JP4426288B2 - 肝臓へのdnaのトランスフェクションの増強 - Google Patents
肝臓へのdnaのトランスフェクションの増強 Download PDFInfo
- Publication number
- JP4426288B2 JP4426288B2 JP2003512367A JP2003512367A JP4426288B2 JP 4426288 B2 JP4426288 B2 JP 4426288B2 JP 2003512367 A JP2003512367 A JP 2003512367A JP 2003512367 A JP2003512367 A JP 2003512367A JP 4426288 B2 JP4426288 B2 JP 4426288B2
- Authority
- JP
- Japan
- Prior art keywords
- liver
- dna
- ultrasound
- microspheres
- gas
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
- A61K48/0083—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the administration regime
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
- A61K48/0008—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'non-active' part of the composition delivered, e.g. wherein such 'non-active' part is not delivered simultaneously with the 'active' part of the composition
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
- A61K48/0075—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the delivery route, e.g. oral, subcutaneous
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/06—Antihyperlipidemics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Epidemiology (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Cardiology (AREA)
- Heart & Thoracic Surgery (AREA)
- Vascular Medicine (AREA)
- Diabetes (AREA)
- Hematology (AREA)
- Obesity (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicinal Preparation (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Description
したがって本発明の目的は、細胞、例えば哺乳動物の肝臓またはその他の器官における遺伝子発現を誘導する方法を提供することである。
を含む。
本発明は、ポリヌクレオチド、例えばDNAおよびRNAの哺乳動物の器官、例えば肝臓へのトランスフェクションのための材料および方法を含む。該方法は、高い注入圧、標的器官、例えば肝臓の超音波処置、または超音波処置および注入圧の組み合わせおよび/またはそれとマイクロバブルもしくはマイクロスフェアとの組み合わせ、またはこれらの手順のいずれかの組み合わせを用いてDNA含有溶液の脈管内投与を利用する。
Claims (7)
- アルブミンで安定化され気体を充填したマイクロスフェア及びプロモーターに作動可能に連結されているアポリポタンパク質A1(アポA1)をコードする裸のプラスミドDNAの混合物を含む組成物の、肝臓脈管構造内へ該組成物を注入すること、該組成物への暴露中に肝臓に超音波を適用し、肝臓におけるアポA1をコードするDNAのトランスフェクション及び発現を誘導することによる哺乳動物におけるアテローム性動脈硬化又は冠動脈心疾患の処置のための医薬の製造のための、使用。
- アルブミンで安定化され気体充填したマイクロスフェアが、ペルフルオロカーボンガスが充填されている、請求項1に記載の使用。
- アルブミンで安定化され気体充填したマイクロスフェアが、アルブミンで安定化され気体充填したマイクロスフェアである、請求項1に記載の使用。
- アルブミンで安定化され気体充填したマイクロスフェアが、ペルフルオロカーボンガス充填したマイクロスフェアである、請求項1に記載の使用。
- アルブミンで安定化され気体を充填したマイクロスフェア及びプロモーターに作動可能に連結されているアポリポタンパク質A1(アポA1)をコードする裸のプラスミドDNAの混合物を含む医薬組成物。
- アルブミンで安定化され気体充填したマイクロスフェアが、ペルフルオロカーボンガスが充填されている、請求項5に記載の組成物。
- アルブミンで安定化され気体充填したマイクロスフェアが、ペルフルオロカーボンガス充填したマイクロスフェアである、請求項5に記載の組成物。
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US30378401P | 2001-07-10 | 2001-07-10 | |
PCT/US2002/021620 WO2003006608A2 (en) | 2001-07-10 | 2002-07-10 | Enhancement of transfection of dna into the liver |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2005500327A JP2005500327A (ja) | 2005-01-06 |
JP4426288B2 true JP4426288B2 (ja) | 2010-03-03 |
Family
ID=23173680
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2003512367A Expired - Fee Related JP4426288B2 (ja) | 2001-07-10 | 2002-07-10 | 肝臓へのdnaのトランスフェクションの増強 |
Country Status (8)
Country | Link |
---|---|
US (2) | US7211248B2 (ja) |
EP (1) | EP1420643B1 (ja) |
JP (1) | JP4426288B2 (ja) |
AT (1) | ATE392812T1 (ja) |
AU (1) | AU2002320348B2 (ja) |
CA (1) | CA2452471C (ja) |
DE (1) | DE60226251T2 (ja) |
WO (1) | WO2003006608A2 (ja) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
TWI243696B (en) * | 2003-10-15 | 2005-11-21 | Der-Yang Tien | System for introducing macromolecule substance into living target cell |
US20110071381A1 (en) * | 2003-10-15 | 2011-03-24 | Robert Tien | Method and system for leading macromolecule substances into living target cells |
US8505544B2 (en) * | 2005-05-31 | 2013-08-13 | The Board Of Trustees Of The Leland Stanford Junior University | Optically-implemented microsurgery system and approach |
EP2123256A1 (en) | 2008-05-19 | 2009-11-25 | Ruhr-Universität Bochum | Perfluorcarbon nanoemulsions with endocytosis enhancing surface for gene-transfer |
US20110124716A1 (en) * | 2009-08-20 | 2011-05-26 | Allegheny-Singer Research Institute | Ultrasound-assisted gene transfer to salivary glands |
US9101745B2 (en) * | 2013-03-14 | 2015-08-11 | Sonogene Llc | Sonochemical induction of ABCA1 expression and compositions therefor |
US10052394B2 (en) | 2014-11-21 | 2018-08-21 | General Electric Company | Microbubble tether for diagnostic and therapeutic applications |
Family Cites Families (26)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB8721015D0 (en) * | 1987-09-07 | 1987-10-14 | Amersham Int Plc | Modifying living cells |
US5585112A (en) * | 1989-12-22 | 1996-12-17 | Imarx Pharmaceutical Corp. | Method of preparing gas and gaseous precursor-filled microspheres |
US5542935A (en) * | 1989-12-22 | 1996-08-06 | Imarx Pharmaceutical Corp. | Therapeutic delivery systems related applications |
US5580575A (en) * | 1989-12-22 | 1996-12-03 | Imarx Pharmaceutical Corp. | Therapeutic drug delivery systems |
JPH03297475A (ja) * | 1990-04-16 | 1991-12-27 | Ken Ishihara | 共振音波により薬物の放出を制御する方法 |
DE69215722T3 (de) * | 1991-03-22 | 2001-03-08 | Katsuro Tachibana | Verstärker zur Ultraschalltherapie von Erkrankungen sowie diesen enthaltende flüssige Arzneimittelzusammensetzungen |
CN1056124A (zh) | 1991-06-05 | 1991-11-13 | 清华大学 | 高效超声波基因导入方法 |
AU5457294A (en) * | 1992-10-29 | 1994-05-24 | Board Of Regents, The University Of Texas System | Adenovirus-mediated ldl receptor gene transfer and targeting |
EP0693924B2 (en) * | 1993-02-22 | 2008-04-09 | Abraxis BioScience, Inc. | Methods for (in vivo) delivery of biologics and compositions useful therefor |
HUT74509A (en) * | 1993-09-09 | 1997-01-28 | Schering Ag | Active principles and gas containing microparticles, their use for realising active principles in ultrasonically controlled manner, and process for preparing them |
US6048903A (en) * | 1994-05-03 | 2000-04-11 | Robert Toppo | Treatment for blood cholesterol with trans-resveratrol |
US5558092A (en) * | 1995-06-06 | 1996-09-24 | Imarx Pharmaceutical Corp. | Methods and apparatus for performing diagnostic and therapeutic ultrasound simultaneously |
US6139819A (en) * | 1995-06-07 | 2000-10-31 | Imarx Pharmaceutical Corp. | Targeted contrast agents for diagnostic and therapeutic use |
US6265387B1 (en) * | 1995-10-11 | 2001-07-24 | Mirus, Inc. | Process of delivering naked DNA into a hepatocyte via bile duct |
US20010009904A1 (en) * | 1997-12-30 | 2001-07-26 | Jon A. Wolff | Process of delivering a polynucleotide to a cell via the vascular system |
US5849727A (en) * | 1996-06-28 | 1998-12-15 | Board Of Regents Of The University Of Nebraska | Compositions and methods for altering the biodistribution of biological agents |
DE69738640T2 (de) | 1996-08-15 | 2009-07-16 | The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services | Räumliche und temporäre kontrolle der genexpression mittels eines hitzeschockproteinpromotors in kombination mit lokaler hitze |
WO1998017318A1 (en) * | 1996-10-19 | 1998-04-30 | Quadrant Healthcare (Uk) Limited | Use of hollow microcapsules in diagnosis and therapy |
US6120751A (en) * | 1997-03-21 | 2000-09-19 | Imarx Pharmaceutical Corp. | Charged lipids and uses for the same |
US6416740B1 (en) * | 1997-05-13 | 2002-07-09 | Bristol-Myers Squibb Medical Imaging, Inc. | Acoustically active drug delivery systems |
US6575956B1 (en) | 1997-12-31 | 2003-06-10 | Pharmasonics, Inc. | Methods and apparatus for uniform transcutaneous therapeutic ultrasound |
US6066123A (en) * | 1998-04-09 | 2000-05-23 | The Board Of Trustees Of The Leland Stanford Junior University | Enhancement of bioavailability by use of focused energy delivery to a target tissue |
US6135976A (en) * | 1998-09-25 | 2000-10-24 | Ekos Corporation | Method, device and kit for performing gene therapy |
WO2000042988A1 (en) | 1999-01-21 | 2000-07-27 | University Of Virginia | Method of identifying and treating inflamed tissue |
DE60026313D1 (de) * | 1999-07-23 | 2006-04-27 | Uutech Ltd | Sensibilisierung von roten blutkörperchen gegenüber ultraschall durch einwirkung eines elektrischen feldes |
IES990763A2 (en) * | 1999-09-09 | 2001-04-04 | Comtor Ltd | A Wrapping Method and Apparatus |
-
2002
- 2002-07-10 AT AT02749861T patent/ATE392812T1/de not_active IP Right Cessation
- 2002-07-10 DE DE60226251T patent/DE60226251T2/de not_active Expired - Lifetime
- 2002-07-10 CA CA2452471A patent/CA2452471C/en not_active Expired - Fee Related
- 2002-07-10 US US10/483,189 patent/US7211248B2/en not_active Expired - Lifetime
- 2002-07-10 JP JP2003512367A patent/JP4426288B2/ja not_active Expired - Fee Related
- 2002-07-10 AU AU2002320348A patent/AU2002320348B2/en not_active Ceased
- 2002-07-10 EP EP02749861A patent/EP1420643B1/en not_active Expired - Lifetime
- 2002-07-10 WO PCT/US2002/021620 patent/WO2003006608A2/en active IP Right Grant
-
2007
- 2007-04-16 US US11/787,289 patent/US20070196283A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
DE60226251T2 (de) | 2009-05-14 |
US7211248B2 (en) | 2007-05-01 |
AU2002320348B2 (en) | 2007-07-19 |
CA2452471A1 (en) | 2003-01-23 |
CA2452471C (en) | 2010-06-29 |
AU2002320348B8 (en) | 2003-01-29 |
EP1420643A2 (en) | 2004-05-26 |
US20040248832A1 (en) | 2004-12-09 |
EP1420643A4 (en) | 2005-01-05 |
ATE392812T1 (de) | 2008-05-15 |
EP1420643B1 (en) | 2008-04-23 |
WO2003006608A3 (en) | 2004-03-11 |
WO2003006608A2 (en) | 2003-01-23 |
US20070196283A1 (en) | 2007-08-23 |
JP2005500327A (ja) | 2005-01-06 |
DE60226251D1 (de) | 2008-06-05 |
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