JP4249862B2 - Deodorants - Google Patents

Description

【００５２】
投与後14日間が経過した後、雌雄とも最大量の2000mg/kgでも、ラットの死亡は認められなかったので、致死量は2000mg/kgを上回るものと推定される。
飼育中いずれのラットにおいても異常は認められず、さらに各被検液投与群の雌雄の体重は、対照群とほぼ同等の推移を示し、観察期間中の体重増加も対照群とほぼ同等であった。また、いずれのラットにおいても、解剖学的検査の結果、体外表、頭部、胸部及び腹部の器官・組織に異常は見られなかった。
【００５３】
以上の結果から、製造例２で得られた甘蔗由来の蒸留物を使用し、ラットの単回経口投与毒性試験を行ったときの毒性は極めて弱いものと考えられる。
【００５４】
実施例１ 悪臭物質の化学消臭効果
１）アンモニアに対する消臭効果（検知管による測定）
▲１▼アンモニアガスの調製
500ml容三角フラスコに２８％アンモニア水（試薬）を0.5ml加え、迅速にパラフィルムで蓋をした。これを３０℃のインキュベーターに１０分間入れ、ガスを揮発させた。次に、注射器でフラスコ内のヘッドスペースのガスを10ml取り、３リットルの無臭空気の入った３リットル容の臭い袋に加えて一定濃度のアンモニアガスを得た。
▲２▼甘蔗由来の蒸留物の測定用サンプルおよび対照サンプルの調製
製造例２の甘蔗由来の蒸留物を、エタノール濃度が４５％（体積／体積）になるように蒸留水とエタノールを用いて３倍希釈した。次に、500ml容三角フラスコに四角く切った0.3ｇの脱脂綿を入れ、上記の希釈した蒸留物1mlを脱脂綿にできるだけ均一に吸わせ、ガラスコック付きシリコン栓で蓋をし、甘蔗由来の蒸留物の測定用サンプルとした。甘蔗由来の蒸留物を用いずに４５％（体積／体積）アルコールを用いて、対照サンプルを上記と同じ手順で用意した。
▲３▼測定
上記のアンモニアガスの入った３リットル容の臭い袋から３０mlのアンモニアガスをシリンジで取り、甘蔗由来の蒸留物の測定用サンプルおよび対照サンプルの入った三角フラスコにそれぞれ加え、さらにフラスコ内の圧力を陽圧にするため70mlの無臭空気をシリンジで加え、１０分後および３０分後のアンモニアガスの濃度を検知管で測定した。検知管で測定するには、サンプルを100ml吸引する必要があるため、フラスコ内を陽圧にしておく必要がある。なお、対照サンプルにおけるアンモニアの初期濃度をも測定して、この実験系のアンモニアガス初期濃度として結果の表に示す（以下、同様）。
２）トリメチルアミンに対する消臭効果（検知管による測定）
▲１▼トリメチルアミンガスの調製
３リットル容の臭い袋に90.0ppmのトリメチルアミン標準ガス（日本酸素株式会社製）を入れ、これを試験用ガスとした。
▲２▼甘蔗由来の蒸留物の測定用サンプルおよび対照サンプルの調製
アンモニアガスに対する消臭効果を測定する試験と同様に甘蔗由来の蒸留物の測定用サンプルおよび対照サンプルを調製した。
▲３▼測定
上記の３リットル容の臭い袋から100mlのトリメチルアミンガスをシリンジで取り、甘蔗由来の蒸留物の測定用サンプルおよび対照サンプルの入った三角フラスコにそれぞれ加え、１０分後および３０分後のトリメチルアミン濃度を検知管で測定した。
３）硫化水素に対する消臭効果（検知管による測定）
▲１▼硫化水素ガスの調製
試薬の硫化ナトリウム・９水和物22.0mgを100ml蒸留水に溶解し、20ml容ヘッドスペースバイアルに10ml入れ密栓した。このバイアルに、0.1Ｍ塩酸0.2mlを注射器でゆっくりと加え、その後３０℃で３０分間インキュベートした。インキュベート後のヘッドスペースガスを試験に用いる硫化水素ガスとした。
▲２▼甘蔗由来の蒸留物の測定用サンプルおよび対照サンプルの調製
アンモニアガスに対する消臭効果を測定する試験と同様に、甘蔗由来の蒸留物の測定用サンプルおよび対照サンプルを調製した。
▲３▼測定
上記のヘッドスペースバイアルのヘッドスペースガス2.0mlを注射器で取り、甘蔗由来の蒸留物の測定用サンプルおよび対照サンプルの入った三角フラスコに加え、さらにフラスコ内を陽圧にするため100mlの無臭空気を同様に加え、１０分後の硫化水素ガスの濃度を検知管で測定した。
４）イソ吉草酸に対する消臭効果（検知管による測定）
▲１▼イソ吉草酸ガスの調製
３リットルの無臭空気の入った３リットル容臭い袋に、試薬のイソ吉草酸（99％）0.1mlを注射器で注入し、迅速にパラフィルムで蓋をした。これを４０℃のインキュベーターに１０分間入れ、ガスを揮発させた。次に、フラスコ内のヘッドスペースのガス１リットルをシリンジで取り、２リットルの無臭空気の入った３リットル容臭い袋に加えて一定濃度のイソ吉草酸ガスを得た。
▲２▼甘蔗由来の蒸留物の測定用サンプルおよび対照サンプルの調製
アンモニアガスに対する消臭効果を測定する試験と同様に、甘蔗由来の蒸留物の測定用サンプルおよび対照サンプルを調製した。
▲３▼測定
上記のイソ吉草酸ガスの入った３リットル容の臭い袋から100mlのイソ吉草酸ガスをシリンジで取り、甘蔗由来の蒸留物の測定用サンプルおよび対照サンプルの入った三角フラスコにそれぞれ加え、１０分後のイソ吉草酸ガスの濃度を検知管で測定した。
５）メチルメルカプタンガスに対する消臭効果（検知管による測定）
▲１▼メチルメルカプタンガスの調製
３リットル容の臭い袋に93.7ppmのメチルメルカプタン標準ガス（日本酸素株式会社製）を入れた。次に、このガス300mlをシリンジで取り、３リットルの無臭空気の入った別の３リットル容の臭い袋に加え、一定濃度のメチルメルカプタンガスを得た。
▲２▼甘蔗由来の蒸留物の測定用サンプルおよび対照サンプルの調製
アンモニアガスに対する消臭効果を測定する試験と同様に、甘蔗由来の蒸留物の測定用サンプルおよび対照サンプルを調製した。
▲３▼測定
上記のメチルメルカプタンガスの入った３リットル容の臭い袋から、100mlのメチルメルカプタンガスをシリンジで取り、甘蔗由来の蒸留物の測定用サンプルおよび対照サンプルの入った三角フラスコに加え、１０分後のメチルメルカプタンの濃度を検知管で測定した。
６）硫化水素に対する消臭効果（ガスクロマトグラフィー分析）
▲１▼硫化水素ガスの調製
20ml容ヘッドスペースバイアルに360ｍＭ硫化ナトリウム溶液を0.1ml入れ、720ｍＭ塩酸0.1mlをゆっくりと加え混合した。これを３０℃のインキュベーターに３０分間入れ、ガスを揮発させた。このヘッドスペースガスを試験用硫化水素ガスとした。
▲２▼甘蔗由来の蒸留物の測定用サンプルおよび対照サンプルの調製
新たな20ml容ヘッドスペースバイアルに、アンモニアに対する消臭効果を測定したときと同様に希釈調製した製造例２の甘蔗由来の蒸留物１mlを入れ、密栓し、甘蔗由来の蒸留物の測定用サンプルとした。対照サンプルとして、甘蔗由来の蒸留物を含まない４５％（体積／体積）エタノール水溶液を用いた。
▲３▼測定
硫化水素ガスの入った▲１▼のバイアルから、ヘッドスペースガスをエアシリンジで0.04ml取り、▲２▼のバイアルに加え３０℃でインキュベートした。インキュベート開始から１５、３０、および４５分後にそのヘッドスペースガスを0.1ml採取し、ガスクロマトグラフィー分析を行った。
７）結果
消臭比は、次のようにして求めた。
消臭比＝[（Ｃ−Ｓ）／Ｃ]×１００
Ｃ：各時点における対照サンプル容器内の悪臭成分ガス濃度、Ｓ：各時点における甘蔗由来の蒸留物の測定用サンプル容器内の悪臭成分ガス濃度
結果を表２および表３に示した。甘蔗由来の蒸留物には悪臭物質の濃度を減少させる化学消臭効果があることが明らかになった。
悪臭物質として、尿、汗、生ゴミなどから発生する悪臭の原因物質のうち、代表的なものを選んで試験に用いたため、この結果は甘蔗由来の蒸留物に、広範囲の悪臭に対して高い除去効果があることを示している。
通常の消臭剤の消臭効果は、消臭剤と悪臭成分を混合してから６０分程経たないと現れないが、本実施例では甘蔗由来の蒸留物は１０〜１５分後には消臭効果を示しているので、即効性のある消臭剤であることが明らかになった。
【００５５】
【表２】

【００５６】
【表３】

【００５７】
実施例２ 布にしみ込ませた甘蔗由来の蒸留物の悪臭除去効果
製造例２で得られた甘蔗由来の蒸留物を、蒸留水で１０倍希釈した。これを木綿のサラシ（１０ｃｍ×１０ｃｍ）に0.4ｇスプレーし均一にしみ込ませた。この布を500ml容三角フラスコに入れ、ガラスコック付きシリコン栓で蓋をし、甘蔗由来の蒸留物の測定用サンプルとした。甘蔗由来の蒸留物を加えていない蒸留水を用いたものを用意し、対照サンプルとした。
悪臭成分として、酢酸、アセトアルデヒド、ホルムアルデヒド、トリメチルアミン、または硫化水素を含む試験用ガスを以下のようにして調製した。
酢酸1ml（99.5％試薬）を500ml容三角フラスコに入れ、迅速にパラフィルムで蓋をし、３０℃のインキュベーターに１０分間入れ、ガスを揮発させ、これを試験用酢酸ガスとした。
アセトアルデヒド（90％試薬）0.5mlを500ml容三角フラスコに入れ、迅速にパラフィルムで蓋をし、３０℃のインキュベーターに１０分間入れ、ガスを揮発させた。ここから10mlのガスをシリンジで取り、３リットルの無臭空気の入った臭い袋に注入し、臭い袋中のガスを試験用アセトアルデヒドガスとした。
ホルムアルデヒド（37％試薬）5mlを1000ml容三角フラスコに入れ、迅速にパラフィルムで蓋をし、３０℃のインキュベーターに１０分間入れ、ガスを揮発させた。ここから50mlのガスをシリンジで取り、３リットルの無臭空気の入った臭い袋に注入し、臭い袋中のガスを試験用ホルムアルデヒドガスとした。
トリメチルアミンの場合には、３リットル容の臭い袋に90.0ppmのトリメチルアミン標準ガス（日本酸素株式会社製）を入れ、これを試験用ガスとした。
また、硫化水素の試験用ガスは次のように調製した。試薬の硫化ナトリウム・９水和物22mｇを100mlの蒸留水に溶解し、これを20ml容ヘッドスペースバイアルに10ml入れ密栓した。この容器に、0.1Ｍ塩酸0.2mlを注射器でゆっくりと加え、その後３０℃で１０分間インキュベートした。インキュベート後のヘッドスペースガスを試験に用いる硫化水素ガスとした。
調製した試験用ガスは、酢酸の場合には10ml、アセトアルデヒド、ホルムアルデヒドおよびトリメチルアミンの３種の場合には100ml、硫化水素の場合には２mlをシリンジで取り、甘蔗由来の蒸留物の測定用サンプルの入ったフラスコに加えた。尚、フラスコ内を陽圧にするため酢酸の試験系には無臭空気90ml、硫化水素の試験系には無臭空気100mlを直ちに加えた。各場合において１時間後の悪臭成分の濃度を検知管で測定した。
消臭比は、次のようにして求めた：
消臭比＝[（Ｃ−Ｓ）／Ｃ]×１００
Ｃ：各時点における対照サンプル容器内の悪臭成分ガス濃度、Ｓ：各時点における甘蔗由来の蒸留物サンプル容器内の悪臭成分ガス濃度
結果を以下の表４に示した。甘蔗由来の蒸留物は、布にしみ込ませた場合にもその化学消臭効果が発揮されることが明らかになった。
この実験系では、汗などの代謝物や、建物の建材、壁紙、家具などに使用される化学物質から放出される悪臭の原因物質のうち、代表的なものを使用した。また、この実験系では甘蔗由来の蒸留物を布にしみ込ませて消臭効果を試験しているため、甘蔗由来の蒸留物がフィルター、カーテン、壁紙などに使用する消臭剤として有効であることが明らかになった。
【００５８】
【表４】

【００５９】
実施例３ 「一般消費者用芳香・消臭・脱臭剤の自主基準」に基づく効力試験法による評価
芳香消臭脱臭剤協議会による、「一般消費者用芳香・消臭・脱臭剤の自主基準」に基づく効力試験法による、液体消臭剤抗力試験を行った。
▲１▼0.05％アンモニア溶液の調製
２８％アンモニア水（試薬）に９５％（体積／体積）エタノールを加え、アンモニア濃度0.05％の溶液を調製した。
▲２▼甘蔗由来の蒸留物の測定用サンプルおよび対照サンプルの調製
１リットル容の三角フラスコに製造例２の甘蔗由来の蒸留物を１ｍｌ入れ、さらに上記で調製した0.05％アンモニア溶液0.05mlを入れてよく混ぜた後、ガラスコック付きシリコン栓で密栓し、甘蔗由来の蒸留物の測定用サンプルとした。また、別の１リットル容の三角フラスコには上記0.05％アンモニア溶液0.05mlのみを入れ、対照とした。
▲３▼測定
６０分後に検知管を用いて気相中の悪臭成分の濃度を測定し、対照の場合に対する蒸留物サンプルの場合の悪臭成分濃度の割合を、消臭比として求めた。
その結果、対照のアンモニア濃度は３８ppmであったのに対し、蒸留物サンプルのアンモニア濃度は７ppmであり、消臭比は８２％であった。
製造例２で得られた甘蔗由来の蒸留物は、気相中のアンモニアの濃度を明らかに抑えることができた。この実施例3から、甘蔗由来の蒸留物は、効果の高い消臭剤であると言える。
【００６０】
実施例４ メチルメルカプタンの官能試験による消臭効果の確認
製造例１で得られた甘蔗由来の蒸留物の原液、製造例２の蒸留物１００倍希釈液（希釈溶媒：水）、製造例３の蒸留物２０倍希釈液（希釈溶媒：水）を、1.5ml容エッペンドルフチューブに0.05ml入れた。これに1ppm（重量／体積）のメチルメルカプタン水溶液0.5mlを加え、良く攪拌したものを測定用サンプルとした。対照として、メチルメルカプタン溶液のみを入れたものを用いた。これらのサンプル及び対照を５人のパネラーに嗅がせ、消臭効果と不快度を評価した。
【００６１】
消臭効果はメチルメルカプタンのにおいを感じるかどうかを判断し、３：におわない、２：ほとんどにおわない、１：ややにおう、０：におう、の４段階で評価した。５人の評価の平均値を消臭効果として示した。
また、不快度はサンプルに含まれるにおい全体について、−４：極端に不快、−３：非常に不快、−２：不快、−１：やや不快、０：快でも不快でもない、の５段階で評価した。５人の評価の平均値を不快度として示した。
結果を以下の表５に示した。
対照はメチルメルカプタンそのものであり、はっきりとにおいが感じられ、極端に不快であるが、製造例１〜３で得られた甘蔗由来の蒸留物は、このにおいを明らかに抑えることができた。以上のことから、甘蔗由来の蒸留物は、効果の高い消臭剤であるといえる。
【００６２】
【表５】

【００６３】
実施例５ 食後臭の除去効果
５人のパネラーに、餃子定食（肉そばと餃子）を食べさせた後、歯を磨かせ、製造例１で得られた甘蔗由来の蒸留物を５０ｍｌ、製造例２の蒸留物を１００倍希釈（希釈溶媒：水）したものを５ｍｌ、または製造例３で得られた蒸留物を５０倍希釈（希釈溶媒：水）したものを５ｍｌ摂取させ、対照は未摂取とした。食後１時間目、２時間目の呼気を臭気測定用袋にサンプリングした。呼気のサンプルは、３７℃で５分間インキュベートした後、パネル３名による臭気官能試験法の９段階の快・不快度表示により評価した。
９段階の内容は、−４：極端に不快、−３：非常に不快、−２：不快、−１：やや不快、０：快でも不快でもない、＋１：やや快、＋２：快、＋３：非常に快、＋４：極端に快である。
官能試験の結果は、３名の平均値をとり以下の表６に示した。
この官能試験法では、１ポイントの差は、かなり明確なにおいの差であるため、甘蔗由来の蒸留物は高い食後臭除去効果を示すことが明らかになった。
【００６４】
【表６】

【００６５】
実施例６ 生ゴミ臭に対する消臭効果
生ゴミを捨てるための４５リットル容蓋付きプラスチックペールの内側に、スポンジ（10cm×10cm×2cm）８個をゴミに触れない位置につり下げ、１ヶ月間生ゴミを入れるために使用した。生ゴミは、３日ごとに違うものに取り替えた。１ヶ月後に、これらのスポンジを１つずつ蓋付きシャーレに入れ、このスポンジに製造例１で得られた甘蔗由来の蒸留物の原液、製造例２の蒸留物を１００倍希釈したもの、製造例３の蒸留物を５０倍希釈したもの、または対照としての蒸留水を、１０ｍｌスプレーし、蓋をした。５人のパネラーが、蒸留物スプレー直後と１時間後に、これらのスポンジのにおいを嗅ぎ、臭気官能試験法の９段階快・不快度表示により評価した。
９段階の内容は、−４：極端に不快、−３：非常に不快、−２：不快、−１：やや不快、０：快でも不快でもない、＋１：やや快、＋２：快、＋３：非常に快、＋４：極端に快である。
官能試験の結果は、５人の平均値をとり以下の表７に示した。
この官能試験法では、１ポイントの差はかなり明確なにおいの差であり、甘蔗由来の蒸留物には生ゴミ臭に対する高い消臭効果があることが明らかになった。
【００６６】
【表７】

【００６７】
実施例７ ミストスプレータイプ消臭剤
製造例１および２の消臭剤をそれぞれ２０％及び０．１％（共に体積／体積）になるように水で希釈し、ミストスプレーボトルに充填した。これらの試料を獣臭のある大型犬と、その犬小屋に噴霧したところ、犬も犬小屋も不快臭が消え、その効果は３日間持続した。
【００６８】
実施例８ エアゾールタイプ消臭剤
製造例１および２の消臭剤をそれぞれ６０％及び１％（共に体積／体積）となるように、またエタノール終濃度が２０％（体積／体積）になるようにエタノールおよび水で希釈し、得られた混合物の夫々を噴射ガス（ＬＰＧおよび二酸化炭素）と共にエアゾール容器に充填した。これらの試料を悪臭のある生ゴミに噴射したところ、悪臭が抑えられ、その効果は２４時間以上持続した。
【００６９】
実施例９ ゲル状消臭剤
カラギーナン２．２ｇを水８５ｇに添加し、良く攪拌しながら７０℃まで加熱し、完全に溶解させた。次にこの混合物を冷却して５０℃になったとき、製造例２で得られた消臭剤１５ｇを添加、混合し、全重量を１００ｇになるよう水で調整し、２００ｍｌ容量のビーカーに入れ、冷却・固化させ、ゲル状消臭剤試料を得た。このゲル状消臭剤を３個調製した。
この試料をそれぞれ２．６リットル容量のデシケーターに入れ、密閉し、２０℃で３時間安定化させた。悪臭物質であるトリメチルアミンガスを初期濃度約２０ｐｐｍになるようにこのデシケーターに注入し、２４時間後に悪臭濃度を検知管で測定し、悪臭減少率を求めた。測定が終了したゲル状消臭剤は次の測定までの間、３００mlビーカーに個別に入れ、２０℃、湿度６０％の恒温恒湿槽内で保存した。消臭効果の測定は、ゲル状消臭剤を調製した当日、調製７日後、調製１４日後に行い、悪臭物質の残存濃度から消臭率を求めた。結果を以下の表８に示した。なお、消臭率は、次のようにして求め、３個の消臭剤のデータを平均して示した：
［１−（測定終了時の悪臭物質濃度）／（測定開始時の悪臭物質濃度）］×１００
本実施例で調製したゲル状消臭剤は、効果の高い消臭活性を示した。
【００７０】
【表８】

【００７１】
実施例１０ 加湿器による室内消臭
悪臭のある老人ホームで特に悪臭の強い部屋を選び、試験対象とした。製造例２で得られた消臭剤を０．０５％（体積／体積）になるように水約１．５リットルに添加し、加湿器にセットした。加湿器の運転は間欠タイマーを用いて１５分間に１分間の割合で断続的に作動させた。その結果、一日中悪臭が抑えられた。
【００７２】
実施例１１ 畜舎（養豚場）消臭
製造例２と同様な条件で調製した消臭剤の０．１％（体積／体積）水溶液を調製し、これを消臭剤サンプルとした。これを養豚場の豚舎の加湿調整用の噴霧器のタンクに入れ、間欠タイマーで１５分間に１分間自動的に噴霧した。その結果、豚舎内の空気の不快度は明らかに改善した。
また、陽圧式ウインドレス型・スクレーパー除糞方式の豚舎の床面に消臭剤サンプルを床面１ｍ２（総床面積２５０ｍ２）当たり１５ｍｌ、１回散布した。散布前後の豚舎内の空気（床から１５ｃｍ上のところから採取）のメチルアミンとアンモニアの濃度を検知管で測定した。散布前の濃度に対する消臭率の経時変化を以下の表９に示した。
消臭剤の散布により、メチルアミンおよびアンモニアの濃度は明らかに減少した。
【００７３】
【表９】

【００７４】
実施例１２ 畜産糞尿
酪農農家で発生する牛の糞尿混合物を牧草地にバキュームカーを用いて散布し、散布直後に散布した周辺の５カ所の空気を、悪臭採取用の袋に無臭ポンプを用いて採取した。この空気をブランクとした。次に、散布した糞尿混合物をトラクターで土と混ぜた。
しばらくしてほとんどにおいが無くなった後、製造例２と同様な条件で調製した消臭剤０．６リットルを１００リットルの水に溶かし、バキュームタンクに入れ、ここに糞尿混合物６ｍ３を吸引し、これを牧草地に散布した。この散布直後に、上記と同様に散布した周辺の５カ所で空気を悪臭採取用の袋に無臭ポンプを用いて採取した。この空気をサンプルとした。
採取したブランクおよびサンプルを３リットル容量のにおい袋で１０倍に希釈し、パネル５名がにおいの評価を行った。評価は不快度で示し、−４：極端に不快、−３：非常に不快、−２：不快、−１：やや不快、０：快でも不快でもない、の５段階で評価した。各５地点、５人のパネルの不快度の平均は、ブランクが−３．２に対して、サンプルでは−１．８となり、明らかに不快度が低下した。
【００７５】
実施例１３ ペット用排泄物処理剤
製造例１および２で得られた消臭剤を用いて、ペット用排泄物処理剤を調製した。製造例１の消臭剤を用いた場合には、ベントナイト５０部、木粉５０部、消臭剤１０部、水４０部を用いた。製造例２でと同様な条件で調製した消臭剤を用いた場合には、ベントナイト５０部、木粉５０部、消臭剤１部、水５０部を用いた。原料はリボンミキサーで混合し、ディスク型のペレッターで直径３mm、長さ８〜２０mmのペレッターを得た。これをさらにロータリードライヤーで乾燥させたものを試料とした。また、対照として消臭剤を加えず、水を５０部用いたものを調製した。
これらの試料を２０ｇずつ５００ml容量の三角フラスコにとり、０．５％アンモニア水２mlを加え密栓し、３０℃、２０分間インキュベートした後、検知管で気体部分のアンモニア濃度を測定した。また、試料を入れずに同様の方法で試験し、測定されたアンモニア濃度をブランク値とし、消臭率を算出した。その結果を以下の表１０に示した。
対照にも含まれているベントナイトやゼオライトがアンモニアをある程度吸収するため、ブランクに比べ対照も消臭効果を有するが、本発明の消臭剤を使用した処理剤は明らかにそれ以上の消臭効果を示した。
【００７６】
【表１０】

【００７７】
【発明の効果】
本発明によれば、甘蔗由来の蒸留物を悪臭物質と接触させることにより、悪臭を低減もしくは消去することができる。しかも、甘蔗由来の蒸留物は植物由来であり、古来、ヒトがそのままかじっていた甘蔗、飲用にしていた甘蔗汁などの天然の飲食物中に存在している成分であるため、ヒト及び動物の健康を害することなく安全で、しかも環境にも安心して使用できる。また、本発明による消臭剤は、ほとんどにおいが無く、ほぼ無色透明であるため汎用性が高く、使用対象物のにおい、色に影響を与えない。また、環境消臭用に塗布、散布しても、環境を汚染することがない。これらのことから、産業上非常に有用である。[0001]
BACKGROUND OF THE INVENTION
The present invention relates to a deodorant containing a distillate derived from sweet potato as an active ingredient.
[0002]
[Prior art]
In recent years, with the improvement of living standards, consumers have come to emphasize not only the quantity and price of goods, but also the improvement and diversity of quality. The market area of deodorization and deodorization reflects these needs. In the food field, subtle differences in taste and fragrance, as well as various household goods including clothing, office supplies, furniture and even cars, in addition to their practicality and design diversity, antibacterial, insectproof, deodorant Additional functions such as deodorization have come to be regarded as important. In response, various deodorizing and deodorizing agents have been put on the market in recent years. For food applications, for human and animal body odors, for the environment, especially for individual malodorous objects, or for addition to foods and beverages, application and kneading to foods, spraying to the environment, sheets and grains There are various products depending on the type of deodorant / deodorant contained in the gel.
[0003]
Specifically, bad odors generated from living things such as humans and other animals, bad odors generated from indoors, cars, refrigerators, toilets, barns, fish tanks, factories, etc., household waste and industrial waste Deodorizing and deodorizing agents used to deodorize or deodorize bad odors are on the market.
[0004]
Of these deodorizing / deodorizing agents, those that are not intended to be directly ingested by humans or other animals, but are not intended to be in direct contact (deodorizing / deodorizing agents for environmental use) are based on mass production. Many chemical synthetic products are on sale. However, in consideration of the environment after use and disposal, it has been desired to use a natural deodorant / deodorant even for environmental use. In addition, safety is particularly required for deodorants that come into direct contact with humans and animals, and deodorants that are added to food and feed.
[0005]
Activated carbon is a natural deodorant that has been used for quite some time. Also, an environmental deodorant (Japanese Patent Laid-Open No. 62-2111068) in which activated carbon, an ion exchange resin, and a plant extract are mixed is known. Although these absorb and absorb odors, they need to be installed in the environment as a solid, and there is a problem that an installation place is required. Further, if the odor absorption is saturated after being left for a long time, the odor may be diffused from the substance that absorbs and adsorbs the odor.
[0006]
In addition, methods using various plant extracts have been developed as deodorizing / deodorizing agents derived from natural products. For example, one or two or more kinds of extracts of deodorizing / deodorizing agents (Japanese Patent Laid-Open No. 60-7848) extracted from an Oleaceae plant, Mitsumashi, Matsuri, Neubara, Primrose, Oleander, Tojitsu, and Denshichi A deodorant / deodorant containing a mixture (Japanese Patent Laid-Open No. 60-90563), a roasted extract of plant fiber material, and a concentrated solution obtained by concentrating after heating a raw material solution containing an acetate buffer, Deodorizing / preserving agent (Japanese Patent Laid-Open No. 60-227666), lettuce, cacao, coffee and parsley extract containing one or a mixture of two or more as active ingredients No. 60-207664), deodorant / deodorant containing an extract of camellia or camphoraceae as an active ingredient (Japanese Patent Laid-Open No. 61-106163), pine family plant How to obtain a deodorant component from the extract of (JP 63-21060 JP) and the like have been reported. In addition, a deodorizing sheet containing such a plant extract component (Japanese Patent Laid-Open No. 1-190443) has been reported.
[0007]
It has been clarified that the above plant extracts are effective components of deodorants, but most of them dry plants such as leaves, stems, and skins grown to obtain deodorants. Alternatively, after the roasting, it is obtained by crushing and extracting with an organic solvent or water, so that the production cost is high. In addition, depending on the plant and the extraction method, it contains plant-derived pigments and is colored even when diluted. Therefore, there is a problem that although it is desired to use only for the purpose of deodorization, it is colored by use. In addition, in the case of being colored and having a solid content, the colored evaporation residue may remain in the environment as a dry solid when applied or dispersed in the environment. Furthermore, although it has a deodorizing effect and can be used for environmental deodorization, there are those that cannot be ingested by humans and animals or whether it is safe when ingested.
[0008]
Next, as derived from sweet potato, brown sugar is said to have a deodorizing effect on fish odor and the like when cooking. However, since about 90% of brown sugar is sugar, and the strong smell of brown sugar itself, it has a deodorizing effect and hygiene. It is not possible to install it for deodorizing purposes. In addition, since it is necessary to use a large amount of brown sugar in order to exert a deodorizing effect even when used for food for the purpose of deodorization, sweetness is given and a strong smell of brown sugar itself is added. There was a problem that the application was limited.
[0009]
Further, a natural fragrance coconut flavor is known as an aroma component derived from brown sugar. Brown sugar is usually precipitated by adding lime milk to sweet potato press and agglomerating impurities, concentrating the sugar solution called purified juice to about Bx.60 with a concentrator such as a utility can, It is obtained by concentrating the concentrate to Bx.90 by heating to about 130 ° C. in an open pan and then cooling. In other words, the production of brown sugar requires the addition of lime milk and high-temperature heat concentration, especially due to chemical changes in the components that occur during the rapid high-temperature heat-concentration process at 130 ° C. An aroma component is generated. Numerous studies have been conducted on the aroma component of brown sugar, and it has been clarified that the flavor component peculiar to brown sugar is not contained in sweet squeezed juice (mixed juice) or purified juice (Food & Food Ingredients Journal No.156-1993, P58-65, New Flavor Vol.21 (8), P11-17 (1987)). Kokuto flavor, which is a natural fragrance produced as a fragrance for food additives, contains an aroma component unique to brown sugar. Usually, it is sold as a brown sugar flavor of a product by adding a fragrance of a chemically synthesized product to a natural coconut flavor. Such brown sugar flavors are a collection of fragrance components for flavoring purposes, so they have a strong fragrance and may have a deodorizing effect by masking unpleasant odors, but they are chemically and physically consumed. The deodorizing effect by the odor mechanism has not been clarified.
[0010]
The present applicant has reported a deodorizing substance derived from sweet potato in JP-A-10-151182. This deodorizing substance is a concentrate obtained by concentrating fractions obtained by treating sugar cane juice or molasses derived from sweet potato with column chromatography. This is a solvent distillation under reduced pressure by a distillation apparatus such as an evaporator. Alternatively, it can be concentrated and solidified by freeze drying or the like. It is usually concentrated liquid or powder, and the color is yellow to brown. The deodorant mechanism of this deodorant substance is not clear, but the main component of one or more types of deodorant substance is present in the colored dry solid, and this component neutralizes, adds, It is considered that the deodorizing effect is exerted by a complex reaction of various deodorizing mechanisms such as chemical deodorization such as oxidation and reduction, physical deodorization such as adsorption, sensory deodorization such as masking and counteracting action.
[0011]
[Problems to be solved by the invention]
The present invention is versatile, is derived from plants, has high safety, is inexpensive to produce, and does not remain as a solid in the environment to be deodorized. It is colorless or light-colored transparent, and does not itself have a strong odor. The object is to provide an odorant.
[0012]
[Means for Solving the Problems]
In view of the above problems, the present inventors have made extensive studies on a deodorant that is safe for animals such as humans and can be produced at low cost, and is itself colorless or light-colored and has almost no odor. However, the present invention was completed by finding that a distillate derived from sweet potato, which has been used as a food since ancient times, exhibits an excellent effect as a deodorant.
[0013]
That is, the present invention is a deodorant containing a distillate derived from sweet potato as an active ingredient.
[0014]
In one embodiment, the sweet potato-derived distillate is a distillate obtained by distilling a raw material selected from sweet potato juice and a sweet potato solvent extract.
[0015]
In a further embodiment, the sweet potato-derived distillate is obtained by treating a distillate obtained by distilling a raw material selected from sweet potato juice and a sweet potato solvent extract by column chromatography using a fixed carrier. It is a fraction.
[0016]
In another embodiment, the above operations are performed in the reverse order. First, a raw material selected from sweet potato soup and a sweet potato solvent extract is treated by column chromatography using a fixed carrier, and then the resulting fraction is distilled. The distillate thus obtained is used as an active ingredient of a deodorant.
[0017]
In the present invention, the column chromatography treatment is more preferably performed by using a column packed with a synthetic adsorbent as a fixed carrier, and the target sample is passed through the column, and the component adsorbed on the synthetic adsorbent is used. It is carried out by eluting with a solvent selected from water, methanol, ethanol and mixtures thereof.
[0018]
Here, the sweet potato juice in the present invention is a squeezed juice obtained by squeezing sweet potato (sugar cane), a brewed juice obtained by leaching sweet potato with water, or a lime-treated clean juice and concentrated juice in a raw sugar manufacturing factory. Includes. The solvent extract of sweet potato in the present invention means an extract obtained by extracting sweet potato with a general-purpose organic solvent. As the organic solvent immediately above, for example, methanol includes alcohols such as ethanol, and these may be used alone or in combination. Furthermore, these solvents and water may be used in combination.
[0019]
Specifically, the sweet potato-derived distillate of the present invention can be obtained by, for example, the following treatment.
[0020]
Ingredients selected from sweet potato juice and sweet potato solvent extract are heated by placing them in a tank with a heating device, and the resulting steam is recovered by cooling, or the steam is recovered as it is as a gas, derived from a liquid or gaseous sweet potato To obtain a distillate. For this distillation, any apparatus that has a heating device and can cool the vapor and recover it as a liquid or as it is as a gas can be used.
[0021]
Distillation conditions can be performed at a pressure of 50 to 120 ° C. at which the distillation raw material liquid boils. Preferably, it can be performed at a pressure of 70 to 120 ° C. at which the distillation raw material liquid boils. The pressure is appropriately adjusted according to the solvent used for extraction, the apparatus used in the distillation step, and the like. For example, when distilling using water as the extraction solvent and using a centrifugal thin film vacuum evaporator, a flask connected with a condenser tube, or a distiller, the pressure is 240 mmHg to normal pressure at 70 to 105 ° C. It is also possible to carry out distillation at lower temperatures and pressures. However, if it is desired to recover the vapor as a liquid, the lower the temperature, the higher the pressure must be reduced, and the distillate must be condensed below the distillation temperature in the trap. Too much. Therefore, although it is possible in the laboratory, it is not industrially suitable. In the distillation in the present invention, high temperature heating at 130 ° C. or higher is not performed. As a distillation apparatus, for example, a flask connected to a cooling pipe or the like is used in a laboratory, and a concentration can, a crystal can, a utility can or the like is used in a factory. By adjusting these temperature and pressure conditions, it is possible to obtain a distillate having a desired concentration by changing the ratio of components effective for deodorization and water (if water is contained) in the distillate. The obtained distillate can be used as an active ingredient of the deodorant of the present invention as it is as a gas or liquid, or diluted to an appropriate concentration with an appropriate solvent such as water.
[0022]
Moreover, a column chromatography process can be performed for the purpose of further concentrating the deodorizing active ingredient of the sweet potato-derived distillate obtained as described above. Alternatively, a raw material selected from the above-described sweet potato juice and sweet potato solvent extract is first subjected to column chromatography, and then a distillate can be obtained by the above method. Such a column chromatography method will be described below.
[0023]
First, a column chromatography method for recovering a sweet potato-derived distillate as a liquid and subjecting it to column chromatography, and a method for subjecting a raw material selected from sweet potato juice and a sweet potato solvent extract to column chromatography first will be described. .
[0024]
A raw material selected from the above-mentioned distillate (liquid) derived from sweet potato or a sweet potato juice and a sweet potato solvent extract may be passed through a column packed with a fixed carrier as it is or adjusted to an arbitrary concentration with water. it can. In addition, it is desirable to filter the raw material selected from the sweet potato soup and the sweet potato solvent extract before treating with a column in order to remove foreign substances. The filtration method is not particularly limited, and means such as screen filtration, diatomaceous earth filtration, microfiltration, and ultrafiltration that are widely used in the food industry can be preferably used.
[0025]
As the fixed carrier, a synthetic adsorbent is preferable. As the synthetic adsorbent, an organic resin can be preferably used. For example, an aromatic resin, an acrylic acid-based methacrylic resin, an acrylonitrile aliphatic resin, or the like can be used. Such synthetic adsorbents are commercially available, for example, HP-10, HP-20, HP-21, HP-30, HP-40, HP-50 (above, unsubstituted) as Diaion (trademark) series. Basic type aromatic resins, all trade names, manufactured by Mitsubishi Chemical Corporation); SP-825, SP-800, SP-850, SP-875, SP-70, SP-700 (above, unsubstituted group type) SPEC-900 (aromatic resin, trade name, manufactured by Mitsubishi Chemical Corporation); Amberlite (trademark), XAD -2, XAD-4, XAD-16, XAD-2000 (above, aromatic resins, all trade names, manufactured by Organo Corporation); Diaion (trademark) system, SP-205, SP-206, SP -207 (or more Aromatic resins having hydrophobic substituents, all trade names, manufactured by Mitsubishi Chemical Corporation; HP-2MG, EX-0021 (above, aromatic resins having hydrophobic substituents, both trade names, Mitsubishi Chemical Co., Ltd.); Amberlite (trademark) system, XAD-7HP, XAD-8 (above, acrylic ester resin, both trade names, manufactured by Organo Corporation); Diaion (trademark) system, HP1MG , HP2MG (above, acrylic acid-based methacrylic resin, both trade names, manufactured by Mitsubishi Chemical Corporation); Sephadex (trademark) as LH20, LH60 (above, cross-linked dextran derivatives, both trade names, Pharmacia Biotech Co., Ltd.) Manufactured). Since the type of the synthetic adsorbent suitable for the concentration of the liquid passing object, the solvent, the extraction method, the coexisting substances, and the like is different, the selection can be made as appropriate.
[0026]
The amount of the fixed carrier varies depending on the size of the column, the type of solvent, the type of fixed carrier, and the like. For example, when a sweet potato-derived distillate (liquid) is used, a distillate 1.5 to 20,000 times as large as the fixed carrier is passed through to adsorb the active ingredient to the fixed carrier. Therefore, it is preferable to use a fixed carrier having a wet volume of 1.5 to 20,000 times that of the distillate to be passed. Moreover, when using the raw material chosen from the sweet potato soup and the sweet potato solvent extract, it is preferable to use the fixed support | carrier of 0.01-5 times wet volume amount with respect to the solid content.
[0027]
By passing a liquid passing object (raw material selected from sweet potato-derived liquid distillate or sugar cane juice and sweet potato solvent extract) through the above column, the component having a deodorizing effect in the liquid passing object becomes a fixed carrier. Adsorbed. The flow rate and flow rate vary depending on the distillation method of the sweet potato-derived distillate or the raw material selected from the sweet potato juice and the sweet potato solvent extract. Further, when the liquid to be passed contains alcohol, it is necessary to dilute the liquid with water and then pass it through column chromatography. After passing the liquid passing object through the column, it is preferable to wash the column with water to remove impurities and remove components remaining in the column without being adsorbed by the resin.
[0028]
The component adsorbed on the fixed carrier is eluted with a solvent. The elution solvent is selected from water, methanol, ethanol and a mixture thereof. The elution solvent is preferably a mixed solvent of water and alcohol, particularly an ethanol-water mixed solvent. Furthermore, 50/50 to 99.5 / 0.5 (volume / volume) ethanol-water mixed solvent is preferable because a component having the desired effect can be efficiently eluted at room temperature. Thus, the component which has the effect of this invention exists in the fraction eluted with the said solvent. The elution rate is not particularly limited because it varies depending on the column size, the type of solvent, the type of fixed carrier, etc., but the eluate is eluted at SV = 0.1 to 10 and is eluted within 6 times the wet volume of the resin. Is preferably recovered. In addition, SV (Space Velocity, space velocity) is a unit of how many times the volume of the resin is passed per hour.
[0029]
The column chromatography treatment of the present invention is not limited to this, but can be preferably carried out as follows. That is, after passing an object to be passed through a column packed with an unsubstituted aromatic resin or acrylic ester resin at a column temperature of 60 to 97 ° C., the inside of the column is washed with water, and then placed in the column. The adsorbed components are eluted with 50/50 to 99.5 / 0.5 (volume / volume) ethanol-water mixed solvent at a column temperature of 20 to 40 ° C., and elution start time with the ethanol-water mixed solvent is started. The eluate that is eluted within 6 times the wet volume of the resin is collected.
[0030]
Thus, the eluate (including the elution solvent) obtained from the column can be used as it is as an active ingredient of the deodorant.
[0031]
Next, the case where the columnar chromatographic treatment of the sweet potato-derived distillate is described.
[0032]
A column filled with a porous adsorbent such as a zeolite adsorbent, activated carbon, carbon black, alumina, silica gel, a porous or fibrous adsorbent of a polymer adsorbent, or a ceramic porous adsorbent, and a vapor outlet of a distillation apparatus And pass the vapor through the column. The adsorbed components can be recovered by desorption by applying a temperature higher than that during distillation to the column or decreasing the internal pressure of the column. This temperature and pressure vary depending on the type of adsorbent selected. In this case, the component obtained by the column treatment comes out of the column as a gas, but can be cooled to a liquid by a trap. The column-treated liquid thus obtained can be used as it is as an active ingredient of a deodorant. In addition, components adsorbed on the column can be desorbed and recovered in the eluate by passing alcohols such as ethanol and methanol and other organic solvents through the column. The eluate (including the elution solvent) can be used as it is as an active ingredient of the deodorant.
[0033]
The distillate of the present invention has a slight odor in the stock solution, but has almost no odor when diluted when used. Therefore, although it is considered that there is no strong masking action, neutralization, decomposition, oxidation / reduction of chemical deodorization mechanism, adsorption of physical deodorization mechanism, offset action of sensory deodorization mechanism, and complex mechanism of masking action It is considered that one or more deodorizing main body substances having a deodorizing action due to the presence of the deodorizing substance exist in the distillate.
[0034]
The sweet potato-derived deodorant according to the present invention is mixed with other deodorants, fragrances, alcohol, surfactants, etc. as materials for foods, etiquette deodorants, pet deodorants, and environmental deodorants. Can be used. Specifically, the deodorant of the present invention can be added to foods to remove unpleasant odors of food materials such as meat, seafood, vegetables such as leek and garlic. Moreover, the deodorizer of this invention can be used as a raw material of an etiquette deodorizer. Here, the etiquette deodorant in the present invention is a deodorant used directly for humans such as bad breath, body odor such as armpit (for body), foot odor, hair odor and the like. This deodorant application means that it is used directly on humans regardless of the source of unpleasant odor. Specifically, underarm sweat odor sprays, lotions and powders, mouth odor removal mouse sprays, capsules, mouth washes, hair shampoos, rinses, hair lotions to remove the smell of roasted meat and cigarettes on the hair. Etc. The deodorant of the present invention can also be used as a material for pet deodorants. The pet deodorant of the present invention is a deodorant for unpleasant odors originating from pet animals such as halitosis, body odor, and fecal odor of pet animals. Specific examples include pet capsules for removing bad breath from pets, pet sprays, shampoos, rinses and lotions for removing body odor from hair, pet toilets, kennels and pet cages. Can be mentioned. Moreover, the deodorizer of this invention can be used also as a raw material of an environmental deodorizer. The environmental deodorant of the present invention is a deodorant for an unpleasant odor attached to an object or space other than a human or a pet animal. Specifically, disposal of household waste or industrial waste storage site, household waste or industrial waste collection site, household waste or industrial waste collection site, waste collection point, household waste or industrial waste disposal site, etc. Offensive odors from sources, sewage treatment plants, human waste treatment plants, crematoriums, slaughterhouses, slaughterhouses, hospitals / clinics / inspection centers, toilets, bathrooms, kitchens, etc., general indoor and indoor building materials And wallpaper (bad odors of processing agents used for processing such as formalin), curtains, painting, furniture (bad odors of processing agents such as paints and processing), mold odors such as indentations, shoe boxes, air conditioners, clothing, cars and trucks , Gas generated by cars and trucks, trains / aircraft, factories, restaurants, photo shops / developers, gas stations, propane gas refill stations, laundry shops / laundry factories, inns / hotels, beauty salons / science Shops, auto repair shop, livestock barns, can be used, such as the construction work site.
[0035]
Moreover, the usage form of the deodorizer of this invention is not specifically limited, For example, it can be used with the following forms. For spraying, it can be used as aerosol spray, mist spray, liquid product for sprinkler, liquid / gel / paste deodorant for application, and sheet form in which these deodorants are soaked in cloth / paper / nonwoven fabric. In addition, deodorants absorbed in powders and granules, deodorants kneaded and adsorbed into granular, pellet, block, and tablet gels, and adsorbed on porous carriers such as ceramics, activated carbon, and bentonite. If a deodorant or liquid deodorant is placed in a container and a liquid such as sponge, cloth or ceramics is partially in contact with the deodorant in the container, the permeated deodorant vaporizes. Deodorant with deodorant effect, deodorant put into a porous container such as ceramics, deodorant with deodorant effect by vaporizing the deodorant that penetrated to the outside of the container, liquid and bad odor as it is Deodorant added to the source, deodorant soaked in sheet film filter, wallpaper containing deodorant on the surface or inside, building materials, diapers, sanitary products, insole, deodorant fiber (cloth), Can be used for deodorant leather
[0036]
In addition, foods, etiquette deodorants, pet deodorants and environmental deodorants containing the deodorant of the present invention can contain additives, dispersants, excipients and the like commonly used in each field. .
[0037]
In order to prepare a mist spray type deodorant for household pet odor, toilet, kitchen garbage, cooking utensils, etc. using the deodorant of the present invention, more specifically, the following method is used. Can take. The deodorant of the present invention is added to water at a concentration of 0.01 to 50% (volume / volume), and if necessary, a surfactant, ethanol, antibacterial agent or the like is added and filled into a mist spray bottle. .
[0038]
More specifically, the following method can be used to prepare an aerosol-type deodorant for domestic garbage or a bathroom odor, which is a strong odor in the home, using the deodorant of the present invention. . The deodorant of the present invention is diluted with water or an aqueous ethanol solution to a concentration of 0.2 to 70% (volume / volume) and filled into an aerosol container together with a propellant gas (for example, LPG and carbon dioxide).
[0039]
In order to prepare a deodorant for a space where the deodorant active ingredient is gradually volatilized by adsorbing or kneading the deodorant of the present invention to a gel or a suitable carrier, and the deodorant effect is maintained for a long time. More specifically, the following method can be taken. Carrageenan, agar, locust bean gum, polyvinyl alcohol, gum arabic, gellan gum, gelatin, carboxymethylcellulose, chitin / chitosan, sodium alginate as a gelling agent at a concentration of 0.5 to 20% by weight of the deodorant of the present invention Add to a gel containing polyacrylamide alone or in combination and solidify.
[0040]
The deodorant of the present invention can be deodorized indoors by making it into a fine mist and scattering it in the room. More specifically, the deodorizer of the present invention is added to the water of the humidifier at a concentration of 0.01 to 1% (volume / volume), and the odor in the indoor space is deodorized by operating the humidifier. Can do.
[0041]
The deodorant of the present invention is intermittently or continuously sprayed in the air and sprayed on the floor surface to remove odors in places where odors are likely to occur such as pig farms, poultry farms, dairy livestock houses, fish markets, etc. can do. At this time, the deodorant of the present invention is preferably used after diluting with water to a concentration of 0.01 to 1% (volume / volume).
[0042]
In addition, the deodorant of the present invention can reduce bad odor when the feces, urine or manure mixture generated from pig farms, dairy farms, poultry farms, etc. is sprayed onto a field or pasture with a vacuum car or the like. . Odor can be suppressed by adding the deodorant of the present invention at a concentration of 0.005 to 0.5% (volume / volume) with respect to feces and urine in the tank before spraying.
[0043]
Moreover, the pet excrement disposal agent with a high deodorizing effect can be manufactured using the deodorizing agent of this invention. The excreta treating agent for pets is mainly composed of bentonite, zeolite, wood powder, paper powder, etc., and sodium polyacrylate, other sodium compounds, magnesium compounds, etc. are added to this as necessary. It can be produced by adding 0.01 to 10% by weight of a deodorant to the main material, adding an appropriate amount of water, mixing, molding and drying. By putting this in a pet toilet such as a cat, and the cat excreted on the treatment agent, a treatment agent having an excellent deodorizing effect can be obtained.
[0044]
In this way, in the method of mixing the other material and the deodorant of the present invention, molding and drying, the active ingredient of the deodorant remains without being completely evaporated during the drying, so the deodorizing effect also remains. . In addition, by mixing materials other than the deodorant of the present invention first, molding and drying, the article having the deodorizing effect can be obtained by absorbing the deodorant of the present invention in the obtained molded product. Can do.
[0045]
【Example】
In the following,% is% by weight unless otherwise specified. The concentration of gas in ppm is ppm (mol / mol) in accordance with the normal method for indicating the concentration of gas.
Production Example 1
Centrifugal thin-film vacuum evaporator (trade name: Evapol CEP-1, Okawara Seisakusho Co., Ltd.) using 2800 liters of sweet potato press (Bx.12.2) obtained in the raw sugar factory manufacturing process at a speed of 250 liters / hour The components distilled at a temperature of 90 to 95 ° C. under reduced pressure of 500 to 630 mmHg are cooled with a condenser under the conditions of a cooling water temperature of 25 ° C., a cooling water amount of 15 m 3 / hour, and a capacitor area of 2 m 2, continuously. collected. When the raw material press was about 2400 liters and Bx.13.9, distillation was completed. The obtained distillate was about 400 liters. Of this distillate, 500 ml was used as a deodorant sample which is a distillate derived from sweet potato. This sample was an almost colorless and transparent liquid.
[0046]
Production Example 2
About 400 liters of the distillate obtained in Production Example 1 was placed in a column (column size: inner diameter 2.6 cm, height 20 cm) packed with 40 ml of Amberlite XAD7HP (trade name, Organo Corporation) with SV = 75. The liquid was passed at a flow rate. After completion of the liquid flow, it was washed with water at the same flow rate for about 5 minutes. The adsorbed components were eluted with an 80% aqueous ethanol solution (ethanol / water = 80/20 (volume / volume)). The solution was passed at a flow rate of SV = 2, the first 25 ml of the eluate was discarded, and recovery of the eluate was started. After passing 80 ml of 80% ethanol aqueous solution, distilled water was passed at the same speed for extruding the components, and the elution was terminated when the amount of the recovered eluate reached 100 ml. The obtained eluate was used as a deodorant sample that is a distillate derived from sweet potato. As a result of measurement with an alcohol concentration meter (YSA-200, Yazaki Keiki Co., Ltd.), this sample was a slightly lemon-colored transparent liquid of ethanol 59% (volume / volume).
[0047]
Production Example 3
Squeeze sweet potato juice (Bx.12.5) obtained in the production process of the raw sugar manufacturing plant is heated to 80 ° C. with a juice heater and filtered with tube-type ultrafiltration (MH-25). A 750 liter treatment solution was obtained.
[0048]
A synthetic adsorbent SP-850 (trade name, Mitsubishi Chemical Co., Ltd.) 15 L was packed in a water jacketed column (column size: inner diameter 17.0 cm, height 100 cm), and the above-mentioned pressed juice filtration treatment liquid was added to the column. The liquid was passed at a flow rate of SV = 5. In addition, 65 degreeC water was always circulated through the water jacket during the passing of the pressed juice filtrate. After completion of the flow, washing was performed at the same flow rate until the eluate had a Brix of 0.1. Thereafter, a 55% ethanol aqueous solution (ethanol / water = 55/45 (volume / volume)) was passed at SV = 2 to elute the adsorbed components. During elution solvent flow, water at 25 ° C. was always circulated through the water jacket. The eluate for the first resin volume was discarded, and when elution of ethanol started, recovery of the eluate was started. After passing 30 liters of 55% ethanol aqueous solution, distilled water was passed at the same rate, and the eluate was collected until the ethanol concentration of the eluate was about 10%. The recovered eluate was brown and had an ethanol concentration of 37.5% (volume / volume) and about 45 liters. The ethanol concentration was measured with an alcohol concentration meter (YSA-200, Yazaki Keiki Co., Ltd.).
[0049]
The obtained eluate was distilled under normal pressure using a distiller, and the components distilled at a temperature of 70 to 100 ° C. were recovered by cooling with tap water around 20 ° C. Distillate was obtained. This distillate was used as a deodorant sample which is a distillate derived from sweet potato. This sample was a light yellow-green transparent liquid with an ethanol concentration of 48% (volume / volume).
[0050]
Test Example 1 Acute toxicity test of distillate derived from sweet potato
Using the sweet potato-derived distillate obtained in Production Example 2, a single oral dose toxicity test was conducted using rats. 16 male and female Sprague-Dawley SPF rats (Crj: CD (SD) IGS, Charles River Japan Co., Ltd.) were obtained at 5 weeks of age and quarantined and acclimatized for about 1 week. The test was conducted at 6 weeks of age. The body weight range at the time of administration was 174 to 186 g for males and 120 to 134 g for females.
As for the breeding conditions, the animal has a temperature of 23 ± 3 ° C., a relative humidity of 50 ± 20%, a ventilation rate of 1 to 10 to 15 times, and a solid feed (CRF-1 (trade name), oriental yeast in a breeding room with lighting for 12 hours a day. Co., Ltd.) and drinking water were bred freely.
Rats fasted overnight (about 16 hours) before administration were once orally gavaged with a predetermined concentration of sweet potato-derived distillate at a constant dose volume of 10 ml / kg body weight. A control group of animals was similarly administered only sterile distilled water. Refeeding after fasting was performed 6 hours after administration.
The dose was 1 dose of 2000 mg / kg, and a control group was added to this and a total of 2 groups were used. The number of animals in one group was 5 for both males and females.
The results are shown in Table 1 below.
[0051]
[Table 1]

[0052]
After 14 days from the administration, the mortality was estimated to exceed 2000 mg / kg because no death was observed in rats even at the maximum dose of 2000 mg / kg in both sexes.
No abnormalities were observed in any of the rats during breeding, and the body weights of both males and females in each test solution administration group were almost the same as those in the control group, and the weight gain during the observation period was almost the same as that in the control group. It was. In any rat, as a result of the anatomical examination, no abnormality was found in the organs / tissues of the external surface, head, chest and abdomen.
[0053]
From the above results, it is considered that toxicity is extremely weak when a single oral dose toxicity test is conducted on rats using the sweet potato-derived distillate obtained in Production Example 2.
[0054]
Example 1 Chemical deodorizing effect of malodorous substances
1) Deodorizing effect on ammonia (measurement with detector tube)
(1) Preparation of ammonia gas
0.5 ml of 28% aqueous ammonia (reagent) was added to a 500 ml Erlenmeyer flask and quickly capped with parafilm. This was placed in an incubator at 30 ° C. for 10 minutes to volatilize the gas. Next, 10 ml of the headspace gas in the flask was taken with a syringe and added to a 3 liter odor bag containing 3 liters of odorless air to obtain a constant concentration of ammonia gas.
(2) Preparation of sample for measurement of distillate derived from sweet potato and control sample
The distillate derived from sweet potato of Production Example 2 was diluted 3-fold with distilled water and ethanol so that the ethanol concentration was 45% (volume / volume). Next, 0.3 g of absorbent cotton cut into a square shape is placed in a 500 ml Erlenmeyer flask, and 1 ml of the diluted distillate is sucked into the absorbent cotton as evenly as possible, covered with a silicone stopper with a glass cock, A sample for measurement was used. A control sample was prepared in the same procedure as described above, using 45% (volume / volume) alcohol without a sweet potato-derived distillate.
(3) Measurement
Take 30 ml of ammonia gas from the 3 liter odor bag containing ammonia gas with a syringe and add it to the Erlenmeyer flask containing the sample for measurement of the distillate derived from sweet potato and the control sample. In order to obtain a positive pressure, 70 ml of odorless air was added with a syringe, and the concentration of ammonia gas after 10 minutes and 30 minutes was measured with a detector tube. In order to measure with a detection tube, it is necessary to suck 100 ml of the sample, so it is necessary to keep the inside of the flask at a positive pressure. The initial concentration of ammonia in the control sample was also measured and shown in the results table as the initial ammonia gas concentration in this experimental system (hereinafter the same).
2) Deodorizing effect on trimethylamine (measurement with detector tube)
(1) Preparation of trimethylamine gas
90.0 ppm of trimethylamine standard gas (manufactured by Nippon Oxygen Co., Ltd.) was placed in a 3-liter odor bag and used as a test gas.
(2) Preparation of sample for measurement of distillate derived from sweet potato and control sample
In the same manner as the test for measuring the deodorizing effect on ammonia gas, a sample for measurement of a distillate derived from sweet potato and a control sample were prepared.
(3) Measurement
Take 100 ml of trimethylamine gas from the above 3 liter odor bag with a syringe and add it to the Erlenmeyer flask containing the sugarcane-derived distillate measurement sample and the control sample. Measured with a detector tube.
3) Deodorizing effect on hydrogen sulfide (measurement with detector tube)
(1) Preparation of hydrogen sulfide gas
Reagent sodium sulfide 9hydrate 22.0 mg was dissolved in 100 ml distilled water, and 10 ml was put in a 20 ml headspace vial and sealed. To this vial, 0.2 ml of 0.1M hydrochloric acid was slowly added with a syringe and then incubated at 30 ° C. for 30 minutes. The head space gas after the incubation was used as the hydrogen sulfide gas used for the test.
(2) Preparation of sample for measurement of distillate derived from sweet potato and control sample
In the same manner as the test for measuring the deodorizing effect on ammonia gas, a sample for measurement of a distillate derived from sweet potato and a control sample were prepared.
(3) Measurement
Take 2.0 ml of headspace gas from the above headspace vial with a syringe and add it to the Erlenmeyer flask containing the measurement sample of the distillate derived from sweet potato and the control sample, and then add 100 ml of odorless air to make the inside of the flask positive. In addition, the concentration of hydrogen sulfide gas after 10 minutes was measured with a detector tube.
4) Deodorizing effect on isovaleric acid (measurement with detector tube)
(1) Preparation of isovaleric acid gas
0.1 ml of the reagent isovaleric acid (99%) was injected into a 3 liter odor bag containing 3 liters of odorless air with a syringe and quickly covered with parafilm. This was placed in an incubator at 40 ° C. for 10 minutes to volatilize the gas. Next, 1 liter of gas in the head space in the flask was taken with a syringe and added to a 3 liter odor bag containing 2 liters of odorless air to obtain a constant concentration of isovaleric acid gas.
(2) Preparation of sample for measurement of distillate derived from sweet potato and control sample
In the same manner as the test for measuring the deodorizing effect on ammonia gas, a sample for measurement of a distillate derived from sweet potato and a control sample were prepared.
(3) Measurement
Take 100 ml of isovaleric acid gas from the above 3 liter odor bag containing isovaleric acid gas with a syringe and add each to the Erlenmeyer flask containing the measurement sample of the sweet potato-derived distillate and the control sample for 10 minutes. The concentration of the subsequent isovaleric acid gas was measured with a detector tube.
5) Deodorization effect on methyl mercaptan gas (measurement by detector tube)
(1) Preparation of methyl mercaptan gas
93.7 ppm of methyl mercaptan standard gas (manufactured by Nippon Oxygen Co., Ltd.) was placed in a 3-liter odor bag. Next, 300 ml of this gas was taken with a syringe and added to another 3 liter odor bag containing 3 liters of odorless air to obtain a constant concentration of methyl mercaptan gas.
(2) Preparation of sample for measurement of distillate derived from sweet potato and control sample
In the same manner as the test for measuring the deodorizing effect on ammonia gas, a sample for measurement of a distillate derived from sweet potato and a control sample were prepared.
(3) Measurement
Take 100 ml of methyl mercaptan gas from the above 3 liter odor bag containing methyl mercaptan gas with a syringe and add it to the Erlenmeyer flask containing the sample for measuring distillate-derived distillate and the control sample. The concentration of methyl mercaptan was measured with a detector tube.
6) Deodorizing effect on hydrogen sulfide (gas chromatography analysis)
(1) Preparation of hydrogen sulfide gas
0.1 ml of 360 mM sodium sulfide solution was placed in a 20 ml headspace vial, and 0.1 ml of 720 mM hydrochloric acid was slowly added and mixed. This was placed in a 30 ° C. incubator for 30 minutes to volatilize the gas. This head space gas was used as a test hydrogen sulfide gas.
(2) Preparation of sample for measurement of distillate derived from sweet potato and control sample
In a new 20 ml headspace vial, put 1 ml of the sweet potato-derived distillate of Production Example 2 diluted and prepared in the same way as when measuring the deodorizing effect on ammonia, and tightly plug it. did. As a control sample, a 45% (volume / volume) ethanol aqueous solution not containing a sweet potato-derived distillate was used.
(3) Measurement
From the vial (1) containing hydrogen sulfide gas, 0.04 ml of headspace gas was taken with an air syringe, added to the vial (2), and incubated at 30 ° C. After 15, 30, and 45 minutes from the start of incubation, 0.1 ml of the headspace gas was sampled and analyzed by gas chromatography.
7) Results
The deodorization ratio was determined as follows.
Deodorization ratio = [(C−S) / C] × 100
C: Malodorous component gas concentration in the control sample container at each time point, S: Malodorous component gas concentration in the sample container for measurement of distillate derived from sweet potato at each time point
The results are shown in Tables 2 and 3. It was revealed that the sweet potato-derived distillate has a chemical deodorizing effect that reduces the concentration of malodorous substances.
As the malodorous substances generated from urine, sweat, garbage, etc., representative substances were selected for the test, so this result is high for a wide range of malodorous substances in sweet potato-derived distillates. It shows that there is a removal effect.
The deodorizing effect of a normal deodorant does not appear until about 60 minutes after mixing the deodorant and malodorous component, but in this example, the distillate derived from sweet potato is deodorized after 10 to 15 minutes. Since the effect was shown, it became clear that it was a deodorant with an immediate effect.
[0055]
[Table 2]

[0056]
[Table 3]

[0057]
Example 2 Deodorizing effect of distillate derived from sweet potato soaked in cloth
The sweet potato-derived distillate obtained in Production Example 2 was diluted 10 times with distilled water. 0.4 g of this was sprayed onto cotton sardine (10 cm × 10 cm) and uniformly impregnated. This cloth was placed in a 500 ml Erlenmeyer flask and capped with a silicon stopper with a glass cock to prepare a sample for measuring a sweet potato-derived distillate. A sample using distilled water not added with a sweet potato-derived distillate was prepared and used as a control sample.
Test gases containing acetic acid, acetaldehyde, formaldehyde, trimethylamine, or hydrogen sulfide as malodorous components were prepared as follows.
1 ml of acetic acid (99.5% reagent) was placed in a 500 ml Erlenmeyer flask, quickly capped with parafilm, placed in an incubator at 30 ° C. for 10 minutes to volatilize the gas, and this was used as test acetic acid gas.
0.5 ml of acetaldehyde (90% reagent) was placed in a 500 ml Erlenmeyer flask, quickly covered with parafilm, and placed in a 30 ° C. incubator for 10 minutes to volatilize the gas. 10 ml of gas was taken from this with a syringe and injected into an odor bag containing 3 liters of odorless air, and the gas in the odor bag was used as test acetaldehyde gas.
5 ml of formaldehyde (37% reagent) was placed in a 1000 ml Erlenmeyer flask, quickly covered with parafilm, and placed in a 30 ° C. incubator for 10 minutes to volatilize the gas. From this, 50 ml of gas was taken with a syringe and injected into an odor bag containing 3 liters of odorless air, and the gas in the odor bag was used as test formaldehyde gas.
In the case of trimethylamine, 90.0 ppm of trimethylamine standard gas (manufactured by Nippon Oxygen Co., Ltd.) was placed in a 3 liter odor bag and used as a test gas.
A hydrogen sulfide test gas was prepared as follows. The reagent sodium sulfide nonahydrate (22 mg) was dissolved in 100 ml of distilled water, and 10 ml of the solution was placed in a 20 ml headspace vial and sealed. To this container, 0.2 ml of 0.1M hydrochloric acid was slowly added with a syringe and then incubated at 30 ° C. for 10 minutes. The head space gas after the incubation was used as the hydrogen sulfide gas used for the test.
The prepared test gas is 10 ml for acetic acid, 100 ml for acetaldehyde, formaldehyde, and trimethylamine, and 2 ml for hydrogen sulfide with a syringe. Add to flask. In order to make the inside of the flask positive, 90 ml of odorless air was immediately added to the acetic acid test system and 100 ml of odorless air was immediately added to the hydrogen sulfide test system. In each case, the concentration of malodorous components after 1 hour was measured with a detector tube.
The deodorization ratio was determined as follows:
Deodorization ratio = [(C−S) / C] × 100
C: Malodorous component gas concentration in the control sample container at each time point, S: Malodorous component gas concentration in the sweet potato-derived distillate sample container at each time point
The results are shown in Table 4 below. It was revealed that the sweet potato-derived distillate exerts its chemical deodorizing effect even when it is soaked in cloth.
In this experimental system, representative substances were used among the causative substances of malodors emitted from metabolites such as sweat and chemical substances used for building materials, wallpaper, furniture, etc. In this experimental system, the sweet potato-derived distillate is soaked in a cloth and tested for its deodorizing effect. Became clear.
[0058]
[Table 4]

[0059]
Example 3 Evaluation by Efficacy Test Method Based on “Voluntary Criteria for General Consumer Aroma, Deodorant, and Deodorizer”
A liquid deodorant drag test was conducted by the Aroma Deodorant and Deodorant Council based on the efficacy test method based on the “Voluntary Standards for Fragrances, Deodorants and Deodorants for General Consumers”.
(1) Preparation of 0.05% ammonia solution
95% (volume / volume) ethanol was added to 28% aqueous ammonia (reagent) to prepare a solution having an ammonia concentration of 0.05%.
(2) Preparation of sample for measurement of distillate derived from sweet potato and control sample
Place 1 ml of the distillate derived from sweet potato of Production Example 2 in a 1 liter Erlenmeyer flask, add 0.05 ml of 0.05% ammonia solution prepared above and mix well, and then seal with a silicone stopper with a glass cock. A sample for measurement of the distillate was used. In another 1-liter Erlenmeyer flask, only 0.05 ml of the above 0.05% ammonia solution was placed as a control.
(3) Measurement
After 60 minutes, the concentration of the malodorous component in the gas phase was measured using a detector tube, and the ratio of the malodorous component concentration in the case of the distillate sample with respect to the control case was determined as the deodorization ratio.
As a result, the ammonia concentration of the control was 38 ppm, whereas the ammonia concentration of the distillate sample was 7 ppm, and the deodorization ratio was 82%.
The sweet potato-derived distillate obtained in Production Example 2 clearly reduced the concentration of ammonia in the gas phase. From Example 3, it can be said that the sweet potato-derived distillate is a highly effective deodorant.
[0060]
Example 4 Confirmation of deodorizing effect by sensory test of methyl mercaptan
The stock solution of the sweet potato-derived distillate obtained in Production Example 1, the distillate 100-fold dilution (dilution solvent: water) of Production Example 2, and the 20-fold dilution of the distillate of Production Example 3 (dilution solvent: water) 0.05 ml was put into a 1.5 ml Eppendorf tube. A sample for measurement was prepared by adding 0.5 ml of a 1 ppm (weight / volume) aqueous methyl mercaptan solution and stirring well. As a control, a solution containing only a methyl mercaptan solution was used. These samples and controls were sniffed by five panelists to evaluate the deodorizing effect and discomfort.
[0061]
The deodorizing effect was evaluated on the basis of whether or not the odor of methyl mercaptan was felt, and was evaluated in four stages: 3: no odor, 2: almost no odor, 1: slightly odor, 0: odor. The average value of five people's evaluation was shown as a deodorizing effect.
In addition, the discomfort level is divided into five levels: -4: extremely uncomfortable, -3: very uncomfortable, -2: uncomfortable, -1: slightly uncomfortable, 0: neither pleasant nor uncomfortable. evaluated. The average value of 5 people's evaluation was shown as discomfort.
The results are shown in Table 5 below.
The control was methyl mercaptan itself, which clearly had an odor and was extremely uncomfortable, but the sweet potato-derived distillate obtained in Production Examples 1 to 3 clearly suppressed this odor. From the above, it can be said that the sweet potato-derived distillate is a highly effective deodorant.
[0062]
[Table 5]

[0063]
Example 5 Removal effect of postprandial odor
Five panelists eat a dumpling set meal (meat soba and dumplings), then brush their teeth, dilute 50 ml of the sweet potato-derived distillate obtained in Production Example 1, and dilute the distillate of Production Example 2 100 times 5 ml of the diluted solvent (water) or 5 ml of the distillate obtained in Production Example 3 diluted 50 times (diluted solvent: water) was ingested, and the control was not ingested. 1 hour and 2 hours after the meal were sampled in an odor measurement bag. The sample of breath was incubated at 37 ° C. for 5 minutes, and then evaluated based on the nine levels of pleasantness / discomfort displayed by the panel odor sensory test method.
The contents of the 9 stages are: -4: extremely uncomfortable, -3: very uncomfortable, -2: uncomfortable, -1: slightly uncomfortable, 0: neither pleasant nor uncomfortable, +1: slightly pleasant, +2: pleasant, +3: Very pleasant, +4: Extremely pleasant.
The results of the sensory test are shown in Table 6 below, taking an average value of three people.
In this sensory test method, since the difference of 1 point is a fairly clear difference in odor, it became clear that the distillate derived from sweet potato shows a high postprandial odor removal effect.
[0064]
[Table 6]

[0065]
Example 6 Deodorizing effect on garbage odor
Eight sponges (10cm x 10cm x 2cm) were hung inside a plastic pail with a 45-liter lid for throwing away garbage, and used to put garbage for one month. The garbage was changed every 3 days. One month later, these sponges are placed one by one in a petri dish with a lid, and the stock solution of the sweet potato-derived distillate obtained in Production Example 1 and the distillate of Production Example 2 diluted 100-fold in this sponge, Production Example 10 ml of 3 distillates diluted 50-fold or distilled water as a control was sprayed and capped. Five panelists sniffed these sponges immediately after the distillate spray and 1 hour later, and evaluated them by a 9-step pleasant / discomfort degree display of the odor sensory test method.
The contents of the 9 stages are: -4: extremely uncomfortable, -3: very uncomfortable, -2: uncomfortable, -1: slightly uncomfortable, 0: neither pleasant nor uncomfortable, +1: slightly pleasant, +2: pleasant, +3: Very pleasant, +4: Extremely pleasant.
The sensory test results are shown in Table 7 below, taking an average value of 5 persons.
In this sensory test method, the difference of 1 point is a fairly clear odor difference, and it has been clarified that the sweet potato-derived distillate has a high deodorizing effect on the garbage odor.
[0066]
[Table 7]

[0067]
Example 7 Mist spray type deodorant
The deodorizers of Production Examples 1 and 2 were diluted with water to 20% and 0.1% (both volume / volume), respectively, and filled into mist spray bottles. When these samples were sprayed on a large dog with animal odor and its kennel, both the dog and the kennel disappeared and the effect persisted for 3 days.
[0068]
Example 8 Aerosol type deodorant
The deodorizers of Production Examples 1 and 2 were diluted with ethanol and water so that the deodorizers were 60% and 1% (both volume / volume) and the final ethanol concentration was 20% (volume / volume), respectively. Each of the resulting mixtures was filled into an aerosol container with a propellant gas (LPG and carbon dioxide). When these samples were sprayed on garbage with bad odor, the bad odor was suppressed and the effect lasted for more than 24 hours.
[0069]
Example 9 Gel-like deodorant
Carrageenan (2.2 g) was added to water (85 g), and the mixture was heated to 70 ° C. with good stirring to be completely dissolved. Next, when this mixture was cooled to 50 ° C., 15 g of the deodorant obtained in Production Example 2 was added and mixed, adjusted with water to a total weight of 100 g, and placed in a 200 ml capacity beaker. Then, it was cooled and solidified to obtain a gel-like deodorant sample. Three gel-like deodorants were prepared.
Each sample was placed in a 2.6 liter desiccator, sealed, and stabilized at 20 ° C. for 3 hours. Trimethylamine gas, which is a malodorous substance, was injected into this desiccator so as to have an initial concentration of about 20 ppm, and after 24 hours, the malodorous concentration was measured with a detector tube to determine the malodor reduction rate. The gel-type deodorant for which measurement was completed was individually placed in a 300 ml beaker until the next measurement, and stored in a constant temperature and humidity chamber at 20 ° C. and 60% humidity. The deodorizing effect was measured on the day of preparing the gel deodorant, 7 days after preparation, 14 days after preparation, and the deodorization rate was determined from the residual concentration of malodorous substances. The results are shown in Table 8 below. In addition, the deodorization rate was calculated | required as follows and the data of three deodorants were averaged and shown:
[1- (Odor substance concentration at the end of measurement) / (Odor substance concentration at the start of measurement)] × 100
The gel-like deodorant prepared in this example showed a highly effective deodorant activity.
[0070]
[Table 8]

[0071]
Example 10 Indoor deodorization with a humidifier
A room with a particularly strong odor in a nursing home with a bad odor was selected and used as a test subject. The deodorant obtained in Production Example 2 was added to about 1.5 liters of water so as to be 0.05% (volume / volume), and set in a humidifier. The operation of the humidifier was intermittently operated at a rate of 1 minute per 15 minutes using an intermittent timer. As a result, odors were suppressed throughout the day.
[0072]
Example 11 Slaughterhouse (pig farm) deodorization
A 0.1% (volume / volume) aqueous solution of a deodorant prepared under the same conditions as in Production Example 2 was prepared and used as a deodorant sample. This was put into a tank of a humidifier for adjusting humidity in a pig farm in a pig farm, and sprayed automatically for 1 minute every 15 minutes with an intermittent timer. As a result, the discomfort of the air in the pig house was clearly improved.
In addition, a deodorant sample was sprayed once at 15 ml per 1 m 2 floor (total floor area 250 m 2) on the floor surface of a positive pressure windless type scraper defecation piggery. The concentration of methylamine and ammonia in the pig house air before and after spraying (collected from 15 cm above the floor) was measured with a detector tube. Table 9 below shows the change over time in the deodorization rate with respect to the concentration before spraying.
The concentration of methylamine and ammonia was clearly reduced by the application of the deodorant.
[0073]
[Table 9]

[0074]
Example 12 Livestock Manure
Cattle manure generated from dairy farms was sprayed on pastures using a vacuum car, and the surrounding air sprayed immediately after spraying was collected using a odorless pump in a bag for collecting bad odors. This air was used as a blank. Next, the sprayed manure mixture was mixed with soil using a tractor.
After almost no smell after a while, 0.6 liters of deodorant prepared under the same conditions as in Production Example 2 was dissolved in 100 liters of water, placed in a vacuum tank, and 6 m3 of manure mixture was sucked into it. Was sprayed on the meadow. Immediately after this spraying, air was collected in a bad smell collecting bag using an odorless pump at five places around the sprayed area as described above. This air was used as a sample.
The collected blank and sample were diluted 10 times with a 3 liter odor bag, and five panelists evaluated the odor. The evaluation is indicated by the degree of discomfort, and was evaluated in five levels: -4: extremely uncomfortable, -3: very uncomfortable, -2: uncomfortable, -1: slightly uncomfortable, 0: neither pleasant nor uncomfortable. The average discomfort of the panels of 5 points and 5 persons was −1.8 for the sample compared to −3.2 for the blank, and the discomfort was clearly reduced.
[0075]
Example 13 Excrement Treatment Agent for Pet
Using the deodorant obtained in Production Examples 1 and 2, a pet excrement treating agent was prepared. When the deodorant of Production Example 1 was used, 50 parts of bentonite, 50 parts of wood flour, 10 parts of deodorant, and 40 parts of water were used. When a deodorant prepared under the same conditions as in Production Example 2 was used, 50 parts of bentonite, 50 parts of wood flour, 1 part of deodorant, and 50 parts of water were used. The raw materials were mixed with a ribbon mixer, and a pelleter having a diameter of 3 mm and a length of 8 to 20 mm was obtained with a disk-type pelleter. This was further dried with a rotary dryer as a sample. Moreover, what added 50 parts of water without adding a deodorizer as a control was prepared.
20 g of each sample was placed in a 500 ml Erlenmeyer flask, 2 ml of 0.5% aqueous ammonia was added and sealed, and incubated at 30 ° C. for 20 minutes. Then, the ammonia concentration in the gas portion was measured with a detector tube. Moreover, it tested by the same method without putting a sample, the ammonia concentration measured was made into the blank value, and the deodorizing rate was computed. The results are shown in Table 10 below.
Since the bentonite and zeolite contained in the control absorb ammonia to some extent, the control also has a deodorizing effect compared to the blank, but the treatment using the deodorant of the present invention clearly has a further deodorizing effect. showed that.
[0076]
[Table 10]

[0077]
【The invention's effect】
According to the present invention, the malodor can be reduced or eliminated by bringing the sweet potato-derived distillate into contact with the malodorous substance. Moreover, since the sweet potato-derived distillate is derived from plants, and since ancient times it is a component present in natural foods and drinks such as sweet potato that has been gnawed by humans as it is, It is safe without harming your health and can be used safely in the environment. Further, the deodorant according to the present invention has almost no odor and is almost colorless and transparent, so it is highly versatile and does not affect the odor or color of the object to be used. Moreover, even if it is applied and dispersed for environmental deodorization, it does not pollute the environment. From these things, it is very useful industrially.

Claims (9)

A deodorant comprising a sweet potato-derived distillate as an active ingredient, wherein a raw material selected from a sweet potato soup and a solvent extract of sweet potato is distilled at a pressure of the raw material boiling at 50 to 120 ° C. The distillate obtained in this way is passed through a column packed with a synthetic adsorbent as a fixed carrier, and the components adsorbed on the synthetic adsorbent are eluted with a solvent selected from water, methanol, ethanol and mixtures thereof. Deodorant characterized by being a fraction obtained by A deodorant comprising a sweet potato-derived distillate as an active ingredient, and a raw material selected from a sweet potato juice and a sweet potato solvent extract is passed through a column packed with a synthetic adsorbent as a fixed carrier, and the synthetic adsorption The component adsorbed on the agent is eluted with a solvent selected from water, methanol, ethanol and a mixture thereof, and the eluate obtained by the elution is obtained at a pressure of 50 to 120 ° C. at which the eluate boils. A deodorant characterized by being a fraction obtained by distillation. The deodorizer according to claim 1 or 2, wherein the synthetic adsorbent is an aromatic resin, an acrylic acid-based methacrylic resin, or an acrylonitrile aliphatic resin. The deodorizer according to any one of claims 1 to 3, wherein the elution is performed with a 50/50 to 99.5 / 0.5 (volume / volume) ethanol-water mixed solvent. The elution according to any one of claims 1 to 4, wherein the elution comprises recovering an eluate that elutes at a space velocity = 0.1 to 10 and elutes within 6 times the wet volume of the resin. Odorant. The foodstuff containing the deodorizer of any one of Claims 1-5 . An etiquette deodorant comprising the deodorant according to any one of claims 1 to 5 . The deodorizer for pets containing the deodorizer of any one of Claims 1-5 . The environmental deodorizer containing the deodorizer of any one of Claims 1-5 .