JP4431783B2 - Process for producing processed food of rice having active oxygen scavenging ability and food produced thereby - Google Patents

Process for producing processed food of rice having active oxygen scavenging ability and food produced thereby Download PDF

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JP4431783B2
JP4431783B2 JP2003429976A JP2003429976A JP4431783B2 JP 4431783 B2 JP4431783 B2 JP 4431783B2 JP 2003429976 A JP2003429976 A JP 2003429976A JP 2003429976 A JP2003429976 A JP 2003429976A JP 4431783 B2 JP4431783 B2 JP 4431783B2
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active oxygen
oxygen scavenging
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美展 秋山
長範 大久
真木夫 高橋
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Description

この発明は、米粉に、大豆粉又は茶(例えば緑茶)粉末などを混入し、調理し又はリン酸基を有する物質を添加して得た活性酸素消去能を有する米の加工食品及び活性酸素消去能が高められた食品並びにその製造方法に関する。   The present invention relates to a processed rice food having active oxygen scavenging ability and active oxygen scavenging obtained by mixing soybean powder or tea (for example, green tea) powder with rice flour, cooking, or adding a substance having a phosphate group. The present invention relates to a food with enhanced performance and a method for producing the same.

活性酸素は癌や生活習慣病の原因物質として注目されている。この活性酸素を消去することのできる成分が穀類や豆類に含まれていることが知られている(Luminesence,16,237−241,2001)。また米と大豆、米と茶を混合すると活性酸素を消去する力が飛躍的に高くなることを発明者らは発見し、その詳細について調べてきた(Proceedings of the 3rd international soybean processing and utilization conference,183−184,2000)。相乗効果発現に関与する成分は、米や雑穀類のサイアミン、イノシトール6リン酸と茶のカテキン、大豆のイソフラボンであった。   Active oxygen has attracted attention as a causative substance of cancer and lifestyle-related diseases. It is known that cereals and beans contain a component capable of eliminating the active oxygen (Luminesence, 16, 237-241, 2001). In addition, the inventors have discovered that mixing of rice and soybeans and rice and tea dramatically increases the ability to eliminate active oxygen, and have investigated the details thereof (Proceedings of the 3rd international soybean processing and utility conference, 183-184, 2000). Ingredients involved in the expression of synergistic effects were thiamine in rice and millet, inositol 6-phosphate and tea catechin, and isoflavone in soybean.

また、全重量に対して60重量%〜80重量%のはと麦と、大豆、小麦胚芽及び米ぬか及び/又は玄米胚芽からなり、かつ夫々を7.5〜27.5重量%とした主成分を、50℃〜150℃で蒸すか、又は50℃〜150℃で焙煎した後に蒸し、しかる後糀かび酵母、イースト菌のうち少なくとも一つを加えて発酵させ、発酵終了後は20〜30重量%のごま粉末及び10〜20重量%の緑茶粉末を加えた活性酸素抑制組成分が知られている。   Moreover, the main component which consists of bean wheat, soybeans, wheat germ and rice bran, and / or brown rice germ of 60 to 80% by weight with respect to the total weight, and each 7.5 to 27.5% by weight Steamed at 50 ° C. to 150 ° C. or roasted at 50 ° C. to 150 ° C. and then steamed, and then fermented with at least one of Aspergillus oryzae yeast and 20-30 wt. An active oxygen inhibiting composition containing 10% by weight sesame powder and 10-20% by weight green tea powder is known.

活性酸素はがんや生活習慣病の原因物質として注目されており、生体内で過剰に発生した活性酸素が前記の疾病を誘導するものと考えられている。この活性酸素の毒性を消去することのできる一群の物質が活性酸素消去物質と呼ばれており、穀類をはじめ野菜、果実等に含まれていることが明らかになっている。活性酸素消去物質としては、ポリフェノール、フラボノイドなどが知られている。
特許第2610325号
Active oxygen is attracting attention as a causative substance of cancer and lifestyle-related diseases, and it is considered that active oxygen generated excessively in the body induces the above-mentioned diseases. A group of substances capable of eliminating the toxicity of active oxygen is called active oxygen-eliminating substances and has been found to be contained in cereals, vegetables, fruits and the like. As active oxygen scavenging substances, polyphenols, flavonoids and the like are known.
Japanese Patent No. 2610325

前記従来の技術を利用して食品を加工する場合には、活性酸素消去能が著しく不安定になる問題点があった。   When food is processed using the conventional technique, the active oxygen scavenging ability becomes extremely unstable.

前記不安定要因を研究した結果、例えば大豆と米との組み合わせでプリン等の加工食品を製造する際の加熱工程が、前記活性酸素消去能を破壊し、不安定要因になることが判明した。   As a result of studying the instability factor, it has been found that, for example, a heating step in producing processed foods such as pudding with a combination of soybeans and rice destroys the active oxygen scavenging ability and becomes an instability factor.

前記プリンに拘わらず、加工食料品について同様の問題点があった。   Despite the pudding, there were similar problems with processed food products.

また、前記活性酸素消去物質は、多くの場合苦みや渋みを有することから、これら物質を多量に含有させると食品の嗜好性が著しく低下するという問題があった。   Further, since the active oxygen scavenging substance often has bitterness and astringency, there is a problem that the palatability of the food is significantly reduced when a large amount of these substances are contained.

この発明は、活性酸素消去能を有する材料を用い、これを比較的低い調理温度で調理し、又はリン酸基を有する物質を添加することにより、前記従来の問題点を解決したのである。   The present invention solves the above-mentioned conventional problems by using a material having an active oxygen scavenging ability, cooking it at a relatively low cooking temperature, or adding a substance having a phosphate group.

即ちの発明は、玄米粉に対し、大豆粉、豆乳又は茶粉末の1種又は2種を20%〜50%(重量)容器に投入し、これに水を加え、又は水を加えることなくこれを撹拌混合して混合液を生成し、この混合液を60℃〜90℃の温度で30分〜50分間加熱することを特徴とした活性酸素消去能を有する米の加工食品の製造方法である。 That this invention, to brown rice flour, soy flour, was placed in one or the 20% to 50% (by weight) container soymilk or tea powder, water was added thereto, or without the addition of water This is stirred and mixed to produce a mixed solution, and this mixed solution is heated at a temperature of 60 ° C. to 90 ° C. for 30 minutes to 50 minutes. is there.

次に他の発明は、請求項1記載の製造方法で製造したことを特徴とする活性酸素消去能を有する食品である。 Next, another invention is a food having active oxygen scavenging ability, which is produced by the production method according to claim 1 .

前記発明において、大豆と米、又は米と緑茶の組み合わせが、米、大豆、緑茶の有する活性酸素消去能を飛躍的に増大させることが判明した。   In the said invention, it turned out that the combination of soybean and rice, or the combination of rice and green tea dramatically increases the active oxygen scavenging ability of rice, soybean and green tea.

例えば、米と大豆の等量組み合わせにおいては、玄米又は大豆の本来所有する活性酸素消去能の4倍以上の相乗効果があることが判明した。   For example, it has been found that an equal amount combination of rice and soybean has a synergistic effect that is at least four times the active oxygen scavenging ability originally owned by brown rice or soybean.

また、米と緑茶との等量の組み合わせにおいても、単独使用の場合に比し、3倍程度の相乗効果が確認された。   In addition, even in a combination of equal amounts of rice and green tea, a synergistic effect of about 3 times was confirmed as compared with the case of using alone.

この発明は、前記相乗効果を利用すると共に、食品調理における活性酸素消去能の減少を防止し、その安定化を図ったものである。   The present invention utilizes the above-mentioned synergistic effect and prevents the reduction of the active oxygen scavenging ability in food cooking and stabilizes it.

またリン酸基を有する物質が前記活性酸素消去物質と共存するとき、その活性酸素消去能が2倍から5倍程度まで増強されるという知見に基づいてなされた。すなわち、前記活性酸素消去物質にサイアミン、又はイノシトールのリン酸誘導体を添加することにより、活性酸素消去能が相乗的に増強された食品となった。   Moreover, when the substance which has a phosphate group coexists with the said active oxygen scavenging substance, it was made | formed based on the knowledge that the active oxygen scavenging ability was enhanced from about 2 times to about 5 times. That is, by adding a thiamine or a phosphoric acid derivative of inositol to the active oxygen scavenging substance, a food in which the active oxygen scavenging ability was synergistically enhanced was obtained.

活性酸素は、人体細胞を損傷するので、老化の一原因とも言われており、その改善により、疲労回復能力、筋力の衰え、体のこりや痛みの改善、機能低下を防止できると言われている。   Active oxygen is also said to be a cause of aging because it damages human body cells, and its improvement is said to prevent fatigue recovery ability, muscle weakness, improvement of body stiffness and pain, and functional decline .

前記老化現象のみならず、関節リューマチ、動脈硬化、脳卒中、心筋梗塞、糖尿病などの各種疾患の原因の一つとも言われている。   It is said to be one of the causes of various diseases such as rheumatoid arthritis, arteriosclerosis, stroke, myocardial infarction and diabetes, as well as the aging phenomenon.

前記活性酸素は、人体内で生体異物を溶解させるために、生体防御上必要な物質であるが、過剰生産により、人体細胞を損傷するおそれがある。   The active oxygen is a substance necessary for biological defense in order to dissolve xenobiotics in the human body, but there is a possibility that human cells are damaged by overproduction.

そこで人体は、過剰生産された活性酸素を除去して、生体を防御するために、スーパーオキシドジスムターゼ(以下、SODという)が細胞の内部で生成され、バランスをとって、人体の健康を確保している。   Therefore, in order for the human body to remove the overproduced active oxygen and protect the living body, superoxide dismutase (hereinafter referred to as SOD) is generated inside the cell and balanced to ensure the health of the human body. ing.

然し乍ら、前記SODの活性は、壮年期から老年期になると急激に低下し、前記各種疾病が促進される。   However, the activity of the SOD rapidly decreases from the middle age to the old age, and the various diseases are promoted.

そこで、壮年期から老年期のSOD活性の低下を補助する為に、活性酸素消去能の優れた食品を提供しようとしてこの発明を完成したのである。   Therefore, in order to assist the decrease in SOD activity from the middle age to the old age, the present invention was completed in an attempt to provide a food having excellent active oxygen scavenging ability.

この発明によれば、米と大豆又は茶を含む食品の製造に際し、加熱温度と時間に留意したので、食品の風味その他を十分良好に保ちつつ活性酸素消去能の高い食品を提供することができる。   According to the present invention, since the heating temperature and time are taken into consideration in the production of foods containing rice and soybeans or tea, foods with high active oxygen scavenging ability can be provided while keeping the flavor and the like of the foods sufficiently good. .

また、前記食品を容易に製造できる効果がある。従って、癌その他の生活習慣病の予防に有効な食品を提供することができる。   Moreover, there exists an effect which can manufacture the said foodstuff easily. Therefore, a food effective for preventing cancer and other lifestyle-related diseases can be provided.

この発明によれば、活性酸素消去能を2〜5倍程度に高めた食品を製造することができる。そのため、がんや生活習慣病の予防および健康維持に有効な食品となることが期待される。また、活性酸素消去能は高められても、食品としての保存性、嗜好性などは低下することはないので従来製品と同様な取り扱い、摂取法が可能である。   According to this invention, the foodstuff which improved the active oxygen scavenging ability about 2 to 5 times can be manufactured. Therefore, it is expected to be an effective food for preventing cancer and lifestyle-related diseases and maintaining health. Moreover, even if the active oxygen scavenging ability is enhanced, the preservability, taste, etc. as a food are not lowered, and the same handling and intake methods as conventional products are possible.

この発明は、玄米と大豆粉又は豆乳を使用する食品又は玄米と大豆粉と緑茶を使用する食品或いは玄米と緑茶を含む食品などを製造する際の調理温度、加熱時間等を考慮して、活性酸素消去能の維持を図り、かつ従来品に優るとも劣らない食品を得るようにしたものである。   This invention is active in consideration of cooking temperature, heating time, etc. when producing food using brown rice and soy flour or soy milk, food using brown rice, soy flour and green tea or food containing brown rice and green tea, etc. The oxygen scavenging ability is maintained, and a food that is not inferior to conventional products is obtained.

前記における活性酸素消去能は、前記以外の材料(例えば、牛乳、砂糖その他の調味料)を加入しても変化はないので、食品材料について特別の制約はない。但し、玄米と大豆粉、豆乳、緑茶などの量が少ないと、活性酸素消去能についても減少するので、ある程度(例えば全量の10%以上)の量を保つことは必要である。また、相乗効果というからは、米と大豆、又は米と緑茶の混入割合は、等量を基準にして、20%〜50%(質量%)が多く用いられる。   The active oxygen scavenging ability in the above does not change even if other materials (for example, milk, sugar or other seasonings) are added, and there is no particular restriction on the food material. However, if the amount of brown rice, soy flour, soy milk, green tea, etc. is small, the active oxygen scavenging ability also decreases, so it is necessary to maintain a certain amount (for example, 10% or more of the total amount). Moreover, from a synergistic effect, the mixing ratio of rice and soybean or rice and green tea is often 20% to 50% (mass%) based on the equivalent amount.

この発明により製造される食品は、活性酸素消去物質を含有する農林水産原料とリン酸基を有する物質を含有する農林水産原料とを混合して製造される食品である。食品の形態、種類、摂取法に制限はない。活性酸素消去物質としては、ポリフェノールまたはフラボノイドのうちすくなくとも一種の物質が含有されていればよい。リン酸基を有する物質としてサイアミンまたはイノシトールのリン酸誘導のうち少なくとも、一種の物質を含んでいればよい。 The food produced according to the present invention is a food produced by mixing an agricultural, forestry and fishery raw material containing an active oxygen-eliminating substance and an agricultural, forestry and fishery raw material containing a substance having a phosphate group. There are no restrictions on the form, type, or intake of food. As the active oxygen scavenging substance, it is sufficient that at least one kind of polyphenol or flavonoid is contained. Of Thiamine or inositol phosphate induced body as a substance having a phosphate group at least may include at one substance.

加熱と混合が可能な容量2リットルのステンレス容器に牛乳400ml、大豆粉末100g、蒸留水300ml、豆乳400ml、玄米粉50g、トレハロース50gを順番に加えて混合し、60℃で徐々に加熱した。更に、寒天8gとバニラ1gを充分混合して、それらを更に加熱し、90℃に達してから30分間保持した。前記混合物を80℃に保温したまま、ポリエチレンカップに100mlずつ分注した。カップにPP/EVA/KNYのシートを被せ、カップにシートを熱シールし、大豆と米のプリンを生成した。   400 ml of milk, 100 g of soybean powder, 300 ml of distilled water, 400 ml of soy milk, 50 g of brown rice flour, and 50 g of trehalose were added in order to a stainless steel container having a capacity of 2 liters that can be heated and mixed, and heated gradually at 60 ° C. Further, 8 g of agar and 1 g of vanilla were mixed well, and they were further heated and held for 30 minutes after reaching 90 ° C. While keeping the mixture at 80 ° C., 100 ml each was dispensed into a polyethylene cup. The cup was covered with a PP / EVA / KNY sheet, and the cup was heat sealed to produce soybean and rice pudding.

活性酸素消去物質であるイソフラボンを100g中に280mg含有する大豆(品種名:リユウホウ)を水に一晩浸漬し、5倍量の水を加えて磨砕し、呉を得た。呉は102℃で5分間加熱したのちオカラを分離して豆乳を得た。この豆乳の活性酸素消去能を測定したところ、没食子酸に対する相対値(2mM没食子酸2200μlの発光強度を100とした場合の相対値)で40であった。サイアミンのリン酸誘導体を0.1mg/100g含む玄米(品種名:あきたこまち)を微粉砕し、その活性酸素消去能を測定したところ60であった。前記豆乳100gに対し、玄米粉10gを添加混合し、常法により豆腐を作製した。この豆腐は豆乳のみで製作した豆腐とほとんど味覚上の差はないが、活性酸素消去能は約3.5倍に増加していた。表1にそれぞれの原料および製品の活性酸素消去能を示す。

Figure 0004431783
Soybeans (variety name: Liuhou) containing 280 mg of isoflavone as an active oxygen scavenging substance in 100 g were soaked in water overnight and ground by adding 5 times the amount of water to obtain kure. After heating for 5 minutes at 102 ° C., Kure separated the okara to obtain soy milk. As a result of measuring the active oxygen scavenging ability of this soymilk, the relative value with respect to gallic acid (relative value when the emission intensity of 2200 μl of 2 mM gallic acid was taken as 100) was 40. Brown rice (variety name: Akitakomachi) containing 0.1 mg / 100 g of a phosphoric acid derivative of thiamine was finely pulverized, and its active oxygen scavenging ability was measured to be 60. To 100 g of the soymilk, 10 g of brown rice flour was added and mixed to prepare tofu by a conventional method. This tofu had almost no difference in taste from the tofu made with only soy milk, but the active oxygen scavenging ability increased about 3.5 times. Table 1 shows the active oxygen scavenging ability of each raw material and product.
Figure 0004431783

活性酸素消去能は、XYZ系活性酸素消去発光法により測定した。すなわち、196mM過酸化水素と炭酸水素カリ飽和の360mMアセトアルデヒドを試料に添加し、その際に生じる活性酸素消去発光を微弱発光測定装置(ARGAS−50 浜松ホトニクス)により計測した。活性酸素消去能は、代表的な消去物質である没食子酸2mM溶液200μlの発光強度を100とした場合の相対値で表した。   The active oxygen scavenging ability was measured by an XYZ-based active oxygen scavenging luminescence method. That is, 196 mM hydrogen peroxide and 360 mM acetaldehyde saturated with potassium hydrogen carbonate were added to the sample, and active oxygen elimination luminescence generated at that time was measured with a weak luminescence measuring apparatus (ARGAS-50 Hamamatsu Photonics). The active oxygen scavenging ability was expressed as a relative value when the luminous intensity of 200 μl of a 2 mM gallic acid solution, which is a typical scavenging substance, was taken as 100.

緑茶浸出液1000mlに対し、リン酸誘導体であるイノシトール6リン酸をソバ粉1gあたり6.5mg含むソバ粉100gを添加混合し、100℃で3分間加熱殺菌した。活性酸素消去能を測定した結果、表2の結果を得た。緑茶浸出液単独よりも活性酸素消去能の強い緑茶飲料が得られた。

Figure 0004431783
100 g of buckwheat flour containing 6.5 mg per 1 g of buckwheat flour, inositol 6-phosphate, which is a phosphoric acid derivative, was added to and mixed with 1000 ml of green tea leachate, and sterilized by heating at 100 ° C. for 3 minutes. As a result of measuring the active oxygen scavenging ability, the results shown in Table 2 were obtained. A green tea beverage with stronger active oxygen scavenging ability than green tea leachate alone was obtained.
Figure 0004431783

ジュール加熱可能で混合が可能な容量2リットルのステンレス容器に牛乳400ml、蒸留水400ml、玄米粉50g、トレハロース50gを順番に加えて混合し、60℃で徐々に加熱した。更に、寒天8gとバニラ1gを混合し10分間混合した。それらを更に加熱し、85℃に達してから30分間保持した。   In a 2 liter stainless steel container capable of joule heating and mixing, 400 ml of milk, 400 ml of distilled water, 50 g of brown rice flour, and 50 g of trehalose were added in order and mixed, and heated gradually at 60 ° C. Further, 8 g of agar and 1 g of vanilla were mixed and mixed for 10 minutes. They were further heated and held for 30 minutes after reaching 85 ° C.

ジュール加熱と混合が可能な容量2リットルのステンレス容器に牛乳400ml、豆乳400ml、玄米粉50g、トレハロース50gを順番に加えて混合し、60℃で徐々に加熱した。更に、寒天8gとバニラ1gを混合し10分間混合した。それらを更に加熱し、80℃に達してから40分間保持した。   400 ml of milk, 400 ml of soy milk, 50 g of brown rice flour and 50 g of trehalose were added in order to a 2 liter stainless steel container capable of Joule heating and mixing, and heated gradually at 60 ° C. Further, 8 g of agar and 1 g of vanilla were mixed and mixed for 10 minutes. They were further heated and held for 40 minutes after reaching 80 ° C.

[実験例]
加熱と混合が可能な容量2リットルのステンレス容器に牛乳400ml、豆乳400ml、玄米粉100g、蔗糖50gを順番に加えて混合し、徐々に60℃まで加熱し、その温度で10分間混合した。それらを100ml毎にビーカーに分注した後、70℃、80℃、90℃、100℃及び110℃で30分間加熱処理した。100℃の場合は沸騰水を、110℃の場合はオートクレーブを使用した。これらの温度処理物を室温まで冷却後に、活性酸素消去能を検定した。
[Experimental example]
In a stainless steel container having a capacity of 2 liters that can be heated and mixed, 400 ml of milk, 400 ml of soy milk, 100 g of brown rice flour, and 50 g of sucrose were sequentially added and mixed, gradually heated to 60 ° C., and mixed at that temperature for 10 minutes. They were dispensed every 100 ml into a beaker and then heat treated at 70 ° C., 80 ° C., 90 ° C., 100 ° C. and 110 ° C. for 30 minutes. Boiling water was used at 100 ° C., and an autoclave was used at 110 ° C. After these temperature-treated products were cooled to room temperature, the active oxygen scavenging ability was tested.

活性酸素消去活性は、XYZ系活性酸素消去発光法により測定した。即ち、活性酸素種(X)は196mM過酸化水素、メディエーター(Z)は炭酸水素カリウム飽和の360mMアセトアルデヒドである。微弱発光画像はARGAS50システム(浜松ホトニクス)により撮影し、その微弱発光強度により活性酸素消去活性を算出した。   The active oxygen scavenging activity was measured by an XYZ-based active oxygen scavenging luminescence method. That is, the reactive oxygen species (X) is 196 mM hydrogen peroxide, and the mediator (Z) is 360 mM acetaldehyde saturated with potassium hydrogen carbonate. The weak emission image was taken with the ARGAS 50 system (Hamamatsu Photonics), and the active oxygen scavenging activity was calculated from the weak emission intensity.

それらの結果を表3に示す。90℃までは活性酸素消去能が90%以上保存されたが、100℃、110℃では活性酸素消去能の著しい低下が認められた。

Figure 0004431783
The results are shown in Table 3. Up to 90 ° C., the active oxygen scavenging ability was preserved by 90% or more, but at 100 ° C. and 110 ° C., the active oxygen scavenging ability was significantly reduced.
Figure 0004431783

Claims (2)

玄米粉に対し、大豆粉、豆乳又は茶粉末の1種又は2種を20%〜50%(重量)容器に投入し、これに水を加え、又は水を加えることなくこれを撹拌混合して混合液を生成し、この混合液を60℃〜90℃の温度で30分〜50分間加熱することを特徴とした活性酸素消去能を有する米の加工食品の製造方法。Put one or two kinds of soybean powder, soy milk or tea powder into a 20% to 50% (weight) container with respect to the brown rice flour, add water to this, or stir and mix it without adding water. A method for producing a processed rice food having active oxygen scavenging ability, comprising producing a mixed solution and heating the mixed solution at a temperature of 60 ° C to 90 ° C for 30 minutes to 50 minutes. 請求項1記載の製造方法で製造したことを特徴とする活性酸素消去能を有する食品。A food having active oxygen scavenging ability, which is produced by the production method according to claim 1.
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