure 45, move dismounting parts 161 to the X2 direction, make the releasing pawl 161a that dismantles parts 161 just in time be positioned at contact part 141m (with reference to Figure 19) top of fixer 141.Under this state; Move fixer 141 to Z2 direction (top); The flange 40d (with reference to Figure 12) of filters 40 will push contact part 141m bottom; Thereby make one power add flange 40d, make filters 40 disengaging mounting portion 141a, stay filtration treatment chamber 14 to filters 40 from contact part 141m to Z1 direction (below) (direction of 141a dismounting filters 40) from the mounting portion.
Next move placing machine 131 to the X2 direction, move to Z1 direction (below) simultaneously
Moving syringe 151 makes syringe 151 ozzle 151a align with packing storeroom 15d level (directions X) of the packing head-room 15 of taking in packing 60.At this moment, packing 60 an opening 60a (with reference to Fig. 3) are pressed into the top 151h (with reference to Figure 22) of 151a, install on the ozzle 151a.After this, move syringe 151, move placing machine 131 to the X2 direction simultaneously, the ozzle 151a that loads onto packing 60 is alignd with the filtration treatment chamber 14 levels (directions X) of taking in filtrating, form state shown in Figure 46 to Z2 direction (top).
After Z1 direction (below) moves syringe 151, shown in figure 47, extract the filtrating in the filtration treatment chamber 14.Then, move the ozzle 151a that packing 60 is housed, propose packing 60 from filtrating to Z2 direction (top).Move the below of dismounting parts 161 a to packing of withdrawing from 60 again to the X2 direction; After this shown in figure 48; After the X1 direction moves placing machine 131 and makes ozzle 151a that packing 60 is housed and sampling container 300 levels (directions X) of the sampling container seating part 131b that is placed on placing machine 131 are alignd, move dismounting parts 161 to the X1 direction.
Then, shown in figure 49, move syringe 151 to Z1 direction (below), simultaneously the filtrating in the filtration treatment chamber 14 is injected in the sampling container 300, the result can obtain the filtrating that obtains from lymphoglandula 200 in sampling container 300.
Move the ozzle 151a that packing 60 is housed, the below of moving dismounting parts 161 to the branch head 60 that proposes simultaneously to the X2 direction to Z2 direction (top).Move placing machine 131 and move dismounting parts 161 to the X2 direction again, the ozzle 151a that packing 60 is housed is alignd with the packing head harvesting place 15d level (directions X) of packing head-room 15 to the X1 direction.
Shown in figure 50, move syringe 151 to Z1 direction (below), packing head harvesting part 15c is put in packing 50.And then, packing 60 is separated from ozzle 151a with the step same with removing packing 50.
As stated, the filtrating extraction process of apparatus for samples treatment 100 is accomplished.
In this embodiment; As stated; The bottom 13b inboard of break process chamber 13 is corresponding with the half-round recessed 31a of the interior crushing members 31 of broken appliance 30; Be semi-cylindrical hill 13c design, the broken appliance 30 of coming in from opening 13a like this is easy to arrive the inboard protruding 13c of (touching) bottom 13b, can prevent the broken appliance 30 that inserts from opening 13a with this and touch the part (wasted space) less than the protruding 13c of break process chamber 13 bottom 13b.Thereby avoid occurring lymphoglandula 200 get into broken appliances 30 touch less than the place, make the broken appliance 30 that inserts from opening part 13a be able to practical broken lymphoglandula 200.
In this embodiment; In fact the surface of the surface of the protruding 13c of break process chamber 13 bottom 13b and interior crushing members 31 recess 31a has the same curvature radius; Therefore; Can clamp the primary lymphedema knot 200 that has been crushed to a certain degree between the protruding 13c of interior crushing members 31 recess 31a and break process chamber 13 bottom 13b, thereby make lymphoglandula 200 trickleer.
In this embodiment; 13b inboard, 13 bottom, break process chamber is provided with the protruding 13c of cruciform ribbed arch shape; As the recess 31a of the flat-top of interior crushing members 31 during with respect to the protruding 13c rotation of cruciform ribbed arch shape; Form gap 13d between the elswhere beyond the recess 31a of interior crushing members 31 flat-tops and the protruding 13c of break process chamber 13; Make lymphoglandula 200 be able to get among the gap 13d that forms between interior crushing members 31 recess 31a and the elswhere beyond the 13 protruding 13c of break process chamber, if this moment just can be along with the lymphoglandula 200 that gets into gap 13d is rolled in the rotation of interior crushing members 31 with respect to the interior crushing members 31 of protruding 13c rotation.Because can clamp the lymphoglandula of rolling 200 between the protruding 13c of interior crushing members 31 recess 31a and break process chamber 13, therefore can broken more effectively lymphoglandula 200.
In this embodiment; Because of filtration treatment chamber 14 to be shaped as opening section long-pending gradually little to bottom 14b by opening 14a; So can prevent from opening 14a when bottom 14b inserts filters 40; The periphery of filters 40 contacts with filtration treatment chamber 14 inwalls when reaching certain insertion amount, and tissue juice overflows from filters 40 sides.2 filter paper 41 and 42 through being contained on the filters 40 filter paper placement part 40a can extract filtrating.As stated, be easy to be extracted into the tissue juice between filters 40 and the filtration treatment chamber 14 bottom 14b, thereby also be easy to extraction filtrating from the tissue juice that broken lymphoglandula 200 obtains through the filter paper 41 and 42 of filters 40.
In this embodiment; Broken appliance 30 is made up of with outer crushing members 32 interior crushing members 31; Wherein interior crushing members 31 has recess 31a corresponding with the break process chamber 13 protruding 13c of specimen preparation container 10, and outer crushing members 32 is made up of the tubular article of crushing members 31 in can taking in, and simultaneously interior crushing members 31 can rotate for outer crushing members 32 relatively; Therefore, can the broken like a cork lymphoglandula 200 that gets between interior crushing members 31 and the outer crushing members 32.Like this can be between interior crushing members 31 recess 31a and break process chamber 13 protruding 13c and interior crushing members 31 and outside broken lymphoglandula 200 aspect two between the crushing members 32, the fragmentation work that makes lymphoglandula 200 is conscientiously and rapidly.
In this embodiment; Form a plurality of slit 32c through crushing members outside 32, make between interior crushing members 31 recess 31a and break process chamber 13 protruding 13c and interior crushing members 31 and outer crushing members 32 between broken lymphoglandula 200 through slit 32c from interior crushing members 31 and outside the outside of crushing members 32 outside the flow direction between the crushing members 32.With this can prevent lymphoglandula 200 be trapped between crushing members 31 and the outer crushing members 32, lymphoglandula 200 is circulated between the outside the outer crushing members 32 of tubular in, so as better interior crushing members 31 and outside crushing members 32 broken lymphoglandula 200.
In this embodiment; As stated; The fixer 141 of sample processing apparatus 140 fixedly top has opening 40e, bottom that the cylinder-shaped filter tool 40 of filter paper 41 and 42 is arranged; And move this filters 40 with mobile equipment 142, and insert the filtration treatment chamber 14 that harvesting has the specimen preparation container 10 of the tissue juice that broken lymphoglandula obtains, can there be the filters 40 of opening 40e to filter the tissue juice of harvesting with top in filtration treatment chamber 14.Because of it can be extracted into filters 40 inner structures for filtrating; So the opening 40e that can pass through filters 40 tops is with in the packing 60 insertion filters 40; With this packing 60 extraction filtrating, thereby obtain through filtering the filtrating of broken lymphoglandula gained tissue juice extraction.
In this embodiment; As stated; Suction moves dispensing apparatus 150 and is equipped with to be used to inhale and moves the packing 60 of extracting filtrating, and available above-mentioned suction moves dispensing apparatus 150 (packing 60) and extracts the filtrating of extraction through the opening 40e of filters 40, and the filtrating that will the extract sampling container 300 of packing into.So, can be at an easy rate move dispensing apparatus 150 (packing 60) and take filtrating with inhaling.
In this embodiment; As stated, inhale and to move dispensing apparatus 150 packing 50 also can be installed, be used to extract tissue juice in 13 preparations of the break process chamber of specimen preparation container 10; Simultaneously the tissue juice that extracts is injected the filtration treatment chamber 14 of specimen preparation container 10; There is no need to establish in addition one will 13 preparations in the break process chamber tissue juice transport to the equipment of filtration treatment chamber 14, thereby cut down components number, realized the miniaturized of device.
In this embodiment; As stated; There is the fixer 141 of sample processing apparatus 140 can fix the broken appliance 30 of broken lymphoglandula; This fixer 141 both can be when broken lymphoglandula have prepared tissue juice fixing broken appliance 30, again can be when filtering tissue juice Fixed-Filter tool 40, thereby make the preparation of tissue juice and filter and can in same device, accomplish.In same device, accomplish the preparation of tissue juice and filter and can also establish not only fixedly broken appliance 30 more in addition but also can broken appliance 30 have been inserted the equipment of break process chambers 13, thereby also can reach the effect of reduction components number, implement device miniaturized.
In this embodiment; As stated; Also have mobile equipment 142 to be used to move up and down fixer 141, when fixer 141 be configured in be higher than specimen preparation container 10 LZT, can move down fixer 141 through mobile equipment 142; Broken easily lymphoglandula prepares tissue juice, and filters tissue juice.
In this embodiment; As stated; The fixer 141 of sample processing apparatus 140 is provided with the pressing part 141i that is pressed into filters 40 and broken appliance 30; As long as filters 40 and broken appliance 30 are pressed into this pressing part 141i, can be like a cork through fixer 141 Fixed-Filter tools 40 and broken appliance 30.At this moment; If do not use filters 40 and broken appliance 30 to be placed in the specimen preparation container 10, then can move down do not use filters 40 and the broken appliance 30 that fixer 141 will be placed in the specimen preparation container 10 like a cork and be pressed into the pressing part 141i that is fixed on fixer 141 with mobile equipment 142
In this embodiment; As stated; Dismounting equipment 160 be used for from fixer 141 dismounting be fixed on the fixer 141 of sample processing apparatus 140 filters 40 and broken appliance 30 at least one of them, therefore, when the tissue juice preparation section gets into tissue juice and filters operation; Can directly dismantle broken appliances 30 from fixer 141 with dismounting equipment; Load onto filters 40, and when filtering operation entering tissue juice preparation section from tissue juice, available again dismounting equipment 160 is dismantled filters 40, is loaded onto broken appliance 30 from fixer 141.
In this embodiment, as stated, when mobile equipment 162 moves horizontally dismounting during parts 161 to removed position, mobile equipment 142 fixer 141 that can move up, one of them breaks away from from fixer 141 at least with broken appliance 30 to make filters 40.Although the fixer 141 of Fixed-Filter tool 40 and broken appliance 30 can only move up and down, through move up and down fixer 141 with move horizontally dismounting parts 161 just can be at an easy rate from fixer 141 dismounting filters 40 and broken appliance 30 at least one of them.
In this embodiment; As stated; Owing to be useful on the placing machine 131 of laying specimen preparation container 10 and the mobile equipment 132 that moves horizontally this placing machine 131; Although fixer 141 can only move up and down,, can make the fixer 141 of Fixed-Filter tool 40 and the filtration treatment chamber 14 of specimen preparation container 10 be in same level attitude when filtering tissue juice as long as move horizontally the placing machine 131 that specimen preparation container 10 is housed with mobile equipment 132; Preparation can make during tissue juice fixedly, and the fixer 141 of broken appliance 30 and the break process chamber 13 of specimen preparation container 10 are in same level attitude.Like this, although fixer 141 can only move up and down, the filtration treatment chamber 14 that can filters 40 be inserted specimen preparation containers 10 when filtering tissue juice, the break process chamber 13 that can broken appliance 30 be inserted specimen preparation containers 10 during preparation tissue juice.
In addition, this time disclosed embodiment can think to be illustration in all respects, never is defined in this.Scope of the present invention is not limit by the statement of foregoing invention content only by appended claim book defined, and comprise with claim scope equivalence and scope in all mutation.
Such as, in the above-mentioned embodiment illustration broken liquid chamber bottom inside the design that is seen as semicircular convexity from the front is arranged, but the present invention is not limited only to this, and semicircle convex design with shape also can be arranged.For example also can be designed to the quadrate convexity.At this moment, corresponding with the quadrate convexity, the top of the interior crushing members of broken appliance also must be designed to the quadrate recess.
In the above-described embodiment, when interior crushing members rotated, for the part beyond interior crushing members recess and broken liquid chamber convexity forms the gap, convexity was designed to the plane and is seen as cruciform ribbed arch shape.But the present invention is not limited only to this, also can not establish the gap between interior crushing members recess and the broken liquid chamber convexity.
In the above-described embodiment, the broken appliance of broken biological tissue is made up of interior crushing members and outer crushing members.But the present invention is not limited only to this, the broken biological tissue of an also available crusher members.
In the above-described embodiment, broken appliance inserts the break process chamber that is placed with lymphoglandula and broken liquid from the top, move up and down this broken appliance lymphoglandula is crushed to a certain size, rotates the interior crushing members of broken appliance again, with the further refinement of lymphoglandula.But the present invention is not limited only to this, also can broken appliance be inserted from the top and be placed with the break process chamber of lymphoglandula and broken liquid, Yi Bian move up and down this broken appliance, comes broken lymphoglandula Yi Bian rotate the interior crushing members of broken appliance.
In the above-described embodiment, only be provided with a sampling container, extract after the filtering filtrating in filtration treatment chamber, inject a sampling container with the packing head.But the present invention is not limited only to this; Two sampling containers also can be set; And the diluent that will be placed on the filtration treatment chamber in advance injects one of them sampling container with the packing head; Filtrating with the packing head will inject in another sampling container is released, and is put into previous sampling container, with the preparation dilute sample.
In the above-described embodiment, introduced the suitable examination for preparing and filter tissue juice of the present invention
The appearance treatment unit, but the present invention is not limited only to this, and the present invention also is applicable to the apparatus for samples treatment that can only filter tissue juice.
In the above-described embodiment, used broken appliance and the filters can both the fixed fixer, but the present invention is not limited only to this, also fixedly the fixer of the fixer of broken appliance and Fixed-Filter tool be respectively established one.
In the above-described embodiment; Extracting filtrating and transferring tissue juice from the break process chamber to the filtration treatment chamber is to move dispensing apparatus with a suction; But the present invention is not limited only to this, also can extract filtrating and transfers tissue juice from the break process chamber to the filtration treatment chamber and respectively establish a suction and move dispensing apparatus.
In the above-described embodiment, broken appliance inserts the break process chamber that is placed with lymphoglandula and broken liquid from the top, move up and down this broken appliance lymphoglandula is crushed to a certain size, rotates the interior crushing members of broken appliance again, with the further refinement of lymphoglandula.But the present invention is not limited only to this, also can broken appliance be inserted from the top and be placed with the break process chamber of lymphoglandula and broken liquid, Yi Bian move up and down this broken appliance, comes broken lymphoglandula Yi Bian rotate the interior crushing members of broken appliance.
In the above-described embodiment, only be provided with a sampling container, extract after the filtering filtrating in filtration treatment chamber, inject in the sampling container with the packing head.But the present invention is not limited only to this; Two sampling containers also can be set, and the diluent that will be placed on the filtration treatment chamber is in advance released with the filtrating that the packing head will inject in another sampling container with one of them sampling container of packing head injection; Be put into the aforementioned sample container, with the preparation dilute sample.
Scope of the present invention is not limit by above statement on any degree only by appended claim book defined.

Claims (6)

1. specimen preparation container comprises:
The first harvesting chamber, the one of which end is provided with first opening, and the other end is provided with the first protruding bottom of bottom inside, and the broken appliance that inserts from aforementioned first opening therein carries out biological tissue's fragmentation;
The convexity of first bottom of the wherein said first harvesting chamber can with the vertical recess clamping of said broken appliance and broken biological tissue.
2. specimen preparation container as claimed in claim 1, the convexity of first bottom of the wherein said first harvesting chamber has identical radius-of-curvature with the vertical recess of said broken appliance.
3. specimen preparation container as claimed in claim 1; The convexity of first bottom of the wherein said first harvesting chamber is the ribbed arch shape in said first bottom; When the vertical recess of said broken appliance rotates with respect to said ribbed arch shape is protruding, form the gap between the part beyond the ribbed arch shape convexity of first bottom of vertical recess of said broken appliance and the said first harvesting chamber.
4. specimen preparation container as claimed in claim 1 wherein also has the second harvesting chamber, is used to receive and keep the tissue juice that broken biological tissue obtains in the said first harvesting chamber, filters the tissue juice of aforementioned harvesting simultaneously.
5. specimen preparation container as claimed in claim 4, the wherein said second harvesting chamber one end is provided with second opening, the other end is provided with second bottom, its opening part sectional area from said second opening towards the said second bottom convergent.
6. specimen preparation container as claimed in claim 4, wherein said first harvesting chamber and the said second harvesting chamber are one.
CN2006101410600A 2005-09-29 2006-09-28 Sample preparation kit, sample preparation container, and sample processing device Active CN1940055B (en)

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