JP4095805B2 - ユビキチンリガーゼアッセイ - Google Patents
ユビキチンリガーゼアッセイ Download PDFInfo
- Publication number
- JP4095805B2 JP4095805B2 JP2001573019A JP2001573019A JP4095805B2 JP 4095805 B2 JP4095805 B2 JP 4095805B2 JP 2001573019 A JP2001573019 A JP 2001573019A JP 2001573019 A JP2001573019 A JP 2001573019A JP 4095805 B2 JP4095805 B2 JP 4095805B2
- Authority
- JP
- Japan
- Prior art keywords
- tag
- ubiquitin
- group
- curin
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/9015—Ligases (6)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/805—Optical property
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Hematology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Physics & Mathematics (AREA)
- Cell Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Genetics & Genomics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Feed For Specific Animals (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/542,497 US6740495B1 (en) | 2000-04-03 | 2000-04-03 | Ubiquitin ligase assay |
US09/542,497 | 2000-04-03 | ||
PCT/US2001/010906 WO2001075145A2 (en) | 2000-04-03 | 2001-04-03 | Ubiquitin ligase assay |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2003529372A JP2003529372A (ja) | 2003-10-07 |
JP4095805B2 true JP4095805B2 (ja) | 2008-06-04 |
Family
ID=24164065
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2001573019A Expired - Fee Related JP4095805B2 (ja) | 2000-04-03 | 2001-04-03 | ユビキチンリガーゼアッセイ |
Country Status (9)
Country | Link |
---|---|
US (5) | US6740495B1 (de) |
EP (1) | EP1268847B1 (de) |
JP (1) | JP4095805B2 (de) |
AT (1) | ATE377654T1 (de) |
AU (1) | AU2001251291A1 (de) |
DE (1) | DE60131262T2 (de) |
DK (1) | DK1268847T3 (de) |
ES (1) | ES2296744T3 (de) |
WO (1) | WO2001075145A2 (de) |
Families Citing this family (60)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7378248B2 (en) * | 2000-03-06 | 2008-05-27 | Rigel Pharmaceuticals, Inc. | In vivo production of cyclic peptides for inhibiting protein-protein interaction |
US6979551B2 (en) * | 2000-04-03 | 2005-12-27 | Rigel Pharmaceuticals, Inc. | Assays for identifying ubiquitin agents and for identifying agents that modify the activity of ubiquitin agents |
US6740495B1 (en) * | 2000-04-03 | 2004-05-25 | Rigel Pharmaceuticals, Inc. | Ubiquitin ligase assay |
US20020028472A1 (en) * | 2000-06-29 | 2002-03-07 | Michael Gmachl | Methods for identifying inhibitors of the anaphase promoting complex |
DE10108263A1 (de) * | 2001-02-21 | 2002-09-05 | Max Planck Gesellschaft | Analyse von Modifizierungen und Demodifizierungen von Proteinen mit Ubiquitinverwandten Proteinen mittels FRET (Fluorescence Resonance Energy Transfer) |
WO2003023405A1 (en) | 2001-09-07 | 2003-03-20 | Evotec Neurosciences Gmbh | Diagnostic and therapeutic use of f-box proteins for alzheimer's disease and related neurodegenerative disorders |
AU2002352797A1 (en) * | 2001-11-19 | 2003-06-10 | Proteologics, Inc. | Methods for identifying and validating potential drug targets |
US7736846B2 (en) * | 2002-08-30 | 2010-06-15 | Rigel Pharmaceuticals, Inc. | Methods of assaying for modulators of the inflammatory process using components of the ubiquitin ligation cascade |
US7723018B2 (en) | 2002-08-30 | 2010-05-25 | Rigel Pharmaceuticals, Incorporated | Methods of assaying for cell cycle modulators using components of the ubiquitin ligation cascade |
EP1867731A3 (de) | 2002-08-30 | 2008-03-12 | Rigel Pharmaceuticals, Inc. | Verfahren zum Testen von Modulatoren von Entzündungsprozessen über Teile der Ubiquitinbindungskaskade |
US7052843B2 (en) | 2002-10-08 | 2006-05-30 | New York University | Transcription-based assay for identification of post-translational modification and its application in proteomics |
US7312197B2 (en) * | 2003-02-24 | 2007-12-25 | University Of Maryland, Baltimore | Method of modifying glucose activity using polypeptides selectively expressed in fat tissue |
US20070122807A1 (en) * | 2003-04-03 | 2007-05-31 | Proteologics, Inc. | Posh polypeptides, complexes and related methods |
WO2005000876A2 (en) * | 2003-06-27 | 2005-01-06 | Proteologics, Inc. | Ring finger family proteins and uses related thereto |
US7132234B2 (en) * | 2003-10-28 | 2006-11-07 | Rigel Pharmaceuticals, Inc. | Methods and compositions for the identification of anti-poxvirus agents |
WO2005047476A2 (en) * | 2003-11-10 | 2005-05-26 | Rigel Pharmaceuticals, Inc. | Inhibition of retroviral replication through modulation of the host cell ubiquitylation |
EP1595945A1 (de) * | 2004-05-14 | 2005-11-16 | Boehringer Ingelheim International GmbH | Screeningverfahren zur Identifizierung von Verbindungen, die die Wirkung von Myc inhibieren können. |
JP2008517278A (ja) * | 2004-10-15 | 2008-05-22 | シグナル ファーマシューティカルズ,エルエルシー | p27のユビキチン化アッセイ及びその使用方法 |
US20090263821A1 (en) * | 2004-12-01 | 2009-10-22 | Proteologics, Inc. | Ubiquitin Ligase Assays And Related Reagents |
US9051572B2 (en) | 2005-01-20 | 2015-06-09 | Ryboquin Company Limited | Modulators of Itch ubiquitinase activity |
US20060246543A1 (en) * | 2005-03-03 | 2006-11-02 | President And Fellows Of Harvard College | Slim compositions and methods of use thereof |
US20070060537A1 (en) * | 2005-04-28 | 2007-03-15 | Proteologics, Inc. | Methods and compositions for inhibiting viral infections |
EP1891434B1 (de) * | 2005-06-02 | 2011-01-05 | The University of North Carolina at Chapel Hill | Reinigung, charakterisierung und wiederherstellung einer ubiquitin-e3-ligase |
KR100877824B1 (ko) * | 2005-11-11 | 2009-01-12 | 한국생명공학연구원 | E2epf ucp-vhl 상호작용 및 그 용도 |
US9066976B2 (en) | 2005-11-11 | 2015-06-30 | Korea Research Institute Of Bioscience And Biotechnology | Method for therapeutic angiogenesis |
US8058014B2 (en) * | 2006-09-29 | 2011-11-15 | University Of Maryland, Baltimore | Method of diagnosing or predicting disease states in a subject using omentin 1 and omentin 2 |
KR20090090383A (ko) | 2006-12-14 | 2009-08-25 | 다이이찌 산쿄 가부시키가이샤 | 이미다조티아졸 유도체 |
US20100099096A1 (en) * | 2007-02-28 | 2010-04-22 | The Brigham And Women's Hospital, Inc. | Compositions and Methods for Identifying Factors Affecting Protein Stability |
US7892772B2 (en) * | 2007-03-12 | 2011-02-22 | Iti Scotland Limited | Targeted ubiquitination of proteins and screening methods using a new class of ubiquitin ligase proteins |
MX344555B (es) | 2007-05-08 | 2016-12-20 | Hunter Douglas Ind Switzerland | Sistema de color multivariado con aplicacion de textura. |
AR069824A1 (es) | 2007-09-14 | 2010-02-24 | Basf Plant Science Gmbh | Plantas con rasgos aumentados relacionados con el rendimiento y un metodo para producirlas |
FR2924721B1 (fr) * | 2007-12-10 | 2010-02-26 | Cytomics Systems | Procede de criblage d'agents modulant l'activite de l'ubiquitine ligase mdm2 et moyens destines a la mise en oeuvre dudit procede |
WO2009134897A1 (en) * | 2008-04-29 | 2009-11-05 | Burnham Institute For Medical Research | E3-independent ubiquitinylation assay |
US8513181B2 (en) * | 2008-04-29 | 2013-08-20 | Cornell University | Substances and compositions for enhancing DNA repair and methods of use |
JPWO2009151069A1 (ja) | 2008-06-12 | 2011-11-17 | 第一三共株式会社 | 4,7−ジアザスピロ[2.5]オクタン環構造を有するイミダゾチアゾール誘導体 |
US20100021941A1 (en) * | 2008-07-25 | 2010-01-28 | Progenra, Incorporated | Methods of identifying modulators of ubiquitin ligases |
WO2010042828A2 (en) * | 2008-10-09 | 2010-04-15 | The Penn State Research Foundation | Methods and compositions relating to e3 ring-e2 fusion proteins |
PT2380892E (pt) | 2009-01-16 | 2014-06-09 | Daiichi Sankyo Co Ltd | Derivado de imidazotiazole compreendendo uma estrutura de anel prolina |
US20130131153A1 (en) * | 2009-11-16 | 2013-05-23 | The Johns Hopkins University | Cullin 5 as a regulator of hsp90 clients: a new target for drug development |
BR112012017165A2 (pt) | 2010-01-12 | 2015-09-15 | Ahram Biosystems Inc | aparelho de convecção térmica de três estágios e usos do mesmo. |
GB201006604D0 (en) | 2010-04-20 | 2010-06-02 | Iti Scotland Ltd | Ubiquitination assay |
GB201007185D0 (en) | 2010-04-29 | 2010-06-09 | Iti Scotland Ltd | Ubiquitination assay |
WO2011151826A2 (en) * | 2010-06-01 | 2011-12-08 | Ben-Gurion University Of The Negev Research And Development Authority | An expeditious synthesis of ubiquitinated peptide conjugates |
US9383367B1 (en) | 2010-12-07 | 2016-07-05 | Chunli Liu | Methods of detecting conjugation site-specific and hidden epitope/antigen |
WO2012121361A1 (ja) | 2011-03-10 | 2012-09-13 | 第一三共株式会社 | ジスピロピロリジン誘導体 |
WO2013042120A2 (en) | 2011-09-19 | 2013-03-28 | Proteologics Ltd | Synthetic ubiquitins and use thereof in drug screening assays |
CA2861066C (en) * | 2012-01-12 | 2024-01-02 | Yale University | Compounds and methods for the enhanced degradation of targeted proteins and other polypeptides by an e3 ubiquitin ligase |
EP2805281A4 (de) * | 2012-01-18 | 2015-09-09 | Singular Bio Inc | Verfahren zur abbildung von strichcodierten molekülen zur erkennung von strukturvariationen und sequenzierungen |
TWI586668B (zh) | 2012-09-06 | 2017-06-11 | 第一三共股份有限公司 | 二螺吡咯啶衍生物之結晶 |
GB201311888D0 (en) | 2013-07-03 | 2013-08-14 | Glaxosmithkline Ip Dev Ltd | Novel compounds |
GB201311891D0 (en) | 2013-07-03 | 2013-08-14 | Glaxosmithkline Ip Dev Ltd | Novel compound |
US20160282354A1 (en) | 2013-11-08 | 2016-09-29 | The Broad Institute, Inc. | Compositions and methods for selecting a treatment for b-cell neoplasias |
US10071164B2 (en) | 2014-08-11 | 2018-09-11 | Yale University | Estrogen-related receptor alpha based protac compounds and associated methods of use |
WO2016138574A1 (en) | 2015-03-02 | 2016-09-09 | Sinai Health System | Homologous recombination factors |
WO2017044793A1 (en) | 2015-09-11 | 2017-03-16 | The Brigham And Women's Hospital, Inc. | Methods of characterizing resistance to modulators of cereblon |
CN108366992A (zh) | 2015-11-02 | 2018-08-03 | 耶鲁大学 | 蛋白水解靶向嵌合体化合物及其制备和应用方法 |
US11604186B2 (en) * | 2018-10-17 | 2023-03-14 | Molecular Devices (Austria) GmbH | Real time western blot assays utilizing fluorescence resonance energy transfer (FRET) |
CN111208243B (zh) * | 2018-11-21 | 2022-05-17 | 中国科学院大连化学物理研究所 | 一种基于阴离子交换色谱柱的sumo化肽段的富集方法 |
WO2022187342A1 (en) * | 2021-03-02 | 2022-09-09 | The Board Of Regents Of The University Of Texas System | Methods and compositions for detecting protein targets |
WO2023096721A1 (en) * | 2021-11-23 | 2023-06-01 | Board Of Regents, The University Of Texas System | Fret-active e2-ubl conjugates and uses thereof |
Family Cites Families (38)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5997856A (en) * | 1988-10-05 | 1999-12-07 | Chiron Corporation | Method and compositions for solubilization and stabilization of polypeptides, especially proteins |
GB9214857D0 (en) | 1992-07-13 | 1992-08-26 | Medical Res Council | Human nucleic acid fragments and their use |
US5384255A (en) * | 1993-06-21 | 1995-01-24 | Rappaport Family Institute For Research In The Medical Sciences | Ubiquitin carrier enzyme E2-F1, purification, production, and use |
DE69517010T2 (de) * | 1994-01-04 | 2000-12-21 | Mitotix Inc | Ubiquitin-konjugierende enzyme |
US5744343A (en) * | 1994-01-04 | 1998-04-28 | Mitotix, Inc. | Ubiquitin conjugating enzymes |
WO1995027066A2 (en) * | 1994-03-31 | 1995-10-12 | Univ Leeds | Dna encoding ubiquitin conjugating enzymes |
US6127158A (en) * | 1994-12-07 | 2000-10-03 | President And Fellows Of Harvard College | Ubiquitin conjugating enzymes |
US5726025A (en) * | 1995-04-20 | 1998-03-10 | President And Fellows Of Harvard College | Assay and reagents for detecting inhibitors of ubiquitin-dependent degradation of cell cycle regulatory proteins |
US6001619A (en) * | 1995-10-04 | 1999-12-14 | Cold Spring Harbor Laboratory | Ubiquitin ligases, and uses related thereto |
US5861249A (en) * | 1996-04-23 | 1999-01-19 | Cold Spring Harbor Laboratory | Assays and reagents for identifying modulators of cdc25-mediated mitotic activation |
US5840866A (en) * | 1996-07-10 | 1998-11-24 | Incyte Pharmaceuticals, Inc. | Human ubiquitin-conjugating enzyme |
EP0843008A1 (de) | 1996-11-14 | 1998-05-20 | BOEHRINGER INGELHEIM INTERNATIONAL GmbH | Verfahren zur Herstellung von dem Anaphase-fördernden Komplex |
US5817494A (en) * | 1997-05-21 | 1998-10-06 | Incyte Pharmaceuticals, Inc. | Ubiquitin conjugation proteins |
US6060262A (en) * | 1997-07-16 | 2000-05-09 | Mitotix, Inc. | Regulation of I Kappa B (IκB) degradation and methods and reagents related thereto |
AU8555798A (en) | 1997-08-01 | 1999-02-22 | Genset | 5' ests for secreted proteins expressed in muscle and other mesodermal tissues |
EP1000151B1 (de) | 1997-08-01 | 2006-05-31 | Serono Genetics Institute S.A. | 5' ests für sekretierte proteine die in verschiedenen geweben expremiert werden |
AU9664598A (en) * | 1997-09-23 | 1999-04-12 | Incyte Pharmaceuticals, Inc. | Human ubiquitin-conjugating enzymes |
US6573094B1 (en) * | 1997-10-16 | 2003-06-03 | Baylor College Of Medicine | F-box genes and proteins |
US6426205B1 (en) * | 1997-10-24 | 2002-07-30 | Mount Sinai Hospital Corporation | Methods and compositions for modulating ubiquitin dependent proteolysis |
US5968747A (en) * | 1997-12-12 | 1999-10-19 | Incyte Pharmaceuticals, Inc. | Ubiquitin-like conjugating protein |
WO1999032514A2 (en) | 1997-12-19 | 1999-07-01 | Warner-Lambert Company | Sag: sensitive to apoptosis gene |
GB9727277D0 (en) | 1997-12-23 | 1998-02-25 | Medical Res Council | Assay |
US6165731A (en) * | 1998-01-22 | 2000-12-26 | California Institute Of Technology | Assay for the ubiquitination-promoting activity of human proteins |
US5976849A (en) * | 1998-02-05 | 1999-11-02 | Zeneca Limited | Human E3 ubiquitin protein ligase |
US6413725B1 (en) * | 1998-08-07 | 2002-07-02 | California Institute Of Technology | Biochemical assay to monitor the ubiquitin ligase activities of cullins |
AU2342900A (en) * | 1998-09-23 | 2000-05-01 | Cleveland Clinic Foundation, The | Novel interferon stimulated and repressed genes |
WO2000022110A2 (en) | 1998-10-09 | 2000-04-20 | President And Fellows Of Harvard College | Targeted proteolysis by recruitment to ubiquitin protein ligases |
US6306663B1 (en) * | 1999-02-12 | 2001-10-23 | Proteinex, Inc. | Controlling protein levels in eucaryotic organisms |
WO2000050631A2 (en) | 1999-02-25 | 2000-08-31 | Cyclacel Limited | Compositions and methods for monitoring the modification of natural binding partners |
CA2362520A1 (en) | 1999-02-26 | 2000-08-31 | Joan W. Conaway | Novel component of von hippel-lindau tumor suppressor complex and scf ubiquitin ligase |
CA2296792A1 (en) | 1999-02-26 | 2000-08-26 | Genset S.A. | Expressed sequence tags and encoded human proteins |
EP1165787A2 (de) | 1999-03-31 | 2002-01-02 | The University of North Carolina at Chapel Hill | Cullin regulatoren roc1 und roc2, dafür kodierende dna, und methoden zur ihrer verwendug |
JP2001192152A (ja) * | 2000-01-11 | 2001-07-17 | Fuji Photo Film Co Ltd | 給紙マガジン |
US6740495B1 (en) | 2000-04-03 | 2004-05-25 | Rigel Pharmaceuticals, Inc. | Ubiquitin ligase assay |
WO2001097830A1 (en) | 2000-06-22 | 2001-12-27 | Mount Sinai School Of Medicine | Modification of mdm2 activity |
EP1167539A1 (de) | 2000-06-29 | 2002-01-02 | Boehringer Ingelheim International GmbH | Verfahren zur Identifizierung von Verbindungen die das Anaphae-fördernde Komplex hemmen |
US6526205B1 (en) * | 2000-10-13 | 2003-02-25 | Agilent Technologies, Inc. | Method and apparatus for the passive alignment of optical components |
DE10108263A1 (de) | 2001-02-21 | 2002-09-05 | Max Planck Gesellschaft | Analyse von Modifizierungen und Demodifizierungen von Proteinen mit Ubiquitinverwandten Proteinen mittels FRET (Fluorescence Resonance Energy Transfer) |
-
2000
- 2000-04-03 US US09/542,497 patent/US6740495B1/en not_active Expired - Lifetime
-
2001
- 2001-04-03 EP EP01924655A patent/EP1268847B1/de not_active Expired - Lifetime
- 2001-04-03 WO PCT/US2001/010906 patent/WO2001075145A2/en active IP Right Grant
- 2001-04-03 AT AT01924655T patent/ATE377654T1/de active
- 2001-04-03 DE DE60131262T patent/DE60131262T2/de not_active Expired - Lifetime
- 2001-04-03 JP JP2001573019A patent/JP4095805B2/ja not_active Expired - Fee Related
- 2001-04-03 US US09/826,312 patent/US6737244B2/en not_active Expired - Lifetime
- 2001-04-03 AU AU2001251291A patent/AU2001251291A1/en not_active Abandoned
- 2001-04-03 DK DK01924655T patent/DK1268847T3/da active
- 2001-04-03 ES ES01924655T patent/ES2296744T3/es not_active Expired - Lifetime
-
2004
- 2004-04-28 US US10/835,096 patent/US7022493B2/en not_active Expired - Lifetime
-
2006
- 2006-01-09 US US11/328,747 patent/US7781182B2/en not_active Expired - Fee Related
- 2006-10-04 US US11/538,619 patent/US20090263831A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
AU2001251291A1 (en) | 2001-10-15 |
DE60131262T2 (de) | 2008-08-28 |
DE60131262D1 (en) | 2007-12-20 |
US20060105409A1 (en) | 2006-05-18 |
JP2003529372A (ja) | 2003-10-07 |
US7781182B2 (en) | 2010-08-24 |
US20020042083A1 (en) | 2002-04-11 |
DK1268847T3 (da) | 2008-03-10 |
US6740495B1 (en) | 2004-05-25 |
EP1268847B1 (de) | 2007-11-07 |
US7022493B2 (en) | 2006-04-04 |
WO2001075145A3 (en) | 2002-07-25 |
EP1268847A2 (de) | 2003-01-02 |
US20050032139A1 (en) | 2005-02-10 |
US6737244B2 (en) | 2004-05-18 |
ATE377654T1 (de) | 2007-11-15 |
US20090263831A1 (en) | 2009-10-22 |
ES2296744T3 (es) | 2008-05-01 |
WO2001075145A2 (en) | 2001-10-11 |
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