JP4660487B2 - Cosmetic composition for increasing skin whitening effect - Google Patents

Description

  The present invention relates to a cosmetic composition capable of enhancing the whitening effect by suppressing the excessive production of melanin and promoting the discharge of melanin produced in the skin, and more particularly, red ginseng extract The present invention relates to a cosmetic composition containing white birch extract and papain enzyme and capable of increasing the whitening effect of licorice extract which is a skin whitening component.

  When the skin is exposed to ultraviolet rays, melanin, which determines the color of human skin, is synthesized and released, causing hyperpigmentation such as stains, freckles, and spots, resulting in poor cosmetic results. Come to bring.

  The process of synthesizing melanin has a mechanism in which intracellular tyrosine is used as a substrate and tyrosinase enzyme acts to form dopaquinone, and melanin is generated from dopaquinone. Therefore, in order to prevent the skin from becoming dark, methods for reducing melanin synthesis itself by inhibiting any specific process in the melanin production process are generally studied. Whitening agents developed in this way include kojic acid, arbutin, licorice extract, vitamin A and vitamin C.

  However, melanin not only plays a role in determining skin color, but also forms a hat-like structure around the nucleus, protecting genes from ultraviolet rays, removing free radicals, and protecting intracellular proteins. I am in charge of an important role. As a result, there is a problem that excessive suppression of melanin production itself may not perform the necessary minimum role in the skin.

  Therefore, it appears that an access method is needed that brightens the skin tone by selectively and quickly removing only the excessively produced melanin.

  Accordingly, the present inventors have studied to promote skin whitening in three aspects in conjunction with the skin structure in order to solve the above-described problems.

  As a result, firstly, papain enzyme is used to enhance the detachment of the outermost cells in the stratum corneum of the skin, and secondly, red ginseng extract is used to promote cell growth. And, thirdly, by using white cocoon extract to soften rough skin, the overall skin turnover mechanism can be maximized, and as a result, it can enhance the whitening effect on the skin As a result, the present invention has been completed.

  Accordingly, an object of the present invention is to provide a method capable of maximizing the whitening effect of a skin whitening component. Thus, the present invention provides a cosmetic composition containing a red ginseng extract, white birch extract and papain enzyme as a composition capable of maximizing the whitening effect of the skin whitening component.

  Another object of the present invention is to provide a whitening cosmetic composition containing a red ginseng extract, white birch extract and papain enzyme, and containing a licorice extract as a whitening component.

  The present invention provides a whitening composition containing a licorice extract, red ginseng extract, white birch extract and papain enzyme.

  The composition according to the present invention relates to a cosmetic composition capable of enhancing the whitening effect as a result by suppressing the excessive production of melanin and promoting the discharge of melanin produced in the skin. Through the cell detachment promotion, cell generation promotion and skin softening, the turnover cycle of the whole skin can be accelerated.

  The present inventor shows that the licorice extract, red ginseng extract, white birch extract and papain enzyme increase to an extent that the whitening effect cannot be expected when used simultaneously as compared to the case where each is used alone. Discovered for the first time. That is, the said component can maximize the whitening effect by using a mixture with each other. Accordingly, the present inventors named the whitening composition containing the licorice extract, red ginseng extract, white birch extract and papain enzyme as “white energy complex”.

  First, in this invention, the said licorice extract acts as a main component of the whitening activity for melanin production suppression. The content of the licorice extract is preferably 0.001 to 2.0% by weight with respect to the total weight of the composition. This is because the whitening effect is minute at a concentration of less than 0.001% by weight, and skin irritation is induced at a concentration of more than 2.0% by weight.

  The papain enzyme mainly functions to promote cell detachment, but is preferably 0.01 to 0.1% by weight based on the total weight of the composition. This is because the effect is insignificant at a concentration of less than 0.01% by weight and induces skin irritation at a concentration exceeding 0.1% by weight.

  Red ginseng extract is mainly responsible for promoting cell growth. Preferably, 6-year-old red ginseng is suitable in terms of price and efficacy. The amount used is preferably 0.01 to 5.0% by weight based on the total weight of the composition. If the concentration is less than 0.01% by weight, it is difficult to expect the effect, and if the concentration exceeds 5.0% by weight, a cost is incurred.

  Birch extract contributes to skin softening. The amount used is preferably 0.01 to 5.0% by weight based on the total weight of the composition. At concentrations below 0.01% by weight, it is difficult to expect an effect, and at concentrations above 5.0% by weight, irritation can be induced.

  In addition to each of the above-described effects, the effects of the components have a greater utility of causing a synergistic effect by being mixed with each other.

  In addition to licorice extract, kojic acid, arbutin, vitamin A, vitamin C and the like can be used as the main component of the whitening activity.

Hereinafter, the present invention will be described in detail.
Most whitening agents developed so far have been able to reduce the amount of melanin by inhibiting the activity of tyrosinase, the basic and most important enzyme essential for melanin biosynthesis. It was. The amount of use of the whitening agent thus developed is limited by the side effects that induce skin irritation, and it is actually difficult to use an amount sufficient to show a satisfactory whitening effect. From this, the composition of the present invention is superior to the conventional whitening agent by using a system that enhances the activity of skin turnover in order to enhance the whitening effect while using the main whitening ingredients showing the whitening effect as they are. The whitening effect can be shown.

  The skin is composed of a large, shallow epidermis and a deeper dermis (dermis). The lower part of the dermis is called a subcutaneous tissue. There is a connective tissue layer (subcutaneous tissue). The epidermis is the outermost layer of the skin composed of epithelial tissue, and is composed of all five layers. Specifically, it consists of a basal layer, a spinous layer, a granular layer, a transparent layer, a clear layer, and a horny layer from the bottom, followed by new cells from the bottom. The dead cells keratinized while pushing up the cells gradually become detached. A cycle in which new cells are generated at the bottom of the epidermis of the skin and the old cells are pushed out of the skin and separated from the body is called a turn-over cycle. By facilitating a turn-over cycle, melanin produced in the epidermis of the skin can be quickly discharged out of the body.

  Hereinafter, preferred embodiments of the present invention will be described in detail based on examples and comparative examples. However, the present invention is not limited to these examples.

<Examples 1 and 2> Production of whitening composition (white energy complexes 1 and 2)
The licorice extract contains 35% or more of grubridin from Maruzen Pharmaceuticals Co. Ltd (Japan), and used as a product name Polysoluble soluble licorice ext PT (40).

  The red ginseng extract used was obtained by using a 6-year-old red ginseng extract from Koze Co., Ltd. using an enzymatic decomposition method.

[Enzymatic degradation method]
10 g of red ginseng extract was dissolved in 100 ml of citrate buffer solution (pH 5.5), 1 g of naringinase enzyme separated from Penicillium was added thereto, and the mixture was allowed to react for 48 hours while stirring on a 40 ° C. water bath. Check periodically by thin layer chromatography, and if the substrate is completely disappeared, after heating for 10 minutes in hot water to complete the reaction, the reaction solution is extracted three times with the same amount of ether, Concentrated. The obtained product was separated by silica gel column chromatography (chloroform: methanol = 9: 1) to obtain a red ginseng extract (yield 10.5%).

  The white birch extract was purchased from Arch Personal Care Products (USA) (trade name: whitebirch extract).

  Papain enzyme was purchased from Refined papain powder manufactured by CPC Wolfgang Muhlbauer GmbH (Germany).

  The licorice extract, red ginseng extract, white birch extract and papain enzyme were mixed at a ratio of 5: 5: 10: 0.3 to produce 203 g of whitening composition (white energy complex) 1 of the present invention ( Example 1).

  Further, the licorice extract, red ginseng extract, white birch extract and papain enzyme were mixed at a ratio of 5: 10: 20: 0.6 to produce 356 g of white energy complex 2 (Example 2).

<Test Example 1> Measurement of inhibitory effect on melanogenesis HM3KO cells (Y. Funasaka, Department of dermatology, Kobe university school of medicine, 5-1 Kusunoki-cho 7-chrome, Chuo-ku, Kobe 650, Japan) was placed in Minimum Essential Medium (MEM) containing 10% fetal bovine serum and cultured under conditions of 37 ° C. and 5% CO ₂ . The cells cultured in this manner were divided into 75-well flasks so that the number of cells was 3 × 10 ⁵ per well, and the cells were waited overnight to attach to the base wall. After confirming that the cells were well attached, the medium was replaced with a new medium containing 10 ppm of each test substance.

Test substances were used as described in Table 1 below.
In the control group, a medium containing only DMSO was used without using a test sample.

  For comparison, all of the licorice extract, kojic acid, arbutin, vitamin A, vitamin C, red ginseng extract, birch extract and papain enzyme used alone and mixed with each other are used. A combination of case numbers was tested.

  The culture was continued until the cells were filled in the flask while changing to a new medium containing the sample once every two days in this manner. Then, after removing the culture solution, washing with PBS, dissolving with 1N sodium hydroxide, measuring the absorbance at 500 nm, and calculating the inhibition rate of the melanin production of the sample with respect to the control group based on the following calculation formula 1. The results are shown in Table 1.

[Calculation Formula 1]
Melanin production inhibition rate (%)
= {1- (absorbance of each test substance / absorbance of control group)} × 100

  From the results in Table 1, the papain, red ginseng extract, and white birch extract that make up the white energy complex have a low inhibition rate of their own melanin production. When used, the whitening effect was increased, and when the licorice extract and all the three components were used together, it was confirmed that the whitening effect was further enhanced.

  Also, in the case of kojic acid, arbutin, vitamin A, and vitamin C used as existing whitening agents, it is confirmed that the whitening effect is further enhanced when papain, red ginseng extract, and birch extract are used together. I was able to.

<Test Example 2> Whitening effect test on human skin After applying an opaque tape with 6 holes of 1.5 cm in diameter to the upper arm of 10 healthy male subjects, Irradiate ultraviolet rays (UVB) 1.5 to 2 times the amount of minimal erythema dose, induce skin darkening, apply test substance, and use chromameter 2 months later The brightness of the skin was measured.

  Each test substance was prepared according to the dosage form examples and comparative dosage form examples shown in Table 2 below, and was applied twice a day (morning and evening) every day.

<Dosage Form Examples 1 and 2 and Comparative Dosage Form Examples 1 to 4>
The following dosage forms are O / W lotion dosage forms, and the unit is% by weight. After manufacturing the following dosage form, it was put into a 30 degreeC thermostat, the viscosity measured on the next day was 7000-11000cp, and pH (10% aqueous solution reference | standard) was 6.5-7.5.

〔Production method〕
(1) Oil phase component 1 was heated (70 to 75 ° C.) and mixed uniformly.
(2) The aqueous phase component 1 was heated (70 to 75 ° C.), and uniformly dissolved and mixed.
(3) The above (2) is stirred while the temperature (70-75 ° C) is maintained while stirring (1) to produce an emulsion, and the oil phase component 2 and the water phase component 2 are continuously charged. And then cooled to 28-30 ° C.

  The effect was determined by obtaining an “L” value indicating the lightness and darkness of the skin (the skin color of a Korean who does not tan generally indicates a value of 50 to 70). When the test substance was applied, the L value gradually increased, and the comparison between the test substances was expressed by ΔL value (final L value−value before applying the test substance). The greater the ΔL value, the greater the whitening effect. The ΔL values based on the experimental results are summarized in Table 3.

  Referring to the experimental results in Table 3, Comparative Formula Type Example 1 using licorice extract alone and Comparative Formula Type Examples 2 to 4 using papain, birch extract and red ginseng extract respectively with licorice extract. Further, in the dosage form examples 1 to 5 using both of the above two components, ΔL appears to be high, and in particular in the dosage form examples 1 and 2 containing the white energy complex using both of the three components, ΔL The value of appears the highest, which indicates that the whitening effect is relatively larger.

  Further, it was confirmed that the larger the amount of the white energy complex, the relatively larger whitening effect was obtained.

  As described above, the cosmetic composition of the oil-in-water emulsion was manufactured using the white energy complex which is the whitening composition of the present invention, and as a result of confirming the whitening enhancement effect, it was confirmed that the whitening effect was enhanced. We were able to.

Claims (4)

A whitening adjuvant composition for whitening activity enhancement of a whitening component, comprising a red ginseng extract, a white birch extract and a papain enzyme .   The composition according to claim 1, wherein the whitening component is selected from the group consisting of kojic acid, arbutin, licorice extract, vitamin A and vitamin C. A whitening cosmetic composition comprising a licorice extract as a whitening component, wherein the composition further contains a red ginseng extract, a white birch extract and a papain enzyme .   The content of each component with respect to the total weight of the cosmetic composition is 0.001 to 2.0% by weight of licorice extract, 0.01 to 0.1% by weight of papain enzyme, 0. The cosmetic composition according to claim 3, wherein the cosmetic composition is 01 to 5.0% by weight and white birch extract 0.01 to 5.0% by weight.