JP4568636B2 - Chitosan-containing green leaf composition and beverage with improved floating and coagulation sedimentation - Google Patents

Description

  The present invention relates to a chitosan-containing green leaf composition improved by using a specific chitosan so that even if chitosan is contained in a green leaf, it hardly causes floating or coagulation precipitation, and particularly relates to a composition suitable for beverages. .

  In recent years, with increasing health consciousness, juice from green leaves of green vegetables such as kale, that is, “green juice” has been eaten by many people. Aojiru is rich in ingredients such as vitamins, minerals and dietary fiber, as well as chlorophylls and flavonoids. It can be expected to improve nutrition and dietary habits of Japanese people who have become westernized and lack vegetables. Vegetables used in green juice are eaten the most by kale, but in recent years they follow kale and are rich in dietary fiber and minerals and have a good taste, such as green leaves of wheat plants such as wheat leaf and barley leaf Aojiru made from the raw material has been eaten. Gramineae plants such as barley (eg, barley and wheat) and rice are typical grains that have been eaten all over the world as staple foods. These green leaves, especially wheat green leaves and barley green leaves, are vitamins and minerals. It is attracting attention as a health food material because it is rich in dietary fiber. The development of green leaf beverages containing chitosan, an animal dietary fiber, has attracted attention for the purpose of imparting further health functions to such health food materials. That is, chitosan is the only basic dietary fiber having an amino group among natural dietary fibers, and when taken together with green juice, health functions can be greatly expected due to the effects of both the green leaf component and chitosan.

For example, when processing green leaves such as chitosan and gramineous plants into beverages, it is generally known that coagulation and sedimentation occurs immediately with the water-soluble components of green leaves and chitosan, significantly reducing the appearance and texture of food. It was. This is due to the fact that chitosan has a polymer coagulation action, as is the case with wastewater treatment and river sludge coagulant. In order to prevent such agglomeration and precipitation, it is conceivable to make the pH alkaline by using sodium bicarbonate or the like. However, these alkaline materials contain sodium and potassium, so they have a salty taste and are not suitable for beverages.
Further, as a method for improving the dispersibility of green leaf fine powder, a method for preventing aggregation and precipitation by adding a polysaccharide such as starch, guar gum or pullulan is also known (see Patent Document 1), which has a temporary effect. However, there is no known method for preventing effective aggregation and precipitation in the combined use of green leaf fine powder and chitosan.
Due to these technical problems, beverages or beverage compositions containing chitosan and green leaves have not been commercialized at present.

Japanese Patent Laid-Open No. 2003-334046

Therefore, the present inventors include both green leaves and chitosan such as green leaves, preferably gramineous plants (more preferably barley), celery plants, and / or convolvulaceae plants, and both have inherent physiological properties. Chitosan-containing green leaves suitable for beverages that have been improved so as not to cause coagulation and precipitation at least until taking, even without adding additives such as pH adjusters and dispersants, without impairing functionality. This research and development was tackled with the goal of developing a composition, particularly a chitosan-containing green leaf composition with a favorable taste and aroma. As a result, a chitosan-containing green leaf composition containing a chitosan having a bulk density of 0.20 to 0.90 g / mL and a particle size of 300 μm or less and a green leaf powder having a particle size of 300 μm or less is used at least. In the meantime, it was found that improvement was made so that almost no coagulation precipitation occurred. However, in subsequent studies, after the composition for use as an aqueous suspension during storage was stored under relatively severe conditions, the composition was dispersed in water, and the aqueous suspension containing both the chitosan and green leaves If the liquid is used, the coagulation sedimentation will be improved, but this time the suspended matter will increase and there will be no problem with drinking, but there is a possibility that the drinker may visually perceive the contamination as an impurity. It turns out to be desirable.
The object of the present invention is to provide a composition suitable for beverages containing chitosan and green leaves, particularly when the composition is used as a water suspension composition for administration after long-term storage. It is an object of the present invention to provide a chitosan-containing green leaf composition that can exist as a uniform dispersion that does not cause agglomeration and precipitation until administration, to the extent that it does not bother much.

  Thus, as a result of various investigations on the cause of the increase in suspended matter, the present inventors have found that the particle size of chitosan is greatly related to the increase in suspended matter, and completed the present invention. That is, according to the study of the present inventors, chitosan having a specific bulk density and particle size, for example, 300 μm, preferably a specific water-treated chitosan particle, more preferably a specific molecular weight (viscosity) chitosan is a green leaf. When suspended in water with fine powder, it exists as a relatively stable water suspension, and the functionality inherent in green leaves and chitosan is not impaired. Although suitable for use, chitosan having a specific range of particle sizes, for example, about 160 μm to 180 μm, is easy to bind to dietary fiber of green leaves, and chitosan bound to this dietary fiber during storage Is found to be the cause of the increase in suspended matter, and that the increase in suspended matter is not observed if the chitosan particle size is about 150 μm or less. It was completed.

That is, the present invention
(1) Water suspension characterized by containing chitosan having a particle diameter of 80% by mass or more and 150 μm (100 mesh) or less and a bulk density of 0.20 to 0.90 g / mL and green leaf powder. Chitosan-containing green leaf composition,
( 2 ) The chitosan-containing green leaf composition for water suspension according to the above (1) , wherein the chitosan is obtained by adding pulverized chitosan to water, then giving motion to the chitosan particles and drying.
( 3 ) The chitosan-containing green leaf composition for water suspension according to the above (1) or (2) , wherein the chitosan content in the chitosan-containing green leaf composition is 1 to 80% by mass,
( 4 ) Chitosan-containing green leaf for water suspension according to (1) or (2) above, wherein the viscosity of chitosan (0.5 W / V% / 0.5% aqueous acetic acid solution, 20 ° C.) is 20 to 400 mPa · s. Composition,
( 5 ) The chitosan-containing green leaf composition for water suspension according to any one of (1) to ( 4 ) above, which is for beverages,
( 6 ) The chitosan-containing green leaf composition for water suspension according to any one of the above (1) to ( 5 ), wherein the remaining chitosan having a particle diameter exceeding 150 μm (100 mesh) has a particle diameter of 300 μm or less,
( 7 ) The water suspension according to any one of (1) to ( 6 ), wherein the green leaf powder is at least one selected from the group consisting of barley green leaf powder, sweet potato stem powder, and Ashitaba stem powder. Chitosan-containing green leaf composition,
About the.

  The chitosan-containing green leaf composition of the present invention contains both the green leaf fine powder and chitosan, and when it is made into an aqueous suspension without using an additive such as a pH adjuster or a dispersant, it is 3 minutes. As described above, the chitosan-containing green leaf composition having a high suspension stability that hardly causes aggregation and precipitation for 10 minutes or more in the more stable one is less liable to be suspended in the water suspension, easy to drink, and used for beverages. It is suitable as a health food.

  The chitosan-containing green leaf composition of the present invention may be either solid or liquid, but is preferably a dry granule preparation or a powder preparation for dissolution at the time of use. In the preferred composition of the present invention for dissolution at the time of use, when the composition is dispersed in water, it hardly flocculates for 3 minutes or more and maintains a suspended dispersion state, and there are few suspended matters, most of which It is possible to maintain a suspended and dispersed state for 10 minutes or more to ensure a state where it is easy to drink.

The chitosan used in the chitosan-containing green leaf composition of the present invention may be chitosan having a particle size of 80% by mass or more of 150 μm (100 mesh) or less, and the rest of the chitosan is preferably 300 μm or less. Further, the chitosan used in the present invention is not particularly limited, but the bulk density is preferably about 0.20 to 0.90 g / ml, preferably about 0.20 to 0.50 g / ml, more preferably. It is about 0.20 to 0.40 g / ml. Such chitosan may be directly pulverized with an appropriate pulverizer such as a jet mill to directly produce chitosan having the above particle diameter. However, considering efficiency and the like, chitosan is usually made to have a particle diameter of 300 μm or less, It is preferably 180 μm (80 mesh) or less and pulverized so that the average particle size is about 80 to 150 μm, more preferably about 100 to 150 μm, and the water for modification described later is used as it is or as necessary. After the treatment, it can be obtained by sieving with a 100-mesh classifier, collecting the material that has passed through 100-mesh, and performing a water treatment for reforming as described later if necessary. In the chitosan thus obtained, 80% by mass or more of chitosan is 150 μm or less.
A more preferable chitosan particle size is 100 μm (150 mesh) or less and an average particle size of 100 to 80 μm is good, and the lower limit of the particle size is not particularly limited, but considering the cost of pulverization, etc., it is usually about 5 μm. . As will be described later, when water treatment of powdered chitosan is carried out for modification, it is sufficient that the particle size and bulk density of the modified chitosan are within the above ranges.

In addition, the chitosan viscosity (0.5 W / V% / 0.5% aqueous acetic acid solution, 20 ° C.) used in the chitosan-containing green leaf composition of the present invention is usually not particularly limited as long as it is 20 mPa · s or more. preferably better than high molecular weight chitosans 100 mPa · s, the upper limit is about 400 mPa · s, usually 350 mPa · s or less, more generally used include: 300 mPa · s. The chitosan used in the present invention having such a viscosity can be obtained by using, for example, chitosan in the above viscosity range as the raw material chitosan for pulverization.

  Any chitosan used in the chitosan-containing green leaf composition of the present invention can be used as long as it has the above particle size and bulk density. Preferably, chitosan powder obtained by a suitable pulverizer such as a jet mill is further used. It is preferable to treat with water for modification. The modification treatment may be carried out by allowing the chitosan powder to contain water and then performing a drying treatment while giving or exercising the exercise. Although the modification mechanism of chitosan powder by the modification treatment is not clear, this modification treatment usually brings the bulk density to an appropriate range, and the suspension stability when the composition of the present invention is made into an aqueous suspension. Is further improved. Probably, this modification treatment is considered to improve the suspension stability by reducing the surface fuzz and the like often found in the pulverized product, thereby improving the suspension smoothly. For example, the reforming treatment is performed by adding 3 parts by mass or more, preferably 5 parts by mass or more of water to 10 parts by mass of pulverized chitosan, adding water to the chitosan, and then mixing them appropriately to give movement to the chitosan particles. Or after feeding, it may be dried. For example, it is preferable to use a conical dryer, a Nauta dryer or the like and dry it while applying rotational motion to the chitosan particles by rotational mixing. Further, the rotational motion and the drying process may be performed separately, for example, the water-containing chitosan is subjected to a rotational motion with a mixing pan or the like and then dried with a shelf dryer.

The amount of water contained in the chitosan is sufficient if it is sufficient to wet the chitosan particles, and even if it is in excess, there is no significant difference in effectiveness. Therefore, from the viewpoint of reducing energy consumption, 10 parts by mass of chitosan is required. On the other hand, it is 50 mass parts or less of water, Preferably it is 40 mass parts or less, More preferably, it is 30 mass parts or less.
The pulverized chitosan (chitosan powder) used for the modification treatment preferably has the particle size and bulk density used in the present invention, but the above-mentioned particle size obtained by a normal jet mill or the like has a bulk density of the present invention. Therefore, there is no particular problem as long as it is treated with water and the treated product has the particle size and bulk density used in the present invention.

  The green leaf powder used in the chitosan-containing green leaf composition of the present invention may be any one obtained by pulverizing green leaves. Examples of the green leaves include green leaves of cereals (for example, barley, wheat, oats, rye and other wheat, rice, whey, straw, millet, corn, sorghum, sugar cane, etc.), asteraceae (for example, Artemisia, etc.), celery family plants (Ashitaba, parsley, celery, etc.), mulberry plants (eg, mulberry), scorpion family plants (eg, Dokudami etc.), perilla plants (eg, perilla), cruciferous plants ( Komatsuna, kale, cabbage, broccoli, etc.), liliaceae plants (eg, asparagus, etc.), lindenaceae plants (eg, Morohoya), convolvulaceae plants (eg, sweet potato foliage), red crustaceae (eg, Green leaves of green plants such as spinach and the like, but are not limited thereto. Preferred green leaves include gramineous plants (preferably barley), celery family plants (preferably Ashitaba), cruciferous plants (preferably kale), convolvulaceae plants (preferably sweet potato foliage) or asteraceae plants (preferably mugwort). More preferred are barley young leaves, kale, sweet potato stover, Ashitaba foliage, mugwort and the like.

  In order to produce a green leaf powder from the green leaves of these plants, the green leaves of these plants (in the present invention, when referred to as green leaves, unless otherwise specified, they are used in a concept including plant stems) are appropriately used. It may be cut into a size, and if necessary, subjected to a discoloration preventing treatment such as a blanching (hot water) treatment, dried if necessary, and then pulverized to an appropriate size. More specifically, for example, in the case of barley green leaves, the green leaves harvested from the beginning of the splitting period to the heading start stage are washed with water and cut into an appropriate length (for example, 10 cm), and then necessary. Accordingly, in order to prevent deterioration of the material (change in green discoloration and flavor), it is subjected to blanching (hot water) treatment, microwave treatment, etc. and dried as necessary, and then, for example, a crusher, It can be obtained by grinding using any machine or tool commonly used by those skilled in the art, such as a mill, blender, or stone mill. From the standpoint of nutrient retention, blanching is preferably a short time. Moreover, when drying, it is preferable that it is freeze-drying or low-temperature heat drying (for example, warm air drying) of 70 degrees C or less. Since the obtained green leaf powder is dried and powdered as it is without squeezing the green leaf, it contains the active ingredient of the green leaf as it is. Moreover, after collecting green leaves, it may be homogenized with a mixer together with a solvent such as water or alcohol, and then dried by hot air drying, freeze drying or spray drying to obtain green leaf powder. The particle size of the green leaf powder used in the chitosan-containing green leaf composition of the present invention is not particularly problematic as long as it is 300 μm or less, but 90% or more of the green leaf powder is preferably 180 μm (80 mesh) or less, More preferably, it is 75 μm (200 mesh) or less. The lower limit of the particle size is not particularly limited, but is usually about 5 μm. And it is preferable that the particle diameter is 300 μm or more, preferably 250 μm or more, more preferably 150 μm or more. Green leaf powder of plants other than barley can also be produced according to the above.

The amount of chitosan in the chitosan-containing green leaf composition in the present invention is not particularly problematic as long as an amount capable of exhibiting the functionality of chitosan is contained. In addition, since the chitosan content varies depending on the dosage amount, dosage form, etc. for a single use, it cannot be said unconditionally. The amount of chitosan therein is usually 1 to 99% by mass, preferably 1 to 80% by mass, more preferably 3 to 70% by mass, and still more preferably 5 to 30% by mass with respect to the total amount of the preparation.
The green leaf powder content in the chitosan-containing green leaf composition in the present invention depends on the amount of the single-use preparation, but there is no problem as long as the nutrient component of the green leaf can be sufficiently ingested. The green leaf powder content depends on the form of the preparation and cannot be generally specified. However, except for the case where the composition is a beverage composition to be drink as it is, it is usually 1 to 99% by mass, preferably 10% with respect to the total amount of the preparation. -60 mass%, More preferably, it is 20-50 mass%.
The content ratio of both chitosan and green leaf powder is not particularly limited, but usually 0.5 to 20 weight percent, preferably 1 to 10 weight percent, more preferably 1 to 5 weight percent of green leaf powder to 1 weight percent of chitosan. It is.
In addition to chitosan and green leaf powder, the chitosan-containing green leaf composition in the present invention includes other additives such as pharmaceutical additives such as carriers, extenders, adhesives, disintegrants, dispersants, etc., and taste modifiers (condiments) Etc.) and the like can be appropriately contained as necessary. The content with respect to the whole preparation is the remaining amount excluding chitosan and green leaf powder, and is usually in the range of 0 to 98%, preferably 0 to 87%, more preferably 0 to 77%.

Are mixed green Hazue and chitosan, tablets and condyle granular, pasty, liquid, granulation according to the gel such purposes, it may be obtained a composition performs formulation process, and the green Hazue Chitosan may be subjected to the above-described formulation treatment alone and mixed to obtain a composition. Moreover, after mixing raw green leaves and chitosan, a dispersant such as water is added and pulverized, and then dried to obtain the chitosan-containing green leaf composition of the present invention. In particular, the chitosan-containing green leaf composition of the present invention pasty, if you gel, since the coagulation and sedimentation does not occur, can be uniformly dispersed paste or gel, dispersing green leaves and chitosan to water Even when wet granulation is performed, a uniform granulated product can be obtained.
Furthermore, since the chitosan-containing green leaf composition of the present invention does not coagulate and precipitate by the time of taking even if it is suspended in water for taking as described above, it is appropriately suspended in water and used as a drink. Suitable for
Such a chitosan-containing green leaf composition does not cause aggregation and precipitation when suspended in water. Therefore, when taken as a dry preparation such as a tablet, the chitosan-containing green leaf composition has improved dispersibility in the oral cavity and gastrointestinal tract. It is also conceivable that the health function of each component in the composition is easily exhibited.

  The water-suspended chitosan-containing green leaf beverage composition according to the present invention is prepared by adding water to the dried green leaf powder, the pulverized chitosan or chitosan powder, and other additives as necessary, or a water suspension as described above. It can be obtained by suspending the chitosan-containing green leaf composition of the present invention in water. Moreover, it can also manufacture by adding the said chitosan or crude chitosan to raw green leaves, other additives and water as required, and homogenizing with a mixer. The procedure for adding chitosan is to mix the components in a dry state, then suspend in water, add chitosan when suspended in water, or adjust the suspension with components other than chitosan, and then Chitosan may be added. The chitosan content in the chitosan-containing green leaf beverage composition comprising the aqueous suspension is not particularly problematic as long as it contains an amount capable of exhibiting the functionality of chitosan, although it depends on the preparation amount for a single use. The chitosan amount is generally 0.05 to 8% (mass: the same unless otherwise specified), preferably 0.1 to 5.0%, and the content of green leaf powder is the nutritional component of green leaves. If it is the quantity which can be ingested enough, there is no problem, and it is usually 0.01 to 40%, preferably 0.1 to 20%, more preferably 0.3 to 10%, and further 0.3 to 5%. The balance is other additives including water, and the water content is at least 30% or more, preferably 52% or more, and 99.94% or less, preferably 99.4% or less, based on the whole preparation. . The remainder is other additives and is 0 to 40% or less.

In the chitosan-containing green leaf composition of the present invention, fragrance, herb, matcha tea, tea, coffee, cocoa, kinako, etc. are added as a fragrance, sugar such as sugar, honey, salt, soy sauce, miso as a seasoning, You can adjust the taste, aroma, and color by adding liquors or even adding pigments. Further, in the formulation, dispersibility and handling in water, in order to improve the filling of the packaging, hard capsules, capsules such as soft capsules, tablets or the pills and the like, or powdery, condyles granular, tea-like, It can also be processed into tea bag, jelly, or cocoon forms, and thickeners (methylcellulose, starch, gum, shellac, etc.) and lubricants (talc, Magnesium stearate, calcium carbonate, eggshell powder, bone powder, shell calcium, dolomite, etc.) can be used. The blending ratio of these additives is not particularly limited, but is preferably 98% or less for a chitosan-containing green leaf composition and 40% or less for a chitosan-containing green leaf beverage. These shapes may be taken by dissolving in water, hot water, milk or the like, and in the case of a tea bag or the like, it may be taken after leaching the components. You can also eat it as you like.

Hereinafter, the present invention will be specifically described with reference to reference examples, examples, and comparative examples, but the present invention is not limited thereto.
Moreover, the modified chitosan used in Reference Examples, Examples and Comparative Examples described later was produced by the methods shown in Table 1-1 and Table 1-2. The viscosity of chitosan was measured by dissolving chitosan in 0.5 W / V% / 0.5% acetic acid aqueous solution and using a bismetholone viscometer VDA type (manufactured by Shibaura System Co., Ltd.) at 20 ° C. did. The particle size was measured by the following method.
Equipment used: Micro type electromagnetic vibration sieve M-2 type (manufactured by Tsutsui Riken Kikai Co., Ltd.)
Sieve: made of stainless steel, 7.5 mm φ sieve blocking inhibitor: Measuring method of glass beads of about 5 mm φ :
1. About 9 g of glass beads are placed on each simple to be used for measurement, and the weight is measured with an accuracy of 0.001 g.
2. Measure the weight of the receiver with an accuracy of 0.001 g.
3. Stack the meshes in the order in which the fine sieve is on the bottom.
4). Place 2.5 g of sample on the top sieve, cover it and vibrate in memory 8 for 10 minutes.
5). Measure the weight of each sieve and receiver.
Subtract the weight of 1 and 2 from the weight of 6.5 to determine the sample weight on the sieve.
The bulk density was measured using a Casa specific gravity measuring instrument (manufactured by Tsutsui Riken Chemical Co., Ltd.), and the sample was placed in a 30 ml stainless steel tube so as not to give vibration, and the excess sample was removed with a spatula. measuring the sample weight at the precision was determined by converting the weight per 1 ml.

(注)＊表中水投入量はキトサンに対する割合

(Note) * The amount of water input in the table is the ratio to chitosan.

(注)＊表中水投入量はキトサンに対する割合
なお、表中、比較例３における粒子径５００μｍ以下は、具体的には粒子径５００〜２００μｍの範囲内で、平均粒子径が４００μｍのものを使用した。

(Note) * The amount of water input in the table is the ratio to chitosan. In the table, the particle size of 500 μm or less in Comparative Example 3 is specifically within the range of 500 to 200 μm and the average particle size is 400 μm. used.

  The modified chitosan (1) to (6), low-density chitosan, low-viscosity chitosan, and coarse-grained chitosan produced in Table 1-1 and Table 1-2 were prepared using chimney and chitosan experiments. In accordance with the method described in the manual (chitin, chitosan study group edited by Gihodo Publishing, 1991), crude chitosan was prepared under the above conditions, washed with water, and then pulverized with a jet mill or the like. At that time, chitosan having a different viscosity was prepared by setting the hydrochloric acid treatment temperature to 10 to 50 ° C. Furthermore, chitosan having a different particle size was prepared by repeating grinding with a jet mill or the like. Further, as a modification treatment that exerts the effects of the present invention, an appropriate amount of water was added to crushed chitosan, and dried at 80 ° C. under reduced pressure using a conical dryer or the like to prepare modified chitosan.

(Preparation example of barley young leaf powder)
The young barley leaves harvested at a height of about 30-40 cm were washed with water to remove the adhering mud and the like. The barley young leaves were cut into about 10 cm, 10 kg of them were put into 100 liters of water, immersed, and heated (branching treatment) at 90 to 100 ° C. for 3 minutes.

Next, the blanched young barley leaves were immediately immersed in cold water at 4 ° C. for 1 minute and cooled. Subsequently, the cooled young barley leaves were centrifuged for 30 seconds for dehydration. The dehydrated young barley leaves were dried in warm air at 60 ° C. for 10 hours in a dryer so that the water content was 5% or less, and then the particle size of 90% by weight or more in each powder was 180 μm by a pulverizer. The barley young leaf powder was crushed to the following (barley young leaf powder (1)) and 75 μm or less (barley young leaf powder (2)).
Moreover, the barley raw young leaf used what washed the water after cutting the said barley.
The particle size of the barley young leaf powder was confirmed to be smaller than each particle size using a laser diffraction / scattering type particle size distribution analyzer (manufactured by Seishin Enterprise Co., Ltd.).

  3 g of barley young leaf powder (1) was collected in a 100 ml stoppered graduated cylinder, and 100 ml of water was added and dispersed and suspended. Thereafter, 1.0 g of modified chitosan (5) was added and suspended for 10 seconds to obtain an aqueous suspension (beverage) of the chitosan-containing green leaf composition of the present invention.

 3 g of barley young leaf powder (2) was collected in a 100 ml stoppered graduated cylinder, and 100 ml of water was added and suspended. Thereafter, 1.0 g of modified chitosan (5) was added and suspended for 10 seconds to obtain an aqueous suspension (beverage) of the chitosan-containing green leaf composition of the present invention.

Reference example 1
Add 10 kg of methylcellulose and 10 kg of water to 10 kg of modified chitosan (1) and young barley leaf powder (2), knead with a speed kneader, dry at 60 ° C. for 5 hours, and then granulate with a speed mill and agglomerate. Improved chitosan-containing green leaf composition.

Reference example 2
300 g of fresh barley leaves, 500 ml of water and 10 g of green tea powder were added to 30 g of the modified chitosan (2) and pulverized with a mixer to obtain a chitosan-containing green leaf drink with improved aggregation precipitation.

Reference example 3
1 kg of methylcellulose and 20 kg of water were added to 38 kg of barley young leaf powder (2), kneaded with a speed kneader, dried at 80 ° C. for 3 hours, and then sized with a speed mill to obtain barley young leaf granules. Further, 10 kg of water was added to 10 kg and dried under reduced pressure at 80 ° C. using a conical dryer to obtain 10 kg of modified chitosan (3) granules. These barley young leaf granules and chitosan granules were mixed in a mass ratio of 3: 1 to obtain a chitosan-containing green leaf composition with improved precipitation.

Reference example 4
10 kg of soluble starch and 30 kg of water are added to 5 kg of modified chitosan (4) and 20 kg of barley young leaf powder (2), kneaded with a speed kneader, granulated with an extrusion granulator, dried at 60 ° C. under reduced pressure for 3 hours and agglomerated A chitosan-containing green leaf composition with improved precipitation was obtained.

Reference Example 5
3 g of barley young leaf powder (1) was collected in a 100 ml stoppered graduated cylinder, and 100 ml of water was added and dispersed and suspended. Thereafter, 1.0 g of modified chitosan (6) was added and suspended for 10 seconds to obtain an aqueous suspension (beverage) of the chitosan-containing green leaf composition of the present invention.

Reference Example 6
3 g of barley young leaf powder (2) was collected in a 100 ml graduated cylinder with a stopper, and 100 ml of water was added and suspended. Thereafter, 1.0 g of modified chitosan (6) was added and suspended for 10 seconds to obtain an aqueous suspension (beverage) of the chitosan-containing green leaf composition of the present invention.

Reference Example A-1
3 g of barley young leaf powder (1) was collected in a 100 ml stoppered graduated cylinder, and 100 ml of water was added and dispersed and suspended. Thereafter, 1.0 g of low-density chitosan was added and suspended for 10 seconds to obtain an aqueous suspension (beverage) of a chitosan-containing green leaf composition for comparison.

Reference Example A-2
3 g of barley young leaf powder (1) was collected in a 100 ml stoppered graduated cylinder, and 100 ml of water was added and dispersed and suspended. Thereafter, 1.0 g of low-viscosity chitosan was added and suspended for 10 seconds to obtain an aqueous suspension (beverage) of a chitosan-containing green leaf composition for comparison.

Reference Example A-3
3 g of barley young leaf powder (1) was collected in a 100 ml stoppered graduated cylinder, and 100 ml of water was added and dispersed and suspended. Thereafter, 1.0 g of coarse chitosan was added and suspended for 10 seconds to obtain an aqueous suspension (beverage) of a chitosan-containing green leaf composition for comparison.

Reference Example A-4
3 g of barley young leaf powder (2) was collected in a 100 ml graduated cylinder with a stopper, and 100 ml of water was added and suspended. Thereafter, 1.0 g of low density chitosan was added and suspended for 10 seconds to obtain an aqueous suspension (beverage) of a comparative chitosan-containing green leaf composition.

Reference Example A-5
3 g of barley young leaf powder (2) was collected in a 100 ml graduated cylinder with a stopper, and 100 ml of water was added and suspended. Thereafter, 1.0 g of low-viscosity chitosan was added and suspended for 10 seconds to obtain an aqueous suspension (beverage) of a comparative chitosan-containing green leaf composition.

Reference Example A-6
3 g of barley young leaf powder (2) was collected in a 100 ml graduated cylinder with a stopper, and 100 ml of water was added and suspended. Thereafter, 1.0 g of coarse chitosan was added and suspended for 10 seconds to obtain an aqueous suspension (beverage) of a comparative chitosan-containing green leaf composition.

Test example 1
Using the aqueous suspension (beverage) of the chitosan-containing green leaf composition obtained in Example 1, Example 2, Reference Examples 5 and 6, and Reference Examples A-1 to 6, coagulation precipitation was performed using the precipitation occurrence time as an index. The improvement effect was evaluated. Each beverage was allowed to stand immediately after preparation. Each suspension component was separated by coagulation sedimentation, and the time required for the clear layer to be 5 ml or more was measured. The measurement results are shown in Table 2. Judgment was made good when the product was distributed as a product in consideration of the time for the consumer to drink and drink the young leaf beverage, and the product that did not cause coagulation sedimentation for 10 minutes or more was judged good, and the product that produced coagulation sedimentation and the appearance was impaired was regarded as poor.

Table 2. Coagulation sedimentation time when modified chitosan barley young leaf beverage is added

 As is clear from the results in Table 2, after pulverized chitosan having a density of 0.20 to 0.90 g / ml and a particle diameter of 300 μm to 5 μm was impregnated with water, the chitosan particles were subjected to motion and dried. The chitosan-containing barley young leaf beverage having a barley young leaf powder particle size of 300 μm to 5 μm has an improved sedimentation time of 10 minutes or more, and a homogenized beverage was obtained. Moreover, about the thing of Example 1 and 2 of this invention, it fills and seals the obtained composition to an aluminum pouch 3g at a time similarly to Example 4 mentioned later, and prepares the package of a total of 5 packages, and test When evaluated in the same manner as in Example 2, the suspended matter was as small as 200 or less.

  The water suspension of the chitosan-containing green leaf composition of the present invention was used in the same manner as in Example 1, except that dry powder of Ashitaba (particle size of 180 μm or less) was used instead of the young barley powder (1) of Example 1. A suspension (beverage) was prepared. Using this, the coagulation sediment improvement effect was evaluated in the same manner as in Test Example 1. Moreover, the water suspension (beverage) of the chitosan containing green leaf composition of a present Example was confirmed visually. As a result, there was no suspended solids, the precipitation occurrence was improved to 10 minutes or more, and a homogenized beverage was obtained.

The modified chitosan (6) was passed through an electromagnetic vibration sieve and the chitosan passing through 100 mesh was recovered to obtain a modified chitosan (7) (average particle size of 80 to 100 μm). When this modified chitosan (7) was applied again to an electromagnetic vibration sieve, chitosan passing through 100 mesh was 80% by mass or more.
Then, 30 kg of barley young leaf powder (1) and 10 kg of modified chitosan (7) were mixed and subjected to fluidized bed granulation to obtain a chitosan-containing green leaf composition of the present invention. 3 g of the obtained composition was filled in an aluminum pouch and sealed, to prepare a total of 5 sachets.

  A package filled with the chitosan-containing green leaf composition of the present invention was obtained in the same manner as in Example 4 except that the dried powder of Ashitaba of Example 3 was used instead of the young barley powder (1) of Example 4. It was.

Comparative Example 1
In the same manner as in Example 4 except that modified chitosan (6) (average particle size 120 to 140 μm) was used instead of modified chitosan (7), a package filled with the chitosan-containing green leaf composition was prepared. Obtained.

Comparative Example 2
A package filled with the chitosan-containing green leaf composition was obtained in the same manner as in Example 4 except that modified chitosan (6) was used instead of modified chitosan (7).

Test example 2
Using the sachets obtained in Examples 4 and 5 and Comparative Examples 1 and 2, the floating improvement effect was evaluated as follows. First, after each sachet was stored at 55 ° C. for 13 days, the composition in the sachet was mixed with water so as to be 200 ml per sachet and allowed to stand for 1 minute. Subsequently, the particles of chitosan floating in a white lump were counted. The results are shown in Table 3. The values in the table are average values.

Table 3 Floating improvement effect of modified chitosan when added to green leaf beverage

  From the results shown in Table 3, the chitosan-containing green leaf composition containing the modified chitosan of the present invention has an excellent chitosan-containing green leaf composition suitable for beverages because the suspended matter that occurs when suspended in water is extremely small even after storage. It turns out that it is a thing. From this, it was found that the chitosan-containing green leaf composition of the present invention can be suitably used for beverages because a beverage with improved floating and coagulation sedimentation is obtained when suspended in a solvent such as water.

The chitosan-containing green leaf composition of the present invention is obtained in the same manner as in Example 4 except that dry powder of sweet potato stems and leaves (particle size is 180 μm or less) is used instead of the barley young leaf powder (1) in Example 4. It was. The obtained composition was filled in aluminum pouches in an amount of 3 g each in the same manner as in Example 4 and sealed, and a total of 5 sachets were prepared and evaluated in the same manner as in Test Example 2. As a result, suspended matter was 200 or less. There were few things. In this example, the same result was obtained even when a mugwort dry powder (particle diameter of 180 μm or less) was used instead of the sweet potato stover.

Claims (7)

Contains chitosan for water suspension, characterized in that it contains chitosan having a particle size of 80% by mass or more and 150 μm (100 mesh) or less and a bulk density of 0.20 to 0.90 g / mL and green leaf powder Green leaf composition. The chitosan-containing green leaf composition for water suspension according to claim 1 , wherein the chitosan is obtained by adding pulverized chitosan to water and then imparting motion to the chitosan particles and drying. The chitosan-containing green leaf composition for water suspension according to claim 1 or 2 , wherein the chitosan content in the chitosan-containing green leaf composition is 1 to 80% by mass. The chitosan-containing green leaf composition for water suspension according to claim 1 or 2 , wherein the chitosan has a viscosity (0.5 W / V% / 0.5% acetic acid aqueous solution, 20 ° C) of 20 to 400 mPa · s. It is for drinks, The chitosan containing green leaf composition for water suspension as described in any one of Claims 1-4 . The chitosan-containing green leaf composition for water suspension according to any one of claims 1 to 5 , wherein the remaining chitosan having a particle diameter exceeding 150 µm (100 mesh) has a particle diameter of 300 µm or less. The green leaf composition for water suspension according to any one of claims 1 to 6 , wherein the green leaf powder is at least one selected from the group consisting of barley green leaf powder, sweet potato stem powder and Ashitaba stem powder.