JP4276539B2 - オメガ6pufaを含むコレステロール低下構造脂質 - Google Patents
オメガ6pufaを含むコレステロール低下構造脂質 Download PDFInfo
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- JP4276539B2 JP4276539B2 JP2003536362A JP2003536362A JP4276539B2 JP 4276539 B2 JP4276539 B2 JP 4276539B2 JP 2003536362 A JP2003536362 A JP 2003536362A JP 2003536362 A JP2003536362 A JP 2003536362A JP 4276539 B2 JP4276539 B2 JP 4276539B2
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- C11C3/04—Fats, oils, or fatty acids by chemical modification of fats, oils, or fatty acids obtained therefrom by esterification of fats or fatty oils
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
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- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Medicinal Chemistry (AREA)
- Hematology (AREA)
- Obesity (AREA)
- Diabetes (AREA)
- Fats And Perfumes (AREA)
- Edible Oils And Fats (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Medicines Containing Plant Substances (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Description
(a)加水分解およびエステル化
(b)エステル交換
(c)リパーゼ−エステル交換
(d)伝統的な化学的方法、または
(e)遺伝操作
によって生成される。
(a)植物油源のトリグリセリドを加水分解し、オメガ6 PUFAを含む遊離脂肪酸を得る工程と、
(b)ヤシ油を、前記(a)工程で得られた前記遊離脂肪酸で、モル比1:3で、エステル交換する工程と、
(c)酵素的酸分解のため、溶媒を用いて、37〜55℃の温度範囲で6〜48時間、固定化sn−1−3リパーゼとともに保温し、トリアシルグリセロールに、アシル基を導入する工程と、
(d)エーテル類から選択される溶媒およびヘキサンを用いた吸着クロマトグラフィーを用いて、反応生成物を分離し、構造脂質を得る工程とを含む。
(1)本発明は、天然源由来の脂肪酸を使用して、栄養および健康に対して潜在的な効果を有する新規の構造脂質を生成する。
(2)方法は、オメガ6脂肪酸を効果的に導入することによって、EFAが不足したヤシ油の改質のために使用することができた。
(3)生産は、方法の有効性を失うことなく、スケールアップすることができた。
(4)酸分解反応のためのトリグリセリドおよび脂肪酸の天然源の使用、および、活性損失なしに25サイクルまでの再利用が可能な固定化リパーゼの使用は、方法の経済的成功の可能性を保証する。
(5)栄養的に、構造脂質は、同様の脂肪酸組成を有する混合油や、改質されていないヤシ油よりも、コレステロール低下およびトリグリセリド低下において有効であることが判明した。
(6)MCFAおよびオメガ6PUFAに富んだ構造脂質は、本来、重症患者のための制限食に使用するための脂質源を提供し、これは、即座エネルギーを与えるラウリン酸を有し、オメガ6PUFA摂食でみられるエイコサノイド合成の変化は、損傷に対する炎症反応の低減および免疫適格を改善する。
(7)酵素的酸分解反応の結果得られる構造脂質は、ヤシ油や混合油と比較して、改善された溶融作用を示した。構造脂質は、改質されていないヤシ油よりも低い溶融温度を有し、そのため、低温でも液体状態を維持する。よって、構造脂質は、12〜15℃の温度で、相分離することなく、液体のままである。
Claims (11)
- ヤシ油を、紅花油のトリグリセリドの加水分解により得られた遊離脂肪酸と、エステル交換することによって得られ、46モル%のオメガ6多価不飽和脂肪酸と、17モル%のラウリン酸とを含む構造脂質。
- 請求項1に記載の構造脂質であって、前記構造脂質が、さらに、11モル%のミリスチン酸と、9モル%のパルミチン酸と、2モル%のステアリン酸と、15モル%のオレイン酸とを含む構造脂質。
- 相分離することなく液体として存在する、12〜15℃の非常に低い融点を有する、請求項1記載の構造脂質。
- 請求項1に記載のオメガ6多価不飽和脂肪酸(オメガ6 PUFA)を含む構造脂質の製造方法であって、
(a)植物油源のトリグリセリドを加水分解し、オメガ6 PUFAを含む遊離脂肪酸を得る工程と、
(b)ヤシ油を、前記(a)工程で得られた前記遊離脂肪酸で、モル比1:3で、エステル交換する工程と、
(c)酵素的酸分解のため、溶媒を用いて、37〜55℃の温度範囲で6〜48時間、固定化sn−1−3リパーゼとともに保温し、トリアシルグリセロールに、アシル基を導入する工程と、
(d)エーテル類から選択される溶媒およびヘキサンを用いた吸着クロマトグラフィーを用いて、反応生成物を分離し、構造脂質を得る工程とを含む方法。 - 脂肪酸が、紅花油の植物源から選択される請求項4記載の方法。
- エーテルが、石油エーテル、ジエチルエーテルから選択される請求項4記載の方法。
- 溶媒が、石油エーテル、ジオキサン、イソオクタン、n−ヘキサン、トルエンから選択される請求項4記載の方法。
- 使用されるエーテル:ヘキサンの比が、85:15〜95:5の範囲である請求項4記載の方法。
- エステル交換が、リパーゼ酵素を、基質の5〜10%(w/w)で用いて実施される請求項4記載の方法。
- 固定化リパーゼが、リゾムコール メイヘイ(Rhizomucor meihei)を用いて得られたものである請求項4記載の方法。
- リゾムコール メイヘイから得られた固定化リパーゼが、活性損失なしに、25サイクルまで使用可能である請求項4記載の方法。
Applications Claiming Priority (1)
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PCT/IN2001/000182 WO2003033632A1 (en) | 2001-10-18 | 2001-10-18 | Cholesterol lowering structured lipids with omega 6 pufa |
Publications (3)
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JP2005505682A JP2005505682A (ja) | 2005-02-24 |
JP2005505682A5 JP2005505682A5 (ja) | 2005-12-22 |
JP4276539B2 true JP4276539B2 (ja) | 2009-06-10 |
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JP2003536362A Expired - Fee Related JP4276539B2 (ja) | 2001-10-18 | 2001-10-18 | オメガ6pufaを含むコレステロール低下構造脂質 |
Country Status (5)
Country | Link |
---|---|
US (1) | US20030077340A1 (ja) |
EP (1) | EP1438378B1 (ja) |
JP (1) | JP4276539B2 (ja) |
AT (1) | ATE530626T1 (ja) |
WO (1) | WO2003033632A1 (ja) |
Families Citing this family (7)
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JP2007526943A (ja) | 2004-03-04 | 2007-09-20 | エイチティーエル ハイ − テック リピッズ リミテッド | 構造化されたトリグリセリド類およびそれを含むエマルション類 |
US20080261098A1 (en) * | 2007-04-20 | 2008-10-23 | General Electric Company | Proton-conducting membranes for electrochemical devices, and related articles and processes |
US7741576B2 (en) * | 2007-05-11 | 2010-06-22 | General Electric Company | Apparatus and method for hybrid machining a workpiece |
US7976694B2 (en) * | 2007-07-17 | 2011-07-12 | General Electric Company | Apparatus and method for hybrid machining a contoured, thin-walled workpiece |
US8183227B1 (en) | 2011-07-07 | 2012-05-22 | Chemo S. A. France | Compositions, kits and methods for nutrition supplementation |
US8168611B1 (en) | 2011-09-29 | 2012-05-01 | Chemo S.A. France | Compositions, kits and methods for nutrition supplementation |
GB201619827D0 (en) * | 2016-11-23 | 2017-01-04 | Lucite Int Uk Ltd | Process for the production of methyl methacrylate |
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US5190868A (en) * | 1987-08-31 | 1993-03-02 | Meito Sangyo Co., Ltd. | Continuous process for the interesterification of fats or oils |
DK565288D0 (da) | 1988-10-11 | 1988-10-11 | Novo Industri As | Fremgangsmaade til fremstilling af triglycerider, anvendelse af saadanne triglycerider og en emulsion, der indeholder saadanne triglycerider |
JP3102645B2 (ja) * | 1990-10-15 | 2000-10-23 | 雪印乳業株式会社 | 栄養補給用栄養組成物 |
CA2151820C (en) | 1993-01-15 | 2000-11-28 | Stephen Joseph Demichele | Structured lipids |
DK0893064T3 (da) | 1997-07-22 | 2003-04-22 | Nestle Sa | Lipidsammensætning til præparater til spædbørn samt fremstillingsfremgangsmåde for denne |
ATE306194T1 (de) * | 1999-02-12 | 2005-10-15 | Nestle Sa | Stabilisierte fettmischung, methode zu ihrer herstellung und lebensmittel, welches das fett enthält |
JP4152861B2 (ja) | 2003-10-27 | 2008-09-17 | シャープ株式会社 | コーナーキューブリフレクタ、その製造方法及びそれを用いた反射型表示装置 |
-
2001
- 2001-10-18 EP EP01980890A patent/EP1438378B1/en not_active Expired - Lifetime
- 2001-10-18 WO PCT/IN2001/000182 patent/WO2003033632A1/en active Search and Examination
- 2001-10-18 AT AT01980890T patent/ATE530626T1/de not_active IP Right Cessation
- 2001-10-18 JP JP2003536362A patent/JP4276539B2/ja not_active Expired - Fee Related
- 2001-12-10 US US10/014,842 patent/US20030077340A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
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WO2003033632A1 (en) | 2003-04-24 |
EP1438378A1 (en) | 2004-07-21 |
US20030077340A1 (en) | 2003-04-24 |
EP1438378B1 (en) | 2011-10-26 |
ATE530626T1 (de) | 2011-11-15 |
JP2005505682A (ja) | 2005-02-24 |
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