JP4448927B2 - Solution for ectoparasite control of animals - Google Patents

Description

【００１７】
［実施例１］
上記のように製造した外部寄生虫駆除用液剤である製剤例１、２、４、５、６、７の外部寄生虫駆除用液剤を用いて以下の試験を行った。
【００１８】
［駆除効果試験］
ネコ（アメリカンショートヘアー、２〜３才、雌、体重５ｋｇ）に１頭当り１００匹のネコノミ成虫を接種し、２４時間順化させた後、上記で得られた外部寄生虫駆除用液剤を各々、ネコの背中被毛をかき分けて皮膚に直接滴下した。滴下は、以下に示す量を４等分し、スポイトを用いて４箇所に行なった。
滴下量：製剤例１、４、５、６および７は、２ｍｌ／頭
製剤例２は、２．５ｍｌ／頭
上記試験結果は、以下の▲１▼〜▲３▼のように判定し、これらの結果を表２に示した。
▲１▼ 寄生数：外部寄生虫駆除用液剤の滴下後、４８時間後にノミ取り櫛を用いて毛を梳いて、梳き取られたノミの数を数え、表中に以下の記号で表した。
− ８０〜１００匹
＋ ４０〜 ７９匹
＋＋ ６〜 ３９匹
＋＋＋ ０〜 ５匹
▲２▼ 産卵数：ネコの飼育箱の下にトレイを設けておき、この中に産下されたノミの卵を補集し、数え、表中に以下の記号で表した。
− ５０〜 ７０個
＋ ３０〜 ４９個
＋＋ ６〜 ２９個
＋＋＋ ０〜 ５個
▲３▼ 孵化数：上記の▲２▼で補集された卵を２５℃、湿度８０％で濾紙上に保存し、その孵化状況を観察し、孵化率（％）を計算した。
− ８０％以上
＋ ４０〜 ７９％
＋＋ ６〜 ３９％
＋＋＋ ０〜 ５％
供試動物（ネコ）に対する副作用
− 全く異常が認められない。
＋ 活発でなくなり、少し異常が認められる。
＋＋ 脚がふらつく等の異常が認められる。
【００１９】
【表２】

【００２０】
表２の結果からも明らかなように、この発明の外部寄生虫駆除用液剤は、供試動物であるネコに対する副作用がなく、ノミの寄生数、産卵数を減少させ、さらに孵化率を低下させることにより次世代のノミの発生を抑制することにより、効果的にノミを駆除することができた。
【００２１】
［実施例２］
ネコ（フレンチドメステイク、２才、雌、体重３ｋｇ）に上記の製剤例３、７および８の外部寄生虫駆除用液剤をネコの背中被毛をかき分けて皮膚に直接滴下した。滴下は、外部寄生虫駆除用液剤１．５ミリリットルを３等分し、スポイトを用いて３箇所に行なった。１日後、１週間後、２週間後および３週間後、上記の液剤で処理したネコに対して、ネコ１頭当りネコノミの成虫３０匹を接種した。各接種３日後に、ノミ取り櫛を用いてネコ毛を梳いて、梳き取られたノミの数を数え、ネコノミの寄生数を調査し、寄生率を求めた。
【００２２】
【数１】

【００２３】
実施例２の試験結果を表３に示した。
【００２４】
【表３】

【００２５】
表３の結果からも明らかなように、この発明の外部寄生虫駆除用液剤は、長期に亘ってネコノミのネコへの寄生を阻害することができた。
【００２６】
［実施例３、比較例１］
この発明に係る外部寄生虫駆除用液剤の動物の体表面での薬剤の広がり易さを示すため、以下のモデル実験を行った。
【００２７】
ｄ−フェノトリンとピリプロキシフェンをジエチレングリコールモノエチルエーテルおよびジプロピレングリコールモノメチルエーテルのそれぞれに溶解し、ｄ−フェノトリンの濃度が４０％、ピリプロキシフェンが０．８％となる溶液を調製した。ジエチレングリコールモノエチルエーテル溶液を「試験溶液（実施例３）」、ジプロピレングリコールモノメチルエーテル溶液を「対照溶液（比較例1）」とする。
【００２８】
鞣し、乾燥させたイヌの皮を用意し、この皮の表側に「試験溶液（実施例３）」と「対照溶液（比較例1）」をピペットで１滴（約２５ｍｇ）滴下し、液が完全に皮に染み込むまでの時間を測定した。なお、滴下位置は互いに近接させ、滴下は同時に行った。次に、滴下位置を入れ替えて再度滴下実験を行い、２回の平均値をとって染み込み時間とした。実施例３の実験結果を表４に示した。
【００２９】
【表４】

【００３０】
表４の結果からも明らかなように、ｄ−フェノトリンとピリプロキシフェンをジエチレングリコールモノエチルエーテルに溶解した溶液は、対照のジプロピレングリコールモノメチルエーテルを用いた場合に比べ、動物の皮膚に対するしみ込み時間が短く、それだけ体表面での液の広がりも速いことがわかる。
【００３１】
【発明の効果】
この発明の動物の外部寄生虫駆除用液剤は、以上説明したように、所定の有効成分を所定の溶媒に溶解したので、動物の外部寄生虫を確実に駆除できるものになり、しかも施用される動物に対して副作用の少ない外部寄生虫駆除用液剤であるという利点がある。
【００３２】
また、この発明の動物の外部寄生虫駆除方法は、上記の外部寄生虫駆除用液剤を効率よく使用する方法であるから、動物の外部寄生虫を確実に駆除でき、かつ動物に対する副作用が少ない方法であるといえる。[0001]
BACKGROUND OF THE INVENTION
The present invention relates to an animal ectoparasite-controlling agent and an animal ectoparasite to control ectoparasites parasitic on the body surface of domestic animals and poultry such as pets such as dogs and cats, cattle, pigs and chickens. It relates to the removal method.
[0002]
[Background Art and Problems to be Solved by the Invention]
Conventionally, various ectoparasite-controlling agents and various control methods using them have been employed to prevent damage to animals such as pets, livestock, and poultry by fleas and ticks.
[0003]
However, pesticides are not satisfactory because they have problems such as side effects on animals and their efficacy is not always sufficient.
[0004]
JP-A-11-5702 discloses a preparation example in which pyriproxyfen and phenothrin are added to dipropylene glycol monomethyl ether. This preparation is applied to the neck of an animal (mouse), and then cold water is added. It has been described that the efficacy is greatly reduced when showered. Such a pesticide has a poor diffusion of a chemical solution on the body surface of the animal, and it cannot reliably eliminate the ectoparasite of the animal.
[0005]
Accordingly, an object of the present invention is an ectoparasite-controlling solution that solves the above-mentioned problems of the prior art and can surely control the ectoparasites of animals, and has few side effects on the applied animals. The solution for parasite control and ectoparasite control method.
[0006]
[Means for Solving the Problems]
In order to solve the above problems, in the present invention, 3-phenoxybenzyl 2,2-dimethyl-3- (2-methylprop-1-enyl) cyclopropanecarboxylate and 4 are used as a solution for controlling ectoparasites of animals. -Phenoxyphenyl 2- (2-pyridyloxy) propyl ether was used as an active ingredient, and a solution prepared by dissolving these active ingredients in diethylene glycol monomethyl ether or diethylene glycol monoethyl ether was employed.
[0007]
Alternatively, in order to solve the above-mentioned problems, in the present invention, as a method for controlling ectoparasites of animals, 3-phenoxybenzyl 2,2-dimethyl-3- (2-methylprop-1-enyl) cyclopropanecarboxylate and -Phenoxyphenyl 2- (2-pyridyloxy) propyl ether as an active ingredient, and a solution prepared by dissolving these active ingredients in diethylene glycol monomethyl ether or diethylene glycol monoethyl ether is provided and dripped onto the animal body surface. The ectoparasite control method was adopted.
[0008]
DETAILED DESCRIPTION OF THE INVENTION
Animals to be applied subject to external parasite extermination solutions of the present invention, Ru dog or cat der.
[0009]
Further, examples of ectoparasites to be controlled include fleas such as dog fleas, cat fleas, and human fleas, and ticks such as lice, ticks, tsutsugamushi, sasaradon, hymenid mites, and black tick mites. In addition, in the present invention, ectoparasites such as mosquitoes, fly flies, flies, and flyfish that do not always live on the surface of the animal's body but cause harm by blood sucking from the animal's body surface are also included .
[0010]
There are various stereoisomers of 3-phenoxybenzyl 2,2-dimethyl-3- (2-methylprop-1-enyl) cyclopropanecarboxylate used in the present invention. Stereoisomers or mixtures of the stereoisomers are used, for example 3-phenoxybenzyl (1R) -cis, trans-2,2-dimethyl-3- (2-methylprop-1-enyl) cyclopropanecarboxylate ( Hereinafter referred to as d-phenothrin).
[0011]
The solution for ectoparasite control of the present invention is usually 1 to 50% by weight of 3-phenoxybenzyl 2,2-dimethyl-3- (2-methylprop-1-enyl) cyclopropanecarboxylate and 0 of pyriproxyfen. 0.05 to 5% by weight, and further 20 to 98.95% by weight of diethylene glycol monomethyl ether or diethylene glycol monoethyl ether . Also, other solvents such as alcohols such as propylene glycol, ethanol, isopropyl alcohol and benzyl alcohol, hydrocarbons such as kerosene, esters such as propylene carbonate, soybean oil, coconut oil, palm oil, linseed oil, cottonseed oil, rapeseed oil , Vegetable oils such as tung oil, castor oil, olive oil and rice bran oil may be further contained.
[0012]
Examples of the diethylene glycol monoalkyl ether used in the present invention include C1-C4 alkyl ethers such as diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, and diethylene glycol monobutyl ether. In particular, it is preferable to use diethylene glycol monomethyl ether or diethylene glycol monoethyl ether as the diethylene glycol monoalkyl ether in the present invention.
[0013]
Furthermore, the liquid for ectoparasite control of this invention may contain a synergist, a stabilizer, a fragrance | flavor, etc. Examples of the synergist include N- (2-ethylhexyl) -1-isopropyl-4-methylbicyclo [2.2.2] oct-5-ene-2,3-dicarboximide, N- (2- Ethylhexyl) bicyclo [2.2.1] hept-5-ene-2,3-dicarboximide (abbreviated as MGK-264), isobornyl thiocyanoacetate (abbreviated as IBTA), piperonylbutoxy Examples of the stabilizer include butylhydroxytoluene (abbreviated as BHT), butylhydroxyanisole (abbreviated as BHA), 4,4′-thiobis (3-methyl-6-), and the like. t-butylphenol) (abbreviated as TBMTBP). Examples of the fragrances include terpenes or terpene derivatives such as citronellal, citronellol, and limonene.
[0014]
The ectoparasite-controlling solution of the present invention is usually applied to the animal body surface by dropping, coating, spraying, etc., but it is sufficiently effective simply by dropping it on one or several places on the animal body surface. is there. The application amount is generally the total amount of 3-phenoxybenzyl 2,2-dimethyl-3- (2-methylprop-1-enyl) cyclopropanecarboxylate and pyriproxyfen, which are active ingredient compounds, and the body weight of the animal is 1 kg. It is 3 to 350 mg per unit, preferably 15 to 350 mg.
[0015]
【Example】
Hereinafter, the present invention will be described in more detail with reference to examples.
Formulation Examples 1-5
Each component was uniformly mixed at a mixing ratio (% by weight) shown in Table 1 to produce a solution for ectoparasite control according to the present invention.
[0016]
[Table 1]

[0017]
[Example 1]
The following tests were carried out using the ectoparasite-controlling agents of Formulation Examples 1, 2, 4, 5, 6, and 7, which are liquids for ectoparasite control manufactured as described above.
[0018]
[Disinfection effect test]
Cats (American short hair, 2-3 years old, female, 5 kg body weight) were inoculated with 100 cat flea adults per head, allowed to acclimatize for 24 hours, and then each of the ectoparasite control solutions obtained above was used. The cat's back coat was scraped and dropped directly onto the skin. The dropping was performed by dividing the amount shown below into four equal parts and using a dropper at four locations.
Dropping amount: Formulation examples 1, 4, 5, 6 and 7 are 2 ml / head formulation example 2 is 2.5 ml / head The above test results are determined as in the following (1) to (3). The results are shown in Table 2.
{Circle around (1)} Parasitic number: After dripping the liquid for ectoparasite control, 48 hours later, the hair was combed using a flea removal comb, the number of fleas scraped off was counted, and the following symbols were used in the table.
-80-100 animals + 40-79 animals +++ 6-39 animals +++ 0-5 animals (2) Number of eggs: A tray is placed under the cat's breeding box, and the flea eggs laid in this are placed in the tray. Collected, counted, and represented by the following symbols in the table.
-50-70 + 30-49 +++ 6-29 +++ 0-5 5 (3) Number of hatched eggs: Store the eggs collected in (2) above on filter paper at 25 ° C and 80% humidity. The hatching rate was observed and the hatching rate (%) was calculated.
-80% or more + 40-79%
++ 6-39%
++++ 0-5%
Side effects on test animals (cats)-No abnormalities are observed.
＋ It becomes inactive and some abnormalities are recognized.
++ Abnormalities such as leg swings are observed.
[0019]
[Table 2]

[0020]
As is clear from the results in Table 2, the ectoparasite-controlling solution of the present invention has no side effects on the test animal, reduces the number of flea parasites and the number of eggs laid, and further reduces the hatching rate. By controlling the generation of next-generation fleas, it was possible to effectively eliminate fleas.
[0021]
[Example 2]
A cat (French domestic stake, 2 years old, female, body weight 3 kg) was directly dropped onto the skin by applying the ectoparasite-controlling solution of Formulation Examples 3, 7 and 8 to the back of the cat. The dripping was carried out at 3 locations using a dropper by dividing 1.5 ml of the ectoparasite-controlling solution into 3 equal parts. One day, one week, two weeks, and three weeks later, 30 cat fleas per cat were inoculated to the cats treated with the above solution. Three days after each inoculation, the cat hair was combed using a flea removal comb, the number of fleas scraped off was counted, the number of cat flea infestations was investigated, and the parasitic rate was determined.
[0022]
[Expression 1]

[0023]
The test results of Example 2 are shown in Table 3.
[0024]
[Table 3]

[0025]
As is clear from the results in Table 3, the ectoparasite-controlling solution of the present invention was able to inhibit the cat flea infestation over a long period of time.
[0026]
[Example 3, Comparative Example 1]
In order to show the ease of spreading of the ectoparasite-controlling agent according to the present invention on the surface of the animal body, the following model experiment was conducted.
[0027]
d-Phenotoline and pyriproxyfen were dissolved in diethylene glycol monoethyl ether and dipropylene glycol monomethyl ether, respectively, to prepare a solution having a d-phenothrin concentration of 40% and a pyriproxyfen concentration of 0.8%. The diethylene glycol monoethyl ether solution is referred to as “test solution (Example 3)”, and the dipropylene glycol monomethyl ether solution is referred to as “control solution (Comparative Example 1)”.
[0028]
Prepare and dry the skin of the dog, and drop 1 drop (about 25 mg) of “Test Solution (Example 3)” and “Control Solution (Comparative Example 1)” on the front side of the skin. The time until the skin completely penetrated was measured. The dropping positions were close to each other and dropping was performed simultaneously. Next, the dropping position was changed and the dropping experiment was performed again, and the average value of two times was taken as the soaking time. The experimental results of Example 3 are shown in Table 4.
[0029]
[Table 4]

[0030]
As is apparent from the results in Table 4, the solution of d-phenothrin and pyriproxyfen dissolved in diethylene glycol monoethyl ether has a longer penetration time into the animal's skin than when the control dipropylene glycol monomethyl ether was used. It is clear that the spread of the liquid on the body surface is fast.
[0031]
【The invention's effect】
As described above, the solution for animal ectoparasite control according to the present invention dissolves a predetermined active ingredient in a predetermined solvent, so that it can reliably control the animal ectoparasite and is applied. There is an advantage that it is a liquid for ectoparasite control with less side effects on animals.
[0032]
The animal ectoparasite extermination method of the present invention is a method of efficiently using the ectoparasite extermination solution described above, so that it is possible to reliably eliminate animal ectoparasites and has few side effects on animals. You can say that.

Claims (2)

3-phenoxybenzyl 2,2-dimethyl-3- (2-methylprop-1-enyl) cyclopropanecarboxylate and 4-phenoxyphenyl 2- (2-pyridyloxy) propyl ether as active ingredients, and these active ingredients are diethylene glycol A solution for controlling ectoparasites in dogs or cats dissolved in monomethyl ether or diethylene glycol monoethyl ether. 3-phenoxybenzyl 2,2-dimethyl-3- (2-methylprop-1-enyl) cyclopropanecarboxylate and 4-phenoxyphenyl 2- (2-pyridyloxy) propyl ether as active ingredients, and these active ingredients are diethylene glycol A method for controlling ectoparasites in dogs or cats , in which a solution prepared by dissolving in monomethyl ether or diethylene glycol monoethyl ether is provided and dripped onto the dog or cat body surface .