JP3779564B2 - Aeration culture tank - Google Patents

Aeration culture tank Download PDF

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JP3779564B2
JP3779564B2 JP2001197728A JP2001197728A JP3779564B2 JP 3779564 B2 JP3779564 B2 JP 3779564B2 JP 2001197728 A JP2001197728 A JP 2001197728A JP 2001197728 A JP2001197728 A JP 2001197728A JP 3779564 B2 JP3779564 B2 JP 3779564B2
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culture tank
gas phase
air
sensor
aeration
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JP2003009843A (en
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徹 春日井
隆盛 中野
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Hitachi Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/14—Pressurized fluid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/02—Means for regulation, monitoring, measurement or control, e.g. flow regulation of foam

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  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
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Abstract

PROBLEM TO BE SOLVED: To provide an aeration culture tank which can defoam without using a special installation, a special chemical agent, or the like. SOLUTION: When a sensor 2 detects a foam, air 12 or steam 11 is automatically blown in the gas phase portion of the culture tank 3 to defoam.

Description

【０００１】
【発明の属する技術分野】
本発明は、通気培養を行ない、液面に発生した泡を消すことが可能な通気培養槽に関する。
【０００２】
【従来の技術】
培養槽で通気培養を行なう時に、培地の種類によって通気による泡が消えずにそのまま液面に残ってしまい、タンクノズルから外に出てフィルター等を汚してしまう現象が課題となっていた。
【０００３】
従来、通気培養槽において、通気により生じた泡を消す為には、消泡剤が使用されることが多かった。
【０００４】
【発明が解決しようとする課題】
しかしながら、消泡剤を使用する従来技術において、培養している微生物等に対して使用した消泡剤が悪影響を与える場合や、精製工程で培養時に使用した消泡剤の存在が問題になる場合には、消泡剤を使用することができなかった。
また、消泡翼も用いられる場合もあるが、液の特性によっては翼で衝撃を与えるだけでは泡が消えない場合が多かった。
【０００５】
このように、消泡する有効な手段が存在しない場合には、生産性を犠牲にして発泡しない程度まで通気量や攪拌翼回転数を低く押さえて培養を行なっていた。また、消泡剤が使用できる場合でも、消泡剤とそれを投入する為の装置または消泡翼の様に消泡以外には必要のない設備を追加しなければならないという課題を有していた。
【０００６】
本発明の目的は、上記課題を解決すべく、培養している微生物に対する毒性や培養後の精製工程への悪影響を残すことなく、消泡を行なうことのできる通気培養槽を提供することにある。
【０００７】
また、本発明の他の目的は、消泡専用に部品や設備を設けることなく、消泡を可能にした通気培養槽を提供することにある。
【０００８】
【課題を解決するための手段】
上記目的を達成する為に、本発明は、内部に液相部及びその上方の気相部を有する通気培養槽であって、前記液相部内に配置され、気泡を発生させるために前記液相部に空気を送り込む供給手段と、前記気相部から排気される気体の排気ライン上に配置され、この排気ラインからの前記気体の排気を調節する排気用バルブと、前記液相部の液面に泡が発生したことを検知するセンサとを備え、このセンサからの出力を受けて前記排気用バルブを調節して前記気相部内の圧力を上昇させた後、前記センサにより前記泡の発生が検知された際に前記気相部に空気を供給することを特徴とする。
また、本発明は、前記通気培養槽であって、前記センサからの出力を受けて前記排気用バルブを調節して前記気相部内の圧力を上昇させた後、前記センサにより前記泡の発生が検知された際に空気の供給を前記液相部から前記気相部に切り替えることを特徴とする。
また、本発明は、前記通気培養槽であって、前記供給手段と連通された前記空気の供給経路上に配置され、この空気の供給を調節する供給用バルブを備え、前記センサからの出力を受けて前記排気用バルブを調節して前記気相部内の圧力を上昇させた後、前記供給用バルブを閉じて前記気相部に空気を供給することを特徴とする。
また、本発明は、前記通気培養槽であって、前記気相部に空気を供給して後、この気相部に蒸気を供給することを特徴とする。
【００１１】
【発明の実施の形態】
本発明に係る通気培養槽（通気攪拌槽）の実施の形態について図１を用いて説明する。
【００１２】
通気培養槽（通気攪拌翼）３には、駆動源１０によって回転される攪拌翼９が液相中に設置される。消泡センサー２は、通気培養槽３において、通気培養中での発泡を検知するものである。更に、通気培養槽３は、気泡を発生させるためのスパージャー１４等が設置されている。スチーム１１は、通常、培養前に滅菌するために通気培養槽３内にバルブＣ６等を介して送り込まれる。圧縮空気(エアー)１２は、通常、例えばスパージャー１４から気泡を発生させるためにバルブＤ７およびバルブＢ５を介して送り込まれる。その結果、通気培養槽３内では、攪拌翼９によって液体と気泡との混合が行われ、円滑な培養が行われることになる。
【００１３】
そこで、通気培養槽３で通気培養中に消泡センサー２で発泡を検知した場合、制御装置１により排気ライン１３のコントロールバルブ８の開度を下げることで培養槽３内の圧力を上げる。このように、任意の圧力まで上げても発泡が続いている場合には、バルブＢ５を閉じてバルブＡ４を開くことによって空気１２の培養槽３内への入り口を液中から気相中に切り替える。１分程度気相中に空気１２を通気しても消泡センサー２で泡の検知が続いている場合、バルブＤ７を閉じて空気１２の供給を止め、バルブＣ６を開閉することによってスチーム１１を培養槽３の気相部に一瞬だけ吹込む。気相部に空気１２又はスチーム１１を入れるノズル１５は液の圧送用のノズルと共用可能であり、スチーム１１も培養槽３の滅菌用のスチームを使用できる為、消泡専用に部品や設備等を追加する必要はない。
【００１４】
以上説明したように、通気培養槽３において、液面に泡が発生したことを検知するセンサー２を気相部に備え、センサー２により発泡を検知した際には自動的に気相部にエアー１２またはスチーム１１のいずかもしくは両方を吹込む、または排気ライン１３のコントロールバルブ８の開度を下げることによって内圧を上げて運転を行うことにより、消泡を行なうことが可能となる。
【００１５】
なお、制御装置１は、発泡を検知するセンサー２からの信号を基に、バルブ４〜７、およびコントロールバルブ８を制御するものである。
【００１６】
【発明の効果】
本発明によれば、通気培養槽には必要な圧縮空気またはスチームを用いることで、消泡用の設備を設置せずに消泡を行なうことが出来る効果を奏する。
【００１７】
また、本発明によれば、培養している微生物等の生育を阻害したり、生存を脅かしたりする、または精製工程で精製の邪魔になる消泡剤を、消泡を行なった後に培地内に残さないため、通気培養槽において従来は消泡できないような条件でも消泡を行なうことが出来る効果を奏する。
【図面の簡単な説明】
【図１】本発明に係る通気培養槽の一実施の形態の構成を示す図である。
【符号の説明】
１…制御装置、２…消泡センサー、３…培養槽、４…バルブＡ、５…バルブＢ、６…バルブＣ、７…バルブＤ、８…コントロールバルブ、１１…スチーム、１２…空気（エアー）、１３…排気ライン、１４…スパージャー、１５…ノズル。[0001]
BACKGROUND OF THE INVENTION
The present invention relates to an aeration culture tank capable of performing aeration culture and extinguishing bubbles generated on a liquid surface.
[0002]
[Prior art]
When aeration culture is carried out in a culture tank, the phenomenon that bubbles caused by aeration remain on the liquid surface as they are depending on the type of medium, and the filter etc. is contaminated by going out of the tank nozzle.
[0003]
Conventionally, an antifoaming agent has been often used in an aerated culture tank in order to eliminate bubbles generated by aeration.
[0004]
[Problems to be solved by the invention]
However, in the conventional technology that uses antifoaming agents, when the antifoaming agent used for the microorganisms being cultured has an adverse effect, or when the presence of the antifoaming agent used during culturing in the purification process becomes a problem The antifoaming agent could not be used.
In some cases, a defoaming wing is also used, but depending on the characteristics of the liquid, the foam often does not disappear only by applying an impact with the wing.
[0005]
Thus, when there was no effective means for defoaming, the culture was carried out with the aeration rate and the number of rotations of the stirring blade being kept low to the extent that foaming would not occur at the expense of productivity. In addition, even when an antifoaming agent can be used, there is a problem that an antifoaming agent and a device for introducing the antifoaming agent or an equipment necessary for defoaming such as an antifoaming blade must be added. It was.
[0006]
An object of the present invention is to provide an aeration culture tank capable of defoaming without leaving the toxicity to microorganisms being cultured and the adverse effect on the purification process after the culture, in order to solve the above-mentioned problems. .
[0007]
Another object of the present invention is to provide an aeration culture tank that enables defoaming without providing parts or equipment dedicated to defoaming.
[0008]
[Means for Solving the Problems]
To achieve the above object, the present invention provides a aeration tank having a liquid phase and a vapor phase portion of its upper inside, is disposed in the liquid phase portion, the liquid phase in order to generate bubbles Supply means for sending air to the part, an exhaust valve arranged on the exhaust line of the gas exhausted from the gas phase part , for adjusting the exhaust of the gas from the exhaust line, and the liquid level of the liquid phase part A sensor for detecting the generation of bubbles, and adjusting the exhaust valve in response to the output from the sensor to increase the pressure in the gas phase, and then the generation of the bubbles by the sensor. When detected, air is supplied to the gas phase part .
Further, the present invention is the aeration culture tank, wherein after the output from the sensor is received and the exhaust valve is adjusted to increase the pressure in the gas phase, the bubble is generated by the sensor. When detected, the supply of air is switched from the liquid phase part to the gas phase part.
Further, the present invention is the aeration culture tank, and is provided on a supply path of the air communicated with the supply means, and includes a supply valve for adjusting the supply of the air, and outputs from the sensor. In response, after adjusting the exhaust valve to increase the pressure in the gas phase portion, the supply valve is closed to supply air to the gas phase portion.
Further, the present invention is the above-described aeration culture tank, characterized in that air is supplied to the gas phase part and then steam is supplied to the gas phase part.
[0011]
DETAILED DESCRIPTION OF THE INVENTION
An embodiment of an aeration culture tank (aeration stirring tank) according to the present invention will be described with reference to FIG.
[0012]
In the aeration culture tank (aeration stirring blade) 3, a stirring blade 9 rotated by a driving source 10 is installed in the liquid phase. The defoaming sensor 2 detects foaming during aeration culture in the aeration culture tank 3. Furthermore, the aeration culture tank 3 is provided with a sparger 14 for generating bubbles. The steam 11 is usually fed into the aeration culture tank 3 through a valve C6 or the like for sterilization before culturing. The compressed air (air) 12 is usually fed through a valve D7 and a valve B5 in order to generate bubbles from a sparger 14, for example. As a result, in the aeration culture tank 3, the liquid and bubbles are mixed by the stirring blade 9, and smooth culture is performed.
[0013]
Therefore, when foaming is detected by the defoaming sensor 2 during the aeration culture in the aeration culture tank 3, the pressure in the culture tank 3 is increased by lowering the opening of the control valve 8 of the exhaust line 13 by the control device 1. Thus, when foaming continues even if it raises to arbitrary pressures, the valve | bulb B5 is closed and the valve | bulb A4 is opened, and the entrance into the culture tank 3 of the air 12 is switched from a liquid to a gaseous phase. . If the defoaming sensor 2 continues to detect bubbles even if the air 12 is passed through the gas phase for about 1 minute, the valve 11 is closed to stop the supply of the air 12, and the steam C11 is opened and closed by opening and closing the valve C6. It blows in the gas phase part of the culture tank 3 only for a moment. The nozzle 15 for introducing the air 12 or the steam 11 into the gas phase part can be shared with the nozzle for pumping the liquid. Since the steam 11 can also use the steam for sterilization of the culture tank 3, parts and facilities dedicated to defoaming, etc. There is no need to add.
[0014]
As described above, in the aeration culture tank 3, the gas phase part is provided with the sensor 2 for detecting the occurrence of bubbles on the liquid surface, and when the foaming is detected by the sensor 2, air is automatically supplied to the gas phase part. It is possible to perform defoaming by injecting either or both of 12 and / or steam 11 or increasing the internal pressure by lowering the opening of the control valve 8 in the exhaust line 13.
[0015]
The control device 1 controls the valves 4 to 7 and the control valve 8 based on a signal from the sensor 2 that detects foaming.
[0016]
【The invention's effect】
According to the present invention, by using necessary compressed air or steam in the aeration culture tank, there is an effect that the defoaming can be performed without installing a defoaming facility.
[0017]
In addition, according to the present invention, an antifoaming agent that inhibits the growth of microorganisms or the like being cultured, threatens the survival, or interferes with purification in the purification step is added to the medium after defoaming. Since it does not remain, it has an effect that it can be defoamed even in conditions where defoaming cannot be performed in an aeration culture tank.
[Brief description of the drawings]
FIG. 1 is a diagram showing a configuration of an embodiment of an aeration culture tank according to the present invention.
[Explanation of symbols]
DESCRIPTION OF SYMBOLS 1 ... Control apparatus, 2 ... Defoaming sensor, 3 ... Culture tank, 4 ... Valve A, 5 ... Valve B, 6 ... Valve C, 7 ... Valve D, 8 ... Control valve, 11 ... Steam, 12 ... Air ), 13 ... exhaust line, 14 ... sparger, 15 ... nozzle.

Claims (4)

内部に液相部及びその上方に気相部を有する通気培養槽であって、
前記液相部内に配置され、気泡を発生させるために前記液相部に空気を送り込む供給手段と、前記気相部から排気される気体の排気ライン上に配置され、この排気ラインからの前記気体の排気を調節する排気用バルブと、前記液相部の液面に泡が発生したことを検知するセンサとを備え、このセンサからの出力を受けて前記排気用バルブを調節して前記気相部内の圧力を上昇させた後、前記センサにより前記泡の発生が検知された際に前記気相部に空気を供給する通気培養槽。 A aeration tank having a gas phase in the liquid phase and its upper inside,
A supply means arranged in the liquid phase part and for sending air to the liquid phase part to generate bubbles, and arranged on an exhaust line of gas exhausted from the gas phase part, and the gas from the exhaust line And an exhaust valve for adjusting the exhaust gas and a sensor for detecting that bubbles are generated on the liquid surface of the liquid phase part, and receiving the output from the sensor, the exhaust valve is adjusted to adjust the gas phase. An aeration culture tank for supplying air to the gas phase part when the generation of the bubbles is detected by the sensor after increasing the pressure in the part . 請求項１に記載の通気培養槽であって、
前記センサからの出力を受けて前記排気用バルブを調節して前記気相部内の圧力を上昇させた後、前記センサにより前記泡の発生が検知された際に空気の供給を前記液相部から前記気相部に切り替える通気培養槽。 The aeration culture tank according to claim 1,
After receiving the output from the sensor and adjusting the exhaust valve to increase the pressure in the gas phase portion, the supply of air from the liquid phase portion is detected when the generation of the bubbles is detected by the sensor. An aerated culture tank for switching to the gas phase part . 請求項２に記載の通気培養槽であって、
前記供給手段と連通された前記空気の供給経路上に配置され、この空気の供給を調節する供給用バルブを備え、
前記センサからの出力を受けて前記排気用バルブを調節して前記気相部内の圧力を上昇させた後、前記供給用バルブを閉じて前記気相部に空気を供給する通気培養槽。 The aeration culture tank according to claim 2,
A supply valve arranged on the supply path of the air communicated with the supply means and adjusting the supply of the air;
An aerated culture tank that receives the output from the sensor and adjusts the exhaust valve to increase the pressure in the gas phase, and then closes the supply valve and supplies air to the gas phase . 請求項１乃至３のいずれかに記載の通気培養槽であって、
前記気相部に空気を供給して後、この気相部に蒸気を供給する通気培養槽。 The aeration culture tank according to any one of claims 1 to 3,
An aerated culture tank that supplies air to the gas phase part and then supplies steam to the gas phase part .
JP2001197728A 2001-06-29 2001-06-29 Aeration culture tank Expired - Lifetime JP3779564B2 (en)

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