JP4291884B2 - 新形成の治療用レオウイルス - Google Patents
新形成の治療用レオウイルス Download PDFInfo
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- JP4291884B2 JP4291884B2 JP51259099A JP51259099A JP4291884B2 JP 4291884 B2 JP4291884 B2 JP 4291884B2 JP 51259099 A JP51259099 A JP 51259099A JP 51259099 A JP51259099 A JP 51259099A JP 4291884 B2 JP4291884 B2 JP 4291884B2
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| WO2014204275A1 (ko) * | 2013-06-21 | 2014-12-24 | 한국생명공학연구원 | 리오바이러스를 유효성분으로 함유하는 혈관신생 억제용 조성물 |
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| US6565831B1 (en) | 1999-02-24 | 2003-05-20 | Oncolytics Biotech Inc. | Methods for preventing reovirus recognition for the treatment of cellular proliferative disorders |
| US6110461A (en) * | 1997-08-13 | 2000-08-29 | Oncolytics Biotech Inc. | Reovirus for the treatment of neoplasia |
| US7780962B2 (en) * | 1997-10-09 | 2010-08-24 | Wellstat Biologics Corporation | Treatment of neoplasms with RNA viruses |
| US7470426B1 (en) | 1997-10-09 | 2008-12-30 | Wellstat Biologics Corporation | Treatment of neoplasms with viruses |
| US20030044384A1 (en) * | 1997-10-09 | 2003-03-06 | Pro-Virus, Inc. | Treatment of neoplasms with viruses |
| CN1477964A (zh) * | 1999-04-15 | 2004-02-25 | 应用病毒治疗肿瘤 | |
| AU2005201079C1 (en) * | 1999-04-15 | 2008-11-06 | Wellstat Biologics Corporation | Treatment of neoplasms with viruses |
| US6586411B1 (en) | 2000-08-16 | 2003-07-01 | Mayo Foundation For Medical Education And Research | System for monitoring the location of transgenes |
| US8147822B1 (en) * | 1999-09-17 | 2012-04-03 | Wellstat Biologics Corporation | Oncolytic virus |
| US6896881B1 (en) * | 1999-09-24 | 2005-05-24 | Mayo Foundation For Medical Education And Research | Therapeutic methods and compositions using viruses of the recombinant paramyxoviridae family |
| CA2388807C (en) | 1999-11-12 | 2013-08-06 | Matthew C. Coffey | Viruses for the treatment of cellular proliferative disorders |
| AUPQ425699A0 (en) | 1999-11-25 | 1999-12-23 | University Of Newcastle Research Associates Limited, The | A method of treating a malignancy in a subject and a pharmaceutical composition for use in same |
| US7306902B2 (en) | 2002-06-28 | 2007-12-11 | Oncolyties Biotech Inc. | Oncolytic viruses as phenotyping agents for neoplasms |
| AR028039A1 (es) * | 2000-05-03 | 2003-04-23 | Oncolytics Biotech Inc | Remocion de reovirus de celulas neoplasticas intermediadas por ras a partir de composiciones celulares mixtas |
| US8491884B2 (en) | 2000-05-03 | 2013-07-23 | Oncolytics Biotech Inc. | Virus clearance of neoplastic cells from mixed cellular compositions |
| US20020147996A1 (en) * | 2000-07-07 | 2002-10-10 | Benjamin Thomas L. | Diagnosing and treating cancer cells using Sal2 |
| US20050013803A1 (en) * | 2000-07-07 | 2005-01-20 | Benjamin Thomas L. | Diagnosing and treating cancer cells using mutant viruses |
| US20020156039A1 (en) * | 2000-07-07 | 2002-10-24 | Benjamin Thomas L. | Diagnosing and treating cancer cells using Sal2 |
| US20030157481A1 (en) * | 2001-12-10 | 2003-08-21 | Benjamin Thomas L. | Diagnosing and treating cancer cells using T-HR mutants and their targets |
| TWI289158B (en) | 2000-08-10 | 2007-11-01 | Oncolytics Biotech Inc | Method of producing infectious reovirus |
| US7118740B1 (en) * | 2000-09-22 | 2006-10-10 | Mayo Foundation For Medical Education And Research | Method for limiting the growth of cancer cells using an attenuated measles virus |
| MXPA03004029A (es) | 2000-11-09 | 2004-02-12 | Oncolytics Biotech Inc | Metodos para tratamiento de trastornos proliferativos celulares. |
| JP2004513184A (ja) * | 2000-11-20 | 2004-04-30 | オンコリティクス バイオテック, インコーポレイティッド | 固形腫瘍塊への最適なウイルス送達法 |
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| AU2002220416A1 (en) * | 2000-12-01 | 2002-06-11 | University Of Ottawa | Oncolytic virus |
| AR035227A1 (es) | 2001-02-20 | 2004-05-05 | Oncolytics Biotech Inc | Uso de un agente quimioterapeutico para la manufactura de un medicamento para la sensibilizacion de celulas neoplasicas resistentes a agentes quimioterapeuticos con reovirus |
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| WO2003011230A2 (en) * | 2001-08-03 | 2003-02-13 | Board Of Regents, The University Of Texas System | Modified reoviral therapy |
| US20040115170A1 (en) * | 2001-11-30 | 2004-06-17 | Brown Earl Garnet | Oncolytic virus |
| CA2374388C (en) * | 2002-02-28 | 2005-07-12 | Matthew C. Coffey | The use of ribozymes in the detection of adventitious agents |
| US20040005546A1 (en) * | 2002-02-28 | 2004-01-08 | Oncolytics Biotech Inc. | Use of ribozymes in the detection of adventitious agents |
| US7319001B2 (en) | 2002-03-09 | 2008-01-15 | Neurogenex Co., Ltd. | High throughput system for producing recombinant viruses using site-specific recombination |
| JP2005523722A (ja) | 2002-04-30 | 2005-08-11 | オンコリティクス バイオテック, インコーポレイティッド | 改善されたウイルス精製方法 |
| EP1944035A1 (en) | 2002-05-09 | 2008-07-16 | Oncolytics Biotech Inc. | Method for reducing pain using oncolytic viruses |
| MXPA04011007A (es) * | 2002-05-09 | 2005-02-14 | Oncolytics Biotech Inc | Metodo para reducir el dolor usando virus oncoliticos. |
| AU2003238008A1 (en) * | 2002-06-12 | 2003-12-31 | Temple University Of The Commonwealth System Of Higher Education | Method of cell growth inhibition with agnoprotein |
| US7163678B2 (en) * | 2002-11-07 | 2007-01-16 | Oncolytics Biotech Inc. | Reovirus for the treatment of ral-mediated cellular proliferative disorders |
| AU2002953436A0 (en) * | 2002-12-18 | 2003-01-09 | The University Of Newcastle Research Associates Limited | A method of treating a malignancy in a subject via direct picornaviral-mediated oncolysis |
| US20060241178A1 (en) * | 2002-12-30 | 2006-10-26 | Immuneguard Llc | Compositions and methods for treating lung cancer |
| CN1788088B (zh) | 2003-03-27 | 2012-09-26 | 渥太华健康研究学会 | 突变的水泡性口炎病毒及其用途 |
| US7731974B2 (en) | 2003-03-27 | 2010-06-08 | Ottawa Hospital Research Institute | Mutant vesicular stomatitis viruses and uses thereof |
| WO2005002607A2 (en) * | 2003-07-07 | 2005-01-13 | Oncolytics Biotech Inc. | Oncolytic reoviruses for the treatment of neoplasms having activated pp2a or rac |
| WO2005014017A1 (ja) * | 2003-08-08 | 2005-02-17 | Yuji Shino | 癌治療用医薬組成物 |
| US7638318B2 (en) | 2003-09-26 | 2009-12-29 | Norvartis Ag | Seneca Valley virus based compositions and methods for treating disease |
| EP1668025B1 (en) * | 2003-09-26 | 2014-06-25 | Novartis AG | Seneca valley virus based compositions and methods for treating disease |
| US20050137152A1 (en) * | 2003-12-19 | 2005-06-23 | Danny Cheung | Reovirus for the prevention of neoplasia |
| WO2005111200A1 (en) * | 2004-05-17 | 2005-11-24 | Universite De Montreal | Novel strains of reoviruses and methods of uses thereof |
| US7901921B2 (en) | 2004-10-22 | 2011-03-08 | Oncolytics Biotech Inc. | Viral purification methods |
| US10668119B2 (en) | 2005-08-01 | 2020-06-02 | Virocure, Inc. | Attenuated reovirus |
| EP1917351A4 (en) * | 2005-08-01 | 2009-12-16 | Univ Technologies Int | ATTENUATED REOVIRUS |
| WO2007093036A1 (en) * | 2006-02-13 | 2007-08-23 | Oncolytics Biotech Inc. | Use of local immune suppression to enhance oncolytic viral therapy |
| CA2921063C (en) | 2006-09-15 | 2020-01-28 | Ottawa Hospital Research Institute | Oncolytic rhabdovirus |
| SG191602A1 (en) | 2007-03-12 | 2013-07-31 | Oncolytics Biotech Inc | Reoviruses having modified sequences |
| US10369171B2 (en) | 2007-03-13 | 2019-08-06 | Virocure, Inc. | Attenuated reoviruses for selection of cell populations |
| TW200909581A (en) * | 2007-05-21 | 2009-03-01 | Oncolytics Biotech Inc | Mutant reoviruses and methods of making and using |
| CA2723580A1 (en) * | 2008-05-27 | 2009-12-03 | Oncolytics Biotech Inc. | Modulating interstitial pressure and oncolytic viral delivery and distribution |
| EP2296679A4 (en) * | 2008-05-27 | 2012-03-21 | Oncolytics Biotech Inc | SUPPRESSION OF PRODUCTION OF PRO-INFLAMMATORY CYTOKINES DURING ONCOLYTIC REOVIRUS THERAPY |
| US9951117B2 (en) | 2010-09-02 | 2018-04-24 | Mayo Foundation For Medical Education And Research | Vesicular stomatitis viruses |
| JP5944901B2 (ja) | 2010-09-02 | 2016-07-05 | メイヨ・ファウンデーション・フォー・メディカル・エデュケーション・アンド・リサーチ | 水疱性口内炎ウイルス |
| WO2013163724A1 (en) * | 2012-04-30 | 2013-11-07 | Oncolytics Biotech Inc. | Protecting modified viruses from neutralizing antibodies using the reovirus sigma 1 protein |
| US20160271193A1 (en) | 2013-11-15 | 2016-09-22 | Oncolytics Biotech Inc. | Oncolytic viruses and increased cancer treatment regimens |
| KR20230012484A (ko) * | 2020-05-20 | 2023-01-26 | 바이로큐어 주식회사 | 암 예방 또는 치료용 약학적 조성물 |
Family Cites Families (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4108983A (en) * | 1976-06-01 | 1978-08-22 | The Wistar Institute | Viral oncolysate vaccine for stimulating the immune mechanism of mammals to species-specific tumors |
| SE445466B (sv) * | 1977-12-01 | 1986-06-23 | Wellcome Found | Sett att foroka rotavirus in vitro |
| NL7812359A (nl) * | 1978-12-20 | 1980-06-24 | Gist Brocades Nv | Vaccins tegen door reo virus veroorzaakte ziektever- schijnselen en werkwijze voor het bereiden van deze vaccins. |
| PT70556A (en) * | 1978-12-20 | 1980-01-01 | Gist Brocades Nv | Vaccines against diseases caused by reo-virus and process for the preparation of those vaccines |
| US4490358A (en) * | 1982-03-01 | 1984-12-25 | President And Fellows Of Harvard College | Screening vaccines and immunization process |
| US4559229A (en) * | 1982-07-20 | 1985-12-17 | Research Foundation | Avian proventriculitis vaccine |
| WO1990009441A1 (en) * | 1989-02-01 | 1990-08-23 | The General Hospital Corporation | Herpes simplex virus type i expression vector |
| WO1990011765A1 (en) * | 1989-04-11 | 1990-10-18 | Board Of Regents, The University Of Texas System | Antitumor preparation obtained following oncolysate treatment |
| CA2039921A1 (en) * | 1990-04-16 | 1991-10-17 | Xandra O. Breakefield | Transfer and expression of gene sequences into central nervous system cells using herpes simplex virus mutants with deletions in genes for viral replication |
| CA2051289C (en) * | 1990-09-14 | 2002-11-26 | Robert L. Martuza | Viral mediated destruction of neoplastic cells |
| DK0931830T3 (da) * | 1993-02-16 | 2001-06-11 | Onyx Pharma Inc | Cytopatiske vira til terapi og profylakse af neoplasi |
| US5525342A (en) * | 1994-05-20 | 1996-06-11 | Akzo Nobel, N.V. | Reovirus strain 2177 and vaccine containing same |
| US5853716A (en) * | 1995-07-28 | 1998-12-29 | Yale University | Genetically engineered chimeric viruses for the treatment of diseases associated with viral transactivators |
| TW349948B (en) | 1995-10-31 | 1999-01-11 | Janssen Pharmaceutica Nv | Farnesyl transferase inhibiting 2-quinolone derivatives |
| WO1998008541A1 (en) | 1996-08-30 | 1998-03-05 | Genzyme Corporation | Inhibition of primary and/or secondary immune response to repeat adenoviral vector administration using cd40l specific antibodies |
| US6136307A (en) * | 1997-08-13 | 2000-10-24 | Oncolytics Biotech Inc. | Reovirus for the treatment of cellular proliferative disorders |
| US6565831B1 (en) * | 1999-02-24 | 2003-05-20 | Oncolytics Biotech Inc. | Methods for preventing reovirus recognition for the treatment of cellular proliferative disorders |
| US6110461A (en) * | 1997-08-13 | 2000-08-29 | Oncolytics Biotech Inc. | Reovirus for the treatment of neoplasia |
| IL135507A0 (en) | 1997-10-09 | 2001-05-20 | Pro Virus Inc | Treatment of neoplasms with viruses |
| AR028039A1 (es) * | 2000-05-03 | 2003-04-23 | Oncolytics Biotech Inc | Remocion de reovirus de celulas neoplasticas intermediadas por ras a partir de composiciones celulares mixtas |
| MXPA03004029A (es) * | 2000-11-09 | 2004-02-12 | Oncolytics Biotech Inc | Metodos para tratamiento de trastornos proliferativos celulares. |
| AR035227A1 (es) * | 2001-02-20 | 2004-05-05 | Oncolytics Biotech Inc | Uso de un agente quimioterapeutico para la manufactura de un medicamento para la sensibilizacion de celulas neoplasicas resistentes a agentes quimioterapeuticos con reovirus |
| AU2003229180B8 (en) * | 2002-05-10 | 2009-07-02 | Oncolytics Biotech Inc. | Sensitization of neoplastic cells to radiation therapy with oncolytic viruses |
| US7163678B2 (en) * | 2002-11-07 | 2007-01-16 | Oncolytics Biotech Inc. | Reovirus for the treatment of ral-mediated cellular proliferative disorders |
| CA2621127C (en) * | 2005-08-31 | 2014-02-25 | Oncolytics Biotech Inc. | Treatment with an oncolytic virus and an immunostimulant for in vivo enhancement of immune system recognition of neoplasms |
| SG191602A1 (en) * | 2007-03-12 | 2013-07-31 | Oncolytics Biotech Inc | Reoviruses having modified sequences |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014204275A1 (ko) * | 2013-06-21 | 2014-12-24 | 한국생명공학연구원 | 리오바이러스를 유효성분으로 함유하는 혈관신생 억제용 조성물 |
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